               SEQUENCE LISTING

<110> DSM IP Assets B.V.

<120> POLYPEPTIDES WITH PERMEASE ACTIVITY

<130> 30121-WO-PCT

<150> EP13198459.3
<151> 2013-12-19

<160> 62

<170> BiSSAP 1.2

<210> 1
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /organism="Artificial Sequence"
      /note="primer 5034-kanf"
      /mol_type="unassigned DNA"

<400> 1
aagcttgcct cgtccccgcc                                                20


<210> 2
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /organism="Artificial Sequence"
      /note="primer 5035-kanr"
      /mol_type="unassigned DNA"

<400> 2
gtcgacactg gatggcggcg                                                20


<210> 3
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..60
<223> /organism="Artificial Sequence"
      /note="primer 5116-lf2"
      /mol_type="unassigned DNA"

<400> 3
attctagtaa cggccgccag tgtgctggaa ttcgccctta agcttgcctc gtccccgccg     60


<210> 4
<211> 56
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..56
<223> /organism="Artificial Sequence"
      /note="primer 5118-lr2"
      /mol_type="unassigned DNA"

<400> 4
catacattat acgaagttat gcgcgctcta gatatcgtcg acactggatg gcggcg         56


<210> 5
<211> 65
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..65
<223> /organism="Artificial Sequence"
      /note="primer 5115-lf1"
      /mol_type="unassigned DNA"

<400> 5
atccggacgt acgtataact tcgtatagca tacattatac gaagttattc tagtaacggc     60

cgcca                                                                 65


<210> 6
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..60
<223> /organism="Artificial Sequence"
      /note="primer 5117-lr1"
      /mol_type="unassigned DNA"

<400> 6
tcatgacgtc tcgaggccta taacttcgta tagcatacat tatacgaagt tatgcgcgct     60


<210> 7
<211> 5082
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..5082
<223> /organism="Artificial Sequence"
      /note="pRN201; TOPO-BLUNT-loxP-kanMX-loxP"
      /mol_type="unassigned DNA"

<400> 7
agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc       60

acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc      120

tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa      180

ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat      240

ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca      300

ctagtaacgg ccgccagtgt gctggaattc gcccttatcc ggacgtacgt ataacttcgt      360

atagcataca ttatacgaag ttattctagt aacggccgcc agtgtgctgg aattcgccct      420

taagcttgcc tcgtccccgc cgggtcaccc ggccagcgac atggaggccc agaataccct      480

ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc cgtacattta      540

gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc gcacggcgcg      600

aagcaaaaat tacggctcct cgctgcagac ctgcgagcag ggaaacgctc ccctcacaga      660

cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc      720

actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca gttctcacat      780

cacatccgaa cataaacaac catgggtaag gaaaagactc acgtttcgag gccgcgatta      840

aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa      900

tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa      960

catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg     1020

acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg     1080

ttactcacca ctgcgatccc cggcaaaaca gcattccagg tattagaaga atatcctgat     1140

tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct     1200

gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga     1260

atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt     1320

gaacaagtct ggaaagaaat gcataagctt ttgccattct caccggattc agtcgtcact     1380

catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt     1440

gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc     1500

ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat     1560

cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagtactg     1620

acaataaaaa gattcttgtt ttcaagaact tgtcatttgt atagtttttt tatattgtag     1680

ttgttctatt ttaatcaaat gttagcgtga tttatatttt ttttcgcctc gacatcatct     1740

gcccagatgc agttaagtgc gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg     1800

gtcgctatac tgctgtcgat tcgatactaa cgccgccatc cagtgtcgac gatatctaga     1860

gcgcgcataa cttcgtataa tgtatgctat acgaagttat aggcctcgag acgtcatgaa     1920

agggcgaatt ctgagatatc catcacactg gcggccgctc gagcatgcat ctagagggcc     1980

caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga     2040

ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag     2100

ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctata     2160

cgtacggcag tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga     2220

tgtacagagt gatattattg acacgccggg gcgacggatg gtgatccccc tggccagtgc     2280

acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga     2340

aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga     2400

agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg     2460

gggaatataa atgtcaggca tgagattatc aaaaaggatc ttcacctaga tccttttcac     2520

gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct actgggctat     2580

ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg     2640

gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc     2700

gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct cgccgccaag     2760

gatctgatgg cgcaggggat caagctctga tcaagagaca ggatgaggat cgtttcgcat     2820

gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg     2880

ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc     2940

gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca     3000

agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct     3060

cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga     3120

tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg     3180

gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat     3240

cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga     3300

gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg     3360

cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg     3420

ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat     3480

agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct     3540

cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga     3600

cgagttcttc tgaattatta acgcttacaa tttcctgatg cggtattttc tccttacgca     3660

tctgtgcggt atttcacacc gcatacaggt ggcacttttc ggggaaatgt gcgcggaacc     3720

cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc     3780

tgataaatgc ttcaataata gcacgtgagg agggccacca tggccaagtt gaccagtgcc     3840

gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc     3900

gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc     3960

ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg     4020

gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg     4080

gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc     4140

ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg     4200

ctaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg     4260

accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc     4320

aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa     4380

ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag     4440

gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta     4500

ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta     4560

ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag     4620

ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg     4680

gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg     4740

cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag     4800

cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc     4860

cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa     4920

aacgccagca acgcggcctt tttacggttc ctgggctttt gctggccttt tgctcacatg     4980

ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct     5040

gataccgctc gccgcagccg aacgaccgag cgcagcgagt ca                        5082


<210> 8
<211> 5352
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..5352
<223> /organism="Artificial Sequence"
      /note="pRN251; TOPO-BLUNT-loxP-hphMX-loxP"
      /mol_type="unassigned DNA"

<400> 8
agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc       60

acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc      120

tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa      180

ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat      240

ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca      300

ctagtaacgg ccgccagtgt gctggaattc gccctttcat gacgtctcga ggcctataac      360

ttcgtatagc atacattata cgaagttatg cgcgctctag atatcgtcga gcggccgcca      420

gtgtgatgga tatcatcgat gaattcgagc tcgttttcga cactggatgg cggcgttagt      480

atcgaatcga cagcagtata gcgaccagca ttcacatacg attgacgcat gatattactt      540

tctgcgcact taacttcgca tctgggcaga tgatgtcgag gcgaaaaaaa atataaatca      600

cgctaacatt tgattaaaat agaacaacta caatataaaa aaactataca aatgacaagt      660

tcttgaaaac aagaatcttt ttattgtcag tactgattat tcctttgccc tcggacgagt      720

gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc      780

cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc      840

gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata      900

gagttggtca agaccaatgc ggagcatata cgcccggagc cgcggcgatc ctgcaagctc      960

cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca     1020

gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat     1080

gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac     1140

gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc     1200

gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc     1260

aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg     1320

gccgaacccg ctcgtctggc taagatcggc cgcagcgatc gcatccatgg cctccgcgac     1380

cggctgcaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtgc     1440

acggcgggag atgcaatagg tcaggctctc gctgaattcc ccaatgtcaa gcacttccgg     1500

aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc     1560

ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg     1620

agattcttcg ccctccgaga gctgcatcag gtcggagacg ctgtcgaact tttcgatcag     1680

aaacttctcg acagacgtcg cggtgagttc aggcttttta cccatggttg tttatgttcg     1740

gatgtgatgt gagaactgta tcctagcaag attttaaaag gaagtatatg aaagaagaac     1800

ctcagtggca aatcctaacc ttttatattt ctctacaggg gcgcggcgtg gggacaattc     1860

acgcgtctgt gaggggagcg tttccctgct cgcaggtctg cagcgaggag ccgtaatttt     1920

tgcttcgcgc cgtgcggcca tcaaaatgta tggatgcaaa tgattataca tggggatgta     1980

tgggctaaat gtacgggcga cagtcacatc atgcccctga gctgcgcacg tcaagactgt     2040

caaggagggt attctgggcc tccatgtcgc tggccgggtg acccggcggg gacgaggcaa     2100

gcttaagggc gaattccagc acactggcgg ccgttactag aataacttcg tataatgtat     2160

gctatacgaa gttatacgta cgtccggata agggcgaatt ctgagatatc catcacactg     2220

gcggccgctc gagcatgcat ctagagggcc caattcgccc tatagtgagt cgtattacaa     2280

ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa     2340

tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga     2400

tcgcccttcc caacagttgc gcagcctata cgtacggcag tttaaggttt acacctataa     2460

aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgccggg     2520

gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga     2580

actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc     2640

cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga     2700

catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggca tgagattatc     2760

aaaaaggatc ttcacctaga tccttttcac gtagaaagcc agtccgcaga aacggtgctg     2820

accccggatg aatgtcagct actgggctat ctggacaagg gaaaacgcaa gcgcaaagag     2880

aaagcaggta gcttgcagtg ggcttacatg gcgatagcta gactgggcgg ttttatggac     2940

agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa     3000

agtaaactgg atggctttct cgccgccaag gatctgatgg cgcaggggat caagctctga     3060

tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc     3120

tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg     3180

ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac     3240

cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc     3300

cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg     3360

gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga     3420

gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg     3480

cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg     3540

tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt     3600

cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc     3660

ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg     3720

gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga     3780

gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc     3840

gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaattatta acgcttacaa     3900

tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacaggt     3960

ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca     4020

aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata gcacgtgagg     4080

agggccacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc     4140

ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac     4200

ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg     4260

gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag     4320

tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc     4380

ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac     4440

ttcgtggccg aggagcagga ctgacacgtg ctaaaacttc atttttaatt taaaaggatc     4500

taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc     4560

cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg     4620

cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg     4680

gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca     4740

aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg     4800

cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg     4860

tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga     4920

acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac     4980

ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat     5040

ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc     5100

tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga     5160

tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc     5220

ctgggctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg     5280

gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag     5340

cgcagcgagt ca                                                         5352


<210> 9
<211> 4847
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..4847
<223> /organism="Artificial Sequence"
      /note="pRN365; TOPO-BLUNT-loxP-natMX-loxP"
      /mol_type="unassigned DNA"

<400> 9
agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc       60

acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc      120

tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa      180

ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat      240

ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca      300

ctagtaacgg ccgccagtgt gctggaattc gcccttatcc ggacgtacgt ataacttcgt      360

atagcataca ttatacgaag ttattctagt aacggccgcc agtgtgctgg aattcgccct      420

taagcttgcc tcgtccccgc cgggtcaccc ggccagcgac atggaggccc agaataccct      480

ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc cgtacattta      540

gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc gcacggcgcg      600

aagcaaaaat tacggctcct cgctgcagac ctgcgagcag ggaaacgctc ccctcacaga      660

cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc      720

actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca gttctcacat      780

cacatccgaa cataaacaac catgtaaaat gaccactctt gacgacacgg cttaccggta      840

ccgcaccagt gtcccggggg acgccgaggc catcgaggca ctggatgggt ccttcaccac      900

cgacaccgtc ttccgcgtca ccgccaccgg ggacggcttc accctgcggg aggtgccggt      960

ggacccgccc ctgaccaagg tgttccccga cgacgaatcg gacgacgaat cggacgccgg     1020

ggaggacggc gacccggact cccggacgtt cgtcgcgtac ggggacgacg gcgacctggc     1080

gggcttcgtg gtcgtctcgt actccggctg gaaccgccgg ctgaccgtcg aggacatcga     1140

ggtcgccccg gagcaccggg ggcacggggt cgggcgcgcg ttgatggggc tcgcgacgga     1200

gttcgcccgc gagcggggcg ccgggcacct ctggctggag gtcaccaacg tcaacgcacc     1260

ggcgatccac gcgtaccggc ggatggggtt caccctctgc ggcctggaca ccgccctgta     1320

cgacggcacc gcctcggacg gcgagcaggc gctctacatg agcatgccct gcccctagta     1380

ctgacaataa aaagattctt gttttcaaga acttgtcatt tgtatagttt ttttatattg     1440

tagttgttct attttaatca aatgttagcg tgatttatat tttttttcgc ctcgacatca     1500

tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa     1560

tgctggtcgc tatactgctg tcgattcgat actaacgccg ccatccagtg tcgacgatat     1620

ctagagcgcg cataacttcg tataatgtat gctatacgaa gttataggcc tcgagacgtc     1680

atgaaagggc gaattctgag atatccatca cactggcggc cgctcgagca tgcatctaga     1740

gggcccaatt cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt     1800

cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc     1860

gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc     1920

ctatacgtac ggcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt     1980

gtggatgtac agagtgatat tattgacacg ccggggcgac ggatggtgat ccccctggcc     2040

agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg     2100

gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg     2160

gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg     2220

ttctggggaa tataaatgtc aggcatgaga ttatcaaaaa ggatcttcac ctagatcctt     2280

ttcacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg     2340

gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt     2400

acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct     2460

ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttctcgccg     2520

ccaaggatct gatggcgcag gggatcaagc tctgatcaag agacaggatg aggatcgttt     2580

cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta     2640

ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg     2700

tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa     2760

ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct     2820

gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg     2880

caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca     2940

atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat     3000

cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac     3060

gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc     3120

gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa     3180

aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag     3240

gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc     3300

ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt     3360

cttgacgagt tcttctgaat tattaacgct tacaatttcc tgatgcggta ttttctcctt     3420

acgcatctgt gcggtatttc acaccgcata caggtggcac ttttcgggga aatgtgcgcg     3480

gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat     3540

aaccctgata aatgcttcaa taatagcacg tgaggagggc caccatggcc aagttgacca     3600

gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc     3660

ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg     3720

tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg     3780

tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact     3840

tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt     3900

tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac     3960

acgtgctaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc     4020

tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa     4080

agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa     4140

aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc     4200

cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt     4260

agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc     4320

tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac     4380

gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca     4440

gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg     4500

ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag     4560

gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt     4620

ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat     4680

ggaaaaacgc cagcaacgcg gcctttttac ggttcctggg cttttgctgg ccttttgctc     4740

acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt     4800

gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtca                   4847


<210> 10
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..32
<223> /organism="Artificial Sequence"
      /note="primer 115-natf"
      /mol_type="unassigned DNA"

<400> 10
acatgtaaaa tgaccactct tgacgacacg gc                                   32


<210> 11
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..25
<223> /organism="Artificial Sequence"
      /note="primer 116-natr"
      /mol_type="unassigned DNA"

<400> 11
cagtactagg ggccagggca tgctc                                          25


<210> 12
<211> 4254
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..4254
<223> /organism="Artificial Sequence"
      /note="pRN447; TOPO-BLUNT-loxP-zeoMX-loxP"
      /mol_type="unassigned DNA"

<400> 12
agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc       60

acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc      120

tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa      180

ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat      240

ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca      300

ctagtaacgg ccgccagtgt gctggaattc gcccttatcc ggacgtacgt ataacttcgt      360

atagcataca ttatacgaag ttattctagt aacggccgcc agtgtgctgg aattcgccct      420

taagcttgcc tcgtccccgc cgggtcaccc ggccagcgac atggaggccc agaataccct      480

ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc cgtacattta      540

gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc gcacggcgcg      600

aagcaaaaat tacggctcct cgctgcagac ctgcgagcag ggaaacgctc ccctcacaga      660

cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc      720

actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca gttctcacat      780

cacatccgaa cataaacaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg      840

cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc      900

gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc      960

caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag     1020

ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc     1080

atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc     1140

aactgcgtgc acttcgtggc cgaggagcag gactgacaca ctgacaataa aaagattctt     1200

gttttcaaga acttgtcatt tgtatagttt ttttatattg tagttgttct attttaatca     1260

aatgttagcg tgatttatat tttttttcgc ctcgacatca tctgcccaga tgcgaagtta     1320

agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa tgctggtcgc tatactgctg     1380

tcgattcgat actaacgccg ccatccagtg tcgacgatat ctagagcgcg cataacttcg     1440

tataatgtat gctatacgaa gttataggcc tcgagacgtc atgaaagggc gaattctgag     1500

atatccatca cactggcggc cgctcgagca tgcatctaga gggcccaatt cgccctatag     1560

tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg     1620

cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga     1680

agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctatacgtac ggcagtttaa     1740

ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat     1800

tattgacacg ccggggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga     1860

taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat     1920

gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag     1980

ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc     2040

aggcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttcacgtaga aagccagtcc     2100

gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga caagggaaaa     2160

cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggcgat agctagactg     2220

ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct ctggtaaggt     2280

tgggaagccc tgcaaagtaa actggatggc tttctcgccg ccaaggatct gatggcgcag     2340

gggatcaagc tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg     2400

attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca     2460

acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt     2520

tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg     2580

gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga     2640

agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca     2700

ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct     2760

tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac     2820

tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc     2880

gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt     2940

gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt     3000

catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg     3060

tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat     3120

cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaat     3180

tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc     3240

acaccgcata caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt     3300

ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa     3360

taatagcacg tgctaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt     3420

gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc     3480

gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg     3540

caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact     3600

ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg     3660

tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg     3720

ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac     3780

tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca     3840

cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga     3900

gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc     3960

ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct     4020

gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg     4080

agcctatgga aaaacgccag caacgcggcc tttttacggt tcctgggctt ttgctggcct     4140

tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc     4200

tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtca           4254


<210> 13
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..45
<223> /organism="Artificial Sequence"
      /note="primer 28-H3f"
      /mol_type="unassigned DNA"

<400> 13
tgtacatccg gaattctaga ttggtgagcg ctaggagtca ctgcc                     45


<210> 14
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..32
<223> /organism="Artificial Sequence"
      /note="primer 29-H3r"
      /mol_type="unassigned DNA"

<400> 14
ctcgagtatt tcacaccgca tatgatccgt cg                                   32


<210> 15
<211> 6000
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..6000
<223> /organism="Artificial Sequence"
      /note="pRN247 (TOPO- BLUNT-HIS3::loxPkanMXloxP)"
      /mol_type="unassigned DNA"

<400> 15
ggccgccaga tcttccggat ggctcgagtt tttcagcaag atcgcgagta tttcacaccg       60

catatgatcc gtcgagttca agagaaaaaa aaagaaaaag caaaaagaaa aaaggaaagc      120

gcgcctcgtt cagaatgaca cgtatagaat gatgcattac cttgtcatct tcagtatcat      180

actgttcgta tacatactta ctgacattca taggtataca tatatacaca tgtatatata      240

tcgtatgctg cagctttaaa taatcggtgt cactacataa gaacaccttt ggtggaggga      300

acatcgttgg taccattggg cgaggtggct tctcttatgg caaccgcaag agccttgaac      360

gcactctcac tacggtgatg atcattcttg cctcgcagac aatcaacgtg gagggtaatt      420

ctgctagcct ctgcaaagct ttcaagaaaa tgcgggatca tctcgcaaga gagatctcct      480

actttctccc tttgcaaacc aagttcgaca actgcgtacg tataacttcg tatagcatac      540

attatacgaa gttattctag taacggccgc cagtgtgctg gaattcgccc ttaagcttgc      600

ctcgtccccg ccgggtcacc cggccagcga catggaggcc cagaataccc tccttgacag      660

tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc ccgtacattt agcccataca      720

tccccatgta taatcatttg catccataca ttttgatggc cgcacggcgc gaagcaaaaa      780

ttacggctcc tcgctgcaga cctgcgagca gggaaacgct cccctcacag acgcgttgaa      840

ttgtccccac gccgcgcccc tgtagagaaa tataaaaggt taggatttgc cactgaggtt      900

cttctttcat atacttcctt ttaaaatctt gctaggatac agttctcaca tcacatccga      960

acataaacaa ccatgggtaa ggaaaagact cacgtttcga ggccgcgatt aaattccaac     1020

atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg     1080

acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa     1140

ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt     1200

atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc     1260

actgcgatcc ccggcaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa     1320

aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat     1380

tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac     1440

ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc     1500

tggaaagaaa tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat     1560

ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga     1620

cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag     1680

ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg     1740

aataaattgc agtttcattt gatgctcgat gagtttttct aatcagtact gacaataaaa     1800

agattcttgt tttcaagaac ttgtcatttg tatagttttt ttatattgta gttgttctat     1860

tttaatcaaa tgttagcgtg atttatattt tttttcgcct cgacatcatc tgcccagatg     1920

cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta     1980

tactgctgtc gattcgatac taacgccgcc atccagtgtc gacgatatct agagcgcgca     2040

taacttcgta taatgtatgc tatacgaagt tataggaaag agatcgcaat ctgaatcttg     2100

gtttcatttg taatacgctt tactagggct ttctgctctg tcatctttgc cttcgtttat     2160

cttgcctgct cattttttag tatattcttc gaagaaatca cattacttta tataatgtat     2220

aattcattat gtgataatgc caatcgctaa gaaaaaaaaa gagtcatccg ctaggtggaa     2280

aaaaaaaaat gaaaatcatt accgaggcat aaaaaaatat agagtgtact agaggaggcc     2340

aagagtaata gaaaaagaaa attgcgggaa aggactgtgt tatgacttcc ctgactaatg     2400

ccgtgttcaa acgatacctg gcagtgactc ctagcgctca ccaatctaga attccggatg     2460

tacaatcttt ctagaagatc tcctacaata ttctcagctg cttaagggcg aattctgaga     2520

tatccatcac actggcggcc gctcgagcat gcatctagag ggcccaattc gccctatagt     2580

gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc     2640

gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa     2700

gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tatacgtacg gcagtttaag     2760

gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt     2820

attgacacgc cggggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat     2880

aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg     2940

accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc     3000

caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca     3060

ggcatgagat tatcaaaaag gatcttcacc tagatccttt tcacgtagaa agccagtccg     3120

cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac     3180

gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg     3240

gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt     3300

gggaagccct gcaaagtaaa ctggatggct ttctcgccgc caaggatctg atggcgcagg     3360

ggatcaagct ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga     3420

ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa     3480

cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt     3540

ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg     3600

ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa     3660

gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac     3720

cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt     3780

gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact     3840

cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg     3900

ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg     3960

acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc     4020

atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt     4080

gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc     4140

gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaatt     4200

attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg cggtatttca     4260

caccgcatac aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt     4320

tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat     4380

aatagcacgt gaggagggcc accatggcca agttgaccag tgccgttccg gtgctcaccg     4440

cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact     4500

tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg     4560

tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg     4620

agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg     4680

ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg     4740

gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctaaaa cttcattttt     4800

aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac     4860

gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag     4920

atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg     4980

tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca     5040

gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga     5100

actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca     5160

gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc     5220

agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca     5280

ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa     5340

aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc     5400

cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc     5460

gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg     5520

cctttttacg gttcctgggc ttttgctggc cttttgctca catgttcttt cctgcgttat     5580

cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca     5640

gccgaacgac cgagcgcagc gagtcaagcg cccaatacgc aaaccgcctc tccccgcgcg     5700

ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga     5760

gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat     5820

gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag     5880

ctatgaccat gattacgcca agctatttag gtgacactat agaatactca agctatgcat     5940

caagcttggt accgagctcg gatccactag taacggccgc cagtgtgctg gaattcgccc     6000


<210> 16
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..35
<223> /organism="Artificial Sequence"
      /note="primer 201-Hx2uf"
      /mol_type="unassigned DNA"

<400> 16
gactagtacc ggtgttttca aaacctagca acccc                                35


<210> 17
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..43
<223> /organism="Artificial Sequence"
      /note="primer 202-Hx2ur"
      /mol_type="unassigned DNA"

<400> 17
cgtacgcgtc ttccggaagg gtaccatcag atttcatttg acc                       43


<210> 18
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..40
<223> /organism="Artificial Sequence"
      /note="primer 203-Hx2df"
      /mol_type="unassigned DNA"

<400> 18
gaagacactc gagacgtcct ttgtctgtga aaccaagggc                           40


<210> 19
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..39
<223> /organism="Artificial Sequence"
      /note="primer 204-Hx2dr"
      /mol_type="unassigned DNA"

<400> 19
gtcgacgggc ccttatgttg gtcttgttta gtatggccg                            39


<210> 20
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..48
<223> /organism="Artificial Sequence"
      /note="primer 205-Hx3uf"
      /mol_type="unassigned DNA"

<400> 20
aagcggccgc actagtaccg gtgaaacaac tcaataacga tgtgggac                  48


<210> 21
<211> 38
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..38
<223> /organism="Artificial Sequence"
      /note="primer 206-Hx3urATCCGGACGTCTTCCTCAAGAAATCAGTTTGGGCGACG"
      /mol_type="unassigned DNA"

<400> 21
atccggacgt cttcctcaag aaatcagttt gggcgacg                             38


<210> 22
<211> 44
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..44
<223> /organism="Artificial Sequence"
      /note="primer 210-Hx4df"
      /mol_type="unassigned DNA"

