                         SEQUENCE LISTING

<110>  F. Hoffmann-La Roche AG
 
<120>  Interleukin-2 fusion proteins and uses thereof

<130>  31142

<150>  US 61/936564
<151>  2014-02-06

<160>  66    

<170>  PatentIn version 3.5

<210>  1
<211>  133
<212>  PRT
<213>  Homo sapiens

<400>  1

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  2
<211>  399
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wild-type human IL-2

<400>  2
gcacctactt caagttctac aaagaaaaca cagctacaac tggagcattt actgctggat       60

ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc      120

acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa      180

gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta      240

agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa      300

acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga      360

tggattacct tttgtcaaag catcatctca acactgact                             399


<210>  3
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Wild-type human IL-2 (C125A)

<400>  3

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ala Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  4
<211>  399
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wild-type human IL-2 (C125A) (1)

<400>  4
gctcctacat cctccagcac caagaaaacc cagctccagc tggaacatct cctgctggat       60

ctgcagatga tcctgaacgg catcaacaac tacaagaacc ccaagctgac ccggatgctg      120

accttcaagt tctacatgcc caagaaggcc accgagctga aacatctgca gtgcctggaa      180

gaggaactga agcctctgga agaggtgctg aacctggccc agtccaagaa cttccacctg      240

aggcctcggg acctgatctc caacatcaac gtgatcgtgc tggaactgaa gggctccgag      300

acaaccttca tgtgcgagta cgccgacgag acagctacca tcgtggaatt tctgaaccgg      360

tggatcacct tcgcccagtc catcatctcc accctgacc                             399


<210>  5
<211>  399
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wild-type human IL-2 (C125A) (2)

<400>  5
gcacctactt caagttctac aaagaaaaca cagctacaac tggagcattt actgctggat       60

ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc      120

acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa      180

gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta      240

agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa      300

acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga      360

tggattacct ttgcccaaag catcatctca acactgact                             399


<210>  6
<211>  399
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wild-type human IL-2 (C125A) (3)

<400>  6
gctcctacta gcagctccac caagaaaacc cagctccagc tggaacatct gctgctggat       60

ctgcagatga tcctgaacgg catcaacaac tacaagaacc ccaagctgac ccggatgctg      120

accttcaagt tctacatgcc caagaaggcc accgaactga aacatctgca gtgcctggaa      180

gaggaactga agcctctgga agaggtgctg aacctggccc agagcaagaa cttccacctg      240

aggcccaggg acctgatcag caacatcaac gtgatcgtgc tggaactgaa gggcagcgag      300

acaaccttca tgtgcgagta cgccgacgag acagccacca tcgtggaatt tctgaaccgg      360

tggatcacct tcgcccagag catcatcagc accctgaca                             399


<210>  7
<211>  399
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wild-type IL-2 (C125A) (4)

<400>  7
gcacctactt caagttctac aaagaaaaca cagctacaac tggagcattt actgctggat       60

ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc      120

acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa      180

gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta      240

agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa      300

acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga      360

tggattacct ttgcccaaag catcatctca acactgact                             399


<210>  8
<211>  227
<212>  PRT
<213>  Homo sapiens

<400>  8

Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
1               5                   10                  15      


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            20                  25                  30          


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        35                  40                  45              


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    50                  55                  60                  


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
65                  70                  75                  80  


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                85                  90                  95      


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            100                 105                 110         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        115                 120                 125             


Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 
    130                 135                 140                 


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
145                 150                 155                 160 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                165                 170                 175     


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            180                 185                 190         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        195                 200                 205             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    210                 215                 220                 


Pro Gly Lys 
225         


<210>  9
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DP47GS VH

<400>  9

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Gly Ser Gly Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  10
<211>  345
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DP47GS VH

<400>  10
gaggtgcaat tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc       60

tcctgtgcag cctccggatt cacctttagc agttatgcca tgagctgggt ccgccaggct      120

ccagggaagg ggctggagtg ggtctcagct attagtggta gtggtggtag cacatactac      180

gcagactccg tgaagggccg gttcaccatc tccagagaca attccaagaa cacgctgtat      240

ctgcagatga acagcctgag agccgaggac acggccgtat attactgtgc gaaaggcagc      300

ggatttgact actggggcca aggaaccctg gtcaccgtct cgagt                      345


<210>  11
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DP47GS VL

<400>  11

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Ser 
            20                  25                  30          


Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu 
        35                  40                  45              


Ile Tyr Gly Ala Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Ser Ser Pro 
                85                  90                  95      


Leu Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105             


<210>  12
<211>  324
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DP47GS VL

<400>  12
gaaatcgtgt taacgcagtc tccaggcacc ctgtctttgt ctccagggga aagagccacc       60

ctctcttgca gggccagtca gagtgttagc agcagctact tagcctggta ccagcagaaa      120

cctggccagg ctcccaggct cctcatctat ggagcatcca gcagggccac tggcatccca      180

gacaggttca gtggcagtgg atccgggaca gacttcactc tcaccatcag cagactggag      240

cctgaagatt ttgcagtgta ttactgtcag cagtatggta gctcaccgct gacgttcggc      300

caggggacca aagtggaaat caaa                                             324


<210>  13
<211>  592
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DP47GS HC(Fc knob,P329G LALA)-IL2

<400>  13

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Gly Ser Gly Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro 
        115                 120                 125             


Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val 
    130                 135                 140                 


Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala 
145                 150                 155                 160 


Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly 
                165                 170                 175     


Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly 
            180                 185                 190         


Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys 
        195                 200                 205             


Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
    210                 215                 220                 


Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe Leu 
225                 230                 235                 240 


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
                245                 250                 255     


Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys 
            260                 265                 270         


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
        275                 280                 285             


Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
    290                 295                 300                 


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
305                 310                 315                 320 


Val Ser Asn Lys Ala Leu Gly Ala Pro Ile Glu Lys Thr Ile Ser Lys 
                325                 330                 335     


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Cys 
            340                 345                 350         


Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Trp Cys Leu Val Lys 
        355                 360                 365             


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
    370                 375                 380                 


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
385                 390                 395                 400 


Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln 
                405                 410                 415     


Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
            420                 425                 430         


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Gly Gly Gly Gly 
        435                 440                 445             


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Pro Thr Ser Ser 
    450                 455                 460                 


Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His Leu Leu Leu Asp Leu 
465                 470                 475                 480 


Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys Asn Pro Lys Leu Thr 
                485                 490                 495     


Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys Lys Ala Thr Glu Leu 
            500                 505                 510         


Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys Pro Leu Glu Glu Val 
        515                 520                 525             


Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu Arg Pro Arg Asp Leu 
    530                 535                 540                 


Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu Lys Gly Ser Glu Thr 
545                 550                 555                 560 


Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala Thr Ile Val Glu Phe 
                565                 570                 575     


Leu Asn Arg Trp Ile Thr Phe Ala Gln Ser Ile Ile Ser Thr Leu Thr 
            580                 585                 590         


<210>  14
<211>  1776
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DP47GS HC(Fc knob,P329G LALA)-IL2

<400>  14
gaggtgcaat tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc       60

tcctgtgcag cctccggatt cacctttagc agttatgcca tgagctgggt ccgccaggct      120

ccagggaagg ggctggagtg ggtctcagct attagtggta gtggtggtag cacatactac      180

gcagactccg tgaagggccg gttcaccatc tccagagaca attccaagaa cacgctgtat      240

ctgcagatga acagcctgag agccgaggac acggccgtat attactgtgc gaaaggcagc      300

ggatttgact actggggcca aggaaccctg gtcaccgtct cgagtgctag caccaagggc      360

ccatcggtct tccccctggc accctcctcc aagagcacct ctgggggcac agcggccctg      420

ggctgcctgg tcaaggacta cttccccgaa ccggtgacgg tgtcgtggaa ctcaggcgcc      480

ctgaccagcg gcgtgcacac cttcccggct gtcctacagt cctcaggact ctactccctc      540

agcagcgtgg tgaccgtgcc ctccagcagc ttgggcaccc agacctacat ctgcaacgtg      600

aatcacaagc ccagcaacac caaggtggac aagaaagttg agcccaaatc ttgtgacaaa      660

actcacacat gcccaccgtg cccagcacct gaagctgcag ggggaccgtc agtcttcctc      720

ttccccccaa aacccaagga caccctcatg atctcccgga cccctgaggt cacatgcgtg      780

gtggtggacg tgagccacga agaccctgag gtcaagttca actggtacgt ggacggcgtg      840

gaggtgcata atgccaagac aaagccgcgg gaggagcagt acaacagcac gtaccgtgtg      900

gtcagcgtcc tcaccgtcct gcaccaggac tggctgaatg gcaaggagta caagtgcaag      960

gtctccaaca aagccctcgg cgcccccatc gagaaaacca tctccaaagc caaagggcag     1020

ccccgagaac cacaggtgta caccctgccc ccatgccggg atgagctgac caagaaccag     1080

gtcagcctgt ggtgcctggt caaaggcttc tatcccagcg acatcgccgt ggagtgggag     1140

agcaatgggc agccggagaa caactacaag accacgcctc ccgtgctgga ctccgacggc     1200

tccttcttcc tctacagcaa gctcaccgtg gacaagagca ggtggcagca ggggaacgtc     1260

ttctcatgct ccgtgatgca tgaggctctg cacaaccact acacgcagaa gagcctctcc     1320

ctgtctccgg gtggcggcgg aggctccgga ggcggaggtt ctggaggcgg aggctccgca     1380

cctacttcaa gttctacaaa gaaaacacag ctacaactgg agcatttact gctggattta     1440

cagatgattt tgaatggaat taataattac aagaatccca aactcaccag gatgctcaca     1500

tttaagtttt acatgcccaa gaaggccaca gaactgaaac atcttcagtg tctagaagaa     1560

gaactcaaac ctctggagga agtgctaaat ttagctcaaa gcaaaaactt tcacttaaga     1620

cccagggact taatcagcaa tatcaacgta atagttctgg aactaaaggg atctgaaaca     1680

acattcatgt gtgaatatgc tgatgagaca gcaaccattg tagaatttct gaacagatgg     1740

attacctttg cccaaagcat catctcaaca ctgact                               1776


<210>  15
<211>  445
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DP47GS HC(Fc hole, P329G LALA)

