                         SEQUENCE LISTING

<110>  AMYRIS, INC.
 
<120>  METHODS FOR GENOMIC INTEGRATION

<130>  107345.00528

<150>  US 61/937,444
<151>  2014-02-07

<150>  US 61/918,625
<151>  2013-12-19

<160>  120   

<170>  PatentIn version 3.5

<210>  1
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Homing endonuclease conserved sequence motif

<400>  1

Leu Ala Gly Leu Ile Asp Ala Asp Gly 
1               5                   


<210>  2
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Homing endonuclease conserved sequence motif

<400>  2

Gly Ile Tyr Tyr Ile Gly 
1               5       


<210>  3
<211>  7804
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cas9 Genomic Expression Construct

<400>  3
gacggcacgg ccacgcgttt aaaccgccga ccagagattt tgcattccag tattcatcaa       60

tgatgaattc gtagacgcag atactgtaaa tgccgtgttc atcaagaaat gggcgcatta      120

ctacaagaag ttttgatatt ttttgtaact gtaatttcac tcatgcacaa gaaaaaaaaa      180

actggattaa aagggagccc aaggaaaact cctcagcata tatttagaag tctcctcagc      240

atatagttgt ttgttttctt tacacattca ctgtttaata aaacttttat aatatttcat      300

tatcggaact ctagattcta tacttgtttc ccaattgttg ctggtagtaa acgtatacgt      360

cataaaaggg aaaagccaca tgcggaagaa ttttatggaa aaaaaaaaaa cctcgaagtt      420

actacttcta gggggcctat caagtaaatt actcctggta cactgaagta tataagggat      480

atagaagcaa atagttgtca gtgcaatcct tcaagacgct cgtccaacgc cggcggacct      540

cgaatcctta catcacaccc aatcccccac aagtgatccc ccacacacca tagcttcaaa      600

atgtttctac tcctttttta ctcttccaga ttttctcgga ctccgcgcat cgccgtacca      660

cttcaaaaca cccaagcaca gcatactaaa tttcccctct ttcttcctct agggtgtcgt      720

taattacccg tactaaaggt ttggaaaaga aaaaagagac cgcctcgttt ctttttcttc      780

gtcgaaaaag gcaataaaaa tttttatcac gtttcttttt cttgaaaatt tttttttttg      840

atttttttct ctttcgatga cctcccattg atatttaagt taataaacgg tcttcaattt      900

ctcaagtttc agtttcattt ttcttgttct attacaactt tttttacttc ttgctcatta      960

gaaagaaagc atagcaaacc tcccgcgacc tccaaaatcg aactaccttc acaatggata     1020

agaaatactc tatcggtttg gatattggta ctaactccgt tggttgggcc gttatcactg     1080

atgaatacaa ggttccatct aagaagttca aagttttggg taacactgat agacactcta     1140

tcaagaagaa cttgattggt gctttgttat ttgactctgg tgaaaccgct gaggctaccc     1200

gtttaaaaag aactgctaga cgtagataca cccgtcgtaa aaacagaatc tgttatttgc     1260

aagagatctt ctccaacgaa atggctaagg ttgacgactc ttttttccat agattagaag     1320

aatctttctt agttgaagaa gataagaagc acgaacgtca tccaatcttc ggtaacattg     1380

tcgacgaagt tgcttaccat gaaaagtacc caactatcta tcacttgaga aagaaattgg     1440

ttgattctac tgacaaagcc gacttgagat tgatctactt ggctttagct catatgatca     1500

aattccgtgg tcatttttta attgaaggtg atttgaaccc agacaactct gacgttgata     1560

aattgttcat ccaattggtt caaacctata accaattgtt tgaagaaaac ccaattaacg     1620

cttctggtgt tgatgctaag gctatcttgt ctgctagatt gtctaaatct agaagattgg     1680

aaaacttaat tgctcaattg ccaggtgaaa aaaaaaacgg tttgttcggt aatttgattg     1740

ctttatcctt gggtttgacc ccaaatttca agtccaactt tgatttggct gaagatgcca     1800

agttgcaatt gtctaaggat acttacgatg atgatttaga taacttattg gctcaaattg     1860

gtgatcaata cgctgatttg tttttagctg ccaagaattt gtccgacgcc attttgttgt     1920

ctgacatctt gagagtcaac actgaaatta ccaaggcccc tttgtctgct tctatgatta     1980

agagatatga cgaacaccac caagacttga ccttgttgaa ggctttggtt agacaacaat     2040

tacctgaaaa gtataaggaa atttttttcg accaatctaa gaacggttac gctggttaca     2100

ttgacggtgg tgcctctcaa gaagaattct acaaattcat caaaccaatc ttggaaaaga     2160

tggacggtac tgaagaattg ttagttaaat tgaacagaga agacttgttg agaaaacaaa     2220

gaacctttga caacggttcc attcctcacc aaatccactt gggtgagtta cacgctattt     2280

tgagaagaca agaagatttc tacccattct taaaggacaa ccgtgaaaag attgaaaaga     2340

ttttgacctt cagaattcca tactacgtcg gtcctttggc tcgtggtaac tccagattcg     2400

cctggatgac tagaaagtcc gaagaaacta ttactccatg gaacttcgaa gaagtcgttg     2460

acaagggtgc ttctgctcaa tcctttatcg aaagaatgac caacttcgac aaaaacttgc     2520

caaacgaaaa agtcttgcca aagcactctt tgttgtatga atactttact gtttataatg     2580

aattgactaa agttaagtac gttactgaag gtatgagaaa accagctttt ttatctggtg     2640

aacaaaaaaa agctatcgtc gatttgttgt tcaaaactaa ccgtaaagtt accgtcaagc     2700

aattgaagga agattacttc aagaagattg aatgttttga ctccgtcgaa atctccggtg     2760

ttgaagacag attcaatgct tctttgggta cttaccacga cttgttgaaa attatcaagg     2820

acaaggattt cttagataac gaagaaaacg aagacatttt ggaagatatt gtcttgactt     2880

tgactttgtt cgaagataga gaaatgattg aagaaagatt gaagacttat gctcatttgt     2940

tcgacgataa ggtcatgaag caattaaaga gaagacgtta cactggttgg ggtagattgt     3000

ctagaaaatt gattaacggt atccgtgata aacaatctgg taagaccatc ttggatttct     3060

taaagtctga tggttttgcc aacagaaact tcatgcaatt gatccacgac gactctttga     3120

ctttcaagga ggacattcaa aaggctcaag tttctggtca aggtgactct ttgcatgaac     3180

acattgccaa cttggctggt tctccagcta ttaagaaggg tatcttgcaa actgttaagg     3240

ttgttgatga attagttaag gtcatgggta gacacaagcc agaaaacatc gtcatcgaaa     3300

tggctagaga aaaccaaact actcaaaagg gtcaaaagaa ttctagagaa agaatgaaga     3360

gaattgagga aggtattaag gaattaggtt cccaaatttt gaaggaacat ccagtcgaaa     3420

acactcaatt gcaaaacgaa aaattgtact tgtactactt acaaaacggt agagatatgt     3480

atgtcgacca agagttggac atcaacagat tgtccgacta cgatgttgat cacatcgttc     3540

cacaatcctt cttaaaggac gactctatcg acaacaaggt cttaaccaga tccgacaaaa     3600

acagaggtaa gtctgacaac gttccatccg aagaagttgt taaaaagatg aagaactact     3660

ggagacaatt gttgaacgcc aaattgatca ctcaaagaaa gttcgataat ttgaccaagg     3720

ctgaaagagg tggtttgtct gaattggata aggctggttt tattaaaaga caattggttg     3780

agactagaca aatcaccaag catgtcgctc aaattttaga ttccagaatg aacactaaat     3840

acgacgaaaa cgataagtta attagagaag ttaaggttat taccttgaag tctaagttgg     3900

tttctgattt cagaaaggac ttccaatttt acaaggtcag agaaattaac aactaccatc     3960

acgctcatga tgcttacttg aacgccgttg ttggtaccgc tttgattaaa aagtacccaa     4020

agttggaatc cgaatttgtc tacggtgact acaaggtcta cgatgtcaga aaaatgatcg     4080

ctaagtccga acaagagatt ggtaaggcta ctgccaagta cttcttttac tctaacatca     4140

tgaacttttt caagactgaa atcactttag ctaacggtga aattcgtaag agaccattga     4200

ttgaaaccaa cggtgagact ggtgaaatcg tttgggataa gggtcgtgat ttcgctactg     4260

ttagaaaggt cttatctatg ccacaagtta acatcgtcaa gaaaaccgaa gttcaaactg     4320

gtggtttttc taaggaatct atcttgccaa aaagaaactc tgataaattg attgctagaa     4380

agaaggattg ggacccaaag aagtacggtg gtttcgattc cccaaccgtc gcttactccg     4440

tcttggttgt cgctaaagtt gaaaagggta agtccaagaa attgaagtct gttaaggaat     4500

tgttgggtat cactatcatg gaaagatctt ccttcgaaaa gaacccaatc gattttttag     4560

aggccaaggg ttataaggaa gttaaaaagg acttaattat taagttgcca aagtactctt     4620

tgttcgaatt agaaaacggt agaaaaagaa tgttggcctc tgctggtgag ttgcaaaaag     4680

gtaacgaatt ggccttgcca tctaagtatg ttaacttttt gtacttggcc tctcattacg     4740

agaagttgaa gggttcccca gaagataacg aacaaaagca attgttcgtc gaacaacaca     4800

aacattactt ggatgaaatt atcgaacaaa tctccgagtt ttccaaacgt gttatcttgg     4860

ctgacgccaa tttggataag gttttgtctg cttataataa gcatagagat aagccaatta     4920

gagaacaagc cgagaacatc attcacttgt tcactttgac taatttaggt gctccagctg     4980

ccttcaaata tttcgacacc accattgata gaaagagata cacctccact aaggaagtct     5040

tggatgccac cttgattcac caatctatca ctggtttgta cgaaactaga atcgatttgt     5100

ctcaattagg tggtgattcc cgtgccgacc caaagaagaa gagaaaggtc taaacaggcc     5160

ccttttcctt tgtcgatatc atgtaattag ttatgtcacg cttacattca cgccctcccc     5220

ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt     5280

atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt tttctttttt     5340

ttctgtacaa acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt gagaaggttt     5400

tggcatcccc gcgtgcttgg ccggccgttt aatcagcgcc cagagactag cactgaatga     5460

tcaacgggta gttcacacga tgcacgagcg caacgctcac aatgacagtc tggacatcaa     5520

tagtcacact acagaaggtg atctctcaac ttcagcagac catagcgtgt aataaatgca     5580

taattatttt tctctaaaaa aaactcagct gaaattttat ataagtacta cattttatat     5640

acatattaca tactgaacaa taagcgcgtt tgacatttta attttcgaag accgcgaatc     5700

cttacatcac acccagtccc ccaatagttc ccccacacac catgcttcaa aaacgcactg     5760

tactcctttt tactcttccg gattttctcg gactctccgc atcgccgcac gagccaagcc     5820

acacccacac acctcatacc atgtttcccc tctttgactc tttcgtgcgg ctccattacc     5880

cgcatgaaac tgtataaaag taacaaaaga ctatttcgtt tctttttctt tgtcggaaaa     5940

ggcaaaaaaa aaaattttta tcacatttct ttttcttgaa aatttttttt gggatttttt     6000

ctctttcgat gacctcccat tgatatttaa gttaataaaa ggtctcccgt tttccaagtt     6060

ttaatttgtt cctcttgttt agtcattctt cttctcagca ttggtcaatt agaaagagag     6120

catagcaaac tgatctaagt tttaattacc atatgaaaaa gcctgaactc accgcgacgt     6180

ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg     6240

agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg     6300

taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg     6360

ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt     6420

gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg     6480

ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga     6540

cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt     6600

tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg     6660

tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg     6720

aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc     6780

gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg     6840

ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg     6900

agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg     6960

gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc     7020

agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg     7080

cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa     7140

accgacgccc cagcactcgt ccgagggcaa aggaataggg aaattgataa gacttttcta     7200

gttgcatatc ttttatattt aaatcttatc tattagttaa ttttttgtaa tttatcctta     7260

tatagtctgg ttattctaaa atatcatttc agtatctaaa atagttcttt ttttttttga     7320

gttagatttt tatgggggag agttgaagtg ttgaattttc ccactttgct tcgggattgt     7380

gggtcattct gtcgataact gatatcacat catcaataga acctcttaga tgcacgagcg     7440

caacgctcac aattaatcag cgcccagaga ctagcactga atgatcaacg ggtagttcac     7500

acaggtccgc cggcgttgga cgagcgtcca gccagtaaaa tccatactca acgacgatat     7560

gaacaaattt ccctcattcc gatgctgtat atgtgtataa atttttacat gctcttctgt     7620

ttagacacag aacagcttta aataaaatgt tggatatact ttttctgcct gtggtgtcat     7680

ccacgctttt aattcatctc ttgtatggtt gacaatttgg ctatttttta acagaaccca     7740

acggtaattg aaattaaaag ggaaacgagt gggggcggcg gtttaaacgc gtggccgtgc     7800

cgtc                                                                  7804


<210>  4
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RHR2 genomic target Sequence

<400>  4
acctctggta cccgtgaca                                                    19


<210>  5
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: HO genomic target Sequence

<400>  5
ccggcttgat cgactcaga                                                    19


<210>  6
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: ADH5 genomic target sequence

<400>  6
gggtcattgg tatcgatgg                                                    19


<210>  7
<211>  1026
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: gRNA construct targeting RHR2 locus

<400>  7
ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag       60

agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc      120

gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa      180

gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagcctctt      240

tgaaaagata atgtatgatt atgctttcac tcatatttat acagaaactt gatgttttct      300

ttcgagtata tacaaggtga ttacatgtac gtttgaagta caactctaga ttttgtagtg      360

ccctcttggg ctagcggtaa aggtgcgcat tttttcacac cctacaatgt tctgttcaaa      420

agattttggt caaacgctgt agaagtgaaa gttggtgcgc atgtttcggc gttcgaaact      480

tctccgcagt gaaagataaa tgatcgccgc caatgatgtc aagcagtttt agagctagaa      540

atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg      600

gtgctttttt tgttttttat gtctcagctt ttgttccctt tagtgagggt taattgcgcg      660

cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc      720

acacaacata ggagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgaggta      780

actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca      840

gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc      900

cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc      960

tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat     1020

gtgagc                                                                1026


<210>  8
<211>  1080
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: gRNA construct targeting HO locus

<400>  8
gacggcacgg ccacgcgttt aaaccgcctt ataaatcaaa agaatagacc gagatagggt       60

tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca      120

aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa      180

gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat      240

ttagagcttg acggggaaag cctctttgaa aagataatgt atgattatgc tttcactcat      300

atttatacag aaacttgatg ttttctttcg agtatataca aggtgattac atgtacgttt      360

gaagtacaac tctagatttt gtagtgccct cttgggctag cggtaaaggt gcgcattttt      420

tcacacccta caatgttctg ttcaaaagat tttggtcaaa cgctgtagaa gtgaaagttg      480

gtgcgcatgt ttcggcgttc gaaacttctc cgcagtgaaa gataaatgat cgccggcttg      540

atcgactcag agttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa      600

cttgaaaaag tggcaccgag tcggtggtgc tttttttgtt ttttatgtct cagcttttgt      660

tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg      720

tgaaattgtt atccgctcac aattccacac aacataggag ccggaagcat aaagtgtaaa      780

gcctggggtg cctaatgagt gaggtaactc acattaattg cgttgcgctc actgcccgct      840

ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga      900

ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc      960

gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa     1020

tcaggggata acgcaggaaa gaacatgtga gccggtgttt aaaccccagc gcctggcggg     1080


<210>  9
<211>  1080
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: gRNA construct targeting ADH5 locus

<400>  9
gacggcacgg ccacgcgttt aaaccgcctt ataaatcaaa agaatagacc gagatagggt       60

tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca      120

aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa      180

gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat      240

ttagagcttg acggggaaag cctctttgaa aagataatgt atgattatgc tttcactcat      300

atttatacag aaacttgatg ttttctttcg agtatataca aggtgattac atgtacgttt      360

gaagtacaac tctagatttt gtagtgccct cttgggctag cggtaaaggt gcgcattttt      420

tcacacccta caatgttctg ttcaaaagat tttggtcaaa cgctgtagaa gtgaaagttg      480

gtgcgcatgt ttcggcgttc gaaacttctc cgcagtgaaa gataaatgat cggggtcatt      540

ggtatcgatg ggttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa      600

cttgaaaaag tggcaccgag tcggtggtgc tttttttgtt ttttatgtct cagcttttgt      660

tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg      720

tgaaattgtt atccgctcac aattccacac aacataggag ccggaagcat aaagtgtaaa      780

gcctggggtg cctaatgagt gaggtaactc acattaattg cgttgcgctc actgcccgct      840

ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga      900

ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc      960

gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa     1020

tcaggggata acgcaggaaa gaacatgtga gccggtgttt aaaccccagc gcctggcggg     1080


<210>  10
<211>  5994
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: pRS4XX vector backbone

