                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  YIELD IMPROVEMENT BY PH-STABILIZATION

<130>  NZ 12630-WO-PCT

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  2586
<212>  DNA
<213>  Bacillus acidopullulyticus


<220>
<221>  CDS
<222>  (1)..(2583)

<400>  1
atg tcc cta ata cgt tct agg tat aat cat ttt gtc att ctt ttt act         48
Met Ser Leu Ile Arg Ser Arg Tyr Asn His Phe Val Ile Leu Phe Thr           
1               5                   10                  15                

gtc gcc ata atg ttt cta aca gtt tgt ttc ccc gct tat aaa gct tta         96
Val Ala Ile Met Phe Leu Thr Val Cys Phe Pro Ala Tyr Lys Ala Leu           
            20                  25                  30                    

gca gat tct acc tcg aca gaa gtc att gtg cat tat cat cgt ttt gat        144
Ala Asp Ser Thr Ser Thr Glu Val Ile Val His Tyr His Arg Phe Asp           
        35                  40                  45                        

tct aac tat gca aat tgg gat cta tgg atg tgg cca tat caa cca gtt        192
Ser Asn Tyr Ala Asn Trp Asp Leu Trp Met Trp Pro Tyr Gln Pro Val           
    50                  55                  60                            

aat ggt aat gga gca gca tac gag ttt tct gga aag gat gat ttt ggc        240
Asn Gly Asn Gly Ala Ala Tyr Glu Phe Ser Gly Lys Asp Asp Phe Gly           
65                  70                  75                  80            

gtt aaa gca gat gtt caa gtg cct ggg gat gat aca cag gta ggt ctg        288
Val Lys Ala Asp Val Gln Val Pro Gly Asp Asp Thr Gln Val Gly Leu           
                85                  90                  95                

att gtc cgt aca aat gat tgg agc caa aaa aat aca tca gac gat ctc        336
Ile Val Arg Thr Asn Asp Trp Ser Gln Lys Asn Thr Ser Asp Asp Leu           
            100                 105                 110                   

cat att gat ctg aca aag ggg cat gaa ata tgg att gtt cag ggg gat        384
His Ile Asp Leu Thr Lys Gly His Glu Ile Trp Ile Val Gln Gly Asp           
        115                 120                 125                       

ccc aat att tat tac aat ctg agt gat gcg cag gct gca gcg act cca        432
Pro Asn Ile Tyr Tyr Asn Leu Ser Asp Ala Gln Ala Ala Ala Thr Pro           
    130                 135                 140                           

aag gtt tcg aat gcg tat ttg gat aat gaa aaa aca gta ttg gca aag        480
Lys Val Ser Asn Ala Tyr Leu Asp Asn Glu Lys Thr Val Leu Ala Lys           
145                 150                 155                 160           

cta act aat cca atg aca tta tca gat gga tca agc ggc ttt acg gtt        528
Leu Thr Asn Pro Met Thr Leu Ser Asp Gly Ser Ser Gly Phe Thr Val           
                165                 170                 175               

aca gat aaa aca aca ggg gaa caa att cca gtt acc gct gca aca aat        576
Thr Asp Lys Thr Thr Gly Glu Gln Ile Pro Val Thr Ala Ala Thr Asn           
            180                 185                 190                   

gcg aac tca gcc tcc tcg tct gag cag aca gac ttg gtt caa ttg acg        624
Ala Asn Ser Ala Ser Ser Ser Glu Gln Thr Asp Leu Val Gln Leu Thr           
        195                 200                 205                       

tta gcc agt gca ccg gat gtt tcc cat aca ata caa gta gga gca gcc        672
Leu Ala Ser Ala Pro Asp Val Ser His Thr Ile Gln Val Gly Ala Ala           
    210                 215                 220                           

ggt tat gaa gca gtc aat ctc ata cca cga aat gta tta aat ttg cct        720
Gly Tyr Glu Ala Val Asn Leu Ile Pro Arg Asn Val Leu Asn Leu Pro           
225                 230                 235                 240           

cgt tat tat tac agc gga aat gat tta ggt aac gtt tat tca aat aag        768
Arg Tyr Tyr Tyr Ser Gly Asn Asp Leu Gly Asn Val Tyr Ser Asn Lys           
                245                 250                 255               

gca acg gcc ttc cgt gta tgg gct cca act gct tcg gat gtc caa tta        816
Ala Thr Ala Phe Arg Val Trp Ala Pro Thr Ala Ser Asp Val Gln Leu           
            260                 265                 270                   

ctt tta tac aat agt gaa aca gga cct gta acc aaa cag ctt gaa atg        864
Leu Leu Tyr Asn Ser Glu Thr Gly Pro Val Thr Lys Gln Leu Glu Met           
        275                 280                 285                       

caa aag agt gat aac ggt aca tgg aaa ctg aag gtc cct ggt aat ctg        912
Gln Lys Ser Asp Asn Gly Thr Trp Lys Leu Lys Val Pro Gly Asn Leu           
    290                 295                 300                           

aaa aat tgg tat tat ctc tat cag gta acg gtg aat ggg aag aca caa        960
Lys Asn Trp Tyr Tyr Leu Tyr Gln Val Thr Val Asn Gly Lys Thr Gln           
305                 310                 315                 320           

aca gcc gtt gac cct tat gtg cgt gct att tca gtc aat gca aca cgt       1008
Thr Ala Val Asp Pro Tyr Val Arg Ala Ile Ser Val Asn Ala Thr Arg           
                325                 330                 335               

ggt atg ata gtc gat tta gaa gat acg aat cct cct gga tgg aaa gaa       1056
Gly Met Ile Val Asp Leu Glu Asp Thr Asn Pro Pro Gly Trp Lys Glu           
            340                 345                 350                   

gat cat caa cag aca cct gcg aac cca gtg gat gaa gta atc tac gaa       1104
Asp His Gln Gln Thr Pro Ala Asn Pro Val Asp Glu Val Ile Tyr Glu           
        355                 360                 365                       

gtg cat gtg cgt gat ttt tcg att gat gct aat tca ggc atg aaa aat       1152
Val His Val Arg Asp Phe Ser Ile Asp Ala Asn Ser Gly Met Lys Asn           
    370                 375                 380                           

aaa ggg aaa tat ctt gcc ttt aca gaa cat ggc aca aaa ggc cct gat       1200
Lys Gly Lys Tyr Leu Ala Phe Thr Glu His Gly Thr Lys Gly Pro Asp           
385                 390                 395                 400           

aac gtg aaa acg ggt att gat agt ttg aag gaa tta gga atc aat gct       1248
Asn Val Lys Thr Gly Ile Asp Ser Leu Lys Glu Leu Gly Ile Asn Ala           
                405                 410                 415               

gtt caa tta cag ccg att gaa gaa ttt aac agc att gat gaa acc caa       1296
Val Gln Leu Gln Pro Ile Glu Glu Phe Asn Ser Ile Asp Glu Thr Gln           
            420                 425                 430                   

cca aat atg tat aac tgg ggc tat gac cca aga aac tac aac gtc cct       1344
Pro Asn Met Tyr Asn Trp Gly Tyr Asp Pro Arg Asn Tyr Asn Val Pro           
        435                 440                 445                       

gaa gga gcg tat gca act aca cca gaa gga acg gct cgc att acc cag       1392
Glu Gly Ala Tyr Ala Thr Thr Pro Glu Gly Thr Ala Arg Ile Thr Gln           
    450                 455                 460                           

tta aag caa ctg att caa agc att cat aaa gat cgg att gct atc aat       1440
Leu Lys Gln Leu Ile Gln Ser Ile His Lys Asp Arg Ile Ala Ile Asn           
465                 470                 475                 480           

atg gat gtg gtc tat aac cat acc ttt aac gta gga gtg tct gat ttt       1488
Met Asp Val Val Tyr Asn His Thr Phe Asn Val Gly Val Ser Asp Phe           
                485                 490                 495               

gat aag att gtt ccg caa tac tat tat cgg aca gac agc gca ggt aat       1536
Asp Lys Ile Val Pro Gln Tyr Tyr Tyr Arg Thr Asp Ser Ala Gly Asn           
            500                 505                 510                   