<400>  15

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Gly Ser Gly Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro 
        115                 120                 125             


Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val 
    130                 135                 140                 


Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala 
145                 150                 155                 160 


Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly 
                165                 170                 175     


Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly 
            180                 185                 190         


Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys 
        195                 200                 205             


Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
    210                 215                 220                 


Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe Leu 
225                 230                 235                 240 


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
                245                 250                 255     


Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys 
            260                 265                 270         


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
        275                 280                 285             


Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
    290                 295                 300                 


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
305                 310                 315                 320 


Val Ser Asn Lys Ala Leu Gly Ala Pro Ile Glu Lys Thr Ile Ser Lys 
                325                 330                 335     


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Cys Thr Leu Pro Pro Ser 
            340                 345                 350         


Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Ser Cys Ala Val Lys 
        355                 360                 365             


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
    370                 375                 380                 


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
385                 390                 395                 400 


Ser Phe Phe Leu Val Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln 
                405                 410                 415     


Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
            420                 425                 430         


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        435                 440                 445 


<210>  16
<211>  1335
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DP47GS HC(Fc hole, P329G LALA)

<400>  16
gaggtgcaat tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc       60

tcctgtgcag cctccggatt cacctttagc agttatgcca tgagctgggt ccgccaggct      120

ccagggaagg ggctggagtg ggtctcagct attagtggta gtggtggtag cacatactac      180

gcagactccg tgaagggccg gttcaccatc tccagagaca attccaagaa cacgctgtat      240

ctgcagatga acagcctgag agccgaggac acggccgtat attactgtgc gaaaggcagc      300

ggatttgact actggggcca aggaaccctg gtcaccgtct cgagtgctag caccaagggc      360

ccctccgtgt tccccctggc ccccagcagc aagagcacca gcggcggcac agccgctctg      420

ggctgcctgg tcaaggacta cttccccgag cccgtgaccg tgtcctggaa cagcggagcc      480

ctgacctccg gcgtgcacac cttccccgcc gtgctgcaga gttctggcct gtatagcctg      540

agcagcgtgg tcaccgtgcc ttctagcagc ctgggcaccc agacctacat ctgcaacgtg      600

aaccacaagc ccagcaacac caaggtggac aagaaggtgg agcccaagag ctgcgacaaa      660

actcacacat gcccaccgtg cccagcacct gaagctgcag ggggaccgtc agtcttcctc      720

ttccccccaa aacccaagga caccctcatg atctcccgga cccctgaggt cacatgcgtg      780

gtggtggacg tgagccacga agaccctgag gtcaagttca actggtacgt ggacggcgtg      840

gaggtgcata atgccaagac aaagccgcgg gaggagcagt acaacagcac gtaccgtgtg      900

gtcagcgtcc tcaccgtcct gcaccaggac tggctgaatg gcaaggagta caagtgcaag      960

gtctccaaca aagccctcgg cgcccccatc gagaaaacca tctccaaagc caaagggcag     1020

ccccgagaac cacaggtgtg caccctgccc ccatcccggg atgagctgac caagaaccag     1080

gtcagcctct cgtgcgcagt caaaggcttc tatcccagcg acatcgccgt ggagtgggag     1140

agcaatgggc agccggagaa caactacaag accacgcctc ccgtgctgga ctccgacggc     1200

tccttcttcc tcgtgagcaa gctcaccgtg gacaagagca ggtggcagca ggggaacgtc     1260

ttctcatgct ccgtgatgca tgaggctctg cacaaccact acacgcagaa gagcctctcc     1320

ctgtctccgg gtaaa                                                      1335


<210>  17
<211>  592
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DP47GS HC (Fc wt, P329G LALA)-IL2

<400>  17

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Gly Ser Gly Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro 
        115                 120                 125             


Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val 
    130                 135                 140                 


Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala 
145                 150                 155                 160 


Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly 
                165                 170                 175     


Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly 
            180                 185                 190         


Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys 
        195                 200                 205             


Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
    210                 215                 220                 


Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe Leu 
225                 230                 235                 240 


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
                245                 250                 255     


Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys 
            260                 265                 270         


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
        275                 280                 285             


Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
    290                 295                 300                 


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
305                 310                 315                 320 


Val Ser Asn Lys Ala Leu Gly Ala Pro Ile Glu Lys Thr Ile Ser Lys 
                325                 330                 335     


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
            340                 345                 350         


Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
        355                 360                 365             


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
    370                 375                 380                 


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
385                 390                 395                 400 


Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln 
                405                 410                 415     


Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
            420                 425                 430         


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Gly Gly Gly Gly 
        435                 440                 445             


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Pro Thr Ser Ser 
    450                 455                 460                 


Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His Leu Leu Leu Asp Leu 
465                 470                 475                 480 


Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys Asn Pro Lys Leu Thr 
                485                 490                 495     


Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys Lys Ala Thr Glu Leu 
            500                 505                 510         


Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys Pro Leu Glu Glu Val 
        515                 520                 525             


Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu Arg Pro Arg Asp Leu 
    530                 535                 540                 


Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu Lys Gly Ser Glu Thr 
545                 550                 555                 560 


Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala Thr Ile Val Glu Phe 
                565                 570                 575     


Leu Asn Arg Trp Ile Thr Phe Ala Gln Ser Ile Ile Ser Thr Leu Thr 
            580                 585                 590         


<210>  18
<211>  1776
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DP47GS HC (Fc wt, P329G LALA)-IL2

<400>  18
gaggtgcaat tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc       60

tcctgtgcag cctccggatt cacctttagc agttatgcca tgagctgggt ccgccaggct      120

ccagggaagg ggctggagtg ggtctcagct attagtggta gtggtggtag cacatactac      180

gcagactccg tgaagggccg gttcaccatc tccagagaca attccaagaa cacgctgtat      240

ctgcagatga acagcctgag agccgaggac acggccgtat attactgtgc gaaaggcagc      300

ggatttgact actggggcca aggaaccctg gtcaccgtct cgagtgctag caccaagggc      360

ccatcggtct tccccctggc accctcctcc aagagcacct ctgggggcac agcggccctg      420

ggctgcctgg tcaaggacta cttccccgaa ccggtgacgg tgtcgtggaa ctcaggcgcc      480

ctgaccagcg gcgtgcacac cttcccggct gtcctacagt cctcaggact ctactccctc      540

agcagcgtgg tgaccgtgcc ctccagcagc ttgggcaccc agacctacat ctgcaacgtg      600

aatcacaagc ccagcaacac caaggtggac aagaaagttg agcccaaatc ttgtgacaaa      660

actcacacat gcccaccgtg cccagcacct gaagctgcag ggggaccgtc agtcttcctc      720

ttccccccaa aacccaagga caccctcatg atctcccgga cccctgaggt cacatgcgtg      780

gtggtggacg tgagccacga agaccctgag gtcaagttca actggtacgt ggacggcgtg      840

gaggtgcata atgccaagac aaagccgcgg gaggagcagt acaacagcac gtaccgtgtg      900

gtcagcgtcc tcaccgtcct gcaccaggac tggctgaatg gcaaggagta caagtgcaag      960

gtctccaaca aagccctcgg cgcccccatc gagaaaacca tctccaaagc caaagggcag     1020

ccccgagaac cacaggtgta caccctgccc ccatcccggg atgagctgac caagaaccag     1080

gtcagcctga cctgcctggt caaaggcttc tatcccagcg acatcgccgt ggagtgggag     1140

agcaatgggc agccggagaa caactacaag accacgcctc ccgtgctgga ctccgacggc     1200

tccttcttcc tctacagcaa gctcaccgtg gacaagagca ggtggcagca ggggaacgtc     1260

ttctcatgct ccgtgatgca tgaggctctg cacaaccact acacgcagaa gagcctctcc     1320

ctgtctccgg gtggcggcgg aggctccgga ggcggaggtt ctggaggcgg aggctccgca     1380

cctacttcaa gttctacaaa gaaaacacag ctacaactgg agcatttact gctggattta     1440

cagatgattt tgaatggaat taataattac aagaatccca aactcaccag gatgctcaca     1500

tttaagtttt acatgcccaa gaaggccaca gaactgaaac atcttcagtg tctagaagaa     1560

gaactcaaac ctctggagga agtgctaaat ttagctcaaa gcaaaaactt tcacttaaga     1620

cccagggact taatcagcaa tatcaacgta atagttctgg aactaaaggg atctgaaaca     1680

acattcatgt gtgaatatgc tgatgagaca gcaaccattg tagaatttct gaacagatgg     1740

attacctttg cccaaagcat catctcaaca ctgact                               1776


<210>  19
<211>  215
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DP47GS LC

<400>  19

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Ser 
            20                  25                  30          


Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu 
        35                  40                  45              


Ile Tyr Gly Ala Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Ser Ser Pro 
                85                  90                  95      


Leu Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala 
            100                 105                 110         


Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser 
        115                 120                 125             


Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu 
    130                 135                 140                 


Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser 
145                 150                 155                 160 


Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu 
                165                 170                 175     


Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val 
            180                 185                 190         


Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys 
        195                 200                 205             


Ser Phe Asn Arg Gly Glu Cys 
    210                 215 


<210>  20
<211>  645
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DP47GS LC

<400>  20
gaaatcgtgt taacgcagtc tccaggcacc ctgtctttgt ctccagggga aagagccacc       60

ctctcttgca gggccagtca gagtgttagc agcagctact tagcctggta ccagcagaaa      120

cctggccagg ctcccaggct cctcatctat ggagcatcca gcagggccac tggcatccca      180

gacaggttca gtggcagtgg atccgggaca gacttcactc tcaccatcag cagactggag      240

cctgaagatt ttgcagtgta ttactgtcag cagtatggta gctcaccgct gacgttcggc      300

caggggacca aagtggaaat caaacgtacg gtggctgcac catctgtctt catcttcccg      360

ccatctgatg agcagttgaa atctggaact gcctctgttg tgtgcctgct gaataacttc      420

tatcccagag aggccaaagt acagtggaag gtggataacg ccctccaatc gggtaactcc      480

caggagagtg tcacagagca ggacagcaag gacagcacct acagcctcag cagcaccctg      540

acgctgagca aagcagacta cgagaaacac aaagtctacg cctgcgaagt cacccatcag      600

ggcctgagct cgcccgtcac aaagagcttc aacaggggag agtgt                      645


<210>  21
<211>  466
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IL-2R-beta-Fc(hole) fusion protein

