                         SEQUENCE LISTING

<110>  University of Utah Research Foundation
       Lim, Carol
       Matissek, Karina
 
<120>  TARGETING P53 AND ITS DNA-BINDING DOMAIN

<130>  21101.0297P1

<150>  61/936,790
<151>  2014-02-06

<150>  61/944,384
<151>  2014-02-25

<160>  30    

<170>  PatentIn version 3.5

<210>  1
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; primer

<400>  1
gcgcgcgcgc tccggaatgg aggagccgca gtca                                   34


<210>  2
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; primer

<400>  2
gcgcgcgcgc ggtacctcat ggtttcttct ttggctgggg                             40


<210>  3
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; primer

<400>  3
ccgggcccgc ggtccggaac ctaccagggc agctacg                                37


<210>  4
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; primer

<400>  4
ccgggcccgc ggggtacctt tcttgcggag attctcttcc t                           41


<210>  5
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; primer

<400>  5
gcgcgcgcgc ggtaccgctc ccagaatgcc agaggc                                 36


<210>  6
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; primer

<400>  6
gcgcgcgcgc ggatcctttc ttgcggagat tctctt                                 36


<210>  7
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; primer

<400>  7
gcgcgcgcgc gggatccggc tggatggaga atatttcacc cttca                       45


<210>  8
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; primer

<400>  8
gcgcgcgcgc gggatcctca cccagcctgg gcatcctt                               38


<210>  9
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; primer

<400>  9
tcccttccca gaaaaggtac cagggcagct acggt                                  35


<210>  10
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; primer

<400>  10
tcgagctatg gaaacatttt cagacctatg gaaactactt cctgaacgga attctg           56


<210>  11
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; primer

<400>  11
ttcactgaag acccaggtcc atccggagct cccagaatgc caga                        44


<210>  12
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; primer

<400>  12
tctcgagctc aagcttcgaa ttcattggac aatgg                                  35


<210>  13
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mitochondrial targeting signal

<400>  13

Arg Lys Gly Gln Glu Arg Phe Asn Arg Trp Phe Leu Thr Gly Met Thr 
1               5                   10                  15      


Val Ala Gly Val Val Leu Leu Gly Ser Leu Phe Ser Arg Lys 
            20                  25                  30  


<210>  14
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mitochondrial targeting signal

<400>  14

Gly Thr Pro Thr Trp Gln Thr Val Thr Ile Phe Val Ala Gly Val Leu 
1               5                   10                  15      


Thr Ala Ser Leu Thr Ile Trp Lys Lys Met Gly 
            20                  25          


<210>  15
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mitochondrial targeting signal

<400>  15

Gly Asn Gly Pro Ile Leu Asn Val Leu Val Val Leu Gly Val Val Leu 
1               5                   10                  15      


Leu Gly Gln Phe Val Val Arg Arg Phe Phe Lys Ser 
            20                  25              


<210>  16
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; primer

<400>  16
ccgggcccgc ggtccggaac ctaccagggc agctacg                                37


<210>  17
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; primer

<400>  17
ccgggcccgc ggggtacctt tcttgcggag attctcttcc t                           41


<210>  18
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; primer

<400>  18
cattctggga cagccgcgtc tgtgacttgc ac                                     32


<210>  19
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; primer

<400>  19
cagttcctgc atgggcggca tgaacgcgag gcccatcct                              39


<210>  20
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; primer

<400>  20
tttgtgcctg tcctggggca gaccggcgca ca                                     32


<210>  21
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; primer

<400>  21
accggcgcac agcggaagcg aatctccgc                                         29


<210>  22
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; primer

<400>  22
gcattctggg acagccgagt ctgtgacttg cacgta                                 36


<210>  23
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; oligo encoding mitochondrial targeting 
       signal

<400>  23
gatcctccta ctttgggacg cccacgtggc agaccgtgac catctttgtg gcgggagtgc       60

tcaccgcctc actcaccatc tggaagaaga tgggctgag                              99


<210>  24
<211>  393
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; wild type p53

<400>  24

Met Glu Glu Pro Gln Ser Asp Pro Ser Val Glu Pro Pro Leu Ser Gln 
1               5                   10                  15      


Glu Thr Phe Ser Asp Leu Trp Lys Leu Leu Pro Glu Asn Asn Val Leu 
            20                  25                  30          


Ser Pro Leu Pro Ser Gln Ala Met Asp Asp Leu Met Leu Ser Pro Asp 
        35                  40                  45              


Asp Ile Glu Gln Trp Phe Thr Glu Asp Pro Gly Pro Asp Glu Ala Pro 
    50                  55                  60                  