tat acg aac ggc tca ggt gta ggt aat gaa att gcg acc gag cgt ccg       1584
Tyr Thr Asn Gly Ser Gly Val Gly Asn Glu Ile Ala Thr Glu Arg Pro           
        515                 520                 525                       

atg gtc caa aag ttc gtt ctg gat tct gtt aaa tat tgg gta aag gaa       1632
Met Val Gln Lys Phe Val Leu Asp Ser Val Lys Tyr Trp Val Lys Glu           
    530                 535                 540                           

tac cat atc gac ggc ttc cgt ttc gat ctt atg gct ctt tta gga aaa       1680
Tyr His Ile Asp Gly Phe Arg Phe Asp Leu Met Ala Leu Leu Gly Lys           
545                 550                 555                 560           

gac acc atg gcc aaa ata tca aaa gag ctt cat gct att aat cct ggc       1728
Asp Thr Met Ala Lys Ile Ser Lys Glu Leu His Ala Ile Asn Pro Gly           
                565                 570                 575               

att gtc ctg tat gga gaa cca tgg act ggc ggt acc tct gga tta tca       1776
Ile Val Leu Tyr Gly Glu Pro Trp Thr Gly Gly Thr Ser Gly Leu Ser           
            580                 585                 590                   

agc gac caa ctc gtt acg aaa ggt cag caa aag ggc ttg gga att ggc       1824
Ser Asp Gln Leu Val Thr Lys Gly Gln Gln Lys Gly Leu Gly Ile Gly           
        595                 600                 605                       

gta ttc aac gat aat att cgg aac gga ctc gat ggt aac gtt ttt gat       1872
Val Phe Asn Asp Asn Ile Arg Asn Gly Leu Asp Gly Asn Val Phe Asp           
    610                 615                 620                           

aaa tcg gca caa gga ttt gca aca gga gat cca aac caa gtt aat gtc       1920
Lys Ser Ala Gln Gly Phe Ala Thr Gly Asp Pro Asn Gln Val Asn Val           
625                 630                 635                 640           

att aaa aat gga gtt atg gga agt att tca gat ttc act tcg gca cct       1968
Ile Lys Asn Gly Val Met Gly Ser Ile Ser Asp Phe Thr Ser Ala Pro           
                645                 650                 655               

agc gaa acc att aac tat gta aca agc cat gat aat atg aca ttg tgg       2016
Ser Glu Thr Ile Asn Tyr Val Thr Ser His Asp Asn Met Thr Leu Trp           
            660                 665                 670                   

gat aaa att agc gca agt aat ccg aac gat aca caa gca gat cga att       2064
Asp Lys Ile Ser Ala Ser Asn Pro Asn Asp Thr Gln Ala Asp Arg Ile           
        675                 680                 685                       

aag atg gat gaa ttg gct caa gct gtg gta ttt act tca caa ggg gta       2112
Lys Met Asp Glu Leu Ala Gln Ala Val Val Phe Thr Ser Gln Gly Val           
    690                 695                 700                           

cca ttt atg caa ggt gga gaa gaa atg ctg cgg aca aaa ggc ggt aat       2160
Pro Phe Met Gln Gly Gly Glu Glu Met Leu Arg Thr Lys Gly Gly Asn           
705                 710                 715                 720           

gat aat agt tac aat gcc ggg gat agc gtg aat cag ttc gat tgg tca       2208
Asp Asn Ser Tyr Asn Ala Gly Asp Ser Val Asn Gln Phe Asp Trp Ser           
                725                 730                 735               

aga aaa gca caa ttt gaa aat gta ttc gac tac tat tct tgg ttg att       2256
Arg Lys Ala Gln Phe Glu Asn Val Phe Asp Tyr Tyr Ser Trp Leu Ile           
            740                 745                 750                   

cat cta cgt gat aat cac cca gca ttc cgt atg acg aca gcg gat caa       2304
His Leu Arg Asp Asn His Pro Ala Phe Arg Met Thr Thr Ala Asp Gln           
        755                 760                 765                       

atc aaa caa aat ctc act ttc ttg gat agc cca acg aac act gta gca       2352
Ile Lys Gln Asn Leu Thr Phe Leu Asp Ser Pro Thr Asn Thr Val Ala           
    770                 775                 780                           

ttt gaa tta aaa aat cat gcc aat cat gat aaa tgg aaa aac att ata       2400
Phe Glu Leu Lys Asn His Ala Asn His Asp Lys Trp Lys Asn Ile Ile           
785                 790                 795                 800           

gtt atg tat aat cca aat aaa act gca caa act ctc act cta cca agt       2448
Val Met Tyr Asn Pro Asn Lys Thr Ala Gln Thr Leu Thr Leu Pro Ser           
                805                 810                 815               

gga aat tgg aca att gta gga tta ggc aat caa gta ggt gag aaa tca       2496
Gly Asn Trp Thr Ile Val Gly Leu Gly Asn Gln Val Gly Glu Lys Ser           
            820                 825                 830                   

cta ggc cat gta aat ggc acg gtt gag gtg cca gct ctt agt acg atc       2544
Leu Gly His Val Asn Gly Thr Val Glu Val Pro Ala Leu Ser Thr Ile           
        835                 840                 845                       

att ctt cat cag ggt aca tct gaa gat gtc att gat caa aat               2586
Ile Leu His Gln Gly Thr Ser Glu Asp Val Ile Asp Gln                       
    850                 855                 860                           


<210>  2
<211>  861
<212>  PRT
<213>  Bacillus acidopullulyticus

<400>  2

Met Ser Leu Ile Arg Ser Arg Tyr Asn His Phe Val Ile Leu Phe Thr 
1               5                   10                  15      


Val Ala Ile Met Phe Leu Thr Val Cys Phe Pro Ala Tyr Lys Ala Leu 
            20                  25                  30          


Ala Asp Ser Thr Ser Thr Glu Val Ile Val His Tyr His Arg Phe Asp 
        35                  40                  45              


Ser Asn Tyr Ala Asn Trp Asp Leu Trp Met Trp Pro Tyr Gln Pro Val 
    50                  55                  60                  


Asn Gly Asn Gly Ala Ala Tyr Glu Phe Ser Gly Lys Asp Asp Phe Gly 
65                  70                  75                  80  


Val Lys Ala Asp Val Gln Val Pro Gly Asp Asp Thr Gln Val Gly Leu 
                85                  90                  95      


Ile Val Arg Thr Asn Asp Trp Ser Gln Lys Asn Thr Ser Asp Asp Leu 
            100                 105                 110         


His Ile Asp Leu Thr Lys Gly His Glu Ile Trp Ile Val Gln Gly Asp 
        115                 120                 125             


Pro Asn Ile Tyr Tyr Asn Leu Ser Asp Ala Gln Ala Ala Ala Thr Pro 
    130                 135                 140                 


Lys Val Ser Asn Ala Tyr Leu Asp Asn Glu Lys Thr Val Leu Ala Lys 
145                 150                 155                 160 


Leu Thr Asn Pro Met Thr Leu Ser Asp Gly Ser Ser Gly Phe Thr Val 
                165                 170                 175     


Thr Asp Lys Thr Thr Gly Glu Gln Ile Pro Val Thr Ala Ala Thr Asn 
            180                 185                 190         


Ala Asn Ser Ala Ser Ser Ser Glu Gln Thr Asp Leu Val Gln Leu Thr 
        195                 200                 205             


Leu Ala Ser Ala Pro Asp Val Ser His Thr Ile Gln Val Gly Ala Ala 
    210                 215                 220                 


Gly Tyr Glu Ala Val Asn Leu Ile Pro Arg Asn Val Leu Asn Leu Pro 
225                 230                 235                 240 


Arg Tyr Tyr Tyr Ser Gly Asn Asp Leu Gly Asn Val Tyr Ser Asn Lys 
                245                 250                 255     


Ala Thr Ala Phe Arg Val Trp Ala Pro Thr Ala Ser Asp Val Gln Leu 
            260                 265                 270         


Leu Leu Tyr Asn Ser Glu Thr Gly Pro Val Thr Lys Gln Leu Glu Met 
        275                 280                 285             


Gln Lys Ser Asp Asn Gly Thr Trp Lys Leu Lys Val Pro Gly Asn Leu 
    290                 295                 300                 


Lys Asn Trp Tyr Tyr Leu Tyr Gln Val Thr Val Asn Gly Lys Thr Gln 
305                 310                 315                 320 