<400>  10
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

aaatacacat catcgtccta caagttcatc aaagtgttgg acagacaact ataccagcat      240

ggatctcttg tatcggttct tttctcccgc tctctcgcaa taacaatgaa cactgggtca      300

atcatagcct acacaggtga acagagtagc gtttatacag ggtttatacg gtgattccta      360

cggcaaaaat ttttcatttc taaaaaaaaa aagaaaaatt tttctttcca acgctagaag      420

gaaaagaaaa atctaattaa attgatttgg tgattttctg agagttccct ttttcatata      480

tcgaattttg aatataaaag gagatcgaaa aaatttttct attcaatctg ttttctggtt      540

ttatttgata gtttttttgt gtattattat tatggattag tactggttta tatgggtttt      600

tctgtataac ttctttttat tttagtttgt ttaatcttat tttgagttac attatagttc      660

cctaactgca agagaagtaa cattaaaaat gaccactctt gacgacacgg cttaccggta      720

ccgcaccagt gtcccggggg acgccgaggc catcgaggca ctggatgggt ccttcaccac      780

cgacaccgtc ttccgcgtca ccgccaccgg ggacggcttc accctgcggg aggtgccggt      840

ggacccgccc ctgaccaagg tgttccccga cgacgaatcg gacgacgaat cggacgccgg      900

ggaggacggc gacccggact cccggacgtt cgtcgcgtac ggggacgacg gcgacctggc      960

gggcttcgtg gtcgtctcgt actccggctg gaaccgccgg ctgaccgtcg aggacatcga     1020

ggtcgccccg gagcaccggg ggcacggggt cgggcgcgcg ttgatggggc tcgcgacgga     1080

gttcgcccgc gagcggggcg ccgggcacct ctggctggag gtcaccaacg tcaacgcacc     1140

ggcgatccac gcgtaccggc ggatggggtt caccctctgc ggcctggaca ccgccctgta     1200

cgacggcacc gcctcggacg gcgagcaggc gctctacatg agcatgccct gcccctgagt     1260

ttaacttgat actactagat tttttctctt catttataaa atttttggtt ataattgaag     1320

ctttagaagt atgaaaaaat cctttttttt cattctttgc aaccaaaata agaagcttct     1380

tttattcatt gaaatgatga atataaacct aacaaaagaa aaagactcga atatcaaaca     1440

ttaaaaaaaa ataaaagagg ttatctgttt tcccatttag ttggagtttg cattttctaa     1500

tagatagaac tctcaattaa tgtggattta gtttctctgt tcgttttttt ttgttttgtt     1560

ctcactgtat ttacatttct atttagtatt tagttattca tataatctta acttgcggtg     1620

tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt aagcgttaat     1680

attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc     1740

gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt     1800

ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa     1860

accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg     1920

tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga     1980

cggggaaagc ctctttgaaa agataatgta tgattatgct ttcactcata tttatacaga     2040

aacttgatgt tttctttcga gtatatacaa ggtgattaca tgtacgtttg aagtacaact     2100

ctagattttg tagtgccctc ttgggctagc ggtaaaggtg cgcatttttt cacaccctac     2160

aatgttctgt tcaaaagatt ttggtcaaac gctgtagaag tgaaagttgg tgcgcatgtt     2220

tcggcgttcg aaacttctcc gcagtgaaag ataaatgatc gatacgttct ctatggagga     2280

gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt     2340

ggcaccgagt cggtggtgct ttttttgttt tttatgtctc agcttttgtt ccctttagtg     2400

agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta     2460

tccgctcaca attccacaca acataggagc cggaagcata aagtgtaaag cctggggtgc     2520

ctaatgagtg aggtaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg     2580

aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg     2640

tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg     2700

gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa     2760

cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc     2820

gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc     2880

aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag     2940

ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct     3000

cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta     3060

ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc     3120

cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc     3180

agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt     3240

gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct     3300

gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc     3360

tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca     3420

agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta     3480

agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa     3540

atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg     3600

cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg     3660

actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc     3720

aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc     3780

cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa     3840

ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc     3900

cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg     3960

ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc     4020

cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat     4080

ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg     4140

tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc     4200

ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg     4260

aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat     4320

gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg     4380

gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg     4440

ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct     4500

catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac     4560

atttccccga aaagtgccac ctgaacgaag catctgtgct tcattttgta gaacaaaaat     4620

gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga     4680

aatgcaacgc gaaagcgcta ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca     4740

aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga     4800

acagaaatgc aacgcgagag cgctatttta ccaacaaaga atctatactt cttttttgtt     4860

ctacaaaaat gcatcccgag agcgctattt ttctaacaaa gcatcttaga ttactttttt     4920

tctcctttgt gcgctctata atgcagtctc ttgataactt tttgcactgt aggtccgtta     4980

aggttagaag aaggctactt tggtgtctat tttctcttcc ataaaaaaag cctgactcca     5040

cttcccgcgt ttactgatta ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat     5100

ccccgattat attctatacc gatgtggatt gcgcatactt tgtgaacaga aagtgatagc     5160

gttgatgatt cttcattggt cagaaaatta tgaacggttt cttctatttt gtctctatat     5220

actacgtata ggaaatgttt acattttcgt attgttttcg attcactcta tgaatagttc     5280

ttactacaat ttttttgtct aaagagtaat actagagata aacataaaaa atgtagaggt     5340

cgagtttaga tgcaagttca aggagcgaaa ggtggatggg taggttatat agggatatag     5400

cacagagata tatagcaaag agatactttt gagcaatgtt tgtggaagcg gtattcgcaa     5460

tattttagta gctcgttaca gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga     5520

gcgcttttgg ttttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc     5580

ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg     5640

cgcacataca gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg tatatatata     5700

tacatgagaa gaacggcata gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat     5760

ttatgtagga tgaaaggtag tctagtacct cctgtgatat tatcccattc catgcggggt     5820

atcgtatgct tccttcagca ctacccttta gctgttctat atgctgccac tcctcaattg     5880

gattagtctc atccttcaat gctatcattt cctttgatat tggatcatat taagaaacca     5940

ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc           5994


<210>  11
<211>  6039
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: pAM1114 - closed plasmid bearing a NatA 
       (Nourseothricin acetyltransferase from Streptomyces noursei) 
       selectable marker

<400>  11
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

aaatacacat catcgtccta caagttcatc aaagtgttgg acagacaact ataccagcat      240

ggatctcttg tatcggttct tttctcccgc tctctcgcaa taacaatgaa cactgggtca      300

atcatagcct acacaggtga acagagtagc gtttatacag ggtttatacg gtgattccta      360

cggcaaaaat ttttcatttc taaaaaaaaa aagaaaaatt tttctttcca acgctagaag      420

gaaaagaaaa atctaattaa attgatttgg tgattttctg agagttccct ttttcatata      480

tcgaattttg aatataaaag gagatcgaaa aaatttttct attcaatctg ttttctggtt      540

ttatttgata gtttttttgt gtattattat tatggattag tactggttta tatgggtttt      600

tctgtataac ttctttttat tttagtttgt ttaatcttat tttgagttac attatagttc      660

cctaactgca agagaagtaa cattaaaaat gaccactctt gacgacacgg cttaccggta      720

ccgcaccagt gtcccggggg acgccgaggc catcgaggca ctggatgggt ccttcaccac      780

cgacaccgtc ttccgcgtca ccgccaccgg ggacggcttc accctgcggg aggtgccggt      840

ggacccgccc ctgaccaagg tgttccccga cgacgaatcg gacgacgaat cggacgccgg      900

ggaggacggc gacccggact cccggacgtt cgtcgcgtac ggggacgacg gcgacctggc      960

gggcttcgtg gtcgtctcgt actccggctg gaaccgccgg ctgaccgtcg aggacatcga     1020

ggtcgccccg gagcaccggg ggcacggggt cgggcgcgcg ttgatggggc tcgcgacgga     1080

gttcgcccgc gagcggggcg ccgggcacct ctggctggag gtcaccaacg tcaacgcacc     1140

ggcgatccac gcgtaccggc ggatggggtt caccctctgc ggcctggaca ccgccctgta     1200

cgacggcacc gcctcggacg gcgagcaggc gctctacatg agcatgccct gcccctgagt     1260

ttaacttgat actactagat tttttctctt catttataaa atttttggtt ataattgaag     1320

ctttagaagt atgaaaaaat cctttttttt cattctttgc aaccaaaata agaagcttct     1380

tttattcatt gaaatgatga atataaacct aacaaaagaa aaagactcga atatcaaaca     1440

ttaaaaaaaa ataaaagagg ttatctgttt tcccatttag ttggagtttg cattttctaa     1500

tagatagaac tctcaattaa tgtggattta gtttctctgt tcgttttttt ttgttttgtt     1560

ctcactgtat ttacatttct atttagtatt tagttattca tataatctta acttgcggtg     1620

tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt aagcgttaat     1680

attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc     1740

gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt     1800

ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa     1860

accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg     1920

tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga     1980

cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct     2040

agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat     2100

gcgccgctac agggcgcgtc gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg     2160

cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg     2220

cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt     2280

gagcgcgcgt aatacgactc actatagggc gaattgggta ccgggccccc cctcgaggtc     2340

gacggtatcg ataagcttga tatcgaattc ctgcagcccg ggggatccac tagttctaga     2400

gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattgcgcg     2460

cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc     2520

acacaacata ggagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgaggta     2580

actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca     2640

gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc     2700

cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc     2760

tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat     2820

gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt     2880

ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg     2940

aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc     3000

tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt     3060

ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa     3120

gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta     3180

tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa     3240

caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa     3300

ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt     3360

cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt     3420

ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat     3480

cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat     3540

gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc     3600

aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc     3660

acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta     3720

gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga     3780

cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg     3840

cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc     3900

tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat     3960

cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag     4020

gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat     4080

cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa     4140

ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa     4200

gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga     4260

taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg     4320

gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc     4380

acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg     4440

aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact     4500

cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat     4560

atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt     4620

gccacctgaa cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc     4680

taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgaaag     4740

cgctatttta ccaacgaaga atctgtgctt catttttgta aaacaaaaat gcaacgcgag     4800

agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc     4860

gagagcgcta ttttaccaac aaagaatcta tacttctttt ttgttctaca aaaatgcatc     4920

ccgagagcgc tatttttcta acaaagcatc ttagattact ttttttctcc tttgtgcgct     4980

ctataatgca gtctcttgat aactttttgc actgtaggtc cgttaaggtt agaagaaggc     5040

tactttggtg tctattttct cttccataaa aaaagcctga ctccacttcc cgcgtttact     5100

gattactagc gaagctgcgg gtgcattttt tcaagataaa ggcatccccg attatattct     5160

ataccgatgt ggattgcgca tactttgtga acagaaagtg atagcgttga tgattcttca     5220

ttggtcagaa aattatgaac ggtttcttct attttgtctc tatatactac gtataggaaa     5280

tgtttacatt ttcgtattgt tttcgattca ctctatgaat agttcttact acaatttttt     5340

tgtctaaaga gtaatactag agataaacat aaaaaatgta gaggtcgagt ttagatgcaa     5400

gttcaaggag cgaaaggtgg atgggtaggt tatataggga tatagcacag agatatatag     5460

caaagagata cttttgagca atgtttgtgg aagcggtatt cgcaatattt tagtagctcg     5520

ttacagtccg gtgcgttttt ggttttttga aagtgcgtct tcagagcgct tttggttttc     5580

aaaagcgctc tgaagttcct atactttcta gagaatagga acttcggaat aggaacttca     5640

aagcgtttcc gaaaacgagc gcttccgaaa atgcaacgcg agctgcgcac atacagctca     5700

ctgttcacgt cgcacctata tctgcgtgtt gcctgtatat atatatacat gagaagaacg     5760

gcatagtgcg tgtttatgct taaatgcgta cttatatgcg tctatttatg taggatgaaa     5820

ggtagtctag tacctcctgt gatattatcc cattccatgc ggggtatcgt atgcttcctt     5880

cagcactacc ctttagctgt tctatatgct gccactcctc aattggatta gtctcatcct     5940

tcaatgctat catttccttt gatattggat catactaaga aaccattatt atcatgacat     6000

taacctataa aaataggcgt atcacgaggc cctttcgtc                            6039


<210>  12
<211>  5662
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: pAM1114 vector linearized using DRPE308/309 (the 
       linear cassette was co-transformed with vector linearized by PCR 
       inside of the NatA marker)

<400>  12
atggggttca ccctctgcgg cctggacacc gccctgtacg acggcaccgc ctcggacggc       60

gagcaggcgc tctacatgag catgccctgc ccctgagttt aacttgatac tactagattt      120

tttctcttca tttataaaat ttttggttat aattgaagct ttagaagtat gaaaaaatcc      180

ttttttttca ttctttgcaa ccaaaataag aagcttcttt tattcattga aatgatgaat      240

ataaacctaa caaaagaaaa agactcgaat atcaaacatt aaaaaaaaat aaaagaggtt      300

atctgttttc ccatttagtt ggagtttgca ttttctaata gatagaactc tcaattaatg      360

tggatttagt ttctctgttc gttttttttt gttttgttct cactgtattt acatttctat      420

ttagtattta gttattcata taatcttaac ttgcggtgtg aaataccgca cagatgcgta      480

aggagaaaat accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa      540

atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata      600

aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac      660

tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc      720

cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa      780

atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg      840

cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg      900

tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcgc      960

gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc     1020

tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag     1080

ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac     1140

tatagggcga attgggtacc gggccccccc tcgaggtcga cggtatcgat aagcttgata     1200

tcgaattcct gcagcccggg ggatccacta gttctagagc ggccgccacc gcggtggagc     1260

tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag     1320

ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatagg agccggaagc     1380

ataaagtgta aagcctgggg tgcctaatga gtgaggtaac tcacattaat tgcgttgcgc     1440

tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa     1500

cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg     1560

ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg     1620

ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag     1680

gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac     1740

gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga     1800

taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt     1860

accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc     1920

tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc     1980

cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta     2040

agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat     2100

gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca     2160

gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct     2220

tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt     2280

acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct     2340

cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc     2400

acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa     2460

acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta     2520

tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc     2580

ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat     2640

ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta     2700

tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt     2760

aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt     2820

ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg     2880

ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc     2940

gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc     3000

gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg     3060

cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga     3120

actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta     3180

ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct     3240

tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag     3300

ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga     3360

agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat     3420

aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgaacg aagcatctgt     3480

gcttcatttt gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct     3540

gagctgcatt tttacagaac agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat     3600

ctgtgcttca tttttgtaaa acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag     3660

aatctgagct gcatttttac agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa     3720

agaatctata cttctttttt gttctacaaa aatgcatccc gagagcgcta tttttctaac     3780

aaagcatctt agattacttt ttttctcctt tgtgcgctct ataatgcagt ctcttgataa     3840

ctttttgcac tgtaggtccg ttaaggttag aagaaggcta ctttggtgtc tattttctct     3900

tccataaaaa aagcctgact ccacttcccg cgtttactga ttactagcga agctgcgggt     3960

gcattttttc aagataaagg catccccgat tatattctat accgatgtgg attgcgcata     4020

ctttgtgaac agaaagtgat agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg     4080

tttcttctat tttgtctcta tatactacgt ataggaaatg tttacatttt cgtattgttt     4140

tcgattcact ctatgaatag ttcttactac aatttttttg tctaaagagt aatactagag     4200

ataaacataa aaaatgtaga ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat     4260

gggtaggtta tatagggata tagcacagag atatatagca aagagatact tttgagcaat     4320

gtttgtggaa gcggtattcg caatatttta gtagctcgtt acagtccggt gcgtttttgg     4380

ttttttgaaa gtgcgtcttc agagcgcttt tggttttcaa aagcgctctg aagttcctat     4440

actttctaga gaataggaac ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc     4500

ttccgaaaat gcaacgcgag ctgcgcacat acagctcact gttcacgtcg cacctatatc     4560

tgcgtgttgc ctgtatatat atatacatga gaagaacggc atagtgcgtg tttatgctta     4620

aatgcgtact tatatgcgtc tatttatgta ggatgaaagg tagtctagta cctcctgtga     4680

tattatccca ttccatgcgg ggtatcgtat gcttccttca gcactaccct ttagctgttc     4740

tatatgctgc cactcctcaa ttggattagt ctcatccttc aatgctatca tttcctttga     4800

tattggatca tactaagaaa ccattattat catgacatta acctataaaa ataggcgtat     4860

cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca     4920

gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca     4980

gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca     5040

gattgtactg agagtgcaaa tacacatcat cgtcctacaa gttcatcaaa gtgttggaca     5100

gacaactata ccagcatgga tctcttgtat cggttctttt ctcccgctct ctcgcaataa     5160

caatgaacac tgggtcaatc atagcctaca caggtgaaca gagtagcgtt tatacagggt     5220

ttatacggtg attcctacgg caaaaatttt tcatttctaa aaaaaaaaag aaaaattttt     5280

ctttccaacg ctagaaggaa aagaaaaatc taattaaatt gatttggtga ttttctgaga     5340

gttccctttt tcatatatcg aattttgaat ataaaaggag atcgaaaaaa tttttctatt     5400

caatctgttt tctggtttta tttgatagtt tttttgtgta ttattattat ggattagtac     5460

tggtttatat gggtttttct gtataacttc tttttatttt agtttgttta atcttatttt     5520

gagttacatt atagttccct aactgcaaga gaagtaacat taaaaatgac cactcttgac     5580

gacacggctt accggtaccg caccagtgtc ccgggggacg ccgaggccat cgaggcactg     5640

gatgggtcct tcaccaccga ca                                              5662


<210>  13
<211>  651
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: DRPE310/311 PCR fragment from pAM1114 (a complementary
       overlapping NatA ORF fragment)

