                         SEQUENCE LISTING

<110>  Cote, Gregory L
       Skory, Christopher  D.
 
<120>  MODIFIED GLUCANSUCRASE AND RELATED METHODS

<130>  0127.13

<150>  61/936,663
<151>  2014-02-06

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  1473
<212>  PRT
<213>  glucansucrase

<400>  1

Asp Val Ser Gln Asn Asn Gly Val Val Val Ala Thr Ala Val Asp Gln 
1               5                   10                  15      


Ser Asn Leu Asp Ala Thr Thr Ser Asp Lys Ser Ile Thr Thr Asp Asp 
            20                  25                  30          


Lys Ala Ala Thr Thr Ala Ala Thr Ser Thr Asp Asp Lys Ala Thr Thr 
        35                  40                  45              


Thr Val Ala Thr Ser Thr Asp Asp Lys Asp Thr Thr Thr Ala Ala Thr 
    50                  55                  60                  


Ser Thr Asp Asp Lys Ala Thr Thr Thr Val Ala Thr Ser Thr Asp Asp 
65                  70                  75                  80  


Lys Ala Thr Thr Thr Ala Ala Thr Ser Thr Asp Asp Lys Ala Ala Thr 
                85                  90                  95      


Thr Ala Ala Thr Ser Thr Asp Asp Lys Ala Ala Thr Thr Ala Ala Thr 
            100                 105                 110         


Ser Thr Asp Asp Lys Ala Ala Thr Thr Ala Asp Thr Ser Thr Asp Asp 
        115                 120                 125             


Lys Ala Ala Thr Thr Ala Ala Thr Ser Thr Asp Asp Lys Ala Thr Thr 
    130                 135                 140                 


Thr Ala Ala Thr Ser Thr Asp Asp Lys Thr Ala Thr Thr Val Gly Thr 
145                 150                 155                 160 


Ser Asp Asn Asn Asn Ser Ala Thr Ala Ser Asp Lys Asp Val Ser Ser 
                165                 170                 175     


Ser Ala Gln Lys Ser Gln Thr Ile Asp Asn Asn Ser Lys Thr Ala Asp 
            180                 185                 190         


Thr Thr Ala Ala Leu Glu Ala Ser Ser Lys Asn Leu Lys Thr Ile Asp 
        195                 200                 205             


Gly Lys Thr Tyr Tyr Tyr Asp Asp Asp Asp Gln Val Lys Lys Asn Phe 
    210                 215                 220                 


Ala Thr Val Ile Asp Gly Lys Val Leu Tyr Phe Asp Lys Glu Thr Gly 
225                 230                 235                 240 


Ala Leu Ala Asp Thr Asn Asp Tyr Gln Phe Leu Glu Gly Leu Thr Ser 
                245                 250                 255     


Glu Asn Asn Thr Tyr Thr Glu His Asn Ala Ser Val Gly Thr Ser Ser 
            260                 265                 270         


Asp Ser Tyr Thr Asn Val Asp Gly Tyr Leu Thr Ala Asp Ser Trp Tyr 
        275                 280                 285             


Arg Pro Lys Asp Ile Leu Val Asn Gly Gln Asn Trp Glu Ser Ser Lys 
    290                 295                 300                 


Asp Asp Asp Leu Arg Pro Leu Leu Met Thr Trp Trp Pro Asp Lys Ala 
305                 310                 315                 320 


Thr Gln Val Asn Tyr Leu Asn Ala Met Lys Tyr Leu Asp Ala Thr Glu 
                325                 330                 335     


Thr Glu Thr Val Tyr Thr Ser Asp Asp Ser Gln Asp Ala Leu Asn Lys 
            340                 345                 350         


Ala Ala Gln Asn Ile Gln Val Lys Ile Glu Glu Lys Ile Ser Gln Glu 
        355                 360                 365             


Gly Gln Thr Gln Trp Leu Lys Asp Asp Ile Ser Lys Phe Val Asp Ser 
    370                 375                 380                 


Gln Ser Asn Trp Asn Ile Ala Ser Glu Ser Lys Gly Thr Asp His Leu 
385                 390                 395                 400 


Gln Gly Gly Ala Leu Leu Tyr Val Asn Ser Asp Lys Thr Pro Asp Ala 
                405                 410                 415     


Asn Ser Asp Tyr Arg Leu Leu Asn Arg Thr Pro Thr Asn Gln Thr Gly 
            420                 425                 430         


Thr Pro Leu Tyr Thr Thr Asp Pro Thr Gln Gly Gly Tyr Asp Phe Leu 
        435                 440                 445             


Leu Ala Asn Asp Val Asp Asn Ser Asn Pro Val Val Gln Ala Glu Gln 
    450                 455                 460                 


Leu Asn Trp Met Tyr Tyr Leu Leu Asn Phe Gly Ser Ile Thr Asn Asn 
465                 470                 475                 480 


Asp Ala Asp Ala Asn Phe Asp Ser Ile Arg Val Asp Ala Val Asp Asn 
                485                 490                 495     