Thr Ala Val Asp Pro Tyr Val Arg Ala Ile Ser Val Asn Ala Thr Arg 
                325                 330                 335     


Gly Met Ile Val Asp Leu Glu Asp Thr Asn Pro Pro Gly Trp Lys Glu 
            340                 345                 350         


Asp His Gln Gln Thr Pro Ala Asn Pro Val Asp Glu Val Ile Tyr Glu 
        355                 360                 365             


Val His Val Arg Asp Phe Ser Ile Asp Ala Asn Ser Gly Met Lys Asn 
    370                 375                 380                 


Lys Gly Lys Tyr Leu Ala Phe Thr Glu His Gly Thr Lys Gly Pro Asp 
385                 390                 395                 400 


Asn Val Lys Thr Gly Ile Asp Ser Leu Lys Glu Leu Gly Ile Asn Ala 
                405                 410                 415     


Val Gln Leu Gln Pro Ile Glu Glu Phe Asn Ser Ile Asp Glu Thr Gln 
            420                 425                 430         


Pro Asn Met Tyr Asn Trp Gly Tyr Asp Pro Arg Asn Tyr Asn Val Pro 
        435                 440                 445             


Glu Gly Ala Tyr Ala Thr Thr Pro Glu Gly Thr Ala Arg Ile Thr Gln 
    450                 455                 460                 


Leu Lys Gln Leu Ile Gln Ser Ile His Lys Asp Arg Ile Ala Ile Asn 
465                 470                 475                 480 


Met Asp Val Val Tyr Asn His Thr Phe Asn Val Gly Val Ser Asp Phe 
                485                 490                 495     


Asp Lys Ile Val Pro Gln Tyr Tyr Tyr Arg Thr Asp Ser Ala Gly Asn 
            500                 505                 510         


Tyr Thr Asn Gly Ser Gly Val Gly Asn Glu Ile Ala Thr Glu Arg Pro 
        515                 520                 525             


Met Val Gln Lys Phe Val Leu Asp Ser Val Lys Tyr Trp Val Lys Glu 
    530                 535                 540                 


Tyr His Ile Asp Gly Phe Arg Phe Asp Leu Met Ala Leu Leu Gly Lys 
545                 550                 555                 560 


Asp Thr Met Ala Lys Ile Ser Lys Glu Leu His Ala Ile Asn Pro Gly 
                565                 570                 575     


Ile Val Leu Tyr Gly Glu Pro Trp Thr Gly Gly Thr Ser Gly Leu Ser 
            580                 585                 590         


Ser Asp Gln Leu Val Thr Lys Gly Gln Gln Lys Gly Leu Gly Ile Gly 
        595                 600                 605             


Val Phe Asn Asp Asn Ile Arg Asn Gly Leu Asp Gly Asn Val Phe Asp 
    610                 615                 620                 


Lys Ser Ala Gln Gly Phe Ala Thr Gly Asp Pro Asn Gln Val Asn Val 
625                 630                 635                 640 


Ile Lys Asn Gly Val Met Gly Ser Ile Ser Asp Phe Thr Ser Ala Pro 
                645                 650                 655     


Ser Glu Thr Ile Asn Tyr Val Thr Ser His Asp Asn Met Thr Leu Trp 
            660                 665                 670         


Asp Lys Ile Ser Ala Ser Asn Pro Asn Asp Thr Gln Ala Asp Arg Ile 
        675                 680                 685             


Lys Met Asp Glu Leu Ala Gln Ala Val Val Phe Thr Ser Gln Gly Val 
    690                 695                 700                 


Pro Phe Met Gln Gly Gly Glu Glu Met Leu Arg Thr Lys Gly Gly Asn 
705                 710                 715                 720 


Asp Asn Ser Tyr Asn Ala Gly Asp Ser Val Asn Gln Phe Asp Trp Ser 
                725                 730                 735     


Arg Lys Ala Gln Phe Glu Asn Val Phe Asp Tyr Tyr Ser Trp Leu Ile 
            740                 745                 750         


His Leu Arg Asp Asn His Pro Ala Phe Arg Met Thr Thr Ala Asp Gln 
        755                 760                 765             


Ile Lys Gln Asn Leu Thr Phe Leu Asp Ser Pro Thr Asn Thr Val Ala 
    770                 775                 780                 


Phe Glu Leu Lys Asn His Ala Asn His Asp Lys Trp Lys Asn Ile Ile 
785                 790                 795                 800 


Val Met Tyr Asn Pro Asn Lys Thr Ala Gln Thr Leu Thr Leu Pro Ser 
                805                 810                 815     


Gly Asn Trp Thr Ile Val Gly Leu Gly Asn Gln Val Gly Glu Lys Ser 
            820                 825                 830         


Leu Gly His Val Asn Gly Thr Val Glu Val Pro Ala Leu Ser Thr Ile 
        835                 840                 845             


Ile Leu His Gln Gly Thr Ser Glu Asp Val Ile Asp Gln 
    850                 855                 860     


<210>  3
<211>  2466
<212>  DNA
<213>  Bacillus deramificans


<220>
<221>  CDS
<222>  (1)..(2463)

<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  Since this is a 5' truncated coding sequence, the first codon 
       does NOT encode a methionine but an alanine.

<400>  3
gct gta agc aac gct tat tta gat gct tca aac caa gtt tta gtt aag         48
Ala Val Ser Asn Ala Tyr Leu Asp Ala Ser Asn Gln Val Leu Val Lys           
1               5                   10                  15                

ctt agc caa ccg ttt aca ctc ggt gaa gga gca agc ggc ttc acg gtt         96
Leu Ser Gln Pro Phe Thr Leu Gly Glu Gly Ala Ser Gly Phe Thr Val           
            20                  25                  30                    

cat gat gac acc gta aat aag gat atc cca gtg aca tct gtg acg gat        144
His Asp Asp Thr Val Asn Lys Asp Ile Pro Val Thr Ser Val Thr Asp           
        35                  40                  45                        

gca agt ctt ggt caa aat gta acc gct gtt ttg gca ggt acc ttc caa        192
Ala Ser Leu Gly Gln Asn Val Thr Ala Val Leu Ala Gly Thr Phe Gln           
    50                  55                  60                            

cat att ttt gga ggt tcc gat tgg gca cct gat aat cac agt act tta        240
His Ile Phe Gly Gly Ser Asp Trp Ala Pro Asp Asn His Ser Thr Leu           
65                  70                  75                  80            

tta aaa aag gtg aat aac aat ctc tat caa ttc tca gga gat ctt cct        288
Leu Lys Lys Val Asn Asn Asn Leu Tyr Gln Phe Ser Gly Asp Leu Pro           
                85                  90                  95                

gaa gga aac tac caa tat aaa gtg gct tta aat gat agc tgg aat aat        336
Glu Gly Asn Tyr Gln Tyr Lys Val Ala Leu Asn Asp Ser Trp Asn Asn           
            100                 105                 110                   

ccg agt tac cct tca aac aat atc gat tta acc gta cca aca ggc ggt        384
Pro Ser Tyr Pro Ser Asn Asn Ile Asp Leu Thr Val Pro Thr Gly Gly           
        115                 120                 125                       

gcc cat gtc acc ttt tcc tat gtc ccc tca acg cat gcc gtc tac gac        432
Ala His Val Thr Phe Ser Tyr Val Pro Ser Thr His Ala Val Tyr Asp           
    130                 135                 140                           

agt att aac aac cct ggc gcc gat tta cct gta aat ggc agc ggg gtt        480
Ser Ile Asn Asn Pro Gly Ala Asp Leu Pro Val Asn Gly Ser Gly Val           
145                 150                 155                 160           

aaa acg gat ctc gtg acg gtt act cta ggg gaa gat cca gat gtg agc        528
Lys Thr Asp Leu Val Thr Val Thr Leu Gly Glu Asp Pro Asp Val Ser           
                165                 170                 175               

cat act ctg tcc att caa aca gat ggc tat caa gca aag cag gtg ata        576
His Thr Leu Ser Ile Gln Thr Asp Gly Tyr Gln Ala Lys Gln Val Ile           
            180                 185                 190                   