<400>  21

Met Asp Met Arg Val Pro Ala Gln Leu Leu Gly Leu Leu Leu Leu Trp 
1               5                   10                  15      


Phe Pro Gly Ala Arg Cys Ala Val Asn Gly Thr Ser Gln Phe Thr Cys 
            20                  25                  30          


Phe Tyr Asn Ser Arg Ala Asn Ile Ser Cys Val Trp Ser Gln Asp Gly 
        35                  40                  45              


Ala Leu Gln Asp Thr Ser Cys Gln Val His Ala Trp Pro Asp Arg Arg 
    50                  55                  60                  


Arg Trp Asn Gln Thr Cys Glu Leu Leu Pro Val Ser Gln Ala Ser Trp 
65                  70                  75                  80  


Ala Cys Asn Leu Ile Leu Gly Ala Pro Asp Ser Gln Lys Leu Thr Thr 
                85                  90                  95      


Val Asp Ile Val Thr Leu Arg Val Leu Cys Arg Glu Gly Val Arg Trp 
            100                 105                 110         


Arg Val Met Ala Ile Gln Asp Phe Lys Pro Phe Glu Asn Leu Arg Leu 
        115                 120                 125             


Met Ala Pro Ile Ser Leu Gln Val Val His Val Glu Thr His Arg Cys 
    130                 135                 140                 


Asn Ile Ser Trp Glu Ile Ser Gln Ala Ser His Tyr Phe Glu Arg His 
145                 150                 155                 160 


Leu Glu Phe Glu Ala Arg Thr Leu Ser Pro Gly His Thr Trp Glu Glu 
                165                 170                 175     


Ala Pro Leu Leu Thr Leu Lys Gln Lys Gln Glu Trp Ile Cys Leu Glu 
            180                 185                 190         


Thr Leu Thr Pro Asp Thr Gln Tyr Glu Phe Gln Val Arg Val Lys Pro 
        195                 200                 205             


Leu Gln Gly Glu Phe Thr Thr Trp Ser Pro Trp Ser Gln Pro Leu Ala 
    210                 215                 220                 


Phe Arg Thr Lys Pro Ala Ala Leu Gly Lys Asp Thr Gly Ala Gln Asp 
225                 230                 235                 240 


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly 
                245                 250                 255     


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
            260                 265                 270         


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
        275                 280                 285             


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
    290                 295                 300                 


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 
305                 310                 315                 320 


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
                325                 330                 335     


Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
            340                 345                 350         


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Cys 
        355                 360                 365             


Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu 
    370                 375                 380                 


Ser Cys Ala Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
385                 390                 395                 400 


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
                405                 410                 415     


Leu Asp Ser Asp Gly Ser Phe Phe Leu Val Ser Lys Leu Thr Val Asp 
            420                 425                 430         


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
        435                 440                 445             


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
    450                 455                 460                 


Gly Lys 
465     


<210>  22
<211>  1401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human IL-2R-beta-Fc(hole) fusion protein

<400>  22
atggacatga gggtccccgc tcagctcctg ggcctcctgc tgctctggtt cccaggtgcc       60

aggtgtgcgg tgaatggcac ttcccagttc acatgcttct acaactcgag agccaacatc      120

tcctgtgtct ggagccaaga tggggctctg caggacactt cctgccaagt ccatgcctgg      180

ccggacagac ggcggtggaa ccaaacctgt gagctgctcc ccgtgagtca agcatcctgg      240

gcctgcaacc tgatcctcgg agccccagat tctcagaaac tgaccacagt tgacatcgtc      300

accctgaggg tgctgtgccg tgagggggtg cgatggaggg tgatggccat ccaggacttc      360

aagccctttg agaaccttcg cctgatggcc cccatctccc tccaagttgt ccacgtggag      420

acccacagat gcaacataag ctgggaaatc tcccaagcct cccactactt tgaaagacac      480

ctggagttcg aggcccggac gctgtcccca ggccacacct gggaggaggc ccccctgctg      540

actctcaagc agaagcagga atggatctgc ctggagacgc tcaccccaga cacccagtat      600

gagtttcagg tgcgggtcaa gcctctgcaa ggcgagttca cgacctggag cccctggagc      660

cagcccctgg ccttcagaac aaagcctgca gcccttggga aggacaccgg agctcaggac      720

aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc gtcagtcttc      780

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc      840

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc      900

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt      960

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc     1020

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg     1080

cagccccgag aaccacaggt gtgcaccctg cccccatccc gggatgagct gaccaagaac     1140

caggtcagcc tctcgtgcgc agtcaaaggc ttctatccca gcgacatcgc cgtggagtgg     1200

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac     1260

ggctccttct tcctcgtgag caagctcacc gtggacaaga gcaggtggca gcaggggaac     1320

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc     1380

tccctgtctc cgggtaaatg a                                               1401


<210>  23
<211>  492
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IL-2R-gamma-Fc(knob) fusion protein

<400>  23

Met Leu Lys Pro Ser Leu Pro Phe Thr Ser Leu Leu Phe Leu Gln Leu 
1               5                   10                  15      


Pro Leu Leu Gly Val Gly Leu Asn Thr Thr Ile Leu Thr Pro Asn Gly 
            20                  25                  30          


Asn Glu Asp Thr Thr Ala Asp Phe Phe Leu Thr Thr Met Pro Thr Asp 
        35                  40                  45              


Ser Leu Ser Val Ser Thr Leu Pro Leu Pro Glu Val Gln Cys Phe Val 
    50                  55                  60                  


Phe Asn Val Glu Tyr Met Asn Cys Thr Trp Asn Ser Ser Ser Glu Pro 
65                  70                  75                  80  


Gln Pro Thr Asn Leu Thr Leu His Tyr Trp Tyr Lys Asn Ser Asp Asn 
                85                  90                  95      


Asp Lys Val Gln Lys Cys Ser His Tyr Leu Phe Ser Glu Glu Ile Thr 
            100                 105                 110         


Ser Gly Cys Gln Leu Gln Lys Lys Glu Ile His Leu Tyr Gln Thr Phe 
        115                 120                 125             


Val Val Gln Leu Gln Asp Pro Arg Glu Pro Arg Arg Gln Ala Thr Gln 
    130                 135                 140                 


Met Leu Lys Leu Gln Asn Leu Val Ile Pro Trp Ala Pro Glu Asn Leu 
145                 150                 155                 160 


Thr Leu His Lys Leu Ser Glu Ser Gln Leu Glu Leu Asn Trp Asn Asn 
                165                 170                 175     


Arg Phe Leu Asn His Cys Leu Glu His Leu Val Gln Tyr Arg Thr Asp 
            180                 185                 190         


Trp Asp His Ser Trp Thr Glu Gln Ser Val Asp Tyr Arg His Lys Phe 
        195                 200                 205             


Ser Leu Pro Ser Val Asp Gly Gln Lys Arg Tyr Thr Phe Arg Val Arg 
    210                 215                 220                 


Ser Arg Phe Asn Pro Leu Cys Gly Ser Ala Gln His Trp Ser Glu Trp 
225                 230                 235                 240 


Ser His Pro Ile His Trp Gly Ser Asn Thr Ser Lys Glu Asn Pro Phe 
                245                 250                 255     


Leu Phe Ala Leu Glu Ala Gly Ala Gln Asp Lys Thr His Thr Cys Pro 
            260                 265                 270         


Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe 
        275                 280                 285             


Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val 
    290                 295                 300                 


Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe 
305                 310                 315                 320 


Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro 
                325                 330                 335     


Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr 
            340                 345                 350         


Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val 
        355                 360                 365             


Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala 
    370                 375                 380                 


Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Cys Arg 
385                 390                 395                 400 


Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Trp Cys Leu Val Lys Gly 
                405                 410                 415     


Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro 
            420                 425                 430         


Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser 
        435                 440                 445             


Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln 
    450                 455                 460                 


Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His 
465                 470                 475                 480 


Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                485                 490         


<210>  24
<211>  1479
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human IL-2R-gamma-Fc(knob) fusion protein

<400>  24
atgttgaagc catcattacc attcacatcc ctcttattcc tgcagctgcc cctgctggga       60

gtggggctga acacgacaat tctgacgccc aatgggaatg aagacaccac agctgatttc      120

ttcctgacca ctatgcccac tgactccctc agtgtttcca ctctgcccct cccagaggtt      180

cagtgttttg tgttcaatgt cgagtacatg aattgcactt ggaacagcag ctctgagccc      240

cagcctacca acctcactct gcattattgg tacaagaact cggataatga taaagtccag      300

aagtgcagcc actatctatt ctctgaagaa atcacttctg gctgtcagtt gcaaaaaaag      360

gagatccacc tctaccaaac atttgttgtt cagctccagg acccacggga acccaggaga      420

caggccacac agatgctaaa actgcagaat ctggtgatcc cctgggctcc agagaaccta      480

acacttcaca aactgagtga atcccagcta gaactgaact ggaacaacag attcttgaac      540

cactgtttgg agcacttggt gcagtaccgg actgactggg accacagctg gactgaacaa      600

tcagtggatt atagacataa gttctccttg cctagtgtgg atgggcagaa acgctacacg      660

tttcgtgttc ggagccgctt taacccactc tgtggaagtg ctcagcattg gagtgaatgg      720

agccacccaa tccactgggg gagcaatact tcaaaagaga atcctttcct gtttgcattg      780

gaagccggag ctcaggacaa aactcacaca tgcccaccgt gcccagcacc tgaactcctg      840

gggggaccgt cagtcttcct cttcccccca aaacccaagg acaccctcat gatctcccgg      900

acccctgagg tcacatgcgt ggtggtggac gtgagccacg aagaccctga ggtcaagttc      960

aactggtacg tggacggcgt ggaggtgcat aatgccaaga caaagccgcg ggaggagcag     1020

tacaacagca cgtaccgtgt ggtcagcgtc ctcaccgtcc tgcaccagga ctggctgaat     1080

ggcaaggagt acaagtgcaa ggtctccaac aaagccctcc cagcccccat cgagaaaacc     1140

atctccaaag ccaaagggca gccccgagaa ccacaggtgt acaccctgcc cccatgccgg     1200

gatgagctga ccaagaacca ggtcagcctg tggtgcctgg tcaaaggctt ctatcccagc     1260

gacatcgccg tggagtggga gagcaatggg cagccggaga acaactacaa gaccacgcct     1320

cccgtgctgg actccgacgg ctccttcttc ctctacagca agctcaccgt ggacaagagc     1380

aggtggcagc aggggaacgt cttctcatgc tccgtgatgc atgaggctct gcacaaccac     1440

tacacgcaga agagcctctc cctgtctccg ggtaaatga                            1479


<210>  25
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IL-2R alpha subunit + Avi-tag + His-tag