Arg Met Pro Glu Ala Ala Pro Pro Val Ala Pro Ala Pro Ala Ala Pro 
65                  70                  75                  80  


Thr Pro Ala Ala Pro Ala Pro Ala Pro Ser Trp Pro Leu Ser Ser Ser 
                85                  90                  95      


Val Pro Ser Gln Lys Thr Tyr Gln Gly Ser Tyr Gly Phe Arg Leu Gly 
            100                 105                 110         


Phe Leu His Ser Gly Thr Ala Lys Ser Val Thr Cys Thr Tyr Ser Pro 
        115                 120                 125             


Ala Leu Asn Lys Met Phe Cys Gln Leu Ala Lys Thr Cys Pro Val Gln 
    130                 135                 140                 


Leu Trp Val Asp Ser Thr Pro Pro Pro Gly Thr Arg Val Arg Ala Met 
145                 150                 155                 160 


Ala Ile Tyr Lys Gln Ser Gln His Met Thr Glu Val Val Arg Arg Cys 
                165                 170                 175     


Pro His His Glu Arg Cys Ser Asp Ser Asp Gly Leu Ala Pro Pro Gln 
            180                 185                 190         


His Leu Ile Arg Val Glu Gly Asn Leu Arg Val Glu Tyr Leu Asp Asp 
        195                 200                 205             


Arg Asn Thr Phe Arg His Ser Val Val Val Pro Tyr Glu Pro Pro Glu 
    210                 215                 220                 


Val Gly Ser Asp Cys Thr Thr Ile His Tyr Asn Tyr Met Cys Asn Ser 
225                 230                 235                 240 


Ser Cys Met Gly Gly Met Asn Arg Arg Pro Ile Leu Thr Ile Ile Thr 
                245                 250                 255     


Leu Glu Asp Ser Ser Gly Asn Leu Leu Gly Arg Asn Ser Phe Glu Val 
            260                 265                 270         


Arg Val Cys Ala Cys Pro Gly Arg Asp Arg Arg Thr Glu Glu Glu Asn 
        275                 280                 285             


Leu Arg Lys Lys Gly Glu Pro His His Glu Leu Pro Pro Gly Ser Thr 
    290                 295                 300                 


Lys Arg Ala Leu Pro Asn Asn Thr Ser Ser Ser Pro Gln Pro Lys Lys 
305                 310                 315                 320 


Lys Pro Leu Asp Gly Glu Tyr Phe Thr Leu Gln Ile Arg Gly Arg Glu 
                325                 330                 335     


Arg Phe Glu Met Phe Arg Glu Leu Asn Glu Ala Leu Glu Leu Lys Asp 
            340                 345                 350         


Ala Gln Ala Gly Lys Glu Pro Gly Gly Ser Arg Ala His Ser Ser His 
        355                 360                 365             


Leu Lys Ser Lys Lys Gly Gln Ser Thr Ser Arg His Lys Lys Leu Met 
    370                 375                 380                 


Phe Lys Thr Glu Gly Pro Asp Ser Asp 
385                 390             


<210>  25
<211>  201
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; DNA binding domain of p53

<400>  25

Ser Ser Ser Val Pro Ser Gln Lys Thr Tyr Gln Gly Ser Tyr Gly Phe 
1               5                   10                  15      


Arg Leu Gly Phe Leu His Ser Gly Thr Ala Lys Ser Val Thr Cys Thr 
            20                  25                  30          


Tyr Ser Pro Ala Leu Asn Lys Met Phe Cys Gln Leu Ala Lys Thr Cys 
        35                  40                  45              


Pro Val Gln Leu Trp Val Asp Ser Thr Pro Pro Pro Gly Thr Arg Val 
    50                  55                  60                  


Arg Ala Met Ala Ile Tyr Lys Gln Ser Gln His Met Thr Glu Val Val 
65                  70                  75                  80  


Arg Arg Cys Pro His His Glu Arg Cys Ser Asp Ser Asp Gly Leu Ala 
                85                  90                  95      


Pro Pro Gln His Leu Ile Arg Val Glu Gly Asn Leu Arg Val Glu Tyr 
            100                 105                 110         


Leu Asp Asp Arg Asn Thr Phe Arg His Ser Val Val Val Pro Tyr Glu 
        115                 120                 125             


Pro Pro Glu Val Gly Ser Asp Cys Thr Thr Ile His Tyr Asn Tyr Met 
    130                 135                 140                 


Cys Asn Ser Ser Cys Met Gly Gly Met Asn Arg Arg Pro Ile Leu Thr 
145                 150                 155                 160 