tct cgt aat gtg ctt gat tca tca cag tat tac tat tca gga gat gat        624
Ser Arg Asn Val Leu Asp Ser Ser Gln Tyr Tyr Tyr Ser Gly Asp Asp           
        195                 200                 205                       

ctt gga aat acc tat aca cat aaa gca act acc ttt aag gtc tgg gca        672
Leu Gly Asn Thr Tyr Thr His Lys Ala Thr Thr Phe Lys Val Trp Ala           
    210                 215                 220                           

cct act tct act caa gta aat gtt ctt ctt tat aat agt gca acg ggt        720
Pro Thr Ser Thr Gln Val Asn Val Leu Leu Tyr Asn Ser Ala Thr Gly           
225                 230                 235                 240           

tct gta aca aaa acc gta cct atg acg gca tcg ggc cat ggt gtg tgg        768
Ser Val Thr Lys Thr Val Pro Met Thr Ala Ser Gly His Gly Val Trp           
                245                 250                 255               

gaa gca acg gtt aat caa aac ctt gaa aat tgg tat tac atg tat gag        816
Glu Ala Thr Val Asn Gln Asn Leu Glu Asn Trp Tyr Tyr Met Tyr Glu           
            260                 265                 270                   

gta aca ggc caa ggc tct acc cga acg gct gtt gat cct tat gca act        864
Val Thr Gly Gln Gly Ser Thr Arg Thr Ala Val Asp Pro Tyr Ala Thr           
        275                 280                 285                       

gcg att gca cca aat gga acg aga ggc atg att gtg gac ctg gct aaa        912
Ala Ile Ala Pro Asn Gly Thr Arg Gly Met Ile Val Asp Leu Ala Lys           
    290                 295                 300                           

aca gat cct gct ggc tgg aac agt gat aaa cat att acg cca aag aat        960
Thr Asp Pro Ala Gly Trp Asn Ser Asp Lys His Ile Thr Pro Lys Asn           
305                 310                 315                 320           

ata gaa gat gag gtc atc tat gaa atg gat gtc cgt gac ttt tcc att       1008
Ile Glu Asp Glu Val Ile Tyr Glu Met Asp Val Arg Asp Phe Ser Ile           
                325                 330                 335               

gac cct aat tcg ggt atg aaa aat aaa ggg aag tat ttg gct ctt aca       1056
Asp Pro Asn Ser Gly Met Lys Asn Lys Gly Lys Tyr Leu Ala Leu Thr           
            340                 345                 350                   

gaa aaa gga aca aag ggc cct gac aac gta aag acg ggg ata gat tcc       1104
Glu Lys Gly Thr Lys Gly Pro Asp Asn Val Lys Thr Gly Ile Asp Ser           
        355                 360                 365                       

tta aaa caa ctt ggg att act cat gtt cag ctt atg cct gtt ttc gca       1152
Leu Lys Gln Leu Gly Ile Thr His Val Gln Leu Met Pro Val Phe Ala           
    370                 375                 380                           

ttt aac agt gtc gat gaa act gat cca acc caa gat aat tgg ggt tat       1200
Phe Asn Ser Val Asp Glu Thr Asp Pro Thr Gln Asp Asn Trp Gly Tyr           
385                 390                 395                 400           

gac cct cgc aac tat gat gtt cct gaa ggg cag tat gct aca aat gcg       1248
Asp Pro Arg Asn Tyr Asp Val Pro Glu Gly Gln Tyr Ala Thr Asn Ala           
                405                 410                 415               

aat ggt acg gct cgt ata aaa gag ttt aag gaa atg gtt ctt tca ctc       1296
Asn Gly Thr Ala Arg Ile Lys Glu Phe Lys Glu Met Val Leu Ser Leu           
            420                 425                 430                   

cat cgt gaa cac att ggg gtt aac atg gat gtt gtc tat aat cat acc       1344
His Arg Glu His Ile Gly Val Asn Met Asp Val Val Tyr Asn His Thr           
        435                 440                 445                       

ttt gcc acg caa atc tct gac ttc gat aaa att gta cca gaa tat tat       1392
Phe Ala Thr Gln Ile Ser Asp Phe Asp Lys Ile Val Pro Glu Tyr Tyr           
    450                 455                 460                           

tac cgt acg gat gat gca ggt aat tat acc aac gga tca ggt act gga       1440
Tyr Arg Thr Asp Asp Ala Gly Asn Tyr Thr Asn Gly Ser Gly Thr Gly           
465                 470                 475                 480           

aat gaa atc gca gcc gaa agg cca atg gtt caa aaa ttt att att gat       1488
Asn Glu Ile Ala Ala Glu Arg Pro Met Val Gln Lys Phe Ile Ile Asp           
                485                 490                 495               

tcc ctt aag tat tgg gtc aat gag tat cat att gac ggc ttc cgt ttt       1536
Ser Leu Lys Tyr Trp Val Asn Glu Tyr His Ile Asp Gly Phe Arg Phe           
            500                 505                 510                   

gac tta atg gcg ctg ctt gga aaa gac acg atg tcg aaa gct gcc tcg       1584
Asp Leu Met Ala Leu Leu Gly Lys Asp Thr Met Ser Lys Ala Ala Ser           
        515                 520                 525                       

gag ctt cat gct att aat cca gga att gca ctt tac ggt gag cca tgg       1632
Glu Leu His Ala Ile Asn Pro Gly Ile Ala Leu Tyr Gly Glu Pro Trp           
    530                 535                 540                           

acg ggt gga acc tct gca ctg cca gaa gat cag ctt ctg aca aaa gga       1680
Thr Gly Gly Thr Ser Ala Leu Pro Glu Asp Gln Leu Leu Thr Lys Gly           
545                 550                 555                 560           

gct caa aaa ggc atg gga gta gcg gtg ttt aat gac aat tta cga aac       1728
Ala Gln Lys Gly Met Gly Val Ala Val Phe Asn Asp Asn Leu Arg Asn           
                565                 570                 575               

gcg ttg gac ggc aat gtc ttt gat tct tcc gct caa ggt ttt gcg aca       1776
Ala Leu Asp Gly Asn Val Phe Asp Ser Ser Ala Gln Gly Phe Ala Thr           
            580                 585                 590                   

ggt gca aca ggc tta act gat gca att aag aat ggc gtt gag ggg agt       1824
Gly Ala Thr Gly Leu Thr Asp Ala Ile Lys Asn Gly Val Glu Gly Ser           
        595                 600                 605                       

att aat gac ttt acc tct tca cca ggt gag aca att aac tat gtc aca       1872
Ile Asn Asp Phe Thr Ser Ser Pro Gly Glu Thr Ile Asn Tyr Val Thr           
    610                 615                 620                           

agt cat gat aac tac acc ctt tgg gac aaa ata gcc cta agc aac cct       1920
Ser His Asp Asn Tyr Thr Leu Trp Asp Lys Ile Ala Leu Ser Asn Pro           
625                 630                 635                 640           

aat gat tcc gaa gcg gat cgg att aaa atg gat gaa ctc gca caa gca       1968
Asn Asp Ser Glu Ala Asp Arg Ile Lys Met Asp Glu Leu Ala Gln Ala           
                645                 650                 655               

gtt gtt atg acc tca caa ggt gtt cca ttc atg caa ggc ggg gaa gaa       2016
Val Val Met Thr Ser Gln Gly Val Pro Phe Met Gln Gly Gly Glu Glu           
            660                 665                 670                   

atg ctt cgt aca aaa ggc ggc aac gac aat agt tat aat gca ggc gat       2064
Met Leu Arg Thr Lys Gly Gly Asn Asp Asn Ser Tyr Asn Ala Gly Asp           
        675                 680                 685                       

acg gtc aat gag ttt gat tgg agc agg aaa gct caa tat cca gat gtt       2112
Thr Val Asn Glu Phe Asp Trp Ser Arg Lys Ala Gln Tyr Pro Asp Val           
    690                 695                 700                           

ttc aac tat tat agc ggg cta atc cac ctt cgt ctt gat cac cca gcc       2160
Phe Asn Tyr Tyr Ser Gly Leu Ile His Leu Arg Leu Asp His Pro Ala           
705                 710                 715                 720           

ttc cgc atg acg aca gct aat gaa atc aat agc cac ctc caa ttc cta       2208
Phe Arg Met Thr Thr Ala Asn Glu Ile Asn Ser His Leu Gln Phe Leu           
                725                 730                 735               