<400> 22
agaagacgct cgagacgtcc cttatgggaa gaaggtgttt tgcc                      44


<210> 23
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..34
<223> /organism="Artificial Sequence"
      /note="primer 211-Hx4dr"
      /mol_type="unassigned DNA"

<400> 23
atggatccta ggggttcttg cagagtaaac tgcg                                 34


<210> 24
<211> 46
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..46
<223> /organism="Artificial Sequence"
      /note="primer 212-Hx5uf"
      /mol_type="unassigned DNA"

<400> 24
aagcggccgc actagtacat gtgaacttga aaacgctcat caaggc                    46


<210> 25
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..43
<223> /organism="Artificial Sequence"
      /note="primer 213-Hx5ur"
      /mol_type="unassigned DNA"

<400> 25
ttcgtacgcg tcttccggag taacatgaaa ccagagtacc acg                       43


<210> 26
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..42
<223> /organism="Artificial Sequence"
      /note="primer 229-Hx7df"
      /mol_type="unassigned DNA"

<400> 26
agaagaccct cgagacgtcc gacgctgaag aaatgactca cg                        42


<210> 27
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..42
<223> /organism="Artificial Sequence"
      /note="primer 230-Hx7dr"
      /mol_type="unassigned DNA"

<400> 27
agtcgacgga tccgtaattt ttcttctttt aagtgacggg cg                        42


<210> 28
<211> 44
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..44
<223> /organism="Artificial Sequence"
      /note="primer 243-Gal2ufn"
      /mol_type="unassigned DNA"

<400> 28
aagcggccgc actagtaccg gtgatctata ttcgaaaggg gcgg                      44


<210> 29
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..43
<223> /organism="Artificial Sequence"
      /note="primer 244-Gal2urn"
      /mol_type="unassigned DNA"

<400> 29
aacgtacgtc cggatcatta gaatactttt gagattgtgc gct                       43


<210> 30
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..43
<223> /organism="Artificial Sequence"
      /note="primer 233-Ga2df"
      /mol_type="unassigned DNA"

<400> 30
agaagaccct cgagacgtct taccttggaa atctgaaggc tgg                       43


<210> 31
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..36
<223> /organism="Artificial Sequence"
      /note="primer 234-Ga2dr"
      /mol_type="unassigned DNA"

<400> 31
gtggatccta ggtaaaacgg tacgagaaaa gctccg                               36


<210> 32
<211> 5959
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..5959
<223> /organism="Artificial Sequence"
      /note="pRN485; TOPO-BLUNT-GAL2::loxPzeoMXloxP"
      /mol_type="unassigned DNA"

<400> 32
agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc       60

acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc      120

tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa      180

ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat      240

ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca      300

ctagtaacgg ccgccagtgt gctggaattc gcccttgtgg atcctaggaa tggatgatgg      360

taaaacggta cgagaaaagc tccgagacgt tgaattgaat attaataggt tgcaggttga      420

tataaaagaa ataaaggaaa tgcttgttac actgataaac aaatgactga aaaaagcaac      480

tcctggatcg ttcgaacatt ctcactccat taattgtatg ttagctcagg aattcaactg      540

gaagaaagtc caggcaagta cctgacttat tttctagagg accccccaga gataagtctg      600

gtgatgtggt cctttaataa tttcataggg acaattcatt ttgcgttttt aattttgctc      660

ggtgaacaaa ggatggcaga gcatgttatc gttttctttt ttttttcctg ttttagagaa      720

aaaggttata tatgtaataa tactctgata tatgtacaca aataataggt ttaggtaagg      780

aatttatata atcgtaagga tatcattgat aagggaaatt tttttttttt tttttcaaac      840

aaatactatg ataattaaaa tgaagaaaaa acgtcagtca tgaaaaatta agagagatga      900

tggagcgtct cacttcaaac gcattattct agcatggcct tgtaccacgg tttgtcgtca      960

tgttgtaaat cctctaaatc gtaattatta cctcttctgg atgaaggaat ccagccttca     1020

gatttccaag gtaagacgtc tcgaggccta taacttcgta tagcatacat tatacgaagt     1080

tatgcgcgct ctagatatcg tcgacactgg atggcggcgt tagtatcgaa tcgacagcag     1140

tatagcgacc agcattcaca tacgattgac gcatgatatt actttctgcg cacttaactt     1200

cgcatctggg cagatgatgt cgaggcgaaa aaaaatataa atcacgctaa catttgatta     1260

aaatagaaca actacaatat aaaaaaacta tacaaatgac aagttcttga aaacaagaat     1320

ctttttattg tcagtgtgtc agtcctgctc ctcggccacg aagtgcacgc agttgccggc     1380

cgggtcgcgc agggcgaact cccgccccca cggctgctcg ccgatctcgg tcatggccgg     1440

cccggaggcg tcccggaagt tcgtggacac gacctccgac cactcggcgt acagctcgtc     1500

caggccgcgc acccacaccc aggccagggt gttgtccggc accacctggt cctggaccgc     1560

gctgatgaac agggtcacgt cgtcccggac cacaccggcg aagtcgtcct ccacgaagtc     1620

ccgggagaac ccgagccggt cggtccagaa ctcgaccgct ccggcgacgt cgcgcgcggt     1680

gagcaccgga acggcactgg tcaacttggc catggttgtt tatgttcgga tgtgatgtga     1740

gaactgtatc ctagcaagat tttaaaagga agtatatgaa agaagaacct cagtggcaaa     1800

tcctaacctt ttatatttct ctacaggggc gcggcgtggg gacaattcaa cgcgtctgtg     1860

aggggagcgt ttccctgctc gcaggtctgc agcgaggagc cgtaattttt gcttcgcgcc     1920

gtgcggccat caaaatgtat ggatgcaaat gattatacat ggggatgtat gggctaaatg     1980

tacgggcgac agtcacatca tgcccctgag ctgcgcacgt caagactgtc aaggagggta     2040

ttctgggcct ccatgtcgct ggccgggtga cccggcgggg acgaggcaag cttaagggcg     2100

aattccagca cactggcggc cgttactaga ataacttcgt ataatgtatg ctatacgaag     2160

ttatacgtac gtccggatca ttagaatact tttgagattg tgcgcttaaa tgggaatctt     2220

tactgagtga agagatcacg tcttcaccag cttggggttg ctgtgaaaca acaggcatat     2280

tgttctcctc aactgccatt atgaaagaat tatttttttt attatgttaa tcttgtgttt     2340

acttaactat tactattctt gatgataatt gaataaggtg cataatgaag agcaattcac     2400

aacaccaaat tttcaatcca attactgatt gtttatatat gtctacaaaa cttatcctat     2460

ctccacattt tagcctgcga aatgtttgtt ttttgaacaa tagctctcca gaatgttgta     2520

taatttaaga atatgtgcac agttaacttt ctagcaggag tataatgcca tttgctcccc     2580

atcttgagat gggaagggct taactaatct cggttcggag tgatccgccc cgatactgcc     2640

ttctgcctta atatcgtcca aggcacatgg acccctgaac ggcgcagata tctccgcacg     2700

gacgaaagac cgccggtgcc ttcctgaggc aaccgcccct ttcgaatata gatcaccggt     2760

actagtgcgg ccgcttaagg gcgaattctg cagatatcca tcacactggc ggccgctcga     2820

gcatgcatct agagggccca attcgcccta tagtgagtcg tattacaatt cactggccgt     2880

cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc     2940

acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca     3000

acagttgcgc agcctatacg tacggcagtt taaggtttac acctataaaa gagagagccg     3060

ttatcgtctg tttgtggatg tacagagtga tattattgac acgccggggc gacggatggt     3120

gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt     3180

ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt     3240

ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc     3300

cattaacctg atgttctggg gaatataaat gtcaggcatg agattatcaa aaaggatctt     3360

cacctagatc cttttcacgt agaaagccag tccgcagaaa cggtgctgac cccggatgaa     3420

tgtcagctac tgggctatct ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc     3480

ttgcagtggg cttacatggc gatagctaga ctgggcggtt ttatggacag caagcgaacc     3540

ggaattgcca gctggggcgc cctctggtaa ggttgggaag ccctgcaaag taaactggat     3600

ggctttctcg ccgccaagga tctgatggcg caggggatca agctctgatc aagagacagg     3660

atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg     3720

ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc     3780

cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg     3840

tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt     3900

tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg     3960

cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat     4020

catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca     4080

ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca     4140

ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa     4200

ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa     4260

tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc     4320

ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga     4380

atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc     4440

cttctatcgc cttcttgacg agttcttctg aattattaac gcttacaatt tcctgatgcg     4500

gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacaggtgg cacttttcgg     4560

ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg     4620

ctcatgagac aataaccctg ataaatgctt caataatagc acgtgaggag ggccaccatg     4680

gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag     4740

ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg     4800

gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac     4860

accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc     4920

gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg     4980

tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag     5040

gagcaggact gacacgtgct aaaacttcat ttttaattta aaaggatcta ggtgaagatc     5100

ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca     5160

gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc     5220

tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta     5280

ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt     5340

ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc     5400

gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg     5460

ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg     5520

tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag     5580

ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc     5640

agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat     5700

agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg     5760

gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct gggcttttgc     5820

tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt     5880

accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca     5940

gtgagcgagg aagcggaag                                                  5959


<210> 33
<211> 6384
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..6384
<223> /organism="Artificial Sequence"
      /note="pRN566; TOPO-BLUNT-HXT367::loxP-hphMX-loxP"
      /mol_type="unassigned DNA"

<400> 33
agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc       60

acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc      120

tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa      180

ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat      240

ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca      300

ctagtaccgg tgaaacaact caataacgat gtgggacatt gggggcccac tcaaaaaatc      360

tggggactat atccccagag aatttctcca gaagagaaga aaagtcaaag ttttttttcg      420

cttgggggtt gcatataaat acaggcgctg ttttatcttc agcatgaata ttccataatt      480

ttacttaata gcttttcata aataatagaa tcacaaacaa aatttacatc tgagttaaac      540

aatcatgaat tcaactccag atttaatatc tccacaaaag tcaagtgaga attcgaatgc      600

tgacctgcct tcgaatagct ctcaggtaat gaacatgcct gaagaaaaag gtgttcaaga      660

tgatttccaa gctgaggccg accaagtact taccaaccca aatacaggta aaggtgcata      720

tgtcactgtg tctatctgtt gtgttatggt tgccttcggt ggtttcgttt tcggttggga      780

tactggtacc atttctggtt tcgtcgccca aactgatttc ttgaggaaga cgtccggacg      840

tacgtataac ttcgtatagc atacattata cgaagttatt ctagtaacgg ccgccagtgt      900

gctggaattc gcccttaagc ttgcctcgtc cccgccgggt cacccggcca gcgacatgga      960

ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg     1020

tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca tacattttga     1080

tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg cagacctgcg agcagggaaa     1140

cgctcccctc acagacgcgt gaattgtccc cacgccgcgc ccctgtagag aaatataaaa     1200

ggttaggatt tgccactgag gttcttcttt catatacttc cttttaaaat cttgctagga     1260

tacagttctc acatcacatc cgaacataaa caaccatggg taaaaagcct gaactcaccg     1320

cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc     1380

tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc     1440

tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg     1500

catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga     1560

cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac     1620

tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta     1680

gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc     1740

gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg     1800

acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact     1860

gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca     1920

atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg     1980

aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct     2040

acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc     2100

gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt     2160

gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac     2220

aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata     2280

gtggaaaccg acgccccagc actcgtccga gggcaaagga ataatcagta ctgacaataa     2340

aaagattctt gttttcaaga acttgtcatt tgtatagttt ttttatattg tagttgttct     2400

attttaatca aatgttagcg tgatttatat tttttttcgc ctcgacatca tctgcccaga     2460

tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa tgctggtcgc     2520

tatactgctg tcgattcgat actaacgccg ccatccagtg tcgaaaacga gctcgaattc     2580

atcgatgata tccatcacac tggcggccgc tcgacgatat ctagagcgcg cataacttcg     2640

tataatgtat gctatacgaa gttataggcc tcgagacgtc cgacgctgaa gaaatgactc     2700

acgatgacaa gccattgtac aagagaatgt tcagcaccaa ataatttgcg aacactttta     2760

ttaattcatg atcacgctct aatttgtgca tttgaaatgt actctaattc taattttata     2820

tttttaatga tatcttgaaa agtaaatacg tttttaatat atacaaaata atacagttta     2880

attttcaagt ttttgatcat ttgttctcag aaagttgagt gggacggaga caaagaaact     2940

ttaaagagaa atgcaaagtg ggaagaagtc agttgtttac cgaccgcact gttattcaca     3000

aatattccaa ttttgcctgc agacccacgt ctacaaattt tggttagttt ggtaaatggt     3060

aaggatatag tagagccttt ttgaaatggg aaatatcttc tttttctgta tcccgcttca     3120

aaaagtgtct aatgagtcag ttatttcttt cttactcatc gcccgtcact taaaagaaga     3180

aaaattacgg atccgtcgac taagggcgaa ttctgcagat atccatcaca ctggcggccg     3240

ctcgagcatg catctagagg gcccaattcg ccctatagtg agtcgtatta caattcactg     3300

gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt     3360

gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct     3420

tcccaacagt tgcgcagcct atacgtacgg cagtttaagg tttacaccta taaaagagag     3480

agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc ggggcgacgg     3540

atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac     3600

ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg     3660

ccggtctccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa     3720

aacgccatta acctgatgtt ctggggaata taaatgtcag gcatgagatt atcaaaaagg     3780

atcttcacct agatcctttt cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg     3840

atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag     3900

gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc     3960

gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac     4020

tggatggctt tctcgccgcc aaggatctga tggcgcaggg gatcaagctc tgatcaagag     4080

acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc     4140

gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat     4200

gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg     4260

tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg     4320

ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta     4380

ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta     4440

tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc     4500

gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc     4560

gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg     4620

ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg     4680

ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt     4740

gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc     4800

ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc     4860

atcgccttct atcgccttct tgacgagttc ttctgaatta ttaacgctta caatttcctg     4920

atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcataca ggtggcactt     4980

ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt     5040

atccgctcat gagacaataa ccctgataaa tgcttcaata atagcacgtg aggagggcca     5100

ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg     5160

tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg     5220

gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg     5280

acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg     5340

aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc     5400

agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg     5460

ccgaggagca ggactgacac gtgctaaaac ttcattttta atttaaaagg atctaggtga     5520

agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag     5580

cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa     5640

tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag     5700

agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg     5760

tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat     5820

acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta     5880

ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg     5940

gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc     6000

gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa     6060

gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc     6120

tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt     6180

caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctgggct     6240

tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc     6300

gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg     6360

agtcagtgag cgaggaagcg gaag                                            6384


<210> 34
<211> 6073
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..6073
<223> /organism="Artificial Sequence"
      /note="pRN569: TOPO-BLUNT-HXT514::loxP-natMX-loxP"
      /mol_type="unassigned DNA"