<400>  13
cttattttga gttacattat agttccctaa ctgcaagaga agtaacatta aaaatgacca       60

ctcttgacga cacggcttac cggtaccgca ccagtgtccc gggggacgcc gaggccatcg      120

aggcactgga tgggtccttc accaccgaca ccgtcttccg cgtcaccgcc accggggacg      180

gcttcaccct gcgggaggtg ccggtggacc cgcccctgac caaggtgttc cccgacgacg      240

aatcggacga cgaatcggac gccggggagg acggcgaccc ggactcccgg acgttcgtcg      300

cgtacgggga cgacggcgac ctggcgggct tcgtggtcgt ctcgtactcc ggctggaacc      360

gccggctgac cgtcgaggac atcgaggtcg ccccggagca ccgggggcac ggggtcgggc      420

gcgcgttgat ggggctcgcg acggagttcg cccgcgagcg gggcgccggg cacctctggc      480

tggaggtcac caacgtcaac gcaccggcga tccacgcgta ccggcggatg gggttcaccc      540

tctgcggcct ggacaccgcc ctgtacgacg gcaccgcctc ggacggcgag caggcgctct      600

acatgagcat gccctgcccc tgagtttaac ttgatactac tagatttttt c               651


<210>  14
<211>  1024
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Donor DNA  for integration at RHR2 locus

<400>  14
ggaaatccgt atcattttct cgcatacacg aacccgcgtg cgcctggtaa attgcaggat       60

tctcattgtc cggttttctt tatgggaata atcatcatca ccattatcac tgttactctt      120

gcgatcatca tcaataacat aattttttta acgctgtttg atgatggtat gtgcttttgt      180

tgttccttac tcaccttttc ctttgtgtct tttaattttg accattttga ccattttgac      240

ctttgatgat gtgtgagttc ctcttttctt tttttctttt cttttttcct ttttttttct      300

tttcttactc tgttaatcac tttctttcct ttttcttcat attgtcgtct tgttcatttt      360

cgttcaattg ataatgtata taaatctttc ctaagtatct cttgattgcc atttttttct      420

ttccaagttt ccttgttatg aaacgtttca atgttttaaa atatatcaga acaacaaaag      480

caaatataca aaccatcgca cgctcgtcca acgccggcgg acctttttct tttatttttt      540

tgataaaact actacgctaa aaataaaata aaaatgtatg atttccctcc atttccgacc      600

aattgtataa ttttatatct gcatgactta ataatataat ataatactta taaaatacga      660

atagaaaaat ttaaaccgat gtaatgcatc cttttctttg ttgtcttcgg atgatctgcc      720

gtgacaggtg gttcgcgcaa atcaaactgg attagagaat ttaacacaga aataaaaaag      780

gaagattcaa tcttcgtttt tgttttatat cttactataa aagtgttttt ttttagtacg      840

acgagaagtt agagtaatta taaaaggaat gcttatttaa atttatttct tagacttctt      900

ttcagacttc ttagcagcct cagtttgttc cttaacgacc ttcttaacaa tcttttgttc      960

ctcaatcaag aaagctctga cgattctttc cttgacacag ttggcacatc tggaaccacc     1020

gtaa                                                                  1024


<210>  15
<211>  1024
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Donor DNA  for integration at HO locus

<400>  15
cgcaagtcct gtttctatgc ctttctctta gtaattcacg aaataaacct atggtttacg       60

aaatgatcca cgaaaatcat gttattattt acatcaacat atcgcgaaaa ttcatgtcat      120

gtccacatta acatcattgc agagcaacaa ttcattttca tagagaaatt tgctactatc      180

acccactagt actaccattg gtacctacta ctttgaattg tactaccgct gggcgttatt      240

aggtgtgaaa ccacgaaaag ttcaccataa cttcgaataa agtcgcggaa aaaagtaaac      300

agctattgct actcaaatga ggtttgcaga agcttgttga agcatgatga agcgttctaa      360

acgcactatt catcattaaa tatttaaagc tcataaaatt gtattcaatt cctattctaa      420

atggctttta tttctattac aactattagc tctaaatcca tatcctcata agcagcaatc      480

aattctatct atactttaaa cgctcgtcca acgccggcgg acctaatgtg tatattagtt      540

taaaaagttg tatgtaataa aagtaaaatt taatattttg gatgaaaaaa accattttta      600

gactttttct taactagaat gctggagtag aaatacgcca tctcaagata caaaaagcgt      660

taccggcact gatttgtttc aaccagtata tagattatta ttgggtcttg atcaactttc      720

ctcagacata tcagtaacag ttatcaagct aaatatttac gcgaaagaaa aacaaatatt      780

ttaattgtga tacttgtgaa ttttatttta ttaaggatac aaagttaaga gaaaacaaaa      840

tttatataca atataagtaa tattcatata tatgtgatga atgcagtctt aacgagaaga      900

catggccttg gtgacaactc tcttcaaacc aacttcagcc tttctcaatt catcagcaga      960

tgggtcttcg atttgcaaag cagccaaagc atcggacaaa gcagcttcaa tcttggactt     1020

ggaa                                                                  1024


<210>  16
<211>  1024
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Donor DNA  for integration at ADH5 locus

<400>  16
taattttaca agtagtgtct tcatgacgga ttcatagtct atccaagcgt ttgcccaaaa       60

ttttgcagta aatttaatgt tttctgtata ggataatggt gcgccattca agtcccgcga      120

aaaatgactg atgtctacag gacaggggcg caatatatgt tctctgacat tgcacctttt      180

gaatatatca tgtgtttgtt ctgctatctg cttgtagaag ggtacgctaa cagagccggc      240

ggcattgtaa aaagttctcc tttcgcggaa ggatgagtca aaaagcatgt gacaatgaaa      300

taatcaaatt gtgacatctg ctgacgcggg atcgttcctt cgtattgtct agattgtaat      360

ctatataaca tactacgaat ataaaagagg gactacaaga tatttctagc gcaaactact      420

gctttactgt ctcacaatgt ctctgattgg aagataccta agaaaattat ttaactacat      480

atctacaaaa tcaaagcatc cgctcgtcca acgccggcgg accttctttt gtaacgaatt      540

tgatgaatat atttttactt tttatataag ctattttgta gatattgact ttttacgatt      600

tatttgtaac aatgagaatt actccatttc tgaacttcag taaatagcga gtgattctgt      660

actttgcgag aaccggtgga catttggtat tttgccttac aagaacaacc tatacaaacg      720

tttcaatatc taattctttg taatccattg ttttacgaga catataatgt gatatataga      780

tgaactttac gtataaaatg atatatttaa aactagcaac tgcgtgcgta agacaaactg      840

aaataggcca tttacggaaa agaaatttaa taatgtcgac tggaaactga aaccaggagg      900

agtagaaatt ggttaaattg attagctaaa atttactcgt tgtggacaga gtttgagcca      960

agcggaatgt tttcaaggct ttctttgttt cgaagggcag ctctggctcc tgcccctatg     1020

agaa                                                                  1024


<210>  17
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RHR2 upstream primer

<400>  17
gggtgcgaag taccaccacg tttctttttc atctct                                 36


<210>  18
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: HO upstream primer

<400>  18
acgtgtgtgt ctcatggaaa ttgatgcagt tgaagaca                               38


<210>  19
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: ADH5 upstream primer

<400>  19
ggcgttatat ccaaacattt cagacagaag att                                    33


<210>  20
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Primer for deletion linker

<400>  20
aggtccgccg gcgttggacg agcg                                              24


<210>  21
<211>  1078
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Donor DNA for integration at Gal80 locus

<400>  21
gacggcacgg ccacgcgttt aaaccgccca gatggaatcc cttccataga gagaaggagc       60

aagcaactga cccaatattg actgccactg gacctgaaga catgcaacaa agtgcaagca      120

tagtggggcc ttcttccaat gctaatccgg tcactgccac tgctgctacg gaaaaccaac      180

ctaaaggtat taacttcttc actataagaa aatcacacga gcgcccggac gatgtctctg      240

tttaaatggc gcaagttttc cgctttgtaa tatatattta tacccctttc ttctctcccc      300

tgcaatataa tagtttaatt ctaatattaa taatatccta tattttcttc atttaccggc      360

gcactctcgc ccgaacgacc tcaaaatgtc tgctacattc ataataacca aaagctcata      420

actttttttt ttgaacctga atatatatac atcacatgtc actgctggtc cttgccgacc      480

agcgtataca atctcgatag ttggtttccc gttctttcca ctcccgtccg ctcgtccaac      540

gccggcggac ctaagcatct tgccctgtgc ttggccccca gtgcagcgaa cgttataaaa      600

acgaatactg agtatatatc tatgtaaaac aaccatatca tttcttgttc tgaactttgt      660

ttacctaact agttttaaat ttcccttttt cgtgcatgcg ggtgttctta tttattagca      720

tactacattt gaaatatcaa atttccttag tagaaaagtg agagaaggtg cactgacaca      780

aaaaataaaa tgctacgtat aactgtcaaa actttgcagc agcgggcatc cttccatcat      840

agcttcaaac atattagcgt tcctgatctt catacccgtg ctcaaaatga tcaaacaaac      900

tgttattgcc aagaaataaa cgcaaggctg ccttcaaaaa ctgatccatt agatcctcat      960

atcaagcttc ctcatagaac gcccaattac aataagcatg ttttgctgtt atcaccgggt     1020

gataggtttg ctcaaccatg gaaggtagca cggtgtttaa accccagcgc ctggcggg       1078


<210>  22
<211>  1069
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Donor DNA for integration at HO locus

<400>  22
gacggcacgg ccacgcgttt aaaccgcccg caagtcctgt ttctatgcct ttctcttagt       60

aattcacgaa ataaacctat ggtttacgaa atgatccacg aaaatcatgt tattatttac      120

atcaacatat cgcgaaaatt catgtcatgt ccacattaac atcattgcag agcaacaatt      180

cattttcata gagaaatttg ctactatcac ccactagtac taccattggt acctactact      240

ttgaattgta ctaccgctgg gcgttattag gtgtgaaacc acgaaaagtt caccataact      300

tcgaataaag tcgcggaaaa aagtaaacag ctattgctac tcaaatgagg tttgcagaag      360

cttgttgaag catgatgaag cgttctaaac gcactattca tcattaaata tttaaagctc      420

ataaaattgt attcaattcc tattctaaat ggcttttatt tctattacaa ctattagctc      480

taaatccata tcctcataag cagcaatcaa ttctcgctcg tccaacgccg gcggacctaa      540

tgtgtatatt agtttaaaaa gttgtatgta ataaaagtaa aatttaatat tttggatgaa      600

aaaaaccatt tttagacttt ttcttaacta gaatgctgga gtagaaatac gccatctcaa      660

gatacaaaaa gcgttaccgg cactgatttg tttcaaccag tatatagatt attattgggt      720

cttgatcaac tttcctcaga catatcagta acagttatca agctaaatat ttacgcgaaa      780

gaaaaacaaa tattttaatt gtgatacttg tgaattttat tttattaagg atacaaagtt      840

aagagaaaac aaaatttata tacaatataa gtaatattca tatatatgtg atgaatgcag      900

tcttaacgag aagacatggc cttggtgaca actctcttca aaccaacttc agcctttctc      960

aattcatcag cagatgggtc ttcgatttgc aaagcagcca aagcatcgga caaagcagct     1020

tcaatcttgg acttggaacc tcggtgttta aaccccagcg cctggcggg                 1069


<210>  23
<211>  1053
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Donor DNA for integration at ADH5 locus

<400>  23
gacggcacgg ccacgcgttt aaaccgccaa ttttacaagt agtgtcttca tgacggattc       60

atagtctatc caagcgtttg cccaaaattt tgcagtaaat ttaatgtttt ctgtatagga      120

taatggtgcg ccattcaagt cccgcgaaaa atgactgatg tctacaggac aggggcgcaa      180

tatatgttct ctgacattgc accttttgaa tatatcatgt gtttgttctg ctatctgctt      240

gtagaagggt acgctaacag agccggcggc attgtaaaaa gttctccttt cgcggaagga      300

tgagtcaaaa agcatgtgac aatgaaataa tcaaattgtg acatctgctg acgcgggatc      360

gttccttcgt attgtctaga ttgtaatcta tataacatac tacgaatata aaagagggac      420

tacaagatat ttctagcgca aactactgct ttactgtctc acaatgtctc tgattggaag      480

atacctaaga aaattattta actacatatc tacaaaatca aagcatccgc tcgtccaacg      540

ccggcggacc ttcttttgta acgaatttga tgaatatatt tttacttttt atataagcta      600

ttttgtagat attgactttt tacgatttat ttgtaacaat gagaattact ccatttctga      660

acttcagtaa atagcgagtg attctgtact ttgcgagaac cggtggacat ttggtatttt      720

gccttacaag aacaacctat acaaacgttt caatatctaa ttctttgtaa tccattgttt      780

tacgagacat ataatgtgat atatagatga actttacgta taaaatgata tatttaaaac      840

tagcaactgc gtgcgtaaga caaactgaaa taggccattt acggaaaaga aatttaataa      900

tgtcgactgg aaactgaaac caggaggagt agaaattggt taaattgatt agctaaaatt      960

tactcgttgt ggacagagtt tgagccaagc ggaatgtttt caaggctttc tttgtttcga     1020

agggccggtg tttaaacccc agcgcctggc ggg                                  1053


<210>  24
<211>  1026
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: gRNA construct targeting Gal80 locus

<400>  24
ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag       60

agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc      120

gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa      180

gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagcctctt      240

tgaaaagata atgtatgatt atgctttcac tcatatttat acagaaactt gatgttttct      300

ttcgagtata tacaaggtga ttacatgtac gtttgaagta caactctaga ttttgtagtg      360

ccctcttggg ctagcggtaa aggtgcgcat tttttcacac cctacaatgt tctgttcaaa      420

agattttggt caaacgctgt agaagtgaaa gttggtgcgc atgtttcggc gttcgaaact      480

tctccgcagt gaaagataaa tgatcgtaag gctgctgctg aacgtgtttt agagctagaa      540

atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg      600

gtgctttttt tgttttttat gtctcagctt ttgttccctt tagtgagggt taattgcgcg      660

cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc      720

acacaacata ggagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgaggta      780

actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca      840

gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc      900

cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc      960

tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat     1020

gtgagc                                                                1026


<210>  25
<211>  1026
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: gRNA construct targeting HO locus

<400>  25
ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag       60

agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc      120

gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa      180

gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagcctctt      240

tgaaaagata atgtatgatt atgctttcac tcatatttat acagaaactt gatgttttct      300

ttcgagtata tacaaggtga ttacatgtac gtttgaagta caactctaga ttttgtagtg      360

ccctcttggg ctagcggtaa aggtgcgcat tttttcacac cctacaatgt tctgttcaaa      420

agattttggt caaacgctgt agaagtgaaa gttggtgcgc atgtttcggc gttcgaaact      480

tctccgcagt gaaagataaa tgatcgccgg cttgatcgac tcagagtttt agagctagaa      540

atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg      600

gtgctttttt tgttttttat gtctcagctt ttgttccctt tagtgagggt taattgcgcg      660

cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc      720

acacaacata ggagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgaggta      780

actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca      840

gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc      900

cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc      960

tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat     1020

gtgagc                                                                1026


<210>  26
<211>  1026
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: gRNA construct targeting ADH5 locus

<400>  26
ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag       60

agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc      120

gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa      180

gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagcctctt      240

tgaaaagata atgtatgatt atgctttcac tcatatttat acagaaactt gatgttttct      300

ttcgagtata tacaaggtga ttacatgtac gtttgaagta caactctaga ttttgtagtg      360

ccctcttggg ctagcggtaa aggtgcgcat tttttcacac cctacaatgt tctgttcaaa      420

agattttggt caaacgctgt agaagtgaaa gttggtgcgc atgtttcggc gttcgaaact      480

tctccgcagt gaaagataaa tgatcggggt cattggtatc gatgggtttt agagctagaa      540

atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg      600

gtgctttttt tgttttttat gtctcagctt ttgttccctt tagtgagggt taattgcgcg      660

cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc      720

acacaacata ggagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgaggta      780

actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca      840

gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc      900

cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc      960

tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat     1020

gtgagc                                                                1026


<210>  27
<211>  5975
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Vector backbone for 2-piece assembly