aat agt cca gag aac aca gtg gcc tat gaa tta act gat cat gtt aat       2256
Asn Ser Pro Glu Asn Thr Val Ala Tyr Glu Leu Thr Asp His Val Asn           
            740                 745                 750                   

aaa gac aaa tgg gga aat atc att gtt gtt tat aac cca aat aaa act       2304
Lys Asp Lys Trp Gly Asn Ile Ile Val Val Tyr Asn Pro Asn Lys Thr           
        755                 760                 765                       

gca gca acc att aat ttg ccg agc ggg aaa tgg gca atc aat gct acg       2352
Ala Ala Thr Ile Asn Leu Pro Ser Gly Lys Trp Ala Ile Asn Ala Thr           
    770                 775                 780                           

agc ggt aag gta gga gaa tcc acc ctt ggt caa gca gag gga agt gtc       2400
Ser Gly Lys Val Gly Glu Ser Thr Leu Gly Gln Ala Glu Gly Ser Val           
785                 790                 795                 800           

caa gta cca ggt ata tct atg atg atc ctt cat caa gag gta agc cca       2448
Gln Val Pro Gly Ile Ser Met Met Ile Leu His Gln Glu Val Ser Pro           
                805                 810                 815               

gac cac ggt aaa aag taa                                               2466
Asp His Gly Lys Lys                                                       
            820                                                           


<210>  4
<211>  821
<212>  PRT
<213>  Bacillus deramificans

<400>  4

Ala Val Ser Asn Ala Tyr Leu Asp Ala Ser Asn Gln Val Leu Val Lys 
1               5                   10                  15      


Leu Ser Gln Pro Phe Thr Leu Gly Glu Gly Ala Ser Gly Phe Thr Val 
            20                  25                  30          


His Asp Asp Thr Val Asn Lys Asp Ile Pro Val Thr Ser Val Thr Asp 
        35                  40                  45              


Ala Ser Leu Gly Gln Asn Val Thr Ala Val Leu Ala Gly Thr Phe Gln 
    50                  55                  60                  


His Ile Phe Gly Gly Ser Asp Trp Ala Pro Asp Asn His Ser Thr Leu 
65                  70                  75                  80  


Leu Lys Lys Val Asn Asn Asn Leu Tyr Gln Phe Ser Gly Asp Leu Pro 
                85                  90                  95      


Glu Gly Asn Tyr Gln Tyr Lys Val Ala Leu Asn Asp Ser Trp Asn Asn 
            100                 105                 110         


Pro Ser Tyr Pro Ser Asn Asn Ile Asp Leu Thr Val Pro Thr Gly Gly 
        115                 120                 125             


Ala His Val Thr Phe Ser Tyr Val Pro Ser Thr His Ala Val Tyr Asp 
    130                 135                 140                 


Ser Ile Asn Asn Pro Gly Ala Asp Leu Pro Val Asn Gly Ser Gly Val 
145                 150                 155                 160 


Lys Thr Asp Leu Val Thr Val Thr Leu Gly Glu Asp Pro Asp Val Ser 
                165                 170                 175     


His Thr Leu Ser Ile Gln Thr Asp Gly Tyr Gln Ala Lys Gln Val Ile 
            180                 185                 190         


Ser Arg Asn Val Leu Asp Ser Ser Gln Tyr Tyr Tyr Ser Gly Asp Asp 
        195                 200                 205             


Leu Gly Asn Thr Tyr Thr His Lys Ala Thr Thr Phe Lys Val Trp Ala 
    210                 215                 220                 


Pro Thr Ser Thr Gln Val Asn Val Leu Leu Tyr Asn Ser Ala Thr Gly 
225                 230                 235                 240 


Ser Val Thr Lys Thr Val Pro Met Thr Ala Ser Gly His Gly Val Trp 
                245                 250                 255     


Glu Ala Thr Val Asn Gln Asn Leu Glu Asn Trp Tyr Tyr Met Tyr Glu 
            260                 265                 270         


Val Thr Gly Gln Gly Ser Thr Arg Thr Ala Val Asp Pro Tyr Ala Thr 
        275                 280                 285             


Ala Ile Ala Pro Asn Gly Thr Arg Gly Met Ile Val Asp Leu Ala Lys 
    290                 295                 300                 


Thr Asp Pro Ala Gly Trp Asn Ser Asp Lys His Ile Thr Pro Lys Asn 
305                 310                 315                 320 


Ile Glu Asp Glu Val Ile Tyr Glu Met Asp Val Arg Asp Phe Ser Ile 
                325                 330                 335     


Asp Pro Asn Ser Gly Met Lys Asn Lys Gly Lys Tyr Leu Ala Leu Thr 
            340                 345                 350         


Glu Lys Gly Thr Lys Gly Pro Asp Asn Val Lys Thr Gly Ile Asp Ser 
        355                 360                 365             


Leu Lys Gln Leu Gly Ile Thr His Val Gln Leu Met Pro Val Phe Ala 
    370                 375                 380                 


Phe Asn Ser Val Asp Glu Thr Asp Pro Thr Gln Asp Asn Trp Gly Tyr 
385                 390                 395                 400 


Asp Pro Arg Asn Tyr Asp Val Pro Glu Gly Gln Tyr Ala Thr Asn Ala 
                405                 410                 415     


Asn Gly Thr Ala Arg Ile Lys Glu Phe Lys Glu Met Val Leu Ser Leu 
            420                 425                 430         


His Arg Glu His Ile Gly Val Asn Met Asp Val Val Tyr Asn His Thr 
        435                 440                 445             


Phe Ala Thr Gln Ile Ser Asp Phe Asp Lys Ile Val Pro Glu Tyr Tyr 
    450                 455                 460                 


Tyr Arg Thr Asp Asp Ala Gly Asn Tyr Thr Asn Gly Ser Gly Thr Gly 
465                 470                 475                 480 


Asn Glu Ile Ala Ala Glu Arg Pro Met Val Gln Lys Phe Ile Ile Asp 
                485                 490                 495     


Ser Leu Lys Tyr Trp Val Asn Glu Tyr His Ile Asp Gly Phe Arg Phe 
            500                 505                 510         


Asp Leu Met Ala Leu Leu Gly Lys Asp Thr Met Ser Lys Ala Ala Ser 
        515                 520                 525             


Glu Leu His Ala Ile Asn Pro Gly Ile Ala Leu Tyr Gly Glu Pro Trp 
    530                 535                 540                 


Thr Gly Gly Thr Ser Ala Leu Pro Glu Asp Gln Leu Leu Thr Lys Gly 
545                 550                 555                 560 


Ala Gln Lys Gly Met Gly Val Ala Val Phe Asn Asp Asn Leu Arg Asn 
                565                 570                 575     


Ala Leu Asp Gly Asn Val Phe Asp Ser Ser Ala Gln Gly Phe Ala Thr 
            580                 585                 590         


Gly Ala Thr Gly Leu Thr Asp Ala Ile Lys Asn Gly Val Glu Gly Ser 
        595                 600                 605             


Ile Asn Asp Phe Thr Ser Ser Pro Gly Glu Thr Ile Asn Tyr Val Thr 
    610                 615                 620                 


Ser His Asp Asn Tyr Thr Leu Trp Asp Lys Ile Ala Leu Ser Asn Pro 
625                 630                 635                 640 


Asn Asp Ser Glu Ala Asp Arg Ile Lys Met Asp Glu Leu Ala Gln Ala 
                645                 650                 655     


Val Val Met Thr Ser Gln Gly Val Pro Phe Met Gln Gly Gly Glu Glu 
            660                 665                 670         


Met Leu Arg Thr Lys Gly Gly Asn Asp Asn Ser Tyr Asn Ala Gly Asp 
        675                 680                 685             


Thr Val Asn Glu Phe Asp Trp Ser Arg Lys Ala Gln Tyr Pro Asp Val 
    690                 695                 700                 


Phe Asn Tyr Tyr Ser Gly Leu Ile His Leu Arg Leu Asp His Pro Ala 
705                 710                 715                 720 


Phe Arg Met Thr Thr Ala Asn Glu Ile Asn Ser His Leu Gln Phe Leu 
                725                 730                 735     


