                         SEQUENCE LISTING

<110>  Schreiber, et al.
 
<120>  CHIMERIC ANTIGEN RECEPTORS RECOGNIZING CANCER-SPECIFIC TN 
       GLYCOPEPTIDE VARIANTS

<130>  27373/47860A PC

<150>  US 61/936,307
<151>  2014-02-05

<160>  29    

<170>  PatentIn version 3.5

<210>  1
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5E5 signal sequence, optimized

<400>  1
atggaatggt cttgggtgtt cctgttcttc ctgagcgtga ccaccggcgt gcacagc          57


<210>  2
<211>  57
<212>  DNA
<213>  Mus musculus


<220>
<221>  misc_feature
<223>  5E5 signal sequence, original

<400>  2
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa ctacaggtgt ccactcc          57


<210>  3
<211>  348
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5E5 heavy chain, optimized

<400>  3
caggtgcagc tgcagcagtc tgatgccgag ctcgtgaagc ctggcagcag cgtgaagatc       60

agctgcaagg ccagcggcta caccttcacc gaccacgcca tccactgggt caagcagaag      120

cctgagcagg gcctggaatg gatcggccac ttcagccccg gcaacaccga catcaagtac      180

aacgacaagt tcaagggcaa ggccaccctg accgtggaca gaagcagcag caccgcctac      240

atgcagctga acagcctgac cagcgaggac agcgccgtgt acttctgcaa gaccagcacc      300

ttctttttcg actactgggg ccagggcaca accctgacag tgtctagc                   348


<210>  4
<211>  348
<212>  DNA
<213>  Mus musculus


<220>
<221>  misc_feature
<223>  5E5 heavy chain, original

<400>  4
caggttcagc tgcagcagtc tgacgctgag ttggtgaaac ctgggtcttc agtgaagata       60

tcctgcaagg cttctggcta caccttcact gaccatgcta ttcactgggt gaagcagaag      120

cctgaacagg gcctggaatg gattggacat ttttctcccg gaaatactga tattaagtac      180

aatgacaagt tcaagggcaa ggccacactg actgtagaca gatcctccag cactgcctac      240

atgcagctca acagcctgac atctgaggat tctgcagtgt atttctgtaa aacgagtacg      300

tttttctttg actactgggg ccaaggcacc actctcacag tctcctca                   348


<210>  5
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5E5 light chain, optimized

<400>  5
gaactcgtga tgacccagag ccccagctct ctgacagtga cagccggcga gaaagtgacc       60

atgatctgca agtcctccca gagcctgctg aactccggcg accagaagaa ctacctgacc      120

tggtatcagc agaaacccgg ccagcccccc aagctgctga tcttttgggc cagcacccgg      180

gaaagcggcg tgcccgatag attcacaggc agcggctccg gcaccgactt taccctgacc      240

atcagctccg tgcaggccga ggacctggcc gtgtattact gccagaacga ctacagctac      300

cccctgacct tcggagccgg caccaagctg gaactgaag                             339


<210>  6
<211>  339
<212>  DNA
<213>  Mus musculus


<220>
<221>  misc_feature
<223>  5E5 light chain, original

<400>  6
gagctcgtca tgacccagtc tccatcctcc ctgactgtga cagcaggaga gaaggtcact       60

atgatctgca agtccagtca gagtctatta aacagtggag atcaaaagaa ctacttgacc      120

tggtaccagc agaaaccagg gcagcctcct aaacttttga tcttctgggc atcaacaagg      180

gagtctgggg tccctgatcg cttcacaggc agtggatctg gaacagattt cactctcacc      240

atcagcagtg tacaggctga agacctggca gtttattact gtcagaatga ttatagttat      300

ccgctcacgt tcggtgctgg gaccaaactg gagctgaaa                             339


<210>  7
<211>  789
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5E5 construct

