                         SEQUENCE LISTING

<110>  Buddle, Bryce
       Wedlock, Neil
       Rehm, Bernd
 
<120>  POLYMER PARTICLES AND USES THEREOF

<130>  694801 JBM

<150>  NZ 620682
<151>  2014-02-04

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  95
<212>  PRT
<213>  Mycobacterium bovis


<220>
<221>  MISC_FEATURE
<223>  ESAT6 antigen

<400>  1

Met Thr Glu Gln Gln Trp Asn Phe Ala Gly Ile Glu Ala Ala Ala Ser 
1               5                   10                  15      


Ala Ile Gln Gly Asn Val Thr Ser Ile His Ser Leu Leu Asp Glu Gly 
            20                  25                  30          


Lys Gln Ser Leu Thr Lys Leu Ala Ala Ala Trp Gly Gly Ser Gly Ser 
        35                  40                  45              


Glu Ala Tyr Gln Gly Val Gln Gln Lys Trp Asp Ala Thr Ala Thr Glu 
    50                  55                  60                  


Leu Asn Asn Ala Leu Gln Asn Leu Ala Arg Thr Ile Ser Glu Ala Gly 
65                  70                  75                  80  


Gln Ala Met Ala Ser Thr Glu Gly Asn Val Thr Gly Met Phe Ala 
                85                  90                  95  


<210>  2
<211>  100
<212>  PRT
<213>  Mycobacterium bovis


<220>
<221>  MISC_FEATURE
<223>  CFP10 antigen

<400>  2

Met Ala Glu Met Lys Thr Asp Ala Ala Thr Leu Ala Gln Glu Ala Gly 
1               5                   10                  15      


Asn Phe Glu Arg Ile Ser Gly Asp Leu Lys Thr Gln Ile Asp Gln Val 
            20                  25                  30          


Glu Ser Thr Ala Gly Ser Leu Gln Gly Gln Trp Arg Gly Ala Ala Gly 
        35                  40                  45              


Thr Ala Ala Gln Ala Ala Val Val Arg Phe Gln Glu Ala Ala Asn Lys 
    50                  55                  60                  


Gln Lys Gln Glu Leu Asp Glu Ile Ser Thr Asn Ile Arg Gln Ala Gly 
65                  70                  75                  80  


Val Gln Tyr Ser Arg Ala Asp Glu Glu Gln Gln Gln Ala Leu Ser Ser 
                85                  90                  95      


Gln Met Gly Phe 
            100 


<210>  3
<211>  103
<212>  PRT
<213>  Mycobacterium bovis


<220>
<221>  MISC_FEATURE
<223>  Rv3615c antigen

<400>  3

Met Thr Glu Asn Leu Thr Val Gln Pro Glu Arg Leu Gly Val Leu Ala 
1               5                   10                  15      


Ser His His Asp Asn Ala Ala Val Asp Ala Ser Ser Gly Val Glu Ala 
            20                  25                  30          


Ala Ala Gly Leu Gly Glu Ser Val Ala Ile Thr His Gly Pro Tyr Cys 
        35                  40                  45              


Ser Gln Phe Asn Asp Thr Leu Asn Val Tyr Leu Thr Ala His Asn Ala 
    50                  55                  60                  


Leu Gly Ser Ser Leu His Thr Ala Gly Val Asp Leu Ala Lys Ser Leu 
65                  70                  75                  80  


Arg Ile Ala Ala Lys Ile Tyr Ser Glu Ala Asp Glu Ala Trp Arg Lys 
                85                  90                  95      


Ala Ile Asp Gly Leu Phe Thr 
            100             


<210>  4
<211>  102
<212>  PRT
<213>  Mycobacterium bovis


<220>
<221>  MISC_FEATURE
<223>  Rv3020c antigen

<400>  4

Gly Gly Gly Gly Gly Met Ser Leu Leu Asp Ala His Ile Pro Gln Leu 
1               5                   10                  15      


Ile Ala Ser His Thr Ala Phe Ala Ala Lys Ala Gly Leu Met Arg His 
            20                  25                  30          


Thr Ile Gly Gln Ala Glu Gln Gln Ala Met Ser Ala Gln Ala Phe His 
        35                  40                  45              


Gln Gly Glu Ser Ala Ala Ala Phe Gln Gly Ala His Ala Arg Phe Val 
    50                  55                  60                  


Ala Ala Ala Ala Lys Val Asn Thr Leu Leu Asp Ile Ala Gln Ala Asn 
65                  70                  75                  80  


Leu Gly Glu Ala Ala Gly Thr Tyr Val Ala Ala Asp Ala Ala Ala Ala 
                85                  90                  95      


Ser Ser Tyr Thr Gly Phe 
            100         


<210>  5
<211>  94
<212>  PRT
<213>  Mycobacterium bovis


<220>
<221>  MISC_FEATURE
<223>  Rv2346c antigen

<400>  5

Met Thr Ile Asn Tyr Gln Phe Gly Asp Val Asp Ala His Gly Ala Met 
1               5                   10                  15      


Ile Arg Ala Gln Ala Gly Leu Leu Glu Ala Glu His Gln Ala Ile Val 
            20                  25                  30          


Arg Asp Val Leu Ala Ala Gly Asp Phe Trp Gly Gly Ala Gly Ser Val 
        35                  40                  45              


Ala Cys Gln Glu Phe Ile Thr Gln Leu Gly Arg Asn Phe Gln Val Ile 
    50                  55                  60                  


Tyr Glu Gln Ala Asn Ala His Gly Gln Lys Val Gln Ala Ala Gly Asn 
65                  70                  75                  80  


Asn Met Ala Gln Thr Asp Ser Ala Val Gly Ser Ser Trp Ala 
                85                  90                  


<210>  6
<211>  198
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic fusion polypeptide


<220>
<221>  MISC_FEATURE
<223>  ESAT6-Rv3020c fusion

<400>  6

Glu Met Thr Glu Gln Gln Trp Asn Phe Ala Gly Ile Glu Ala Ala Ala 
1               5                   10                  15      


Ser Ala Ile Gln Gly Asn Val Thr Ser Ile His Ser Leu Leu Asp Glu 
            20                  25                  30          


Gly Lys Gln Ser Leu Thr Lys Leu Ala Ala Ala Trp Gly Gly Ser Gly 
        35                  40                  45              


Ser Glu Ala Tyr Gln Gly Val Gln Gln Lys Trp Asp Ala Thr Ala Thr 
    50                  55                  60                  


Glu Leu Asn Asn Ala Leu Gln Asn Leu Ala Arg Thr Ile Ser Glu Ala 
65                  70                  75                  80  


Gly Gln Ala Met Ala Ser Thr Glu Gly Asn Val Thr Gly Met Phe Ala 
                85                  90                  95      


Gly Gly Gly Gly Gly Met Ser Leu Leu Asp Ala His Ile Pro Gln Leu 
            100                 105                 110         


Ile Ala Ser His Thr Ala Phe Ala Ala Lys Ala Gly Leu Met Arg His 
        115                 120                 125             


Thr Ile Gly Gln Ala Glu Gln Gln Ala Met Ser Ala Gln Ala Phe His 
    130                 135                 140                 


Gln Gly Glu Ser Ala Ala Ala Phe Gln Gly Ala His Ala Arg Phe Val 
145                 150                 155                 160 


Ala Ala Ala Ala Lys Val Asn Thr Leu Leu Asp Ile Ala Gln Ala Asn 
                165                 170                 175     


Leu Gly Glu Ala Ala Gly Thr Tyr Val Ala Ala Asp Ala Ala Ala Ala 
            180                 185                 190         


Ser Ser Tyr Thr Gly Phe 
        195             


<210>  7
<211>  690
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic fusion polypeptide


<220>
<221>  MISC_FEATURE
<223>  CFP10-PhaC fusion

<400>  7

Met Ala Glu Met Lys Thr Asp Ala Ala Thr Leu Ala Gln Glu Ala Gly 
1               5                   10                  15      


Asn Phe Glu Arg Ile Ser Gly Asp Leu Lys Thr Gln Ile Asp Gln Val 
            20                  25                  30          