Asn Ser Pro Glu Asn Thr Val Ala Tyr Glu Leu Thr Asp His Val Asn 
            740                 745                 750         


Lys Asp Lys Trp Gly Asn Ile Ile Val Val Tyr Asn Pro Asn Lys Thr 
        755                 760                 765             


Ala Ala Thr Ile Asn Leu Pro Ser Gly Lys Trp Ala Ile Asn Ala Thr 
    770                 775                 780                 


Ser Gly Lys Val Gly Glu Ser Thr Leu Gly Gln Ala Glu Gly Ser Val 
785                 790                 795                 800 


Gln Val Pro Gly Ile Ser Met Met Ile Leu His Gln Glu Val Ser Pro 
                805                 810                 815     


Asp His Gly Lys Lys 
            820     


<210>  5
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  FORWARD PRIMER

<400>  5
cggaacgcct ggctgacaac acg                                               23


<210>  6
<211>  44
<212>  DNA
<213>  artificial sequence

<220>
<223>  Variant1R

<400>  6
atccaaatac gcattcgaaa cagcagccga tgcgatcgat gaac                        44


<210>  7
<211>  44
<212>  DNA
<213>  artificial sequence

<220>
<223>  Variant2R

<400>  7
ctgtaataat aacgaggcaa attaagcaca ttacgagata tcac                        44


<210>  8
<211>  45
<212>  DNA
<213>  artificial sequence

<220>
<223>  Variant3R

<400>  8
catccaggag gattcgtatc ttccaggtcc acaatcatgc ctctc                       45


<210>  9
<211>  45
<212>  DNA
<213>  artificial sequence

<220>
<223>  Variant4R

<400>  9
gttaccatcg agtccgttcc gaatattgtc attaaacacc gctac                       45


<210>  10
<211>  44
<212>  DNA
<213>  artificial sequence

<220>
<223>  Variant5R

<400>  10
tctaaataag cgttgcttac agcctttgga gtcgctgcag cctg                        44


<210>  11
<211>  45
<212>  DNA
<213>  artificial sequence

<220>
<223>  Variant6R

<400>  11
ctgtgatgaa tcaagcacat tacgtggtat gagattgact gcttc                       45


<210>  12
<211>  45
<212>  DNA
<213>  artificial sequence

<220>
<223>  Variant7R

<400>  12
caggtccaca atcatgcctc tcgttgcatt gactgaaata gcacg                       45


<210>  13
<211>  45
<212>  DNA
<213>  artificial sequence

<220>
<223>  Variant8R

<400>  13
gtttcgtaaa ttgtcattaa acacgccaat tcccaagccc ttttg                       45


<210>  14
<211>  27
<212>  DNA
<213>  artificial sequence

<220>
<223>  REVERSE PRIMER

<400>  14
caatccaaga gaaccctgat acggatg                                           27


<210>  15
<211>  2586
<212>  DNA
<213>  artificial sequence

<220>
<223>  Chimeric variant8


<220>
<221>  CDS
<222>  (1)..(2583)
<223>  Encodes the Variant8 pullulanase.

<400>  15
atg tcc cta ata cgt tct agg tat aat cat ttt gtc att ctt ttt act         48
Met Ser Leu Ile Arg Ser Arg Tyr Asn His Phe Val Ile Leu Phe Thr           
1               5                   10                  15                

gtc gcc ata atg ttt cta aca gtt tgt ttc ccc gct tat aaa gct tta         96
Val Ala Ile Met Phe Leu Thr Val Cys Phe Pro Ala Tyr Lys Ala Leu           
            20                  25                  30                    

gca gat tct acc tcg aca gaa gtc att gtg cat tat cat cgt ttt gat        144
Ala Asp Ser Thr Ser Thr Glu Val Ile Val His Tyr His Arg Phe Asp           
        35                  40                  45                        

tct aac tat gca aat tgg gat cta tgg atg tgg cca tat caa cca gtt        192
Ser Asn Tyr Ala Asn Trp Asp Leu Trp Met Trp Pro Tyr Gln Pro Val           
    50                  55                  60                            

aat ggt aat gga gca gca tac gag ttt tct gga aag gat gat ttt ggc        240
Asn Gly Asn Gly Ala Ala Tyr Glu Phe Ser Gly Lys Asp Asp Phe Gly           
65                  70                  75                  80            

gtt aaa gca gat gtt caa gtg cct ggg gat gat aca cag gta ggt ctg        288
Val Lys Ala Asp Val Gln Val Pro Gly Asp Asp Thr Gln Val Gly Leu           
                85                  90                  95                

att gtc cgt aca aat gat tgg agc caa aaa aat aca tca gac gat ctc        336
Ile Val Arg Thr Asn Asp Trp Ser Gln Lys Asn Thr Ser Asp Asp Leu           
            100                 105                 110                   

cat att gat ctg aca aag ggg cat gaa ata tgg att gtt cag ggg gat        384
His Ile Asp Leu Thr Lys Gly His Glu Ile Trp Ile Val Gln Gly Asp           
        115                 120                 125                       

ccc aat att tat tac aat ctg agt gat gcg cag gct gca gcg act cca        432
Pro Asn Ile Tyr Tyr Asn Leu Ser Asp Ala Gln Ala Ala Ala Thr Pro           
    130                 135                 140                           

aag gtt tcg aat gcg tat ttg gat aat gaa aaa aca gta ttg gca aag        480
Lys Val Ser Asn Ala Tyr Leu Asp Asn Glu Lys Thr Val Leu Ala Lys           
145                 150                 155                 160           

cta act aat cca atg aca tta tca gat gga tca agc ggc ttt acg gtt        528
Leu Thr Asn Pro Met Thr Leu Ser Asp Gly Ser Ser Gly Phe Thr Val           
                165                 170                 175               

aca gat aaa aca aca ggg gaa caa att cca gtt acc gct gca aca aat        576
Thr Asp Lys Thr Thr Gly Glu Gln Ile Pro Val Thr Ala Ala Thr Asn           
            180                 185                 190                   

gcg aac tca gcc tcc tcg tct gag cag aca gac ttg gtt caa ttg acg        624
Ala Asn Ser Ala Ser Ser Ser Glu Gln Thr Asp Leu Val Gln Leu Thr           
        195                 200                 205                       

tta gcc agt gca ccg gat gtt tcc cat aca ata caa gta gga gca gcc        672
Leu Ala Ser Ala Pro Asp Val Ser His Thr Ile Gln Val Gly Ala Ala           
    210                 215                 220                           

ggt tat gaa gca gtc aat ctc ata cca cga aat gta tta aat ttg cct        720
Gly Tyr Glu Ala Val Asn Leu Ile Pro Arg Asn Val Leu Asn Leu Pro           
225                 230                 235                 240           

cgt tat tat tac agc gga aat gat tta ggt aac gtt tat tca aat aag        768
Arg Tyr Tyr Tyr Ser Gly Asn Asp Leu Gly Asn Val Tyr Ser Asn Lys           
                245                 250                 255               

gca acg gcc ttc cgt gta tgg gct cca act gct tcg gat gtc caa tta        816
Ala Thr Ala Phe Arg Val Trp Ala Pro Thr Ala Ser Asp Val Gln Leu           
            260                 265                 270                   

ctt tta tac aat agt gaa aca gga cct gta acc aaa cag ctt gaa atg        864
Leu Leu Tyr Asn Ser Glu Thr Gly Pro Val Thr Lys Gln Leu Glu Met           
        275                 280                 285                       

caa aag agt gat aac ggt aca tgg aaa ctg aag gtc cct ggt aat ctg        912
Gln Lys Ser Asp Asn Gly Thr Trp Lys Leu Lys Val Pro Gly Asn Leu           
    290                 295                 300                           

aaa aat tgg tat tat ctc tat cag gta acg gtg aat ggg aag aca caa        960
Lys Asn Trp Tyr Tyr Leu Tyr Gln Val Thr Val Asn Gly Lys Thr Gln           
305                 310                 315                 320           

aca gcc gtt gac cct tat gtg cgt gct att tca gtc aat gca aca cgt       1008
Thr Ala Val Asp Pro Tyr Val Arg Ala Ile Ser Val Asn Ala Thr Arg           
                325                 330                 335               

