                         SEQUENCE LISTING

<110>  Institut National de la Recherche Agronomique
       Institut Franais de la Vigne et du Vin
       University of Strasbourg
       CNRS
       Vrije Universiteit Brussel
       Vlaams Instituut voor Biotechnologie
 
<120>  RESISTANCE TO GRAPEVINE FANLEAF VIRUS

<130>  B1767

<160>  43    

<170>  PatentIn version 3.3

<210>  1
<211>  131
<212>  PRT
<213>  artificial sequence

<220>
<223>  Nb126

<400>  1

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val Gln Val Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ala Cys Ala Ala Ser Gly Asp Thr Phe Ser Gly Tyr 
            20                  25                  30          


Leu Ala Ala Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Gly Val 
        35                  40                  45              


Ala Ala Ile Asn Ser Val Arg His Thr Thr Ser Tyr Ala Asn Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Lys Asp Asn Ala Asp Asn Met Met Tyr 
65                  70                  75                  80  


Leu Glu Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Ile Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Ala Asp Ala Ile Gly Leu Ala Glu Tyr Trp Ser Thr Pro Thr 
            100                 105                 110         


Leu Ser Ala Ala Arg Tyr Lys Tyr Trp Gly Gln Gly Thr Gln Val Thr 
        115                 120                 125             


Val Ser Ser 
    130     


<210>  2
<211>  131
<212>  PRT
<213>  artificial sequence

<220>
<223>  Nb101

<400>  2

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val Gln Val Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ala Cys Ala Ala Ser Gly Asp Thr Phe Ser Gly Tyr 
            20                  25                  30          


Leu Ala Ala Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Gly Val 
        35                  40                  45              


Ala Ala Ile Asn Ser Val Arg His Thr Thr Ser Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Lys Asp Asn Ala Asp Asn Met Met Tyr 
65                  70                  75                  80  


Leu Glu Met Asn Gly Leu Lys Pro Glu Asp Thr Ala Ile Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Ala Asp Ala Ile Gly Leu Ala Glu Tyr Trp Ser Thr Pro Thr 
            100                 105                 110         


Leu Ser Ala Ala Arg Tyr Lys Tyr Trp Gly Gln Gly Thr Gln Val Thr 
        115                 120                 125             


Val Ser Ser 
    130     


<210>  3
<211>  131
<212>  PRT
<213>  artificial sequence

<220>
<223>  Nb23

<400>  3

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val Gln Val Gly Gly 
1               5                   10                  15      


Ser Leu Arg Val Ala Cys Ala Ala Ser Gly Asp Thr Phe Ser Gly Tyr 
            20                  25                  30          


Leu Ala Ala Trp Phe Arg Gln Ala Pro Gly Lys Gly Arg Glu Gly Val 
        35                  40                  45              


Ala Ala Ile Asn Ser Lys Arg His Thr Thr Ser Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Lys Asp Asn Ala Asp Asn Ile Met Tyr 
65                  70                  75                  80  


Leu Glu Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Ile Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Ala Asp Ala Ile Gly Leu Ala Glu Tyr Trp Ser Thr Pro Thr 
            100                 105                 110         


Leu Ser Ala Ala Arg Tyr Lys Tyr Trp Gly Gln Gly Thr Gln Val Thr 
        115                 120                 125             


Val Ser Ser 
    130     


<210>  4
<211>  131
<212>  PRT
<213>  artificial sequence

<220>
<223>  Nbp75

<400>  4

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Ser Glu Tyr Pro Ser Ser Ser Thr 
            20                  25                  30          


Ala Met Ala Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Gly Val 
        35                  40                  45              


Ala Ala Ile Asn Ser Val Arg His Thr Thr Ser Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Lys Asp Asn Ala Asp Asn Met Met Tyr 
65                  70                  75                  80  


Leu Glu Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Ile Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Ala Asp Ala Ile Gly Leu Ala Glu Tyr Trp Ser Thr Pro Thr 
            100                 105                 110         


Leu Ser Ala Ala Arg Tyr Lys Tyr Trp Gly Gln Gly Thr Gln Val Thr 
        115                 120                 125             