Glu Ser Thr Ala Gly Ser Leu Gln Gly Gln Trp Arg Gly Ala Ala Gly 
        35                  40                  45              


Thr Ala Ala Gln Ala Ala Val Val Arg Phe Gln Glu Ala Ala Asn Lys 
    50                  55                  60                  


Gln Lys Gln Glu Leu Asp Glu Ile Ser Thr Asn Ile Arg Gln Ala Gly 
65                  70                  75                  80  


Val Gln Tyr Ser Arg Ala Asp Glu Glu Gln Gln Gln Ala Leu Ser Ser 
                85                  90                  95      


Gln Met Gly Phe His Met Ala Thr Gly Lys Gly Ala Ala Ala Ser Thr 
            100                 105                 110         


Gln Glu Gly Lys Ser Gln Pro Phe Lys Val Thr Pro Gly Pro Phe Asp 
        115                 120                 125             


Pro Ala Thr Trp Leu Glu Trp Ser Arg Gln Trp Gln Gly Thr Glu Gly 
    130                 135                 140                 


Asn Gly His Ala Ala Ala Ser Gly Ile Pro Gly Leu Asp Ala Leu Ala 
145                 150                 155                 160 


Gly Val Lys Ile Ala Pro Ala Gln Leu Gly Asp Ile Gln Gln Arg Tyr 
                165                 170                 175     


Met Lys Asp Phe Ser Ala Leu Trp Gln Ala Met Ala Glu Gly Lys Ala 
            180                 185                 190         


Glu Ala Thr Gly Pro Leu His Asp Arg Arg Phe Ala Gly Asp Ala Trp 
        195                 200                 205             


Arg Thr Asn Leu Pro Tyr Arg Phe Ala Ala Ala Phe Tyr Leu Leu Asn 
    210                 215                 220                 


Ala Arg Ala Leu Thr Glu Leu Ala Asp Ala Val Glu Ala Asp Ala Lys 
225                 230                 235                 240 


Thr Arg Gln Arg Ile Arg Phe Ala Ile Ser Gln Trp Val Asp Ala Met 
                245                 250                 255     


Ser Pro Ala Asn Phe Leu Ala Thr Asn Pro Glu Ala Gln Arg Leu Leu 
            260                 265                 270         


Ile Glu Ser Gly Gly Glu Ser Leu Arg Ala Gly Val Arg Asn Met Met 
        275                 280                 285             


Glu Asp Leu Thr Arg Gly Lys Ile Ser Gln Thr Asp Glu Ser Ala Phe 
    290                 295                 300                 


Glu Val Gly Arg Asn Val Ala Val Thr Glu Gly Ala Val Val Phe Glu 
305                 310                 315                 320 


Asn Glu Tyr Phe Gln Leu Leu Gln Tyr Lys Pro Leu Thr Asp Lys Val 
                325                 330                 335     


His Ala Arg Pro Leu Leu Met Val Pro Pro Cys Ile Asn Lys Tyr Tyr 
            340                 345                 350         


Ile Leu Asp Leu Gln Pro Glu Ser Ser Leu Val Arg His Val Val Glu 
        355                 360                 365             


Gln Gly His Thr Val Phe Leu Val Ser Trp Arg Asn Pro Asp Ala Ser 
    370                 375                 380                 


Met Ala Gly Ser Thr Trp Asp Asp Tyr Ile Glu His Ala Ala Ile Arg 
385                 390                 395                 400 


Ala Ile Glu Val Ala Arg Asp Ile Ser Gly Gln Asp Lys Ile Asn Val 
                405                 410                 415     


Leu Gly Phe Cys Val Gly Gly Thr Ile Val Ser Thr Ala Leu Ala Val 
            420                 425                 430         


Leu Ala Ala Arg Gly Glu His Pro Ala Ala Ser Val Thr Leu Leu Thr 
        435                 440                 445             


Thr Leu Leu Asp Phe Ala Asp Thr Gly Ile Leu Asp Val Phe Val Asp 
    450                 455                 460                 


Glu Gly His Val Gln Leu Arg Glu Ala Thr Leu Gly Gly Gly Ala Gly 
465                 470                 475                 480 


Ala Pro Cys Ala Leu Leu Arg Gly Leu Glu Leu Ala Asn Thr Phe Ser 
                485                 490                 495     


Phe Leu Arg Pro Asn Asp Leu Val Trp Asn Tyr Val Val Asp Asn Tyr 
            500                 505                 510         


Leu Lys Gly Asn Thr Pro Val Pro Phe Asp Leu Leu Phe Trp Asn Gly 
        515                 520                 525             


Asp Ala Thr Asn Leu Pro Gly Pro Trp Tyr Cys Trp Tyr Leu Arg His 
    530                 535                 540                 


Thr Tyr Leu Gln Asn Glu Leu Lys Val Pro Gly Lys Leu Thr Val Cys 
545                 550                 555                 560 


Gly Val Pro Val Asp Leu Ala Ser Ile Asp Val Pro Thr Tyr Ile Tyr 
                565                 570                 575     


Gly Ser Arg Glu Asp His Ile Val Pro Trp Thr Ala Ala Tyr Ala Ser 
            580                 585                 590         


Thr Ala Leu Leu Ala Asn Lys Leu Arg Phe Val Leu Gly Ala Ser Gly 
        595                 600                 605             


His Ile Ala Gly Val Ile Asn Pro Pro Ala Lys Asn Lys Arg Ser His 
    610                 615                 620                 


Trp Thr Asn Asp Ala Leu Pro Glu Ser Pro Gln Gln Trp Leu Ala Gly 
625                 630                 635                 640 


Ala Ile Glu His His Gly Ser Trp Trp Pro Asp Trp Thr Ala Trp Leu 
                645                 650                 655     


Ala Gly Gln Ala Gly Ala Lys Arg Ala Ala Pro Ala Asn Tyr Gly Asn 
            660                 665                 670         


Ala Arg Tyr Arg Ala Ile Glu Pro Ala Pro Gly Arg Tyr Val Lys Ala 
        675                 680                 685             


Lys Ala 
    690 


<210>  8
<211>  693
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic fusion polypeptide


<220>
<221>  MISC_FEATURE
<223>  RV3615C-PhaC fusion

<400>  8

Met Thr Glu Asn Leu Thr Val Gln Pro Glu Arg Leu Gly Val Leu Ala 
1               5                   10                  15      


Ser His His Asp Asn Ala Ala Val Asp Ala Ser Ser Gly Val Glu Ala 
            20                  25                  30          


Ala Ala Gly Leu Gly Glu Ser Val Ala Ile Thr His Gly Pro Tyr Cys 
        35                  40                  45              


Ser Gln Phe Asn Asp Thr Leu Asn Val Tyr Leu Thr Ala His Asn Ala 
    50                  55                  60                  


Leu Gly Ser Ser Leu His Thr Ala Gly Val Asp Leu Ala Lys Ser Leu 
65                  70                  75                  80  


Arg Ile Ala Ala Lys Ile Tyr Ser Glu Ala Asp Glu Ala Trp Arg Lys 
                85                  90                  95      


Ala Ile Asp Gly Leu Phe Thr His Met Ala Thr Gly Lys Gly Ala Ala 
            100                 105                 110         


Ala Ser Thr Gln Glu Gly Lys Ser Gln Pro Phe Lys Val Thr Pro Gly 
        115                 120                 125             


Pro Phe Asp Pro Ala Thr Trp Leu Glu Trp Ser Arg Gln Trp Gln Gly 
    130                 135                 140                 


Thr Glu Gly Asn Gly His Ala Ala Ala Ser Gly Ile Pro Gly Leu Asp 
145                 150                 155                 160 


Ala Leu Ala Gly Val Lys Ile Ala Pro Ala Gln Leu Gly Asp Ile Gln 
                165                 170                 175     


Gln Arg Tyr Met Lys Asp Phe Ser Ala Leu Trp Gln Ala Met Ala Glu 
            180                 185                 190         


Gly Lys Ala Glu Ala Thr Gly Pro Leu His Asp Arg Arg Phe Ala Gly 
        195                 200                 205             


Asp Ala Trp Arg Thr Asn Leu Pro Tyr Arg Phe Ala Ala Ala Phe Tyr 
    210                 215                 220                 


