                         SEQUENCE LISTING

<110>  Novozymes A/S
       Lavigne, James
       Kolczynski, Daniel
       Scott, Brian R.
 
<120>  Modified Family 5 Glycosyl Hydrolases with Higher Thermostability

<130>  12759-WO-PCT

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  1436
<212>  DNA
<213>  Trichoderma reesei

<400>  1
atgaacaagt ccgtggctcc attgctgctt gcagcgtcca tactatatgg cggcgccgct       60

gcacagcaga ctgtctgggg ccagtgtgga ggtattggtt ggagcggacc tacgaattgt      120

gctcctggct cagcttgttc gaccctcaat ccttattatg cgcaatgtat tccgggagcc      180

actactatca ccacttcgac ccggccacca tccggtccaa ccaccaccac cagggctacc      240

tcaacaagct catcaactcc acccacgagc tctggggtcc gatttgccgg cgttaacatc      300

gcgggttttg actttggctg taccacagag tgagtaccct tgtttcctgg tgttgctggc      360

tgaaaagttg ggcgggtata cagcgatgcg gactgcaaga acaccgccgg tccgccacca      420

tcaagatgtg ggtggtaagc ggcggtgttt tgtacaacta cctgacagct cactcaggaa      480

ctgagaatta atggaagtct tgttacagtg gcacttgcgt tacctcgaag gtttatcctc      540

cgttgaagaa cttcaccggc tcaaacaact accccgatgg catcggccag atgcagcact      600

tcgtcaacga cgacgggatg actattttcc gcttacctgt cggatggcag tacctcgtca      660

acaacaattt gggcggcaat cttgattcca cgagcatttc caagtatgat cagcttgttc      720

aggggtgcct gtctctgggc gcatactgca tcgtcgacat ccacaattat gctcgatgga      780

acggtgggat cattggtcag ggcggcccta ctaatgctca attcacgagc ctttggtcgc      840

agttggcatc aaagtacgca tctcagtcga gggtgtggtt cggcatcatg aatgagcccc      900

acgacgtgaa catcaacacc tgggctgcca cggtccaaga ggttgtaacc gcaatccgca      960

acgctggtgc tacgtcgcaa ttcatctctt tgcctggaaa tgattggcaa tctgctgggg     1020

ctttcatatc cgatggcagt gcagccgccc tgtctcaagt cacgaacccg gatgggtcaa     1080

caacgaatct gatttttgac gtgcacaaat acttggactc agacaactcc ggtactcacg     1140

ccgaatgtac tacaaataac attgacggcg ccttttctcc gcttgccact tggctccgac     1200

agaacaatcg ccaggctatc ctgacagaaa ccggtggtgg caacgttcag tcctgcatac     1260

aagacatgtg ccagcaaatc caatatctca accagaactc agatgtctat cttggctatg     1320

ttggttgggg tgccggatca tttgatagca cgtatgtcct gacggaaaca ccgactggca     1380

gtggtaactc atggacggac acatccttgg tcagctcgtg tctcgcaaga aagtag         1436


<210>  2
<211>  397
<212>  PRT
<213>  Trichoderma reesei

<400>  2

Gln Gln Thr Val Trp Gly Gln Cys Gly Gly Ile Gly Trp Ser Gly Pro 
1               5                   10                  15      


Thr Asn Cys Ala Pro Gly Ser Ala Cys Ser Thr Leu Asn Pro Tyr Tyr 
            20                  25                  30          


Ala Gln Cys Ile Pro Gly Ala Thr Thr Ile Thr Thr Ser Thr Arg Pro 
        35                  40                  45              


Pro Ser Gly Pro Thr Thr Thr Thr Arg Ala Thr Ser Thr Ser Ser Ser 
    50                  55                  60                  


Thr Pro Pro Thr Ser Ser Gly Val Arg Phe Ala Gly Val Asn Ile Ala 
65                  70                  75                  80  


Gly Phe Asp Phe Gly Cys Thr Thr Asp Gly Thr Cys Val Thr Ser Lys 
                85                  90                  95      