<400> 34
agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc       60

acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc      120

tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa      180

ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat      240

ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca      300

ctagtaacgg ccgccagtgt gctggaattc gcccttatgg atcctagggg ttcttgcaga      360

gtaaactgcg attcttcaga taaactatcc cgaacatatt gacccacttt caggtaggtc      420

attcaaagct ttttgattga cggtagatca aagttgtgtt ctacgtattt acgtcttttt      480

tgtcgtattt attgatgacc ttttgccatc gttaagtgga gaattcggcc tattcctaac      540

ctcttccaga actttgaaac aaaaaacata attcaaaatt gtagtttgat aaagtattat      600

ctataaacta ttaaatcata aatatatatt tcatagagcg ttaaaaattg aagatcaaat      660

attattttta ttccttgaag gaagtctata ttatttaatt aactgaccta cttttttccg      720

aacatcttct tgtaaaatgg ttgatcatca tgcattagat catcagcgtt gtagtcagta      780

cctctcttgt ttggtggaac ccaagaaggt gatttccatg gcaaaacacc ttcttcccat      840

aagggacgtc tcgaggccta taacttcgta tagcatacat tatacgaagt tatgcgcgct      900

ctagatatcg tcgacactgg atggcggcgt tagtatcgaa tcgacagcag tatagcgacc      960

agcattcaca tacgattgac gcatgatatt actttctgcg cacttaactt cgcatctggg     1020

cagatgatgt cgaggcgaaa aaaaatataa atcacgctaa catttgatta aaatagaaca     1080

actacaatat aaaaaaacta tacaaatgac aagttcttga aaacaagaat ctttttattg     1140

tcagtactag gggcagggca tgctcatgta gagcgcctgc tcgccgtccg aggcggtgcc     1200

gtcgtacagg gcggtgtcca ggccgcagag ggtgaacccc atccgccggt acgcgtggat     1260

cgccggtgcg ttgacgttgg tgacctccag ccagaggtgc ccggcgcccc gctcgcgggc     1320

gaactccgtc gcgagcccca tcaacgcgcg cccgaccccg tgcccccggt gctccggggc     1380

gacctcgatg tcctcgacgg tcagccggcg gttccagccg gagtacgaga cgaccacgaa     1440

gcccgccagg tcgccgtcgt ccccgtacgc gacgaacgtc cgggagtccg ggtcgccgtc     1500

ctccccggcg tccgattcgt cgtccgattc gtcgtcgggg aacaccttgg tcaggggcgg     1560

gtccaccggc acctcccgca gggtgaagcc gtccccggtg gcggtgacgc ggaagacggt     1620

gtcggtggtg aaggacccat ccagtgcctc gatggcctcg gcgtcccccg ggacactggt     1680

gcggtaccgg taagccgtgt cgtcaagagt ggtcatttta catggttgtt tatgttcgga     1740

tgtgatgtga gaactgtatc ctagcaagat tttaaaagga agtatatgaa agaagaacct     1800

cagtggcaaa tcctaacctt ttatatttct ctacaggggc gcggcgtggg gacaattcaa     1860

cgcgtctgtg aggggagcgt ttccctgctc gcaggtctgc agcgaggagc cgtaattttt     1920

gcttcgcgcc gtgcggccat caaaatgtat ggatgcaaat gattatacat ggggatgtat     1980

gggctaaatg tacgggcgac agtcacatca tgcccctgag ctgcgcacgt caagactgtc     2040

aaggagggta ttctgggcct ccatgtcgct ggccgggtga cccggcgggg acgaggcaag     2100

cttaagggcg aattccagca cactggcggc cgttactaga ataacttcgt ataatgtatg     2160

ctatacgaag ttatacgtac gtccggagta acatgaaacc agagtaccac gcaactgctt     2220

aggcgacact tcagaaatta gcataggcgc caaaactgta ataccaccaa cgcccagtcc     2280

tgagatgatt cttccaatga aatattgata ccatttgtca atggaggcga tttggatgat     2340

gatcccaatt gagtaaatga cgacaacagt catcagacca atcttacgtc catacatatc     2400

accgagcttt gacaaaacta tacctccgat agcgcagccg atgttgaaaa tagaaaccat     2460

caaaccggtt ctgacatcgg aaagataggt agtcccgttt gcacgggtgc tgccaaatcg     2520

cctaatgaag tctgtttgcc tgacaaaacc agatatagta ccagtatccc acccaaacac     2580

gaacccacca aaagcaacca tcaaacagca gacggataca aatagtaaat ccgacttcga     2640

tttcttctct agttggttgt caacctcctt ctgaagctct tccagttcgt ctttgggagg     2700

gccttcatgg gaaatgtaac ttgagacggg tttagcttgt gccaaattat cgttgtaacc     2760

ggcggtacca ggagcagtcg agtttcctga cttctcgttg tatgagttag aatttgtgct     2820

cacagtagca gacccttcca aggggccttg atgagcgttt tcaagttcac atgtactagt     2880

gcggccgctt aagggcgaat tctgcagata tccatcacac tggcggccgc tcgagcatgc     2940

atctagaggg cccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt     3000

acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc     3060

ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt     3120

gcgcagccta tacgtacggc agtttaaggt ttacacctat aaaagagaga gccgttatcg     3180

tctgtttgtg gatgtacaga gtgatattat tgacacgccg gggcgacgga tggtgatccc     3240

cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca     3300

tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt     3360

tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa     3420

cctgatgttc tggggaatat aaatgtcagg catgagatta tcaaaaagga tcttcaccta     3480

gatccttttc acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag     3540

ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag     3600

tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt     3660

gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt     3720

ctcgccgcca aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg     3780

atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga     3840

gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt     3900

ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct     3960

gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg     4020

cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt     4080

gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc     4140

tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc     4200

gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga     4260

tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag     4320

catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat     4380

ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg     4440

ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc     4500

tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta     4560

tcgccttctt gacgagttct tctgaattat taacgcttac aatttcctga tgcggtattt     4620

tctccttacg catctgtgcg gtatttcaca ccgcatacag gtggcacttt tcggggaaat     4680

gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg     4740

agacaataac cctgataaat gcttcaataa tagcacgtga ggagggccac catggccaag     4800

ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg     4860

accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg     4920

gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg     4980

gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc     5040

acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg     5100

cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag     5160

gactgacacg tgctaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt     5220

gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc     5280

gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg     5340

caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact     5400

ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg     5460

tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg     5520

ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac     5580

tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca     5640

cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga     5700

gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc     5760

ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct     5820

gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg     5880

agcctatgga aaaacgccag caacgcggcc tttttacggt tcctgggctt ttgctggcct     5940

tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc     6000

tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc     6060

gaggaagcgg aag                                                        6073


<210> 35
<211> 6189
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..6189
<223> /organism="Artificial Sequence"
      /note="pRN635; TOPO-BLUNT-HXT2::loxP-kanMX-loxP"
      /mol_type="unassigned DNA"

<400> 35
agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc       60

acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc      120

tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa      180

ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat      240

ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca      300

ctagtaacgg ccgccagtgt gctggaattc gcccttgact agtaccggtg ttttcaaaac      360

ctagcaaccc ccaccaaact tgtcatcgtt cccggattca caaatgatat aaaaagcgat      420

tacaattcta cattctaacc agatttgaga tttcctcttt ctcaattcct cttatattag      480

attataagaa caacaaatta aattacaaaa agacttataa agcaacataa tgtctgaatt      540

cgctactagc cgcgttgaaa gtggctctca acaaacttct atccactcta ctccgatagt      600

gcagaaatta gagacggatg aatctcctat tcaaaccaaa tctgaataca ctaacgctga      660

actcccagca aagccaatcg ccgcatattg gactgttatc tgtttatgtc taatgattgc      720

atttggtggg tttgtctttg gttgggatac tggtaccatc tctggttttg ttaatcaaac      780

cgatttcaaa agaagatttg gtcaaatgaa atctgatggt accttccgga agacgcgtac      840

gtataacttc gtatagcata cattatacga agttattcta gtaacggccg ccagtgtgct      900

ggaattcgcc cttaagcttg cctcgtcccc gccgggtcac ccggccagcg acatggaggc      960

ccagaatacc ctccttgaca gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg     1020