Leu Leu Asn Ala Arg Ala Leu Thr Glu Leu Ala Asp Ala Val Glu Ala 
225                 230                 235                 240 


Asp Ala Lys Thr Arg Gln Arg Ile Arg Phe Ala Ile Ser Gln Trp Val 
                245                 250                 255     


Asp Ala Met Ser Pro Ala Asn Phe Leu Ala Thr Asn Pro Glu Ala Gln 
            260                 265                 270         


Arg Leu Leu Ile Glu Ser Gly Gly Glu Ser Leu Arg Ala Gly Val Arg 
        275                 280                 285             


Asn Met Met Glu Asp Leu Thr Arg Gly Lys Ile Ser Gln Thr Asp Glu 
    290                 295                 300                 


Ser Ala Phe Glu Val Gly Arg Asn Val Ala Val Thr Glu Gly Ala Val 
305                 310                 315                 320 


Val Phe Glu Asn Glu Tyr Phe Gln Leu Leu Gln Tyr Lys Pro Leu Thr 
                325                 330                 335     


Asp Lys Val His Ala Arg Pro Leu Leu Met Val Pro Pro Cys Ile Asn 
            340                 345                 350         


Lys Tyr Tyr Ile Leu Asp Leu Gln Pro Glu Ser Ser Leu Val Arg His 
        355                 360                 365             


Val Val Glu Gln Gly His Thr Val Phe Leu Val Ser Trp Arg Asn Pro 
    370                 375                 380                 


Asp Ala Ser Met Ala Gly Ser Thr Trp Asp Asp Tyr Ile Glu His Ala 
385                 390                 395                 400 


Ala Ile Arg Ala Ile Glu Val Ala Arg Asp Ile Ser Gly Gln Asp Lys 
                405                 410                 415     


Ile Asn Val Leu Gly Phe Cys Val Gly Gly Thr Ile Val Ser Thr Ala 
            420                 425                 430         


Leu Ala Val Leu Ala Ala Arg Gly Glu His Pro Ala Ala Ser Val Thr 
        435                 440                 445             


Leu Leu Thr Thr Leu Leu Asp Phe Ala Asp Thr Gly Ile Leu Asp Val 
    450                 455                 460                 


Phe Val Asp Glu Gly His Val Gln Leu Arg Glu Ala Thr Leu Gly Gly 
465                 470                 475                 480 


Gly Ala Gly Ala Pro Cys Ala Leu Leu Arg Gly Leu Glu Leu Ala Asn 
                485                 490                 495     


Thr Phe Ser Phe Leu Arg Pro Asn Asp Leu Val Trp Asn Tyr Val Val 
            500                 505                 510         


Asp Asn Tyr Leu Lys Gly Asn Thr Pro Val Pro Phe Asp Leu Leu Phe 
        515                 520                 525             


Trp Asn Gly Asp Ala Thr Asn Leu Pro Gly Pro Trp Tyr Cys Trp Tyr 
    530                 535                 540                 


Leu Arg His Thr Tyr Leu Gln Asn Glu Leu Lys Val Pro Gly Lys Leu 
545                 550                 555                 560 


Thr Val Cys Gly Val Pro Val Asp Leu Ala Ser Ile Asp Val Pro Thr 
                565                 570                 575     


Tyr Ile Tyr Gly Ser Arg Glu Asp His Ile Val Pro Trp Thr Ala Ala 
            580                 585                 590         


Tyr Ala Ser Thr Ala Leu Leu Ala Asn Lys Leu Arg Phe Val Leu Gly 
        595                 600                 605             


Ala Ser Gly His Ile Ala Gly Val Ile Asn Pro Pro Ala Lys Asn Lys 
    610                 615                 620                 


Arg Ser His Trp Thr Asn Asp Ala Leu Pro Glu Ser Pro Gln Gln Trp 
625                 630                 635                 640 


Leu Ala Gly Ala Ile Glu His His Gly Ser Trp Trp Pro Asp Trp Thr 
                645                 650                 655     


Ala Trp Leu Ala Gly Gln Ala Gly Ala Lys Arg Ala Ala Pro Ala Asn 
            660                 665                 670         


Tyr Gly Asn Ala Arg Tyr Arg Ala Ile Glu Pro Ala Pro Gly Arg Tyr 
        675                 680                 685             


Val Lys Ala Lys Ala 
    690             


<210>  9
<211>  685
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic fusion polypeptide


<220>
<221>  MISC_FEATURE
<223>  ESAT6-PhaC fusion

<400>  9

Met Thr Glu Gln Gln Trp Asn Phe Ala Gly Ile Glu Ala Ala Ala Ser 
1               5                   10                  15      


Ala Ile Gln Gly Asn Val Thr Ser Ile His Ser Leu Leu Asp Glu Gly 
            20                  25                  30          


Lys Gln Ser Leu Thr Lys Leu Ala Ala Ala Trp Gly Gly Ser Gly Ser 
        35                  40                  45              


Glu Ala Tyr Gln Gly Val Gln Gln Lys Trp Asp Ala Thr Ala Thr Glu 
    50                  55                  60                  


Leu Asn Asn Ala Leu Gln Asn Leu Ala Arg Thr Ile Ser Glu Ala Gly 
65                  70                  75                  80  


Gln Ala Met Ala Ser Thr Glu Gly Asn Val Thr Gly Met Phe Ala His 
                85                  90                  95      


Met Ala Thr Gly Lys Gly Ala Ala Ala Ser Thr Gln Glu Gly Lys Ser 
            100                 105                 110         


Gln Pro Phe Lys Val Thr Pro Gly Pro Phe Asp Pro Ala Thr Trp Leu 
        115                 120                 125             


Glu Trp Ser Arg Gln Trp Gln Gly Thr Glu Gly Asn Gly His Ala Ala 
    130                 135                 140                 


Ala Ser Gly Ile Pro Gly Leu Asp Ala Leu Ala Gly Val Lys Ile Ala 
145                 150                 155                 160 


Pro Ala Gln Leu Gly Asp Ile Gln Gln Arg Tyr Met Lys Asp Phe Ser 
                165                 170                 175     


Ala Leu Trp Gln Ala Met Ala Glu Gly Lys Ala Glu Ala Thr Gly Pro 
            180                 185                 190         


Leu His Asp Arg Arg Phe Ala Gly Asp Ala Trp Arg Thr Asn Leu Pro 
        195                 200                 205             


Tyr Arg Phe Ala Ala Ala Phe Tyr Leu Leu Asn Ala Arg Ala Leu Thr 
    210                 215                 220                 


Glu Leu Ala Asp Ala Val Glu Ala Asp Ala Lys Thr Arg Gln Arg Ile 
225                 230                 235                 240 


Arg Phe Ala Ile Ser Gln Trp Val Asp Ala Met Ser Pro Ala Asn Phe 
                245                 250                 255     


Leu Ala Thr Asn Pro Glu Ala Gln Arg Leu Leu Ile Glu Ser Gly Gly 
            260                 265                 270         


Glu Ser Leu Arg Ala Gly Val Arg Asn Met Met Glu Asp Leu Thr Arg 
        275                 280                 285             


Gly Lys Ile Ser Gln Thr Asp Glu Ser Ala Phe Glu Val Gly Arg Asn 
    290                 295                 300                 


Val Ala Val Thr Glu Gly Ala Val Val Phe Glu Asn Glu Tyr Phe Gln 
305                 310                 315                 320 


Leu Leu Gln Tyr Lys Pro Leu Thr Asp Lys Val His Ala Arg Pro Leu 
                325                 330                 335     


Leu Met Val Pro Pro Cys Ile Asn Lys Tyr Tyr Ile Leu Asp Leu Gln 
            340                 345                 350         


Pro Glu Ser Ser Leu Val Arg His Val Val Glu Gln Gly His Thr Val 
        355                 360                 365             


Phe Leu Val Ser Trp Arg Asn Pro Asp Ala Ser Met Ala Gly Ser Thr 
    370                 375                 380                 


Trp Asp Asp Tyr Ile Glu His Ala Ala Ile Arg Ala Ile Glu Val Ala 
385                 390                 395                 400 