<400>  7
atggaatggt cttgggtgtt cctgttcttc ctgagcgtga ccaccggcgt gcacagccag       60

gtgcagctgc agcagtctga tgccgagctc gtgaagcctg gcagcagcgt gaagatcagc      120

tgcaaggcca gcggctacac cttcaccgac cacgccatcc actgggtcaa gcagaagcct      180

gagcagggcc tggaatggat cggccacttc agccccggca acaccgacat caagtacaac      240

gacaagttca agggcaaggc caccctgacc gtggacagaa gcagcagcac cgcctacatg      300

cagctgaaca gcctgaccag cgaggacagc gccgtgtact tctgcaagac cagcaccttc      360

tttttcgact actggggcca gggcacaacc ctgacagtgt ctagcggcgg aggcggatct      420

ggcggcggag gatctggggg aggcggctct gaactcgtga tgacccagag ccccagctct      480

ctgacagtga cagccggcga gaaagtgacc atgatctgca agtcctccca gagcctgctg      540

aactccggcg accagaagaa ctacctgacc tggtatcagc agaaacccgg ccagcccccc      600

aagctgctga tcttttgggc cagcacccgg gaaagcggcg tgcccgatag attcacaggc      660

agcggctccg gcaccgactt taccctgacc atcagctccg tgcaggccga ggacctggcc      720

gtgtattact gccagaacga ctacagctac cccctgacct tcggagccgg caccaagctg      780

gaactgaag                                                              789


<210>  8
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3H4 signal sequence, optimized

<400>  8
atgggctggt cctgcatcat cctgtttctg gtggccacag ccaccggcgt gcacagc          57


<210>  9
<211>  342
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3H4 heavy chain, optimized

<400>  9
caggtgcagc tggaagaatc tggccctggc ctggtggccc ctagccagag cctgagcatc       60

acatgcaccg tgtccggctt ctccctgacc tcctatggcg tgtcatgggt gcgacagcct      120

ccaggcaagg gcctggaatg gctgggagtg atttggggcg acggcagcac caactaccac      180

agcgccctga tcagcagact gagcatctcc aaggacaaca gcaagagcca ggtgttcctg      240

aagctgaaca gcctgcagac cgacgacaca gccacatatt actgcgccaa gggcggctac      300

ttcgactact ggggccaggg cacaaccctg accgtgtcta gt                         342


<210>  10
<211>  342
<212>  DNA
<213>  Mus musculus


<220>
<221>  misc_feature
<223>  3H4 heavy chain, original

<400>  10
caggtgcagc tcgaggagtc aggacctggc ctggtggcgc cctcacagag cctgtccatc       60

acatgcactg tctcagggtt ctcattaacc agctatggtg taagctgggt tcgccagcct      120

ccaggaaagg gtctggagtg gctgggagta atatggggtg acgggagcac aaattatcat      180

tcagctctca tatccagact gagcatcagc aaggataact ccaagagcca agttttctta      240

aaactgaaca gtctgcaaac tgatgacaca gccacgtact actgtgccaa aggggggtac      300

tttgactact ggggccaagg caccactctc acagtctcct ca                         342


<210>  11
<211>  318
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3H4 light chain, optimized

<400>  11
cagattgtgc tgacacagag ccccgccatc atgagcgcta gccctggcga gaaagtgacc       60

atcacctgta gcgccagcag cagcgtgtcc tacatgcact ggttccagca gaagcccggc      120

accagcccca agctgtggat ctacagcacc agcaacctgg ccagcggcgt gcccgctaga      180

ttttctggca gcggctctgg caccagctac agcctgacca tcagcagaat ggaagccgag      240

gacgccgcca cctactactg ccagcagaga agcagctacc ccttcacctt cggcagcggc      300

accaagctgg aaatcaag                                                    318


<210>  12
<211>  318
<212>  DNA
<213>  Mus musculus


<220>
<221>  misc_feature
<223>  3H4 light chain, original

<400>  12
caaattgttc tcacccagtc tccagcaatc atgtctgcat ctccagggga gaaggtcacc       60

ataacctgca gtgccagctc aagtgtaagt tacatgcact ggttccagca gaagccaggc      120

acttctccca aactctggat ttatagcaca tccaacctgg cttctggagt ccctgctcgc      180

ttcagtggca gtggatctgg gacctcttac tctctcacaa tcagccgaat ggaggctgaa      240

gatgctgcca cttattactg ccagcaaagg agtagttacc cattcacgtt cggctcgggg      300

acaaagttgg aaataaaa                                                    318


<210>  13
<211>  762
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3H4 construct