Val Tyr Pro Pro Leu Lys Asn Phe Thr Gly Ser Asn Asn Tyr Pro Asp 
            100                 105                 110         


Gly Ile Gly Gln Met Gln His Phe Val Asn Asp Asp Gly Met Thr Ile 
        115                 120                 125             


Phe Arg Leu Pro Val Gly Trp Gln Tyr Leu Val Asn Asn Asn Leu Gly 
    130                 135                 140                 


Gly Asn Leu Asp Ser Thr Ser Ile Ser Lys Tyr Asp Gln Leu Val Gln 
145                 150                 155                 160 


Gly Cys Leu Ser Leu Gly Ala Tyr Cys Ile Val Asp Ile His Asn Tyr 
                165                 170                 175     


Ala Arg Trp Asn Gly Gly Ile Ile Gly Gln Gly Gly Pro Thr Asn Ala 
            180                 185                 190         


Gln Phe Thr Ser Leu Trp Ser Gln Leu Ala Ser Lys Tyr Ala Ser Gln 
        195                 200                 205             


Ser Arg Val Trp Phe Gly Ile Met Asn Glu Pro His Asp Val Asn Ile 
    210                 215                 220                 


Asn Thr Trp Ala Ala Thr Val Gln Glu Val Val Thr Ala Ile Arg Asn 
225                 230                 235                 240 


Ala Gly Ala Thr Ser Gln Phe Ile Ser Leu Pro Gly Asn Asp Trp Gln 
                245                 250                 255     


Ser Ala Gly Ala Phe Ile Ser Asp Gly Ser Ala Ala Ala Leu Ser Gln 
            260                 265                 270         


Val Thr Asn Pro Asp Gly Ser Thr Thr Asn Leu Ile Phe Asp Val His 
        275                 280                 285             


Lys Tyr Leu Asp Ser Asp Asn Ser Gly Thr His Ala Glu Cys Thr Thr 
    290                 295                 300                 


Asn Asn Ile Asp Gly Ala Phe Ser Pro Leu Ala Thr Trp Leu Arg Gln 
305                 310                 315                 320 


Asn Asn Arg Gln Ala Ile Leu Thr Glu Thr Gly Gly Gly Asn Val Gln 
                325                 330                 335     


Ser Cys Ile Gln Asp Met Cys Gln Gln Ile Gln Tyr Leu Asn Gln Asn 
            340                 345                 350         


Ser Asp Val Tyr Leu Gly Tyr Val Gly Trp Gly Ala Gly Ser Phe Asp 
        355                 360                 365             


Ser Thr Tyr Val Leu Thr Glu Thr Pro Thr Gly Ser Gly Asn Ser Trp 
    370                 375                 380                 


Thr Asp Thr Ser Leu Val Ser Ser Cys Leu Ala Arg Lys 
385                 390                 395         


<210>  3
<211>  418
<212>  PRT
<213>  Trichoderma reesei

<400>  3

Met Asn Lys Ser Val Ala Pro Leu Leu Leu Ala Ala Ser Ile Leu Tyr 
1               5                   10                  15      


Gly Gly Ala Ala Ala Gln Gln Thr Val Trp Gly Gln Cys Gly Gly Ile 
            20                  25                  30          


Gly Trp Ser Gly Pro Thr Asn Cys Ala Pro Gly Ser Ala Cys Ser Thr 
        35                  40                  45              


Leu Asn Pro Tyr Tyr Ala Gln Cys Ile Pro Gly Ala Thr Thr Ile Thr 
    50                  55                  60                  


Thr Ser Thr Arg Pro Pro Ser Gly Pro Thr Thr Thr Thr Arg Ala Thr 
65                  70                  75                  80  


Ser Thr Ser Ser Ser Thr Pro Pro Thr Ser Ser Gly Val Arg Phe Ala 
                85                  90                  95      


Gly Val Asn Ile Ala Gly Phe Asp Phe Gly Cys Thr Thr Asp Gly Thr 
            100                 105                 110         


