               SEQUENCE LISTING

<110> DSM IP Assets B.V.

<120> Improved enzyme variants

<130> 30115-WO-PCT

<150>  EP14151124.6 
<151>  2014-01-14

<150>  EP14169816.7
<151>  2014-05-26 

<160> 2

<170> BiSSAP 1.2

<210> 1
<211> 3103
<212> DNA
<213> Kluyveromyces lactis

<220> 
<221> source
<222> 1..3103
<223> /organism="Kluyveromyces lactis"
      /mol_type="unassigned DNA"

<400> 1
gaagaccaca tgtcttgcct tattcctgag aatttaagga accccaaaaa ggttcacgaa      60

aatagattgc ctactagggc ttactactat gatcaggata ttttcgaatc tctcaatggg     120

ccttgggctt ttgcgttgtt tgatgcacct cttgacgctc cggatgctaa gaatttagac     180

tgggaaacgg caaagaaatg gagcaccatt tctgtgccat cccattggga acttcaggaa     240

gattggaagt acggtaaacc aatttacacg aacgtacagt accctatccc aatcgacatc     300

ccaaatcctc ccactgtaaa tcctactggt gtttatgcta gaacttttga attagattcg     360

aaatcgattg agtcgttcga gcacagattg agatttgagg gtgtggacaa ttgttacgag     420

ctttatgtta atggtcaata tgtgggtttc aataaggggt cccgtaacgg ggctgaattt     480

gatatccaaa agtacgtttc tgagggcgaa aacttagtgg tcgtcaaggt tttcaagtgg     540

tccgattcca cttatatcga ggaccaagat caatggtggc tctctggtat ttacagagac     600

gtttctttac taaaattgcc taagaaggcc catattgaag atgttagggt cactacaact     660

tttgtggact ctcagtatca ggatgcagag ctttctgtga aagttgatgt ccagggttct     720

tcttatgatc acatcaattt cacactttac gaacctgaag atggatctaa agtttacgat     780

gcaagctctt tgttgaacga ggagaatggg aacacgactt tttcaactaa agaatttatt     840

tccttctcca ccaaaaagaa cgaagaaaca gctttcaaga tcaacgtcaa ggccccagaa     900

cattggaccg cagaaaatcc tactttgtac aagtaccagt tggatttaat tggatctgat     960

ggcagtgtga ttcaatctat taagcaccat gttggtttca gacaagtgga gttgaaggac    1020

ggtaacatta ctgttaatgg caaagacatt ctctttagag gtgtcaacag acatgatcac    1080

catccaaggt tcggtagagc tgtgccatta gattttgttg ttagggactt gattctaatg    1140

aagaagttta acatcaatgc tgttcgtaac tcgcattatc caaaccatcc taaggtgtat    1200

gacctcttcg ataagctggg cttctgggtc attgacgagg cagatcttga aactcatggt    1260

gttcaagagc catttaatcg tcatacgaac ttggaggctg aatatccaga tactaaaaat    1320

aaactctacg atgttaatgc ccattactta tcagataatc cagagtacga ggtcgcgtac    1380

ttagacagag cttcccaact tgtcctaaga gatgtcaatc atccttcgat tattatctgg    1440

tccttgggta acgaagcttg ttatggcaga aaccacaaag ccatgtacaa gttaattaaa    1500

caattggatc ctaccagact tgtgcattat gagggtgact tgaacgcttt gagtgcagat    1560

atctttagtt tcatgtaccc aacatttgaa attatggaaa ggtggaggaa gaaccacact    1620

gatgaaaatg gtaagtttga aaagcctttg atcttgtgtg agtacggcca tgcaatgggt    1680

aacggtcctg gctctttgaa agaatatcaa gagttgttct acaaggagaa gttttaccaa    1740

ggtggcttta tctgggaatg ggcaaatcac ggtattgaat tcgaagatgt tagtactgca    1800

gatggtaagt tgcataaagc ttatgcttat ggtggtgact ttaaggaaga ggttcatgac    1860

ggagtgttca tcatggatgg tttgtgtaac agtgagcata atcctactcc gggccttgta    1920

gagtataaga aggttattga acccgttcat attaaaattg cgcacggatc tgtaacaatc    1980

acaaataagc acgacttcat tacgacagac cacttattgt ttatcgacaa ggacacggga    2040

aagacaatcg acgttccatc tttaaagcca gaagaatctg ttactattcc ttctgataca    2100

acttatgttg ttgccgtgtt gaaagatgat gctggtgttc taaaggcagg tcatgaaatt    2160

gcctggggcc aagctgaact tccattgaag gtacccgatt ttgttacaga gacagcagaa    2220