<400>  13
atgggctggt cctgcatcat cctgtttctg gtggccacag ccaccggcgt gcacagccag       60

attgtgctga cacagagccc cgccatcatg agcgctagcc ctggcgagaa agtgaccatc      120

acctgtagcg ccagcagcag cgtgtcctac atgcactggt tccagcagaa gcccggcacc      180

agccccaagc tgtggatcta cagcaccagc aacctggcca gcggcgtgcc cgctagattt      240

tctggcagcg gctctggcac cagctacagc ctgaccatca gcagaatgga agccgaggac      300

gccgccacct actactgcca gcagagaagc agctacccct tcaccttcgg cagcggcacc      360

aagctggaaa tcaagggcgg aggcggatct ggcggcggag gatctggggg aggcggctct      420

caggtgcagc tggaagaatc tggccctggc ctggtggccc ctagccagag cctgagcatc      480

acatgcaccg tgtccggctt ctccctgacc tcctatggcg tgtcatgggt gcgacagcct      540

ccaggcaagg gcctggaatg gctgggagtg atttggggcg acggcagcac caactaccac      600

agcgccctga tcagcagact gagcatctcc aaggacaaca gcaagagcca ggtgttcctg      660

aagctgaaca gcctgcagac cgacgacaca gccacatatt actgcgccaa gggcggctac      720

ttcgactact ggggccaggg cacaaccctg accgtgtcta gt                         762


<210>  14
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  14
ggcggaggcg gatctggcgg cggaggatct gggggaggcg gctct                       45


<210>  15
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  5E5 signal peptide

<400>  15

Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      


Val His Ser 
            


<210>  16
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  5E5 heavy chain

<400>  16

Gln Val Gln Leu Gln Gln Ser Asp Ala Glu Leu Val Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp His 
            20                  25                  30          


Ala Ile His Trp Val Lys Gln Lys Pro Glu Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly His Phe Ser Pro Gly Asn Thr Asp Ile Lys Tyr Asn Asp Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Arg Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Lys Thr Ser Thr Phe Phe Phe Asp Tyr Trp Gly Gln Gly Thr Thr Leu 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  17
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  5E5 light chain

<400>  17

Glu Leu Val Met Thr Gln Ser Pro Ser Ser Leu Thr Val Thr Ala Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Ile Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser 
            20                  25                  30          


Gly Asp Gln Lys Asn Tyr Leu Thr Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Phe Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Val Tyr Tyr Cys Gln Asn 
                85                  90                  95      


Asp Tyr Ser Tyr Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu 
            100                 105                 110         


Lys 
    


<210>  18
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3H4 signal peptide, optimized

<400>  18

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser 
            


<210>  19
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3H4 heavy chain

<400>  19

Gln Val Gln Leu Glu Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Thr Ser Tyr 
            20                  25                  30          


Gly Val Ser Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Gly Val Ile Trp Gly Asp Gly Ser Thr Asn Tyr His Ser Ala Leu Ile 
    50                  55                  60                  


Ser Arg Leu Ser Ile Ser Lys Asp Asn Ser Lys Ser Gln Val Phe Leu 
65                  70                  75                  80  


Lys Leu Asn Ser Leu Gln Thr Asp Asp Thr Ala Thr Tyr Tyr Cys Ala 
                85                  90                  95      


Lys Gly Gly Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val 
            100                 105                 110         


Ser Ser 
        


<210>  20
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3H4 light chain

<400>  20

Gln Ile Val Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Ile Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 
            20                  25                  30          


His Trp Phe Gln Gln Lys Pro Gly Thr Ser Pro Lys Leu Trp Ile Tyr 
        35                  40                  45              


Ser Thr Ser Asn Leu Ala Ser Gly Val Pro Ala Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg Met Glu Ala Glu 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Arg Ser Ser Tyr Pro Phe Thr 
                85                  90                  95      


Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys 
            100                 105     


<210>  21
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  21

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  22
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  22

Ser Ile Tyr Arg Tyr Tyr Gly Leu 
1               5               


<210>  23
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  23
gcggccgcca cc                                                           12


<210>  24
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  24
ctcgag                                                                   6


<210>  25
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide linker

<400>  25

Glu Glu Gly Glu Phe Ser Glu Ala Arg 
1               5                   


<210>  26
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide linker

<400>  26

Ala Lys Thr Thr Pro Pro Lys Leu Glu Glu Gly Glu Phe Ser Glu Ala 
1               5                   10                  15      


Arg Val 
        


<210>  27
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  27

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp 
            20      


<210>  28
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Myc tag

<400>  28

Gly Gly Pro Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Asn 
1               5                   10                  


<210>  29
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic His tag

<400>  29

His His His His His His 
1               5       


