                         SEQUENCE LISTING

<110>  BASF SE
 
<120>  Improved microorganism for succinic acid production

<130>  0000075273WO01

<160>  32    

<170>  PatentIn version 3.3

<210>  1
<211>  1517
<212>  DNA
<213>  Basfia succiniciproducens


<220>
<221>  misc_feature
<222>  (1)..(1517)
<223>  16 S rDNA of strain DD1

<400>  1
tttgatcctg gctcagattg aacgctggcg gcaggcttaa cacatgcaag tcgaacggta     60

gcgggaggaa agcttgcttt ctttgccgac gagtggcgga cgggtgagta atgcttgggg    120

atctggctta tggaggggga taacgacggg aaactgtcgc taataccgcg taatatcttc    180

ggattaaagg gtgggacttt cgggccaccc gccataagat gagcccaagt gggattaggt    240

agttggtggg gtaaaggcct accaagccga cgatctctag ctggtctgag aggatgacca    300

gccacactgg aactgagaca cggtccagac tcctacggga ggcagcagtg gggaatattg    360

cacaatgggg ggaaccctga tgcagccatg ccgcgtgaat gaagaaggcc ttcgggttgt    420

aaagttcttt cggtgacgag gaaggtgttt gttttaatag gacaagcaat tgacgttaat    480

cacagaagaa gcaccggcta actccgtgcc agcagccgcg gtaatacgga gggtgcgagc    540

gttaatcgga ataactgggc gtaaagggca tgcaggcgga cttttaagtg agatgtgaaa    600

gccccgggct taacctggga attgcatttc agactgggag tctagagtac tttagggagg    660

ggtagaattc cacgtgtagc ggtgaaatgc gtagagatgt ggaggaatac cgaaggcgaa    720

ggcagcccct tgggaagata ctgacgctca tatgcgaaag cgtggggagc aaacaggatt    780

agataccctg gtagtccacg cggtaaacgc tgtcgatttg gggattgggc tttaggcctg    840

gtgctcgtag ctaacgtgat aaatcgaccg cctggggagt acggccgcaa ggttaaaact    900

caaatgaatt gacgggggcc cgcacaagcg gtggagcatg tggtttaatt cgatgcaacg    960

cgaagaacct tacctactct tgacatccag agaatcctgt agagatacgg gagtgccttc   1020

gggagctctg agacaggtgc tgcatggctg tcgtcagctc gtgttgtgaa atgttgggtt   1080

aagtcccgca acgagcgcaa cccttatcct ttgttgccag catgtaaaga tgggaactca   1140

aaggagactg ccggtgacaa accggaggaa ggtggggatg acgtcaagtc atcatggccc   1200

ttacgagtag ggctacacac gtgctacaat ggtgcataca gagggcggcg ataccgcgag   1260

gtagagcgaa tctcagaaag tgcatcgtag tccggattgg agtctgcaac tcgactccat   1320

gaagtcggaa tcgctagtaa tcgcaaatca gaatgttgcg gtgaatacgt tcccgggcct   1380

tgtacacacc gcccgtcaca ccatgggagt gggttgtacc agaagtagat agcttaacct   1440

tcgggggggg cgtttaccac ggtatgattc atgactgggg tgaagtcgta acaaggtaac   1500

cgtaggggaa cctgcgg                                                  1517


<210>  2
<211>  3008
<212>  DNA
<213>  Basfia succiniciproducens


<220>
<221>  misc_feature
<222>  (1)..(3008)
<223>  23 S rDNA of strain DD1

<400>  2
agtaataacg aacgacacag gtataagaat acttgaggtt gtatggttaa gtgactaagc     60

gtacaaggtg gatgccttgg caatcagagg cgaagaagga cgtgctaatc tgcgaaaagc    120

ttgggtgagt tgataagaag cgtctaaccc aagatatccg aatggggcaa cccagtagat    180

gaagaatcta ctatcaataa ccgaatccat aggttattga ggcaaaccgg gagaactgaa    240

acatctaagt accccgagga aaagaaatca accgagatta cgtcagtagc ggcgagcgaa    300

agcgtaagag ccggcaagtg atagcatgag gattagagga atcggctggg aagccgggcg    360

gcacagggtg atagccccgt acttgaaaat cattgtgtgg tactgagctt gcgagaagta    420

gggcgggaca cgagaaatcc tgtttgaaga aggggggacc atcctccaag gctaaatact    480

cctgattgac cgatagtgaa ccagtactgt gaaggaaagg cgaaaagaac cccggtgagg    540

ggagtgaaat agaacctgaa accttgtacg tacaagcagt gggagcccgc gagggtgact    600

gcgtaccttt tgtataatgg gtcagcgact tatattatgt agcgaggtta accgaatagg    660

ggagccgaag ggaaaccgag tcttaactgg gcgtcgagtt gcatgatata gacccgaaac    720

ccggtgatct agccatgggc aggttgaagg ttgggtaaca ctaactggag gaccgaaccg    780

actaatgttg aaaaattagc ggatgacctg tggctggggg tgaaaggcca atcaaaccgg    840

gagatagctg gttctccccg aaatctattt aggtagagcc ttatgtgaat accttcgggg    900

gtagagcact gtttcggcta gggggccatc ccggcttacc aacccgatgc aaactgcgaa    960

taccgaagag taatgcatag gagacacacg gcgggtgcta acgttcgtcg tggagaggga   1020

aacaacccag accgccagct aaggtcccaa agtttatatt aagtgggaaa cgaagtggga   1080

aggcttagac agctaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag   1140

ctcactagtc gagtcggcct gcgcggaaga tgtaacgggg ctcaaatata gcaccgaagc   1200

tgcggcatca ggcgtaagcc tgttgggtag gggagcgtcg tgtaagcgga agaaggtggt   1260

tcgagagggc tgctggacgt atcacgagtg cgaatgctga cataagtaac gataaaacgg   1320

gtgaaaaacc cgttcgccgg aagaccaagg gttcctgtcc aacgttaatc ggggcagggt   1380

gagtcggccc ctaaggcgag gctgaagagc gtagtcgatg ggaaacgggt taatattccc   1440

gtacttgtta taattgcgat gtggggacgg agtaggttag gttatcgacc tgttggaaaa   1500

ggtcgtttaa gttggtaggt ggagcgttta ggcaaatccg gacgcttatc aacaccgaga   1560

gatgatgacg aggcgctaag gtgccgaagt aaccgatacc acacttccag gaaaagccac   1620

taagcgtcag attataataa accgtactat aaaccgacac aggtggtcag gtagagaata   1680

ctcaggcgct tgagagaact cgggtgaagg aactaggcaa aatagcaccg taacttcggg   1740

agaaggtgcg ccggcgtaga ttgtagaggt atacccttga aggttgaacc ggtcgaagtg   1800

acccgctggc tgcaactgtt tattaaaaac acagcactct gcaaacacga aagtggacgt   1860

atagggtgtg atgcctgccc ggtgctggaa ggttaattga tggcgttatc gcaagagaag   1920

cgcctgatcg aagccccagt aaacggcggc cgtaactata acggtcctaa ggtagcgaaa   1980

ttccttgtcg ggtaagttcc gacctgcacg aatggcataa tgatggccag gctgtctcca   2040

cccgagactc agtgaaattg aaatcgccgt gaagatgcgg tgtacccgcg gctagacgga   2100

aagaccccgt gaacctttac tatagcttga cactgaacct tgaattttga tgtgtaggat   2160

aggtgggagg ctttgaagcg gtaacgccag ttatcgtgga gccatccttg aaataccacc   2220

ctttaacgtt tgatgttcta acgaagtgcc cggaacgggt actcggacag tgtctggtgg   2280

gtagtttgac tggggcggtc tcctcccaaa gagtaacgga ggagcacgaa ggtttgctaa   2340

tgacggtcgg acatcgtcag gttagtgcaa tggtataagc aagcttaact gcgagacgga   2400

caagtcgagc aggtgcgaaa gcaggtcata gtgatccggt ggttctgaat ggaagggcca   2460

tcgctcaacg gataaaaggt actccgggga taacaggctg ataccgccca agagttcata   2520

tcgacggcgg tgtttggcac ctcgatgtcg gctcatcaca tcctggggct gaagtaggtc   2580

ccaagggtat ggctgttcgc catttaaagt ggtacgcgag ctgggtttaa aacgtcgtga   2640

gacagtttgg tccctatctg ccgtgggcgt tggagaattg agaggggctg ctcctagtac   2700

gagaggaccg gagtggacgc atcactggtg ttccggttgt gtcgccagac gcattgccgg   2760

gtagctacat gcggaagaga taagtgctga aagcatctaa gcacgaaact tgcctcgaga   2820

tgagttctcc cagtatttaa tactgtaagg gttgttggag acgacgacgt agataggccg   2880

ggtgtgtaag cgttgcgaga cgttgagcta accggtacta attgcccgag aggcttagcc   2940

atacaacgct caagtgtttt tggtagtgaa agttattacg gaataagtaa gtagtcaggg   3000

aatcggct                                                            3008


<210>  3
<211>  1440
<212>  DNA
<213>  Basfia succiniciproducens


<220>
<221>  misc_feature
<222>  (1)..(1440)
<223>  nucleotide sequence of pykA-gene from strain DD1

<400>  3
atgtccagaa gattaagaag aacgaaaatc gtatgtacaa tggggcctgc aacagacaaa     60

ggcaataatt tagaaaaaat cattgctgcc ggtgcaaacg ttgtacgtat gaacttctcc    120

cacggtacgc ccgaagatca tatcggtcgt gctgaaaaag tacgtgaaat cgctcataaa    180

ttaggtaaac acgtagcaat cttaggtgac ttacaaggcc ctaaaatccg tgtttctact    240

tttaaagaag gcaaaatttt cttaaatatc ggtgataaat tcattttaga cgcagagatg    300

cctaaaggtg aaggtaacca ggaagcggtt ggtttagact ataaaacatt accgcaagat    360

gtggttccgg gcgatatctt attattagat gacggtcgag ttcaattgaa agtattggca    420

accgaaggtg caaaagtatt caccgaagta acggtcggtg gcccactatc aaataataaa    480

ggcattaaca aattaggcgg cggtttatct gccgatgcat taaccgaaaa agataaagcg    540

gatatcatta ctgcggcgcg tatcggtgtg gattaccttg ccgtatcttt cccgcgttca    600

agcgcggatt taaactacgc ccgtcaatta gcaaaagatg cgggcttgga tgcgaaaatc    660

gttgcgaaag tagaacgtgc cgaaacagtt gaaacggacg aagcaatgga cgatatcatc    720

aatgcggcgg acgtaatcat ggttgcgcgc ggtgacttag gtgttgaaat cggtgatccg    780

gaattagtcg gtgttcagaa aaaattaatc cgtcgttcac gtcagttaaa tcgtgttgtt    840

attaccgcaa ctcaaatgat ggaatcaatg attagtaatc ctatgccgac tcgtgcggaa    900

gtaatggacg tagctaacgc agtattggac ggtaccgatg cggtaatgct ttctgctgaa    960

accgcggctg gtcaatatcc ggcggaaact gttgcggcga tggcgaaagt tgcgttaggt   1020

gcggagaaaa tgccaagcat taatgtgtct aaacaccgta tgaacgttca attcgagtct   1080

attgaagaat ctgttgcgat gtctgcaatg tatgcggcaa accacatgag aggcgtagcg   1140

gcgattatca cattaacaag tagcggtcgt actgctcgtt taatgtctcg cattagttcc   1200

ggtttaccaa tctttgcatt gtcacgtaac gaatctacat taaacttatg cgcattatat   1260

cgtggtgtga caccggttca ttttgataaa gacagccgta cctcagaagg tgcgacagcg   1320

gcggttcaat tattaaaaga cgaaggtttc ttagtgtctg gcgatttagt gttattaact   1380

cagggcgacg caagcagttc tagcggtact aacctttgcc gtacattgat tgttgaataa   1440


<210>  4
<211>  479
<212>  PRT
<213>  Basfia succiniciproducens


<220>
<221>  SITE
<222>  (1)..(479)
<223>  amino acid sequence pf PykA from strain DD1

<400>  4

Met Ser Arg Arg Leu Arg Arg Thr Lys Ile Val Cys Thr Met Gly Pro 
1               5                   10                  15      


Ala Thr Asp Lys Gly Asn Asn Leu Glu Lys Ile Ile Ala Ala Gly Ala 
            20                  25                  30          


Asn Val Val Arg Met Asn Phe Ser His Gly Thr Pro Glu Asp His Ile 
        35                  40                  45              


Gly Arg Ala Glu Lys Val Arg Glu Ile Ala His Lys Leu Gly Lys His 
    50                  55                  60                  


Val Ala Ile Leu Gly Asp Leu Gln Gly Pro Lys Ile Arg Val Ser Thr 
65                  70                  75                  80  


Phe Lys Glu Gly Lys Ile Phe Leu Asn Ile Gly Asp Lys Phe Ile Leu 
                85                  90                  95      


Asp Ala Glu Met Pro Lys Gly Glu Gly Asn Gln Glu Ala Val Gly Leu 
            100                 105                 110         


Asp Tyr Lys Thr Leu Pro Gln Asp Val Val Pro Gly Asp Ile Leu Leu 
        115                 120                 125             


Leu Asp Asp Gly Arg Val Gln Leu Lys Val Leu Ala Thr Glu Gly Ala 
    130                 135                 140                 


Lys Val Phe Thr Glu Val Thr Val Gly Gly Pro Leu Ser Asn Asn Lys 
145                 150                 155                 160 


Gly Ile Asn Lys Leu Gly Gly Gly Leu Ser Ala Asp Ala Leu Thr Glu 
                165                 170                 175     


Lys Asp Lys Ala Asp Ile Ile Thr Ala Ala Arg Ile Gly Val Asp Tyr 
            180                 185                 190         


Leu Ala Val Ser Phe Pro Arg Ser Ser Ala Asp Leu Asn Tyr Ala Arg 
        195                 200                 205             


Gln Leu Ala Lys Asp Ala Gly Leu Asp Ala Lys Ile Val Ala Lys Val 
    210                 215                 220                 


Glu Arg Ala Glu Thr Val Glu Thr Asp Glu Ala Met Asp Asp Ile Ile 
225                 230                 235                 240 


Asn Ala Ala Asp Val Ile Met Val Ala Arg Gly Asp Leu Gly Val Glu 
                245                 250                 255     


Ile Gly Asp Pro Glu Leu Val Gly Val Gln Lys Lys Leu Ile Arg Arg 
            260                 265                 270         


Ser Arg Gln Leu Asn Arg Val Val Ile Thr Ala Thr Gln Met Met Glu 
        275                 280                 285             


Ser Met Ile Ser Asn Pro Met Pro Thr Arg Ala Glu Val Met Asp Val 
    290                 295                 300                 


Ala Asn Ala Val Leu Asp Gly Thr Asp Ala Val Met Leu Ser Ala Glu 
305                 310                 315                 320 


Thr Ala Ala Gly Gln Tyr Pro Ala Glu Thr Val Ala Ala Met Ala Lys 
                325                 330                 335     


Val Ala Leu Gly Ala Glu Lys Met Pro Ser Ile Asn Val Ser Lys His 
            340                 345                 350         


Arg Met Asn Val Gln Phe Glu Ser Ile Glu Glu Ser Val Ala Met Ser 
        355                 360                 365             


Ala Met Tyr Ala Ala Asn His Met Arg Gly Val Ala Ala Ile Ile Thr 
    370                 375                 380                 


Leu Thr Ser Ser Gly Arg Thr Ala Arg Leu Met Ser Arg Ile Ser Ser 
385                 390                 395                 400 


Gly Leu Pro Ile Phe Ala Leu Ser Arg Asn Glu Ser Thr Leu Asn Leu 
                405                 410                 415     


Cys Ala Leu Tyr Arg Gly Val Thr Pro Val His Phe Asp Lys Asp Ser 
            420                 425                 430         


Arg Thr Ser Glu Gly Ala Thr Ala Ala Val Gln Leu Leu Lys Asp Glu 
        435                 440                 445             


Gly Phe Leu Val Ser Gly Asp Leu Val Leu Leu Thr Gln Gly Asp Ala 
    450                 455                 460                 


Ser Ser Ser Ser Gly Thr Asn Leu Cys Arg Thr Leu Ile Val Glu 
465                 470                 475                 


<210>  5
<211>  4285
<212>  DNA
<213>  artificial sequence

<220>
<223>  complete nucleotide sequence of plasmid pSacB

<400>  5
tcgagaggcc tgacgtcggg cccggtacca cgcgtcatat gactagttcg gacctaggga     60

tatcgtcgac atcgatgctc ttctgcgtta attaacaatt gggatcctct agactccata    120

ggccgctttc ctggctttgc ttccagatgt atgctctcct ccggagagta ccgtgacttt    180

attttcggca caaatacagg ggtcgatgga taaatacggc gatagtttcc tgacggatga    240

tccgtatgta ccggcggaag acaagctgca aacctgtcag atggagattg atttaatggc    300

ggatgtgctg agagcaccgc cccgtgaatc cgcagaactg atccgctatg tgtttgcgga    360

tgattggccg gaataaataa agccgggctt aatacagatt aagcccgtat agggtattat    420

tactgaatac caaacagctt acggaggacg gaatgttacc cattgagaca accagactgc    480

cttctgatta ttaatatttt tcactattaa tcagaaggaa taaccatgaa ttttacccgg    540

attgacctga atacctggaa tcgcagggaa cactttgccc tttatcgtca gcagattaaa    600

tgcggattca gcctgaccac caaactcgat attaccgctt tgcgtaccgc actggcggag    660

acaggttata agttttatcc gctgatgatt tacctgatct cccgggctgt taatcagttt    720

ccggagttcc ggatggcact gaaagacaat gaacttattt actgggacca gtcagacccg    780

gtctttactg tctttcataa agaaaccgaa acattctctg cactgtcctg ccgttatttt    840

ccggatctca gtgagtttat ggcaggttat aatgcggtaa cggcagaata tcagcatgat    900

accagattgt ttccgcaggg aaatttaccg gagaatcacc tgaatatatc atcattaccg    960

tgggtgagtt ttgacgggat ttaacctgaa catcaccgga aatgatgatt attttgcccc   1020

ggtttttacg atggcaaagt ttcagcagga aggtgaccgc gtattattac ctgtttctgt   1080

acaggttcat catgcagtct gtgatggctt tcatgcagca cggtttatta atacacttca   1140

gctgatgtgt gataacatac tgaaataaat taattaattc tgtatttaag ccaccgtatc   1200

cggcaggaat ggtggctttt tttttatatt ttaaccgtaa tctgtaattt cgtttcagac   1260

tggttcagga tgagctcgct tggactcctg ttgatagatc cagtaatgac ctcagaactc   1320

catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcca   1380

agcactagcg gcgcgccggc cggcccggtg tgaaataccg cacagatgcg taaggagaaa   1440

ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg   1500

gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg   1560

ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa   1620

ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg   1680

acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc   1740

tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc   1800

ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc   1860

ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg   1920

ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc   1980

actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga   2040

gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc   2100

tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac   2160

caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg   2220

atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc   2280

acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa   2340

ggccggccgc ggccgccatc ggcattttct tttgcgtttt tatttgttaa ctgttaattg   2400

tccttgttca aggatgctgt ctttgacaac agatgttttc ttgcctttga tgttcagcag   2460

gaagctcggc gcaaacgttg attgtttgtc tgcgtagaat cctctgtttg tcatatagct   2520

tgtaatcacg acattgtttc ctttcgcttg aggtacagcg aagtgtgagt aagtaaaggt   2580

tacatcgtta ggatcaagat ccatttttaa cacaaggcca gttttgttca gcggcttgta   2640

tgggccagtt aaagaattag aaacataacc aagcatgtaa atatcgttag acgtaatgcc   2700

gtcaatcgtc atttttgatc cgcgggagtc agtgaacagg taccatttgc cgttcatttt   2760

aaagacgttc gcgcgttcaa tttcatctgt tactgtgtta gatgcaatca gcggtttcat   2820

cacttttttc agtgtgtaat catcgtttag ctcaatcata ccgagagcgc cgtttgctaa   2880

ctcagccgtg cgttttttat cgctttgcag aagtttttga ctttcttgac ggaagaatga   2940

tgtgcttttg ccatagtatg ctttgttaaa taaagattct tcgccttggt agccatcttc   3000

agttccagtg tttgcttcaa atactaagta tttgtggcct ttatcttcta cgtagtgagg   3060

atctctcagc gtatggttgt cgcctgagct gtagttgcct tcatcgatga actgctgtac   3120

attttgatac gtttttccgt caccgtcaaa gattgattta taatcctcta caccgttgat   3180

gttcaaagag ctgtctgatg ctgatacgtt aacttgtgca gttgtcagtg tttgtttgcc   3240

gtaatgttta ccggagaaat cagtgtagaa taaacggatt tttccgtcag atgtaaatgt   3300

ggctgaacct gaccattctt gtgtttggtc ttttaggata gaatcatttg catcgaattt   3360

gtcgctgtct ttaaagacgc ggccagcgtt tttccagctg tcaatagaag tttcgccgac   3420

tttttgatag aacatgtaaa tcgatgtgtc atccgcattt ttaggatctc cggctaatgc   3480

aaagacgatg tggtagccgt gatagtttgc gacagtgccg tcagcgtttt gtaatggcca   3540

gctgtcccaa acgtccaggc cttttgcaga agagatattt ttaattgtgg acgaatcaaa   3600

ttcagaaact tgatattttt catttttttg ctgttcaggg atttgcagca tatcatggcg   3660

tgtaatatgg gaaatgccgt atgtttcctt atatggcttt tggttcgttt ctttcgcaaa   3720

cgcttgagtt gcgcctcctg ccagcagtgc ggtagtaaag gttaatactg ttgcttgttt   3780

tgcaaacttt ttgatgttca tcgttcatgt ctcctttttt atgtactgtg ttagcggtct   3840

gcttcttcca gccctcctgt ttgaagatgg caagttagtt acgcacaata aaaaaagacc   3900

taaaatatgt aaggggtgac gccaaagtat acactttgcc ctttacacat tttaggtctt   3960

gcctgcttta tcagtaacaa acccgcgcga tttacttttc gacctcattc tattagactc   4020

tcgtttggat tgcaactggt ctattttcct cttttgtttg atagaaaatc ataaaaggat   4080

ttgcagacta cgggcctaaa gaactaaaaa atctatctgt ttcttttcat tctctgtatt   4140

ttttatagtt tctgttgcat gggcataaag ttgccttttt aatcacaatt cagaaaatat   4200

cataatatct catttcacta aataatagtg aacggcaggt atatgtgatg ggttaaaaag   4260

gatcggcggc cgctcgattt aaatc                                         4285


<210>  6
<211>  7074
<212>  DNA
<213>  artificial sequence

<220>
<223>  complete nucleotide sequence of plasmid pSacB_delta_ldhA

<400>  6
tcgagaggcc tgacgtcggg cccggtacca cgcgtcatat gactagttcg gacctaggga     60