Arg Asp Ile Ser Gly Gln Asp Lys Ile Asn Val Leu Gly Phe Cys Val 
                405                 410                 415     


Gly Gly Thr Ile Val Ser Thr Ala Leu Ala Val Leu Ala Ala Arg Gly 
            420                 425                 430         


Glu His Pro Ala Ala Ser Val Thr Leu Leu Thr Thr Leu Leu Asp Phe 
        435                 440                 445             


Ala Asp Thr Gly Ile Leu Asp Val Phe Val Asp Glu Gly His Val Gln 
    450                 455                 460                 


Leu Arg Glu Ala Thr Leu Gly Gly Gly Ala Gly Ala Pro Cys Ala Leu 
465                 470                 475                 480 


Leu Arg Gly Leu Glu Leu Ala Asn Thr Phe Ser Phe Leu Arg Pro Asn 
                485                 490                 495     


Asp Leu Val Trp Asn Tyr Val Val Asp Asn Tyr Leu Lys Gly Asn Thr 
            500                 505                 510         


Pro Val Pro Phe Asp Leu Leu Phe Trp Asn Gly Asp Ala Thr Asn Leu 
        515                 520                 525             


Pro Gly Pro Trp Tyr Cys Trp Tyr Leu Arg His Thr Tyr Leu Gln Asn 
    530                 535                 540                 


Glu Leu Lys Val Pro Gly Lys Leu Thr Val Cys Gly Val Pro Val Asp 
545                 550                 555                 560 


Leu Ala Ser Ile Asp Val Pro Thr Tyr Ile Tyr Gly Ser Arg Glu Asp 
                565                 570                 575     


His Ile Val Pro Trp Thr Ala Ala Tyr Ala Ser Thr Ala Leu Leu Ala 
            580                 585                 590         


Asn Lys Leu Arg Phe Val Leu Gly Ala Ser Gly His Ile Ala Gly Val 
        595                 600                 605             


Ile Asn Pro Pro Ala Lys Asn Lys Arg Ser His Trp Thr Asn Asp Ala 
    610                 615                 620                 


Leu Pro Glu Ser Pro Gln Gln Trp Leu Ala Gly Ala Ile Glu His His 
625                 630                 635                 640 


Gly Ser Trp Trp Pro Asp Trp Thr Ala Trp Leu Ala Gly Gln Ala Gly 
                645                 650                 655     


Ala Lys Arg Ala Ala Pro Ala Asn Tyr Gly Asn Ala Arg Tyr Arg Ala 
            660                 665                 670         


Ile Glu Pro Ala Pro Gly Arg Tyr Val Lys Ala Lys Ala 
        675                 680                 685 


<210>  10
<211>  914
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic fusion polypeptide


<220>
<221>  MISC_FEATURE
<223>  CFP10-RV3615C-PhaC-ESAT6 fusion

<400>  10

Met Ala Glu Met Lys Thr Asp Ala Ala Thr Leu Ala Gln Glu Ala Gly 
1               5                   10                  15      


Asn Phe Glu Arg Ile Ser Gly Asp Leu Lys Thr Gln Ile Asp Gln Val 
            20                  25                  30          


Glu Ser Thr Ala Gly Ser Leu Gln Gly Gln Trp Arg Gly Ala Ala Gly 
        35                  40                  45              


Thr Ala Ala Gln Ala Ala Val Val Arg Phe Gln Glu Ala Ala Asn Lys 
    50                  55                  60                  


Gln Lys Gln Glu Leu Asp Glu Ile Ser Thr Asn Ile Arg Gln Ala Gly 
65                  70                  75                  80  


Val Gln Tyr Ser Arg Ala Asp Glu Glu Gln Gln Gln Ala Leu Ser Ser 
                85                  90                  95      


Gln Met Gly Phe Gly Pro Gly Gly Gly Gly Gly Pro Met Thr Glu Asn 
            100                 105                 110         


Leu Thr Val Gln Pro Glu Arg Leu Gly Val Leu Ala Ser His His Asp 
        115                 120                 125             


Asn Ala Ala Val Asp Ala Ser Ser Gly Val Glu Ala Ala Ala Gly Leu 
    130                 135                 140                 


Gly Glu Ser Val Ala Ile Thr His Gly Pro Tyr Cys Ser Gln Phe Asn 
145                 150                 155                 160 


Asp Thr Leu Asn Val Tyr Leu Thr Ala His Asn Ala Leu Gly Ser Ser 
                165                 170                 175     


Leu His Thr Ala Gly Val Asp Leu Ala Lys Ser Leu Arg Ile Ala Ala 
            180                 185                 190         


Lys Ile Tyr Ser Glu Ala Asp Glu Ala Trp Arg Lys Ala Ile Asp Gly 
        195                 200                 205             


Leu Phe Thr Thr Ser Ala Thr Gly Lys Gly Ala Ala Ala Ser Thr Gln 
    210                 215                 220                 


Glu Gly Lys Ser Gln Pro Phe Lys Val Thr Pro Gly Pro Phe Asp Pro 
225                 230                 235                 240 


Ala Thr Trp Leu Glu Trp Ser Arg Gln Trp Gln Gly Thr Glu Gly Asn 
                245                 250                 255     


Gly His Ala Ala Ala Ser Gly Ile Pro Gly Leu Asp Ala Leu Ala Gly 
            260                 265                 270         


Val Lys Ile Ala Pro Ala Gln Leu Gly Asp Ile Gln Gln Arg Tyr Met 
        275                 280                 285             


Lys Asp Phe Ser Ala Leu Trp Gln Ala Met Ala Glu Gly Lys Ala Glu 
    290                 295                 300                 


Ala Thr Gly Pro Leu His Asp Arg Arg Phe Ala Gly Asp Ala Trp Arg 
305                 310                 315                 320 


Thr Asn Leu Pro Tyr Arg Phe Ala Ala Ala Phe Tyr Leu Leu Asn Ala 
                325                 330                 335     


Arg Ala Leu Thr Glu Leu Ala Asp Ala Val Glu Ala Asp Ala Lys Thr 
            340                 345                 350         


Arg Gln Arg Ile Arg Phe Ala Ile Ser Gln Trp Val Asp Ala Met Ser 
        355                 360                 365             


Pro Ala Asn Phe Leu Ala Thr Asn Pro Glu Ala Gln Arg Leu Leu Ile 
    370                 375                 380                 


Glu Ser Gly Gly Glu Ser Leu Arg Ala Gly Val Arg Asn Met Met Glu 
385                 390                 395                 400 


Asp Leu Thr Arg Gly Lys Ile Ser Gln Thr Asp Glu Ser Ala Phe Glu 
                405                 410                 415     


Val Gly Arg Asn Val Ala Val Thr Glu Gly Ala Val Val Phe Glu Asn 
            420                 425                 430         


Glu Tyr Phe Gln Leu Leu Gln Tyr Lys Pro Leu Thr Asp Lys Val His 
        435                 440                 445             


Ala Arg Pro Leu Leu Met Val Pro Pro Cys Ile Asn Lys Tyr Tyr Ile 
    450                 455                 460                 


Leu Asp Leu Gln Pro Glu Ser Ser Leu Val Arg His Val Val Glu Gln 
465                 470                 475                 480 


Gly His Thr Val Phe Leu Val Ser Trp Arg Asn Pro Asp Ala Ser Met 
                485                 490                 495     


Ala Gly Ser Thr Trp Asp Asp Tyr Ile Glu His Ala Ala Ile Arg Ala 
            500                 505                 510         


Ile Glu Val Ala Arg Asp Ile Ser Gly Gln Asp Lys Ile Asn Val Leu 
        515                 520                 525             


Gly Phe Cys Val Gly Gly Thr Ile Val Ser Thr Ala Leu Ala Val Leu 
    530                 535                 540                 


Ala Ala Arg Gly Glu His Pro Ala Ala Ser Val Thr Leu Leu Thr Thr 
545                 550                 555                 560 


Leu Leu Asp Phe Ala Asp Thr Gly Ile Leu Asp Val Phe Val Asp Glu 
                565                 570                 575     