Val Asp Ala Asp Leu Leu Gln Ile Ala Ala Asp Tyr Phe Lys Ala Ala 
            500                 505                 510         


Tyr Gly Val Asp Lys Ser Asp Ala Ile Ser Asn Gln His Val Ser Ile 
        515                 520                 525             


Leu Glu Asp Trp Ser Asp Asn Asp Ala Glu Tyr Val Lys Asp Asn Gly 
    530                 535                 540                 


Asp Asn Gln Leu Ser Met Asp Asn Lys Leu Arg Leu Ser Leu Lys Tyr 
545                 550                 555                 560 


Ser Leu Thr Met Pro Ala Val Asp Gln Tyr Gly Asn Lys Arg Ser Gly 
                565                 570                 575     


Leu Glu Pro Phe Leu Thr Asn Ser Leu Val Asp Arg Thr Asn Asp Ser 
            580                 585                 590         


Thr Asp Asn Thr Ala Gln Pro Asn Tyr Ser Phe Val Arg Ala His Asp 
        595                 600                 605             


Ser Glu Val Gln Thr Val Ile Ala Glu Ile Ile Lys Gln Arg Ile Asp 
    610                 615                 620                 


Pro Asp Ser Asp Gly Leu Ser Pro Thr Met Asp Gln Leu Thr Glu Ala 
625                 630                 635                 640 


Phe Lys Ile Tyr Asn Ala Asp Gln Leu Lys Thr Asp Lys Glu Phe Thr 
                645                 650                 655     


Gln Tyr Asn Ile Pro Ser Thr Tyr Ala Thr Ile Leu Thr Asn Lys Asp 
            660                 665                 670         


Thr Val Pro Arg Val Tyr Tyr Gly Asp Met Tyr Thr Asp Asp Gly Gln 
        675                 680                 685             


Tyr Met Ala Thr Lys Ser Leu Tyr Tyr Asp Ala Ile Asp Thr Leu Leu 
    690                 695                 700                 


Lys Ser Arg Ile Lys Tyr Val Ser Gly Gly Gln Thr Met Ser Met Lys 
705                 710                 715                 720 


Tyr Met Gln Gly Asp Ser Ser Met Ala Ala Asp Ser Tyr Arg Gly Ile 
                725                 730                 735     


Leu Thr Ser Val Arg Tyr Gly Asn Gly Ala Met Thr Ala Thr Asp Ala 
            740                 745                 750         


Gly Thr Asn Glu Thr Arg Thr Gln Gly Ile Ala Val Ile Glu Ser Asn 
        755                 760                 765             


Asn Pro Asp Leu Lys Leu Ser Ser Thr Asp Gln Val Val Val Asp Met 
    770                 775                 780                 


Gly Ile Ala His Lys Asn Gln Ala Tyr Arg Pro Ala Leu Leu Thr Thr 
785                 790                 795                 800 


Lys Asp Gly Ile Asp Thr Tyr Val Ser Asp Ser Asp Val Ser Gln Ser 
                805                 810                 815     


Leu Ile Arg Tyr Thr Asn Ser Asn Gly Gln Leu Ile Phe Asn Ser Ser 
            820                 825                 830         


Asp Ile Val Gly Thr Ala Asn Pro Gln Val Ser Gly Tyr Leu Ala Val 
        835                 840                 845             


Trp Val Pro Val Gly Ala Ser Asp Thr Gln Asp Ala Arg Thr Glu Ser 
    850                 855                 860                 


Ser Thr Ala Thr Thr Ala Asp Gly Gln Thr Leu His Ser Asn Ala Ala 
865                 870                 875                 880 


Leu Asp Ser Gln Val Ile Tyr Glu Ser Phe Ser Asn Phe Gln Ser Thr 
                885                 890                 895     


Pro Thr Thr Glu Ala Glu Tyr Ala Asn Val Gln Ile Ala Asn Asn Thr 
            900                 905                 910         


Asp Leu Tyr Lys Ser Trp Gly Ile Thr Asn Phe Glu Phe Pro Pro Gln 
        915                 920                 925             


Tyr Arg Ser Ser Thr Asp Ser Ser Phe Leu Asp Ser Ile Ile Gln Asn 
    930                 935                 940                 


Gly Tyr Ala Phe Thr Asp Arg Tyr Asp Leu Gly Phe Asn Thr Pro Thr 
945                 950                 955                 960 


Lys Tyr Gly Thr Val Asp Gln Leu Arg Thr Ala Ile Lys Ala Leu His 
                965                 970                 975     


Ala Thr Gly Ile Lys Ala Met Ala Asp Trp Val Pro Asp Gln Ile Tyr 
            980                 985                 990         


Asn Leu Thr Gly Lys Glu Val Val  Ala Val Gln Arg Val  Asn Asn Ser 
        995                 1000                 1005             


Gly Ile  Tyr Asn Gln Asp Ser  Val Ile Asn Lys Thr  Leu Tyr Ala 
    1010                 1015                 1020             


Ser Gln  Thr Val Gly Gly Gly  Glu Tyr Gln Ala Leu  Tyr Gly Gly 
    1025                 1030                 1035             