tgggtcagcc tgaacgaacc gcacttgtat gtaggtagtt ttgaccgccc gaatattcgt    120

tataccttgg tggaaaaatt caaaccgatg gagcaattat acaattttgt ggcggcgcaa    180

aaaggtaaaa gcggtatcgt ctattgcaac agccgtagca aagtggagcg cattgcggaa    240

gccctgaaga aaagaggcat ttccgcagcc gcttatcatg cgggcatgga gccgtcgcag    300

cgggaagcgg tgcaacaggc gtttcaacgg gataatattc aagtggtggt ggcgaccatt    360

gcttttggta tggggatcaa caaatctaat gtgcgttttg tggcgcattt tgatttatct    420

cgcagcattg aggcgtatta tcaggaaacc gggcgcgcgg ggcgggacga cctgccggcg    480

gaagcggtac tgttttacga gccggcggat tatgcctggt tgcataaaat tttattggaa    540

gagccggaaa gcccgcaacg ggatattaaa cggcataagc tggaagccat cggcgaattt    600

gccgaaagcc agacctgccg tcgtttagtg ctgttaaatt atttcggcga aaaccgccaa    660

acgccatgta ataactgtga tatctgcctc gatccgccga aaaaatatga cggattatta    720

gacgcgcaga aaatcctttc gaccatttat cgcaccgggc aacgtttcgg cacgcaatac    780

gtaatcggcg taatgcgcgg tttgcagaat cagaaaataa aagaaaatca acatgatgag    840

ttgaaagtct acggaattgg caaagataaa agcaaagaat actggcaatc ggtaattcgt    900

cagctgattc atttgggctt tgtgcaacaa atcatcagcg atttcggcat ggggaccaga    960

ttacagctca ccgaaagcgc gcgtcccgtg ctgcgcggcg aagtgtcttt ggaactggcc   1020

atgccgagat tatcttccat taccatggta caggctccgc aacgcaatgc ggtaaccaac   1080

tacgacaaag atttatttgc ccgcctgcgt ttcctgcgca aacagattgc cgacaaagaa   1140

aacattccgc cttatattgt gttcagtgac gcgaccttgc aggaaatgtc gttgtatcag   1200

ccgaccagca aagtggaaat gctgcaaatc aacggtgtcg gcgccatcaa atggcagcgc   1260

ttcggacagc cttttatggc gattattaaa gaacatcagg ctttgcgtaa agcgggtaag   1320

aatccgttgg aattgcaatc ttaaaatttt taactttttg accgcacttt taaggttagc   1380

aaattccaat aaaaagtgcg gtgggttttc gggaattttt aacgcgctga tttcctcgtc   1440

ttttcaattt yttcgyctcc atttgttcgg yggttgccgg atcctttctt gactgagatc   1500

cataagagag tagaatagcg ccgcttatat ttttaatagc gtacctaatc gggtacgctt   1560

tttttatgcg gaaaatccat atttttctac cgcacttttt ctttaaagat ttatacttaa   1620

gtctgtttga ttcaatttat ttggaggttt tatgcaacac attcaactgg ctcccgattt   1680

aacattcagt cgcttaattc aaggattctg gcggttaaaa agctggcgga aatcgccgca   1740

ggaattgctt acattcgtta agcaaggatt agaattaggc gttgatacgc tggatcatgc   1800

cgcttgttac ggggctttta cttccgaggc ggaattcgga cgggcgctgg cgctggataa   1860

atccttgcgc gcacagctta ctttggtgac caaatgcggg attttgtatc ctaatgaaga   1920

attacccgat ataaaatccc atcactatga caacagctac cgccatatta tgtggtcggc   1980

gcaacgttcc attgaaaaac tgcaatgcga ctatttagat gtattgctga ttcaccgwct   2040

ttctccctgt gcggatcccg aacaaatcgc gcgggctttt gatgaacttt atcaaaccgg   2100

raaagtacgt tatttcgggg tatctaacta tacgccggct aagttcgcca tgttgcaatc   2160

ttatgtgaat cagccgttaa tcactaatca aattgagatt tcgcctcttc atcgtcaggc   2220

ttttgatgac ggtaccctgg attttttact ggaaaaacgt attcaaccga tggcatggtc   2280

gccacttgcc ggcggtcgtt tattcaatca ggatgagaac agtcgggcgg tgcaaaaaac   2340

attactcgaa atcggtgaaa cgaaaggaga aacccgttta gatacattgg cttatgcctg   2400

gttattggcg catccggcaa aaattatgcc ggttatgggg tccggtaaaa ttgaacgggt   2460

aaaaagcgcg gcggatgcgt tacgaatttc cttcactgag gaagaatgga ttaaggttta   2520

tgttgccgca cagggacggg atattccgta acatcatccg tctaatcctg cgtatctggg   2580

gaaagatgcg tcatcgtaag aggtctataa tattcgtcgt tttgataagg gtgccatatc   2640

cggcacccgt taaaatcaca ttgcgttcgc aacaaaatta ttccttacga atagcattca   2700

cctcttttaa cagatgttga atatccgtat cggcaaaaat atcctctata tttgcggtta   2760

aacggcgccg ccagttagca tattgagtgc tggttcccgg aatattgacg ggttcggtca   2820

taccgagcca gtcttcaggt tggaatcccc atcgtcgaca tcgatgctct tctgcgttaa   2880

ttaacaattg ggatcctcta gactccatag gccgctttcc tggctttgct tccagatgta   2940

tgctctcctc cggagagtac cgtgacttta ttttcggcac aaatacaggg gtcgatggat   3000

aaatacggcg atagtttcct gacggatgat ccgtatgtac cggcggaaga caagctgcaa   3060

acctgtcaga tggagattga tttaatggcg gatgtgctga gagcaccgcc ccgtgaatcc   3120

gcagaactga tccgctatgt gtttgcggat gattggccgg aataaataaa gccgggctta   3180

atacagatta agcccgtata gggtattatt actgaatacc aaacagctta cggaggacgg   3240

aatgttaccc attgagacaa ccagactgcc ttctgattat taatattttt cactattaat   3300

cagaaggaat aaccatgaat tttacccgga ttgacctgaa tacctggaat cgcagggaac   3360

actttgccct ttatcgtcag cagattaaat gcggattcag cctgaccacc aaactcgata   3420

ttaccgcttt gcgtaccgca ctggcggaga caggttataa gttttatccg ctgatgattt   3480

acctgatctc ccgggctgtt aatcagtttc cggagttccg gatggcactg aaagacaatg   3540

aacttattta ctgggaccag tcagacccgg tctttactgt ctttcataaa gaaaccgaaa   3600

cattctctgc actgtcctgc cgttattttc cggatctcag tgagtttatg gcaggttata   3660

atgcggtaac ggcagaatat cagcatgata ccagattgtt tccgcaggga aatttaccgg   3720

agaatcacct gaatatatca tcattaccgt gggtgagttt tgacgggatt taacctgaac   3780

atcaccggaa atgatgatta ttttgccccg gtttttacga tggcaaagtt tcagcaggaa   3840

ggtgaccgcg tattattacc tgtttctgta caggttcatc atgcagtctg tgatggcttt   3900

catgcagcac ggtttattaa tacacttcag ctgatgtgtg ataacatact gaaataaatt   3960

aattaattct gtatttaagc caccgtatcc ggcaggaatg gtggcttttt ttttatattt   4020

taaccgtaat ctgtaatttc gtttcagact ggttcaggat gagctcgctt ggactcctgt   4080

tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc   4140

gccgggcgtt ttttattggt gagaatccaa gcactagcgg cgcgccggcc ggcccggtgt   4200

gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg   4260

ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag   4320

gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa   4380

ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc   4440

cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca   4500

ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg   4560

accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct   4620

catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt   4680

gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag   4740

tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc   4800

agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac   4860

actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga   4920

gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc   4980

aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg   5040

gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca   5100

aaaaggatct tcacctagat ccttttaaag gccggccgcg gccgccatcg gcattttctt   5160

ttgcgttttt atttgttaac tgttaattgt ccttgttcaa ggatgctgtc tttgacaaca   5220

gatgttttct tgcctttgat gttcagcagg aagctcggcg caaacgttga ttgtttgtct   5280

gcgtagaatc ctctgtttgt catatagctt gtaatcacga cattgtttcc tttcgcttga   5340

ggtacagcga agtgtgagta agtaaaggtt acatcgttag gatcaagatc catttttaac   5400

acaaggccag ttttgttcag cggcttgtat gggccagtta aagaattaga aacataacca   5460

agcatgtaaa tatcgttaga cgtaatgccg tcaatcgtca tttttgatcc gcgggagtca   5520

gtgaacaggt accatttgcc gttcatttta aagacgttcg cgcgttcaat ttcatctgtt   5580

actgtgttag atgcaatcag cggtttcatc acttttttca gtgtgtaatc atcgtttagc   5640

tcaatcatac cgagagcgcc gtttgctaac tcagccgtgc gttttttatc gctttgcaga   5700

agtttttgac tttcttgacg gaagaatgat gtgcttttgc catagtatgc tttgttaaat   5760

aaagattctt cgccttggta gccatcttca gttccagtgt ttgcttcaaa tactaagtat   5820

ttgtggcctt tatcttctac gtagtgagga tctctcagcg tatggttgtc gcctgagctg   5880

tagttgcctt catcgatgaa ctgctgtaca ttttgatacg tttttccgtc accgtcaaag   5940

attgatttat aatcctctac accgttgatg ttcaaagagc tgtctgatgc tgatacgtta   6000

acttgtgcag ttgtcagtgt ttgtttgccg taatgtttac cggagaaatc agtgtagaat   6060

aaacggattt ttccgtcaga tgtaaatgtg gctgaacctg accattcttg tgtttggtct   6120

tttaggatag aatcatttgc atcgaatttg tcgctgtctt taaagacgcg gccagcgttt   6180

ttccagctgt caatagaagt ttcgccgact ttttgataga acatgtaaat cgatgtgtca   6240

tccgcatttt taggatctcc ggctaatgca aagacgatgt ggtagccgtg atagtttgcg   6300

acagtgccgt cagcgttttg taatggccag ctgtcccaaa cgtccaggcc ttttgcagaa   6360

gagatatttt taattgtgga cgaatcaaat tcagaaactt gatatttttc atttttttgc   6420

tgttcaggga tttgcagcat atcatggcgt gtaatatggg aaatgccgta tgtttcctta   6480

tatggctttt ggttcgtttc tttcgcaaac gcttgagttg cgcctcctgc cagcagtgcg   6540

gtagtaaagg ttaatactgt tgcttgtttt gcaaactttt tgatgttcat cgttcatgtc   6600

tcctttttta tgtactgtgt tagcggtctg cttcttccag ccctcctgtt tgaagatggc   6660

aagttagtta cgcacaataa aaaaagacct aaaatatgta aggggtgacg ccaaagtata   6720

cactttgccc tttacacatt ttaggtcttg cctgctttat cagtaacaaa cccgcgcgat   6780

ttacttttcg acctcattct attagactct cgtttggatt gcaactggtc tattttcctc   6840

ttttgtttga tagaaaatca taaaaggatt tgcagactac gggcctaaag aactaaaaaa   6900

tctatctgtt tcttttcatt ctctgtattt tttatagttt ctgttgcatg ggcataaagt   6960

tgccttttta atcacaattc agaaaatatc ataatatctc atttcactaa ataatagtga   7020

acggcaggta tatgtgatgg gttaaaaagg atcggcggcc gctcgattta aatc         7074


<210>  7
<211>  7183
<212>  DNA
<213>  artificial sequence

<220>
<223>  complete nucleotide sequence of plasmid pSacB_delta_pflA

<400>  7
tcgagtcaat gcggatttga cttatgatgt ggcaaacaac cgatttccga ttattactac     60