ggt atg ata gtc gat tta gaa gat acg aat cct cct gga tgg aaa gaa       1056
Gly Met Ile Val Asp Leu Glu Asp Thr Asn Pro Pro Gly Trp Lys Glu           
            340                 345                 350                   

gat cat caa cag aca cct gcg aac cca gtg gat gaa gta atc tac gaa       1104
Asp His Gln Gln Thr Pro Ala Asn Pro Val Asp Glu Val Ile Tyr Glu           
        355                 360                 365                       

gtg cat gtg cgt gat ttt tcg att gat gct aat tca ggc atg aaa aat       1152
Val His Val Arg Asp Phe Ser Ile Asp Ala Asn Ser Gly Met Lys Asn           
    370                 375                 380                           

aaa ggg aaa tat ctt gcc ttt aca gaa cat ggc aca aaa ggc cct gat       1200
Lys Gly Lys Tyr Leu Ala Phe Thr Glu His Gly Thr Lys Gly Pro Asp           
385                 390                 395                 400           

aac gtg aaa acg ggt att gat agt ttg aag gaa tta gga atc aat gct       1248
Asn Val Lys Thr Gly Ile Asp Ser Leu Lys Glu Leu Gly Ile Asn Ala           
                405                 410                 415               

gtt caa tta cag ccg att gaa gaa ttt aac agc att gat gaa acc caa       1296
Val Gln Leu Gln Pro Ile Glu Glu Phe Asn Ser Ile Asp Glu Thr Gln           
            420                 425                 430                   

cca aat atg tat aac tgg ggc tat gac cca aga aac tac aac gtc cct       1344
Pro Asn Met Tyr Asn Trp Gly Tyr Asp Pro Arg Asn Tyr Asn Val Pro           
        435                 440                 445                       

gaa gga gcg tat gca act aca cca gaa gga acg gct cgc att acc cag       1392
Glu Gly Ala Tyr Ala Thr Thr Pro Glu Gly Thr Ala Arg Ile Thr Gln           
    450                 455                 460                           

tta aag caa ctg att caa agc att cat aaa gat cgg att gct atc aat       1440
Leu Lys Gln Leu Ile Gln Ser Ile His Lys Asp Arg Ile Ala Ile Asn           
465                 470                 475                 480           

atg gat gtg gtc tat aac cat acc ttt aac gta gga gtg tct gat ttt       1488
Met Asp Val Val Tyr Asn His Thr Phe Asn Val Gly Val Ser Asp Phe           
                485                 490                 495               

gat aag att gtt ccg caa tac tat tat cgg aca gac agc gca ggt aat       1536
Asp Lys Ile Val Pro Gln Tyr Tyr Tyr Arg Thr Asp Ser Ala Gly Asn           
            500                 505                 510                   

tat acg aac ggc tca ggt gta ggt aat gaa att gcg acc gag cgt ccg       1584
Tyr Thr Asn Gly Ser Gly Val Gly Asn Glu Ile Ala Thr Glu Arg Pro           
        515                 520                 525                       

atg gtc caa aag ttc gtt ctg gat tct gtt aaa tat tgg gta aag gaa       1632
Met Val Gln Lys Phe Val Leu Asp Ser Val Lys Tyr Trp Val Lys Glu           
    530                 535                 540                           

tac cat atc gac ggc ttc cgt ttc gat ctt atg gct ctt tta gga aaa       1680
Tyr His Ile Asp Gly Phe Arg Phe Asp Leu Met Ala Leu Leu Gly Lys           
545                 550                 555                 560           

gac acc atg gcc aaa ata tca aaa gag ctt cat gct att aat cct ggc       1728
Asp Thr Met Ala Lys Ile Ser Lys Glu Leu His Ala Ile Asn Pro Gly           
                565                 570                 575               

att gtc ctg tat gga gaa cca tgg act ggc ggt acc tct gga tta tca       1776
Ile Val Leu Tyr Gly Glu Pro Trp Thr Gly Gly Thr Ser Gly Leu Ser           
            580                 585                 590                   

agc gac caa ctc gtt acg aaa ggt cag caa aag ggc ttg gga att ggc       1824
Ser Asp Gln Leu Val Thr Lys Gly Gln Gln Lys Gly Leu Gly Ile Gly           
        595                 600                 605                       

gtg ttt aat gac aat tta cga aac gcg ttg gac ggc aat gtc ttt gat       1872
Val Phe Asn Asp Asn Leu Arg Asn Ala Leu Asp Gly Asn Val Phe Asp           
    610                 615                 620                           

tct tcc gct caa ggt ttt gcg aca ggt gca aca ggc tta act gat gca       1920
Ser Ser Ala Gln Gly Phe Ala Thr Gly Ala Thr Gly Leu Thr Asp Ala           
625                 630                 635                 640           

att aag aat ggc gtt gag ggg agt att aat gac ttt acc tct tca cca       1968
Ile Lys Asn Gly Val Glu Gly Ser Ile Asn Asp Phe Thr Ser Ser Pro           
                645                 650                 655               

ggt gag aca att aac tat gtc aca agt cat gat aac tac acc ctt tgg       2016
Gly Glu Thr Ile Asn Tyr Val Thr Ser His Asp Asn Tyr Thr Leu Trp           
            660                 665                 670                   

gac aaa ata gcc cta agc aac cct aat gat tcc gaa gcg gat cgg att       2064
Asp Lys Ile Ala Leu Ser Asn Pro Asn Asp Ser Glu Ala Asp Arg Ile           
        675                 680                 685                       

aaa atg gat gaa ctc gca caa gca gtt gtt atg acc tca caa ggt gtt       2112
Lys Met Asp Glu Leu Ala Gln Ala Val Val Met Thr Ser Gln Gly Val           
    690                 695                 700                           

cca ttc atg caa ggc ggg gaa gaa atg ctt cgt aca aaa ggc ggc aac       2160
Pro Phe Met Gln Gly Gly Glu Glu Met Leu Arg Thr Lys Gly Gly Asn           
705                 710                 715                 720           

gac aat agt tat aat gca ggc gat acg gtc aat gag ttt gat tgg agc       2208
Asp Asn Ser Tyr Asn Ala Gly Asp Thr Val Asn Glu Phe Asp Trp Ser           
                725                 730                 735               

agg aaa gct caa tat cca gat gtt ttc aac tat tat agc ggg cta atc       2256
Arg Lys Ala Gln Tyr Pro Asp Val Phe Asn Tyr Tyr Ser Gly Leu Ile           
            740                 745                 750                   

cac ctt cgt ctt gat cac cca gcc ttc cgc atg acg aca gct aat gaa       2304
His Leu Arg Leu Asp His Pro Ala Phe Arg Met Thr Thr Ala Asn Glu           
        755                 760                 765                       

atc aat agc cac ctc caa ttc cta aat agt cca gag aac aca gtg gcc       2352
Ile Asn Ser His Leu Gln Phe Leu Asn Ser Pro Glu Asn Thr Val Ala           
    770                 775                 780                           

tat gaa tta act gat cat gtt aat aaa gac aaa tgg gga aat atc att       2400
Tyr Glu Leu Thr Asp His Val Asn Lys Asp Lys Trp Gly Asn Ile Ile           
785                 790                 795                 800           

gtt gtt tat aac cca aat aaa act gca gca acc att aat ttg ccg agc       2448
Val Val Tyr Asn Pro Asn Lys Thr Ala Ala Thr Ile Asn Leu Pro Ser           
                805                 810                 815               

ggg aaa tgg gca atc aat gct acg agc ggt aag gta gga gaa tcc acc       2496
Gly Lys Trp Ala Ile Asn Ala Thr Ser Gly Lys Val Gly Glu Ser Thr           
            820                 825                 830                   

ctt ggt caa gca gag gga agt gtt caa gtc cca ggt ata tct atg atg       2544
Leu Gly Gln Ala Glu Gly Ser Val Gln Val Pro Gly Ile Ser Met Met           
        835                 840                 845                       

atc ctt cat caa gag gta agc cca gac cac ggt aaa aag taa               2586
Ile Leu His Gln Glu Val Ser Pro Asp His Gly Lys Lys                       
    850                 855                 860                           


<210>  16
<211>  861
<212>  PRT
<213>  artificial sequence

<220>
<223>  Synthetic Construct

<400>  16

Met Ser Leu Ile Arg Ser Arg Tyr Asn His Phe Val Ile Leu Phe Thr 
1               5                   10                  15      