Gly His Val Gln Leu Arg Glu Ala Thr Leu Gly Gly Gly Ala Gly Ala 
            580                 585                 590         


Pro Cys Ala Leu Leu Arg Gly Leu Glu Leu Ala Asn Thr Phe Ser Phe 
        595                 600                 605             


Leu Arg Pro Asn Asp Leu Val Trp Asn Tyr Val Val Asp Asn Tyr Leu 
    610                 615                 620                 


Lys Gly Asn Thr Pro Val Pro Phe Asp Leu Leu Phe Trp Asn Gly Asp 
625                 630                 635                 640 


Ala Thr Asn Leu Pro Gly Pro Trp Tyr Cys Trp Tyr Leu Arg His Thr 
                645                 650                 655     


Tyr Leu Gln Asn Glu Leu Lys Val Pro Gly Lys Leu Thr Val Cys Gly 
            660                 665                 670         


Val Pro Val Asp Leu Ala Ser Ile Asp Val Pro Thr Tyr Ile Tyr Gly 
        675                 680                 685             


Ser Arg Glu Asp His Ile Val Pro Trp Thr Ala Ala Tyr Ala Ser Thr 
    690                 695                 700                 


Ala Leu Leu Ala Asn Lys Leu Arg Phe Val Leu Gly Ala Ser Gly His 
705                 710                 715                 720 


Ile Ala Gly Val Ile Asn Pro Pro Ala Lys Asn Lys Arg Ser His Trp 
                725                 730                 735     


Thr Asn Asp Ala Leu Pro Glu Ser Pro Gln Gln Trp Leu Ala Gly Ala 
            740                 745                 750         


Ile Glu His His Gly Ser Trp Trp Pro Asp Trp Thr Ala Trp Leu Ala 
        755                 760                 765             


Gly Gln Ala Gly Ala Lys Arg Ala Ala Pro Ala Asn Tyr Gly Asn Ala 
    770                 775                 780                 


Arg Tyr Arg Ala Ile Glu Pro Ala Pro Gly Arg Tyr Val Lys Ala Lys 
785                 790                 795                 800 


Ala His Met Val Leu Ala Val Ala Ile Asp Lys Arg Gly Gly Gly Gly 
                805                 810                 815     


Gly Leu Glu Met Thr Glu Gln Gln Trp Asn Phe Ala Gly Ile Glu Ala 
            820                 825                 830         


Ala Ala Ser Ala Ile Gln Gly Asn Val Thr Ser Ile His Ser Leu Leu 
        835                 840                 845             


Asp Glu Gly Lys Gln Ser Leu Thr Lys Leu Ala Ala Ala Trp Gly Gly 
    850                 855                 860                 


Ser Gly Ser Glu Ala Tyr Gln Gly Val Gln Gln Lys Trp Asp Ala Thr 
865                 870                 875                 880 


Ala Thr Glu Leu Asn Asn Ala Leu Gln Asn Leu Ala Arg Thr Ile Ser 
                885                 890                 895     


Glu Ala Gly Gln Ala Met Ala Ser Thr Glu Gly Asn Val Thr Gly Met 
            900                 905                 910         


Phe Ala 
        


<210>  11
<211>  606
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic sequence encoding ESAT6-Rv3020c fusion polypeptide

<400>  11
ctcgagatga cggaacaaca atggaacttt gctggcatcg aagccgccgc atctgctatt       60

caaggcaatg tgacctctat ccactcgctg ctggatgaag gcaaacagag tctgaccaaa      120

ctggcagcag catggggcgg tagcggctct gaagcctatc aaggtgtgca gcaaaaatgg      180

gacgctaccg cgacggaact gaacaatgcc ctgcagaacc tggcacgtac gatttctgaa      240

gcaggtcaag ctatggcaag cacggaaggc aatgtcacgg gcatgttcgc aggcggcggc      300

ggcggcatga gcctgctgga tgcgcatatt ccgcagctga ttgcgagcca taccgcgttt      360

gcggcgaaag cgggcctgat gcgtcatacc attggccagg cggaacagca ggcgatgagc      420

gcgcaggcgt ttcatcaggg cgaaagcgcg gcggcgtttc agggcgcgca tgcgcgtttt      480

gtggcggcgg cggcgaaagt gaacaccctg ctggatattg cgcaggcgaa cctgggcgaa      540

gcggcgggca cctatgtggc ggcggatgcg gcggcggcga gcagctatac cggcttttaa      600

ggatcc                                                                 606


<210>  12
<211>  2073
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic sequence encoding CFP10-PhaC fusion polypeptide

<400>  12
atggcagaaa tgaaaacgga tgcggcgacc ctggcacaag aagcgggcaa ctttgaacgt       60

attagcggcg acctgaaaac ccaaatcgat caggttgaaa gcaccgcggg ttctctgcaa      120

ggccagtggc gtggtgcggc cggtacggca gctcaagcgg ccgtggttcg ctttcaggaa      180

gcagctaaca aacaaaaaca ggaactggat gaaatttcaa ccaatatccg tcaagccggc      240

gtgcagtatt cgcgcgcaga cgaagaacag caacaggctc tgagctctca aatgggtttc      300

catatggcga ccggcaaagg cgcggcagct tccacgcagg aaggcaagtc ccaaccattc      360

aaggtcacgc cggggccatt cgatccagcc acatggctgg aatggtcccg ccagtggcag      420

ggcactgaag gcaacggcca cgcggccgcg tccggcattc cgggcctgga tgcgctggca      480

ggcgtcaaga tcgcgccggc gcagctgggt gatatccagc agcgctacat gaaggacttc      540

tcagcgctgt ggcaggccat ggccgagggc aaggccgagg ccaccggtcc gctgcacgac      600

cggcgcttcg ccggcgacgc atggcgcacc aacctcccat atcgcttcgc tgccgcgttc      660

tacctgctca atgcgcgcgc cttgaccgag ctggccgatg ccgtcgaggc cgatgccaag      720

acccgccagc gcatccgctt cgcgatctcg caatgggtcg atgcgatgtc gcccgccaac      780

ttccttgcca ccaatcccga ggcgcagcgc ctgctgatcg agtcgggcgg cgaatcgctg      840

cgtgccggcg tgcgcaacat gatggaagac ctgacacgcg gcaagatctc gcagaccgac      900

gagagcgcgt ttgaggtcgg ccgcaatgtc gcggtgaccg aaggcgccgt ggtcttcgag      960

aacgagtact tccagctgtt gcagtacaag ccgctgaccg acaaggtgca cgcgcgcccg     1020

ctgctgatgg tgccgccgtg catcaacaag tactacatcc tggacctgca gccggagagc     1080

tcgctggtgc gccatgtggt ggagcaggga catacggtgt ttctggtgtc gtggcgcaat     1140

ccggacgcca gcatggccgg cagcacctgg gacgactaca tcgagcacgc ggccatccgc     1200

gccatcgaag tcgcgcgcga catcagcggc caggacaaga tcaacgtgct cggcttctgc     1260

gtgggcggca ccattgtctc gaccgcgctg gcggtgctgg ccgcgcgcgg cgagcacccg     1320

gccgccagcg tcacgctgct gaccacgctg ctggactttg ccgacacggg catcctcgac     1380

gtctttgtcg acgagggcca tgtgcagttg cgcgaggcca cgctgggcgg cggcgccggc     1440

gcgccgtgcg cgctgctgcg cggccttgag ctggccaata ccttctcgtt cttgcgcccg     1500

aacgacctgg tgtggaacta cgtggtcgac aactacctga agggcaacac gccggtgccg     1560

ttcgacctgc tgttctggaa cggcgacgcc accaacctgc cggggccgtg gtactgctgg     1620

tacctgcgcc acacctacct gcagaacgag ctcaaggtac cgggcaagct gaccgtgtgc     1680

ggcgtgccgg tggacctggc cagcatcgac gtgccgacct atatctacgg ctcgcgcgaa     1740

gaccatatcg tgccgtggac cgcggcctat gcctcgaccg cgctgctggc gaacaagctg     1800

cgcttcgtgc tgggtgcgtc gggccatatc gccggtgtga tcaacccgcc ggccaagaac     1860

aagcgcagcc actggactaa cgatgcgctg ccggagtcgc cgcagcaatg gctggccggc     1920

gccatcgagc atcacggcag ctggtggccg gactggaccg catggctggc cgggcaggcc     1980

ggcgcgaaac gcgccgcgcc cgccaactat ggcaatgcgc gctatcgcgc aatcgaaccc     2040

gcgcctgggc gatacgtcaa agccaaggca tga                                  2073


<210>  13
<211>  2085
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic sequence encoding Rv3615c-PhaC fusion polypeptide