acgtaaaagt tattggaaag cggcgattgc ggagtttctg ggttatatcc gcggctacga    120

taatgcggcg gatttccgta aattaggagc aaaaacctgg gatgccaacg ctaatgaaaa    180

tcaggtatgg ctgaataacc ctcatcgcaa aggcaccgac gacatggggc gcgtttacgg    240

cgtacagggc agagcctggc gtaagcctaa cggcgaaacc gttgatcaat tacgcaaaat    300

tgtcaacaat ttaagtcgcg gcattgatga tcgcggcgaa attctgacct ttttaaaccc    360

gggcgaattc gatctcggtt gtctgcgccc ttgtatgtac aatcacacgt tttctttgct    420

gggcgatacg ctttatttaa ccagttatca acgctcctgt gacgtacctt taggcttgaa    480

tttcaatcaa attcaagtat ttacattctt agctttaatg gcgcagatta ccggtaaaaa    540

agccggtcag gcatatcaca aaatcgtcaa tgcgcatatt tacgaagacc agctggaact    600

aatgcgcgac gtgcagttaa aacgcgaacc gttcccgtcg ccaaaactgg aaattaatcc    660

ggacattaaa acccttgaag atttagaaac ctgggtaacc atggatgatt tcaacgtcgt    720

tggttaccaa tgccacgaac cgataaaata tccgttctcg gtataaaccg acaaaagtgc    780

ggtcaaaaat ttaatatttt catctgttat agaaaatatt tttcaacata aaatctaggg    840

atgcctgttt ggcgtccgta aatacgcaga aaaatattaa atttttgacc gcactttttt    900

catctcaatt aacagcctga taattcttat ggatcaacaa attagctttg acgaaaaaat    960

gatgaatcga gctcttttcc ttgccgacaa ggcggaagct ttaggggaaa ttcccgtagg   1020

tgccgtattg gtggatgaac ggggcaatat cattggtgaa ggctggaacc tctctattgt   1080

gaactcggat cccaccgccc atgccgaaat tattgcgttg cgtaacgccg cgcagaaaat   1140

ccaaaattac cgcctgctca ataccacttt atacgtgact ttagaaccct gcaccatgtg   1200

cgccggcgcg attttacaca gccgaatcaa acgcttggta ttcggggcgt ccgattacaa   1260

aaccggtgcg gtgggttcca gatttcattt ttttgaggat tataaaatga atcatggggt   1320

tgagatcaca agcggtgtct tacaggatca atgcagtcag aagttaagcc gctttttcca   1380

aaagcgcagg gaacagaaaa aacaacaaaa agctaccgca cttttacaac acccccggct   1440

taactcctct gaaaaatagt gacaaaaaaa ccgtcataat gtttacgacg gtttttttat   1500

ttcttaatat gcccttaaat aatcaacaaa atatagcaag aagattatag caaagaattt   1560

cgtttttttc agagaatagt caaatcttcg caaaaaacta ccgcactttt atccgcttta   1620

atcaggggaa ttaaaacaaa aaaattccgc ctattgaggc ggaatttatt aagcaataag   1680

acaaactctc aattacattg attgtgtaaa cgtacgagtg atgacgtctt gttgttgctc   1740

tttagttaat gagttgaaac gaaccgcgta acctgaaaca cgaatggtta attgcgggta   1800

tttttccgga ttttccatcg cgtctaacaa catttcacgg ttaagaacgt taacattcaa   1860

gtgttgaccg ccttccactg tcgcttcatg atggaaataa ccgtccatta aaccggcaag   1920

gttgcgtttt tgcgcttcgt catctttacc taatgcgttc ggtacgatag agaaggtata   1980

tgaaataccg tctttcgcgt aagcgaacgg aagtttagcc acagaagtaa gtgaagcaac   2040

cgcacctttt tggtcacgac cgtgcattgg gtttgcaccc ggtccgaatg gcgcgcctgc   2100

tcgacgaccg tccggagtat taccggtttt cttaccgtat accacgttag aagtgatagt   2160

caggatagat tgtgtcggag ttgcgttgcg gtaagttttg tgtttttgaa cttttttcat   2220

gaaacgttca actaagtcta ccgctaaatc atcaacacgc ggatcattgt taccgaattg   2280

cggatattcg ccttcaattt cgaagtcgat agcaacattc gaggccacga cattaccgtc   2340

tttatctttg atgtcgccgc gaatcggttt aactttcgca tatttgattg cggataatga   2400

gtccgcagcc acggaaagac ccgcgatacc gcaagccatt gtacggaata cgtcgcgatc   2460

gtggaacgcc atcaatgccg cttcatatgc atatttatcg tgcatgaagt ggatgatgtt   2520

caatgcggtt acatattgag tcgccaacca gtccatgaaa ctgtccatac gttcgattac   2580

ggtatcgaaa ttcaatactt cgtctgtaat cggcgcagtt ttaggaccga cttgcatacc   2640

atttttctca tcgataccgc cgttaattgc gtataacata gttttagcta agtttgcgcg   2700

cgcaccgaag aattgcattt gtttacctac gaccatcggt gatacgcagc atgcgattgc   2760

atagtcatcg ttgttgaagt caggacgcat taagtcatca ttttcgtatt gtacggagga   2820

agtatcaata gatactttcg cacagaaacg tttgaacgct tcaggtaatt gttcggacca   2880

aagaatagtt aagtttggtt ccggagaagt acccatagtg tataaagtat gtaatacgcg   2940

gaagctgttt ttagttacca acggacgacc gtctaagccc ataccggcga tagtttcggt   3000

tgccctctag actccatagg ccgctttcct ggctttgctt ccagatgtat gctctcctcc   3060

ggagagtacc gtgactttat tttcggcaca aatacagggg tcgatggata aatacggcga   3120

tagtttcctg acggatgatc cgtatgtacc ggcggaagac aagctgcaaa cctgtcagat   3180

ggagattgat ttaatggcgg atgtgctgag agcaccgccc cgtgaatccg cagaactgat   3240

ccgctatgtg tttgcggatg attggccgga ataaataaag ccgggcttaa tacagattaa   3300

gcccgtatag ggtattatta ctgaatacca aacagcttac ggaggacgga atgttaccca   3360

ttgagacaac cagactgcct tctgattatt aatatttttc actattaatc agaaggaata   3420

accatgaatt ttacccggat tgacctgaat acctggaatc gcagggaaca ctttgccctt   3480

tatcgtcagc agattaaatg cggattcagc ctgaccacca aactcgatat taccgctttg   3540

cgtaccgcac tggcggagac aggttataag ttttatccgc tgatgattta cctgatctcc   3600

cgggctgtta atcagtttcc ggagttccgg atggcactga aagacaatga acttatttac   3660

tgggaccagt cagacccggt ctttactgtc tttcataaag aaaccgaaac attctctgca   3720

ctgtcctgcc gttattttcc ggatctcagt gagtttatgg caggttataa tgcggtaacg   3780

gcagaatatc agcatgatac cagattgttt ccgcagggaa atttaccgga gaatcacctg   3840

aatatatcat cattaccgtg ggtgagtttt gacgggattt aacctgaaca tcaccggaaa   3900

tgatgattat tttgccccgg tttttacgat ggcaaagttt cagcaggaag gtgaccgcgt   3960

attattacct gtttctgtac aggttcatca tgcagtctgt gatggctttc atgcagcacg   4020

gtttattaat acacttcagc tgatgtgtga taacatactg aaataaatta attaattctg   4080

tatttaagcc accgtatccg gcaggaatgg tggctttttt tttatatttt aaccgtaatc   4140

tgtaatttcg tttcagactg gttcaggatg agctcgcttg gactcctgtt gatagatcca   4200

gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt   4260

tttattggtg agaatccaag cactagcggc gcgccggccg gcccggtgtg aaataccgca   4320

cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc   4380

gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg   4440

gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa   4500

ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga   4560

cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag   4620

ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct   4680

taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg   4740

ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc   4800

ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt   4860

aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta   4920

tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac   4980

agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc   5040

ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat   5100

tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc   5160

tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt   5220

cacctagatc cttttaaagg ccggccgcgg ccgccatcgg cattttcttt tgcgttttta   5280

tttgttaact gttaattgtc cttgttcaag gatgctgtct ttgacaacag atgttttctt   5340

gcctttgatg ttcagcagga agctcggcgc aaacgttgat tgtttgtctg cgtagaatcc   5400

tctgtttgtc atatagcttg taatcacgac attgtttcct ttcgcttgag gtacagcgaa   5460

gtgtgagtaa gtaaaggtta catcgttagg atcaagatcc atttttaaca caaggccagt   5520

tttgttcagc ggcttgtatg ggccagttaa agaattagaa acataaccaa gcatgtaaat   5580

atcgttagac gtaatgccgt caatcgtcat ttttgatccg cgggagtcag tgaacaggta   5640

ccatttgccg ttcattttaa agacgttcgc gcgttcaatt tcatctgtta ctgtgttaga   5700

tgcaatcagc ggtttcatca cttttttcag tgtgtaatca tcgtttagct caatcatacc   5760

gagagcgccg tttgctaact cagccgtgcg ttttttatcg ctttgcagaa gtttttgact   5820

ttcttgacgg aagaatgatg tgcttttgcc atagtatgct ttgttaaata aagattcttc   5880

gccttggtag ccatcttcag ttccagtgtt tgcttcaaat actaagtatt tgtggccttt   5940

atcttctacg tagtgaggat ctctcagcgt atggttgtcg cctgagctgt agttgccttc   6000

atcgatgaac tgctgtacat tttgatacgt ttttccgtca ccgtcaaaga ttgatttata   6060

atcctctaca ccgttgatgt tcaaagagct gtctgatgct gatacgttaa cttgtgcagt   6120

tgtcagtgtt tgtttgccgt aatgtttacc ggagaaatca gtgtagaata aacggatttt   6180

tccgtcagat gtaaatgtgg ctgaacctga ccattcttgt gtttggtctt ttaggataga   6240

atcatttgca tcgaatttgt cgctgtcttt aaagacgcgg ccagcgtttt tccagctgtc   6300

aatagaagtt tcgccgactt tttgatagaa catgtaaatc gatgtgtcat ccgcattttt   6360

aggatctccg gctaatgcaa agacgatgtg gtagccgtga tagtttgcga cagtgccgtc   6420

agcgttttgt aatggccagc tgtcccaaac gtccaggcct tttgcagaag agatattttt   6480

aattgtggac gaatcaaatt cagaaacttg atatttttca tttttttgct gttcagggat   6540

ttgcagcata tcatggcgtg taatatggga aatgccgtat gtttccttat atggcttttg   6600

gttcgtttct ttcgcaaacg cttgagttgc gcctcctgcc agcagtgcgg tagtaaaggt   6660

taatactgtt gcttgttttg caaacttttt gatgttcatc gttcatgtct ccttttttat   6720

gtactgtgtt agcggtctgc ttcttccagc cctcctgttt gaagatggca agttagttac   6780

gcacaataaa aaaagaccta aaatatgtaa ggggtgacgc caaagtatac actttgccct   6840

ttacacattt taggtcttgc ctgctttatc agtaacaaac ccgcgcgatt tacttttcga   6900

cctcattcta ttagactctc gtttggattg caactggtct attttcctct tttgtttgat   6960

agaaaatcat aaaaggattt gcagactacg ggcctaaaga actaaaaaat ctatctgttt   7020

cttttcattc tctgtatttt ttatagtttc tgttgcatgg gcataaagtt gcctttttaa   7080

tcacaattca gaaaatatca taatatctca tttcactaaa taatagtgaa cggcaggtat   7140

atgtgatggg ttaaaaagga tcggcggccg ctcgatttaa atc                     7183


<210>  8
<211>  7161
<212>  DNA
<213>  artificial sequence

<220>
<223>  complete nucleotide sequence of plasmid pSacB_delta_pflD

<400>  8
tcgagaggcc tgacgtcggg cccggtacca cgcgtcatat gactagttcg gacctaggga     60

tgggatcgag ctcttttcct tgccgacaag gcggaagctt taggggaaat tcccgtaggt    120

gccgtattgg tggatgaacg gggcaatatc attggtgaag gctggaacct ctctattgtg    180

aactcggatc ccaccgccca tgccgaaatt attgcgttgc gtaacgccgc gcagaaaatc    240

caaaattacc gcctgctcaa taccacttta tacgtgactt tagaaccctg caccatgtgc    300

gccggcgcga ttttacacag ccgaatcaaa cgcttggtat tcggggcgtc cgattacaaa    360

accggtgcgg tgggttccag atttcatttt tttgaggatt ataaaatgaa tcatggggtt    420

gagatcacaa gcggtgtctt ataggatcaa tgcagtcaga agttaagccg ctttttccaa    480

aagcgcaggg aacagaaaaa acaacaaaaa gctaccgcac ttttacaaca cccccggctt    540

aactcctctg aaaaatagtg acaaaaaaac cgtcataatg tttacgacgg tttttttatt    600

tcttctaata tgtcacatta agcccgtagc ctgcaagcaa ccccttaaca tgctccatta    660

attcttttgt cggcggtttt acatcttcaa gctcgtattt atcgccgagt acttcccatt    720

tatgggcgcc tagacggtga taaggtaata attccacttt ttcgatattc ttcatatctt    780

taatgaaatt ccccagcatg tgcaaatctt cgtcactatc tgtataaccc ggcactacaa    840

catggcggat ccaggtacgc tgatttcgat ccgctaaata ttttgcgaat tcgagcactc    900

ttttattcgg cacgccaatc aggctttcgt gaacccgttc attcatttct ttcaggtcaa    960

gcaacacaag atccgtgtca tcaatcaatt catcaataat atgatcatga tgacggacga   1020

aaccgttggt atccaagcaa gtattaattc cttctttatg gcaggctctg aaccagtccc   1080

gtacaaattc cgcctgtaaa atagcttcac cgccggaagc ggtaactccg ccgcccgagg   1140

cgttcataaa atggcgatag gtcaccactt ctttcattaa ttcttcaacg gaaatttctt   1200

taccgccgtg caaatcccag gtgtctctgt tatggcaata tttacaacgc attaagcagc   1260

cttgtaaaaa taaaataaag cggattcccg gcccgtcaac tgtcccgcag gtttcaaatg   1320

aatgaattcg tcctaaaacc gacataatat gcccttaaat aatcaacaaa atatagcaag   1380

aagattatag caaagaattt cgtttttttc agagaatagt caaatcttcg caaaaaacta   1440

ccgcactttt atccgcttta atcaggggaa ttaaaacaaa aaaattccgc ctattgaggc   1500

ggaatttatt aagcaataag acaaactctc aattttaata cttccttctt ttctagtatt   1560

gataagattg aaaccttgca aggatgacgg cggatttgcc gtcactctca cccaactaat   1620

gtggacgact ggtaaaccat tgcattagac caatgcaaac accaccaccg acgatgttac   1680

ctaaagtaac aggaattaaa tttttaatta ctaaatggta catatctaaa tttgcaaact   1740

gctcggcatt taaacccgtt gcctgccaga attccggcga tgcgaaattt gcaattacca   1800

tgcccatagg gatcataaac atatttgcta cgcagtgttc aaagcctgaa gcgacaaaya   1860

acccgatcgg caggatcata ataaaagctt tatccgttag agtyttgccg gcataggcca   1920

tccaaacggc aatacatacc ataatgttgc aaagaatacc taaacagaag gcttcaaycc   1980

aggtatgttc tattttatgt tgtgccgtat ttaaaatggt taatccccac tgaccgtttg   2040

ccgccatgat ctgaccggaa aaccaaatta atgcaacaat aaataaaccg ccgacaaaat   2100

taccgaarta aaccacaatc cagttacgta acatctgaat tgttgtaatt ttactctcaa   2160

agcgggcaat agtcgataaa gttgatgaag taaatagttc acagccgcaa accgccacca   2220

taattacccc gagagagaac accaaaccgc cgaccagttt agttaatccc caaggcgctc   2280

ccgcagaggc tgtttgagtt gttgtataaa aaacgaatgc aagagcaata aacataccgg   2340

cagagatcgc cgataaaaat gaataggctt gttttttcgt agctttataa acgccgacgt   2400

ctaacccggt ttgagccatc tcggttggcg aagccatcca agccaattta aaatcttccg   2460

atttcattga gctttcctta gtaataaaac tactcggaaa tgagtagaac tgccttaaag   2520

cataaatgat agattaaaaa atccaaaatt gttgaatatt atttaacggg gggattataa   2580

aagattcata aattagataa tagctaattt gagtgatcca tatcaccttt tacagatttt   2640

ttgacctaaa tcaaaattac ccaaatagag taataatacc attataaagg gtgtggattt   2700

attcctttgg tttacgagat aaattgctat ttaagctgat ttctgataaa aagtgcggta   2760

gatttttccc aaaaataagg aaacacaaaa tggcagaaga aacaattttc agtaaaatta   2820

ttcgtaaaga aattcccgcc gacattatat atcaagacga tcttgtcacc gcatttcgcg   2880

atattgcgcc gcaggcaaaa actcatattt taattattcc gaataaattg attccgacag   2940

taaacgacgt aaccgcccat cgtcgacatc gatgctcttc tgcgttaatt aacaattggg   3000

atcctctaga ctttgcttcc agatgtatgc tctcctccgg agagtaccgt gactttattt   3060

tcggcacaaa tacaggggtc gatggataaa tacggcgata gtttcctgac ggatgatccg   3120

tatgtaccgg cggaagacaa gctgcaaacc tgtcagatgg agattgattt aatggcggat   3180

gtgctgagag caccgccccg tgaatccgca gaactgatcc gctatgtgtt tgcggatgat   3240

tggccggaat aaataaagcc gggcttaata cagattaagc ccgtataggg tattattact   3300

gaataccaaa cagcttacgg aggacggaat gttacccatt gagacaacca gactgccttc   3360

tgattattaa tatttttcac tattaatcag aaggaataac catgaatttt acccggattg   3420

acctgaatac ctggaatcgc agggaacact ttgcccttta tcgtcagcag attaaatgcg   3480

gattcagcct gaccaccaaa ctcgatatta ccgctttgcg taccgcactg gcggagacag   3540

gttataagtt ttatccgctg atgatttacc tgatctcccg ggctgttaat cagtttccgg   3600

agttccggat ggcactgaaa gacaatgaac ttatttactg ggaccagtca gacccggtct   3660

ttactgtctt tcataaagaa accgaaacat tctctgcact gtcctgccgt tattttccgg   3720

atctcagtga gtttatggca ggttataatg cggtaacggc agaatatcag catgatacca   3780

gattgtttcc gcagggaaat ttaccggaga atcacctgaa tatatcatca ttaccgtggg   3840

tgagttttga cgggatttaa cctgaacatc accggaaatg atgattattt tgccccggtt   3900

tttacgatgg caaagtttca gcaggaaggt gaccgcgtat tattacctgt ttctgtacag   3960

gttcatcatg cagtctgtga tggctttcat gcagcacggt ttattaatac acttcagctg   4020

atgtgtgata acatactgaa ataaattaat taattctgta tttaagccac cgtatccggc   4080

aggaatggtg gctttttttt tatattttaa ccgtaatctg taatttcgtt tcagactggt   4140

tcaggatgag ctcgcttgga ctcctgttga tagatccagt aatgacctca gaactccatc   4200

tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatccaagca   4260

ctagcggcgc gccggccggc ccggtgtgaa ataccgcaca gatgcgtaag gagaaaatac   4320

cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg   4380

cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat   4440

aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc   4500

gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc   4560

tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga   4620

agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt   4680

ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg   4740

taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc   4800

gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg   4860

gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc   4920

ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg   4980

ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc   5040

gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct   5100

caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt   5160

taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaaggcc   5220

ggccgcggcc gccatcggca ttttcttttg cgtttttatt tgttaactgt taattgtcct   5280

tgttcaagga tgctgtcttt gacaacagat gttttcttgc ctttgatgtt cagcaggaag   5340

ctcggcgcaa acgttgattg tttgtctgcg tagaatcctc tgtttgtcat atagcttgta   5400

atcacgacat tgtttccttt cgcttgaggt acagcgaagt gtgagtaagt aaaggttaca   5460

tcgttaggat caagatccat ttttaacaca aggccagttt tgttcagcgg cttgtatggg   5520

ccagttaaag aattagaaac ataaccaagc atgtaaatat cgttagacgt aatgccgtca   5580

atcgtcattt ttgatccgcg ggagtcagtg aacaggtacc atttgccgtt cattttaaag   5640

acgttcgcgc gttcaatttc atctgttact gtgttagatg caatcagcgg tttcatcact   5700

tttttcagtg tgtaatcatc gtttagctca atcataccga gagcgccgtt tgctaactca   5760

gccgtgcgtt ttttatcgct ttgcagaagt ttttgacttt cttgacggaa gaatgatgtg   5820

cttttgccat agtatgcttt gttaaataaa gattcttcgc cttggtagcc atcttcagtt   5880

ccagtgtttg cttcaaatac taagtatttg tggcctttat cttctacgta gtgaggatct   5940

ctcagcgtat ggttgtcgcc tgagctgtag ttgccttcat cgatgaactg ctgtacattt   6000

tgatacgttt ttccgtcacc gtcaaagatt gatttataat cctctacacc gttgatgttc   6060

aaagagctgt ctgatgctga tacgttaact tgtgcagttg tcagtgtttg tttgccgtaa   6120

tgtttaccgg agaaatcagt gtagaataaa cggatttttc cgtcagatgt aaatgtggct   6180

gaacctgacc attcttgtgt ttggtctttt aggatagaat catttgcatc gaatttgtcg   6240

ctgtctttaa agacgcggcc agcgtttttc cagctgtcaa tagaagtttc gccgactttt   6300

tgatagaaca tgtaaatcga tgtgtcatcc gcatttttag gatctccggc taatgcaaag   6360

acgatgtggt agccgtgata gtttgcgaca gtgccgtcag cgttttgtaa tggccagctg   6420

tcccaaacgt ccaggccttt tgcagaagag atatttttaa ttgtggacga atcaaattca   6480

gaaacttgat atttttcatt tttttgctgt tcagggattt gcagcatatc atggcgtgta   6540

atatgggaaa tgccgtatgt ttccttatat ggcttttggt tcgtttcttt cgcaaacgct   6600

tgagttgcgc ctcctgccag cagtgcggta gtaaaggtta atactgttgc ttgttttgca   6660

aactttttga tgttcatcgt tcatgtctcc ttttttatgt actgtgttag cggtctgctt   6720

cttccagccc tcctgtttga agatggcaag ttagttacgc acaataaaaa aagacctaaa   6780

atatgtaagg ggtgacgcca aagtatacac tttgcccttt acacatttta ggtcttgcct   6840

gctttatcag taacaaaccc gcgcgattta cttttcgacc tcattctatt agactctcgt   6900

ttggattgca actggtctat tttcctcttt tgtttgatag aaaatcataa aaggatttgc   6960

agactacggg cctaaagaac taaaaaatct atctgtttct tttcattctc tgtatttttt   7020

atagtttctg ttgcatgggc ataaagttgc ctttttaatc acaattcaga aaatatcata   7080

atatctcatt tcactaaata atagtgaacg gcaggtatat gtgatgggtt aaaaaggatc   7140

ggcggccgct cgatttaaat c                                             7161


<210>  9
<211>  5363
<212>  DNA
<213>  artificial sequence

<220>
<223>  complete nucleotide sequence of plasmid pSacB_pykA1

<400>  9
tcgagcagaa gattaagaag aacgaaaatc gtatgtacaa tggggcctgc aacagacaaa     60