aaagctgcga agatcaacga cggtaaacgt tatgtctcag ttgaatccag tggattgcat    2280

tttatcttgg acaaattgtt gggtaaaatt gaaagcctaa aggtcaaggg taaggaaatt    2340

tccagcaagt ttgagggttc ttcaatcact ttctggagac ctccaacgaa taatgatgaa    2400

cctagggact ttaagaactg gaagaagtac aatattgatt taatgaagca aaacatccat    2460

ggagtgagtg tcgaaaaagg ttctaatggt tctctagctg tagtcacggt taactctcgt    2520

atatccccag ttgtatttta ctatgggttt gagactgttc agaagtacac gatctttgct    2580

aacaaaataa acttgaacac ttctatgaag cttactggcg aatatcagcc tcctgatttc    2640

ccaagagttg ggtacgaatt ctggctagga gatagttatg aatcatttga atggttaggt    2700

cgcgggcccg gcgaatcata tccggataag aaggaatctc aaagattcgg tctttacgat    2760

tccaaagatg tagaggaatt cgtatatgac tatcctcaag aaaatggaaa tcatacagat    2820

acccactttt tgaacatcaa atttgaaggt gcaggaaaac tatcgatctt ccaaaaggag    2880

aagccattta acttcaagat ttcagacgaa tacggggttg atgaagctgc ccacgcttgt    2940

gacgttaaaa gatacggcag acactatcta aggttggacc atgcaatcca tggtgttggt    3000

agcgaagcat gcggacctgc tgttctggac cagtacagat tgaaagctca agatttcaac    3060

tttgagtttg atctcgcttt tgaataaagg aagcttagtc ttc                      3103


<210> 2
<211> 1025
<212> PRT
<213> Kluyveromyces lactis


<400> 2
Met Ser Cys Leu Ile Pro Glu Asn Leu Arg Asn Pro Lys Lys Val His 
1               5                   10                  15      
Glu Asn Arg Leu Pro Thr Arg Ala Tyr Tyr Tyr Asp Gln Asp Ile Phe 
            20                  25                  30          
Glu Ser Leu Asn Gly Pro Trp Ala Phe Ala Leu Phe Asp Ala Pro Leu 
        35                  40                  45              
Asp Ala Pro Asp Ala Lys Asn Leu Asp Trp Glu Thr Ala Lys Lys Trp 
    50                  55                  60                  
Ser Thr Ile Ser Val Pro Ser His Trp Glu Leu Gln Glu Asp Trp Lys 
65                  70                  75                  80  
Tyr Gly Lys Pro Ile Tyr Thr Asn Val Gln Tyr Pro Ile Pro Ile Asp 
                85                  90                  95      
Ile Pro Asn Pro Pro Thr Val Asn Pro Thr Gly Val Tyr Ala Arg Thr 
            100                 105                 110         
Phe Glu Leu Asp Ser Lys Ser Ile Glu Ser Phe Glu His Arg Leu Arg 
        115                 120                 125             
Phe Glu Gly Val Asp Asn Cys Tyr Glu Leu Tyr Val Asn Gly Gln Tyr 
    130                 135                 140                 
Val Gly Phe Asn Lys Gly Ser Arg Asn Gly Ala Glu Phe Asp Ile Gln 
145                 150                 155                 160 
Lys Tyr Val Ser Glu Gly Glu Asn Leu Val Val Val Lys Val Phe Lys 
                165                 170                 175     
Trp Ser Asp Ser Thr Tyr Ile Glu Asp Gln Asp Gln Trp Trp Leu Ser 
            180                 185                 190         
Gly Ile Tyr Arg Asp Val Ser Leu Leu Lys Leu Pro Lys Lys Ala His 
        195                 200                 205             
Ile Glu Asp Val Arg Val Thr Thr Thr Phe Val Asp Ser Gln Tyr Gln 
    210                 215                 220                 
Asp Ala Glu Leu Ser Val Lys Val Asp Val Gln Gly Ser Ser Tyr Asp 
225                 230                 235                 240 
His Ile Asn Phe Thr Leu Tyr Glu Pro Glu Asp Gly Ser Lys Val Tyr 