<400>  27
gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtggtgct ttttttgttt tttatgtctc agcttttgtt ccctttagtg      120

agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta      180

tccgctcaca attccacaca acataggagc cggaagcata aagtgtaaag cctggggtgc      240

ctaatgagtg aggtaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg      300

aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg      360

tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg      420

gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa      480

cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc      540

gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc      600

aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag      660

ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct      720

cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta      780

ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc      840

cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc      900

agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt      960

gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct     1020

gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc     1080

tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca     1140

agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta     1200

agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa     1260

atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg     1320

cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg     1380

actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc     1440

aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc     1500

cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa     1560

ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc     1620

cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg     1680

ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc     1740

cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat     1800

ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg     1860

tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc     1920

ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg     1980

aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat     2040

gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg     2100

gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg     2160

ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct     2220

catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac     2280

atttccccga aaagtgccac ctgaacgaag catctgtgct tcattttgta gaacaaaaat     2340

gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga     2400

aatgcaacgc gaaagcgcta ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca     2460

aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga     2520

acagaaatgc aacgcgagag cgctatttta ccaacaaaga atctatactt cttttttgtt     2580

ctacaaaaat gcatcccgag agcgctattt ttctaacaaa gcatcttaga ttactttttt     2640

tctcctttgt gcgctctata atgcagtctc ttgataactt tttgcactgt aggtccgtta     2700

aggttagaag aaggctactt tggtgtctat tttctcttcc ataaaaaaag cctgactcca     2760

cttcccgcgt ttactgatta ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat     2820

ccccgattat attctatacc gatgtggatt gcgcatactt tgtgaacaga aagtgatagc     2880

gttgatgatt cttcattggt cagaaaatta tgaacggttt cttctatttt gtctctatat     2940

actacgtata ggaaatgttt acattttcgt attgttttcg attcactcta tgaatagttc     3000

ttactacaat ttttttgtct aaagagtaat actagagata aacataaaaa atgtagaggt     3060

cgagtttaga tgcaagttca aggagcgaaa ggtggatggg taggttatat agggatatag     3120

cacagagata tatagcaaag agatactttt gagcaatgtt tgtggaagcg gtattcgcaa     3180

tattttagta gctcgttaca gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga     3240

gcgcttttgg ttttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc     3300

ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg     3360

cgcacataca gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg tatatatata     3420

tacatgagaa gaacggcata gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat     3480

ttatgtagga tgaaaggtag tctagtacct cctgtgatat tatcccattc catgcggggt     3540

atcgtatgct tccttcagca ctacccttta gctgttctat atgctgccac tcctcaattg     3600

gattagtctc atccttcaat gctatcattt cctttgatat tggatcatat taagaaacca     3660

ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc     3720

gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt     3780

gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg     3840

ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaaatac     3900

acatcatcgt cctacaagtt catcaaagtg ttggacagac aactatacca gcatggatct     3960

cttgtatcgg ttcttttctc ccgctctctc gcaataacaa tgaacactgg gtcaatcata     4020

gcctacacag gtgaacagag tagcgtttat acagggttta tacggtgatt cctacggcaa     4080

aaatttttca tttctaaaaa aaaaaagaaa aatttttctt tccaacgcta gaaggaaaag     4140

aaaaatctaa ttaaattgat ttggtgattt tctgagagtt ccctttttca tatatcgaat     4200

tttgaatata aaaggagatc gaaaaaattt ttctattcaa tctgttttct ggttttattt     4260

gatagttttt ttgtgtatta ttattatgga ttagtactgg tttatatggg tttttctgta     4320

taacttcttt ttattttagt ttgtttaatc ttattttgag ttacattata gttccctaac     4380

tgcaagagaa gtaacattaa aaatgaccac tcttgacgac acggcttacc ggtaccgcac     4440

cagtgtcccg ggggacgccg aggccatcga ggcactggat gggtccttca ccaccgacac     4500

cgtcttccgc gtcaccgcca ccggggacgg cttcaccctg cgggaggtgc cggtggaccc     4560

gcccctgacc aaggtgttcc ccgacgacga atcggacgac gaatcggacg ccggggagga     4620

cggcgacccg gactcccgga cgttcgtcgc gtacggggac gacggcgacc tggcgggctt     4680

cgtggtcgtc tcgtactccg gctggaaccg ccggctgacc gtcgaggaca tcgaggtcgc     4740

cccggagcac cgggggcacg gggtcgggcg cgcgttgatg gggctcgcga cggagttcgc     4800

ccgcgagcgg ggcgccgggc acctctggct ggaggtcacc aacgtcaacg caccggcgat     4860

ccacgcgtac cggcggatgg ggttcaccct ctgcggcctg gacaccgccc tgtacgacgg     4920

caccgcctcg gacggcgagc aggcgctcta catgagcatg ccctgcccct gagtttaact     4980

tgatactact agattttttc tcttcattta taaaattttt ggttataatt gaagctttag     5040

aagtatgaaa aaatcctttt ttttcattct ttgcaaccaa aataagaagc ttcttttatt     5100

cattgaaatg atgaatataa acctaacaaa agaaaaagac tcgaatatca aacattaaaa     5160

aaaaataaaa gaggttatct gttttcccat ttagttggag tttgcatttt ctaatagata     5220

gaactctcaa ttaatgtgga tttagtttct ctgttcgttt ttttttgttt tgttctcact     5280

gtatttacat ttctatttag tatttagtta ttcatataat cttaacttgc ggtgtgaaat     5340

accgcacaga tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt taatattttg     5400

ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc     5460

ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt     5520

tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc     5580

tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg     5640

tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga     5700

aagcctcttt gaaaagataa tgtatgatta tgctttcact catatttata cagaaacttg     5760

atgttttctt tcgagtatat acaaggtgat tacatgtacg tttgaagtac aactctagat     5820

tttgtagtgc cctcttgggc tagcggtaaa ggtgcgcatt ttttcacacc ctacaatgtt     5880

ctgttcaaaa gattttggtc aaacgctgta gaagtgaaag ttggtgcgca tgtttcggcg     5940

ttcgaaactt ctccgcagtg aaagataaat gatcg                                5975


<210>  28
<211>  1594
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Vector backbone fragment 1 for 3-piece assembly

<400>  28
gcaagagaag taacattaaa aatgaccact cttgacgaca cggcttaccg gtaccgcacc       60

agtgtcccgg gggacgccga ggccatcgag gcactggatg ggtccttcac caccgacacc      120

gtcttccgcg tcaccgccac cggggacggc ttcaccctgc gggaggtgcc ggtggacccg      180

cccctgacca aggtgttccc cgacgacgaa tcggacgacg aatcggacgc cggggaggac      240

ggcgacccgg actcccggac gttcgtcgcg tacggggacg acggcgacct ggcgggcttc      300

gtggtcgtct cgtactccgg ctggaaccgc cggctgaccg tcgaggacat cgaggtcgcc      360

ccggagcacc gggggcacgg ggtcgggcgc gcgttgatgg ggctcgcgac ggagttcgcc      420

cgcgagcggg gcgccgggca cctctggctg gaggtcacca acgtcaacgc accggcgatc      480

cacgcgtacc ggcggatggg gttcaccctc tgcggcctgg acaccgccct gtacgacggc      540

accgcctcgg acggcgagca ggcgctctac atgagcatgc cctgcccctg agtttaactt      600

gatactacta gattttttct cttcatttat aaaatttttg gttataattg aagctttaga      660

agtatgaaaa aatccttttt tttcattctt tgcaaccaaa ataagaagct tcttttattc      720

attgaaatga tgaatataaa cctaacaaaa gaaaaagact cgaatatcaa acattaaaaa      780

aaaataaaag aggttatctg ttttcccatt tagttggagt ttgcattttc taatagatag      840

aactctcaat taatgtggat ttagtttctc tgttcgtttt tttttgtttt gttctcactg      900

tatttacatt tctatttagt atttagttat tcatataatc ttaacttgcg gtgtgaaata      960

ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt     1020

taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg     1080

gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt     1140

ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct     1200

atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt     1260

gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa     1320

agcctctttg aaaagataat gtatgattat gctttcactc atatttatac agaaacttga     1380

tgttttcttt cgagtatata caaggtgatt acatgtacgt ttgaagtaca actctagatt     1440

ttgtagtgcc ctcttgggct agcggtaaag gtgcgcattt tttcacaccc tacaatgttc     1500

tgttcaaaag attttggtca aacgctgtag aagtgaaagt tggtgcgcat gtttcggcgt     1560

tcgaaacttc tccgcagtga aagataaatg atcg                                 1594


<210>  29
<211>  4428
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Vector backbone fragment 2 for 3-piece assembly

<400>  29
gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtggtgct ttttttgttt tttatgtctc agcttttgtt ccctttagtg      120

agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta      180

tccgctcaca attccacaca acataggagc cggaagcata aagtgtaaag cctggggtgc      240

ctaatgagtg aggtaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg      300

aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg      360

tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg      420

gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa      480

cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc      540

gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc      600

aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag      660

ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct      720

cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta      780

ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc      840

cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc      900

agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt      960

gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct     1020

gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc     1080

tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca     1140

agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta     1200

agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa     1260

atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg     1320

cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg     1380

actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc     1440

aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc     1500

cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa     1560

ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc     1620

cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg     1680

ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc     1740

cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat     1800

ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg     1860

tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc     1920

ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg     1980

aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat     2040

gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg     2100

gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg     2160

ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct     2220

catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac     2280

atttccccga aaagtgccac ctgaacgaag catctgtgct tcattttgta gaacaaaaat     2340

gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga     2400

aatgcaacgc gaaagcgcta ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca     2460

aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga     2520

acagaaatgc aacgcgagag cgctatttta ccaacaaaga atctatactt cttttttgtt     2580

ctacaaaaat gcatcccgag agcgctattt ttctaacaaa gcatcttaga ttactttttt     2640

tctcctttgt gcgctctata atgcagtctc ttgataactt tttgcactgt aggtccgtta     2700

aggttagaag aaggctactt tggtgtctat tttctcttcc ataaaaaaag cctgactcca     2760

cttcccgcgt ttactgatta ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat     2820

ccccgattat attctatacc gatgtggatt gcgcatactt tgtgaacaga aagtgatagc     2880

gttgatgatt cttcattggt cagaaaatta tgaacggttt cttctatttt gtctctatat     2940

actacgtata ggaaatgttt acattttcgt attgttttcg attcactcta tgaatagttc     3000

ttactacaat ttttttgtct aaagagtaat actagagata aacataaaaa atgtagaggt     3060

cgagtttaga tgcaagttca aggagcgaaa ggtggatggg taggttatat agggatatag     3120

cacagagata tatagcaaag agatactttt gagcaatgtt tgtggaagcg gtattcgcaa     3180

tattttagta gctcgttaca gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga     3240

gcgcttttgg ttttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc     3300

ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg     3360

cgcacataca gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg tatatatata     3420

tacatgagaa gaacggcata gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat     3480

ttatgtagga tgaaaggtag tctagtacct cctgtgatat tatcccattc catgcggggt     3540

atcgtatgct tccttcagca ctacccttta gctgttctat atgctgccac tcctcaattg     3600

gattagtctc atccttcaat gctatcattt cctttgatat tggatcatat taagaaacca     3660

ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc     3720

gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt     3780

gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg     3840

ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaaatac     3900

acatcatcgt cctacaagtt catcaaagtg ttggacagac aactatacca gcatggatct     3960

cttgtatcgg ttcttttctc ccgctctctc gcaataacaa tgaacactgg gtcaatcata     4020

gcctacacag gtgaacagag tagcgtttat acagggttta tacggtgatt cctacggcaa     4080

aaatttttca tttctaaaaa aaaaaagaaa aatttttctt tccaacgcta gaaggaaaag     4140

aaaaatctaa ttaaattgat ttggtgattt tctgagagtt ccctttttca tatatcgaat     4200

tttgaatata aaaggagatc gaaaaaattt ttctattcaa tctgttttct ggttttattt     4260

gatagttttt ttgtgtatta ttattatgga ttagtactgg tttatatggg tttttctgta     4320

taacttcttt ttattttagt ttgtttaatc ttattttgag ttacattata gttccctaac     4380

tgcaagagaa gtaacattaa aaatgaccac tcttgacgac acggctta                  4428


<210>  30
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Gal80 genomic target sequence

<400>  30
taaggctgct gctgaacgt                                                    19


<210>  31
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: HO genomic target sequence

<400>  31
ccggcttgat cgactcaga                                                    19


<210>  32
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: ADH5 genomic target sequence

<400>  32
gggtcattgg tatcgatgg                                                    19


<210>  33
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Gal80 upstream primer

<400>  33
caaacggccg cctctgccat ggcaaagaat gctttcca                               38


<210>  34
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: HO upstream primer

<400>  34
acgtgtgtgt ctcatggaaa ttgatgcagt tgaagaca                               38


<210>  35
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: ADH5 upstream primer

<400>  35
ggcgttatat ccaaacattt cagacagaag att                                    33


<210>  36
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Primer for deletion linker

<400>  36
aggtccgccg gcgttggacg agcg                                              24


<210>  37
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: TRS31 upstream primer

<400>  37
gtgcatttgg ctcgagttgc tg                                                22


<210>  38
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: TRS31 downstream primer

<400>  38
gggaagttat ctactatcat atattcattg tcacg                                  35


<210>  39
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: TRS31 heterology block primer

<400>  39
gaaaagtaga gattcagaat agatccttga c                                      31


<210>  40
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CUE5 upstream primer

<400>  40
ggaaggtatc aaggattctt ctctcc                                            26


<210>  41
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CUE5 downstream primer

<400>  41
gaggtggcac atcttcatca tcttc                                             25


<210>  42
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CUE5 heterology block primer

<400>  42
ccaataactc atcctgctcc aattgt                                            26


<210>  43
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: ECM38 upstream primer

<400>  43
cagacgctgc agtaacacaa gc                                                22


<210>  44
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: ECM38 downstream primer

<400>  44
ctgaagtggg cagttccatg c                                                 21


<210>  45
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: ECM38 heterology block primer

<400>  45
cagtgatctg gatcgtagaa gggc                                              24


<210>  46
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: PGD-1 upstream primer

<400>  46
ccaagagcat gccacggttg                                                   20


<210>  47
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: PGD-1  downstream primer

<400>  47
gagttcccat agtactaccg c                                                 21


<210>  48
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: PGD-1  heterology block primer

<400>  48
gcagacctta tctcttgtct cg                                                22


<210>  49
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: SMC6 upstream primer

<400>  49
gagctacttt cactgactgc gc                                                22


<210>  50
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: SMC6 downstream primer

<400>  50
gcgcttcaat agtagtacca tcagatg                                           27


<210>  51
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: SMC6 heterology block primer

<400>  51
gccgttctct gatctcaaag agaat                                             25


<210>  52
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: NTO1  upstream primer

<400>  52
ctcagtatga catggatgaa caggatg                                           27


<210>  53
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: NTO1 downstream primer

<400>  53
ggtacctcct gtaagctccc ttttc                                             25


<210>  54
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: NTO1 heterology block primer

<400>  54
gactgagacg ttctggactc cttc                                              24


<210>  55
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: DGA1 upstream primer

<400>  55
cttaaccaag cacgacagtg gtc                                               23


<210>  56
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: DGA1 downstream primer

<400>  56
gattccctag cgccaccaac                                                   20


<210>  57
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: DGA1 heterology block primer

<400>  57
cctctccggt ggctggtgat ctg                                               23


<210>  58
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: ADH2 upstream primer

<400>  58
cgagactgat ctcctctgcc ggaac                                             25


<210>  59
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: ADH2 downstream primer

<400>  59
gaatacttca ccaccgagcg ag                                                22


<210>  60
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: ADH2 heterology block primer

<400>  60
gcatgtaagt ctgtatgaca tactcctg                                          28


<210>  61
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: SIN4 upstream primer

<400>  61
caaacgtcct aaatgaccca tcgttg                                            26


<210>  62
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: SIN4 downstream primer

<400>  62
caacttcggg ttttgttgtt ggttag                                            26


<210>  63
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: SIN4 heterology block primer

<400>  63
caatggcaat ttaccgtagt tgaaaccg                                          28


<210>  64
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CYS4 upstream primer

<400>  64
ctccagaatc acatattggt gttgc                                             25


<210>  65
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CYS4 downstream primer

<400>  65
ccatcttagt aacgatatgg attggtttc                                         29


<210>  66
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CYS4 heterology block primer

<400>  66
ctgatggagt caggaaagat ggc                                               23


<210>  67
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: TRS31 donor DNA

<400>  67
aagcaagcga taacaagcga agcttcaaca acatacattc cgtcaaggat ctattctgaa       60

tctctacttt tcaaaaggca agaagcgtct ttatcagcaa                            100


<210>  68
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: TRS31 locus target sequence

<400>  68
gactttgagt atattctaga gg                                                22


<210>  69
<211>  1080
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: TRS31 gRNA construct