Val Ser Ser 
    130     


<210>  5
<211>  131
<212>  PRT
<213>  artificial sequence

<220>
<223>  Nbp71

<400>  5

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ala Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Glu Ala Ser Gly Asp Val Pro Glu Asn Gly 
            20                  25                  30          


Tyr Met Ala Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Gly Val 
        35                  40                  45              


Ala Ala Ile Asn Ser Val Arg His Thr Thr Ser Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Lys Asp Asn Ala Asp Asn Met Met Tyr 
65                  70                  75                  80  


Leu Glu Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Ile Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Ala Asp Ala Ile Gly Leu Ala Glu Tyr Trp Ser Thr Pro Thr 
            100                 105                 110         


Leu Ser Ala Ala Arg Tyr Lys Tyr Trp Gly Gln Gly Thr Gln Val Thr 
        115                 120                 125             


Val Ser Ser 
    130     


<210>  6
<211>  10
<212>  PRT
<213>  artificial sequence

<220>
<223>  Nb126 CDR1

<400>  6

Gly Asp Thr Phe Ser Gly Tyr Leu Ala Ala 
1               5                   10  


<210>  7
<211>  17
<212>  PRT
<213>  artificial sequence

<220>
<223>  Nb126 CDR2

<400>  7

Ala Ile Asn Ser Val Arg His Thr Thr Ser Tyr Ala Asn Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  8
<211>  22
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR3

<400>  8

Ala Asp Ala Ile Gly Leu Ala Glu Tyr Trp Ser Thr Pro Thr Leu Ser 
1               5                   10                  15      


Ala Ala Arg Tyr Lys Tyr 
            20          


<210>  9
<211>  17
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Nb101 CDR2

<400>  9

Ala Ile Asn Ser Val Arg His Thr Thr Ser Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  10
<211>  17
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Nb23 CDR2

<400>  10

Ala Ile Asn Ser Lys Arg His Thr Thr Ser Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  11
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Nbp75 CDR1

<400>  11

Glu Tyr Pro Ser Ser Ser Thr Ala Met Ala 
1               5                   10  


<210>  12
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Nbp71 CDR1

<400>  12

Gly Asp Val Pro Glu Asn Gly Tyr Met Ala 
1               5                   10  


<210>  13
<211>  17
<212>  PRT
<213>  Artificial sequence

<220>
<223>  consensus amino acid sequence of CDR2


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is a lysine or a valine residue

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  Xaa is an aspartate or an asparagine residue

<400>  13

Ala Ile Asn Ser Xaa Arg His Thr Thr Ser Tyr Ala Xaa Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  14
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  consensus amino acid sequence of CDR1


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is a glycine or a glutamate residue

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa is an aspartate or a tyrosine residue

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is a threonine or a valine or a proline residue

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is a phenylalanine or a proline or a serine residue

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is a serine or a glutamate residue

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa is a glycine or an asparagine or a serine residue

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa is a tyrosine or a glycine or a threonine residue

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa is a leucine or a tyrosine or an alanine residue

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa is an alanine or a methionine residue

<400>  14

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ala 
1               5                   10  


<210>  15
<211>  25
<212>  PRT
<213>  Artificial sequence

<220>
<223>  consensus amino acid sequence of FR1


<220>
<221>  MISC_FEATURE
<222>  (11)..(11)
<223>  Xaa is a serine or an alanine residue

<220>
<221>  MISC_FEATURE
<222>  (14)..(14)
<223>  Xaa is a valine, a proline or an alanine residue

<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  Xaa is an arginine or a lysine residue

<220>
<221>  MISC_FEATURE
<222>  (20)..(20)
<223>  Xaa is a valine or a leucine residue

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa is an alanine or a serine residue

<220>
<221>  MISC_FEATURE
<222>  (23)..(23)
<223>  Xaa is an alanine, a glutamate or a valine residue

<400>  15

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Xaa Val Gln Xaa Gly Gly 
1               5                   10                  15      