cccgtacatt tagcccatac atccccatgt ataatcattt gcatccatac attttgatgg     1080

ccgcacggcg cgaagcaaaa attacggctc ctcgctgcag acctgcgagc agggaaacgc     1140

tcccctcaca gacgcgttga attgtcccca cgccgcgccc ctgtagagaa atataaaagg     1200

ttaggatttg ccactgaggt tcttctttca tatacttcct tttaaaatct tgctaggata     1260

cagttctcac atcacatccg aacataaaca accatgggta aggaaaagac tcacgtttcg     1320

aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat     1380

aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag     1440

ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga     1500

ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct     1560

gatgatgcat ggttactcac cactgcgatc cccggcaaaa cagcattcca ggtattagaa     1620

gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg     1680

cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag     1740

gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat     1800

ggctggcctg ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat     1860

tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta     1920

ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc     1980

ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat     2040

ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc     2100

taatcagtac tgacaataaa aagattcttg ttttcaagaa cttgtcattt gtatagtttt     2160

tttatattgt agttgttcta ttttaatcaa atgttagcgt gatttatatt ttttttcgcc     2220

tcgacatcat ctgcccagat gcgaagttaa gtgcgcagaa agtaatatca tgcgtcaatc     2280

gtatgtgaat gctggtcgct atactgctgt cgattcgata ctaacgccgc catccagtgt     2340

cgacgatatc tagagcgcgc ataacttcgt ataatgtatg ctatacgaag ttataggcct     2400

cgagacgtcc tttgtctgtg aaaccaaggg cttaacatta gaggaagtta atgaaatgta     2460

tgttgaaggt gtcaaaccat ggaaatctgg tagctggatc tcaaaagaaa aaagagtttc     2520

cgaggaataa gagattatac ttaaactagc actgattttt ttaaggctaa tggctactaa     2580

tactttaata gatgatcttc atactttttt atttaacgat ttttaatgat gtttttattt     2640

gtaccactca tttatctaga tttttttaat actgatcaaa tcttacggac tcgacgttaa     2700

aaagttccta catacgtctg gtacttgaaa cgctgcttcg aggtattgac actataagaa     2760

tacgatccaa atacttacac cgcatgtaaa aatatgccga caatatgaat acttgttgat     2820

gaatgatatt tgattttaat ccggcaattt acctccttta tataatccaa taattgttga     2880

taattagtgg ttaggttgca gtactaataa gaattaagac aaatattctt ctactatata     2940

aaaggtgcaa acaaaacaca cgccgatcgg ccatactaaa caagaccaac ataagggccc     3000

gtcgacaagg gcgaattctg cagatatcca tcacactggc ggccgctcga gcatgcatct     3060

agagggccca attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa     3120

cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct     3180

ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc     3240

agcctatacg tacggcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg     3300

tttgtggatg tacagagtga tattattgac acgccggggc gacggatggt gatccccctg     3360

gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc     3420

ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc     3480

ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg     3540

atgttctggg gaatataaat gtcaggcatg agattatcaa aaaggatctt cacctagatc     3600

cttttcacgt agaaagccag tccgcagaaa cggtgctgac cccggatgaa tgtcagctac     3660

tgggctatct ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg     3720

cttacatggc gatagctaga ctgggcggtt ttatggacag caagcgaacc ggaattgcca     3780

gctggggcgc cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttctcg     3840

ccgccaagga tctgatggcg caggggatca agctctgatc aagagacagg atgaggatcg     3900

tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg     3960

ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg     4020

ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat     4080

gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca     4140

gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg     4200

gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat     4260

gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa     4320

catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg     4380

gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg     4440

cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg     4500

gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat     4560

caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac     4620

cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc     4680

cttcttgacg agttcttctg aattattaac gcttacaatt tcctgatgcg gtattttctc     4740

cttacgcatc tgtgcggtat ttcacaccgc atacaggtgg cacttttcgg ggaaatgtgc     4800

gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac     4860

aataaccctg ataaatgctt caataatagc acgtgaggag ggccaccatg gccaagttga     4920

ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg     4980

accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg     5040

acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct     5100

gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga     5160

acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg     5220

agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact     5280

gacacgtgct aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata     5340

atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag     5400

aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa     5460

caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt     5520

ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc     5580

cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa     5640

tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa     5700

gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc     5760

ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa     5820

gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa     5880

caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg     5940

ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc     6000

tatggaaaaa cgccagcaac gcggcctttt tacggttcct gggcttttgc tggccttttg     6060

ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg     6120

agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg     6180

aagcggaag                                                             6189


<210> 36
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..24
<223> /organism="Artificial Sequence"
      /note="primer 281-Hx3inr2"
      /mol_type="unassigned DNA"

<400> 36
gctcttttca cggagaaatt cggg                                           24


<210> 37
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..22
<223> /organism="Artificial Sequence"
      /note="primer 323-Hx7inr1"
      /mol_type="unassigned DNA"

<400> 37
gatgagaatc cttggcaacc gc                                             22


<210> 38
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..25
<223> /organism="Artificial Sequence"
      /note="primer Hx4inr2"
      /mol_type="unassigned DNA"

<400> 38
ccatactatt tgtcgactca agcgc                                          25


<210> 39
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..25
<223> /organism="Artificial Sequence"
      /note="primer Hx5inf"
      /mol_type="unassigned DNA"

<400> 39
gggttaatta gttttagggg cacgg                                          25


<210> 40
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..24
<223> /organism="Artificial Sequence"
      /note="primer 324-Ga2inf1"
      /mol_type="unassigned DNA"

<400> 40
tcaattcgga aagcttcctt ccgg                                           24


<210> 41
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..23
<223> /organism="Artificial Sequence"
      /note="primer 325-Ga2inr1"
      /mol_type="unassigned DNA"

<400> 41
cagtgatagt ttggttcgag cgg                                            23


<210> 42
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..23
<223> /organism="Artificial Sequence"
      /note="primer 289-Hx2inf"
      /mol_type="unassigned DNA"

<400> 42
tcttcgggaa ctagataggt ggc                                            23


<210> 43
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..23
<223> /organism="Artificial Sequence"
      /note="primer 290-Hx2inr"
      /mol_type="unassigned DNA"

<400> 43
gaagtaatca gccacaatac gcc                                            23


<210> 44
<211> 73
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..73
<223> /organism="Artificial Sequence"
      /note="primer 838-Glk1-psuc227f"
      /mol_type="unassigned DNA"

<400> 44
atgtcattcg acgacttaca caaagccact gagagagcgg tcatccaggc ccgtcgacct     60

cgagtaccgt tcg                                                        73


<210> 45
<211> 73
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..73
<223> /organism="Artificial Sequence"
      /note="primer 834- Hxk2-psuc227f"
      /mol_type="unassigned DNA"

<400> 45
gccagaaagg gttccatggc cgatgtgcca aaggaattga tgcaacaaat ccgtcgacct     60

cgagtaccgt tcg                                                        73


<210> 46
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /organism="Artificial Sequence"
      /note="primer 645-pSUC227r"
      /mol_type="unassigned DNA"

<400> 46
gcaatttcgg ctatacgtaa c                                              21


<210> 47
<211> 73
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..73
<223> /organism="Artificial Sequence"
      /note="primer 839-Glk1-psuc225r"
      /mol_type="unassigned DNA"

<400> 47
caatcttcaa gtgcaccttc ctctcaccct cggcacccaa gggtgacaag ccggatccta     60

ccgttcgtat agc                                                        73


<210> 48
<211> 73
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..73
<223> /organism="Artificial Sequence"
      /note="primer 835-Hxk2-psuc225r"
      /mol_type="unassigned DNA"

<400> 48
gccagaaagg gttccatggc cgatgtgcca aaggaattga tgcaacaaat ccgtcgacct     60

cgagtaccgt tcg                                                        73


<210> 49
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /organism="Artificial Sequence"
      /note="primer 646-pSUC225f"
      /mol_type="unassigned DNA"

<400> 49
cgttcactca tggaaaatag c                                              21


<210> 50
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..72
<223> /organism="Artificial Sequence"
      /note="primer 846-Hxk1_loxP_f"
      /mol_type="unassigned DNA"

<400> 50
atggttcatt taggtccaaa gaaaccacag gctagaaagg gttccatggc cggatccact     60

agcataactt cg                                                         72


<210> 51
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..72
<223> /organism="Artificial Sequence"
      /note="primer 847-Hxk1_loxP_r"
      /mol_type="unassigned DNA"

<400> 51
atggttcatt taggtccaaa gaaaccacag gctagaaagg gttccatggc cggatccact     60

agcataactt cg                                                         72


<210> 52
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..72
<223> /organism="Artificial Sequence"
      /note="primer 848-Gal1_loxP_f"
      /mol_type="unassigned DNA"

<400> 52
atgactaaat ctcattcaga agaagtgatt gtacctgagt tcaattctag cggatccact     60

agcataactt cg                                                         72


<210> 53
<211> 70
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..70
<223> /organism="Artificial Sequence"
      /note="primer 849-Gal1_loxP_r"
      /mol_type="unassigned DNA"

<400> 53
ttataattca tatagacagc tgcccaatgc tggtttagag acgatgatag ttgggccgcc     60

agtgtgatgg                                                            70


<210> 54
<211> 5250
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..5250
<223> /organism="Artificial Sequence"
      /note="pRN774; TOPO-BLUNT-loxP-hphMX-loxP (loxP sites in opposite
      orientation) "
      /mol_type="unassigned DNA"