<400>  69
gacggcacgg ccacgcgttt aaaccgcctt ataaatcaaa agaatagacc gagatagggt       60

tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca      120

aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa      180

gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat      240

ttagagcttg acggggaaag cctctttgaa aagataatgt atgattatgc tttcactcat      300

atttatacag aaacttgatg ttttctttcg agtatataca aggtgattac atgtacgttt      360

gaagtacaac tctagatttt gtagtgccct cttgggctag cggtaaaggt gcgcattttt      420

tcacacccta caatgttctg ttcaaaagat tttggtcaaa cgctgtagaa gtgaaagttg      480

gtgcgcatgt ttcggcgttc gaaacttctc cgcagtgaaa gataaatgat cggactttga      540

gtatattcta ggttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa      600

cttgaaaaag tggcaccgag tcggtggtgc tttttttgtt ttttatgtct cagcttttgt      660

tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg      720

tgaaattgtt atccgctcac aattccacac aacataggag ccggaagcat aaagtgtaaa      780

gcctggggtg cctaatgagt gaggtaactc acattaattg cgttgcgctc actgcccgct      840

ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga      900

ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc      960

gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa     1020

tcaggggata acgcaggaaa gaacatgtga gccggtgttt aaaccccagc gcctggcggg     1080


<210>  70
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CUE5 donor DNA

<400>  70
gtaagaaaga acccagaggc gccggcaaga agacgacaaa cacaattgga gcaggatgag       60

ttattggcaa ggcaattaga tgagcaattc aacagctcac                            100


<210>  71
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CUE5 locus target sequence

<400>  71
aagtttgtcg tcttcttgcc gg                                                22


<210>  72
<211>  1080
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CUE5 gRNA construct

<400>  72
gacggcacgg ccacgcgttt aaaccgcctt ataaatcaaa agaatagacc gagatagggt       60

tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca      120

aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa      180

gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat      240

ttagagcttg acggggaaag cctctttgaa aagataatgt atgattatgc tttcactcat      300

atttatacag aaacttgatg ttttctttcg agtatataca aggtgattac atgtacgttt      360

gaagtacaac tctagatttt gtagtgccct cttgggctag cggtaaaggt gcgcattttt      420

tcacacccta caatgttctg ttcaaaagat tttggtcaaa cgctgtagaa gtgaaagttg      480

gtgcgcatgt ttcggcgttc gaaacttctc cgcagtgaaa gataaatgat cgaagtttgt      540

cgtcttcttg cgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa      600

cttgaaaaag tggcaccgag tcggtggtgc tttttttgtt ttttatgtct cagcttttgt      660

tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg      720

tgaaattgtt atccgctcac aattccacac aacataggag ccggaagcat aaagtgtaaa      780

gcctggggtg cctaatgagt gaggtaactc acattaattg cgttgcgctc actgcccgct      840

ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga      900

ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc      960

gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa     1020

tcaggggata acgcaggaaa gaacatgtga gccggtgttt aaaccccagc gcctggcggg     1080


<210>  73
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: ECM38 donor DNA

<400>  73
tctaacatgc taatggaact ggctaaaaat ggttccgtgg cgcccttcta cgatccagat       60

cactggattg caaaatccat gattgatact gtcgcaaaat                            100


<210>  74
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: ECM38 locus target sequence

<400>  74
tgatcgaggt cataaaatgg gg                                                22


<210>  75
<211>  1080
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: ECM38 gRNA construct

<400>  75
gacggcacgg ccacgcgttt aaaccgcctt ataaatcaaa agaatagacc gagatagggt       60

tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca      120

aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa      180

gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat      240

ttagagcttg acggggaaag cctctttgaa aagataatgt atgattatgc tttcactcat      300

atttatacag aaacttgatg ttttctttcg agtatataca aggtgattac atgtacgttt      360

gaagtacaac tctagatttt gtagtgccct cttgggctag cggtaaaggt gcgcattttt      420

tcacacccta caatgttctg ttcaaaagat tttggtcaaa cgctgtagaa gtgaaagttg      480

gtgcgcatgt ttcggcgttc gaaacttctc cgcagtgaaa gataaatgat cgtgatcgag      540

gtcataaaat ggttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa      600

cttgaaaaag tggcaccgag tcggtggtgc tttttttgtt ttttatgtct cagcttttgt      660

tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg      720

tgaaattgtt atccgctcac aattccacac aacataggag ccggaagcat aaagtgtaaa      780

gcctggggtg cctaatgagt gaggtaactc acattaattg cgttgcgctc actgcccgct      840

ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga      900

ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc      960

gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa     1020

tcaggggata acgcaggaaa gaacatgtga gccggtgttt aaaccccagc gcctggcggg     1080


<210>  76
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: PGD-1 donor DNA

<400>  76
ggcgtcaagc tagatgattt acaagtgata ttggctaaaa atgaaaacga gacaagagat       60

aaggtctgca agcagatcaa cgaagcacgc gatgaaattc                            100


<210>  77
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: PGD-1 locus target sequence

<400>  77
aagaacgaga atgaaaccag gg                                                22


<210>  78
<211>  1080
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: PGD-1 gRNA construct

<400>  78
gacggcacgg ccacgcgttt aaaccgcctt ataaatcaaa agaatagacc gagatagggt       60

tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca      120

aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa      180

gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat      240

ttagagcttg acggggaaag cctctttgaa aagataatgt atgattatgc tttcactcat      300

atttatacag aaacttgatg ttttctttcg agtatataca aggtgattac atgtacgttt      360

gaagtacaac tctagatttt gtagtgccct cttgggctag cggtaaaggt gcgcattttt      420

tcacacccta caatgttctg ttcaaaagat tttggtcaaa cgctgtagaa gtgaaagttg      480

gtgcgcatgt ttcggcgttc gaaacttctc cgcagtgaaa gataaatgat cgaagaacga      540

gaatgaaacc agttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa      600

cttgaaaaag tggcaccgag tcggtggtgc tttttttgtt ttttatgtct cagcttttgt      660

tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg      720

tgaaattgtt atccgctcac aattccacac aacataggag ccggaagcat aaagtgtaaa      780

gcctggggtg cctaatgagt gaggtaactc acattaattg cgttgcgctc actgcccgct      840

ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga      900

ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc      960

gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa     1020

tcaggggata acgcaggaaa gaacatgtga gccggtgttt aaaccccagc gcctggcggg     1080


<210>  79
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: SMC6 donor DNA

<400>  79
cgaaattata gtagaaagga taattaagag agatgggccc gcttcattct ctttgagatc       60

agagaacggc aaggaaatta gcaataagaa aaaggatatt                            100


<210>  80
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: SMC6 locus target sequence

<400>  80
gcttctttta gattaaggtc gg                                                22


<210>  81
<211>  1080
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: SMC6 gRNA construct

<400>  81
gacggcacgg ccacgcgttt aaaccgcctt ataaatcaaa agaatagacc gagatagggt       60

tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca      120

aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa      180

gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat      240

ttagagcttg acggggaaag cctctttgaa aagataatgt atgattatgc tttcactcat      300

atttatacag aaacttgatg ttttctttcg agtatataca aggtgattac atgtacgttt      360

gaagtacaac tctagatttt gtagtgccct cttgggctag cggtaaaggt gcgcattttt      420

tcacacccta caatgttctg ttcaaaagat tttggtcaaa cgctgtagaa gtgaaagttg      480

gtgcgcatgt ttcggcgttc gaaacttctc cgcagtgaaa gataaatgat cggcttcttt      540

tagattaagg tgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa      600

cttgaaaaag tggcaccgag tcggtggtgc tttttttgtt ttttatgtct cagcttttgt      660

tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg      720

tgaaattgtt atccgctcac aattccacac aacataggag ccggaagcat aaagtgtaaa      780

gcctggggtg cctaatgagt gaggtaactc acattaattg cgttgcgctc actgcccgct      840

ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga      900

ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc      960

gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa     1020

tcaggggata acgcaggaaa gaacatgtga gccggtgttt aaaccccagc gcctggcggg     1080


<210>  82
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: NTO1 donor DNA

<400>  82
atacttttca aacttacatt atatggaacc tatagaagga gtccagaacg tctcagtctc       60

taggtggaaa cttaactgct acatctgtaa aaagaagat                              99


<210>  83
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: NTO1 locus target sequence

<400>  83
aaatgttagc gtatcacgat gg                                                22


<210>  84
<211>  1080
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: NTO1 gRNA construct

<400>  84
gacggcacgg ccacgcgttt aaaccgcctt ataaatcaaa agaatagacc gagatagggt       60

tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca      120

aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa      180

gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat      240

ttagagcttg acggggaaag cctctttgaa aagataatgt atgattatgc tttcactcat      300

atttatacag aaacttgatg ttttctttcg agtatataca aggtgattac atgtacgttt      360

gaagtacaac tctagatttt gtagtgccct cttgggctag cggtaaaggt gcgcattttt      420

tcacacccta caatgttctg ttcaaaagat tttggtcaaa cgctgtagaa gtgaaagttg      480

gtgcgcatgt ttcggcgttc gaaacttctc cgcagtgaaa gataaatgat cgaaatgtta      540

gcgtatcacg agttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa      600

cttgaaaaag tggcaccgag tcggtggtgc tttttttgtt ttttatgtct cagcttttgt      660

tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg      720

tgaaattgtt atccgctcac aattccacac aacataggag ccggaagcat aaagtgtaaa      780

gcctggggtg cctaatgagt gaggtaactc acattaattg cgttgcgctc actgcccgct      840

ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga      900

ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc      960

gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa     1020

tcaggggata acgcaggaaa gaacatgtga gccggtgttt aaaccccagc gcctggcggg     1080


<210>  85
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: DGA1 donor DNA

<400>  85
gggttcttgc tattccatat atgatttatt ttttttttga cagatcacca gccaccggag       60

aggttgtcaa tcgatactct cttcgatttc gttcattgc                              99


<210>  86
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: DGA1 locus target sequence

<400>  86
tcgataggtc tcctgcaact gg                                                22


<210>  87
<211>  1080
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: DGA1 gRNA construct

<400>  87
gacggcacgg ccacgcgttt aaaccgcctt ataaatcaaa agaatagacc gagatagggt       60

tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca      120

aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa      180

gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat      240

ttagagcttg acggggaaag cctctttgaa aagataatgt atgattatgc tttcactcat      300

atttatacag aaacttgatg ttttctttcg agtatataca aggtgattac atgtacgttt      360

gaagtacaac tctagatttt gtagtgccct cttgggctag cggtaaaggt gcgcattttt      420

tcacacccta caatgttctg ttcaaaagat tttggtcaaa cgctgtagaa gtgaaagttg      480

gtgcgcatgt ttcggcgttc gaaacttctc cgcagtgaaa gataaatgat cgtcgatagg      540

tctcctgcaa cgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa      600

cttgaaaaag tggcaccgag tcggtggtgc tttttttgtt ttttatgtct cagcttttgt      660

tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg      720

tgaaattgtt atccgctcac aattccacac aacataggag ccggaagcat aaagtgtaaa      780

gcctggggtg cctaatgagt gaggtaactc acattaattg cgttgcgctc actgcccgct      840

ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga      900

ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc      960

gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa     1020

tcaggggata acgcaggaaa gaacatgtga gccggtgttt aaaccccagc gcctggcggg     1080


<210>  88
<211>  929
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: ADH2 donor DNA

<400>  88
ccgggcatct ccaacttata agttggagaa ataagagaat ttcagattga gagaatgaaa       60

aaaaaaaaaa aaaaaaaggc agaggagagc ataaaaatgg ggttcacttt ttggtaaagc      120

tatagcatgc ctatcacata taaatagagt gccagtagcg acttttttca cactcgaaat      180

actcttacta ctgctctctt gttgttttta tcacttcttg tttcttcttg gtaaatagaa      240

tatcaagcta caaaaagcat acaatcaact atcaactatt aactatatcg taatacacaa      300

tgtctattcc agaaactcaa aaagccatta tcttctacga atccaacggc aagttggagc      360

ataaggatat cccagttcca aagccaaagc ccaacgaatt gttaatcaac gtcaaatatt      420

caggagtatg tcatacagac ttacatgcat ggcatggtga ctggccattg ccaactaagt      480

taccattagt tggtgggcac gaaggtgctg gggtcgttgt tgccatgggt gaaaacgtta      540

agggctggaa gatcggtgac tacgccggta ttaaatggtt gaacagttca tgtatggcct      600

gcgaatactg tgaattgggt aacgaatcca actgtcctca cgctgacttg tctggttaca      660

cccatgacgg ttctttccaa caatacgcta ctgctgacgc ggttcaagcc gctcacattc      720

ctcaaggtac tgacttggct gaagtcgccc ccatcttgtg tgctggtatc accgtataca      780

aggctttgaa gtctgccaac ttgagagcag gccactgggt ggccatttct ggtgctgctg      840

gtggtctagg ttctttggct gttcaatatg ctaaggcgat gggttacaga gtcttaggta      900

ttgatggtgg tccaggaaag gaagaattg                                        929


<210>  89
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: ADH2 locus target sequence

<400>  89
taatcaacgt caaatacttt gg                                                22


<210>  90
<211>  1080
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: ADH2 gRNA construct

<400>  90
gacggcacgg ccacgcgttt aaaccgcctt ataaatcaaa agaatagacc gagatagggt       60

tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca      120

aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa      180

gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat      240

ttagagcttg acggggaaag cctctttgaa aagataatgt atgattatgc tttcactcat      300

atttatacag aaacttgatg ttttctttcg agtatataca aggtgattac atgtacgttt      360

gaagtacaac tctagatttt gtagtgccct cttgggctag cggtaaaggt gcgcattttt      420

tcacacccta caatgttctg ttcaaaagat tttggtcaaa cgctgtagaa gtgaaagttg      480

gtgcgcatgt ttcggcgttc gaaacttctc cgcagtgaaa gataaatgat cgtaatcaac      540

gtcaaatact tgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa      600

cttgaaaaag tggcaccgag tcggtggtgc tttttttgtt ttttatgtct cagcttttgt      660

tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg      720

tgaaattgtt atccgctcac aattccacac aacataggag ccggaagcat aaagtgtaaa      780

gcctggggtg cctaatgagt gaggtaactc acattaattg cgttgcgctc actgcccgct      840

ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga      900

ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc      960

gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa     1020

tcaggggata acgcaggaaa gaacatgtga gccggtgttt aaaccccagc gcctggcggg     1080


<210>  91
<211>  979
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: SIN4 donor DNA

<400>  91
gcactacttt agatccaacg gatgatgaag ggcatgtgtt gaagttagaa aacctgcatg       60

ttgtgtctaa atcatcaatt gagaaagatc cctccccaga aattttagtt ttgtataatg      120

tttgcgatac atcaaaatca ttggtgaaaa gatatagact agctccaaca caactttcag      180

ctgagtattt ggtgatattg aaaccagatt taaatatcga tcgaaataat tctacgaatc      240

aaatattcca atcacgccgt tacaaccttc gtcgccacag tgatatcgtt ctcgacaaaa      300

aagtcacctt gataacttct gagatgttcg atggatttgt ttcattttac tttgaagatg      360

gtaccatcga atcctacaat caaaatgatt ggaagttgga gactgaacga cttataggtc      420

agagccaatt aggaaaattc aaaaatatta ttgcatcccc actaagcgcc ggtttcaact      480

acggtaaatt gccattgcca ccatcggttg agtggatgaa ggtttcacca tctatgtgtg      540

gggttatcgt taaacagtat aataaaaaat ggccacagtt ttatgctgct gttcaaaaaa      600

actacgcaga tcctgagaag gattctataa atgctacagc tttggcattt ggatacgtga      660

aaagtttaca taagcaaata tctgccgaag acttgaccat cgctgcgaag acgcacatac      720

tcagaatttc attcttggat agaaaaaggg ccaaagaatt tattacgact ttgttgaaaa      780

gcctatactc gtttttcaac atttctcctg atgcgcctaa agagattatg gataaaataa      840

taacaagtag gccacttcaa aaaattatgc tattgcagct ggaacttggc agttgttttt      900

cgcaagaaaa tattgaagaa atggcgagag ttatactcta cttgaaaaat gttttgtttg      960

cgttcaatgg cgtggcgag                                                   979


<210>  92
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: SIN4  locus target sequence

<400>  92
actcaaccga tggaggtaaa gg                                                22


<210>  93
<211>  1080
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: SIN4 gRNA construct