Ser Leu Xaa Xaa Xaa Cys Xaa Ala Ser 
            20                  25  


<210>  16
<211>  14
<212>  PRT
<213>  Artificial sequence

<220>
<223>  consensus amino acid sequence of FR2


<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa is a glycine or a glutamate residue

<400>  16

Trp Phe Arg Gln Ala Pro Gly Lys Xaa Arg Glu Gly Val Ala 
1               5                   10                  


<210>  17
<211>  32
<212>  PRT
<213>  Artificial sequence

<220>
<223>  consensus amino acid sequence of FR3


<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Xaa is a isoleucine or a methionine residue

<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  Xaa is a serine or a glycine residue

<400>  17

Arg Phe Thr Ile Ser Lys Asp Asn Ala Asp Asn Xaa Met Tyr Leu Glu 
1               5                   10                  15      


Met Asn Xaa Leu Lys Pro Glu Asp Thr Ala Ile Tyr Tyr Cys Ala Ala 
            20                  25                  30          


<210>  18
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  amino acid sequence of FR4

<400>  18

Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
1               5                   10      


<210>  19
<211>  53
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer #152

<400>  19
ggggacaagt ttgtacaaaa aagcaggctc tatgaaatac ctattgccta cgg            53


<210>  20
<211>  75
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer #197

<400>  20
ggggaccact ttgtacaaga aagctgggtc ttatttttca aattgaggat gagaccagtg     60

atggtgatgg tggtg                                                      75


<210>  21
<211>  60
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer 429

<400>  21
atcttaggtc accgtctcct caggcggcgg tagcggcggc ggtatggtga gcaagggcga     60


<210>  22
<211>  53
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer 430

<400>  22
tactctgaat tctattagtg atgatgatgg tggtgcttgt acagctcgtc cat            53


<210>  23
<211>  50
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer #153

<400>  23
ggggacaagt ttgtacaaaa aagcaggctc tatgcaggtg cagctgcagg                50


<210>  24
<211>  49
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer #150

<400>  24
ggggaccact ttgtacaaga aagctgggtc cttgtacagc tcgtccatg                 49


<210>  25
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer #397

<400>  25
tcaaatcata tgcaggtgca gctgcagg                                        28


<210>  26
<211>  31
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer #399

<400>  26
gtgactcgag ttagtgatgg tgatggtgat g                                    31


<210>  27
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  RNA1-fwd primer

<400>  27
cccaaaagtc atcgcaatgc t                                               21


<210>  28
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  RNA1-rev primer

<400>  28
ggatcaggat atggaaagca c                                               21


<210>  29
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  EGFP-fwd primer

<400>  29
tatatcatgg ccgacaagca                                                 20


<210>  30
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  EGFP-rev primer

<400>  30
gaactccagc aggaccatgt                                                 20


<210>  31
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  P19-fwd primer

<400>  31
tgatacatta ctttccactt cgat                                            24


<210>  32
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  P19-rev primer

<400>  32
tgagatggca attcggtcta                                                 20


<210>  33
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CDC2-fwd primer

<400>  33
gtgtagtgta caaggctcgt ga                                              22


<210>  34
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CDC2-rev primer

<400>  34
tagctgtgct tggtactccc tc                                              22


<210>  35
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  EF1a-fwd primer

<400>  35
gtactgtccc tgttggtcgt                                                 20


<210>  36
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  EF1a-rev primer

<400>  36
gtaggtccaa aggtcacaac cat                                             23


<210>  37
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  ACT-fwd primer

<400>  37
aaattactgc acttgctcct agc                                             23


<210>  38
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  ACT-rev primer

<400>  38
caatccagac actgtatttc ctctc                                           25