ggcaataatt tagaaaaaat cattgctgcc ggtgcaaacg ttgtacgtat gaacttctcc    120

cacggtacgc ccgaagatca tatcggtcgt gctgaaaaag tacgtgaaat cgctcataaa    180

ttaggtaaac acgtagcaat cttaggtgac ttacaaggcc ctaaaatccg tgtttctact    240

tttaaagaag gcaaaatttt cttaaatatc ggtgataaat tcattttaga cgcagagatg    300

cctaaaggtg aaggtaacca ggaagcggtt ggtttagact ataaaacatt accgcaagat    360

gtggttccgg gcgatatctt attattagat gacggtcgag ttcaattgaa agtattggca    420

accgaaggtg caaaagtatt caccgaagta acggtcggtg gcccactatc aaataataaa    480

ggcattaaca aattaggcgg cggtttatct gccgatgcat taaccgaaaa agataaagcg    540

gatatcatta ctgcggcgcg tatcggtgtg gattaccttg ccgtatcttt cccgcgttca    600

agcgcggatt taaactacgc ccgtcaatta gcaaaagatg cgggcttgga tgcgaaaatc    660

gttgcgaaag tagaacgtgc cgaaacagtt gaaacggacg aagcaatgga cgatatcatc    720

aatgcggcgg acgtaatcat ggttgcgcgc ggtgacttag gtgttgaaat cggtgatccg    780

gaattagtcg gtgttcagaa aaaattaatc cgtcgttcac gtcagttaaa tcgtgttgtt    840

attaccgcaa ctcaaatgat ggaatcaatg attagtaatc ctatgccgac tcgtgcggaa    900

gtaatggacg tagctaacgc agtattggac ggtaccgatg cggtaatgct ttctgctgaa    960

accgcggctg gtcaatatcc ggcggaaact gttgcggcga tggcgaaagt tgcgttaggt   1020

gcggagaaaa tgccaagcat taatgtgtct aaacaccgta tgaacgttca attcgagtct   1080

attgaagaat ctgttgcgat gtctgcaatg tatgcggcaa accacatgag aggcgtagcg   1140

gcgattatca cattaacaag tagcggtcgt actgctcgtt taatgtctag actccatagg   1200

ccgctttcct ggctttgctt ccagatgtat gctctcctcc ggagagtacc gtgactttat   1260

tttcggcaca aatacagggg tcgatggata aatacggcga tagtttcctg acggatgatc   1320

cgtatgtacc ggcggaagac aagctgcaaa cctgtcagat ggagattgat ttaatggcgg   1380

atgtgctgag agcaccgccc cgtgaatccg cagaactgat ccgctatgtg tttgcggatg   1440

attggccgga ataaataaag ccgggcttaa tacagattaa gcccgtatag ggtattatta   1500

ctgaatacca aacagcttac ggaggacgga atgttaccca ttgagacaac cagactgcct   1560

tctgattatt aatatttttc actattaatc agaaggaata accatgaatt ttacccggat   1620

tgacctgaat acctggaatc gcagggaaca ctttgccctt tatcgtcagc agattaaatg   1680

cggattcagc ctgaccacca aactcgatat taccgctttg cgtaccgcac tggcggagac   1740

aggttataag ttttatccgc tgatgattta cctgatctcc cgggctgtta atcagtttcc   1800

ggagttccgg atggcactga aagacaatga acttatttac tgggaccagt cagacccggt   1860

ctttactgtc tttcataaag aaaccgaaac attctctgca ctgtcctgcc gttattttcc   1920

ggatctcagt gagtttatgg caggttataa tgcggtaacg gcagaatatc agcatgatac   1980

cagattgttt ccgcagggaa atttaccgga gaatcacctg aatatatcat cattaccgtg   2040

ggtgagtttt gacgggattt aacctgaaca tcaccggaaa tgatgattat tttgccccgg   2100

tttttacgat ggcaaagttt cagcaggaag gtgaccgcgt attattacct gtttctgtac   2160

aggttcatca tgcagtctgt gatggctttc atgcagcacg gtttattaat acacttcagc   2220

tgatgtgtga taacatactg aaataaatta attaattctg tatttaagcc accgtatccg   2280

gcaggaatgg tggctttttt tttatatttt aaccgtaatc tgtaatttcg tttcagactg   2340

gttcaggatg agctcgcttg gactcctgtt gatagatcca gtaatgacct cagaactcca   2400

tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccaag   2460

cactagcggc gcgccggccg gcccggtgtg aaataccgca cagatgcgta aggagaaaat   2520

accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc   2580

tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg   2640

ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg   2700

ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac   2760

gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg   2820

gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct   2880

ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg   2940

tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct   3000

gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac   3060

tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt   3120

tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc   3180

tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca   3240

ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat   3300

ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac   3360

gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaagg   3420

ccggccgcgg ccgccatcgg cattttcttt tgcgttttta tttgttaact gttaattgtc   3480

cttgttcaag gatgctgtct ttgacaacag atgttttctt gcctttgatg ttcagcagga   3540

agctcggcgc aaacgttgat tgtttgtctg cgtagaatcc tctgtttgtc atatagcttg   3600

taatcacgac attgtttcct ttcgcttgag gtacagcgaa gtgtgagtaa gtaaaggtta   3660

catcgttagg atcaagatcc atttttaaca caaggccagt tttgttcagc ggcttgtatg   3720

ggccagttaa agaattagaa acataaccaa gcatgtaaat atcgttagac gtaatgccgt   3780

caatcgtcat ttttgatccg cgggagtcag tgaacaggta ccatttgccg ttcattttaa   3840

agacgttcgc gcgttcaatt tcatctgtta ctgtgttaga tgcaatcagc ggtttcatca   3900

cttttttcag tgtgtaatca tcgtttagct caatcatacc gagagcgccg tttgctaact   3960

cagccgtgcg ttttttatcg ctttgcagaa gtttttgact ttcttgacgg aagaatgatg   4020

tgcttttgcc atagtatgct ttgttaaata aagattcttc gccttggtag ccatcttcag   4080

ttccagtgtt tgcttcaaat actaagtatt tgtggccttt atcttctacg tagtgaggat   4140

ctctcagcgt atggttgtcg cctgagctgt agttgccttc atcgatgaac tgctgtacat   4200

tttgatacgt ttttccgtca ccgtcaaaga ttgatttata atcctctaca ccgttgatgt   4260

tcaaagagct gtctgatgct gatacgttaa cttgtgcagt tgtcagtgtt tgtttgccgt   4320

aatgtttacc ggagaaatca gtgtagaata aacggatttt tccgtcagat gtaaatgtgg   4380

ctgaacctga ccattcttgt gtttggtctt ttaggataga atcatttgca tcgaatttgt   4440

cgctgtcttt aaagacgcgg ccagcgtttt tccagctgtc aatagaagtt tcgccgactt   4500

tttgatagaa catgtaaatc gatgtgtcat ccgcattttt aggatctccg gctaatgcaa   4560

agacgatgtg gtagccgtga tagtttgcga cagtgccgtc agcgttttgt aatggccagc   4620

tgtcccaaac gtccaggcct tttgcagaag agatattttt aattgtggac gaatcaaatt   4680

cagaaacttg atatttttca tttttttgct gttcagggat ttgcagcata tcatggcgtg   4740

taatatggga aatgccgtat gtttccttat atggcttttg gttcgtttct ttcgcaaacg   4800

cttgagttgc gcctcctgcc agcagtgcgg tagtaaaggt taatactgtt gcttgttttg   4860

caaacttttt gatgttcatc gttcatgtct ccttttttat gtactgtgtt agcggtctgc   4920

ttcttccagc cctcctgttt gaagatggca agttagttac gcacaataaa aaaagaccta   4980

aaatatgtaa ggggtgacgc caaagtatac actttgccct ttacacattt taggtcttgc   5040

ctgctttatc agtaacaaac ccgcgcgatt tacttttcga cctcattcta ttagactctc   5100

gtttggattg caactggtct attttcctct tttgtttgat agaaaatcat aaaaggattt   5160

gcagactacg ggcctaaaga actaaaaaat ctatctgttt cttttcattc tctgtatttt   5220

ttatagtttc tgttgcatgg gcataaagtt gcctttttaa tcacaattca gaaaatatca   5280

taatatctca tttcactaaa taatagtgaa cggcaggtat atgtgatggg ttaaaaagga   5340

tcggcggccg ctcgatttaa atc                                           5363


<210>  10
<211>  5363
<212>  DNA
<213>  artificial sequence

<220>
<223>  complete nucleotide sequence of plasmid pSacB_pykA2

<400>  10
tcgagcagaa gattaagaag aacgaaaatc gtatgtacaa tggggcctgc aacagacaaa     60

ggcaataatt tagaaaaaat cattgctgcc ggtgcaaacg ttgtacgtat gaacttctcc    120

cacggtacgc ccgaagatca tatcggtcgt gctgaaaaag tacgtgaaat cgctcataaa    180

ttaggtaaac acgtagcaat cttaggtgac ttacaaggcc ctaaaatccg tgtttctact    240

tttaaagaag gcaaaatttt cttaaatatc ggtgataaat tcattttaga cgcagagatg    300

cctaaaggtg aaggtaacca ggaagcggtt ggtttagact ataaaacatt accgcaagat    360

gtggttccgg gcgatatctt attattagat gacggtcgag ttcaattgaa agtattggca    420

accgaaggtg caaaagtatt caccgaagta acggtcggtg gcccactatc aaataataaa    480

ggcattaaca aattaggctg cggtttatct gccgatgcat taaccgaaaa agataaagcg    540

gatatcatta ctgcggcgcg tatcggtgtg gattaccttg ccgtatcttt cccgcgttca    600

agcgcggatt taaactacgc ccgtcaatta gcaaaagatg cgggcttgga tgcgaaaatc    660

gttgcgaaag tagaacgtgc cgaaacagtt gaaacggacg aagcaatgga cgatatcatc    720

aatgcggcgg acgtaatcat ggttgcgcgc ggtgacttag gtgttgaaat cggtgatccg    780

gaattagtcg gtgttcagaa aaaattaatc cgtcgttcac gtcagttaaa tcgtgttgtt    840

attaccgcaa ctcaaatgat ggaatcaatg attagtaatc ctatgccgac tcgtgcggaa    900

gtaatggacg tagctaacgc agtattggac ggtaccgatg cggtaatgct ttctgctgaa    960

accgcggctg gtcaatatcc ggcggaaact gttgcggcga tggcgaaagt tgcgttaggt   1020

gcggagaaaa tgccaagcat taatgtgtct aaacaccgta tgaacgttca attcgagtct   1080

attgaagaat ctgttgcgat gtctgcaatg tatgcggcaa accacatgag aggcgtagcg   1140

gcgattatca cattaacaag tagcggtcgt actgctcgtt taatgtctag actccatagg   1200

ccgctttcct ggctttgctt ccagatgtat gctctcctcc ggagagtacc gtgactttat   1260

tttcggcaca aatacagggg tcgatggata aatacggcga tagtttcctg acggatgatc   1320

cgtatgtacc ggcggaagac aagctgcaaa cctgtcagat ggagattgat ttaatggcgg   1380

atgtgctgag agcaccgccc cgtgaatccg cagaactgat ccgctatgtg tttgcggatg   1440

attggccgga ataaataaag ccgggcttaa tacagattaa gcccgtatag ggtattatta   1500

ctgaatacca aacagcttac ggaggacgga atgttaccca ttgagacaac cagactgcct   1560

tctgattatt aatatttttc actattaatc agaaggaata accatgaatt ttacccggat   1620

tgacctgaat acctggaatc gcagggaaca ctttgccctt tatcgtcagc agattaaatg   1680

cggattcagc ctgaccacca aactcgatat taccgctttg cgtaccgcac tggcggagac   1740

aggttataag ttttatccgc tgatgattta cctgatctcc cgggctgtta atcagtttcc   1800

ggagttccgg atggcactga aagacaatga acttatttac tgggaccagt cagacccggt   1860

ctttactgtc tttcataaag aaaccgaaac attctctgca ctgtcctgcc gttattttcc   1920

ggatctcagt gagtttatgg caggttataa tgcggtaacg gcagaatatc agcatgatac   1980

cagattgttt ccgcagggaa atttaccgga gaatcacctg aatatatcat cattaccgtg   2040

ggtgagtttt gacgggattt aacctgaaca tcaccggaaa tgatgattat tttgccccgg   2100

tttttacgat ggcaaagttt cagcaggaag gtgaccgcgt attattacct gtttctgtac   2160

aggttcatca tgcagtctgt gatggctttc atgcagcacg gtttattaat acacttcagc   2220

tgatgtgtga taacatactg aaataaatta attaattctg tatttaagcc accgtatccg   2280

gcaggaatgg tggctttttt tttatatttt aaccgtaatc tgtaatttcg tttcagactg   2340

gttcaggatg agctcgcttg gactcctgtt gatagatcca gtaatgacct cagaactcca   2400

tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccaag   2460

cactagcggc gcgccggccg gcccggtgtg aaataccgca cagatgcgta aggagaaaat   2520

accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc   2580

tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg   2640

ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg   2700

ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac   2760

gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg   2820

gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct   2880

ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg   2940

tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct   3000

gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac   3060

tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt   3120

tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc   3180

tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca   3240

ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat   3300

ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac   3360

gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaagg   3420

ccggccgcgg ccgccatcgg cattttcttt tgcgttttta tttgttaact gttaattgtc   3480

cttgttcaag gatgctgtct ttgacaacag atgttttctt gcctttgatg ttcagcagga   3540

agctcggcgc aaacgttgat tgtttgtctg cgtagaatcc tctgtttgtc atatagcttg   3600

taatcacgac attgtttcct ttcgcttgag gtacagcgaa gtgtgagtaa gtaaaggtta   3660

catcgttagg atcaagatcc atttttaaca caaggccagt tttgttcagc ggcttgtatg   3720

ggccagttaa agaattagaa acataaccaa gcatgtaaat atcgttagac gtaatgccgt   3780

caatcgtcat ttttgatccg cgggagtcag tgaacaggta ccatttgccg ttcattttaa   3840

agacgttcgc gcgttcaatt tcatctgtta ctgtgttaga tgcaatcagc ggtttcatca   3900

cttttttcag tgtgtaatca tcgtttagct caatcatacc gagagcgccg tttgctaact   3960

cagccgtgcg ttttttatcg ctttgcagaa gtttttgact ttcttgacgg aagaatgatg   4020

tgcttttgcc atagtatgct ttgttaaata aagattcttc gccttggtag ccatcttcag   4080

ttccagtgtt tgcttcaaat actaagtatt tgtggccttt atcttctacg tagtgaggat   4140

ctctcagcgt atggttgtcg cctgagctgt agttgccttc atcgatgaac tgctgtacat   4200

tttgatacgt ttttccgtca ccgtcaaaga ttgatttata atcctctaca ccgttgatgt   4260

tcaaagagct gtctgatgct gatacgttaa cttgtgcagt tgtcagtgtt tgtttgccgt   4320

aatgtttacc ggagaaatca gtgtagaata aacggatttt tccgtcagat gtaaatgtgg   4380

ctgaacctga ccattcttgt gtttggtctt ttaggataga atcatttgca tcgaatttgt   4440

cgctgtcttt aaagacgcgg ccagcgtttt tccagctgtc aatagaagtt tcgccgactt   4500

tttgatagaa catgtaaatc gatgtgtcat ccgcattttt aggatctccg gctaatgcaa   4560

agacgatgtg gtagccgtga tagtttgcga cagtgccgtc agcgttttgt aatggccagc   4620

tgtcccaaac gtccaggcct tttgcagaag agatattttt aattgtggac gaatcaaatt   4680

cagaaacttg atatttttca tttttttgct gttcagggat ttgcagcata tcatggcgtg   4740

taatatggga aatgccgtat gtttccttat atggcttttg gttcgtttct ttcgcaaacg   4800

cttgagttgc gcctcctgcc agcagtgcgg tagtaaaggt taatactgtt gcttgttttg   4860

caaacttttt gatgttcatc gttcatgtct ccttttttat gtactgtgtt agcggtctgc   4920

ttcttccagc cctcctgttt gaagatggca agttagttac gcacaataaa aaaagaccta   4980

aaatatgtaa ggggtgacgc caaagtatac actttgccct ttacacattt taggtcttgc   5040

ctgctttatc agtaacaaac ccgcgcgatt tacttttcga cctcattcta ttagactctc   5100

gtttggattg caactggtct attttcctct tttgtttgat agaaaatcat aaaaggattt   5160

gcagactacg ggcctaaaga actaaaaaat ctatctgttt cttttcattc tctgtatttt   5220

ttatagtttc tgttgcatgg gcataaagtt gcctttttaa tcacaattca gaaaatatca   5280

taatatctca tttcactaaa taatagtgaa cggcaggtat atgtgatggg ttaaaaagga   5340

tcggcggccg ctcgatttaa atc                                           5363


<210>  11
<211>  5634
<212>  DNA
<213>  artificial sequence

<220>
<223>  complete nucleotide sequence of plasmid pSacB_pykA3

<400>  11
tcgagagcgg atatcattac tgcggcgcgt atcggtgtgg attaccttgc cgtatctttc     60

