                         SEQUENCE LISTING

<110>  Institut d'Investigacions Biomdiques August Pi i Sunyer 
       (IDIBAPS)
 
<120>  Conditionally replicating adenovirus and use thereof in the 
       treatment of pancreatic cancer

<130>  P2902ES00

<150>   ES 201430147
<151>  2013-02-06

<160>  35    

<170>  PatentIn version 3.5

<210>  1
<211>  22
<212>  RNA
<213>  Homo sapiens

<400>  1
ucagugcacu acagaacuuu gu                                                22


<210>  2
<211>  6
<212>  RNA
<213>  Homo sapiens

<400>  2
agugca                                                                   6


<210>  3
<211>  7
<212>  RNA
<213>  Homo sapiens

<400>  3
cagugca                                                                  7


<210>  4
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence which hybridizes with miR-148a

<400>  4
gcactg                                                                   6


<210>  5
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence which hybrizes with miR-148a

<400>  5
gtgcactg                                                                 8


<210>  6
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence which hybridizes with miR-148a

<400>  6
acaaagttct gtagtgcact ga                                                22


<210>  7
<211>  4
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  spacer

<400>  7
atgc                                                                     4


<210>  8
<211>  4
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  spacer

<400>  8
tgca                                                                     4


<210>  9
<211>  4
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  spacer

<400>  9
gcat                                                                     4


<210>  10
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  spacer

<400>  10
gataacacta gagataac                                                     18


<210>  11
<211>  221
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  heterologous sequence included in the 3'-UTR region of the 
       adenovirus, comprising 8 copies of sequence SEQ ID NO: 6, each 
       copy separated by a spacer sequence

<400>  11
acaaagttct gtagtgcact gaatgcacaa agttctgtag tgcactgatg caacaaagtt       60

ctgtagtgca ctgagcatac aaagttctgt agtgcactga gataacacta gagataacac      120

aaagttctgt agtgcactga atgcacaaag ttctgtagtg cactgatgca acaaagttct      180

gtagtgcact gagcatacaa agttctgtag tgcactgagt t                          221


<210>  12
<211>  22
<212>  RNA
<213>  Homo sapiens

<400>  12
uaaucucagc uggcaacugu ga                                                22


<210>  13
<211>  6
<212>  RNA
<213>  Homo sapiens

<400>  13
aaucuc                                                                   6


<210>  14
<211>  7
<212>  RNA
<213>  Homo sapiens

<400>  14
aaucuca                                                                  7


<210>  15
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence which hybridizes with miR-216a

<400>  15
gagatt                                                                   6


<210>  16
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence which hybridizes with miR-216a

<400>  16
ctgagatt                                                                 8


<210>  17
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence which hybridizes with miR216a

<400>  17
tcacagttgc cagctgagat ta                                                22


<210>  18
<211>  221
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  heterologous sequence included in the 3'-UTR of the adenovirus, 
       comprising 4 copies of SEQ ID NO: 6 and 4 copies of SEQ ID NO: 
       17, the copies being separated by spacer sequences

<400>  18
acaaagttct gtagtgcact gaatgcacaa agttctgtag tgcactgatg caacaaagtt       60

ctgtagtgca ctgagcatac aaagttctgt agtgcactga gataacacta gagataactc      120

acagttgcca gctgagatta atgctcacag ttgccagctg agattatgca tcacagttgc      180

cagctgagat tagcattcac agttgccagc tgagattagt t                          221


<210>  19
<211>  118
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide comprising 4 fully complementary target sites for
       miR-148a separated by three spacer sequences , a HpaI restriction
       site at 5' and 3'  extreme plus the restriction site ends for 
       XbaI.

<400>  19
ctagagttaa cacaaagttc tgtagtgcac tgaatgcaca aagttctgta gtgcactgat       60

gcaacaaagt tctgtagtgc actgagcata caaagttctg tagtgcactg agttaact        118


<210>  20
<211>  118
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide comprising 4 fully complementary target sites for
       miR-148a separated by three spacer sequences , a HpaI restriction
       site at 5' and 3'  extreme plus the restriction site ends for 
       XbaI.

<400>  20
ctagagttaa ctcagtgcac tacagaactt tgtatgctca gtgcactaca gaactttgtt       60

gcatcagtgc actacagaac tttgtgcatt cagtgcacta cagaactttg tgttaact        118


<210>  21
<211>  118
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide comprising 4 fully complementary target sites for
       miR-216a separated by three spacer sequences , a HpaI restriction
       site at 5' and 3'  extreme plus the restriction site ends for 
       XbaI.

<400>  21
ctagagttaa ctcacagttg ccagctgaga ttaatgctca cagttgccag ctgagattat       60

gcatcacagt tgccagctga gattagcatt cacagttgcc agctgagatt agttaact        118


<210>  22
<211>  118
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide comprising 4 fully complementary target sites for
       miR-216a separated by three spacer sequences , a HpaI restriction
       site at 5' and 3'  extreme plus the restriction site ends for 
       XbaI.

<400>  22
ctagagttaa ctaatctcag ctggcaactg tgaatgctaa tctcagctgg caactgtgat       60

gcataatctc agctggcaac tgtgagcatt aatctcagct ggcaactgtg agttaact        118


<210>  23
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  23
aaagttctgt agtgcactga gataacacta gagataacac aaagttctgt agtgc            55


<210>  24
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  24
gttctgtagt gcactgagat aacactagag ataactcaca gttgccagc                   49


<210>  25
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  25
gggaagcttg ttccattatc ggtcgcatc                                         29


<210>  26
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  26
cccaagcttt tggtcaagtt cctcgtgct                                         29


<210>  27
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  27
gggaagcttg cgaatccaat aaaggcaaa                                         29


<210>  28
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  28
cccaagctta ggggtacatc agggcttct                                         29


<210>  29
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  29
ccacagcctc tagagacaaa gttctgtagg cgtgcaactt ctatcatact cagagtcgga       60

gtgtc                                                                   65


<210>  30
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  30
gccgcagtgg tcttacatgc acatc                                             25


<210>  31
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  31
cagcacgccg cggatgtcaa ag                                                22


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  32
cggccatttc ttcggtaata                                                   20


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  33
cctccggtga taatgacaag                                                   20


<210>  34
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  34
ggcagctgat ctccaaagtc ctgg                                              24


<210>  35
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  35
aacgttcgat gtcatccagt gtta                                              24