<400>  13
atgatgaccg aaaacctgac ggttcagccg gaacgtctgg gtgtcctggc aagtcatcac       60

gataatgcag cagtcgacgc cagttccggt gtggaagcag ctgcaggtct gggtgaaagt      120

gtggcgatta cccatggtcc gtattgctcc cagtttaacg ataccctgaa tgtttacctg      180

acggcccata acgcactggg ttcatcgctg cacacggctg gtgtcgacct ggcaaaatct      240

ctgcgcatcg ccgcaaaaat ctattcagaa gcagacgaag cgtggcgtaa agcaatcgac      300

ggcctgttca cccatatggc gaccggcaaa ggcgcggcag cttccacgca ggaaggcaag      360

tcccaaccat tcaaggtcac gccggggcca ttcgatccag ccacatggct ggaatggtcc      420

cgccagtggc agggcactga aggcaacggc cacgcggccg cgtccggcat tccgggcctg      480

gatgcgctgg caggcgtcaa gatcgcgccg gcgcagctgg gtgatatcca gcagcgctac      540

atgaaggact tctcagcgct gtggcaggcc atggccgagg gcaaggccga ggccaccggt      600

ccgctgcacg accggcgctt cgccggcgac gcatggcgca ccaacctccc atatcgcttc      660

gctgccgcgt tctacctgct caatgcgcgc gccttgaccg agctggccga tgccgtcgag      720

gccgatgcca agacccgcca gcgcatccgc ttcgcgatct cgcaatgggt cgatgcgatg      780

tcgcccgcca acttccttgc caccaatccc gaggcgcagc gcctgctgat cgagtcgggc      840

ggcgaatcgc tgcgtgccgg cgtgcgcaac atgatggaag acctgacacg cggcaagatc      900

tcgcagaccg acgagagcgc gtttgaggtc ggccgcaatg tcgcggtgac cgaaggcgcc      960

gtggtcttcg agaacgagta cttccagctg ttgcagtaca agccgctgac cgacaaggtg     1020

cacgcgcgcc cgctgctgat ggtgccgccg tgcatcaaca agtactacat cctggacctg     1080

cagccggaga gctcgctggt gcgccatgtg gtggagcagg gacatacggt gtttctggtg     1140

tcgtggcgca atccggacgc cagcatggcc ggcagcacct gggacgacta catcgagcac     1200

gcggccatcc gcgccatcga agtcgcgcgc gacatcagcg gccaggacaa gatcaacgtg     1260

ctcggcttct gcgtgggcgg caccattgtc tcgaccgcgc tggcggtgct ggccgcgcgc     1320

ggcgagcacc cggccgccag cgtcacgctg ctgaccacgc tgctggactt tgccgacacg     1380

ggcatcctcg acgtctttgt cgacgagggc catgtgcagt tgcgcgaggc cacgctgggc     1440

ggcggcgccg gcgcgccgtg cgcgctgctg cgcggccttg agctggccaa taccttctcg     1500

ttcttgcgcc cgaacgacct ggtgtggaac tacgtggtcg acaactacct gaagggcaac     1560

acgccggtgc cgttcgacct gctgttctgg aacggcgacg ccaccaacct gccggggccg     1620

tggtactgct ggtacctgcg ccacacctac ctgcagaacg agctcaaggt accgggcaag     1680

ctgaccgtgt gcggcgtgcc ggtggacctg gccagcatcg acgtgccgac ctatatctac     1740

ggctcgcgcg aagaccatat cgtgccgtgg accgcggcct atgcctcgac cgcgctgctg     1800

gcgaacaagc tgcgcttcgt gctgggtgcg tcgggccata tcgccggtgt gatcaacccg     1860

ccggccaaga acaagcgcag ccactggact aacgatgcgc tgccggagtc gccgcagcaa     1920

tggctggccg gcgccatcga gcatcacggc agctggtggc cggactggac cgcatggctg     1980

gccgggcagg ccggcgcgaa acgcgccgcg cccgccaact atggcaatgc gcgctatcgc     2040

gcaatcgaac ccgcgcctgg gcgatacgtc aaagccaagg catga                     2085


<210>  14
<211>  2058
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic sequence encoding ESAT6-PhaC fusion polypeptide

<400>  14
atgacggaac aacaatggaa ctttgctggc atcgaagccg ccgcatctgc tattcaaggc       60

aatgtgacct ctatccactc gctgctggat gaaggcaaac agagtctgac caaactggca      120

gcagcatggg gcggtagcgg ctctgaagcc tatcaaggtg tgcagcaaaa atgggacgct      180

accgcgacgg aactgaacaa tgccctgcag aacctggcac gtacgatttc tgaagcaggt      240

caagctatgg caagcacgga aggcaatgtc acgggcatgt tcgcacatat ggcgaccggc      300

aaaggcgcgg cagcttccac gcaggaaggc aagtcccaac cattcaaggt cacgccgggg      360

ccattcgatc cagccacatg gctggaatgg tcccgccagt ggcagggcac tgaaggcaac      420

ggccacgcgg ccgcgtccgg cattccgggc ctggatgcgc tggcaggcgt caagatcgcg      480

ccggcgcagc tgggtgatat ccagcagcgc tacatgaagg acttctcagc gctgtggcag      540

gccatggccg agggcaaggc cgaggccacc ggtccgctgc acgaccggcg cttcgccggc      600

gacgcatggc gcaccaacct cccatatcgc ttcgctgccg cgttctacct gctcaatgcg      660

cgcgccttga ccgagctggc cgatgccgtc gaggccgatg ccaagacccg ccagcgcatc      720

cgcttcgcga tctcgcaatg ggtcgatgcg atgtcgcccg ccaacttcct tgccaccaat      780

cccgaggcgc agcgcctgct gatcgagtcg ggcggcgaat cgctgcgtgc cggcgtgcgc      840

aacatgatgg aagacctgac acgcggcaag atctcgcaga ccgacgagag cgcgtttgag      900

gtcggccgca atgtcgcggt gaccgaaggc gccgtggtct tcgagaacga gtacttccag      960

ctgttgcagt acaagccgct gaccgacaag gtgcacgcgc gcccgctgct gatggtgccg     1020

ccgtgcatca acaagtacta catcctggac ctgcagccgg agagctcgct ggtgcgccat     1080

gtggtggagc agggacatac ggtgtttctg gtgtcgtggc gcaatccgga cgccagcatg     1140

gccggcagca cctgggacga ctacatcgag cacgcggcca tccgcgccat cgaagtcgcg     1200

cgcgacatca gcggccagga caagatcaac gtgctcggct tctgcgtggg cggcaccatt     1260

gtctcgaccg cgctggcggt gctggccgcg cgcggcgagc acccggccgc cagcgtcacg     1320

ctgctgacca cgctgctgga ctttgccgac acgggcatcc tcgacgtctt tgtcgacgag     1380

ggccatgtgc agttgcgcga ggccacgctg ggcggcggcg ccggcgcgcc gtgcgcgctg     1440

ctgcgcggcc ttgagctggc caataccttc tcgttcttgc gcccgaacga cctggtgtgg     1500

aactacgtgg tcgacaacta cctgaagggc aacacgccgg tgccgttcga cctgctgttc     1560

tggaacggcg acgccaccaa cctgccgggg ccgtggtact gctggtacct gcgccacacc     1620

tacctgcaga acgagctcaa ggtaccgggc aagctgaccg tgtgcggcgt gccggtggac     1680

ctggccagca tcgacgtgcc gacctatatc tacggctcgc gcgaagacca tatcgtgccg     1740

tggaccgcgg cctatgcctc gaccgcgctg ctggcgaaca agctgcgctt cgtgctgggt     1800

gcgtcgggcc atatcgccgg tgtgatcaac ccgccggcca agaacaagcg cagccactgg     1860

actaacgatg cgctgccgga gtcgccgcag caatggctgg ccggcgccat cgagcatcac     1920

ggcagctggt ggccggactg gaccgcatgg ctggccgggc aggccggcgc gaaacgcgcc     1980

gcgcccgcca actatggcaa tgcgcgctat cgcgcaatcg aacccgcgcc tgggcgatac     2040

gtcaaagcca aggcatga                                                   2058


<210>  15
<211>  2745
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic sequence encoding CFP10-Rv3615c-PhaC-ESAT6 fusion 
       polypeptide