ccgcgttcaa gcgcggattt aaactacgcc cgtcaattag caaaagatgc gggcttggat    120

gcgaaaatcg ttgcgaaagt agaacgtgcc gaaacagttg aaacggacga agcaatggac    180

gatatcatca atgcggcgga cgtaatcatg gttgcgcgcg gtgacttagg tgttgaaatc    240

ggtgatccgg aattagtcgg tgttcagaaa aaattaatcc gtcgttcacg tcagttaaat    300

cgtgttgtta ttaccgcaac tcaaatgatg gaatcaatga ttagtaatcc tatgccgact    360

cgtgcggaag taatggacgt agctaacgca gtattggacg gtaccgatgc ggtaatgctt    420

tctgctgaaa ccgcggctgg tcaatatccg gcggaaactg ttgcggcgat ggcgaaagtt    480

gcgttaggtg cggagaaaat gccaagcatt aatgtgtcta aacaccgtat gaacgttcaa    540

ttcgagtcta ttgaagaatc tgttgcgatg tctgcaatgt atgcggcaaa ccacatgaga    600

ggcgtagcgg cgattatcac attaacaagt agcggtcgta ctgctcgttt aatgtctcgc    660

attagttccg gtttaccaat ctttgcattg tcacgtaacg aatctacatt aaacttatgc    720

gcattatatc gtggtgtgac accggttcat tttgataaag acagccgtac ctcagaaggt    780

gcgacagcgg cggttcaatt attaaaagac gaaggtttct tagtgtctgg cgatttagtg    840

ttattaactc agggcgacgc aagcagttct agcggtacta acctttgccg tacattgatt    900

gttgaataat aggcaatgaa caaaaaaacg atgatttaag tcatcgtttt ttttttttgc    960

ttttctataa aaattcggaa aaatgcaccg cactatttgt ttaacagatc ttttaagccc   1020

gccttcattg ccgacatctg gctttcatac aacgctttgc tttcccgctc gtcgatcata   1080

taggtgatgg tttcggaaag tgtcattttc atctttttcg aatatttgga aaggcgtaac   1140

caaaccccat attctaaatc aatagatttt ttcttcgtgg ataatttttc cgcgttaaag   1200

aaacgtttac gtctggctcg aatcgcctga tctaatttga taatcaacga ttcagccata   1260

tgattggcta tccattcttc aattttttca ggataattct ggctttctaa taaatcatgc   1320

actttgcttt gctgcaaact gcgttcttca taacgggtaa tattctcgcc ttcacgattt   1380

tttttaatta aatacaacca tttccaatgc gcttcttgat tttctaattt ttgatacttc   1440

atggataggt tctccatcta gactccatag gccgctttcc tggctttgct tccagatgta   1500

tgctctcctc cggagagtac cgtgacttta ttttcggcac aaatacaggg gtcgatggat   1560

aaatacggcg atagtttcct gacggatgat ccgtatgtac cggcggaaga caagctgcaa   1620

acctgtcaga tggagattga tttaatggcg gatgtgctga gagcaccgcc ccgtgaatcc   1680

gcagaactga tccgctatgt gtttgcggat gattggccgg aataaataaa gccgggctta   1740

atacagatta agcccgtata gggtattatt actgaatacc aaacagctta cggaggacgg   1800

aatgttaccc attgagacaa ccagactgcc ttctgattat taatattttt cactattaat   1860

cagaaggaat aaccatgaat tttacccgga ttgacctgaa tacctggaat cgcagggaac   1920

actttgccct ttatcgtcag cagattaaat gcggattcag cctgaccacc aaactcgata   1980

ttaccgcttt gcgtaccgca ctggcggaga caggttataa gttttatccg ctgatgattt   2040

acctgatctc ccgggctgtt aatcagtttc cggagttccg gatggcactg aaagacaatg   2100

aacttattta ctgggaccag tcagacccgg tctttactgt ctttcataaa gaaaccgaaa   2160

cattctctgc actgtcctgc cgttattttc cggatctcag tgagtttatg gcaggttata   2220

atgcggtaac ggcagaatat cagcatgata ccagattgtt tccgcaggga aatttaccgg   2280

agaatcacct gaatatatca tcattaccgt gggtgagttt tgacgggatt taacctgaac   2340

atcaccggaa atgatgatta ttttgccccg gtttttacga tggcaaagtt tcagcaggaa   2400

ggtgaccgcg tattattacc tgtttctgta caggttcatc atgcagtctg tgatggcttt   2460

catgcagcac ggtttattaa tacacttcag ctgatgtgtg ataacatact gaaataaatt   2520

aattaattct gtatttaagc caccgtatcc ggcaggaatg gtggcttttt ttttatattt   2580

taaccgtaat ctgtaatttc gtttcagact ggttcaggat gagctcgctt ggactcctgt   2640

tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc   2700

gccgggcgtt ttttattggt gagaatccaa gcactagcgg cgcgccggcc ggcccggtgt   2760

gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg   2820

ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag   2880

gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa   2940

ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc   3000

cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca   3060

ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg   3120

accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct   3180

catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt   3240

gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag   3300

tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc   3360

agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac   3420

actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga   3480

gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc   3540

aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg   3600

gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca   3660

aaaaggatct tcacctagat ccttttaaag gccggccgcg gccgccatcg gcattttctt   3720

ttgcgttttt atttgttaac tgttaattgt ccttgttcaa ggatgctgtc tttgacaaca   3780

gatgttttct tgcctttgat gttcagcagg aagctcggcg caaacgttga ttgtttgtct   3840

gcgtagaatc ctctgtttgt catatagctt gtaatcacga cattgtttcc tttcgcttga   3900

ggtacagcga agtgtgagta agtaaaggtt acatcgttag gatcaagatc catttttaac   3960

acaaggccag ttttgttcag cggcttgtat gggccagtta aagaattaga aacataacca   4020

agcatgtaaa tatcgttaga cgtaatgccg tcaatcgtca tttttgatcc gcgggagtca   4080

gtgaacaggt accatttgcc gttcatttta aagacgttcg cgcgttcaat ttcatctgtt   4140

actgtgttag atgcaatcag cggtttcatc acttttttca gtgtgtaatc atcgtttagc   4200

tcaatcatac cgagagcgcc gtttgctaac tcagccgtgc gttttttatc gctttgcaga   4260

agtttttgac tttcttgacg gaagaatgat gtgcttttgc catagtatgc tttgttaaat   4320

aaagattctt cgccttggta gccatcttca gttccagtgt ttgcttcaaa tactaagtat   4380

ttgtggcctt tatcttctac gtagtgagga tctctcagcg tatggttgtc gcctgagctg   4440

tagttgcctt catcgatgaa ctgctgtaca ttttgatacg tttttccgtc accgtcaaag   4500

attgatttat aatcctctac accgttgatg ttcaaagagc tgtctgatgc tgatacgtta   4560

acttgtgcag ttgtcagtgt ttgtttgccg taatgtttac cggagaaatc agtgtagaat   4620

aaacggattt ttccgtcaga tgtaaatgtg gctgaacctg accattcttg tgtttggtct   4680

tttaggatag aatcatttgc atcgaatttg tcgctgtctt taaagacgcg gccagcgttt   4740

ttccagctgt caatagaagt ttcgccgact ttttgataga acatgtaaat cgatgtgtca   4800

tccgcatttt taggatctcc ggctaatgca aagacgatgt ggtagccgtg atagtttgcg   4860

acagtgccgt cagcgttttg taatggccag ctgtcccaaa cgtccaggcc ttttgcagaa   4920

gagatatttt taattgtgga cgaatcaaat tcagaaactt gatatttttc atttttttgc   4980

tgttcaggga tttgcagcat atcatggcgt gtaatatggg aaatgccgta tgtttcctta   5040

tatggctttt ggttcgtttc tttcgcaaac gcttgagttg cgcctcctgc cagcagtgcg   5100

gtagtaaagg ttaatactgt tgcttgtttt gcaaactttt tgatgttcat cgttcatgtc   5160

tcctttttta tgtactgtgt tagcggtctg cttcttccag ccctcctgtt tgaagatggc   5220

aagttagtta cgcacaataa aaaaagacct aaaatatgta aggggtgacg ccaaagtata   5280

cactttgccc tttacacatt ttaggtcttg cctgctttat cagtaacaaa cccgcgcgat   5340

ttacttttcg acctcattct attagactct cgtttggatt gcaactggtc tattttcctc   5400

ttttgtttga tagaaaatca taaaaggatt tgcagactac gggcctaaag aactaaaaaa   5460

tctatctgtt tcttttcatt ctctgtattt tttatagttt ctgttgcatg ggcataaagt   5520

tgccttttta atcacaattc agaaaatatc ataatatctc atttcactaa ataatagtga   5580

acggcaggta tatgtgatgg gttaaaaagg atcggcggcc gctcgattta aatc         5634


<210>  12
<211>  5634
<212>  DNA
<213>  artificial sequence

<220>
<223>  complete nucleotide sequence of plasmid pSacB_pykA4

<400>  12
tcgagagcgg atatcattac tgcggcgcgt atcggtgtgg attaccttgc cgtatctttc     60

ccgcgttcaa gcgcggattt aaactacgcc cgtcaattag caaaagatgc gggcttggat    120

gcgaaaatcg ttgcgaaagt agaacgtgcc gaaacagttg aaacggacga agcaatggac    180

gatatcatca atgcggcgga cgtaatcatg gttgcgcgcg gtgacttagg tgttgaaatc    240

ggtgatccgg aattagtcgg tgttcagaaa aaattaatcc gtcgttcacg tcagttaaat    300

cgtgttgtta ttaccgcaac tcaaatgatg gaatcaatga ttagtaatcc tatgccgact    360

cgtgcggaag taatggacgt agctaacgca gtattggacg gtaccgatgc ggtaatgctt    420

tctgctgaaa ccgcggctgg tcaatatccg gcggaaactg ttgcggcgat ggcgaaagtt    480

gcgttaggtg cggagaaaat gccaagcatt aatgtgtcta aacaccgtat gaacgttcaa    540

ttcgagtcta ttgaagaatc tgttgcgatg tctgcaatgt atgcggcaaa ccacatgaga    600

ggcgtagcgg cgattatcac attaacaagt agcggtcgta ctgctcgttt aatgtctcgc    660

attagttccg gtttaccaat ctttgcattg tcacgtaacg aatctacttt aaacttatac    720

gcattatatc gtggtgtgac accggttcat tttgataaag acagccgtac ctcagaaggt    780

gcgacagcgg cggttcaatt attaaaagac gaaggtttct tagtgtctgg cgatttagtg    840

ttattaactc agggcgacgc aagcagttct agcggtacta acctttgccg tacattgatt    900

gttgaataat aggcaatgaa caaaaaaacg atgatttaag tcatcgtttt ttttttttgc    960

ttttctataa aaattcggaa aaatgcaccg cactatttgt ttaacagatc ttttaagccc   1020

gccttcattg ccgacatctg gctttcatac aacgctttgc tttcccgctc gtcgatcata   1080

taggtgatgg tttcggaaag tgtcattttc atctttttcg aatatttgga aaggcgtaac   1140

caaaccccat attctaaatc aatagatttt ttcttcgtgg ataatttttc cgcgttaaag   1200

aaacgtttac gtctggctcg aatcgcctga tctaatttga taatcaacga ttcagccata   1260

tgattggcta tccattcttc aattttttca ggataattct ggctttctaa taaatcatgc   1320

actttgcttt gctgcaaact gcgttcttca taacgggtaa tattctcgcc ttcacgattt   1380

tttttaatta aatacaacca tttccaatgc gcttcttgat tttctaattt ttgatacttc   1440

atggataggt tctccatcta gactccatag gccgctttcc tggctttgct tccagatgta   1500

tgctctcctc cggagagtac cgtgacttta ttttcggcac aaatacaggg gtcgatggat   1560

aaatacggcg atagtttcct gacggatgat ccgtatgtac cggcggaaga caagctgcaa   1620

acctgtcaga tggagattga tttaatggcg gatgtgctga gagcaccgcc ccgtgaatcc   1680

gcagaactga tccgctatgt gtttgcggat gattggccgg aataaataaa gccgggctta   1740

atacagatta agcccgtata gggtattatt actgaatacc aaacagctta cggaggacgg   1800

aatgttaccc attgagacaa ccagactgcc ttctgattat taatattttt cactattaat   1860

cagaaggaat aaccatgaat tttacccgga ttgacctgaa tacctggaat cgcagggaac   1920

actttgccct ttatcgtcag cagattaaat gcggattcag cctgaccacc aaactcgata   1980

ttaccgcttt gcgtaccgca ctggcggaga caggttataa gttttatccg ctgatgattt   2040

acctgatctc ccgggctgtt aatcagtttc cggagttccg gatggcactg aaagacaatg   2100

aacttattta ctgggaccag tcagacccgg tctttactgt ctttcataaa gaaaccgaaa   2160

cattctctgc actgtcctgc cgttattttc cggatctcag tgagtttatg gcaggttata   2220

atgcggtaac ggcagaatat cagcatgata ccagattgtt tccgcaggga aatttaccgg   2280

agaatcacct gaatatatca tcattaccgt gggtgagttt tgacgggatt taacctgaac   2340

atcaccggaa atgatgatta ttttgccccg gtttttacga tggcaaagtt tcagcaggaa   2400

ggtgaccgcg tattattacc tgtttctgta caggttcatc atgcagtctg tgatggcttt   2460

catgcagcac ggtttattaa tacacttcag ctgatgtgtg ataacatact gaaataaatt   2520

aattaattct gtatttaagc caccgtatcc ggcaggaatg gtggcttttt ttttatattt   2580

taaccgtaat ctgtaatttc gtttcagact ggttcaggat gagctcgctt ggactcctgt   2640

tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc   2700

gccgggcgtt ttttattggt gagaatccaa gcactagcgg cgcgccggcc ggcccggtgt   2760

gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg   2820

ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag   2880

gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa   2940

ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc   3000

cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca   3060

ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg   3120

accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct   3180

catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt   3240

gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag   3300

tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc   3360

agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac   3420

actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga   3480

gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc   3540

aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg   3600

gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca   3660

aaaaggatct tcacctagat ccttttaaag gccggccgcg gccgccatcg gcattttctt   3720

ttgcgttttt atttgttaac tgttaattgt ccttgttcaa ggatgctgtc tttgacaaca   3780

gatgttttct tgcctttgat gttcagcagg aagctcggcg caaacgttga ttgtttgtct   3840

gcgtagaatc ctctgtttgt catatagctt gtaatcacga cattgtttcc tttcgcttga   3900

ggtacagcga agtgtgagta agtaaaggtt acatcgttag gatcaagatc catttttaac   3960

acaaggccag ttttgttcag cggcttgtat gggccagtta aagaattaga aacataacca   4020

agcatgtaaa tatcgttaga cgtaatgccg tcaatcgtca tttttgatcc gcgggagtca   4080

gtgaacaggt accatttgcc gttcatttta aagacgttcg cgcgttcaat ttcatctgtt   4140

actgtgttag atgcaatcag cggtttcatc acttttttca gtgtgtaatc atcgtttagc   4200

tcaatcatac cgagagcgcc gtttgctaac tcagccgtgc gttttttatc gctttgcaga   4260

agtttttgac tttcttgacg gaagaatgat gtgcttttgc catagtatgc tttgttaaat   4320

aaagattctt cgccttggta gccatcttca gttccagtgt ttgcttcaaa tactaagtat   4380

ttgtggcctt tatcttctac gtagtgagga tctctcagcg tatggttgtc gcctgagctg   4440

tagttgcctt catcgatgaa ctgctgtaca ttttgatacg tttttccgtc accgtcaaag   4500

attgatttat aatcctctac accgttgatg ttcaaagagc tgtctgatgc tgatacgtta   4560

acttgtgcag ttgtcagtgt ttgtttgccg taatgtttac cggagaaatc agtgtagaat   4620

aaacggattt ttccgtcaga tgtaaatgtg gctgaacctg accattcttg tgtttggtct   4680

tttaggatag aatcatttgc atcgaatttg tcgctgtctt taaagacgcg gccagcgttt   4740

ttccagctgt caatagaagt ttcgccgact ttttgataga acatgtaaat cgatgtgtca   4800

tccgcatttt taggatctcc ggctaatgca aagacgatgt ggtagccgtg atagtttgcg   4860

acagtgccgt cagcgttttg taatggccag ctgtcccaaa cgtccaggcc ttttgcagaa   4920

gagatatttt taattgtgga cgaatcaaat tcagaaactt gatatttttc atttttttgc   4980

tgttcaggga tttgcagcat atcatggcgt gtaatatggg aaatgccgta tgtttcctta   5040

tatggctttt ggttcgtttc tttcgcaaac gcttgagttg cgcctcctgc cagcagtgcg   5100

gtagtaaagg ttaatactgt tgcttgtttt gcaaactttt tgatgttcat cgttcatgtc   5160

tcctttttta tgtactgtgt tagcggtctg cttcttccag ccctcctgtt tgaagatggc   5220

aagttagtta cgcacaataa aaaaagacct aaaatatgta aggggtgacg ccaaagtata   5280

cactttgccc tttacacatt ttaggtcttg cctgctttat cagtaacaaa cccgcgcgat   5340

ttacttttcg acctcattct attagactct cgtttggatt gcaactggtc tattttcctc   5400

ttttgtttga tagaaaatca taaaaggatt tgcagactac gggcctaaag aactaaaaaa   5460

tctatctgtt tcttttcatt ctctgtattt tttatagttt ctgttgcatg ggcataaagt   5520

tgccttttta atcacaattc agaaaatatc ataatatctc atttcactaa ataatagtga   5580

acggcaggta tatgtgatgg gttaaaaagg atcggcggcc gctcgattta aatc         5634


<210>  13
<211>  5363
<212>  DNA
<213>  artificial sequence

<220>
<223>  complete nucleotid sequence of plasmid pSacB_pykA5

<400>  13
tcgagcagaa gattaagaag aacgaaaatc gtatgtacaa tggggcctgc aacagacaaa     60

ggcaataatt tagaaaaaat cattgctgcc ggtgcaaacg ttgtacgtat gaacttctcc    120

cacggtacgc ccgaagatca tatcggtcgt gctgaaaaag tacgtgaaat cgctcataaa    180

ttaggtaaac acgtagcaat cttaggtgac ttacaaggcc ctaaaatccg tgtttctact    240

tttaaagaag gcaaaatttt cttaaatatc ggtgataaat tcattttaga cgcagagatg    300

cctaaaggtg aaggtaacca ggaagcggtt ggtttagact ataaaacatt accgcaagat    360

gtggttccgg gcgatatctt attattagat gacggtcgag ttcaattgaa agtattggca    420

accgaaggtg caaaagtatt caccgaagta acggtcggtg gcccactatc aaataataaa    480

ggcattaaca aattaggcgg cggtttatct ggcgatgcgt taaccgaaaa agataaagcg    540

gatatcatta ctgcggcgcg tatcggtgtg gattaccttg ccgtatcttt cccgcgttca    600

agcgcggatt taaactacgc ccgtcaatta gcaaaagatg cgggcttgga tgcgaaaatc    660

gttgcgaaag tagaacgtgc cgaaacagtt gaaacggacg aagcaatgga cgatatcatc    720

aatgcggcgg acgtaatcat ggttgcgcgc ggtgacttag gtgttgaaat cggtgatccg    780

gaattagtcg gtgttcagaa aaaattaatc cgtcgttcac gtcagttaaa tcgtgttgtt    840

attaccgcaa ctcaaatgat ggaatcaatg attagtaatc ctatgccgac tcgtgcggaa    900

gtaatggacg tagctaacgc agtattggac ggtaccgatg cggtaatgct ttctgctgaa    960

accgcggctg gtcaatatcc ggcggaaact gttgcggcga tggcgaaagt tgcgttaggt   1020

gcggagaaaa tgccaagcat taatgtgtct aaacaccgta tgaacgttca attcgagtct   1080

attgaagaat ctgttgcgat gtctgcaatg tatgcggcaa accacatgag aggcgtagcg   1140

gcgattatca cattaacaag tagcggtcgt actgctcgtt taatgtctag actccatagg   1200

ccgctttcct ggctttgctt ccagatgtat gctctcctcc ggagagtacc gtgactttat   1260

tttcggcaca aatacagggg tcgatggata aatacggcga tagtttcctg acggatgatc   1320

cgtatgtacc ggcggaagac aagctgcaaa cctgtcagat ggagattgat ttaatggcgg   1380

atgtgctgag agcaccgccc cgtgaatccg cagaactgat ccgctatgtg tttgcggatg   1440

attggccgga ataaataaag ccgggcttaa tacagattaa gcccgtatag ggtattatta   1500

ctgaatacca aacagcttac ggaggacgga atgttaccca ttgagacaac cagactgcct   1560

tctgattatt aatatttttc actattaatc agaaggaata accatgaatt ttacccggat   1620

tgacctgaat acctggaatc gcagggaaca ctttgccctt tatcgtcagc agattaaatg   1680

cggattcagc ctgaccacca aactcgatat taccgctttg cgtaccgcac tggcggagac   1740

aggttataag ttttatccgc tgatgattta cctgatctcc cgggctgtta atcagtttcc   1800

ggagttccgg atggcactga aagacaatga acttatttac tgggaccagt cagacccggt   1860

ctttactgtc tttcataaag aaaccgaaac attctctgca ctgtcctgcc gttattttcc   1920

ggatctcagt gagtttatgg caggttataa tgcggtaacg gcagaatatc agcatgatac   1980

cagattgttt ccgcagggaa atttaccgga gaatcacctg aatatatcat cattaccgtg   2040

ggtgagtttt gacgggattt aacctgaaca tcaccggaaa tgatgattat tttgccccgg   2100

tttttacgat ggcaaagttt cagcaggaag gtgaccgcgt attattacct gtttctgtac   2160

aggttcatca tgcagtctgt gatggctttc atgcagcacg gtttattaat acacttcagc   2220

tgatgtgtga taacatactg aaataaatta attaattctg tatttaagcc accgtatccg   2280

gcaggaatgg tggctttttt tttatatttt aaccgtaatc tgtaatttcg tttcagactg   2340

gttcaggatg agctcgcttg gactcctgtt gatagatcca gtaatgacct cagaactcca   2400

tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccaag   2460

cactagcggc gcgccggccg gcccggtgtg aaataccgca cagatgcgta aggagaaaat   2520

accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc   2580

tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg   2640

ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg   2700

ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac   2760

gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg   2820

gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct   2880

ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg   2940

tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct   3000

gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac   3060

tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt   3120

tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc   3180

tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca   3240

ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat   3300

ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac   3360

gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaagg   3420

ccggccgcgg ccgccatcgg cattttcttt tgcgttttta tttgttaact gttaattgtc   3480

cttgttcaag gatgctgtct ttgacaacag atgttttctt gcctttgatg ttcagcagga   3540

agctcggcgc aaacgttgat tgtttgtctg cgtagaatcc tctgtttgtc atatagcttg   3600

taatcacgac attgtttcct ttcgcttgag gtacagcgaa gtgtgagtaa gtaaaggtta   3660

catcgttagg atcaagatcc atttttaaca caaggccagt tttgttcagc ggcttgtatg   3720

ggccagttaa agaattagaa acataaccaa gcatgtaaat atcgttagac gtaatgccgt   3780

caatcgtcat ttttgatccg cgggagtcag tgaacaggta ccatttgccg ttcattttaa   3840

agacgttcgc gcgttcaatt tcatctgtta ctgtgttaga tgcaatcagc ggtttcatca   3900

cttttttcag tgtgtaatca tcgtttagct caatcatacc gagagcgccg tttgctaact   3960

cagccgtgcg ttttttatcg ctttgcagaa gtttttgact ttcttgacgg aagaatgatg   4020

tgcttttgcc atagtatgct ttgttaaata aagattcttc gccttggtag ccatcttcag   4080

ttccagtgtt tgcttcaaat actaagtatt tgtggccttt atcttctacg tagtgaggat   4140

ctctcagcgt atggttgtcg cctgagctgt agttgccttc atcgatgaac tgctgtacat   4200

tttgatacgt ttttccgtca ccgtcaaaga ttgatttata atcctctaca ccgttgatgt   4260

tcaaagagct gtctgatgct gatacgttaa cttgtgcagt tgtcagtgtt tgtttgccgt   4320

aatgtttacc ggagaaatca gtgtagaata aacggatttt tccgtcagat gtaaatgtgg   4380

ctgaacctga ccattcttgt gtttggtctt ttaggataga atcatttgca tcgaatttgt   4440

cgctgtcttt aaagacgcgg ccagcgtttt tccagctgtc aatagaagtt tcgccgactt   4500

tttgatagaa catgtaaatc gatgtgtcat ccgcattttt aggatctccg gctaatgcaa   4560

agacgatgtg gtagccgtga tagtttgcga cagtgccgtc agcgttttgt aatggccagc   4620

tgtcccaaac gtccaggcct tttgcagaag agatattttt aattgtggac gaatcaaatt   4680

cagaaacttg atatttttca tttttttgct gttcagggat ttgcagcata tcatggcgtg   4740

taatatggga aatgccgtat gtttccttat atggcttttg gttcgtttct ttcgcaaacg   4800

cttgagttgc gcctcctgcc agcagtgcgg tagtaaaggt taatactgtt gcttgttttg   4860

caaacttttt gatgttcatc gttcatgtct ccttttttat gtactgtgtt agcggtctgc   4920

ttcttccagc cctcctgttt gaagatggca agttagttac gcacaataaa aaaagaccta   4980

aaatatgtaa ggggtgacgc caaagtatac actttgccct ttacacattt taggtcttgc   5040

ctgctttatc agtaacaaac ccgcgcgatt tacttttcga cctcattcta ttagactctc   5100

gtttggattg caactggtct attttcctct tttgtttgat agaaaatcat aaaaggattt   5160

gcagactacg ggcctaaaga actaaaaaat ctatctgttt cttttcattc tctgtatttt   5220

ttatagtttc tgttgcatgg gcataaagtt gcctttttaa tcacaattca gaaaatatca   5280

taatatctca tttcactaaa taatagtgaa cggcaggtat atgtgatggg ttaaaaagga   5340

tcggcggccg ctcgatttaa atc                                           5363


<210>  14
<211>  5363
<212>  DNA
<213>  artificial sequence

<220>
<223>  complete nucleotide sequence of plasmid pSacB_pykA6

<400>  14
tcgagcagaa gattaagaag aacgaaaatc gtatgtacaa tggggcctgc aacagacaaa     60

