                         SEQUENCE LISTING

<110>  The Johns Hopkins University
 
<120>  AN N-TERMINAL SIGNAL PEPTIDE INCREASES TOTAL EXPRESSION AND 
       ENHANCES SURFACE TRAFFICKING OF PROTEINS ON CELL SURFACES

<130>  P12834-02

<150>  61933022
<151>  2014-01-29

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  147
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence comprising Lucy tag, HA tag, linker and Rho 
       tag

<400>  1
atgagacccc agatcctgct gctcctggcc ctgctgaccc taggcctggc ttacccatac       60

gatgttccag attacgctat cgaattgatg aacgggaccg agggcccaaa cttctacgtg      120

cctttctcca acaagacggg cgtggtg                                          147


<210>  2
<211>  49
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence comprising Lucy tag, HA tag, linker and Rho 
       tag

<400>  2

Met Arg Pro Gln Ile Leu Leu Leu Leu Ala Leu Leu Thr Leu Gly Leu 
1               5                   10                  15      


Ala Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ile Glu Leu Met Asn Gly 
            20                  25                  30          


Thr Glu Gly Pro Asn Phe Tyr Val Pro Phe Ser Asn Lys Thr Gly Val 
        35                  40                  45              


Val 
    


<210>  3
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of Lucy tag

<400>  3

Met Arg Pro Gln Ile Leu Leu Leu Leu Ala Leu Leu Thr Leu Gly Leu 
1               5                   10                  15      


Ala 
    


<210>  4
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the Lucy tag

<400>  4
atgagacccc agatcctgct gctcctggcc ctgctgaccc taggcctggc t                51


<210>  5
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the HA tag

<400>  5
tacccatacg atgttccaga ttacgct                                           27


<210>  6
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the HA tag

<400>  6

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  7
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  9 nucleotide linker

<400>  7
atcgaattg                                                                9


<210>  8
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3 amino acid linker

<400>  8

Ile Glu Leu 
1           


<210>  9
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the Rho tag

<400>  9
atgaacggga ccgagggccc aaacttctac gtgcctttct ccaacaagac gggcgtggtg       60


<210>  10
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the Rho tag

<400>  10

Met Asn Gly Thr Glu Gly Pro Asn Phe Tyr Val Pro Phe Ser Asn Lys 
1               5                   10                  15      


Thr Gly Val Val 
            20  


<210>  11
<211>  153
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding the Lucy tag, HA tag, linker, Rho 
       tag, EcoRI site for cloning

<400>  11
atgagacccc agatcctgct gctcctggcc ctgctgaccc taggcctggc ttacccatac       60

gatgttccag attacgctat cgaattgatg aacgggaccg agggcccaaa cttctacgtg      120

cctttctcca acaagacggg cgtggtggaa ttc                                   153


<210>  12
<211>  51
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the Lucy tag, HA tag, linker, Rho tag, 
       EcoRI site for cloning

<400>  12

Met Arg Pro Gln Ile Leu Leu Leu Leu Ala Leu Leu Thr Leu Gly Leu 
1               5                   10                  15      


Ala Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ile Glu Leu Met Asn Gly 
            20                  25                  30          


Thr Glu Gly Pro Asn Phe Tyr Val Pro Phe Ser Asn Lys Thr Gly Val 
        35                  40                  45              


Val Glu Phe 
    50      


<210>  13
<211>  144
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence comprising Lucy tag, flag tag, linker and Rho
       tag

<400>  13
atgagacccc agatcctgct gctcctggcc ctgctgaccc taggcctggc tgattacaag       60

gacgacgacg ataagatcga attgatgaac gggaccgagg gcccaaactt ctacgtgcct      120

ttctccaaca agacgggcgt ggtg                                             144


<210>  14
<211>  48
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence comprising Lucy tag, flag tag, linker and Rho
       tag

<400>  14

Met Arg Pro Gln Ile Leu Leu Leu Leu Ala Leu Leu Thr Leu Gly Leu 
1               5                   10                  15      


Ala Asp Tyr Lys Asp Asp Asp Asp Lys Ile Glu Leu Met Asn Gly Thr 
            20                  25                  30          


Glu Gly Pro Asn Phe Tyr Val Pro Phe Ser Asn Lys Thr Gly Val Val 
        35                  40                  45              


<210>  15
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the Flag tag

<400>  15
gattacaagg acgacgacga taag                                              24


<210>  16
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the Flag tag

<400>  16

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  17
<211>  150
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding the Lucy tag, Flag tag, linker, Rho 
       tag, EcoRI site for cloning

<400>  17
atgagacccc agatcctgct gctcctggcc ctgctgaccc taggcctggc tgattacaag       60

gacgacgacg ataagatcga attgatgaac gggaccgagg gcccaaactt ctacgtgcct      120

ttctccaaca agacgggcgt ggtggaattc                                       150


<210>  18
<211>  50
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the Lucy tag, Flag tag, linker, Rho tag 
       and EcoRI cloning site

<400>  18

Met Arg Pro Gln Ile Leu Leu Leu Leu Ala Leu Leu Thr Leu Gly Leu 
1               5                   10                  15      


Ala Asp Tyr Lys Asp Asp Asp Asp Lys Ile Glu Leu Met Asn Gly Thr 
            20                  25                  30          


Glu Gly Pro Asn Phe Tyr Val Pro Phe Ser Asn Lys Thr Gly Val Val 
        35                  40                  45              


Glu Phe 
    50  