Val Ala Ile Met Phe Leu Thr Val Cys Phe Pro Ala Tyr Lys Ala Leu 
            20                  25                  30          


Ala Asp Ser Thr Ser Thr Glu Val Ile Val His Tyr His Arg Phe Asp 
        35                  40                  45              


Ser Asn Tyr Ala Asn Trp Asp Leu Trp Met Trp Pro Tyr Gln Pro Val 
    50                  55                  60                  


Asn Gly Asn Gly Ala Ala Tyr Glu Phe Ser Gly Lys Asp Asp Phe Gly 
65                  70                  75                  80  


Val Lys Ala Asp Val Gln Val Pro Gly Asp Asp Thr Gln Val Gly Leu 
                85                  90                  95      


Ile Val Arg Thr Asn Asp Trp Ser Gln Lys Asn Thr Ser Asp Asp Leu 
            100                 105                 110         


His Ile Asp Leu Thr Lys Gly His Glu Ile Trp Ile Val Gln Gly Asp 
        115                 120                 125             


Pro Asn Ile Tyr Tyr Asn Leu Ser Asp Ala Gln Ala Ala Ala Thr Pro 
    130                 135                 140                 


Lys Val Ser Asn Ala Tyr Leu Asp Asn Glu Lys Thr Val Leu Ala Lys 
145                 150                 155                 160 


Leu Thr Asn Pro Met Thr Leu Ser Asp Gly Ser Ser Gly Phe Thr Val 
                165                 170                 175     


Thr Asp Lys Thr Thr Gly Glu Gln Ile Pro Val Thr Ala Ala Thr Asn 
            180                 185                 190         


Ala Asn Ser Ala Ser Ser Ser Glu Gln Thr Asp Leu Val Gln Leu Thr 
        195                 200                 205             


Leu Ala Ser Ala Pro Asp Val Ser His Thr Ile Gln Val Gly Ala Ala 
    210                 215                 220                 


Gly Tyr Glu Ala Val Asn Leu Ile Pro Arg Asn Val Leu Asn Leu Pro 
225                 230                 235                 240 


Arg Tyr Tyr Tyr Ser Gly Asn Asp Leu Gly Asn Val Tyr Ser Asn Lys 
                245                 250                 255     


Ala Thr Ala Phe Arg Val Trp Ala Pro Thr Ala Ser Asp Val Gln Leu 
            260                 265                 270         


Leu Leu Tyr Asn Ser Glu Thr Gly Pro Val Thr Lys Gln Leu Glu Met 
        275                 280                 285             


Gln Lys Ser Asp Asn Gly Thr Trp Lys Leu Lys Val Pro Gly Asn Leu 
    290                 295                 300                 


Lys Asn Trp Tyr Tyr Leu Tyr Gln Val Thr Val Asn Gly Lys Thr Gln 
305                 310                 315                 320 


Thr Ala Val Asp Pro Tyr Val Arg Ala Ile Ser Val Asn Ala Thr Arg 
                325                 330                 335     


Gly Met Ile Val Asp Leu Glu Asp Thr Asn Pro Pro Gly Trp Lys Glu 
            340                 345                 350         


Asp His Gln Gln Thr Pro Ala Asn Pro Val Asp Glu Val Ile Tyr Glu 
        355                 360                 365             


Val His Val Arg Asp Phe Ser Ile Asp Ala Asn Ser Gly Met Lys Asn 
    370                 375                 380                 


Lys Gly Lys Tyr Leu Ala Phe Thr Glu His Gly Thr Lys Gly Pro Asp 
385                 390                 395                 400 


Asn Val Lys Thr Gly Ile Asp Ser Leu Lys Glu Leu Gly Ile Asn Ala 
                405                 410                 415     


Val Gln Leu Gln Pro Ile Glu Glu Phe Asn Ser Ile Asp Glu Thr Gln 
            420                 425                 430         


Pro Asn Met Tyr Asn Trp Gly Tyr Asp Pro Arg Asn Tyr Asn Val Pro 
        435                 440                 445             


Glu Gly Ala Tyr Ala Thr Thr Pro Glu Gly Thr Ala Arg Ile Thr Gln 
    450                 455                 460                 


Leu Lys Gln Leu Ile Gln Ser Ile His Lys Asp Arg Ile Ala Ile Asn 
465                 470                 475                 480 


Met Asp Val Val Tyr Asn His Thr Phe Asn Val Gly Val Ser Asp Phe 
                485                 490                 495     


Asp Lys Ile Val Pro Gln Tyr Tyr Tyr Arg Thr Asp Ser Ala Gly Asn 
            500                 505                 510         


Tyr Thr Asn Gly Ser Gly Val Gly Asn Glu Ile Ala Thr Glu Arg Pro 
        515                 520                 525             


Met Val Gln Lys Phe Val Leu Asp Ser Val Lys Tyr Trp Val Lys Glu 
    530                 535                 540                 


Tyr His Ile Asp Gly Phe Arg Phe Asp Leu Met Ala Leu Leu Gly Lys 
545                 550                 555                 560 


Asp Thr Met Ala Lys Ile Ser Lys Glu Leu His Ala Ile Asn Pro Gly 
                565                 570                 575     


Ile Val Leu Tyr Gly Glu Pro Trp Thr Gly Gly Thr Ser Gly Leu Ser 
            580                 585                 590         


Ser Asp Gln Leu Val Thr Lys Gly Gln Gln Lys Gly Leu Gly Ile Gly 
        595                 600                 605             


Val Phe Asn Asp Asn Leu Arg Asn Ala Leu Asp Gly Asn Val Phe Asp 
    610                 615                 620                 


Ser Ser Ala Gln Gly Phe Ala Thr Gly Ala Thr Gly Leu Thr Asp Ala 
625                 630                 635                 640 


Ile Lys Asn Gly Val Glu Gly Ser Ile Asn Asp Phe Thr Ser Ser Pro 
                645                 650                 655     


Gly Glu Thr Ile Asn Tyr Val Thr Ser His Asp Asn Tyr Thr Leu Trp 
            660                 665                 670         


Asp Lys Ile Ala Leu Ser Asn Pro Asn Asp Ser Glu Ala Asp Arg Ile 
        675                 680                 685             


Lys Met Asp Glu Leu Ala Gln Ala Val Val Met Thr Ser Gln Gly Val 
    690                 695                 700                 


Pro Phe Met Gln Gly Gly Glu Glu Met Leu Arg Thr Lys Gly Gly Asn 
705                 710                 715                 720 


Asp Asn Ser Tyr Asn Ala Gly Asp Thr Val Asn Glu Phe Asp Trp Ser 
                725                 730                 735     


Arg Lys Ala Gln Tyr Pro Asp Val Phe Asn Tyr Tyr Ser Gly Leu Ile 
            740                 745                 750         


His Leu Arg Leu Asp His Pro Ala Phe Arg Met Thr Thr Ala Asn Glu 
        755                 760                 765             


Ile Asn Ser His Leu Gln Phe Leu Asn Ser Pro Glu Asn Thr Val Ala 
    770                 775                 780                 


Tyr Glu Leu Thr Asp His Val Asn Lys Asp Lys Trp Gly Asn Ile Ile 
785                 790                 795                 800 


Val Val Tyr Asn Pro Asn Lys Thr Ala Ala Thr Ile Asn Leu Pro Ser 
                805                 810                 815     


Gly Lys Trp Ala Ile Asn Ala Thr Ser Gly Lys Val Gly Glu Ser Thr 
            820                 825                 830         


Leu Gly Gln Ala Glu Gly Ser Val Gln Val Pro Gly Ile Ser Met Met 
        835                 840                 845             


Ile Leu His Gln Glu Val Ser Pro Asp His Gly Lys Lys 
    850                 855                 860     


<210>  17
<211>  48
<212>  DNA
<213>  artificial sequence

<220>
<223>  FORWARD primer

<400>  17
ttgcttttag ttcatcgata gcatcagcag attctacctc gacagaag                    48


<210>  18
<211>  36
<212>  DNA
<213>  artificial sequence

<220>
<223>  REVERSE primer

<400>  18
ttattgatta acgcgtttac tttttaccgt ggtctg                                 36