<400>  25

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Glu Leu Cys Asp Asp Asp Pro Pro Glu Ile Pro His Ala 
            20                  25                  30          


Thr Phe Lys Ala Met Ala Tyr Lys Glu Gly Thr Met Leu Asn Cys Glu 
        35                  40                  45              


Cys Lys Arg Gly Phe Arg Arg Ile Lys Ser Gly Ser Leu Tyr Met Leu 
    50                  55                  60                  


Cys Thr Gly Asn Ser Ser His Ser Ser Trp Asp Asn Gln Cys Gln Cys 
65                  70                  75                  80  


Thr Ser Ser Ala Thr Arg Asn Thr Thr Lys Gln Val Thr Pro Gln Pro 
                85                  90                  95      


Glu Glu Gln Lys Glu Arg Lys Thr Thr Glu Met Gln Ser Pro Met Gln 
            100                 105                 110         


Pro Val Asp Gln Ala Ser Leu Pro Gly His Cys Arg Glu Pro Pro Pro 
        115                 120                 125             


Trp Glu Asn Glu Ala Thr Glu Arg Ile Tyr His Phe Val Val Gly Gln 
    130                 135                 140                 


Met Val Tyr Tyr Gln Cys Val Gln Gly Tyr Arg Ala Leu His Arg Gly 
145                 150                 155                 160 


Pro Ala Glu Ser Val Cys Lys Met Thr His Gly Lys Thr Arg Trp Thr 
                165                 170                 175     


Gln Pro Gln Leu Ile Cys Thr Gly Val Asp Glu Gln Leu Tyr Phe Gln 
            180                 185                 190         


Gly Gly Ser Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp 
        195                 200                 205             


His Glu Ala Arg Ala His His His His His His 
    210                 215                 


<210>  26
<211>  660
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human IL-2R alpha subunit + Avi-tag + His-tag

<400>  26
atgggatgga gctgtatcat cctcttcttg gtagcaacag ctaccggtgt gcattccgag       60

ctctgtgacg atgacccgcc agagatccca cacgccacat tcaaagccat ggcctacaag      120

gaaggaacca tgttgaactg tgaatgcaag agaggtttcc gcagaataaa aagcgggtca      180

ctctatatgc tctgtacagg aaactctagc cactcgtcct gggacaacca atgtcaatgc      240

acaagctctg ccactcggaa cacaacgaaa caagtgacac ctcaacctga agaacagaaa      300

gaaaggaaaa ccacagaaat gcaaagtcca atgcagccag tggaccaagc gagccttcca      360

ggtcactgca gggaacctcc accatgggaa aatgaagcca cagagagaat ttatcatttc      420

gtggtggggc agatggttta ttatcagtgc gtccagggat acagggctct acacagaggt      480

cctgctgaga gcgtctgcaa aatgacccac gggaagacaa ggtggaccca gccccagctc      540

atatgcacag gtgtcgacga acagttatat tttcagggcg gctcaggcct gaacgacatc      600

ttcgaggccc agaagatcga gtggcacgag gctcgagctc accaccatca ccatcactga      660


<210>  27
<211>  473
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Murine IL-2R-beta-Fc(hole) fusion protein

<400>  27

Met Asp Met Arg Val Pro Ala Gln Leu Leu Gly Leu Leu Leu Leu Trp 
1               5                   10                  15      


Phe Pro Leu Leu Leu Leu Trp Phe Pro Gly Ala Arg Cys Ala Val Lys 
            20                  25                  30          


Asn Cys Ser His Leu Glu Cys Phe Tyr Asn Ser Arg Ala Asn Val Ser 
        35                  40                  45              


Cys Met Trp Ser His Glu Glu Ala Leu Asn Val Thr Thr Cys His Val 
    50                  55                  60                  


His Ala Lys Ser Asn Leu Arg His Trp Asn Lys Thr Cys Glu Leu Thr 
65                  70                  75                  80  


Leu Val Arg Gln Ala Ser Trp Ala Cys Asn Leu Ile Leu Gly Ser Phe 
                85                  90                  95      


Pro Glu Ser Gln Ser Leu Thr Ser Val Asp Leu Leu Asp Ile Asn Val 
            100                 105                 110         


Val Cys Trp Glu Glu Lys Gly Trp Arg Arg Val Lys Thr Cys Asp Phe 
        115                 120                 125             


His Pro Phe Asp Asn Leu Arg Leu Val Ala Pro His Ser Leu Gln Val 
    130                 135                 140                 


Leu His Ile Asp Thr Gln Arg Cys Asn Ile Ser Trp Lys Val Ser Gln 
145                 150                 155                 160 


Val Ser His Tyr Ile Glu Pro Tyr Leu Glu Phe Glu Ala Arg Arg Arg 
                165                 170                 175     


Leu Leu Gly His Ser Trp Glu Asp Ala Ser Val Leu Ser Leu Lys Gln 
            180                 185                 190         


Arg Gln Gln Trp Leu Phe Leu Glu Met Leu Ile Pro Ser Thr Ser Tyr 
        195                 200                 205             


Glu Val Gln Val Arg Val Lys Ala Gln Arg Asn Asn Thr Gly Thr Trp 
    210                 215                 220                 


Ser Pro Trp Ser Gln Pro Leu Thr Phe Arg Thr Arg Pro Ala Asp Pro 
225                 230                 235                 240 


Met Lys Glu Gly Ala Gln Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
                245                 250                 255     


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
            260                 265                 270         


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
        275                 280                 285             


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
    290                 295                 300                 


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
305                 310                 315                 320 


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
                325                 330                 335     


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
            340                 345                 350         


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
        355                 360                 365             


Pro Arg Glu Pro Gln Val Cys Thr Leu Pro Pro Ser Arg Asp Glu Leu 
    370                 375                 380                 


Thr Lys Asn Gln Val Ser Leu Ser Cys Ala Val Lys Gly Phe Tyr Pro 
385                 390                 395                 400 


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
                405                 410                 415     


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
            420                 425                 430         


Val Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
        435                 440                 445             


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
    450                 455                 460                 


Lys Ser Leu Ser Leu Ser Pro Gly Lys 
465                 470             


<210>  28
<211>  1422
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Murine IL-2R-beta-Fc(hole) fusion protein

<400>  28
atggacatga gggtccccgc tcagctcctg ggcctcctgc tgctctggtt ccccctcctg       60

ctgctctggt tcccaggtgc caggtgtgca gtgaaaaact gttcccatct tgaatgcttc      120

tacaactcaa gagccaatgt ctcttgcatg tggagccatg aagaggctct gaatgtcaca      180

acctgccacg tccatgccaa gtcgaacctg cgacactgga acaaaacctg tgagctaact      240

cttgtgaggc aggcatcctg ggcctgcaac ctgatcctcg ggtcgttccc agagtcccag      300

tcactgacct ccgtggacct ccttgacata aatgtggtgt gctgggaaga gaagggttgg      360

cgtagggtaa agacctgcga cttccatccc tttgacaacc ttcgcctggt ggcccctcat      420

tccctccaag ttctgcacat tgatacccag agatgtaaca taagctggaa ggtctcccag      480

gtctctcact acattgaacc atacttggaa tttgaggccc gtagacgtct tctgggccac      540

agctgggagg atgcatccgt attaagcctc aagcagagac agcagtggct cttcttggag      600

atgctgatcc ctagtacctc atatgaggtc caggtgaggg tcaaagctca acgaaacaat      660

accgggacct ggagtccctg gagccagccc ctgacctttc ggacaaggcc agcagatccc      720

atgaaggagg gagctcagga caaaactcac acatgcccac cgtgcccagc acctgaactc      780

ctggggggac cgtcagtctt cctcttcccc ccaaaaccca aggacaccct catgatctcc      840

cggacccctg aggtcacatg cgtggtggtg gacgtgagcc acgaagaccc tgaggtcaag      900

ttcaactggt acgtggacgg cgtggaggtg cataatgcca agacaaagcc gcgggaggag      960

cagtacaaca gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca ggactggctg     1020

aatggcaagg agtacaagtg caaggtctcc aacaaagccc tcccagcccc catcgagaaa     1080

accatctcca aagccaaagg gcagccccga gaaccacagg tgtgcaccct gcccccatcc     1140

cgggatgagc tgaccaagaa ccaggtcagc ctctcgtgcg cagtcaaagg cttctatccc     1200

agcgacatcg ccgtggagtg ggagagcaat gggcagccgg agaacaacta caagaccacg     1260

cctcccgtgc tggactccga cggctccttc ttcctcgtga gcaagctcac cgtggacaag     1320

agcaggtggc agcaggggaa cgtcttctca tgctccgtga tgcatgaggc tctgcacaac     1380

cactacacgc agaagagcct ctccctgtct ccgggtaaat ga                        1422


<210>  29
<211>  500
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Murine IL-2R-gamma-Fc(knob) fusion protein

<400>  29

Met Asp Met Arg Val Pro Ala Gln Leu Leu Gly Leu Leu Leu Leu Trp 
1               5                   10                  15      


Phe Pro Leu Leu Leu Leu Trp Phe Pro Gly Ala Arg Cys Trp Ser Ser 
            20                  25                  30          


Lys Val Leu Met Ser Ser Ala Asn Glu Asp Ile Lys Ala Asp Leu Ile 
        35                  40                  45              


Leu Thr Ser Thr Ala Pro Glu His Leu Ser Ala Pro Thr Leu Pro Leu 
    50                  55                  60                  


Pro Glu Val Gln Cys Phe Val Phe Asn Ile Glu Tyr Met Asn Cys Thr 
65                  70                  75                  80  


Trp Asn Ser Ser Ser Glu Pro Gln Ala Thr Asn Leu Thr Leu His Tyr 
                85                  90                  95      