                245                 250                 255     
Asp Ala Ser Ser Leu Leu Asn Glu Glu Asn Gly Asn Thr Thr Phe Ser 
            260                 265                 270         
Thr Lys Glu Phe Ile Ser Phe Ser Thr Lys Lys Asn Glu Glu Thr Ala 
        275                 280                 285             
Phe Lys Ile Asn Val Lys Ala Pro Glu His Trp Thr Ala Glu Asn Pro 
    290                 295                 300                 
Thr Leu Tyr Lys Tyr Gln Leu Asp Leu Ile Gly Ser Asp Gly Ser Val 
305                 310                 315                 320 
Ile Gln Ser Ile Lys His His Val Gly Phe Arg Gln Val Glu Leu Lys 
                325                 330                 335     
Asp Gly Asn Ile Thr Val Asn Gly Lys Asp Ile Leu Phe Arg Gly Val 
            340                 345                 350         
Asn Arg His Asp His His Pro Arg Phe Gly Arg Ala Val Pro Leu Asp 
        355                 360                 365             
Phe Val Val Arg Asp Leu Ile Leu Met Lys Lys Phe Asn Ile Asn Ala 
    370                 375                 380                 
Val Arg Asn Ser His Tyr Pro Asn His Pro Lys Val Tyr Asp Leu Phe 
385                 390                 395                 400 
Asp Lys Leu Gly Phe Trp Val Ile Asp Glu Ala Asp Leu Glu Thr His 
                405                 410                 415     
Gly Val Gln Glu Pro Phe Asn Arg His Thr Asn Leu Glu Ala Glu Tyr 
            420                 425                 430         
Pro Asp Thr Lys Asn Lys Leu Tyr Asp Val Asn Ala His Tyr Leu Ser 
        435                 440                 445             
Asp Asn Pro Glu Tyr Glu Val Ala Tyr Leu Asp Arg Ala Ser Gln Leu 
    450                 455                 460                 
Val Leu Arg Asp Val Asn His Pro Ser Ile Ile Ile Trp Ser Leu Gly 
465                 470                 475                 480 
Asn Glu Ala Cys Tyr Gly Arg Asn His Lys Ala Met Tyr Lys Leu Ile 
                485                 490                 495     
Lys Gln Leu Asp Pro Thr Arg Leu Val His Tyr Glu Gly Asp Leu Asn 
            500                 505                 510         
Ala Leu Ser Ala Asp Ile Phe Ser Phe Met Tyr Pro Thr Phe Glu Ile 
        515                 520                 525             
Met Glu Arg Trp Arg Lys Asn His Thr Asp Glu Asn Gly Lys Phe Glu 
    530                 535                 540                 
Lys Pro Leu Ile Leu Cys Glu Tyr Gly His Ala Met Gly Asn Gly Pro 
545                 550                 555                 560 
Gly Ser Leu Lys Glu Tyr Gln Glu Leu Phe Tyr Lys Glu Lys Phe Tyr 
                565                 570                 575     
Gln Gly Gly Phe Ile Trp Glu Trp Ala Asn His Gly Ile Glu Phe Glu 
            580                 585                 590         
Asp Val Ser Thr Ala Asp Gly Lys Leu His Lys Ala Tyr Ala Tyr Gly 
        595                 600                 605             
Gly Asp Phe Lys Glu Glu Val His Asp Gly Val Phe Ile Met Asp Gly 
    610                 615                 620                 
Leu Cys Asn Ser Glu His Asn Pro Thr Pro Gly Leu Val Glu Tyr Lys 