Glu Phe  Leu Asp Glu Ile Lys  Lys Leu Tyr Pro Ser  Leu Phe Glu 
    1040                 1045                 1050             


Lys Asn  Gln Ile Ser Thr Gly  Val Pro Met Asp Ala  Ser Glu Lys 
    1055                 1060                 1065             


Ile Lys  Glu Trp Ser Ala Lys  Tyr Phe Asn Gly Thr  Asn Ile Gln 
    1070                 1075                 1080             


Gly Arg  Gly Ala Tyr Tyr Val  Leu Lys Asp Trp Ala  Thr Asn Glu 
    1085                 1090                 1095             


Tyr Phe  Lys Val Ser Thr Ser  Ser Asn Ser Ser Val  Phe Leu Pro 
    1100                 1105                 1110             


Lys Gln  Leu Thr Asn Glu Glu  Ser Asn Thr Gly Phe  Ile Ser Thr 
    1115                 1120                 1125             


Asp Gly  Gly Met Thr Tyr Tyr  Ser Thr Ser Gly Tyr  Gln Ala Lys 
    1130                 1135                 1140             


Asp Thr  Phe Ile Gln Asp Asp  Lys Ser Asn Trp Tyr  Tyr Phe Asp 
    1145                 1150                 1155             


Lys Asn  Gly Tyr Met Thr Tyr  Gly Phe Gln Thr Val  Asn Asp Asn 
    1160                 1165                 1170             


Asn Tyr  Tyr Phe Leu Pro Asn  Gly Ile Glu Leu Gln  Asp Ala Ile 
    1175                 1180                 1185             


Leu Glu  Asp Ser Lys Gly Asn  Val Tyr Tyr Phe Asn  Gln Tyr Gly 
    1190                 1195                 1200             


Lys Gln  Ala Val Asp Gly Tyr  Tyr Met Leu Ala Asn  Lys Thr Trp 
    1205                 1210                 1215             


Arg Tyr  Phe Asp Lys Asn Gly  Val Met Ala Asn Ala  Gly Leu Thr 
    1220                 1225                 1230             


Thr Val  Thr Val Asp Gly Gln  Glu His Ile Gln Tyr  Phe Asp Lys 
    1235                 1240                 1245             


Asn Gly  Ile Gln Val Lys Gly  Thr Ser Val Lys Asp  Ala Asp Gly 
    1250                 1255                 1260             


Lys Leu  Arg Tyr Phe Asp Thr  Asp Ser Gly Asp Met  Val Thr Asn 
    1265                 1270                 1275             


Arg Phe  Gly Glu Asn Thr Asp  Gly Thr Trp Ser Tyr  Phe Gly Ala 
    1280                 1285                 1290             


Asp Gly  Ile Ala Val Thr Gly  Ala Gln Thr Ile Ser  Gly Gln Lys 
    1295                 1300                 1305             


Leu Phe  Phe Asp Ala Asp Gly  Gln Gln Ile Lys Gly  Lys Glu Ala 
    1310                 1315                 1320             


Thr Asp  Lys Lys Gly Lys Val  His Tyr Tyr Asp Ala  Asn Ser Gly 
    1325                 1330                 1335             


Glu Met  Ile Thr Asn Arg Phe  Glu Lys Leu Ser Asp  Gly Ser Trp 
    1340                 1345                 1350             


Ala Tyr  Phe Asn Lys Lys Gly  Asn Ile Val Thr Gly  Ala Gln Val 
    1355                 1360                 1365             


Ile Asn  Gly Gln His Leu Phe  Phe Glu Ser Asn Gly  Asn Gln Val 
    1370                 1375                 1380             


Lys Gly  Arg Glu Tyr Thr Ala  Thr Asp Gly Lys Met  Arg Tyr Tyr 
    1385                 1390                 1395             


Asp Ala  Asp Ser Gly Asp Met  Val Thr Asn Arg Phe  Glu Arg Ile 
    1400                 1405                 1410             


Ser Asp  Gly Ser Trp Ala Tyr  Phe Gly Ala Asn Gly  Val Ala Val 
    1415                 1420                 1425             


Thr Gly  Glu Gln Asn Ile Asn  Gly Gln Gln Leu Tyr  Phe Asp Ala 
    1430                 1435                 1440             


Asn Gly  His Gln Val Lys Gly  Ala Ala Val Thr Gln  Ala Asp Gly 
    1445                 1450                 1455             


Ser Gln  Lys Tyr Tyr Asp Ala  Asn Ser Gly Glu Met  Ile Lys Ser 
    1460                 1465                 1470             


<210>  2
<211>  46
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer construct


<220>
<221>  misc_feature
<222>  (22)..(24)
<223>  n is a, c, g, or t

<400>  2
gcacatgata gtgaagtaca annngttatt gctgaaatta ttaaac                      46


<210>  3
<211>  46
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer construct


<220>
<221>  misc_feature
<222>  (23)..(25)
<223>  n is a, c, g, or t

<400>  3
gtttaataat ttcagcaata acnnnttgta cttcactatc atgtgc                      46