Arg Tyr Lys Val Ser Asp Asn Asn Thr Phe Gln Glu Cys Ser His Tyr 
            100                 105                 110         


Leu Phe Ser Lys Glu Ile Thr Ser Gly Cys Gln Ile Gln Lys Glu Asp 
        115                 120                 125             


Ile Gln Leu Tyr Gln Thr Phe Val Val Gln Leu Gln Asp Pro Gln Lys 
    130                 135                 140                 


Pro Gln Arg Arg Ala Val Gln Lys Leu Asn Leu Gln Asn Leu Val Ile 
145                 150                 155                 160 


Pro Arg Ala Pro Glu Asn Leu Thr Leu Ser Asn Leu Ser Glu Ser Gln 
                165                 170                 175     


Leu Glu Leu Arg Trp Lys Ser Arg His Ile Lys Glu Arg Cys Leu Gln 
            180                 185                 190         


Tyr Leu Val Gln Tyr Arg Ser Asn Arg Asp Arg Ser Trp Thr Glu Leu 
        195                 200                 205             


Ile Val Asn His Glu Pro Arg Phe Ser Leu Pro Ser Val Asp Glu Leu 
    210                 215                 220                 


Lys Arg Tyr Thr Phe Arg Val Arg Ser Arg Tyr Asn Pro Ile Cys Gly 
225                 230                 235                 240 


Ser Ser Gln Gln Trp Ser Lys Trp Ser Gln Pro Val His Trp Gly Ser 
                245                 250                 255     


His Thr Val Glu Glu Asn Pro Ser Leu Phe Ala Leu Glu Ala Gly Ala 
            260                 265                 270         


Gln Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu 
        275                 280                 285             


Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
    290                 295                 300                 


Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
305                 310                 315                 320 


His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu 
                325                 330                 335     


Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr 
            340                 345                 350         


Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 
        355                 360                 365             


Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro 
    370                 375                 380                 


Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln 
385                 390                 395                 400 


Val Tyr Thr Leu Pro Pro Cys Arg Asp Glu Leu Thr Lys Asn Gln Val 
                405                 410                 415     


Ser Leu Trp Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
            420                 425                 430         


Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
        435                 440                 445             


Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 
    450                 455                 460                 


Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
465                 470                 475                 480 


Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
                485                 490                 495     


Ser Pro Gly Lys 
            500 


<210>  30
<211>  1503
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Murine IL-2R-gamma-Fc(knob) fusion protein

<400>  30
atggacatga gggtccccgc tcagctcctg ggcctcctgc tgctctggtt ccccctcctg       60

ctgctctggt tcccaggtgc caggtgttgg agttccaagg tcctcatgtc cagtgcgaat      120

gaagacatca aagctgattt gatcctgact tctacagccc ctgaacacct cagtgctcct      180

actctgcccc ttccagaggt tcagtgcttt gtgttcaaca tagagtacat gaattgcact      240

tggaatagca gttctgagcc tcaggcaacc aacctcacgc tgcactatag gtacaaggta      300

tctgataata atacattcca ggagtgcagt cactatttgt tctccaaaga gattacttct      360

ggctgtcaga tacaaaaaga agatatccag ctctaccaga catttgttgt ccagctccag      420

gacccccaga aaccccagag gcgagctgta cagaagctaa acctacagaa tcttgtgatc      480

ccacgggctc cagaaaatct aacactcagc aatctgagtg aatcccagct agagctgaga      540

tggaaaagca gacatattaa agaacgctgt ttacaatact tggtgcagta ccggagcaac      600

agagatcgaa gctggacgga actaatagtg aatcatgaac ctagattctc cctgcctagt      660

gtggatgagc tgaaacggta cacatttcgg gttcggagcc gctataaccc aatctgtgga      720

agttctcaac agtggagtaa atggagccag cctgtccact gggggagtca tactgtagag      780

gagaatcctt ccttgtttgc actggaagct ggagctcagg acaaaactca cacatgccca      840

ccgtgcccag cacctgaact cctgggggga ccgtcagtct tcctcttccc cccaaaaccc      900

aaggacaccc tcatgatctc ccggacccct gaggtcacat gcgtggtggt ggacgtgagc      960

cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt gcataatgcc     1020

aagacaaagc cgcgggagga gcagtacaac agcacgtacc gtgtggtcag cgtcctcacc     1080

gtcctgcacc aggactggct gaatggcaag gagtacaagt gcaaggtctc caacaaagcc     1140

ctcccagccc ccatcgagaa aaccatctcc aaagccaaag ggcagccccg agaaccacag     1200

gtgtacaccc tgcccccatg ccgggatgag ctgaccaaga accaggtcag cctgtggtgc     1260

ctggtcaaag gcttctatcc cagcgacatc gccgtggagt gggagagcaa tgggcagccg     1320

gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt cttcctctac     1380

agcaagctca ccgtggacaa gagcaggtgg cagcagggga acgtcttctc atgctccgtg     1440

atgcatgagg ctctgcacaa ccactacacg cagaagagcc tctccctgtc tccgggtaaa     1500

tga                                                                   1503


<210>  31
<211>  213
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Murine IL-2R alpha subunit + Avi-tag + His-tag

<400>  31

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Glu Leu Cys Leu Tyr Asp Pro Pro Glu Val Pro Asn Ala 
            20                  25                  30          


Thr Phe Lys Ala Leu Ser Tyr Lys Asn Gly Thr Ile Leu Asn Cys Glu 
        35                  40                  45              


Cys Lys Arg Gly Phe Arg Arg Leu Lys Glu Leu Val Tyr Met Arg Cys 
    50                  55                  60                  


Leu Gly Asn Ser Trp Ser Ser Asn Cys Gln Cys Thr Ser Asn Ser His 
65                  70                  75                  80  


Asp Lys Ser Arg Lys Gln Val Thr Ala Gln Leu Glu His Gln Lys Glu 
                85                  90                  95      


Gln Gln Thr Thr Thr Asp Met Gln Lys Pro Thr Gln Ser Met His Gln 
            100                 105                 110         


Glu Asn Leu Thr Gly His Cys Arg Glu Pro Pro Pro Trp Lys His Glu 
        115                 120                 125             


Asp Ser Lys Arg Ile Tyr His Phe Val Glu Gly Gln Ser Val His Tyr 
    130                 135                 140                 


Glu Cys Ile Pro Gly Tyr Lys Ala Leu Gln Arg Gly Pro Ala Ile Ser 
145                 150                 155                 160 


Ile Cys Lys Met Lys Cys Gly Lys Thr Gly Trp Thr Gln Pro Gln Leu 
                165                 170                 175     


Thr Cys Val Asp Glu Gln Leu Tyr Phe Gln Gly Gly Ser Gly Leu Asn 
            180                 185                 190         


Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu Ala Arg Ala His 
        195                 200                 205             


His His His His His 
    210             


<210>  32
<211>  642
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Murine IL-2R alpha subunit + Avi-tag + His-tag

<400>  32
atgggatgga gctgtatcat cctcttcttg gtagcaacag ctaccggtgt gcattccgaa       60

ctgtgtctgt atgacccacc cgaggtcccc aatgccacat tcaaagccct ctcctacaag      120

aacggcacca tcctaaactg tgaatgcaag agaggtttcc gaagactaaa ggaattggtc      180

tatatgcgtt gcttaggaaa ctcctggagc agcaactgcc agtgcaccag caactcccat      240

gacaaatcga gaaagcaagt tacagctcaa cttgaacacc agaaagagca acaaaccaca      300

acagacatgc agaagccaac acagtctatg caccaagaga accttacagg tcactgcagg      360

gagccacctc cttggaaaca tgaagattcc aagagaatct atcatttcgt ggaaggacag      420

agtgttcact acgagtgtat tccgggatac aaggctctac agagaggtcc tgctattagc      480

atctgcaaga tgaagtgtgg gaaaacgggg tggactcagc cccagctcac atgtgtcgac      540

gaacagttat attttcaggg cggctcaggc ctgaacgaca tcttcgaggc ccagaagatc      600

gagtggcacg aggctcgagc tcaccaccat caccatcact ga                         642


<210>  33
<211>  480
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cynomolgous IL-2R-beta-Fc(knob) fusion protein + Avi-tag

<400>  33

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Ala Val Asn Gly Thr Ser Arg Phe Thr Cys Phe Tyr Asn 
            20                  25                  30          


Ser Arg Ala Asn Ile Ser Cys Val Trp Ser Gln Asp Gly Ala Leu Gln 
        35                  40                  45              


Asp Thr Ser Cys Gln Val His Ala Trp Pro Asp Arg Arg Arg Trp Asn 
    50                  55                  60                  


Gln Thr Cys Glu Leu Leu Pro Val Ser Gln Ala Ser Trp Ala Cys Asn 
65                  70                  75                  80  


Leu Ile Leu Gly Thr Pro Asp Ser Gln Lys Leu Thr Ala Val Asp Ile 
                85                  90                  95      


Val Thr Leu Arg Val Met Cys Arg Glu Gly Val Arg Trp Arg Met Met 
            100                 105                 110         


Ala Ile Gln Asp Phe Lys Pro Phe Glu Asn Leu Arg Leu Met Ala Pro 
        115                 120                 125             


Ile Ser Leu Gln Val Val His Val Glu Thr His Arg Cys Asn Ile Ser 
    130                 135                 140                 


Trp Lys Ile Ser Gln Ala Ser His Tyr Phe Glu Arg His Leu Glu Phe 
145                 150                 155                 160 


Glu Ala Arg Thr Leu Ser Pro Gly His Thr Trp Glu Glu Ala Pro Leu 
                165                 170                 175     


Met Thr Leu Lys Gln Lys Gln Glu Trp Ile Cys Leu Glu Thr Leu Thr 
            180                 185                 190         


Pro Asp Thr Gln Tyr Glu Phe Gln Val Arg Val Lys Pro Leu Gln Gly 
        195                 200                 205             


Glu Phe Thr Thr Trp Ser Pro Trp Ser Gln Pro Leu Ala Phe Arg Thr 
    210                 215                 220                 


Lys Pro Ala Ala Leu Gly Lys Asp Thr Gly Ala Gln Asp Lys Thr His 
225                 230                 235                 240 


Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val 
                245                 250                 255     


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
            260                 265                 270         