625                 630                 635                 640 
Lys Val Ile Glu Pro Val His Ile Lys Ile Ala His Gly Ser Val Thr 
                645                 650                 655     
Ile Thr Asn Lys His Asp Phe Ile Thr Thr Asp His Leu Leu Phe Ile 
            660                 665                 670         
Asp Lys Asp Thr Gly Lys Thr Ile Asp Val Pro Ser Leu Lys Pro Glu 
        675                 680                 685             
Glu Ser Val Thr Ile Pro Ser Asp Thr Thr Tyr Val Val Ala Val Leu 
    690                 695                 700                 
Lys Asp Asp Ala Gly Val Leu Lys Ala Gly His Glu Ile Ala Trp Gly 
705                 710                 715                 720 
Gln Ala Glu Leu Pro Leu Lys Val Pro Asp Phe Val Thr Glu Thr Ala 
                725                 730                 735     
Glu Lys Ala Ala Lys Ile Asn Asp Gly Lys Arg Tyr Val Ser Val Glu 
            740                 745                 750         
Ser Ser Gly Leu His Phe Ile Leu Asp Lys Leu Leu Gly Lys Ile Glu 
        755                 760                 765             
Ser Leu Lys Val Lys Gly Lys Glu Ile Ser Ser Lys Phe Glu Gly Ser 
    770                 775                 780                 
Ser Ile Thr Phe Trp Arg Pro Pro Thr Asn Asn Asp Glu Pro Arg Asp 
785                 790                 795                 800 
Phe Lys Asn Trp Lys Lys Tyr Asn Ile Asp Leu Met Lys Gln Asn Ile 
                805                 810                 815     
His Gly Val Ser Val Glu Lys Gly Ser Asn Gly Ser Leu Ala Val Val 
            820                 825                 830         
Thr Val Asn Ser Arg Ile Ser Pro Val Val Phe Tyr Tyr Gly Phe Glu 
        835                 840                 845             
Thr Val Gln Lys Tyr Thr Ile Phe Ala Asn Lys Ile Asn Leu Asn Thr 
    850                 855                 860                 
Ser Met Lys Leu Thr Gly Glu Tyr Gln Pro Pro Asp Phe Pro Arg Val 
865                 870                 875                 880 
Gly Tyr Glu Phe Trp Leu Gly Asp Ser Tyr Glu Ser Phe Glu Trp Leu 
                885                 890                 895     
Gly Arg Gly Pro Gly Glu Ser Tyr Pro Asp Lys Lys Glu Ser Gln Arg 
            900                 905                 910         
Phe Gly Leu Tyr Asp Ser Lys Asp Val Glu Glu Phe Val Tyr Asp Tyr 
        915                 920                 925             
Pro Gln Glu Asn Gly Asn His Thr Asp Thr His Phe Leu Asn Ile Lys 
    930                 935                 940                 
Phe Glu Gly Ala Gly Lys Leu Ser Ile Phe Gln Lys Glu Lys Pro Phe 
945                 950                 955                 960 
Asn Phe Lys Ile Ser Asp Glu Tyr Gly Val Asp Glu Ala Ala His Ala 
                965                 970                 975     
Cys Asp Val Lys Arg Tyr Gly Arg His Tyr Leu Arg Leu Asp His Ala 
            980                 985                 990         
Ile His Gly Val Gly Ser Glu Ala Cys Gly Pro Ala Val Leu Asp Gln 
        995                 1000                1005            
Tyr Arg Leu Lys Ala Gln Asp Phe Asn Phe Glu Phe Asp Leu Ala Phe 
    1010                1015                1020                
Glu 
1025