ggcaataatt tagaaaaaat cattgctgcc ggtgcaaacg ttgtacgtat gaacttctcc    120

cacggtacgc ccgaagatca tatcggtcgt gctgaaaaag tacgtgaaat cgctcataaa    180

ttaggtaaac acgtagcaat cttaggtgac ttacaaggcc ctaaaatccg tgtttctact    240

tttaaagaag gcaaaatttt cttaaatatc ggtgataaat tcattttaga cgcagagatg    300

cctaaaggtg aaggtaacca ggaagcggtt ggtttagact ataaaacatt accgcaagat    360

gtggttccgg gcgatatctt attattagat gacggtcgag ttcaattgaa agtattggca    420

accgaaggtg caaaagtatt caccgaagta acggtcggtg gcccactatc aaataataaa    480

ggcattaaca aattaggctg cggtttatct gccgatgcgt taaccgaaaa agataaagcg    540

gatatcatta ctgcggcgcg tatcggtgtg gattaccttg ccgtatcttt cccgcgttca    600

agcgcggatt taaactacgc ccgtcaatta gcaaaagatg cgggcttgga tgcgaaaatc    660

gttgcgaaag tagaacgtgc cgaaacagtt gaaacggacg aagcaatgga cgatatcatc    720

aatgcggcgg acgtaatcat ggttgcgcgc ggtgacttag gtgttgaaat cggtgatccg    780

gaattagtcg gtgttcagaa aaaattaatc cgtcgttcac gtcagttaaa tcgtgttgtt    840

attaccgcaa ctcaaatgat ggaatcaatg attagtaatc ctatgccgac tcgtgcggaa    900

gtaatggacg tagctaacgc agtattggac ggtaccgatg cggtaatgct ttctgctgaa    960

accgcggctg gtcaatatcc ggcggaaact gttgcggcga tggcgaaagt tgcgttaggt   1020

gcggagaaaa tgccaagcat taatgtgtct aaacaccgta tgaacgttca attcgagtct   1080

attgaagaat ctgttgcgat gtctgcaatg tatgcggcaa accacatgag aggcgtagcg   1140

gcgattatca cattaacaag tagcggtcgt actgctcgtt taatgtctag actccatagg   1200

ccgctttcct ggctttgctt ccagatgtat gctctcctcc ggagagtacc gtgactttat   1260

tttcggcaca aatacagggg tcgatggata aatacggcga tagtttcctg acggatgatc   1320

cgtatgtacc ggcggaagac aagctgcaaa cctgtcagat ggagattgat ttaatggcgg   1380

atgtgctgag agcaccgccc cgtgaatccg cagaactgat ccgctatgtg tttgcggatg   1440

attggccgga ataaataaag ccgggcttaa tacagattaa gcccgtatag ggtattatta   1500

ctgaatacca aacagcttac ggaggacgga atgttaccca ttgagacaac cagactgcct   1560

tctgattatt aatatttttc actattaatc agaaggaata accatgaatt ttacccggat   1620

tgacctgaat acctggaatc gcagggaaca ctttgccctt tatcgtcagc agattaaatg   1680

cggattcagc ctgaccacca aactcgatat taccgctttg cgtaccgcac tggcggagac   1740

aggttataag ttttatccgc tgatgattta cctgatctcc cgggctgtta atcagtttcc   1800

ggagttccgg atggcactga aagacaatga acttatttac tgggaccagt cagacccggt   1860

ctttactgtc tttcataaag aaaccgaaac attctctgca ctgtcctgcc gttattttcc   1920

ggatctcagt gagtttatgg caggttataa tgcggtaacg gcagaatatc agcatgatac   1980

cagattgttt ccgcagggaa atttaccgga gaatcacctg aatatatcat cattaccgtg   2040

ggtgagtttt gacgggattt aacctgaaca tcaccggaaa tgatgattat tttgccccgg   2100

tttttacgat ggcaaagttt cagcaggaag gtgaccgcgt attattacct gtttctgtac   2160

aggttcatca tgcagtctgt gatggctttc atgcagcacg gtttattaat acacttcagc   2220

tgatgtgtga taacatactg aaataaatta attaattctg tatttaagcc accgtatccg   2280

gcaggaatgg tggctttttt tttatatttt aaccgtaatc tgtaatttcg tttcagactg   2340

gttcaggatg agctcgcttg gactcctgtt gatagatcca gtaatgacct cagaactcca   2400

tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccaag   2460

cactagcggc gcgccggccg gcccggtgtg aaataccgca cagatgcgta aggagaaaat   2520

accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc   2580

tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg   2640

ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg   2700

ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac   2760

gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg   2820

gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct   2880

ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg   2940

tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct   3000

gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac   3060

tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt   3120

tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc   3180

tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca   3240

ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat   3300

ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac   3360

gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaagg   3420

ccggccgcgg ccgccatcgg cattttcttt tgcgttttta tttgttaact gttaattgtc   3480

cttgttcaag gatgctgtct ttgacaacag atgttttctt gcctttgatg ttcagcagga   3540

agctcggcgc aaacgttgat tgtttgtctg cgtagaatcc tctgtttgtc atatagcttg   3600

taatcacgac attgtttcct ttcgcttgag gtacagcgaa gtgtgagtaa gtaaaggtta   3660

catcgttagg atcaagatcc atttttaaca caaggccagt tttgttcagc ggcttgtatg   3720

ggccagttaa agaattagaa acataaccaa gcatgtaaat atcgttagac gtaatgccgt   3780

caatcgtcat ttttgatccg cgggagtcag tgaacaggta ccatttgccg ttcattttaa   3840

agacgttcgc gcgttcaatt tcatctgtta ctgtgttaga tgcaatcagc ggtttcatca   3900

cttttttcag tgtgtaatca tcgtttagct caatcatacc gagagcgccg tttgctaact   3960

cagccgtgcg ttttttatcg ctttgcagaa gtttttgact ttcttgacgg aagaatgatg   4020

tgcttttgcc atagtatgct ttgttaaata aagattcttc gccttggtag ccatcttcag   4080

ttccagtgtt tgcttcaaat actaagtatt tgtggccttt atcttctacg tagtgaggat   4140

ctctcagcgt atggttgtcg cctgagctgt agttgccttc atcgatgaac tgctgtacat   4200

tttgatacgt ttttccgtca ccgtcaaaga ttgatttata atcctctaca ccgttgatgt   4260

tcaaagagct gtctgatgct gatacgttaa cttgtgcagt tgtcagtgtt tgtttgccgt   4320

aatgtttacc ggagaaatca gtgtagaata aacggatttt tccgtcagat gtaaatgtgg   4380

ctgaacctga ccattcttgt gtttggtctt ttaggataga atcatttgca tcgaatttgt   4440

cgctgtcttt aaagacgcgg ccagcgtttt tccagctgtc aatagaagtt tcgccgactt   4500

tttgatagaa catgtaaatc gatgtgtcat ccgcattttt aggatctccg gctaatgcaa   4560

agacgatgtg gtagccgtga tagtttgcga cagtgccgtc agcgttttgt aatggccagc   4620

tgtcccaaac gtccaggcct tttgcagaag agatattttt aattgtggac gaatcaaatt   4680

cagaaacttg atatttttca tttttttgct gttcagggat ttgcagcata tcatggcgtg   4740

taatatggga aatgccgtat gtttccttat atggcttttg gttcgtttct ttcgcaaacg   4800

cttgagttgc gcctcctgcc agcagtgcgg tagtaaaggt taatactgtt gcttgttttg   4860

caaacttttt gatgttcatc gttcatgtct ccttttttat gtactgtgtt agcggtctgc   4920

ttcttccagc cctcctgttt gaagatggca agttagttac gcacaataaa aaaagaccta   4980

aaatatgtaa ggggtgacgc caaagtatac actttgccct ttacacattt taggtcttgc   5040

ctgctttatc agtaacaaac ccgcgcgatt tacttttcga cctcattcta ttagactctc   5100

gtttggattg caactggtct attttcctct tttgtttgat agaaaatcat aaaaggattt   5160

gcagactacg ggcctaaaga actaaaaaat ctatctgttt cttttcattc tctgtatttt   5220

ttatagtttc tgttgcatgg gcataaagtt gcctttttaa tcacaattca gaaaatatca   5280

taatatctca tttcactaaa taatagtgaa cggcaggtat atgtgatggg ttaaaaagga   5340

tcggcggccg ctcgatttaa atc                                           5363


<210>  15
<211>  1440
<212>  DNA
<213>  Basfia succiniciproducens


<220>
<221>  misc_feature
<222>  (1)..(1440)
<223>  nucleotide sequence of the pykA-gene from the DD1 delta ldhA 
       delta pflA pykA2-strain

<400>  15
atgtccagaa gattaagaag aacgaaaatc gtatgtacaa tggggcctgc aacagacaaa     60

ggcaataatt tagaaaaaat cattgctgcc ggtgcaaacg ttgtacgtat gaacttctcc    120

cacggtacgc ccgaagatca tatcggtcgt gctgaaaaag tacgtgaaat cgctcataaa    180

ttaggtaaac acgtagcaat cttaggtgac ttacaaggcc ctaaaatccg tgtttctact    240

tttaaagaag gcaaaatttt cttaaatatc ggtgataaat tcattttaga cgcagagatg    300

cctaaaggtg aaggtaacca ggaagcggtt ggtttagact ataaaacatt accgcaagat    360

gtggttccgg gcgatatctt attattagat gacggtcgag ttcaattgaa agtattggca    420

accgaaggtg caaaagtatt caccgaagta acggtcggtg gcccactatc aaataataaa    480

ggcattaaca aattaggctg cggtttatct gccgatgcat taaccgaaaa agataaagcg    540

gatatcatta ctgcggcgcg tatcggtgtg gattaccttg ccgtatcttt cccgcgttca    600

agcgcggatt taaactacgc ccgtcaatta gcaaaagatg cgggcttgga tgcgaaaatc    660

gttgcgaaag tagaacgtgc cgaaacagtt gaaacggacg aagcaatgga cgatatcatc    720

aatgcggcgg acgtaatcat ggttgcgcgc ggtgacttag gtgttgaaat cggtgatccg    780

gaattagtcg gtgttcagaa aaaattaatc cgtcgttcac gtcagttaaa tcgtgttgtt    840

attaccgcaa ctcaaatgat ggaatcaatg attagtaatc ctatgccgac tcgtgcggaa    900

gtaatggacg tagctaacgc agtattggac ggtaccgatg cggtaatgct ttctgctgaa    960

accgcggctg gtcaatatcc ggcggaaact gttgcggcga tggcgaaagt tgcgttaggt   1020

gcggagaaaa tgccaagcat taatgtgtct aaacaccgta tgaacgttca attcgagtct   1080

attgaagaat ctgttgcgat gtctgcaatg tatgcggcaa accacatgag aggcgtagcg   1140

gcgattatca cattaacaag tagcggtcgt actgctcgtt taatgtctcg cattagttcc   1200

ggtttaccaa tctttgcatt gtcacgtaac gaatctacat taaacttatg cgcattatat   1260

cgtggtgtga caccggttca ttttgataaa gacagccgta cctcagaagg tgcgacagcg   1320

gcggttcaat tattaaaaga cgaaggtttc ttagtgtctg gcgatttagt gttattaact   1380

cagggcgacg caagcagttc tagcggtact aacctttgcc gtacattgat tgttgaataa   1440


<210>  16
<211>  479
<212>  PRT
<213>  Basfia succiniciproducens


<220>
<221>  SITE
<222>  (1)..(479)
<223>  amino acid sequence of PykA2 from the DD1 delta ldhA delta pflA 
       pykA2-strain

<400>  16

Met Ser Arg Arg Leu Arg Arg Thr Lys Ile Val Cys Thr Met Gly Pro 
1               5                   10                  15      


Ala Thr Asp Lys Gly Asn Asn Leu Glu Lys Ile Ile Ala Ala Gly Ala 
            20                  25                  30          


Asn Val Val Arg Met Asn Phe Ser His Gly Thr Pro Glu Asp His Ile 
        35                  40                  45              


Gly Arg Ala Glu Lys Val Arg Glu Ile Ala His Lys Leu Gly Lys His 
    50                  55                  60                  


Val Ala Ile Leu Gly Asp Leu Gln Gly Pro Lys Ile Arg Val Ser Thr 
65                  70                  75                  80  


Phe Lys Glu Gly Lys Ile Phe Leu Asn Ile Gly Asp Lys Phe Ile Leu 
                85                  90                  95      


Asp Ala Glu Met Pro Lys Gly Glu Gly Asn Gln Glu Ala Val Gly Leu 
            100                 105                 110         


Asp Tyr Lys Thr Leu Pro Gln Asp Val Val Pro Gly Asp Ile Leu Leu 
        115                 120                 125             


Leu Asp Asp Gly Arg Val Gln Leu Lys Val Leu Ala Thr Glu Gly Ala 
    130                 135                 140                 


Lys Val Phe Thr Glu Val Thr Val Gly Gly Pro Leu Ser Asn Asn Lys 
145                 150                 155                 160 


Gly Ile Asn Lys Leu Gly Cys Gly Leu Ser Ala Asp Ala Leu Thr Glu 
                165                 170                 175     


Lys Asp Lys Ala Asp Ile Ile Thr Ala Ala Arg Ile Gly Val Asp Tyr 
            180                 185                 190         


Leu Ala Val Ser Phe Pro Arg Ser Ser Ala Asp Leu Asn Tyr Ala Arg 
        195                 200                 205             


Gln Leu Ala Lys Asp Ala Gly Leu Asp Ala Lys Ile Val Ala Lys Val 
    210                 215                 220                 


Glu Arg Ala Glu Thr Val Glu Thr Asp Glu Ala Met Asp Asp Ile Ile 
225                 230                 235                 240 


Asn Ala Ala Asp Val Ile Met Val Ala Arg Gly Asp Leu Gly Val Glu 
                245                 250                 255     


Ile Gly Asp Pro Glu Leu Val Gly Val Gln Lys Lys Leu Ile Arg Arg 
            260                 265                 270         


Ser Arg Gln Leu Asn Arg Val Val Ile Thr Ala Thr Gln Met Met Glu 
        275                 280                 285             


Ser Met Ile Ser Asn Pro Met Pro Thr Arg Ala Glu Val Met Asp Val 
    290                 295                 300                 


Ala Asn Ala Val Leu Asp Gly Thr Asp Ala Val Met Leu Ser Ala Glu 
305                 310                 315                 320 


Thr Ala Ala Gly Gln Tyr Pro Ala Glu Thr Val Ala Ala Met Ala Lys 
                325                 330                 335     


Val Ala Leu Gly Ala Glu Lys Met Pro Ser Ile Asn Val Ser Lys His 
            340                 345                 350         


Arg Met Asn Val Gln Phe Glu Ser Ile Glu Glu Ser Val Ala Met Ser 
        355                 360                 365             


Ala Met Tyr Ala Ala Asn His Met Arg Gly Val Ala Ala Ile Ile Thr 
    370                 375                 380                 


Leu Thr Ser Ser Gly Arg Thr Ala Arg Leu Met Ser Arg Ile Ser Ser 
385                 390                 395                 400 


Gly Leu Pro Ile Phe Ala Leu Ser Arg Asn Glu Ser Thr Leu Asn Leu 
                405                 410                 415     


Cys Ala Leu Tyr Arg Gly Val Thr Pro Val His Phe Asp Lys Asp Ser 
            420                 425                 430         


Arg Thr Ser Glu Gly Ala Thr Ala Ala Val Gln Leu Leu Lys Asp Glu 
        435                 440                 445             


Gly Phe Leu Val Ser Gly Asp Leu Val Leu Leu Thr Gln Gly Asp Ala 
    450                 455                 460                 


Ser Ser Ser Ser Gly Thr Asn Leu Cys Arg Thr Leu Ile Val Glu 
465                 470                 475                 


<210>  17
<211>  1440
<212>  DNA
<213>  Basfia succiniciproducens


<220>
<221>  misc_feature
<222>  (1)..(1440)
<223>  nucleotide sequence of the pykA-gene from the DD1 delta ldhA 
       delta pflD pykA4-strain

<400>  17
atgtccagaa gattaagaag aacgaaaatc gtatgtacaa tggggcctgc aacagacaaa     60

ggcaataatt tagaaaaaat cattgctgcc ggtgcaaacg ttgtacgtat gaacttctcc    120

cacggtacgc ccgaagatca tatcggtcgt gctgaaaaag tacgtgaaat cgctcataaa    180

ttaggtaaac acgtagcaat cttaggtgac ttacaaggcc ctaaaatccg tgtttctact    240

tttaaagaag gcaaaatttt cttaaatatc ggtgataaat tcattttaga cgcagagatg    300

cctaaaggtg aaggtaacca ggaagcggtt ggtttagact ataaaacatt accgcaagat    360

gtggttccgg gcgatatctt attattagat gacggtcgag ttcaattgaa agtattggca    420

accgaaggtg caaaagtatt caccgaagta acggtcggtg gcccactatc aaataataaa    480

ggcattaaca aattaggcgg cggtttatct gccgatgcat taaccgaaaa agataaagcg    540

gatatcatta ctgcggcgcg tatcggtgtg gattaccttg ccgtatcttt cccgcgttca    600

agcgcggatt taaactacgc ccgtcaatta gcaaaagatg cgggcttgga tgcgaaaatc    660

gttgcgaaag tagaacgtgc cgaaacagtt gaaacggacg aagcaatgga cgatatcatc    720

aatgcggcgg acgtaatcat ggttgcgcgc ggtgacttag gtgttgaaat cggtgatccg    780

gaattagtcg gtgttcagaa aaaattaatc cgtcgttcac gtcagttaaa tcgtgttgtt    840

attaccgcaa ctcaaatgat ggaatcaatg attagtaatc ctatgccgac tcgtgcggaa    900

gtaatggacg tagctaacgc agtattggac ggtaccgatg cggtaatgct ttctgctgaa    960

accgcggctg gtcaatatcc ggcggaaact gttgcggcga tggcgaaagt tgcgttaggt   1020

gcggagaaaa tgccaagcat taatgtgtct aaacaccgta tgaacgttca attcgagtct   1080

attgaagaat ctgttgcgat gtctgcaatg tatgcggcaa accacatgag aggcgtagcg   1140

gcgattatca cattaacaag tagcggtcgt actgctcgtt taatgtctcg cattagttcc   1200

ggtttaccaa tctttgcatt gtcacgtaac gaatctactt taaacttata cgcattatat   1260

cgtggtgtga caccggttca ttttgataaa gacagccgta cctcagaagg tgcgacagcg   1320

gcggttcaat tattaaaaga cgaaggtttc ttagtgtctg gcgatttagt gttattaact   1380

cagggcgacg caagcagttc tagcggtact aacctttgcc gtacattgat tgttgaataa   1440


<210>  18
<211>  479
<212>  PRT
<213>  Basfia succiniciproducens


<220>
<221>  SITE
<222>  (1)..(479)
<223>  amino acid sequence of PykA4 from the DD1 delta ldhA delta pflD 
       pykA4-strain

<400>  18

Met Ser Arg Arg Leu Arg Arg Thr Lys Ile Val Cys Thr Met Gly Pro 
1               5                   10                  15      


Ala Thr Asp Lys Gly Asn Asn Leu Glu Lys Ile Ile Ala Ala Gly Ala 
            20                  25                  30          


Asn Val Val Arg Met Asn Phe Ser His Gly Thr Pro Glu Asp His Ile 
        35                  40                  45              