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
        275                 280                 285             


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
    290                 295                 300                 


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
305                 310                 315                 320 


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
                325                 330                 335     


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
            340                 345                 350         


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
        355                 360                 365             


Pro Cys Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Trp Cys Leu 
    370                 375                 380                 


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
385                 390                 395                 400 


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
                405                 410                 415     


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
            420                 425                 430         


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
        435                 440                 445             


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Ser 
    450                 455                 460                 


Gly Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu 
465                 470                 475                 480 


<210>  34
<211>  1443
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cynomolgous IL-2R-beta-Fc(knob) fusion protein + Avi-tag

<400>  34
atgggatgga gctgtatcat cctcttcttg gtagcaacag ctaccggtgt gcattccgcg       60

gtcaacggca cttcccggtt cacatgcttc tacaactcga gagccaacat ctcctgtgtc      120

tggagccaag atggggctct gcaggacact tcctgccaag tccacgcctg gccggacaga      180

cggcggtgga accaaacctg tgagctgctc cctgtgagtc aagcatcctg ggcctgcaac      240

ctgatcctcg gaaccccaga ttctcagaaa ctgaccgcag tggatatcgt caccctgagg      300

gtgatgtgcc gtgaaggggt gcgatggagg atgatggcca tccaggactt caaacccttt      360

gagaaccttc gcctgatggc ccccatctcc ctccaagtcg tccacgtgga gacccacaga      420

tgcaacataa gctggaaaat ctcccaagcc tcccactact ttgaaagaca cctggagttt      480

gaggcccgga cgctgtcccc aggccacacc tgggaggagg cccccctgat gaccctcaag      540

cagaagcagg aatggatctg cctggagacg ctcaccccag acacccagta tgagtttcag      600

gtgcgggtca agcctctgca aggcgagttc acgacctgga gcccctggag ccagcccctg      660

gccttcagga caaagcctgc agcccttggg aaggacaccg gagctcagga caaaactcac      720

acatgcccac cgtgcccagc acctgaactc ctggggggac cgtcagtctt cctcttcccc      780

ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg      840

gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg      900

cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc      960

gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg caaggtctcc     1020

aacaaagccc tcccagcccc catcgagaaa accatctcca aagccaaagg gcagccccga     1080

gaaccacagg tgtacaccct gcccccatgc cgggatgagc tgaccaagaa ccaggtcagc     1140

ctgtggtgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg ggagagcaat     1200

gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc     1260

ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa cgtcttctca     1320

tgctccgtga tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct     1380

ccgggtaaat ccggaggcct gaacgacatc ttcgaggccc agaagattga atggcacgag     1440

tga                                                                   1443


<210>  35
<211>  489
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cynomolgous IL-2R-gamma-Fc(hole) fusion protein

<400>  35

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Leu Asn Thr Thr Ile Leu Thr Pro Asn Gly Asn Glu Asp 
            20                  25                  30          


Ala Thr Thr Asp Phe Phe Leu Thr Ser Met Pro Thr Asp Ser Leu Ser 
        35                  40                  45              


Val Ser Thr Leu Pro Leu Pro Glu Val Gln Cys Phe Val Phe Asn Val 
    50                  55                  60                  


Glu Tyr Met Asn Cys Thr Trp Asn Ser Ser Ser Glu Pro Gln Pro Thr 
65                  70                  75                  80  


Asn Leu Thr Leu His Tyr Trp Tyr Lys Asn Ser Asp Asn Asp Lys Val 
                85                  90                  95      


Gln Lys Cys Ser His Tyr Leu Phe Ser Glu Glu Ile Thr Ser Gly Cys 
            100                 105                 110         


Gln Leu Gln Lys Lys Glu Ile His Leu Tyr Gln Thr Phe Val Val Gln 
        115                 120                 125             


Leu Gln Asp Pro Arg Glu Pro Arg Arg Gln Ala Thr Gln Met Leu Lys 
    130                 135                 140                 


Leu Gln Asn Leu Val Ile Pro Trp Ala Pro Glu Asn Leu Thr Leu Arg 
145                 150                 155                 160 


Lys Leu Ser Glu Ser Gln Leu Glu Leu Asn Trp Asn Asn Arg Phe Leu 
                165                 170                 175     


Asn His Cys Leu Glu His Leu Val Gln Tyr Arg Thr Asp Trp Asp His 
            180                 185                 190         


Ser Trp Thr Glu Gln Ser Val Asp Tyr Arg His Lys Phe Ser Leu Pro 
        195                 200                 205             


Ser Val Asp Gly Gln Lys Arg Tyr Thr Phe Arg Val Arg Ser Arg Phe 
    210                 215                 220                 


Asn Pro Leu Cys Gly Ser Ala Gln His Trp Ser Glu Trp Ser His Pro 
225                 230                 235                 240 


Ile His Trp Gly Ser Asn Ser Ser Lys Glu Asn Pro Phe Leu Phe Ala 
                245                 250                 255     


Leu Glu Ala Gly Ala Gln Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
            260                 265                 270         


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
        275                 280                 285             


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
    290                 295                 300                 


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
305                 310                 315                 320 


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
                325                 330                 335     


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
            340                 345                 350         


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
        355                 360                 365             


Ala Leu Gly Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
    370                 375                 380                 


Pro Arg Glu Pro Gln Val Cys Thr Leu Pro Pro Ser Arg Asp Glu Leu 
385                 390                 395                 400 


Thr Lys Asn Gln Val Ser Leu Ser Cys Ala Val Lys Gly Phe Tyr Pro 
                405                 410                 415     


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
            420                 425                 430         


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
        435                 440                 445             


Val Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
    450                 455                 460                 


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
465                 470                 475                 480 


Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                485                 


<210>  36
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cynomolgous IL-2R-gamma-Fc(hole) fusion protein

<400>  36
atgggatgga gctgtatcat cctcttcttg gtagcaacag ctaccggtgt gcattccctg       60

aacacgacaa ttctgacgcc caatgggaat gaagacgcca caactgattt cttcctgacc      120

tctatgccca ctgactccct cagtgtttcc actctgcccc tcccagaggt tcagtgtttt      180

gtgttcaatg tcgagtacat gaattgcact tggaacagca gctctgagcc ccagcctacc      240

aacctcactc tgcattattg gtacaagaat tcggataatg ataaagtcca gaagtgcagc      300

cactatctat tctctgaaga aatcacttct ggctgtcagt tgcaaaaaaa ggagatccac      360

ctctaccaaa cgtttgttgt tcagctccag gacccacggg aacccaggag acaggccaca      420

cagatgctaa aactgcagaa tctggtgatc ccctgggctc cggagaacct aacacttcgc      480

aaactgagtg aatcccagct agaactgaac tggaacaaca gattcttgaa ccactgtttg      540

gagcacttgg tgcagtaccg gactgactgg gaccacagct ggactgaaca atcagtggat      600

tatagacata agttctcctt gcctagtgtg gatgggcaga aacgctacac gtttcgtgtc      660

cggagccgct ttaacccact ctgtggaagt gctcagcatt ggagtgaatg gagccaccca      720

atccactggg ggagcaatag ttcaaaagag aatcctttcc tgtttgcatt ggaagccgga      780

gctcaggaca aaactcacac atgcccaccg tgcccagcac ctgaactcct ggggggaccg      840

tcagtcttcc tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag      900

gtcacatgcg tggtggtgga cgtgagccac gaagaccctg aggtcaagtt caactggtac      960

gtggacggcg tggaggtgca taatgccaag acaaagccgc gggaggagca gtacaacagc     1020

acgtaccgtg tggtcagcgt cctcaccgtc ctgcaccagg actggctgaa tggcaaggag     1080

tacaagtgca aggtctccaa caaagccctc ggcgccccca tcgagaaaac catctccaaa     1140

gccaaagggc agccccgaga accacaggtg tgcaccctgc ccccatcccg ggatgagctg     1200

accaagaacc aggtcagcct ctcgtgcgca gtcaaaggct tctatcccag cgacatcgcc     1260

gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacgcc tcccgtgctg     1320

gactccgacg gctccttctt cctcgtgagc aagctcaccg tggacaagag caggtggcag     1380

caggggaacg tcttctcatg ctccgtgatg catgaggctc tgcacaacca ctacacgcag     1440

aagagcctct ccctgtctcc gggtaaatga                                      1470


<210>  37
<211>  217
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cynomolgous IL-2R alpha subunit + Avi-tag + His-tag

<400>  37

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Glu Leu Cys Asp Asp Asp Pro Pro Lys Ile Thr His Ala Thr Phe Lys 
            20                  25                  30          


Ala Met Ala Tyr Lys Glu Gly Thr Met Leu Asn Cys Glu Cys Lys Arg 
        35                  40                  45              


Gly Phe Arg Arg Ile Lys Ser Gly Ser Pro Tyr Met Leu Cys Thr Gly 
    50                  55                  60                  


Asn Ser Ser His Ser Ser Trp Asp Asn Gln Cys Gln Cys Thr Ser Ser 
65                  70                  75                  80  


Ala Ala Arg Asn Thr Thr Lys Gln Val Thr Pro Gln Pro Glu Glu Gln 
                85                  90                  95      


Lys Glu Arg Lys Thr Thr Glu Met Gln Ser Gln Met Gln Leu Ala Asp 
            100                 105                 110         


Gln Val Ser Leu Pro Gly His Cys Arg Glu Pro Pro Pro Trp Glu Asn 
        115                 120                 125             


Glu Ala Thr Glu Arg Ile Tyr His Phe Val Val Gly Gln Thr Val Tyr 
    130                 135                 140                 


Tyr Gln Cys Val Gln Gly Tyr Arg Ala Leu His Arg Gly Pro Ala Glu 
145                 150                 155                 160 


Ser Val Cys Lys Met Thr His Gly Lys Thr Arg Trp Thr Gln Pro Gln 
                165                 170                 175     


Leu Ile Cys Thr Gly Glu Val Asp Glu Gln Leu Tyr Phe Gln Gly Gly 
            180                 185                 190         


Ser Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu 
        195                 200                 205             


Ala Arg Ala His His His His His His 
    210                 215         


<210>  38
<211>  654
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cynomolgous IL-2R alpha subunit + Avi-tag + His-tag