<210>  39
<211>  393
<212>  DNA
<213>  Artificial

<220>
<223>  Nb23 coding sequence

<400>  39
caggtgcagc tgcaggagtc tgggggaggc tcggtgcagg tcggagggtc tctgagagtc     60

gcctgtgcag cctctggaga caccttcagt ggctacttag cggcctggtt ccgccaggct    120

ccagggaagg ggcgtgaggg ggtcgcagcc attaatagta agcgtcatac cacatcgtac    180

gccgactccg tgaagggccg attcaccatc tccaaggaca acgccgacaa tatcatgtat    240

ctggaaatga acagcctgaa acctgaggac acggccatct attactgtgc ggcggccgac    300

gccattgggt tagctgaata ctggtcaact cctacccttt ccgccgcgag atataagtac    360

tggggccagg ggacccaggt caccgtctcc tca                                 393


<210>  40
<211>  393
<212>  DNA
<213>  Artificial

<220>
<223>  Nb101 coding sequence

<400>  40
caggtgcagc tgcaggagtc tgggggaggc tcggtgcagg ttggagggtc tctgagactc     60

gcctgtgcag cctctggaga caccttcagt ggctacttag cggcctggtt ccgccaggct    120

ccagggaagg agcgtgaggg ggtcgcagcc attaatagtg ttcgtcatac cacatcgtac    180

gccgactccg tgaagggccg attcaccatc tccaaggaca acgccgacaa tatgatgtat    240

ctggaaatga acggcctgaa acctgaggac acggccatct attactgtgc ggcggccgac    300

gccattgggt tagctgaata ctggtcaact cctacccttt ccgccgcgag gtataagtac    360

tggggccagg ggacccaggt caccgtctcc tca                                 393


<210>  41
<211>  393
<212>  DNA
<213>  Artificial

<220>
<223>  Nb126 coding sequence

<400>  41
caggtgcagc tgcaggagtc tgggggaggc tcggtgcagg ttggagggtc tctgagactc     60

gcctgtgcgg cctctggaga caccttcagt ggctacttag cggcctggtt ccgccaggct    120

ccagggaagg agcgtgaggg ggtcgcagcc attaatagtg ttcgtcatac cacatcgtac    180

gccaactccg tgaagggccg attcaccatc tccaaggaca acgccgacaa tatgatgtat    240

ttggaaatga acagcctgaa acctgaggac acggccatct attactgtgc ggcggccgac    300

gccattgggt tagctgaata ctggtcaact cctacccttt ccgccgcgag gtataagtac    360

tggggccagg ggacccaggt caccgtctcc agc                                 393


<210>  42
<211>  393
<212>  DNA
<213>  Artificial

<220>
<223>  Nb71 coding sequence

<400>  42
caggtgcagc tgcaggagtc tgggggagga gcggtgcagc ctggagggtc cctaaagctc     60

tcctgtgaag cttctggaga cgttcccgag aatggctaca tggcttggtt ccgccaggct    120

ccagggaaag agcgtgaggg ggtcgcagcc attaatagtg ttcgtcatac cacatcgtac    180

gccgactccg tgaagggccg attcaccatc tccaaggaca acgccgacaa tatgatgtat    240

ctggaaatga acagcctgaa acctgaggac acggccatct attactgtgc ggcggccgac    300

gccattgggt tagctgaata ctggtcaact cctacccttt ccgccgcgag gtataagtac    360

tggggccagg ggacccaggt caccgtctcc tca                                 393


<210>  43
<211>  393
<212>  DNA
<213>  Artificial

<220>
<223>  Nb75 coding sequence

<400>  43
caggtgcagc tgcaggagtc tgggggaggc tcggtgcagg ctggaggctc tctgagactc     60

tcctgtgtag cctctgaata ccccagtagt agcaccgcga tggcttggtt ccgccaggct    120

ccagggaagg agcgtgaggg ggtcgcagcc attaatagtg ttcgtcatac cacatcgtac    180

gccgactccg tgaagggccg attcaccatc tccaaggaca acgccgacaa tatgatgtat    240

ctggaaatga acagcctgaa acctgaggac acggccatct attactgtgc ggcggccgac    300

gccattgggt tagctgaata ctggtcaact cctacccttt ccgccgcgag gtataagtac    360

tggggccagg ggacccaggt caccgtctcc tca                                 393