<400>  15
atggcagaaa tgaaaacgga tgcggcgacc ctggcacaag aagcgggcaa ctttgaacgt       60

attagcggcg acctgaaaac ccaaatcgat caggttgaaa gcaccgcggg ttctctgcaa      120

ggccagtggc gtggtgcggc cggtacggca gctcaagcgg ccgtggttcg ctttcaggaa      180

gcagctaaca aacaaaaaca ggaactggat gaaatttcaa ccaatatccg tcaagccggc      240

gtgcagtatt cgcgcgcaga cgaagaacag caacaggctc tgagctctca aatgggtttc      300

gggcccggcg gtggcggtgg cccgatgacc gaaaacctga cggttcagcc ggaacgtctg      360

ggtgtcctgg caagtcatca cgataatgca gcagtcgacg ccagttccgg tgtggaagca      420

gctgcaggtc tgggtgaaag tgtggcgatt acccatggtc cgtattgctc ccagtttaac      480

gataccctga atgtttacct gacggcccat aacgcactgg gttcatcgct gcacacggct      540

ggtgtcgacc tggcaaaatc tctgcgcatc gccgcaaaaa tctattcaga agcagacgaa      600

gcgtggcgta aagcaatcga cggcctgttc accactagtg cgaccggcaa aggcgcggca      660

gcttccacgc aggaaggcaa gtcccaacca ttcaaggtca cgccggggcc attcgatcca      720

gccacatggc tggaatggtc ccgccagtgg cagggcactg aaggcaacgg ccacgcggcc      780

gcgtccggca ttccgggcct ggatgcgctg gcaggcgtca agatcgcgcc ggcgcagctg      840

ggtgatatcc agcagcgcta catgaaggac ttctcagcgc tgtggcaggc catggccgag      900

ggcaaggccg aggccaccgg tccgctgcac gaccggcgct tcgccggcga cgcatggcgc      960

accaacctcc catatcgctt cgctgccgcg ttctacctgc tcaatgcgcg cgccttgacc     1020

gagctggccg atgccgtcga ggccgatgcc aagacccgcc agcgcatccg cttcgcgatc     1080

tcgcaatggg tcgatgcgat gtcgcccgcc aacttccttg ccaccaatcc cgaggcgcag     1140

cgcctgctga tcgagtcggg cggcgaatcg ctgcgtgccg gcgtgcgcaa catgatggaa     1200

gacctgacac gcggcaagat ctcgcagacc gacgagagcg cgtttgaggt cggccgcaat     1260

gtcgcggtga ccgaaggcgc cgtggtcttc gagaacgagt acttccagct gttgcagtac     1320

aagccgctga ccgacaaggt gcacgcgcgc ccgctgctga tggtgccgcc gtgcatcaac     1380

aagtactaca tcctggacct gcagccggag agctcgctgg tgcgccatgt ggtggagcag     1440

ggacatacgg tgtttctggt gtcgtggcgc aatccggacg ccagcatggc cggcagcacc     1500

tgggacgact acatcgagca cgcggccatc cgcgccatcg aagtcgcgcg cgacatcagc     1560

ggccaggaca agatcaacgt gctcggcttc tgcgtgggcg gcaccattgt ctcgaccgcg     1620

ctggcggtgc tggccgcgcg cggcgagcac ccggccgcca gcgtcacgct gctgaccacg     1680

ctgctggact ttgccgacac gggcatcctc gacgtctttg tcgacgaggg ccatgtgcag     1740

ttgcgcgagg ccacgctggg cggcggcgcc ggcgcgccgt gcgcgctgct gcgcggcctt     1800

gagctggcca ataccttctc gttcttgcgc ccgaacgacc tggtgtggaa ctacgtggtc     1860

gacaactacc tgaagggcaa cacgccggtg ccgttcgacc tgctgttctg gaacggcgac     1920

gccaccaacc tgccggggcc gtggtactgc tggtacctgc gccacaccta cctgcagaac     1980

gagctcaagg taccgggcaa gctgaccgtg tgcggcgtgc cggtggacct ggccagcatc     2040

gacgtgccga cctatatcta cggctcgcgc gaagaccata tcgtgccgtg gaccgcggcc     2100

tatgcctcga ccgcgctgct ggcgaacaag ctgcgcttcg tgctgggtgc gtcgggccat     2160

atcgccggtg tgatcaaccc gccggccaag aacaagcgca gccactggac taacgatgcg     2220

ctgccggagt cgccgcagca atggctggcc ggcgccatcg agcatcacgg cagctggtgg     2280

ccggactgga ccgcatggct ggccgggcag gccggcgcga aacgcgccgc gcccgccaac     2340

tatggcaatg cgcgctatcg cgcaatcgaa cccgcgcctg ggcgatacgt caaagccaag     2400

gcacatatgg tgctggcggt ggcgattgat aaacgcggag gcggtggagg cctcgagatg     2460

acggaacaac aatggaactt tgctggcatc gaagccgccg catctgctat tcaaggcaat     2520

gtgacctcta tccactcgct gctggatgaa ggcaaacaga gtctgaccaa actggcagca     2580

gcatggggcg gtagcggctc tgaagcctat caaggtgtgc agcaaaaatg ggacgctacc     2640

gcgacggaac tgaacaatgc cctgcagaac ctggcacgta cgatttctga agcaggtcaa     2700

gctatggcaa gcacggaagg caatgtcacg ggcatgttcg cataa                     2745


<210>  16
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide

<400>  16
aacatatggc agaaatgaaa acggatgcgg c                                      31


<210>  17
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide

<400>  17
ttcatatgga aacccatttg agagctcaga gcc                                    33


<210>  18
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide

<400>  18
aacatatgac cgaaaacctg acggttcagc cgg                                    33


<210>  19
<211>  34
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide

<400>  19
ttcatatggg tgaacaggcc gtcgattgct ttac                                   34


<210>  20
<211>  35
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide

<400>  20
aacatatgac ggaacaacaa tggaactttg ctggc                                  35


<210>  21
<211>  35
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide

<400>  21
ttcatatgtg cgaacatgcc cgtgacattg ccttc                                  35


<210>  22
<211>  1016
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic fusion polypeptide


<220>
<221>  MISC_FEATURE
<223>  CFP10-Rv3615c-PhaC-ESAT6-Rv3020c fusion

<400>  22

Met Ala Glu Met Lys Thr Asp Ala Ala Thr Leu Ala Gln Glu Ala Gly 
1               5                   10                  15      


Asn Phe Glu Arg Ile Ser Gly Asp Leu Lys Thr Gln Ile Asp Gln Val 
            20                  25                  30          


Glu Ser Thr Ala Gly Ser Leu Gln Gly Gln Trp Arg Gly Ala Ala Gly 
        35                  40                  45              


Thr Ala Ala Gln Ala Ala Val Val Arg Phe Gln Glu Ala Ala Asn Lys 
    50                  55                  60                  


Gln Lys Gln Glu Leu Asp Glu Ile Ser Thr Asn Ile Arg Gln Ala Gly 
65                  70                  75                  80  


Val Gln Tyr Ser Arg Ala Asp Glu Glu Gln Gln Gln Ala Leu Ser Ser 
                85                  90                  95      


Gln Met Gly Phe Gly Pro Gly Gly Gly Gly Gly Pro Met Thr Glu Asn 
            100                 105                 110         


Leu Thr Val Gln Pro Glu Arg Leu Gly Val Leu Ala Ser His His Asp 
        115                 120                 125             