Gly Arg Ala Glu Lys Val Arg Glu Ile Ala His Lys Leu Gly Lys His 
    50                  55                  60                  


Val Ala Ile Leu Gly Asp Leu Gln Gly Pro Lys Ile Arg Val Ser Thr 
65                  70                  75                  80  


Phe Lys Glu Gly Lys Ile Phe Leu Asn Ile Gly Asp Lys Phe Ile Leu 
                85                  90                  95      


Asp Ala Glu Met Pro Lys Gly Glu Gly Asn Gln Glu Ala Val Gly Leu 
            100                 105                 110         


Asp Tyr Lys Thr Leu Pro Gln Asp Val Val Pro Gly Asp Ile Leu Leu 
        115                 120                 125             


Leu Asp Asp Gly Arg Val Gln Leu Lys Val Leu Ala Thr Glu Gly Ala 
    130                 135                 140                 


Lys Val Phe Thr Glu Val Thr Val Gly Gly Pro Leu Ser Asn Asn Lys 
145                 150                 155                 160 


Gly Ile Asn Lys Leu Gly Gly Gly Leu Ser Ala Asp Ala Leu Thr Glu 
                165                 170                 175     


Lys Asp Lys Ala Asp Ile Ile Thr Ala Ala Arg Ile Gly Val Asp Tyr 
            180                 185                 190         


Leu Ala Val Ser Phe Pro Arg Ser Ser Ala Asp Leu Asn Tyr Ala Arg 
        195                 200                 205             


Gln Leu Ala Lys Asp Ala Gly Leu Asp Ala Lys Ile Val Ala Lys Val 
    210                 215                 220                 


Glu Arg Ala Glu Thr Val Glu Thr Asp Glu Ala Met Asp Asp Ile Ile 
225                 230                 235                 240 


Asn Ala Ala Asp Val Ile Met Val Ala Arg Gly Asp Leu Gly Val Glu 
                245                 250                 255     


Ile Gly Asp Pro Glu Leu Val Gly Val Gln Lys Lys Leu Ile Arg Arg 
            260                 265                 270         


Ser Arg Gln Leu Asn Arg Val Val Ile Thr Ala Thr Gln Met Met Glu 
        275                 280                 285             


Ser Met Ile Ser Asn Pro Met Pro Thr Arg Ala Glu Val Met Asp Val 
    290                 295                 300                 


Ala Asn Ala Val Leu Asp Gly Thr Asp Ala Val Met Leu Ser Ala Glu 
305                 310                 315                 320 


Thr Ala Ala Gly Gln Tyr Pro Ala Glu Thr Val Ala Ala Met Ala Lys 
                325                 330                 335     


Val Ala Leu Gly Ala Glu Lys Met Pro Ser Ile Asn Val Ser Lys His 
            340                 345                 350         


Arg Met Asn Val Gln Phe Glu Ser Ile Glu Glu Ser Val Ala Met Ser 
        355                 360                 365             


Ala Met Tyr Ala Ala Asn His Met Arg Gly Val Ala Ala Ile Ile Thr 
    370                 375                 380                 


Leu Thr Ser Ser Gly Arg Thr Ala Arg Leu Met Ser Arg Ile Ser Ser 
385                 390                 395                 400 


Gly Leu Pro Ile Phe Ala Leu Ser Arg Asn Glu Ser Thr Leu Asn Leu 
                405                 410                 415     


Tyr Ala Leu Tyr Arg Gly Val Thr Pro Val His Phe Asp Lys Asp Ser 
            420                 425                 430         


Arg Thr Ser Glu Gly Ala Thr Ala Ala Val Gln Leu Leu Lys Asp Glu 
        435                 440                 445             


Gly Phe Leu Val Ser Gly Asp Leu Val Leu Leu Thr Gln Gly Asp Ala 
    450                 455                 460                 


Ser Ser Ser Ser Gly Thr Asn Leu Cys Arg Thr Leu Ile Val Glu 
465                 470                 475                 


<210>  19
<211>  1440
<212>  DNA
<213>  Basfia succiniciproducens


<220>
<221>  misc_feature
<222>  (1)..(1440)
<223>  nucleotide sequence of the pykA-gene from the DD1 delta ldhA 
       delta pflD pykA5-strain

<400>  19
atgtccagaa gattaagaag aacgaaaatc gtatgtacaa tggggcctgc aacagacaaa     60

ggcaataatt tagaaaaaat cattgctgcc ggtgcaaacg ttgtacgtat gaacttctcc    120

cacggtacgc ccgaagatca tatcggtcgt gctgaaaaag tacgtgaaat cgctcataaa    180

ttaggtaaac acgtagcaat cttaggtgac ttacaaggcc ctaaaatccg tgtttctact    240

tttaaagaag gcaaaatttt cttaaatatc ggtgataaat tcattttaga cgcagagatg    300

cctaaaggtg aaggtaacca ggaagcggtt ggtttagact ataaaacatt accgcaagat    360

gtggttccgg gcgatatctt attattagat gacggtcgag ttcaattgaa agtattggca    420

accgaaggtg caaaagtatt caccgaagta acggtcggtg gcccactatc aaataataaa    480

ggcattaaca aattaggcgg cggtttatct ggcgatgcgt taaccgaaaa agataaagcg    540

gatatcatta ctgcggcgcg tatcggtgtg gattaccttg ccgtatcttt cccgcgttca    600

agcgcggatt taaactacgc ccgtcaatta gcaaaagatg cgggcttgga tgcgaaaatc    660

gttgcgaaag tagaacgtgc cgaaacagtt gaaacggacg aagcaatgga cgatatcatc    720

aatgcggcgg acgtaatcat ggttgcgcgc ggtgacttag gtgttgaaat cggtgatccg    780

gaattagtcg gtgttcagaa aaaattaatc cgtcgttcac gtcagttaaa tcgtgttgtt    840

attaccgcaa ctcaaatgat ggaatcaatg attagtaatc ctatgccgac tcgtgcggaa    900

gtaatggacg tagctaacgc agtattggac ggtaccgatg cggtaatgct ttctgctgaa    960

accgcggctg gtcaatatcc ggcggaaact gttgcggcga tggcgaaagt tgcgttaggt   1020

gcggagaaaa tgccaagcat taatgtgtct aaacaccgta tgaacgttca attcgagtct   1080

attgaagaat ctgttgcgat gtctgcaatg tatgcggcaa accacatgag aggcgtagcg   1140

gcgattatca cattaacaag tagcggtcgt actgctcgtt taatgtctcg cattagttcc   1200

ggtttaccaa tctttgcatt gtcacgtaac gaatctacat taaacttatg cgcattatat   1260

cgtggtgtga caccggttca ttttgataaa gacagccgta cctcagaagg tgcgacagcg   1320

gcggttcaat tattaaaaga cgaaggtttc ttagtgtctg gcgatttagt gttattaact   1380

cagggcgacg caagcagttc tagcggtact aacctttgcc gtacattgat tgttgaataa   1440


<210>  20
<211>  479
<212>  PRT
<213>  Basfia succiniciproducens


<220>
<221>  SITE
<222>  (1)..(479)
<223>  amino acid sequence of PykA5 from the DD1 delta ldhA delta pflD 
       pykA5-strain

<400>  20

Met Ser Arg Arg Leu Arg Arg Thr Lys Ile Val Cys Thr Met Gly Pro 
1               5                   10                  15      


Ala Thr Asp Lys Gly Asn Asn Leu Glu Lys Ile Ile Ala Ala Gly Ala 
            20                  25                  30          


Asn Val Val Arg Met Asn Phe Ser His Gly Thr Pro Glu Asp His Ile 
        35                  40                  45              


Gly Arg Ala Glu Lys Val Arg Glu Ile Ala His Lys Leu Gly Lys His 
    50                  55                  60                  


Val Ala Ile Leu Gly Asp Leu Gln Gly Pro Lys Ile Arg Val Ser Thr 
65                  70                  75                  80  


Phe Lys Glu Gly Lys Ile Phe Leu Asn Ile Gly Asp Lys Phe Ile Leu 
                85                  90                  95      


Asp Ala Glu Met Pro Lys Gly Glu Gly Asn Gln Glu Ala Val Gly Leu 
            100                 105                 110         


Asp Tyr Lys Thr Leu Pro Gln Asp Val Val Pro Gly Asp Ile Leu Leu 
        115                 120                 125             


Leu Asp Asp Gly Arg Val Gln Leu Lys Val Leu Ala Thr Glu Gly Ala 
    130                 135                 140                 


Lys Val Phe Thr Glu Val Thr Val Gly Gly Pro Leu Ser Asn Asn Lys 
145                 150                 155                 160 


Gly Ile Asn Lys Leu Gly Gly Gly Leu Ser Gly Asp Ala Leu Thr Glu 
                165                 170                 175     


Lys Asp Lys Ala Asp Ile Ile Thr Ala Ala Arg Ile Gly Val Asp Tyr 
            180                 185                 190         


Leu Ala Val Ser Phe Pro Arg Ser Ser Ala Asp Leu Asn Tyr Ala Arg 
        195                 200                 205             


Gln Leu Ala Lys Asp Ala Gly Leu Asp Ala Lys Ile Val Ala Lys Val 
    210                 215                 220                 


Glu Arg Ala Glu Thr Val Glu Thr Asp Glu Ala Met Asp Asp Ile Ile 
225                 230                 235                 240 


Asn Ala Ala Asp Val Ile Met Val Ala Arg Gly Asp Leu Gly Val Glu 
                245                 250                 255     


Ile Gly Asp Pro Glu Leu Val Gly Val Gln Lys Lys Leu Ile Arg Arg 
            260                 265                 270         


Ser Arg Gln Leu Asn Arg Val Val Ile Thr Ala Thr Gln Met Met Glu 
        275                 280                 285             


Ser Met Ile Ser Asn Pro Met Pro Thr Arg Ala Glu Val Met Asp Val 
    290                 295                 300                 


Ala Asn Ala Val Leu Asp Gly Thr Asp Ala Val Met Leu Ser Ala Glu 
305                 310                 315                 320 


Thr Ala Ala Gly Gln Tyr Pro Ala Glu Thr Val Ala Ala Met Ala Lys 
                325                 330                 335     


Val Ala Leu Gly Ala Glu Lys Met Pro Ser Ile Asn Val Ser Lys His 
            340                 345                 350         


Arg Met Asn Val Gln Phe Glu Ser Ile Glu Glu Ser Val Ala Met Ser 
        355                 360                 365             


Ala Met Tyr Ala Ala Asn His Met Arg Gly Val Ala Ala Ile Ile Thr 
    370                 375                 380                 


Leu Thr Ser Ser Gly Arg Thr Ala Arg Leu Met Ser Arg Ile Ser Ser 
385                 390                 395                 400 


Gly Leu Pro Ile Phe Ala Leu Ser Arg Asn Glu Ser Thr Leu Asn Leu 
                405                 410                 415     


Cys Ala Leu Tyr Arg Gly Val Thr Pro Val His Phe Asp Lys Asp Ser 
            420                 425                 430         


Arg Thr Ser Glu Gly Ala Thr Ala Ala Val Gln Leu Leu Lys Asp Glu 
        435                 440                 445             


Gly Phe Leu Val Ser Gly Asp Leu Val Leu Leu Thr Gln Gly Asp Ala 
    450                 455                 460                 


Ser Ser Ser Ser Gly Thr Asn Leu Cys Arg Thr Leu Ile Val Glu 
465                 470                 475                 


<210>  21
<211>  1440
<212>  DNA
<213>  Basfia succiniciproducens


<220>
<221>  misc_feature
<222>  (1)..(1440)
<223>  nucleotide sequence of the pykA-gene from the DD1 delta ldhA 
       delta pflD pykA6-strain

<400>  21
atgtccagaa gattaagaag aacgaaaatc gtatgtacaa tggggcctgc aacagacaaa     60

ggcaataatt tagaaaaaat cattgctgcc ggtgcaaacg ttgtacgtat gaacttctcc    120

cacggtacgc ccgaagatca tatcggtcgt gctgaaaaag tacgtgaaat cgctcataaa    180

ttaggtaaac acgtagcaat cttaggtgac ttacaaggcc ctaaaatccg tgtttctact    240

tttaaagaag gcaaaatttt cttaaatatc ggtgataaat tcattttaga cgcagagatg    300

cctaaaggtg aaggtaacca ggaagcggtt ggtttagact ataaaacatt accgcaagat    360

gtggttccgg gcgatatctt attattagat gacggtcgag ttcaattgaa agtattggca    420

accgaaggtg caaaagtatt caccgaagta acggtcggtg gcccactatc aaataataaa    480

ggcattaaca aattaggctg cggtttatct gccgatgcgt taaccgaaaa agataaagcg    540

gatatcatta ctgcggcgcg tatcggtgtg gattaccttg ccgtatcttt cccgcgttca    600

agcgcggatt taaactacgc ccgtcaatta gcaaaagatg cgggcttgga tgcgaaaatc    660

gttgcgaaag tagaacgtgc cgaaacagtt gaaacggacg aagcaatgga cgatatcatc    720

aatgcggcgg acgtaatcat ggttgcgcgc ggtgacttag gtgttgaaat cggtgatccg    780

gaattagtcg gtgttcagaa aaaattaatc cgtcgttcac gtcagttaaa tcgtgttgtt    840

attaccgcaa ctcaaatgat ggaatcaatg attagtaatc ctatgccgac tcgtgcggaa    900

gtaatggacg tagctaacgc agtattggac ggtaccgatg cggtaatgct ttctgctgaa    960

accgcggctg gtcaatatcc ggcggaaact gttgcggcga tggcgaaagt tgcgttaggt   1020

gcggagaaaa tgccaagcat taatgtgtct aaacaccgta tgaacgttca attcgagtct   1080

attgaagaat ctgttgcgat gtctgcaatg tatgcggcaa accacatgag aggcgtagcg   1140

gcgattatca cattaacaag tagcggtcgt actgctcgtt taatgtctcg cattagttcc   1200

ggtttaccaa tctttgcatt gtcacgtaac gaatctacat taaacttatg cgcattatat   1260

cgtggtgtga caccggttca ttttgataaa gacagccgta cctcagaagg tgcgacagcg   1320

gcggttcaat tattaaaaga cgaaggtttc ttagtgtctg gcgatttagt gttattaact   1380

cagggcgacg caagcagttc tagcggtact aacctttgcc gtacattgat tgttgaataa   1440


<210>  22
<211>  479
<212>  PRT
<213>  Basfia succiniciproducens


<220>
<221>  SITE
<222>  (1)..(479)
<223>  amino acid sequence of PykA6 from the DD1 delta ldhA delta pflD 
       pykA6-strain

<400>  22

Met Ser Arg Arg Leu Arg Arg Thr Lys Ile Val Cys Thr Met Gly Pro 
1               5                   10                  15      


Ala Thr Asp Lys Gly Asn Asn Leu Glu Lys Ile Ile Ala Ala Gly Ala 
            20                  25                  30          


Asn Val Val Arg Met Asn Phe Ser His Gly Thr Pro Glu Asp His Ile 
        35                  40                  45              


Gly Arg Ala Glu Lys Val Arg Glu Ile Ala His Lys Leu Gly Lys His 
    50                  55                  60                  


Val Ala Ile Leu Gly Asp Leu Gln Gly Pro Lys Ile Arg Val Ser Thr 
65                  70                  75                  80  


Phe Lys Glu Gly Lys Ile Phe Leu Asn Ile Gly Asp Lys Phe Ile Leu 
                85                  90                  95      


Asp Ala Glu Met Pro Lys Gly Glu Gly Asn Gln Glu Ala Val Gly Leu 
            100                 105                 110         


Asp Tyr Lys Thr Leu Pro Gln Asp Val Val Pro Gly Asp Ile Leu Leu 
        115                 120                 125             


Leu Asp Asp Gly Arg Val Gln Leu Lys Val Leu Ala Thr Glu Gly Ala 
    130                 135                 140                 


Lys Val Phe Thr Glu Val Thr Val Gly Gly Pro Leu Ser Asn Asn Lys 
145                 150                 155                 160 


Gly Ile Asn Lys Leu Gly Cys Gly Leu Ser Ala Asp Ala Leu Thr Glu 
                165                 170                 175     


Lys Asp Lys Ala Asp Ile Ile Thr Ala Ala Arg Ile Gly Val Asp Tyr 
            180                 185                 190         


Leu Ala Val Ser Phe Pro Arg Ser Ser Ala Asp Leu Asn Tyr Ala Arg 
        195                 200                 205             


Gln Leu Ala Lys Asp Ala Gly Leu Asp Ala Lys Ile Val Ala Lys Val 
    210                 215                 220                 


Glu Arg Ala Glu Thr Val Glu Thr Asp Glu Ala Met Asp Asp Ile Ile 
225                 230                 235                 240 


Asn Ala Ala Asp Val Ile Met Val Ala Arg Gly Asp Leu Gly Val Glu 
                245                 250                 255     


Ile Gly Asp Pro Glu Leu Val Gly Val Gln Lys Lys Leu Ile Arg Arg 
            260                 265                 270         


Ser Arg Gln Leu Asn Arg Val Val Ile Thr Ala Thr Gln Met Met Glu 
        275                 280                 285             


Ser Met Ile Ser Asn Pro Met Pro Thr Arg Ala Glu Val Met Asp Val 
    290                 295                 300                 


Ala Asn Ala Val Leu Asp Gly Thr Asp Ala Val Met Leu Ser Ala Glu 
305                 310                 315                 320 


Thr Ala Ala Gly Gln Tyr Pro Ala Glu Thr Val Ala Ala Met Ala Lys 
                325                 330                 335     


Val Ala Leu Gly Ala Glu Lys Met Pro Ser Ile Asn Val Ser Lys His 
            340                 345                 350         


Arg Met Asn Val Gln Phe Glu Ser Ile Glu Glu Ser Val Ala Met Ser 
        355                 360                 365             


Ala Met Tyr Ala Ala Asn His Met Arg Gly Val Ala Ala Ile Ile Thr 
    370                 375                 380                 


Leu Thr Ser Ser Gly Arg Thr Ala Arg Leu Met Ser Arg Ile Ser Ser 
385                 390                 395                 400 


Gly Leu Pro Ile Phe Ala Leu Ser Arg Asn Glu Ser Thr Leu Asn Leu 
                405                 410                 415     


Cys Ala Leu Tyr Arg Gly Val Thr Pro Val His Phe Asp Lys Asp Ser 
            420                 425                 430         


Arg Thr Ser Glu Gly Ala Thr Ala Ala Val Gln Leu Leu Lys Asp Glu 
        435                 440                 445             


Gly Phe Leu Val Ser Gly Asp Leu Val Leu Leu Thr Gln Gly Asp Ala 
    450                 455                 460                 


Ser Ser Ser Ser Gly Thr Asn Leu Cys Arg Thr Leu Ile Val Glu 
465                 470                 475                 


<210>  23
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  sequence of the pykA4_fw primer

<400>  23
aatgcggcgg acgtaatcat                                                 20


<210>  24
<211>  25
<212>  DNA
<213>  artificial sequence

<220>
<223>  sequence of the pykA4_rv primer

<400>  24
tggagaacct atccatgaag tatca                                           25


<210>  25
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  sequence of the pykA5/6_fw primer

<400>  25
tggggcctgc aacagacaaa                                                 20


<210>  26
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  sequence of the pykA5/6_rv primer

<400>  26
aaacgagcag tacgaccgct                                                 20


<210>  27
<211>  1029
<212>  DNA
<213>  Basfia succiniciproducens


<220>
<221>  misc_feature
<222>  (1)..(1029)
<223>  nucleotide sequence of ldhA-gene from strain DD1