<400>  38
atgggatgga gctgtatcat cctcttcttg gtagcaacag ctaccggtga gctctgtgac       60

gatgacccgc caaaaatcac acatgccaca ttcaaagcca tggcctacaa ggaaggaacc      120

atgttgaact gtgaatgcaa gagaggtttc cgcagaataa aaagcgggtc accctatatg      180

ctctgtacag gaaactctag ccactcgtcc tgggacaacc aatgtcaatg cacaagctct      240

gctgctcgga acacaacaaa acaagtgaca cctcaacctg aagaacagaa agaaagaaaa      300

accacagaaa tgcaaagtca aatgcagctg gcggaccaag tgagccttcc aggtcactgc      360

agggaacctc caccgtggga aaatgaagcc acagaaagaa tttatcattt cgtggtgggg      420

cagacggttt actaccagtg cgtccaggga tacagggctc tacacagagg tcctgctgag      480

agcgtctgca aaatgaccca cgggaagaca agatggaccc agccccagct catatgcaca      540

ggtgaagtcg acgaacagtt atattttcag ggcggctcag gcctgaacga catcttcgag      600

gcccagaaga tcgagtggca cgaggctcga gctcaccacc atcaccatca ctga            654


<210>  39
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  leader sequence

<400>  39

Met Asp Trp Thr Trp Arg Ile Leu Phe Leu Val Ala Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser 
            


<210>  40
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  leader sequence

<400>  40
atggactgga cctggagaat cctcttcttg gtggcagcag ccacaggagc ccactcc          57


<210>  41
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  leader sequence

<400>  41
atggactgga cctggaggat cctcttcttg gtggcagcag ccacaggagc ccactcc          57


<210>  42
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  leader sequence

<400>  42

Met Asp Met Arg Val Pro Ala Gln Leu Leu Gly Leu Leu Leu Leu Trp 
1               5                   10                  15      


Phe Pro Gly Ala Arg Cys 
            20          


<210>  43
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  leader sequence

<400>  43
atggacatga gggtccccgc tcagctcctg ggcctcctgc tgctctggtt cccaggtgcc       60

aggtgt                                                                  66


<210>  44
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  leader sequence

<400>  44

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser 
            


<210>  45
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  leader sequence

<400>  45
atgggatgga gctgtatcat cctcttcttg gtagcaacag ctaccggtgt gcattcc          57


<210>  46
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  leader sequence

<400>  46
atgggctggt cctgcatcat cctgtttctg gtggctaccg ccactggagt gcattcc          57


<210>  47
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  leader sequence

<400>  47
atgggctggt cctgcatcat cctgtttctg gtcgccacag ccaccggcgt gcactct          57


<210>  48
<211>  592
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DP47GS HC(Fc knob,P329G LALA)-IL2 N88D

<400>  48

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Gly Ser Gly Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro 
        115                 120                 125             


Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val 
    130                 135                 140                 


Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala 
145                 150                 155                 160 


Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly 
                165                 170                 175     


Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly 
            180                 185                 190         


Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys 
        195                 200                 205             


Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
    210                 215                 220                 


Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe Leu 
225                 230                 235                 240 


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
                245                 250                 255     


Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys 
            260                 265                 270         


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
        275                 280                 285             


Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
    290                 295                 300                 


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
305                 310                 315                 320 


Val Ser Asn Lys Ala Leu Gly Ala Pro Ile Glu Lys Thr Ile Ser Lys 
                325                 330                 335     


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Cys 
            340                 345                 350         


Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Trp Cys Leu Val Lys 
        355                 360                 365             


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
    370                 375                 380                 


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
385                 390                 395                 400 


Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln 
                405                 410                 415     


Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
            420                 425                 430         


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Gly Gly Gly Gly 
        435                 440                 445             


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Pro Ala Ser Ser 
    450                 455                 460                 


Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His Leu Leu Leu Asp Leu 
465                 470                 475                 480 


Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys Asn Pro Lys Leu Thr 
                485                 490                 495     


Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys Lys Ala Thr Glu Leu 
            500                 505                 510         


Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys Pro Leu Glu Glu Val 
        515                 520                 525             


Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu Arg Pro Arg Asp Leu 
    530                 535                 540                 


Ile Ser Asp Ile Asn Val Ile Val Leu Glu Leu Lys Gly Ser Glu Thr 
545                 550                 555                 560 


Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala Thr Ile Val Glu Phe 
                565                 570                 575     


Leu Asn Arg Trp Ile Thr Phe Ala Gln Ser Ile Ile Ser Thr Leu Thr 
            580                 585                 590         


<210>  49
<211>  1776
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DP47GS HC(Fc knob,P329G LALA)-IL2 N88D

<400>  49
gaggtgcaat tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc       60

tcctgtgcag cctccggatt cacctttagc agttatgcca tgagctgggt ccgccaggct      120

ccagggaagg ggctggagtg ggtctcagct attagtggta gtggtggtag cacatactac      180

gcagactccg tgaagggccg gttcaccatc tccagagaca attccaagaa cacgctgtat      240

ctgcagatga acagcctgag agccgaggac acggccgtat attactgtgc gaaaggcagc      300

ggatttgact actggggcca aggaaccctg gtcaccgtct cgagtgctag caccaagggc      360

ccatcggtct tccccctggc accctcctcc aagagcacct ctgggggcac agcggccctg      420

ggctgcctgg tcaaggacta cttccccgaa ccggtgacgg tgtcgtggaa ctcaggcgcc      480

ctgaccagcg gcgtgcacac cttcccggct gtcctacagt cctcaggact ctactccctc      540

agcagcgtgg tgaccgtgcc ctccagcagc ttgggcaccc agacctacat ctgcaacgtg      600

aatcacaagc ccagcaacac caaggtggac aagaaagttg agcccaaatc ttgtgacaaa      660

actcacacat gcccaccgtg cccagcacct gaagctgcag ggggaccgtc agtcttcctc      720

ttccccccaa aacccaagga caccctcatg atctcccgga cccctgaggt cacatgcgtg      780

gtggtggacg tgagccacga agaccctgag gtcaagttca actggtacgt ggacggcgtg      840

gaggtgcata atgccaagac aaagccgcgg gaggagcagt acaacagcac gtaccgtgtg      900

gtcagcgtcc tcaccgtcct gcaccaggac tggctgaatg gcaaggagta caagtgcaag      960

gtctccaaca aagccctcgg cgcccccatc gagaaaacca tctccaaagc caaagggcag     1020

ccccgagaac cacaggtgta caccctgccc ccatgccggg atgagctgac caagaaccag     1080

gtcagcctgt ggtgcctggt caaaggcttc tatcccagcg acatcgccgt ggagtgggag     1140

agcaatgggc agccggagaa caactacaag accacgcctc ccgtgctgga ctccgacggc     1200

tccttcttcc tctacagcaa gctcaccgtg gacaagagca ggtggcagca ggggaacgtc     1260

ttctcatgct ccgtgatgca tgaggctctg cacaaccact acacgcagaa gagcctctcc     1320

ctgtctccgg gtggcggcgg aggctccgga ggcggaggtt ctggaggcgg aggctccgca     1380

cctgcctcaa gttctacaaa gaaaacacag ctacaactgg agcatttact gctggattta     1440

cagatgattt tgaatggaat taataattac aagaatccca aactcaccag gatgctcaca     1500

tttaagtttt acatgcccaa gaaggccaca gaactgaaac atcttcagtg tctagaagaa     1560

gaactcaaac ctctggagga agtgctaaat ttagctcaaa gcaaaaactt tcacttaaga     1620

cccagggact taatcagcga tatcaacgta atagttctgg aactaaaggg atctgaaaca     1680

acattcatgt gtgaatatgc tgatgagaca gcaaccattg tagaatttct gaacagatgg     1740

attacctttg cccaaagcat catctcaaca ctgact                               1776


<210>  50
<211>  592
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DP47GS HC(Fc wt,P329G LALA)-IL2 N88D

<400>  50

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Gly Ser Gly Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro 
        115                 120                 125             


Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val 
    130                 135                 140                 


Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala 
145                 150                 155                 160 


Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly 
                165                 170                 175     


Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly 
            180                 185                 190         


Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys 
        195                 200                 205             


Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
    210                 215                 220                 


Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe Leu 
225                 230                 235                 240 


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
                245                 250                 255     


Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys 
            260                 265                 270         


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
        275                 280                 285             


Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
    290                 295                 300                 


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
305                 310                 315                 320 


Val Ser Asn Lys Ala Leu Gly Ala Pro Ile Glu Lys Thr Ile Ser Lys 
                325                 330                 335     


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
            340                 345                 350         


Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
        355                 360                 365             


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
    370                 375                 380                 


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
385                 390                 395                 400 


Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln 
                405                 410                 415     


Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
            420                 425                 430         


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Gly Gly Gly Gly 
        435                 440                 445             


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Pro Ala Ser Ser 
    450                 455                 460                 


Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His Leu Leu Leu Asp Leu 
465                 470                 475                 480 


Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys Asn Pro Lys Leu Thr 
                485                 490                 495     


Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys Lys Ala Thr Glu Leu 
            500                 505                 510         


Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys Pro Leu Glu Glu Val 
        515                 520                 525             


Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu Arg Pro Arg Asp Leu 
    530                 535                 540                 


Ile Ser Asp Ile Asn Val Ile Val Leu Glu Leu Lys Gly Ser Glu Thr 
545                 550                 555                 560 


Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala Thr Ile Val Glu Phe 
                565                 570                 575     


Leu Asn Arg Trp Ile Thr Phe Ala Gln Ser Ile Ile Ser Thr Leu Thr 
            580                 585                 590         


<210>  51
<211>  1776
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DP47GS HC(Fc wt,P329G LALA)-IL2 N88D