<400>  93
gacggcacgg ccacgcgttt aaaccgcctt ataaatcaaa agaatagacc gagatagggt       60

tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca      120

aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa      180

gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat      240

ttagagcttg acggggaaag cctctttgaa aagataatgt atgattatgc tttcactcat      300

atttatacag aaacttgatg ttttctttcg agtatataca aggtgattac atgtacgttt      360

gaagtacaac tctagatttt gtagtgccct cttgggctag cggtaaaggt gcgcattttt      420

tcacacccta caatgttctg ttcaaaagat tttggtcaaa cgctgtagaa gtgaaagttg      480

gtgcgcatgt ttcggcgttc gaaacttctc cgcagtgaaa gataaatgat cgactcaacc      540

gatggaggta agttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa      600

cttgaaaaag tggcaccgag tcggtggtgc tttttttgtt ttttatgtct cagcttttgt      660

tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg      720

tgaaattgtt atccgctcac aattccacac aacataggag ccggaagcat aaagtgtaaa      780

gcctggggtg cctaatgagt gaggtaactc acattaattg cgttgcgctc actgcccgct      840

ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga      900

ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc      960

gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa     1020

tcaggggata acgcaggaaa gaacatgtga gccggtgttt aaaccccagc gcctggcggg     1080


<210>  94
<211>  1038
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CYS4 donor DNA

<400>  94
gttggaaaaa gagattcctg gtgctgttat acttgaccaa tataacaata tgatgaaccc       60

agaagctcat tactttggta ctggtcgcga aatccaaaga cagctagaag acttgaattt      120

atttgataat ctacgcgctg ttgttgctgg tgctggtact ggtgggacta ttagcggtat      180

ttccaagtac ttgaaagaac agaatgataa gatccaaatc gttggtgctg acccattcgg      240

ttcaatttta gcccaacctg aaaacttgaa taagactgat atcactgact acaaagttga      300

gggtattggt tatgattttg ttcctcaggt tttggacaga aaattaattg atgtttggta      360

taagacagac gacaagcctt ctttcaaata cgccagacaa ttgatttcta acgaaggtgt      420

cttggtgggt ggttcttccg gttctgcctt cactgcggtt gtgaaatact gtgaagacca      480

ccctgaactg actgaagatg atgtcatcgt cgccatcttt cctgactcca tcaggtcgta      540

cctaaccaaa ttcgtcgatg acgaatggtt gaaaaagaac aatttgtggg atgatgacgt      600

gttggcccgt tttgactctt caaagctgga ggcttcgacg acaaaatacg ctgatgtgtt      660

tggtaacgct actgtaaagg atcttcactt gaaaccggtt gtttccgtta aggaaaccgc      720

taaggtcact gatgttatca agatattaaa agacaatggc tttgaccaat tgcctgtgtt      780

gactgaagac ggcaagttgt ctggtttagt tactctctct gagcttctaa gaaaactatc      840

aatcaataat tcaaacaacg acaacactat aaagggtaaa tacttggact tcaagaaatt      900

aaacaatttc aatgatgttt cctcttacaa cgaaaataaa tccggtaaga agaagtttat      960

taaattcgat gaaaactcaa agctatctga cttgaatcgt ttctttgaaa aaaactcatc     1020

tgccgttatc actgatgg                                                   1038


<210>  95
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CYS4 locus target sequence

<400>  95
ggtacgacct gatggaatct gg                                                22


<210>  96
<211>  1080
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CYS4  gRNA construct

<400>  96
gacggcacgg ccacgcgttt aaaccgcctt ataaatcaaa agaatagacc gagatagggt       60

tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca      120

aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa      180

gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat      240

ttagagcttg acggggaaag cctctttgaa aagataatgt atgattatgc tttcactcat      300

atttatacag aaacttgatg ttttctttcg agtatataca aggtgattac atgtacgttt      360

gaagtacaac tctagatttt gtagtgccct cttgggctag cggtaaaggt gcgcattttt      420

tcacacccta caatgttctg ttcaaaagat tttggtcaaa cgctgtagaa gtgaaagttg      480

gtgcgcatgt ttcggcgttc gaaacttctc cgcagtgaaa gataaatgat cgggtacgac      540

ctgatggaat cgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa      600

cttgaaaaag tggcaccgag tcggtggtgc tttttttgtt ttttatgtct cagcttttgt      660

tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg      720

tgaaattgtt atccgctcac aattccacac aacataggag ccggaagcat aaagtgtaaa      780

gcctggggtg cctaatgagt gaggtaactc acattaattg cgttgcgctc actgcccgct      840

ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga      900

ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc      960

gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa     1020

tcaggggata acgcaggaaa gaacatgtga gccggtgttt aaaccccagc gcctggcggg     1080


<210>  97
<211>  8045
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: pAM3472

<400>  97
gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta       60

ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag      120

ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc      180

ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga      240

cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat      300

atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc      360

cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct      420

attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca      480

cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat      540

caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg      600

cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg      660

agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg      720

agcggccgcc accatgggca agcccatccc taaccccctg ttggggctgg acagcaccgc      780

tcccaaaaag aaaaggaagg tgggcattca cggcgtgcct gcggccgaca aaaagtacag      840

catcggcctt gatatcggca ccaatagcgt gggctgggcc gttatcacag acgaatacaa      900

ggtacccagc aagaagttca aggtgctggg gaatacagac aggcactcta tcaagaaaaa      960

ccttatcggg gctctgctgt ttgactcagg cgagaccgcc gaggccacca ggttgaagag     1020

gaccgcaagg cgaaggtaca cccggaggaa gaacaggatc tgctatctgc aggagatctt     1080

cagcaacgag atggccaagg tggacgacag cttcttccac aggctggagg agagcttcct     1140

tgtcgaggag gataagaagc acgaacgaca ccccatcttc ggcaacatag tcgacgaggt     1200

cgcttatcac gagaagtacc ccaccatcta ccacctgcga aagaaattgg tggatagcac     1260

cgataaagcc gacttgcgac ttatctactt ggctctggcg cacatgatta agttcagggg     1320

ccacttcctg atcgagggcg accttaaccc cgacaacagt gacgtagaca aattgttcat     1380

ccagcttgta cagacctata accagctgtt cgaggaaaac cctattaacg ccagcggggt     1440

ggatgcgaag gccatactta gcgccaggct gagcaaaagc aggcgcttgg agaacctgat     1500

agcccagctg cccggtgaaa agaagaacgg cctcttcggt aatctgattg ccctgagcct     1560

gggcctgacc cccaacttca agagcaactt cgacctggca gaagatgcca agctgcagtt     1620

gagtaaggac acctatgacg acgacttgga caatctgctc gcccaaatcg gcgaccagta     1680

cgctgacctg ttcctcgccg ccaagaacct ttctgacgca atcctgctta gcgatatcct     1740

tagggtgaac acagagatca ccaaggcccc cctgagcgcc agcatgatca agaggtacga     1800

cgagcaccat caggacctga cccttctgaa ggccctggtg aggcagcaac tgcccgagaa     1860

gtacaaggag atctttttcg accagagcaa gaacggctac gccggctaca tcgacggcgg     1920

agccagccaa gaggagttct acaagttcat caagcccatc ctggagaaga tggatggcac     1980

cgaggagctg ctggtgaagc tgaacaggga agatttgctc cggaagcaga ggacctttga     2040

caacggtagc atcccccacc agatccacct gggcgagctg cacgcaatac tgaggcgaca     2100

ggaggatttc taccccttcc tcaaggacaa tagggagaaa atcgaaaaga ttctgacctt     2160

caggatcccc tactacgtgg gccctcttgc caggggcaac agccgattcg cttggatgac     2220

aagaaagagc gaggagacca tcaccccctg gaacttcgag gaagtggtgg acaaaggagc     2280

aagcgcgcag tctttcatcg aacggatgac caatttcgac aaaaacctgc ctaacgagaa     2340

ggtgctgccc aagcacagcc tgctttacga gtacttcacc gtgtacaacg agctcaccaa     2400

ggtgaaatat gtgaccgagg gcatgcgaaa acccgctttc ctgagcggcg agcagaagaa     2460

ggccatcgtg gacctgctgt tcaagaccaa caggaaggtg accgtgaagc agctgaagga     2520

ggactacttc aagaagatcg agtgctttga tagcgtggaa ataagcggcg tggaggacag     2580

gttcaacgcc agcctgggca cctaccacga cttgttgaag ataatcaaag acaaggattt     2640

cctggataat gaggagaacg aggatatact cgaggacatc gtgctgactt tgaccctgtt     2700

tgaggaccga gagatgattg aagaaaggct caaaacctac gcccacctgt tcgacgacaa     2760

agtgatgaaa caactgaaga gacgaagata caccggctgg ggcagactgt ccaggaagct     2820

catcaacggc attagggaca agcagagcgg caagaccatc ctggatttcc tgaagtccga     2880

cggcttcgcc aaccgaaact tcatgcagct gattcacgat gacagcttga ccttcaagga     2940

ggacatccag aaggcccagg ttagcggcca gggcgactcc ctgcacgaac atattgcaaa     3000

cctggcaggc tcccctgcga tcaagaaggg catactgcag accgttaagg ttgtggacga     3060

attggtcaag gtcatgggca ggcacaagcc cgaaaacata gttatagaga tggccagaga     3120

gaaccagacc acccaaaagg gccagaagaa cagccgggag cgcatgaaaa ggatcgagga     3180

gggtatcaag gaactcggaa gccagatcct caaagagcac cccgtggaga atacccagct     3240

ccagaacgag aagctgtacc tgtactacct gcagaacggc agggacatgt acgttgacca     3300

ggagttggac atcaacaggc tttcagacta tgacgtggat cacatagtgc cccagagctt     3360

tcttaaagac gatagcatcg acaacaaggt cctgacccgc tccgacaaaa acaggggcaa     3420

aagcgacaac gtgccaagcg aagaggtggt taaaaagatg aagaactact ggaggcaact     3480

gctcaacgcg aaattgatca cccagagaaa gttcgataac ctgaccaagg ccgagagggg     3540

cggactctcc gaacttgaca aagcgggctt cataaagagg cagctggtcg agacccgaca     3600

gatcacgaag cacgtggccc aaatcctcga cagcagaatg aataccaagt acgatgagaa     3660

tgacaaactc atcagggaag tgaaagtgat taccctgaag agcaagttgg tgtccgactt     3720

tcgcaaagat ttccagttct acaaggtgag ggagatcaac aactaccacc atgcccacga     3780

cgcatacctg aacgccgtgg tcggcaccgc cctgattaag aagtatccaa agctggagtc     3840

cgaatttgtc tacggcgact acaaagttta cgatgtgagg aagatgatcg ctaagagcga     3900

acaggagatc ggcaaggcca ccgctaagta tttcttctac agcaacatca tgaacttttt     3960

caagaccgag atcacacttg ccaacggcga aatcaggaag aggccgctta tcgagaccaa     4020

cggtgagacc ggcgagatcg tgtgggacaa gggcagggac ttcgccaccg tgaggaaagt     4080

cctgagcatg ccccaggtga atattgtgaa aaaaactgag gtgcagacag gcggctttag     4140

caaggaatcc atcctgccca agaggaacag cgacaagctg atcgcccgga agaaggactg     4200

ggaccctaag aagtatggag gcttcgacag ccccaccgta gcctacagcg tgctggtggt     4260

cgcgaaggta gagaagggga agagcaagaa actgaagagc gtgaaggagc tgctcggcat     4320

aaccatcatg gagaggtcca gctttgagaa gaaccccatt gactttttgg aagccaaggg     4380

ctacaaagag gtcaaaaagg acctgatcat caaactcccc aagtactccc tgtttgaatt     4440

ggagaacggc agaaagagga tgctggcgag cgctggggaa ctgcaaaagg gcaacgaact     4500

ggcgctgccc agcaagtacg tgaattttct gtacctggcg tcccactacg aaaagctgaa     4560

aggcagcccc gaggacaacg agcagaagca gctgttcgtg gagcagcaca agcattacct     4620

ggacgagata atcgagcaaa tcagcgagtt cagcaagagg gtgattctgg ccgacgcgaa     4680

cctggataag gtcctcagcg cctacaacaa gcaccgagac aaacccatca gggagcaggc     4740

cgagaatatc atacacctgt tcaccctgac aaatctgggc gcacctgcgg cattcaaata     4800

cttcgatacc accatcgaca ggaaaaggta cactagcact aaggaggtgc tggatgccac     4860

cttgatccac cagtccatta ccggcctgta tgagaccagg atcgacctga gccagcttgg     4920

aggcgactct agggcggacc caaaaaagaa aaggaaggtg gaattctcta gaggcagtgg     4980

agagggcaga ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg gcccaatgaa     5040

ccggggagtc ccttttaggc acttgcttct ggtgctgcaa ctggcgctcc tcccagcagc     5100

cactcaggga aagaaagtgg tgctgggcaa aaaaggggat acagtggaac tgacctgtac     5160

agcttcccag aagaagagca tacaattcca ctggaaaaac tccaaccaga taaagattct     5220

gggaaatcag ggctccttct taactaaagg tccatccaag ctgaatgatc gcgctgactc     5280

aagaagaagc ctttgggacc aaggaaactt ccccctgatc atcaagaatc ttaagataga     5340

agactcagat acttacatct gtgaagtgga ggaccagaag gaggaggtgc aattgctagt     5400

gttcggattg actgccaact ctgacaccca cctgcttcag gggcagagcc tgaccctgac     5460

cttggagagc ccccctggta gtagcccctc agtgcaatgt aggagtccaa ggggtaaaaa     5520

catacagggg gggaagaccc tctccgtgtc tcagctggag ctccaggata gtggcacctg     5580

gacatgcact gtcttgcaga accagaagaa ggtggagttc aaaatagaca tcgtggtgct     5640

agcttgataa atcgattagc ccgggtaccg tcgacctcta gctagagctt ggcgtaatca     5700

tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga     5760

gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt     5820

gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga     5880

atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc     5940

actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg     6000

gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc     6060

cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc     6120

ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga     6180

ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc     6240

ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat     6300

agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg     6360

cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc     6420

aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga     6480

gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact     6540

agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt     6600

ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag     6660

cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg     6720

tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa     6780

aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata     6840

tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg     6900

atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata     6960

cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg     7020

gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct     7080

gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt     7140

tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc     7200

tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga     7260

tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt     7320

aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc     7380

atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa     7440

tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca     7500

catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca     7560

aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct     7620

tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc     7680

gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa     7740

tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt     7800

tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc     7860

gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg     7920

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg     7980

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc     8040

ttagg                                                                 8045


<210>  98
<211>  9823
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: pAM3473