<400>  27
ttgacaaaat cagtatgttt aaataaggag ctaactatga aagttgccgt ttacagtact     60

aaaaattatg atcgcaaaca tctggatttg gcgaataaaa aatttaattt tgagcttcat    120

ttctttgatt ttttacttga tgaacaaacc gcgaaaatgg cggagggcgc cgatgccgtc    180

tgtattttcg tcaatgatga tgcgagccgc ccggtgttaa caaagttggc gcaaatcgga    240

gtgaaaatta tcgctttacg ttgtgccggt tttaataatg tggatttgga ggcggcaaaa    300

gagctgggat taaaagtcgt acgggtgcct gcgtattcgc cggaagccgt tgccgagcat    360

gcgatcggat taatgctgac tttaaaccgc cgtatccata aggcttatca gcgtacccgc    420

gatgcgaatt tttctctgga aggattggtc ggttttaata tgttcggcaa aaccgccgga    480

gtgattggta cgggaaaaat cggcttggcg gctattcgca ttttaaaagg cttcggtatg    540

gacgttctgg cgtttgatcc ttttaaaaat ccggcggcgg aagcgttggg cgcaaaatat    600

gtcggtttag acgagcttta tgcaaaatcc catgttatca ctttgcattg cccggctacg    660

gcggataatt atcatttatt aaatgaagcg gcttttaata aaatgcgcga cggtgtaatg    720

attattaata ccagccgcgg cgttttaatt gacagccggg cggcaatcga agcgttaaaa    780

cggcagaaaa tcggcgctct cggtatggat gtttatgaaa atgaacggga tttgtttttc    840

gaggataaat ctaacgatgt tattacggat gatgtattcc gtcgcctttc ttcctgtcat    900

aatgtgcttt ttaccggtca tcaggcgttt ttaacggaag aagcgctgaa taatatcgcc    960

gatgtgactt tatcgaatat tcaggcggtt tccaaaaatg caacgtgcga aaatagcgtt   1020

gaaggctaa                                                           1029


<210>  28
<211>  342
<212>  PRT
<213>  Basfia succiniciproducens


<220>
<221>  SITE
<222>  (1)..(342)
<223>  amino acid sequence of LdhA from strain DD1

<400>  28

Met Thr Lys Ser Val Cys Leu Asn Lys Glu Leu Thr Met Lys Val Ala 
1               5                   10                  15      


Val Tyr Ser Thr Lys Asn Tyr Asp Arg Lys His Leu Asp Leu Ala Asn 
            20                  25                  30          


Lys Lys Phe Asn Phe Glu Leu His Phe Phe Asp Phe Leu Leu Asp Glu 
        35                  40                  45              


Gln Thr Ala Lys Met Ala Glu Gly Ala Asp Ala Val Cys Ile Phe Val 
    50                  55                  60                  


Asn Asp Asp Ala Ser Arg Pro Val Leu Thr Lys Leu Ala Gln Ile Gly 
65                  70                  75                  80  


Val Lys Ile Ile Ala Leu Arg Cys Ala Gly Phe Asn Asn Val Asp Leu 
                85                  90                  95      


Glu Ala Ala Lys Glu Leu Gly Leu Lys Val Val Arg Val Pro Ala Tyr 
            100                 105                 110         


Ser Pro Glu Ala Val Ala Glu His Ala Ile Gly Leu Met Leu Thr Leu 
        115                 120                 125             


Asn Arg Arg Ile His Lys Ala Tyr Gln Arg Thr Arg Asp Ala Asn Phe 
    130                 135                 140                 


Ser Leu Glu Gly Leu Val Gly Phe Asn Met Phe Gly Lys Thr Ala Gly 
145                 150                 155                 160 


Val Ile Gly Thr Gly Lys Ile Gly Leu Ala Ala Ile Arg Ile Leu Lys 
                165                 170                 175     


Gly Phe Gly Met Asp Val Leu Ala Phe Asp Pro Phe Lys Asn Pro Ala 
            180                 185                 190         


Ala Glu Ala Leu Gly Ala Lys Tyr Val Gly Leu Asp Glu Leu Tyr Ala 
        195                 200                 205             


Lys Ser His Val Ile Thr Leu His Cys Pro Ala Thr Ala Asp Asn Tyr 
    210                 215                 220                 


His Leu Leu Asn Glu Ala Ala Phe Asn Lys Met Arg Asp Gly Val Met 
225                 230                 235                 240 


Ile Ile Asn Thr Ser Arg Gly Val Leu Ile Asp Ser Arg Ala Ala Ile 
                245                 250                 255     


Glu Ala Leu Lys Arg Gln Lys Ile Gly Ala Leu Gly Met Asp Val Tyr 
            260                 265                 270         


Glu Asn Glu Arg Asp Leu Phe Phe Glu Asp Lys Ser Asn Asp Val Ile 
        275                 280                 285             


Thr Asp Asp Val Phe Arg Arg Leu Ser Ser Cys His Asn Val Leu Phe 
    290                 295                 300                 


Thr Gly His Gln Ala Phe Leu Thr Glu Glu Ala Leu Asn Asn Ile Ala 
305                 310                 315                 320 


Asp Val Thr Leu Ser Asn Ile Gln Ala Val Ser Lys Asn Ala Thr Cys 
                325                 330                 335     


Glu Asn Ser Val Glu Gly 
            340         


<210>  29
<211>  741
<212>  DNA
<213>  Basfia succiniciproducens


<220>
<221>  misc_feature
<222>  (1)..(741)
<223>  nucleotide sequence of pflA-gene from strain DD1

<400>  29
atgtcggttt taggacgaat tcattcattt gaaacctgcg ggacagttga cgggccggga     60

atccgcttta ttttattttt acaaggctgc ttaatgcgtt gtaaatactg ccataataga    120

gacacctggg atttgcacgg cggtaaagaa atttccgttg aagaattaat gaaagaagtg    180

gtgacctatc gccattttat gaacgcctcg ggcggcggag ttaccgcttc cggcggtgaa    240

gctattttac aggcggaatt tgtacgggac tggttcagag cctgccataa agaaggaatt    300

aatacttgct tggataccaa cggtttcgtc cgtcatcatg atcatattat tgatgaattg    360

attgatgaca cggatcttgt gttgcttgac ctgaaagaaa tgaatgaacg ggttcacgaa    420

agcctgattg gcgtgccgaa taaaagagtg ctcgaattcg caaaatattt agcggatcga    480

aatcagcgta cctggatccg ccatgttgta gtgccgggtt atacagatag tgacgaagat    540

ttgcacatgc tggggaattt cattaaagat atgaagaata tcgaaaaagt ggaattatta    600

ccttatcacc gtctaggcgc ccataaatgg gaagtactcg gcgataaata cgagcttgaa    660

gatgtaaaac cgccgacaaa agaattaatg gagcatgtta aggggttgct tgcaggctac    720

gggcttaatg tgacatatta g                                              741


<210>  30
<211>  246
<212>  PRT
<213>  Basfia succiniciproducens


<220>
<221>  SITE
<222>  (1)..(246)
<223>  amino acid sequence of PflA from strain DD1

<400>  30

Met Ser Val Leu Gly Arg Ile His Ser Phe Glu Thr Cys Gly Thr Val 
1               5                   10                  15      


Asp Gly Pro Gly Ile Arg Phe Ile Leu Phe Leu Gln Gly Cys Leu Met 
            20                  25                  30          


Arg Cys Lys Tyr Cys His Asn Arg Asp Thr Trp Asp Leu His Gly Gly 
        35                  40                  45              


Lys Glu Ile Ser Val Glu Glu Leu Met Lys Glu Val Val Thr Tyr Arg 
    50                  55                  60                  


His Phe Met Asn Ala Ser Gly Gly Gly Val Thr Ala Ser Gly Gly Glu 
65                  70                  75                  80  


Ala Ile Leu Gln Ala Glu Phe Val Arg Asp Trp Phe Arg Ala Cys His 
                85                  90                  95      


Lys Glu Gly Ile Asn Thr Cys Leu Asp Thr Asn Gly Phe Val Arg His 
            100                 105                 110         


His Asp His Ile Ile Asp Glu Leu Ile Asp Asp Thr Asp Leu Val Leu 
        115                 120                 125             


Leu Asp Leu Lys Glu Met Asn Glu Arg Val His Glu Ser Leu Ile Gly 
    130                 135                 140                 


Val Pro Asn Lys Arg Val Leu Glu Phe Ala Lys Tyr Leu Ala Asp Arg 
145                 150                 155                 160 


Asn Gln Arg Thr Trp Ile Arg His Val Val Val Pro Gly Tyr Thr Asp 
                165                 170                 175     


Ser Asp Glu Asp Leu His Met Leu Gly Asn Phe Ile Lys Asp Met Lys 
            180                 185                 190         


Asn Ile Glu Lys Val Glu Leu Leu Pro Tyr His Arg Leu Gly Ala His 
        195                 200                 205             


Lys Trp Glu Val Leu Gly Asp Lys Tyr Glu Leu Glu Asp Val Lys Pro 
    210                 215                 220                 


Pro Thr Lys Glu Leu Met Glu His Val Lys Gly Leu Leu Ala Gly Tyr 
225                 230                 235                 240 


Gly Leu Asn Val Thr Tyr 
                245     


<210>  31
<211>  2313
<212>  DNA
<213>  Basfia succiniciproducens


<220>
<221>  misc_feature
<222>  (1)..(2313)
<223>  nucleotide sequence of pflD-gene from strain DD1

<400>  31
atggctgaat taacagaagc tcaaaaaaaa gcatgggaag gattcgttcc cggtgaatgg     60

caaaacggcg taaatttacg tgactttatc caaaaaaact atactccgta tgaaggtgac    120

gaatcattct tagctgatgc gactcctgca accagcgagt tgtggaacag cgtgatggaa    180

ggcatcaaaa tcgaaaacaa aactcacgca cctttagatt tcgacgaaca tactccgtca    240

actatcactt ctcacaagcc tggttatatc aataaagatt tagaaaaaat cgttggtctt    300

caaacagacg ctccgttaaa acgtgcaatt atgccgtacg gcggtatcaa aatgatcaaa    360

ggttcttgcg aagtttacgg tcgtaaatta gatccgcaag tagaatttat tttcaccgaa    420

tatcgtaaaa cccataacca aggcgtattc gacgtttata cgccggatat tttacgctgc    480

cgtaaatcag gcgtgttaac cggtttaccg gatgcttacg gtcgtggtcg tattatcggt    540

gactaccgtc gtttagcggt atacggtatt gattacctga tgaaagataa aaaagcccaa    600

ttcgattcat tacaaccgcg tttggaagcg ggcgaagaca ttcaggcaac tatccaatta    660

cgtgaagaaa ttgccgaaca acaccgcgct ttaggcaaaa tcaaagaaat ggcggcatct    720

tacggttacg acatttccgg ccctgcgaca aacgcacagg aagcaatcca atggacatat    780

tttgcttatc tggcagcggt taaatcacaa aacggtgcgg caatgtcatt cggtcgtacg    840

tctacattct tagatatcta tatcgaacgt gacttaaaac gcggtttaat cactgaacaa    900

caggcgcagg aattaatgga ccacttagta atgaaattac gtatggttcg tttcttacgt    960

acgccggaat acgatcaatt attctcaggc gacccgatgt gggcaaccga aactatcgcc   1020

ggtatgggct tagacggtcg tccgttggta actaaaaaca gcttccgcgt attacatact   1080

ttatacacta tgggtacttc tccggaacca aacttaacta ttctttggtc cgaacaatta   1140

cctgaagcgt tcaaacgttt ctgtgcgaaa gtatctattg atacttcctc cgtacaatac   1200

gaaaatgatg acttaatgcg tcctgacttc aacaacgatg actatgcaat cgcatgctgc   1260

gtatcaccga tggtcgtagg taaacaaatg caattcttcg gtgcgcgcgc aaacttagct   1320

aaaactatgt tatacgcaat taacggcggt atcgatgaga aaaatggtat gcaagtcggt   1380

cctaaaactg cgccgattac agacgaagta ttgaatttcg ataccgtaat cgaacgtatg   1440

gacagtttca tggactggtt ggcgactcaa tatgtaaccg cattgaacat catccacttc   1500

atgcacgata aatatgcata tgaagcggca ttgatggcgt tccacgatcg cgacgtattc   1560

cgtacaatgg cttgcggtat cgcgggtctt tccgtggctg cggactcatt atccgcaatc   1620

aaatatgcga aagttaaacc gattcgcggc gacatcaaag ataaagacgg taatgtcgtg   1680

gcctcgaatg ttgctatcga cttcgaaatt gaaggcgaat atccgcaatt cggtaacaat   1740

gatccgcgtg ttgatgattt agcggtagac ttagttgaac gtttcatgaa aaaagttcaa   1800

aaacacaaaa cttaccgcaa cgcaactccg acacaatcta tcctgactat cacttctaac   1860

gtggtatacg gtaagaaaac cggtaatact ccggacggtc gtcgagcagg cgcgccattc   1920

ggaccgggtg caaacccaat gcacggtcgt gaccaaaaag gtgcggttgc ttcacttact   1980

tctgtggcta aacttccgtt cgcttacgcg aaagacggta tttcatatac cttctctatc   2040

gtaccgaacg cattaggtaa agatgacgaa gcgcaaaaac gcaaccttgc cggtttaatg   2100

gacggttatt tccatcatga agcgacagtg gaaggcggtc aacacttgaa tgttaacgtt   2160

cttaaccgtg aaatgttgtt agacgcgatg gaaaatccgg aaaaataccc gcaattaacc   2220

attcgtgttt caggttacgc ggttcgtttc aactcattaa ctaaagagca acaacaagac   2280

gtcatcactc gtacgtttac acaatcaatg taa                                2313


<210>  32
<211>  770
<212>  PRT
<213>  Basfia succiniciproducens


<220>
<221>  SITE
<222>  (1)..(770)
<223>  amino acid of PflD from strain DD1

<400>  32

Met Ala Glu Leu Thr Glu Ala Gln Lys Lys Ala Trp Glu Gly Phe Val 
1               5                   10                  15      


Pro Gly Glu Trp Gln Asn Gly Val Asn Leu Arg Asp Phe Ile Gln Lys 
            20                  25                  30          


Asn Tyr Thr Pro Tyr Glu Gly Asp Glu Ser Phe Leu Ala Asp Ala Thr 
        35                  40                  45              


Pro Ala Thr Ser Glu Leu Trp Asn Ser Val Met Glu Gly Ile Lys Ile 
    50                  55                  60                  


Glu Asn Lys Thr His Ala Pro Leu Asp Phe Asp Glu His Thr Pro Ser 
65                  70                  75                  80  


Thr Ile Thr Ser His Lys Pro Gly Tyr Ile Asn Lys Asp Leu Glu Lys 
                85                  90                  95      


Ile Val Gly Leu Gln Thr Asp Ala Pro Leu Lys Arg Ala Ile Met Pro 
            100                 105                 110         


Tyr Gly Gly Ile Lys Met Ile Lys Gly Ser Cys Glu Val Tyr Gly Arg 
        115                 120                 125             


Lys Leu Asp Pro Gln Val Glu Phe Ile Phe Thr Glu Tyr Arg Lys Thr 
    130                 135                 140                 


His Asn Gln Gly Val Phe Asp Val Tyr Thr Pro Asp Ile Leu Arg Cys 
145                 150                 155                 160 


Arg Lys Ser Gly Val Leu Thr Gly Leu Pro Asp Ala Tyr Gly Arg Gly 
                165                 170                 175     


Arg Ile Ile Gly Asp Tyr Arg Arg Leu Ala Val Tyr Gly Ile Asp Tyr 
            180                 185                 190         


Leu Met Lys Asp Lys Lys Ala Gln Phe Asp Ser Leu Gln Pro Arg Leu 
        195                 200                 205             


Glu Ala Gly Glu Asp Ile Gln Ala Thr Ile Gln Leu Arg Glu Glu Ile 
    210                 215                 220                 


Ala Glu Gln His Arg Ala Leu Gly Lys Ile Lys Glu Met Ala Ala Ser 
225                 230                 235                 240 


Tyr Gly Tyr Asp Ile Ser Gly Pro Ala Thr Asn Ala Gln Glu Ala Ile 
                245                 250                 255     


Gln Trp Thr Tyr Phe Ala Tyr Leu Ala Ala Val Lys Ser Gln Asn Gly 
            260                 265                 270         


Ala Ala Met Ser Phe Gly Arg Thr Ser Thr Phe Leu Asp Ile Tyr Ile 
        275                 280                 285             


Glu Arg Asp Leu Lys Arg Gly Leu Ile Thr Glu Gln Gln Ala Gln Glu 
    290                 295                 300                 


Leu Met Asp His Leu Val Met Lys Leu Arg Met Val Arg Phe Leu Arg 
305                 310                 315                 320 


Thr Pro Glu Tyr Asp Gln Leu Phe Ser Gly Asp Pro Met Trp Ala Thr 
                325                 330                 335     


Glu Thr Ile Ala Gly Met Gly Leu Asp Gly Arg Pro Leu Val Thr Lys 
            340                 345                 350         


Asn Ser Phe Arg Val Leu His Thr Leu Tyr Thr Met Gly Thr Ser Pro 
        355                 360                 365             


Glu Pro Asn Leu Thr Ile Leu Trp Ser Glu Gln Leu Pro Glu Ala Phe 
    370                 375                 380                 


Lys Arg Phe Cys Ala Lys Val Ser Ile Asp Thr Ser Ser Val Gln Tyr 
385                 390                 395                 400 


Glu Asn Asp Asp Leu Met Arg Pro Asp Phe Asn Asn Asp Asp Tyr Ala 
                405                 410                 415     


Ile Ala Cys Cys Val Ser Pro Met Val Val Gly Lys Gln Met Gln Phe 
            420                 425                 430         


Phe Gly Ala Arg Ala Asn Leu Ala Lys Thr Met Leu Tyr Ala Ile Asn 
        435                 440                 445             


Gly Gly Ile Asp Glu Lys Asn Gly Met Gln Val Gly Pro Lys Thr Ala 
    450                 455                 460                 


Pro Ile Thr Asp Glu Val Leu Asn Phe Asp Thr Val Ile Glu Arg Met 
465                 470                 475                 480 


Asp Ser Phe Met Asp Trp Leu Ala Thr Gln Tyr Val Thr Ala Leu Asn 
                485                 490                 495     


Ile Ile His Phe Met His Asp Lys Tyr Ala Tyr Glu Ala Ala Leu Met 
            500                 505                 510         


Ala Phe His Asp Arg Asp Val Phe Arg Thr Met Ala Cys Gly Ile Ala 
        515                 520                 525             


Gly Leu Ser Val Ala Ala Asp Ser Leu Ser Ala Ile Lys Tyr Ala Lys 
    530                 535                 540                 


Val Lys Pro Ile Arg Gly Asp Ile Lys Asp Lys Asp Gly Asn Val Val 
545                 550                 555                 560 


Ala Ser Asn Val Ala Ile Asp Phe Glu Ile Glu Gly Glu Tyr Pro Gln 
                565                 570                 575     


Phe Gly Asn Asn Asp Pro Arg Val Asp Asp Leu Ala Val Asp Leu Val 
            580                 585                 590         


Glu Arg Phe Met Lys Lys Val Gln Lys His Lys Thr Tyr Arg Asn Ala 
        595                 600                 605             


Thr Pro Thr Gln Ser Ile Leu Thr Ile Thr Ser Asn Val Val Tyr Gly 
    610                 615                 620                 


Lys Lys Thr Gly Asn Thr Pro Asp Gly Arg Arg Ala Gly Ala Pro Phe 
625                 630                 635                 640 


Gly Pro Gly Ala Asn Pro Met His Gly Arg Asp Gln Lys Gly Ala Val 
                645                 650                 655     


Ala Ser Leu Thr Ser Val Ala Lys Leu Pro Phe Ala Tyr Ala Lys Asp 
            660                 665                 670         


Gly Ile Ser Tyr Thr Phe Ser Ile Val Pro Asn Ala Leu Gly Lys Asp 
        675                 680                 685             


Asp Glu Ala Gln Lys Arg Asn Leu Ala Gly Leu Met Asp Gly Tyr Phe 
    690                 695                 700                 


His His Glu Ala Thr Val Glu Gly Gly Gln His Leu Asn Val Asn Val 
705                 710                 715                 720 


Leu Asn Arg Glu Met Leu Leu Asp Ala Met Glu Asn Pro Glu Lys Tyr 
                725                 730                 735     


Pro Gln Leu Thr Ile Arg Val Ser Gly Tyr Ala Val Arg Phe Asn Ser 
            740                 745                 750         


Leu Thr Lys Glu Gln Gln Gln Asp Val Ile Thr Arg Thr Phe Thr Gln 
        755                 760                 765             


Ser Met 
    770 