<400>  51
gaggtgcaat tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc       60

tcctgtgcag cctccggatt cacctttagc agttatgcca tgagctgggt ccgccaggct      120

ccagggaagg ggctggagtg ggtctcagct attagtggta gtggtggtag cacatactac      180

gcagactccg tgaagggccg gttcaccatc tccagagaca attccaagaa cacgctgtat      240

ctgcagatga acagcctgag agccgaggac acggccgtat attactgtgc gaaaggcagc      300

ggatttgact actggggcca aggaaccctg gtcaccgtct cgagtgctag caccaagggc      360

ccatcggtct tccccctggc accctcctcc aagagcacct ctgggggcac agcggccctg      420

ggctgcctgg tcaaggacta cttccccgaa ccggtgacgg tgtcgtggaa ctcaggcgcc      480

ctgaccagcg gcgtgcacac cttcccggct gtcctacagt cctcaggact ctactccctc      540

agcagcgtgg tgaccgtgcc ctccagcagc ttgggcaccc agacctacat ctgcaacgtg      600

aatcacaagc ccagcaacac caaggtggac aagaaagttg agcccaaatc ttgtgacaaa      660

actcacacat gcccaccgtg cccagcacct gaagctgcag ggggaccgtc agtcttcctc      720

ttccccccaa aacccaagga caccctcatg atctcccgga cccctgaggt cacatgcgtg      780

gtggtggacg tgagccacga agaccctgag gtcaagttca actggtacgt ggacggcgtg      840

gaggtgcata atgccaagac aaagccgcgg gaggagcagt acaacagcac gtaccgtgtg      900

gtcagcgtcc tcaccgtcct gcaccaggac tggctgaatg gcaaggagta caagtgcaag      960

gtctccaaca aagccctcgg cgcccccatc gagaaaacca tctccaaagc caaagggcag     1020

ccccgagaac cacaggtgta caccctgccc ccatcccggg atgagctgac caagaaccag     1080

gtcagcctga cctgcctggt caaaggcttc tatcccagcg acatcgccgt ggagtgggag     1140

agcaatgggc agccggagaa caactacaag accacgcctc ccgtgctgga ctccgacggc     1200

tccttcttcc tctacagcaa gctcaccgtg gacaagagca ggtggcagca ggggaacgtc     1260

ttctcatgct ccgtgatgca tgaggctctg cacaaccact acacgcagaa gagcctctcc     1320

ctgtctccgg gtggcggcgg aggctccgga ggcggaggtt ctggaggcgg aggctccgca     1380

cctgcctcaa gttctacaaa gaaaacacag ctacaactgg agcatttact gctggattta     1440

cagatgattt tgaatggaat taataattac aagaatccca aactcaccag gatgctcaca     1500

tttaagtttt acatgcccaa gaaggccaca gaactgaaac atcttcagtg tctagaagaa     1560

gaactcaaac ctctggagga agtgctaaat ttagctcaaa gcaaaaactt tcacttaaga     1620

cccagggact taatcagcga tatcaacgta atagttctgg aactaaaggg atctgaaaca     1680

acattcatgt gtgaatatgc tgatgagaca gcaaccattg tagaatttct gaacagatgg     1740

attacctttg cccaaagcat catctcaaca ctgact                               1776


<210>  52
<211>  592
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DP47GS HC(Fc wt, P329G LALA)-IL2 E95A

<400>  52

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Gly Ser Gly Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro 
        115                 120                 125             


Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val 
    130                 135                 140                 


Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala 
145                 150                 155                 160 


Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly 
                165                 170                 175     


Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly 
            180                 185                 190         


Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys 
        195                 200                 205             


Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
    210                 215                 220                 


Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe Leu 
225                 230                 235                 240 


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
                245                 250                 255     


Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys 
            260                 265                 270         


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
        275                 280                 285             


Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
    290                 295                 300                 


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
305                 310                 315                 320 


Val Ser Asn Lys Ala Leu Gly Ala Pro Ile Glu Lys Thr Ile Ser Lys 
                325                 330                 335     


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
            340                 345                 350         


Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
        355                 360                 365             


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
    370                 375                 380                 


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
385                 390                 395                 400 


Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln 
                405                 410                 415     


Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
            420                 425                 430         


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Gly Gly Gly Gly 
        435                 440                 445             


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Pro Ala Ser Ser 
    450                 455                 460                 


Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His Leu Leu Leu Asp Leu 
465                 470                 475                 480 


Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys Asn Pro Lys Leu Thr 
                485                 490                 495     


Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys Lys Ala Thr Glu Leu 
            500                 505                 510         


Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys Pro Leu Glu Glu Val 
        515                 520                 525             


Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu Arg Pro Arg Asp Leu 
    530                 535                 540                 


Ile Ser Asn Ile Asn Val Ile Val Leu Pro Leu Lys Gly Ser Glu Thr 
545                 550                 555                 560 


Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala Thr Ile Val Glu Phe 
                565                 570                 575     


Leu Asn Arg Trp Ile Thr Phe Ala Gln Ser Ile Ile Ser Thr Leu Thr 
            580                 585                 590         


<210>  53
<211>  1776
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DP47GS HC(Fc wt, P329G LALA)-IL2 E95A

<400>  53
gaggtgcaat tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc       60

tcctgtgcag cctccggatt cacctttagc agttatgcca tgagctgggt ccgccaggct      120

ccagggaagg ggctggagtg ggtctcagct attagtggta gtggtggtag cacatactac      180

gcagactccg tgaagggccg gttcaccatc tccagagaca attccaagaa cacgctgtat      240

ctgcagatga acagcctgag agccgaggac acggccgtat attactgtgc gaaaggcagc      300

ggatttgact actggggcca aggaaccctg gtcaccgtct cgagtgctag caccaagggc      360

ccatcggtct tccccctggc accctcctcc aagagcacct ctgggggcac agcggccctg      420

ggctgcctgg tcaaggacta cttccccgaa ccggtgacgg tgtcgtggaa ctcaggcgcc      480

ctgaccagcg gcgtgcacac cttcccggct gtcctacagt cctcaggact ctactccctc      540

agcagcgtgg tgaccgtgcc ctccagcagc ttgggcaccc agacctacat ctgcaacgtg      600

aatcacaagc ccagcaacac caaggtggac aagaaagttg agcccaaatc ttgtgacaaa      660

actcacacat gcccaccgtg cccagcacct gaagctgcag ggggaccgtc agtcttcctc      720

ttccccccaa aacccaagga caccctcatg atctcccgga cccctgaggt cacatgcgtg      780

gtggtggacg tgagccacga agaccctgag gtcaagttca actggtacgt ggacggcgtg      840

gaggtgcata atgccaagac aaagccgcgg gaggagcagt acaacagcac gtaccgtgtg      900

gtcagcgtcc tcaccgtcct gcaccaggac tggctgaatg gcaaggagta caagtgcaag      960

gtctccaaca aagccctcgg cgcccccatc gagaaaacca tctccaaagc caaagggcag     1020

ccccgagaac cacaggtgta caccctgccc ccatcccggg atgagctgac caagaaccag     1080

gtcagcctga cctgcctggt caaaggcttc tatcccagcg acatcgccgt ggagtgggag     1140

agcaatgggc agccggagaa caactacaag accacgcctc ccgtgctgga ctccgacggc     1200

tccttcttcc tctacagcaa gctcaccgtg gacaagagca ggtggcagca ggggaacgtc     1260

ttctcatgct ccgtgatgca tgaggctctg cacaaccact acacgcagaa gagcctctcc     1320

ctgtctccgg gtggcggcgg aggctccgga ggcggaggtt ctggaggcgg aggctccgca     1380

cctgcctcaa gttctacaaa gaaaacacag ctacaactgg agcatttact gctggattta     1440

cagatgattt tgaatggaat taataattac aagaatccca aactcaccag gatgctcaca     1500

tttaagtttt acatgcccaa gaaggccaca gaactgaaac atcttcagtg tctagaagaa     1560

gaactcaaac ctctggagga agtgctaaat ttagctcaaa gcaaaaactt tcacttaaga     1620

cccagggact taatcagcaa tatcaacgta atagttctgc cactaaaggg atctgaaaca     1680

acattcatgt gtgaatatgc tgatgagaca gcaaccattg tagaatttct gaacagatgg     1740

attacctttg cccaaagcat catctcaaca ctgact                               1776


<210>  54
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FOXP3 forward primer

<400>  54
tgtaaaacga cggccagttt tagaagttgt atgggggatg tt                          42


<210>  55
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FOXP3 reverse primer

<400>  55
caggaaacag ctatgaccaa aatatctacc ctcttctctt cctc                        44


<210>  56
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CTLA-4 forward primer

<400>  56
tgtaaaacga cggccagtgg gtttggttat gaaggagtat ga                          42


<210>  57
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CTLA-4 reverse primer

<400>  57
caggaaacag ctatgacctt cacttaattt ccactaaaaa taccc                       45


<210>  58
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2 N88D T3A C125A

<400>  58

Ala Pro Ala Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asp Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ala Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  59
<211>  399
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IL-2 N88D T3A C125A

<400>  59
gcacctgcct caagttctac aaagaaaaca cagctacaac tggagcattt actgctggat       60

ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc      120

acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa      180

gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta      240

agacccaggg acttaatcag cgatatcaac gtaatagttc tggaactaaa gggatctgaa      300

acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga      360

tggattacct ttgcccaaag catcatctca acactgact                             399


<210>  60
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2 N88D

<400>  60

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asp Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  61
<211>  399
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IL-2 N88D

<400>  61
gcacctactt caagttctac aaagaaaaca cagctacaac tggagcattt actgctggat       60

ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc      120

acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa      180

gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta      240

agacccaggg acttaatcag cgatatcaac gtaatagttc tggaactaaa gggatctgaa      300

acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga      360

tggattacct tttgtcaaag catcatctca acactgact                             399


<210>  62
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2 N88D C125A

<400>  62

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asp Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ala Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  63
<211>  399
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IL-2 N88D C125A

<400>  63
gcacctactt caagttctac aaagaaaaca cagctacaac tggagcattt actgctggat       60

ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc      120

acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa      180

gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta      240

agacccaggg acttaatcag cgatatcaac gtaatagttc tggaactaaa gggatctgaa      300

acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga      360

tggattacct ttgcccaaag catcatctca acactgact                             399


<210>  64
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2 N88D T3A

<400>  64

Ala Pro Ala Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asp Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  65
<211>  399
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IL-2 N88D T3A

<400>  65
gcacctgcct caagttctac aaagaaaaca cagctacaac tggagcattt actgctggat       60

ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc      120

acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa      180

gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta      240

agacccaggg acttaatcag cgatatcaac gtaatagttc tggaactaaa gggatctgaa      300

acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga      360

tggattacct tttgtcaaag catcatctca acactgact                             399


<210>  66
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (G4S)3 linker

<400>  66

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


