                         SEQUENCE LISTING

<110>  Thomas Jefferson University
       Tykocinski, Mark L.
 
<120>  Fusion Proteins for Modulating Regulatory and Effector T Cells

<130>  205961-0005WO1 (00012)

<150>  US 61/759,157
<151>  2013-01-31

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically synthesized

<400>  1
acaacacacg rgaccttagg ag                                                22


<210>  2
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically synthesized

<400>  2
cactcattcc tgttgaagct c                                                 21


<210>  3
<211>  15
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  hinge region of human IgG1

<400>  3

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  4
<211>  8
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  strep-tag

<400>  4

Trp Ser His Pro Gln Phe Glu Lys 
1               5               


<210>  5
<211>  639
<212>  PRT
<213>  Mus musculus


<220>
<221>  misc_feature
<223>  DTA-1 Heavy Chain-human IgG-mouse OX40L

<400>  5

Met Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala Ile Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Ala Glu 
            20                  25                  30          


Pro Gly Arg Ser Leu Arg Leu Ala Cys Thr Ala Ser Gly Phe Thr Phe 
        35                  40                  45              


Gly Asn Tyr Ala Ile Thr Trp Phe Arg Gln Ala Pro Gly Lys Gly Leu 
    50                  55                  60                  


Glu Trp Val Ala Phe Ile Arg Ser Lys Ile Tyr His Ala Ala Ser Asp 
65                  70                  75                  80  


His Ala Ala Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser 
                85                  90                  95      


Asn Gly Val Ala Tyr Leu Gln Met Asn Ser Leu Gln Thr Glu Asp Thr 
            100                 105                 110         


Ala Val Tyr Tyr Cys Ser Arg Ala Ala Leu Tyr Asp Tyr Gly Asp Tyr 
        115                 120                 125             


Gly Asp Phe Asp Tyr Trp Gly His Gly Thr Leu Val Thr Val Ser Ser 
    130                 135                 140                 


Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
145                 150                 155                 160 


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
                165                 170                 175     


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
            180                 185                 190         


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
        195                 200                 205             


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
    210                 215                 220                 


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
225                 230                 235                 240 


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
            260                 265                 270         


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
        275                 280                 285             


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
    290                 295                 300                 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
305                 310                 315                 320 


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
                325                 330                 335     


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
            340                 345                 350         


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
        355                 360                 365             


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
    370                 375                 380                 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
385                 390                 395                 400 


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
                405                 410                 415     


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
            420                 425                 430         


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
        435                 440                 445             


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
    450                 455                 460                 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Glu Pro Lys Ser Cys Asp 
465                 470                 475                 480 


Lys Thr His Thr Cys Pro Pro Cys Pro Gln Leu Ser Ser Ser Pro Ala 
                485                 490                 495     


Lys Asp Pro Pro Ile Gln Arg Leu Arg Gly Ala Val Thr Arg Cys Glu 
            500                 505                 510         


Asp Gly Gln Leu Phe Ile Ser Ser Tyr Lys Asn Glu Tyr Gln Thr Met 
        515                 520                 525             


Glu Val Gln Asn Asn Ser Val Val Ile Lys Cys Asp Gly Leu Tyr Ile 
    530                 535                 540                 


Ile Tyr Leu Lys Gly Ser Phe Phe Gln Glu Val Lys Ile Asp Leu His 
545                 550                 555                 560 


Phe Arg Glu Asp His Asn Pro Ile Ser Ile Pro Met Leu Asn Asp Gly 
                565                 570                 575     


Arg Arg Ile Val Phe Thr Val Val Ala Ser Leu Ala Phe Lys Asp Lys 
            580                 585                 590         


Val Tyr Leu Thr Val Asn Ala Pro Asp Thr Leu Cys Glu His Leu Gln 
        595                 600                 605             


Ile Asn Asp Gly Glu Leu Ile Val Val Gln Leu Thr Pro Gly Tyr Cys 
    610                 615                 620                 


Ala Pro Glu Gly Ser Tyr His Ser Thr Val Asn Gln Val Pro Leu 
625                 630                 635                 


<210>  6
<211>  232
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  DTA-1 Light Chain

<400>  6

Met Gly Leu Ser Trp Val Phe Leu Val Ala Leu Leu Lys Gly Ile Gln 
1               5                   10                  15      


Cys Glu Val Gln Leu Val Glu Thr Gly Gly Gly Leu Val Arg Pro Gly 
            20                  25                  30          


Ser Ser Leu Lys Leu Ser Cys Ala Thr Ser Gly Phe Thr Phe Ser Asn 
        35                  40                  45              


Thr Trp Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp 
    50                  55                  60                  


Val Ala Leu Ile Lys Glu Lys Tyr Thr Asn Tyr Glu Ala Lys Tyr Ala 
65                  70                  75                  80  


Glu Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Ser 
                85                  90                  95      


Arg Val Tyr Leu Gln Met Asn Thr Leu Arg Asp Gln Asp Thr Ala Thr 
            100                 105                 110         


Tyr Tyr Cys Thr Val Gln Leu Gly Pro Phe Asp Tyr Trp Gly Gln Gly 
        115                 120                 125             


Val Thr Val Thr Val Ser Ser Ala Gln Thr Thr Ala Pro Ser Val Tyr 
    130                 135                 140                 


Pro Leu Ala Pro Gly Cys Gly Asp Thr Thr Ser Ser Thr Val Thr Leu 
145                 150                 155                 160 


Gly Cys Leu Val Lys Gly Tyr Phe Pro Glu Pro Val Thr Val Thr Trp 
                165                 170                 175     


Asn Ser Gly Ala Leu Ser Ser Asp Val His Thr Phe Pro Ala Val Leu 
            180                 185                 190         


Gln Ser Gly Leu Tyr Thr Leu Thr Ser Ser Val Thr Ser Ser Thr Trp 
        195                 200                 205             


Pro Ser Gln Thr Val Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr 
    210                 215                 220                 


Lys Val Asp Lys Lys Val Gly Ser 
225                 230         


<210>  7
<211>  158
<212>  PRT
<213>  Mus musculus


<220>
<221>  misc_feature
<223>  STREP-tag-mouse OX40L

<400>  7

Trp Ser His Pro Gln Phe Glu Lys Gln Leu Ser Ser Ser Pro Ala Lys 
1               5                   10                  15      


Asp Pro Pro Ile Gln Arg Leu Arg Gly Ala Val Thr Arg Cys Glu Asp 
            20                  25                  30          


Gly Gln Leu Phe Ile Ser Ser Tyr Lys Asn Glu Tyr Gln Thr Met Glu 
        35                  40                  45              


Val Gln Asn Asn Ser Val Val Ile Lys Cys Asp Gly Leu Tyr Ile Ile 
    50                  55                  60                  


Tyr Leu Lys Gly Ser Phe Phe Gln Glu Val Lys Ile Asp Leu His Phe 
65                  70                  75                  80  


Arg Glu Asp His Asn Pro Ile Ser Ile Pro Met Leu Asn Asp Gly Arg 
                85                  90                  95      


Arg Ile Val Phe Thr Val Val Ala Ser Leu Ala Phe Lys Asp Lys Val 
            100                 105                 110         


Tyr Leu Thr Val Asn Ala Pro Asp Thr Leu Cys Glu His Leu Gln Ile 
        115                 120                 125             


Asn Asp Gly Glu Leu Ile Val Val Gln Leu Thr Pro Gly Tyr Cys Ala 
    130                 135                 140                 


Pro Glu Gly Ser Tyr His Ser Thr Val Asn Gln Val Pro Leu 
145                 150                 155             