<400>  98
gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta       60

ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag      120

ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc      180

ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga      240

cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat      300

atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc      360

cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct      420

attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca      480

cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat      540

caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg      600

cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg      660

agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg      720

agcggccgcc accatgggca agcccatccc taaccccctg ttggggctgg acagcaccgc      780

tcccaaaaag aaaaggaagg tgggcattca cggcgtgcct gcggccgaca aaaagtacag      840

catcggcctt gatatcggca ccaatagcgt gggctgggcc gttatcacag acgaatacaa      900

ggtacccagc aagaagttca aggtgctggg gaatacagac aggcactcta tcaagaaaaa      960

ccttatcggg gctctgctgt ttgactcagg cgagaccgcc gaggccacca ggttgaagag     1020

gaccgcaagg cgaaggtaca cccggaggaa gaacaggatc tgctatctgc aggagatctt     1080

cagcaacgag atggccaagg tggacgacag cttcttccac aggctggagg agagcttcct     1140

tgtcgaggag gataagaagc acgaacgaca ccccatcttc ggcaacatag tcgacgaggt     1200

cgcttatcac gagaagtacc ccaccatcta ccacctgcga aagaaattgg tggatagcac     1260

cgataaagcc gacttgcgac ttatctactt ggctctggcg cacatgatta agttcagggg     1320

ccacttcctg atcgagggcg accttaaccc cgacaacagt gacgtagaca aattgttcat     1380

ccagcttgta cagacctata accagctgtt cgaggaaaac cctattaacg ccagcggggt     1440

ggatgcgaag gccatactta gcgccaggct gagcaaaagc aggcgcttgg agaacctgat     1500

agcccagctg cccggtgaaa agaagaacgg cctcttcggt aatctgattg ccctgagcct     1560

gggcctgacc cccaacttca agagcaactt cgacctggca gaagatgcca agctgcagtt     1620

gagtaaggac acctatgacg acgacttgga caatctgctc gcccaaatcg gcgaccagta     1680

cgctgacctg ttcctcgccg ccaagaacct ttctgacgca atcctgctta gcgatatcct     1740

tagggtgaac acagagatca ccaaggcccc cctgagcgcc agcatgatca agaggtacga     1800

cgagcaccat caggacctga cccttctgaa ggccctggtg aggcagcaac tgcccgagaa     1860

gtacaaggag atctttttcg accagagcaa gaacggctac gccggctaca tcgacggcgg     1920

agccagccaa gaggagttct acaagttcat caagcccatc ctggagaaga tggatggcac     1980

cgaggagctg ctggtgaagc tgaacaggga agatttgctc cggaagcaga ggacctttga     2040

caacggtagc atcccccacc agatccacct gggcgagctg cacgcaatac tgaggcgaca     2100

ggaggatttc taccccttcc tcaaggacaa tagggagaaa atcgaaaaga ttctgacctt     2160

caggatcccc tactacgtgg gccctcttgc caggggcaac agccgattcg cttggatgac     2220

aagaaagagc gaggagacca tcaccccctg gaacttcgag gaagtggtgg acaaaggagc     2280

aagcgcgcag tctttcatcg aacggatgac caatttcgac aaaaacctgc ctaacgagaa     2340

ggtgctgccc aagcacagcc tgctttacga gtacttcacc gtgtacaacg agctcaccaa     2400

ggtgaaatat gtgaccgagg gcatgcgaaa acccgctttc ctgagcggcg agcagaagaa     2460

ggccatcgtg gacctgctgt tcaagaccaa caggaaggtg accgtgaagc agctgaagga     2520

ggactacttc aagaagatcg agtgctttga tagcgtggaa ataagcggcg tggaggacag     2580

gttcaacgcc agcctgggca cctaccacga cttgttgaag ataatcaaag acaaggattt     2640

cctggataat gaggagaacg aggatatact cgaggacatc gtgctgactt tgaccctgtt     2700

tgaggaccga gagatgattg aagaaaggct caaaacctac gcccacctgt tcgacgacaa     2760

agtgatgaaa caactgaaga gacgaagata caccggctgg ggcagactgt ccaggaagct     2820

catcaacggc attagggaca agcagagcgg caagaccatc ctggatttcc tgaagtccga     2880

cggcttcgcc aaccgaaact tcatgcagct gattcacgat gacagcttga ccttcaagga     2940

ggacatccag aaggcccagg ttagcggcca gggcgactcc ctgcacgaac atattgcaaa     3000

cctggcaggc tcccctgcga tcaagaaggg catactgcag accgttaagg ttgtggacga     3060

attggtcaag gtcatgggca ggcacaagcc cgaaaacata gttatagaga tggccagaga     3120

gaaccagacc acccaaaagg gccagaagaa cagccgggag cgcatgaaaa ggatcgagga     3180

gggtatcaag gaactcggaa gccagatcct caaagagcac cccgtggaga atacccagct     3240

ccagaacgag aagctgtacc tgtactacct gcagaacggc agggacatgt acgttgacca     3300

ggagttggac atcaacaggc tttcagacta tgacgtggat cacatagtgc cccagagctt     3360

tcttaaagac gatagcatcg acaacaaggt cctgacccgc tccgacaaaa acaggggcaa     3420

aagcgacaac gtgccaagcg aagaggtggt taaaaagatg aagaactact ggaggcaact     3480

gctcaacgcg aaattgatca cccagagaaa gttcgataac ctgaccaagg ccgagagggg     3540

cggactctcc gaacttgaca aagcgggctt cataaagagg cagctggtcg agacccgaca     3600

gatcacgaag cacgtggccc aaatcctcga cagcagaatg aataccaagt acgatgagaa     3660

tgacaaactc atcagggaag tgaaagtgat taccctgaag agcaagttgg tgtccgactt     3720

tcgcaaagat ttccagttct acaaggtgag ggagatcaac aactaccacc atgcccacga     3780

cgcatacctg aacgccgtgg tcggcaccgc cctgattaag aagtatccaa agctggagtc     3840

cgaatttgtc tacggcgact acaaagttta cgatgtgagg aagatgatcg ctaagagcga     3900

acaggagatc ggcaaggcca ccgctaagta tttcttctac agcaacatca tgaacttttt     3960

caagaccgag atcacacttg ccaacggcga aatcaggaag aggccgctta tcgagaccaa     4020

cggtgagacc ggcgagatcg tgtgggacaa gggcagggac ttcgccaccg tgaggaaagt     4080

cctgagcatg ccccaggtga atattgtgaa aaaaactgag gtgcagacag gcggctttag     4140

caaggaatcc atcctgccca agaggaacag cgacaagctg atcgcccgga agaaggactg     4200

ggaccctaag aagtatggag gcttcgacag ccccaccgta gcctacagcg tgctggtggt     4260

cgcgaaggta gagaagggga agagcaagaa actgaagagc gtgaaggagc tgctcggcat     4320

aaccatcatg gagaggtcca gctttgagaa gaaccccatt gactttttgg aagccaaggg     4380

ctacaaagag gtcaaaaagg acctgatcat caaactcccc aagtactccc tgtttgaatt     4440

ggagaacggc agaaagagga tgctggcgag cgctggggaa ctgcaaaagg gcaacgaact     4500

ggcgctgccc agcaagtacg tgaattttct gtacctggcg tcccactacg aaaagctgaa     4560

aggcagcccc gaggacaacg agcagaagca gctgttcgtg gagcagcaca agcattacct     4620

ggacgagata atcgagcaaa tcagcgagtt cagcaagagg gtgattctgg ccgacgcgaa     4680

cctggataag gtcctcagcg cctacaacaa gcaccgagac aaacccatca gggagcaggc     4740

cgagaatatc atacacctgt tcaccctgac aaatctgggc gcacctgcgg cattcaaata     4800

cttcgatacc accatcgaca ggaaaaggta cactagcact aaggaggtgc tggatgccac     4860

cttgatccac cagtccatta ccggcctgta tgagaccagg atcgacctga gccagcttgg     4920

aggcgactct agggcggacc caaaaaagaa aaggaaggtg gaattctcta gaggcagtgg     4980

agagggcaga ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg gcccaatgaa     5040

ccggggagtc ccttttaggc acttgcttct ggtgctgcaa ctggcgctcc tcccagcagc     5100

cactcaggga aagaaagtgg tgctgggcaa aaaaggggat acagtggaac tgacctgtac     5160

agcttcccag aagaagagca tacaattcca ctggaaaaac tccaaccaga taaagattct     5220

gggaaatcag ggctccttct taactaaagg tccatccaag ctgaatgatc gcgctgactc     5280

aagaagaagc ctttgggacc aaggaaactt ccccctgatc atcaagaatc ttaagataga     5340

agactcagat acttacatct gtgaagtgga ggaccagaag gaggaggtgc aattgctagt     5400

gttcggattg actgccaact ctgacaccca cctgcttcag gggcagagcc tgaccctgac     5460

cttggagagc ccccctggta gtagcccctc agtgcaatgt aggagtccaa ggggtaaaaa     5520

catacagggg gggaagaccc tctccgtgtc tcagctggag ctccaggata gtggcacctg     5580

gacatgcact gtcttgcaga accagaagaa ggtggagttc aaaatagaca tcgtggtgct     5640

agctttccag aaggcctcca gcatagtcta taagaaagag ggggaacagg tggagttctc     5700

cttcccactc gcctttacag ttgaaaagct gacgggcagt ggcgagctgt ggtggcaggc     5760

ggagagggct tcctcctcca agtcttggat cacctttgac ctgaagaaca aggaagtgtc     5820

tgtaaaacgg gttacccagg accctaagct ccagatgggc aagaagctcc cgctccacct     5880

caccctgccc caggccttgc ctcagtatgc tggctctgga aacctcaccc tggcccttga     5940

agcgaaaaca ggaaagttgc atcaggaagt gaacctggtg gtgatgagag ccactcagct     6000

ccagaaaaat ttgacctgtg aggtgtgggg acccacctcc cctaagctga tgctgagctt     6060

gaaactggag aacaaggagg caaaggtctc gaagcgggag aaggcggtgt gggtgctgaa     6120

ccctgaggcg gggatgtggc agtgtctgct gagtgactcg ggacaggtcc tgctggaatc     6180

caacatcaag gttctgccca catggtcgac cccggtgcag ccaatggccc tgattgtgct     6240

ggggggcgtc gccggcctcc tgcttttcat tgggctaggc atcttcttct gtgtcaggtg     6300

ccggcacacc ggttagtaat gagtttaaac gggggaggct aactgaaaca cggaaggaga     6360

caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacgggt     6420

gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc     6480

ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc     6540

ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata     6600

gcagatctgc gcagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta     6660

agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg     6720

cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa     6780

gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc     6840

aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt     6900

cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca     6960

acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc     7020

tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt aaggtcgggc     7080

aggaagaggg cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta     7140

gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac     7200

gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact     7260

atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga     7320

aaggacgaaa caccgggggc cactagggac aggatgtttt agagctagaa atagcaagtt     7380

aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttcta     7440

gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt     7500

gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag     7560

cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt     7620

tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag     7680

gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg     7740

ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat     7800

caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta     7860

aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa     7920

atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc     7980

cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt     8040

ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca     8100

gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg     8160

accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat     8220

cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta     8280

cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct     8340

gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac     8400

aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa     8460

aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa     8520

actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt     8580

taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca     8640

gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca     8700

tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc     8760

ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa     8820

accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc     8880

agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca     8940

acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat     9000

tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag     9060

cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac     9120

tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt     9180

ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt     9240

gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc     9300

tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat     9360

ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca     9420

gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga     9480

cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg     9540

gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg     9600

ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga     9660

tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct     9720

gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac     9780

aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agg                       9823


<210>  99
<211>  3296
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: pAM3514

<400>  99
ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac       60

agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa      120

ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca      180

caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc      240

gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttacccgata      300

cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta      360

tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca      420

gcccgaccgc tgcgccttat ccggtaacta tcgtcttaag tccaacccgg taagacacga      480

cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg      540

tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg      600

tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg      660

caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag      720

aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa      780

cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat      840

ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaactggtcg      900

catgcttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca      960

tccatagttg cctgactgcc cgtcgtgtag ataactacga tacgggaggg cttaccatct     1020

ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca     1080

ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc     1140

atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg     1200

cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct     1260

tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa     1320

aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta     1380

tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc     1440

ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg     1500

agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa     1560

gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg     1620

agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc     1680

accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg     1740

gcgacacgga aatgttgaat actcatcaat tgcctttttc aatattattg aagcatttat     1800

cagggttatt gtctcatgag cggttacata tttgaatgta tttagaaaaa taaacaaata     1860

ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc     1920

atgacattaa cctataaaaa taggcgtatc acgaggccct ttcatctcgc gcgtttcggt     1980

gatgacggtg aaaacctctg acacatgcag ctcccggaga cagtcacagc ttgtctgtaa     2040

gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg     2100

ggctggtaaa acgacggcca gtattaaccc tcactaaagg gaactcgagg ctcttcagac     2160

ggcacggcca cgcgtttaaa ccgccagttt tccacacgga cacccccctc ctcaccacag     2220

ccctgccagg acggggctgg ctactggcct tatctcacag gtaaaactga cgcacggagg     2280

aacaatataa attggggact agaaaggtga agagccaaag ttagaactca ggaccaactt     2340

attctgattt tgtttttcca aactgcttct cctcttggga agtgtaagga agctgcagca     2400

ccaggatcag tgaaacgcac cagacggccg cgtcagagca gctcaggttc tgggagaggg     2460

tagcgcaggg tggccactga gaaccgggca ggtcacgcat cccccccttc cctcccaccc     2520

cctgccaagc tctccctccc aggatcctct ctggctccat cgtaagcaaa ccttagaggt     2580

tctggcaagg agagagatgg ctccaggaaa tgggggtgtg tcaccagata aggaatctgc     2640

ctaacaggag gtgggggtta gacccaatat caggagacta ggaaggagga ggcctaagga     2700

tggggctttt ctgtcagaat tcactgtggg gtggagggga cagataaaag tacccagaac     2760

cagagccaca ttaaccggcc ctgggaatat aaggtggtcc cagctcgggg acacaggatc     2820

cctggaggca gcaaacatgc tgtcctgaag tggacatagg ggcccgggtt ggaggaagaa     2880

gactagctga gctctcggac ccctggaaga tgccatgaca gggggctgga agagctagca     2940

cagactagag aggtaagggg ggtaggggag ctgcccaaat gaaaggagtg agaggtgacc     3000

cgaatccaca ggagaacggg gtgtccaggc aaagaaagca agaggatgga gaggtggcta     3060

aagccaggga gacggggtac tttggggttg tccagaaaaa cggtgatgat gcaggcctac     3120

aagaagggga ggcgggacgc aagggagaca tccgtcggag aaggccatcc taagaaacga     3180

gagatggcac aggccccaga aggagaagga aaagggaacg gtgtttaaac cccagcgcct     3240

ggcgggtgaa gagcgagctc ccgctgagca ataactagcg tcatagctgt ttcctg         3296


<210>  100
<211>  5068
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: pAM3515

<400>  100
ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac       60

agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa      120

ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca      180

caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc      240

gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttacccgata      300

cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta      360

tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca      420

gcccgaccgc tgcgccttat ccggtaacta tcgtcttaag tccaacccgg taagacacga      480

cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg      540

tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg      600

tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg      660

caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag      720

aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa      780

cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat      840

ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaactggtcg      900

catgcttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca      960

tccatagttg cctgactgcc cgtcgtgtag ataactacga tacgggaggg cttaccatct     1020

ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca     1080

ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc     1140

atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg     1200

cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct     1260

tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa     1320

aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta     1380

tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc     1440

ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg     1500

agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa     1560

gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg     1620

agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc     1680

accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg     1740

gcgacacgga aatgttgaat actcatcaat tgcctttttc aatattattg aagcatttat     1800

cagggttatt gtctcatgag cggttacata tttgaatgta tttagaaaaa taaacaaata     1860

ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc     1920

atgacattaa cctataaaaa taggcgtatc acgaggccct ttcatctcgc gcgtttcggt     1980

gatgacggtg aaaacctctg acacatgcag ctcccggaga cagtcacagc ttgtctgtaa     2040

gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg     2100

ggctggtaaa acgacggcca gtattaaccc tcactaaagg gaactcgagg ctcttcagac     2160

ggcacggcca cgcgtttaaa ccgccataca gtggaactga cctgtacagc ttcccagaag     2220

aagagcatac aattccactg gaaaaactcc aaccagataa agattctggg aaatcagggc     2280

tccttcttaa ctaaaggtcc atccaagctg aatgatcgcg ctgactcaag aagaagcctt     2340

tgggaccaag gaaacttccc cctgatcatc aagaatctta agatagaaga ctcagatact     2400

tacatctgtg aagtggagga ccagaaggag gaggtgcaat tgctagtgtt cggattgact     2460

gccaactctg acacccacct gcttcagggg cagagcctga ccctgacctt ggagagcccc     2520

cctggtagta gcccctcagt gcaatgtagg agtccaaggg gtaaaaacat acaggggggg     2580

aagaccctct ccgtgtctca gctggagctc caggatagtg gcacctggac atgcactgtc     2640

ttgcagaacc agaagaaggt ggagttcaaa atagacatcg tggtgctagc tttccagaag     2700

gcctccagca tagtctataa gaaagagggg gaacaggtgg agttctcctt cccactcgcc     2760

tttacagttg aaaagctgac gggcagtggc gagctgtggt ggcaggcgga gagggcttcc     2820

tcctccaagt cttggatcac ctttgacctg aagaacaagg aagtgtctgt aaaacgggtt     2880

acccaggacc ctaagctcca gatgggcaag aagctcccgc tccacctcac cctgccccag     2940

gccttgcctc agtatgctgg ctctggaaac ctcaccctgg cccttgaagc gaaaacagga     3000

aagttgcatc aggaagtgaa cctggtggtg atgagagcca ctcagctcca gaaaaatttg     3060

acctgtgagg tgtggggacc cacctcccct aagctgatgc tgagcttgaa actggagaac     3120

aaggaggcaa aggtctcgaa gcgggagaag gcggtgtggg tgctgaaccc tgaggcgggg     3180

atgtggcagt gtctgctgag tgactcggga caggtcctgc tggaatccaa catcaaggtt     3240

ctgcccacat ggtcgacccc ggtgcagcca atggccctga ttgtgctggg gggcgtcgcc     3300

ggcctcctgc ttttcattgg gctaggcatc ttcttctgtg tcaggtgccg gcacaccggt     3360

tagtaatgag tttaaacggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg     3420

aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg     3480

ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc     3540

attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg     3600

aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagca gatctgcgca     3660

gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg     3720

tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg     3780

ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg     3840

ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt     3900

agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt     3960

tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta     4020

tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa     4080

atgagctgat ttaacaaaaa tttaacgcga attaattaag gtcgggcagg aagagggcct     4140

atttcccatg attccttcat atttgcatat acgatacaag gctgttagag agataattag     4200

aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata     4260

atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac     4320

cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac     4380

cgggggccac tagggacagg atgttttaga gctagaaata gcaagttaaa ataaggctag     4440

tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttctagta taccgtcgac     4500

ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc     4560

gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta     4620

atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa     4680

cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat     4740

tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg     4800

agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc     4860

aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt     4920

gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag     4980

tcagaggtgg cggtgtttaa accccagcgc ctggcgggtg aagagcgagc tcccgctgag     5040

caataactag cgtcatagct gtttcctg                                        5068


<210>  101
<211>  4224
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: pAM3516