Ile Ile Thr Leu Glu Asp Ser Ser Gly Asn Leu Leu Gly Arg Asn Ser 
                165                 170                 175     


Phe Glu Val Arg Val Cys Ala Cys Pro Gly Arg Asp Arg Arg Thr Glu 
            180                 185                 190         


Glu Glu Asn Leu Arg Lys Lys Gly Glu 
        195                 200     


<210>  26
<211>  93
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; oligo capable of encoding a mitochondrial 
       targeting signal

<400>  26
gatccggcaa tggtcccatc ctgaacgtgc tggtggttct gggtgtggtt ctgttgggcc       60

agtttgtggt acgaagattc ttcaaatcat gag                                    93


<210>  27
<211>  93
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; oligo capable of encoding a mitochondrial 
       targeting signal

<400>  27
agaaagggcc aggagagatt caacagatgg ttcctgaccg gcatgaccgt ggccggcgtg       60

gtgctgctgg gcagcctgtt cagcagaaag tga                                    93


<210>  28
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; primer

<400>  28
gggacggaac agctttgagg tggctgtttg tgcctgtcct                             40


<210>  29
<211>  1182
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; nucleic acid sequence of p53

<400>  29
atggaggagc cgcagtcaga tcctagcgtc gagccccctc tgagtcagga aacattttca       60

gacctatgga aactacttcc tgaaaacaac gttctgtccc ccttgccgtc ccaagcaatg      120

gatgatttga tgctgtcccc ggacgatatt gaacaatggt tcactgaaga cccaggtcca      180

gatgaagctc ccagaatgcc agaggctgct ccccccgtgg cccctgcacc agcagctcct      240

acaccggcgg cccctgcacc agccccctcc tggcccctgt catcttctgt cccttcccag      300

aaaacctacc agggcagcta cggtttccgt ctgggcttct tgcattctgg gacagccaag      360

tctgtgactt gcacgtactc ccctgccctc aacaagatgt tttgccaact ggccaagacc      420

tgccctgtgc agctgtgggt tgattccaca cccccgcccg gcacccgcgt ccgcgccatg      480

gccatctaca agcagtcaca gcacatgacg gaggttgtga ggcgctgccc ccaccatgag      540

cgctgctcag atagcgatgg tctggcccct cctcagcatc ttatccgagt ggaaggaaat      600

ttgcgtgtgg agtatttgga tgacagaaac acttttcgac atagtgtggt ggtgccctat      660

gagccgcctg aggttggctc tgactgtacc accatccact acaactacat gtgtaacagt      720

tcctgcatgg gcggcatgaa ccggaggccc atcctcacca tcatcacact ggaagactcc      780

agtggtaatc tactgggacg gaacagcttt gaggtgcgtg tttgtgcctg tcctgggaga      840

gaccggcgca cagaggaaga gaatctccgc aagaaagggg agcctcacca cgagctgccc      900

ccagggagca ctaagcgagc actgcccaac aacaccagct cctctcccca gccaaagaag      960

aaaccactgg atggagaata tttcaccctt cagatccgtg ggcgtgagcg cttcgagatg     1020

ttccgagagc tgaatgaggc cttggaactc aaggatgccc aggctgggaa ggagccaggg     1080

gggagcaggg ctcactccag ccacctgaag tccaaaaagg gtcagtctac ctcccgccat     1140

aaaaaactca tgttcaagac agaagggcct gactcagact ga                        1182


<210>  30
<211>  570
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; oligo capable of encoding DNA binding domain
       of p53

<400>  30
acctaccagg gcagctacgg tttccgtctg ggcttcttgc attctgggac agccaagtct       60

gtgacttgca cgtactcccc tgccctcaac aagatgtttt gccaactggc caagacctgc      120

cctgtgcagc tgtgggttga ttccacaccc ccgcccggca cccgcgtccg cgccatggcc      180

atctacaagc agtcacagca catgacggag gttgtgaggc gctgccccca ccatgagcgc      240

tgctcagata gcgatggtct ggcccctcct cagcatctta tccgagtgga aggaaatttg      300

cgtgtggagt atttggatga cagaaacact tttcgacata gtgtggtggt gccctatgag      360

ccgcctgagg ttggctctga ctgtaccacc atccactaca actacatgtg taacagttcc      420

tgcatgggcg gcatgaaccg gaggcccatc ctcaccatca tcacactgga agactccagt      480

ggtaatctac tgggacggaa cagctttgag gtgcgtgttt gtgcctgtcc tgggagagac      540

cggcgcacag aggaagagaa tctccgcaag                                       570