Cys Val Thr Ser Lys Val Tyr Pro Pro Leu Lys Asn Phe Thr Gly Ser 
        115                 120                 125             


Asn Asn Tyr Pro Asp Gly Ile Gly Gln Met Gln His Phe Val Asn Asp 
    130                 135                 140                 


Asp Gly Met Thr Ile Phe Arg Leu Pro Val Gly Trp Gln Tyr Leu Val 
145                 150                 155                 160 


Asn Asn Asn Leu Gly Gly Asn Leu Asp Ser Thr Ser Ile Ser Lys Tyr 
                165                 170                 175     


Asp Gln Leu Val Gln Gly Cys Leu Ser Leu Gly Ala Tyr Cys Ile Val 
            180                 185                 190         


Asp Ile His Asn Tyr Ala Arg Trp Asn Gly Gly Ile Ile Gly Gln Gly 
        195                 200                 205             


Gly Pro Thr Asn Ala Gln Phe Thr Ser Leu Trp Ser Gln Leu Ala Ser 
    210                 215                 220                 


Lys Tyr Ala Ser Gln Ser Arg Val Trp Phe Gly Ile Met Asn Glu Pro 
225                 230                 235                 240 


His Asp Val Asn Ile Asn Thr Trp Ala Ala Thr Val Gln Glu Val Val 
                245                 250                 255     


Thr Ala Ile Arg Asn Ala Gly Ala Thr Ser Gln Phe Ile Ser Leu Pro 
            260                 265                 270         


Gly Asn Asp Trp Gln Ser Ala Gly Ala Phe Ile Ser Asp Gly Ser Ala 
        275                 280                 285             


Ala Ala Leu Ser Gln Val Thr Asn Pro Asp Gly Ser Thr Thr Asn Leu 
    290                 295                 300                 


Ile Phe Asp Val His Lys Tyr Leu Asp Ser Asp Asn Ser Gly Thr His 
305                 310                 315                 320 


Ala Glu Cys Thr Thr Asn Asn Ile Asp Gly Ala Phe Ser Pro Leu Ala 
                325                 330                 335     


Thr Trp Leu Arg Gln Asn Asn Arg Gln Ala Ile Leu Thr Glu Thr Gly 
            340                 345                 350         


Gly Gly Asn Val Gln Ser Cys Ile Gln Asp Met Cys Gln Gln Ile Gln 
        355                 360                 365             


Tyr Leu Asn Gln Asn Ser Asp Val Tyr Leu Gly Tyr Val Gly Trp Gly 
    370                 375                 380                 


Ala Gly Ser Phe Asp Ser Thr Tyr Val Leu Thr Glu Thr Pro Thr Gly 
385                 390                 395                 400 


Ser Gly Asn Ser Trp Thr Asp Thr Ser Leu Val Ser Ser Cys Leu Ala 
                405                 410                 415     


Arg Lys 
        


<210>  4
<211>  31
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  4
gatcgtcgac atggtctcct tcacctccct c                                      31


<210>  5
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  5
gcaacacctg gcaattcctt acc                                               23


<210>  6
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  6
tactatcacc acttcgaccc ggcc                                              24


<210>  7
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  7
tggctgtgga gaagcgc                                                      17


<210>  8
<211>  32
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  8
ggcacttgcg acacctcgaa gatttatcct cc                                     32


<210>  9
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  9
cgaggtgtcg caagtgccat ctgtggta                                          28


<210>  10
<211>  34
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  10
gctacgtcgc acttcatctc tttgcctgga aatg                                   34


<210>  11
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  11
gagatgaagt gcgacgtagc accagc                                            26


<210>  12
<211>  29
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  12
cttggactca aacaactccg gtactcacg                                         29


<210>  13
<211>  32
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  13
ccggagttgt ttgagtccaa gtatttgtgc ac                                     32


<210>  14
<211>  33
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  14
catgtgccgc caaatccaat atctcaacca gaa                                    33


<210>  15
<211>  32
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  15
attggatttg gcggcacatg ttttgtatgc ag                                     32