<400>  101
ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac       60

agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa      120

ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca      180

caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc      240

gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttacccgata      300

cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta      360

tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca      420

gcccgaccgc tgcgccttat ccggtaacta tcgtcttaag tccaacccgg taagacacga      480

cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg      540

tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg      600

tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg      660

caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag      720

aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa      780

cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat      840

ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaactggtcg      900

catgcttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca      960

tccatagttg cctgactgcc cgtcgtgtag ataactacga tacgggaggg cttaccatct     1020

ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca     1080

ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc     1140

atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg     1200

cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct     1260

tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa     1320

aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta     1380

tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc     1440

ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg     1500

agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa     1560

gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg     1620

agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc     1680

accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg     1740

gcgacacgga aatgttgaat actcatcaat tgcctttttc aatattattg aagcatttat     1800

cagggttatt gtctcatgag cggttacata tttgaatgta tttagaaaaa taaacaaata     1860

ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc     1920

atgacattaa cctataaaaa taggcgtatc acgaggccct ttcatctcgc gcgtttcggt     1980

gatgacggtg aaaacctctg acacatgcag ctcccggaga cagtcacagc ttgtctgtaa     2040

gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg     2100

ggctggtaaa acgacggcca gtattaaccc tcactaaagg gaactcgagg ctcttcagac     2160

ggcacggcca cgcgtttaaa ccgccataca gtggaactga cctgtacagc ttcccagaag     2220

aagagcatac aattccactg gaaaaactcc aaccagataa agattctggg aaatcagggc     2280

tccttcttaa ctaaaggtcc atccaagctg aatgatcgcg ctgactcaag aagaagcctt     2340

tgggaccaag gaaacttccc cctgatcatc aagaatctta agatagaaga ctcagatact     2400

tacatctgtg aagtggagga ccagaaggag gaggtgcaat tgctagtgtt cggattgact     2460

gccaactctg acacccacct gcttcagggg cagagcctga ccctgacctt ggagagcccc     2520

cctggtagta gcccctcagt gcaatgtagg agtccaaggg gtaaaaacat acaggggggg     2580

aagaccctct ccgtgtctca gctggagctc caggatagtg gcacctggac atgcactgtc     2640

ttgcagaacc agaagaaggt ggagttcaaa atagacatcg tggtgctagc tttccagaag     2700

gcctccagca tagtctataa gaaagagggg gaacaggtgg agttctcctt cccactcgcc     2760

tttacagttg aaaagctgac gggcagtggc gagctgtggt ggcaggcgga gagggcttcc     2820

tcctccaagt cttggatcac ctttgacctg aagaacaagg aagtgtctgt aaaacgggtt     2880

acccaggacc ctaagctcca gatgggcaag aagctcccgc tccacctcac cctgccccag     2940

gccttgcctc agtatgctgg ctctggaaac ctcaccctgg cccttgaagc gaaaacagga     3000

aagttgcatc aggaagtgaa cctggtggtg atgagagcca ctcagctcca gaaaaatttg     3060

acctgtgagg tgtggggacc cacctcccct aagctgatgc tgagcttgaa actggagaac     3120

aaggaggcaa aggtctcgaa gcgggagaag gcggtgtggg tgctgaaccc tgaggcgggg     3180

atgtggcagt gtctgctgag tgactcggga caggtcctgc tggaatccaa catcaaggtt     3240

ctgcccacat ggtcgacccc ggtgcagcca atggccctga ttgtgctggg gggcgtcgcc     3300

ggcctcctgc ttttcattgg gctaggcatc ttcttctgtg tcaggtgccg gcacaccggt     3360

tagtaatgag tttaaacggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg     3420

aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg     3480

ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc     3540

attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg     3600

aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc gtcgacctct     3660

agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc     3720

acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga     3780

gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg     3840

tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg     3900

cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg     3960

gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga     4020

aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg     4080

gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag     4140

aggtggcggt gtttaaaccc cagcgcctgg cgggtgaaga gcgagctccc gctgagcaat     4200

aactagcgtc atagctgttt cctg                                            4224


<210>  102
<211>  9467
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: pAM15068

<400>  102
gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta       60

ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag      120

ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc      180

ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga      240

cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat      300

atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc      360

cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct      420

attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca      480

cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat      540

caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg      600

cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg      660

agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg      720

agcggccgcc accatgggca agcccatccc taaccccctg ttggggctgg acagcaccgc      780

tcccaaaaag aaaaggaagg tgggcattca cggcgtgcct gcggccgaca aaaagtacag      840

catcggcctt gatatcggca ccaatagcgt gggctgggcc gttatcacag acgaatacaa      900

ggtacccagc aagaagttca aggtgctggg gaatacagac aggcactcta tcaagaaaaa      960

ccttatcggg gctctgctgt ttgactcagg cgagaccgcc gaggccacca ggttgaagag     1020

gaccgcaagg cgaaggtaca cccggaggaa gaacaggatc tgctatctgc aggagatctt     1080

cagcaacgag atggccaagg tggacgacag cttcttccac aggctggagg agagcttcct     1140

tgtcgaggag gataagaagc acgaacgaca ccccatcttc ggcaacatag tcgacgaggt     1200

cgcttatcac gagaagtacc ccaccatcta ccacctgcga aagaaattgg tggatagcac     1260

cgataaagcc gacttgcgac ttatctactt ggctctggcg cacatgatta agttcagggg     1320

ccacttcctg atcgagggcg accttaaccc cgacaacagt gacgtagaca aattgttcat     1380

ccagcttgta cagacctata accagctgtt cgaggaaaac cctattaacg ccagcggggt     1440

ggatgcgaag gccatactta gcgccaggct gagcaaaagc aggcgcttgg agaacctgat     1500

agcccagctg cccggtgaaa agaagaacgg cctcttcggt aatctgattg ccctgagcct     1560

gggcctgacc cccaacttca agagcaactt cgacctggca gaagatgcca agctgcagtt     1620

gagtaaggac acctatgacg acgacttgga caatctgctc gcccaaatcg gcgaccagta     1680

cgctgacctg ttcctcgccg ccaagaacct ttctgacgca atcctgctta gcgatatcct     1740

tagggtgaac acagagatca ccaaggcccc cctgagcgcc agcatgatca agaggtacga     1800

cgagcaccat caggacctga cccttctgaa ggccctggtg aggcagcaac tgcccgagaa     1860

gtacaaggag atctttttcg accagagcaa gaacggctac gccggctaca tcgacggcgg     1920

agccagccaa gaggagttct acaagttcat caagcccatc ctggagaaga tggatggcac     1980

cgaggagctg ctggtgaagc tgaacaggga agatttgctc cggaagcaga ggacctttga     2040

caacggtagc atcccccacc agatccacct gggcgagctg cacgcaatac tgaggcgaca     2100

ggaggatttc taccccttcc tcaaggacaa tagggagaaa atcgaaaaga ttctgacctt     2160

caggatcccc tactacgtgg gccctcttgc caggggcaac agccgattcg cttggatgac     2220

aagaaagagc gaggagacca tcaccccctg gaacttcgag gaagtggtgg acaaaggagc     2280

aagcgcgcag tctttcatcg aacggatgac caatttcgac aaaaacctgc ctaacgagaa     2340

ggtgctgccc aagcacagcc tgctttacga gtacttcacc gtgtacaacg agctcaccaa     2400

ggtgaaatat gtgaccgagg gcatgcgaaa acccgctttc ctgagcggcg agcagaagaa     2460

ggccatcgtg gacctgctgt tcaagaccaa caggaaggtg accgtgaagc agctgaagga     2520

ggactacttc aagaagatcg agtgctttga tagcgtggaa ataagcggcg tggaggacag     2580

gttcaacgcc agcctgggca cctaccacga cttgttgaag ataatcaaag acaaggattt     2640

cctggataat gaggagaacg aggatatact cgaggacatc gtgctgactt tgaccctgtt     2700

tgaggaccga gagatgattg aagaaaggct caaaacctac gcccacctgt tcgacgacaa     2760

agtgatgaaa caactgaaga gacgaagata caccggctgg ggcagactgt ccaggaagct     2820

catcaacggc attagggaca agcagagcgg caagaccatc ctggatttcc tgaagtccga     2880

cggcttcgcc aaccgaaact tcatgcagct gattcacgat gacagcttga ccttcaagga     2940

ggacatccag aaggcccagg ttagcggcca gggcgactcc ctgcacgaac atattgcaaa     3000

cctggcaggc tcccctgcga tcaagaaggg catactgcag accgttaagg ttgtggacga     3060

attggtcaag gtcatgggca ggcacaagcc cgaaaacata gttatagaga tggccagaga     3120

gaaccagacc acccaaaagg gccagaagaa cagccgggag cgcatgaaaa ggatcgagga     3180

gggtatcaag gaactcggaa gccagatcct caaagagcac cccgtggaga atacccagct     3240

ccagaacgag aagctgtacc tgtactacct gcagaacggc agggacatgt acgttgacca     3300

ggagttggac atcaacaggc tttcagacta tgacgtggat cacatagtgc cccagagctt     3360

tcttaaagac gatagcatcg acaacaaggt cctgacccgc tccgacaaaa acaggggcaa     3420

aagcgacaac gtgccaagcg aagaggtggt taaaaagatg aagaactact ggaggcaact     3480

gctcaacgcg aaattgatca cccagagaaa gttcgataac ctgaccaagg ccgagagggg     3540

cggactctcc gaacttgaca aagcgggctt cataaagagg cagctggtcg agacccgaca     3600

gatcacgaag cacgtggccc aaatcctcga cagcagaatg aataccaagt acgatgagaa     3660

tgacaaactc atcagggaag tgaaagtgat taccctgaag agcaagttgg tgtccgactt     3720

tcgcaaagat ttccagttct acaaggtgag ggagatcaac aactaccacc atgcccacga     3780

cgcatacctg aacgccgtgg tcggcaccgc cctgattaag aagtatccaa agctggagtc     3840

cgaatttgtc tacggcgact acaaagttta cgatgtgagg aagatgatcg ctaagagcga     3900

acaggagatc ggcaaggcca ccgctaagta tttcttctac agcaacatca tgaacttttt     3960

caagaccgag atcacacttg ccaacggcga aatcaggaag aggccgctta tcgagaccaa     4020

cggtgagacc ggcgagatcg tgtgggacaa gggcagggac ttcgccaccg tgaggaaagt     4080

cctgagcatg ccccaggtga atattgtgaa aaaaactgag gtgcagacag gcggctttag     4140

caaggaatcc atcctgccca agaggaacag cgacaagctg atcgcccgga agaaggactg     4200

ggaccctaag aagtatggag gcttcgacag ccccaccgta gcctacagcg tgctggtggt     4260

cgcgaaggta gagaagggga agagcaagaa actgaagagc gtgaaggagc tgctcggcat     4320

aaccatcatg gagaggtcca gctttgagaa gaaccccatt gactttttgg aagccaaggg     4380

ctacaaagag gtcaaaaagg acctgatcat caaactcccc aagtactccc tgtttgaatt     4440

ggagaacggc agaaagagga tgctggcgag cgctggggaa ctgcaaaagg gcaacgaact     4500

ggcgctgccc agcaagtacg tgaattttct gtacctggcg tcccactacg aaaagctgaa     4560

aggcagcccc gaggacaacg agcagaagca gctgttcgtg gagcagcaca agcattacct     4620

ggacgagata atcgagcaaa tcagcgagtt cagcaagagg gtgattctgg ccgacgcgaa     4680

cctggataag gtcctcagcg cctacaacaa gcaccgagac aaacccatca gggagcaggc     4740

cgagaatatc atacacctgt tcaccctgac aaatctgggc gcacctgcgg cattcaaata     4800

cttcgatacc accatcgaca ggaaaaggta cactagcact aaggaggtgc tggatgccac     4860

cttgatccac cagtccatta ccggcctgta tgagaccagg atcgacctga gccagcttgg     4920

aggcgactct agggcggacc caaaaaagaa aaggaaggtg gaattctcta gaggcagtgg     4980

agagggcaga ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg gcccaatgaa     5040

ccggggagtc ccttttaggc acttgcttct ggtgctgcaa ctggcgctcc tcccagcagc     5100

cactcaggga aagaaagtgg tgctgggcaa aaaaggggat acagtggaac tgacctgtac     5160

agcttcccag aagaagagca tacaattcca ctggaaaaac tccaaccaga taaagattct     5220

gggaaatcag ggctccttct taactaaagg tccatccaag ctgaatgatc gcgctgactc     5280

aagaagaagc ctttgggacc aaggaaactt ccccctgatc atcaagaatc ttaagataga     5340

agactcagat acttacatct gtgaagtgga ggaccagaag gaggaggtgc aattgctagt     5400

gttcggattg actgccaact ctgacaccca cctgcttcag gggcagagcc tgaccctgac     5460

cttggagagc ccccctggta gtagcccctc agtgcaatgt aggagtccaa ggggtaaaaa     5520

catacagggg gggaagaccc tctccgtgtc tcagctggag ctccaggata gtggcacctg     5580

gacatgcact gtcttgcaga accagaagaa ggtggagttc aaaatagaca tcgtggtgct     5640

agctttccag aaggcctcca gcatagtcta taagaaagag ggggaacagg tggagttctc     5700

cttcccactc gcctttacag ttgaaaagct gacgggcagt ggcgagctgt ggtggcaggc     5760

ggagagggct tcctcctcca agtcttggat cacctttgac ctgaagaaca aggaagtgtc     5820

tgtaaaacgg gttacccagg accctaagct ccagatgggc aagaagctcc cgctccacct     5880

caccctgccc caggccttgc ctcagtatgc tggctctgga aacctcaccc tggcccttga     5940

agcgaaaaca ggaaagttgc atcaggaagt gaacctggtg gtgatgagag ccactcagct     6000

ccagaaaaat ttgacctgtg aggtgtgggg acccacctcc cctaagctga tgctgagctt     6060

gaaactggag aacaaggagg caaaggtctc gaagcgggag aaggcggtgt gggtgctgaa     6120

ccctgaggcg gggatgtggc agtgtctgct gagtgactcg ggacaggtcc tgctggaatc     6180

caacatcaag gttctgccca catggtcgac cccggtgcag ccaatggccc tgattgtgct     6240

ggggggcgtc gccggcctcc tgcttttcat tgggctaggc atcttcttct gtgtcaggtg     6300

ccggcacacc ggttagtaat gagtttaaac gggggaggct aactgaaaca cggaaggaga     6360

caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacgggt     6420

gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc     6480

ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc     6540

ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata     6600

gcagatctgc gcagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta     6660

agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg     6720

cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa     6780

gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc     6840

aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt     6900

cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca     6960

acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc     7020

tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt aagcggccgc     7080

gtgcatatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct     7140

gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt     7200

aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc     7260

gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg     7320

agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg     7380

gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca     7440

gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac     7500

cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac     7560

aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg     7620

tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac     7680

ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat     7740

ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag     7800

cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac     7860

ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt     7920

gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt     7980

atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc     8040

aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga     8100

aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac     8160

gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc     8220

cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct     8280

gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca     8340

tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct     8400

ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca     8460

ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc     8520

atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg     8580

cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct     8640

tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa     8700

aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta     8760

tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc     8820

ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg     8880

agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa     8940

gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg     9000

agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc     9060

accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg     9120

gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat     9180

cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata     9240

ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc     9300

ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt     9360

atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta     9420

caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagg                   9467


<210>  103
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CUT1214

<400>  103
ctagcttgat aaatcgatta gcccggg                                           27


<210>  104
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CUT1215

<400>  104
cccgggctaa tcgatttatc aag                                               23


<210>  105
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CUT1216

<400>  105
ggggccacta gggacaggat gtttt                                             25


<210>  106
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CUT1217

<400>  106
atcctgtccc tagtggcccc cggtg                                             25


<210>  107
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CUT1220

<400>  107
gacggcacgg ccacgcgttt aaaccgccat acagtggaac tgacctgtac agc              53


<210>  108
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CUT1221

<400>  108
cccgccaggc gctggggttt aaacaccgcc acctctgact tgagcgtcg                   49


<210>  109
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CUT1224

<400>  109
ggggcttttc tgtcagaatt cactgtgggg tggaggggac                             40


<210>  110
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CUT1227

<400>  110
cccgccaggc gctggggttt aaacaccgtt cccttttcct tctccttctg gg               52


<210>  111
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CUT1252

<400>  111
gctctagcta gaggtcgacg gctatggcag ggcctgcc                               38


<210>  112
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CUT1253

<400>  112
gcggcaggcc ctgccatagc cgtcgacctc tagctagagc ttg                         43


<210>  113
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CUT1254

<400>  113
taagcggccg ctgcagta                                                     18


<210>  114
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CUT1255

<400>  114
tactgcagcg gccgcttaat                                                   20


<210>  115
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CUT1294

<400>  115
cctggcttta gccacctctc                                                   20


<210>  116
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CUT1297

<400>  116
cctggtgaac acctaggacg                                                   20


<210>  117
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RYSE0

<400>  117
gacggcacgg ccacgcgttt aaaccgcc                                          28


<210>  118
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RYSE19

<400>  118
cccgccaggc gctggggttt aaacaccg                                          28


<210>  119
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CUT1226

<400>  119
gacggcacgg ccacgcgttt aaaccgccag ttttccacac ggacaccc                    48


<210>  120
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CUT1223

<400>  120
gaattctgac agaaaagccc catccttagg                                        30