Asn Ala Ala Val Asp Ala Ser Ser Gly Val Glu Ala Ala Ala Gly Leu 
    130                 135                 140                 


Gly Glu Ser Val Ala Ile Thr His Gly Pro Tyr Cys Ser Gln Phe Asn 
145                 150                 155                 160 


Asp Thr Leu Asn Val Tyr Leu Thr Ala His Asn Ala Leu Gly Ser Ser 
                165                 170                 175     


Leu His Thr Ala Gly Val Asp Leu Ala Lys Ser Leu Arg Ile Ala Ala 
            180                 185                 190         


Lys Ile Tyr Ser Glu Ala Asp Glu Ala Trp Arg Lys Ala Ile Asp Gly 
        195                 200                 205             


Leu Phe Thr Thr Ser Ala Thr Gly Lys Gly Ala Ala Ala Ser Thr Gln 
    210                 215                 220                 


Glu Gly Lys Ser Gln Pro Phe Lys Val Thr Pro Gly Pro Phe Asp Pro 
225                 230                 235                 240 


Ala Thr Trp Leu Glu Trp Ser Arg Gln Trp Gln Gly Thr Glu Gly Asn 
                245                 250                 255     


Gly His Ala Ala Ala Ser Gly Ile Pro Gly Leu Asp Ala Leu Ala Gly 
            260                 265                 270         


Val Lys Ile Ala Pro Ala Gln Leu Gly Asp Ile Gln Gln Arg Tyr Met 
        275                 280                 285             


Lys Asp Phe Ser Ala Leu Trp Gln Ala Met Ala Glu Gly Lys Ala Glu 
    290                 295                 300                 


Ala Thr Gly Pro Leu His Asp Arg Arg Phe Ala Gly Asp Ala Trp Arg 
305                 310                 315                 320 


Thr Asn Leu Pro Tyr Arg Phe Ala Ala Ala Phe Tyr Leu Leu Asn Ala 
                325                 330                 335     


Arg Ala Leu Thr Glu Leu Ala Asp Ala Val Glu Ala Asp Ala Lys Thr 
            340                 345                 350         


Arg Gln Arg Ile Arg Phe Ala Ile Ser Gln Trp Val Asp Ala Met Ser 
        355                 360                 365             


Pro Ala Asn Phe Leu Ala Thr Asn Pro Glu Ala Gln Arg Leu Leu Ile 
    370                 375                 380                 


Glu Ser Gly Gly Glu Ser Leu Arg Ala Gly Val Arg Asn Met Met Glu 
385                 390                 395                 400 


Asp Leu Thr Arg Gly Lys Ile Ser Gln Thr Asp Glu Ser Ala Phe Glu 
                405                 410                 415     


Val Gly Arg Asn Val Ala Val Thr Glu Gly Ala Val Val Phe Glu Asn 
            420                 425                 430         


Glu Tyr Phe Gln Leu Leu Gln Tyr Lys Pro Leu Thr Asp Lys Val His 
        435                 440                 445             


Ala Arg Pro Leu Leu Met Val Pro Pro Cys Ile Asn Lys Tyr Tyr Ile 
    450                 455                 460                 


Leu Asp Leu Gln Pro Glu Ser Ser Leu Val Arg His Val Val Glu Gln 
465                 470                 475                 480 


Gly His Thr Val Phe Leu Val Ser Trp Arg Asn Pro Asp Ala Ser Met 
                485                 490                 495     


Ala Gly Ser Thr Trp Asp Asp Tyr Ile Glu His Ala Ala Ile Arg Ala 
            500                 505                 510         


Ile Glu Val Ala Arg Asp Ile Ser Gly Gln Asp Lys Ile Asn Val Leu 
        515                 520                 525             


Gly Phe Cys Val Gly Gly Thr Ile Val Ser Thr Ala Leu Ala Val Leu 
    530                 535                 540                 


Ala Ala Arg Gly Glu His Pro Ala Ala Ser Val Thr Leu Leu Thr Thr 
545                 550                 555                 560 


Leu Leu Asp Phe Ala Asp Thr Gly Ile Leu Asp Val Phe Val Asp Glu 
                565                 570                 575     


Gly His Val Gln Leu Arg Glu Ala Thr Leu Gly Gly Gly Ala Gly Ala 
            580                 585                 590         


Pro Cys Ala Leu Leu Arg Gly Leu Glu Leu Ala Asn Thr Phe Ser Phe 
        595                 600                 605             


Leu Arg Pro Asn Asp Leu Val Trp Asn Tyr Val Val Asp Asn Tyr Leu 
    610                 615                 620                 


Lys Gly Asn Thr Pro Val Pro Phe Asp Leu Leu Phe Trp Asn Gly Asp 
625                 630                 635                 640 


Ala Thr Asn Leu Pro Gly Pro Trp Tyr Cys Trp Tyr Leu Arg His Thr 
                645                 650                 655     


Tyr Leu Gln Asn Glu Leu Lys Val Pro Gly Lys Leu Thr Val Cys Gly 
            660                 665                 670         


Val Pro Val Asp Leu Ala Ser Ile Asp Val Pro Thr Tyr Ile Tyr Gly 
        675                 680                 685             


Ser Arg Glu Asp His Ile Val Pro Trp Thr Ala Ala Tyr Ala Ser Thr 
    690                 695                 700                 


Ala Leu Leu Ala Asn Lys Leu Arg Phe Val Leu Gly Ala Ser Gly His 
705                 710                 715                 720 


Ile Ala Gly Val Ile Asn Pro Pro Ala Lys Asn Lys Arg Ser His Trp 
                725                 730                 735     


Thr Asn Asp Ala Leu Pro Glu Ser Pro Gln Gln Trp Leu Ala Gly Ala 
            740                 745                 750         


Ile Glu His His Gly Ser Trp Trp Pro Asp Trp Thr Ala Trp Leu Ala 
        755                 760                 765             


Gly Gln Ala Gly Ala Lys Arg Ala Ala Pro Ala Asn Tyr Gly Asn Ala 
    770                 775                 780                 


Arg Tyr Arg Ala Ile Glu Pro Ala Pro Gly Arg Tyr Val Lys Ala Lys 
785                 790                 795                 800 


Ala His Met Val Leu Ala Val Ala Ile Asp Lys Arg Gly Gly Gly Gly 
                805                 810                 815     


Gly Leu Glu Met Thr Glu Gln Gln Trp Asn Phe Ala Gly Ile Glu Ala 
            820                 825                 830         


Ala Ala Ser Ala Ile Gln Gly Asn Val Thr Ser Ile His Ser Leu Leu 
        835                 840                 845             


Asp Glu Gly Lys Gln Ser Leu Thr Lys Leu Ala Ala Ala Trp Gly Gly 
    850                 855                 860                 


Ser Gly Ser Glu Ala Tyr Gln Gly Val Gln Gln Lys Trp Asp Ala Thr 
865                 870                 875                 880 


Ala Thr Glu Leu Asn Asn Ala Leu Gln Asn Leu Ala Arg Thr Ile Ser 
                885                 890                 895     


Glu Ala Gly Gln Ala Met Ala Ser Thr Glu Gly Asn Val Thr Gly Met 
            900                 905                 910         


Phe Ala Gly Gly Gly Gly Gly Met Ser Leu Leu Asp Ala His Ile Pro 
        915                 920                 925             


Gln Leu Ile Ala Ser His Thr Ala Phe Ala Ala Lys Ala Gly Leu Met 
    930                 935                 940                 


Arg His Thr Ile Gly Gln Ala Glu Gln Gln Ala Met Ser Ala Gln Ala 
945                 950                 955                 960 


Phe His Gln Gly Glu Ser Ala Ala Ala Phe Gln Gly Ala His Ala Arg 
                965                 970                 975     


Phe Val Ala Ala Ala Ala Lys Val Asn Thr Leu Leu Asp Ile Ala Gln 
            980                 985                 990         


Ala Asn Leu Gly Glu Ala Ala Gly  Thr Tyr Val Ala Ala  Asp Ala Ala 
        995                 1000                 1005             


Ala Ala  Ser Ser Tyr Thr Gly  Phe 
    1010                 1015     