<400> 54
gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag       60

ctatgaccat gattacgcca agctatttag gtgacactat agaatactca agctatgcat      120

caagcttggt accgagctcg gatccactag cataacttcg tataatgtat gctatacgaa      180

gttattctag taacggccgc cagtgtgctg gaattcgccc ttaagctttg cctcgtcccc      240

gccgggtcac ccggccagcg acatggaggc ccagaatacc ctccttgaca gtcttgacgt      300

gcgcagctca ggggcatgat gtgactgtcg cccgtacatt tagcccatac atccccatgt      360

ataatcattt gcatccatac attttgatgg ccgcacggcg cgaagcaaaa attacggctc      420

ctcgctgcag acctgcgagc agggaaacgc tcccctcaca gacgcgtgaa ttgtccccac      480

gccgcgcccc tgtagagaaa tataaaaggt taggatttgc cactgaggtt cttctttcat      540

atacttcctt ttaaaatctt gctaggatac agttctcaca tcacatccga acataaacaa      600

ccatgggtaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt      660

tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct      720

tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca      780

aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg      840

acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca      900

cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca      960

tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc     1020

aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg     1080

tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg     1140

atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt     1200

tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg     1260

aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt     1320

tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat     1380

cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg     1440

ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat     1500

ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg     1560

atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg     1620

caaaggaata atcagtactg acaataaaaa gattcttgtt ttcaagaact tgtcatttgt     1680

atagtttttt tatattgtag ttgttctatt ttaatcaaat gttagcgtga tttatatttt     1740

ttttcgcctc gacatcatct gcccagatgc gaagttaagt gcgcagaaag taatatcatg     1800

cgtcaatcgt atgtgaatgc tggtcgctat actgctgtcg attcgatact aacgccgcca     1860

tccagtgtcg aaaacgagct cgaattcatc gatgatatcc atcacactgg cggccgctcg     1920

acgatatcta gagcgcgcat aacttcgtat aatgtatgct atacgaagtt ataggatcca     1980

tcacactggc ggcccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt     2040

ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat     2100

ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag     2160

ttgcgcagcc tatacgtacg gcagtttaag gtttacacct ataaaagaga gagccgttat     2220

cgtctgtttg tggatgtaca gagtgatatt attgacacgc cggggcgacg gatggtgatc     2280

cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg     2340

catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc     2400

gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt     2460

aacctgatgt tctggggaat ataaatgtca ggcatgagat tatcaaaaag gatcttcacc     2520

tagatccttt tcacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc     2580

agctactggg ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc     2640

agtgggctta catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa     2700

ttgccagctg gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct     2760

ttctcgccgc caaggatctg atggcgcagg ggatcaagct ctgatcaaga gacaggatga     2820

ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg     2880

gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg     2940

ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc     3000

ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct     3060

tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa     3120

gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg     3180

gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa     3240

gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat     3300

gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg     3360

agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc     3420

atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac     3480

cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg     3540

gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc     3600

tatcgccttc ttgacgagtt cttctgaatt attaacgctt acaatttcct gatgcggtat     3660

tttctcctta cgcatctgtg cggtatttca caccgcatac aggtggcact tttcggggaa     3720

atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca     3780

tgagacaata accctgataa atgcttcaat aatagcacgt gaggagggcc accatggcca     3840

agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct     3900

ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc     3960

gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc     4020

tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt     4080

ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg     4140

ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc     4200

aggactgaca cgtgctaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt     4260

ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc     4320

ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct     4380

tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa     4440

ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag     4500

tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc     4560

tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg     4620

actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca     4680

cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat     4740

gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg     4800

tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc     4860

ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc     4920

ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctgggc ttttgctggc     4980

cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg     5040

cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcaagcg     5100

cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga     5160

caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac     5220

tcattaggca ccccaggctt tacactttat                                      5250


<210> 55
<211> 4744
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..4744
<223> /organism="Artificial Sequence"
      /note="pRN775; TOPO-BLUNT-loxP-natMX-loxP (loxP sites in opposite
      orientation)"
      /mol_type="unassigned DNA"

<400> 55
gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag       60

ctatgaccat gattacgcca agctatttag gtgacactat agaatactca agctatgcat      120

caagcttggt accgagctcg gatccactag cataacttcg tataatgtat gctatacgaa      180

gttattctag taacggccgc cagtgtgctg gaattcgccc ttaagcttgc ctcgtccccg      240

ccgggtcacc cggccagcga catggaggcc cagaataccc tccttgacag tcttgacgtg      300

cgcagctcag gggcatgatg tgactgtcgc ccgtacattt agcccataca tccccatgta      360

taatcatttg catccataca ttttgatggc cgcacggcgc gaagcaaaaa ttacggctcc      420

tcgctgcaga cctgcgagca gggaaacgct cccctcacag acgcgttgaa ttgtccccac      480

gccgcgcccc tgtagagaaa tataaaaggt taggatttgc cactgaggtt cttctttcat      540

atacttcctt ttaaaatctt gctaggatac agttctcaca tcacatccga acataaacaa      600

ccatgtaaaa tgaccactct tgacgacacg gcttaccggt accgcaccag tgtcccgggg      660

gacgccgagg ccatcgaggc actggatggg tccttcacca ccgacaccgt cttccgcgtc      720

accgccaccg gggacggctt caccctgcgg gaggtgccgg tggacccgcc cctgaccaag      780

gtgttccccg acgacgaatc ggacgacgaa tcggacgccg gggaggacgg cgacccggac      840

tcccggacgt tcgtcgcgta cggggacgac ggcgacctgg cgggcttcgt ggtcgtctcg      900

tactccggct ggaaccgccg gctgaccgtc gaggacatcg aggtcgcccc ggagcaccgg      960

gggcacgggg tcgggcgcgc gttgatgggg ctcgcgacgg agttcgcccg cgagcggggc     1020

gccgggcacc tctggctgga ggtcaccaac gtcaacgcac cggcgatcca cgcgtaccgg     1080

cggatggggt tcaccctctg cggcctggac accgccctgt acgacggcac cgcctcggac     1140

ggcgagcagg cgctctacat gagcatgccc tgcccctagt actgacaata aaaagattct     1200

tgttttcaag aacttgtcat ttgtatagtt tttttatatt gtagttgttc tattttaatc     1260

aaatgttagc gtgatttata ttttttttcg cctcgacatc atctgcccag atgcgaagtt     1320

aagtgcgcag aaagtaatat catgcgtcaa tcgtatgtga atgctggtcg ctatactgct     1380

gtcgattcga tactaacgcc gccatccagt gtcgacgata tctagagcgc gcataacttc     1440

gtataatgta tgctatacga agttatagga tccatcacac tggcggccca attcgcccta     1500

tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc     1560

tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag     1620

cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctatacg tacggcagtt     1680

taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga     1740

tattattgac acgccggggc gacggatggt gatccccctg gccagtgcac gtctgctgtc     1800

agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat     1860

gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct     1920

cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat     1980

gtcaggcatg agattatcaa aaaggatctt cacctagatc cttttcacgt agaaagccag     2040

tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga     2100

aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga     2160

ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa     2220

ggttgggaag ccctgcaaag taaactggat ggctttctcg ccgccaagga tctgatggcg     2280

caggggatca agctctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga     2340

tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc     2400

acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc     2460

ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc     2520

gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac     2580

tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc     2640

tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac     2700

gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg     2760

tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct     2820

cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt     2880

cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg     2940

attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac     3000

ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg     3060

tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg     3120

aattattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat     3180

ttcacaccgc atacaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta     3240

tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt     3300

caataatagc acgtgaggag ggccaccatg gccaagttga ccagtgccgt tccggtgctc     3360

accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg     3420

gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc     3480

gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg     3540

gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg     3600

ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg     3660

gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct aaaacttcat     3720

ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct     3780

taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct     3840

tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca     3900

gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc     3960

agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc     4020

aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct     4080

gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag     4140

gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc     4200

tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg     4260

agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag     4320

cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt     4380

gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac     4440

gcggcctttt tacggttcct gggcttttgc tggccttttg ctcacatgtt ctttcctgcg     4500

ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc     4560

cgcagccgaa cgaccgagcg cagcgagtca agcgcccaat acgcaaaccg cctctccccg     4620

cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca     4680

gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact     4740

ttat                                                                  4744


<210> 56
<211> 1725
<212> DNA
<213> Saccharomyces cerevisiae

<220> 
<221> source
<222> 1..1725
<223> /organism="Saccharomyces cerevisiae"
      /note="WT-GAL2 DNA sequence "
      /mol_type="unassigned DNA"

<400> 56
atggcagttg aggagaacaa tatgcctgtt gtttcacagc aaccccaagc tggtgaagac      60

gtgatctctt cactcagtaa agattcccat ttaagcgcac aatctcaaaa gtattccaat     120

gatgaattga aagccggtga gtcagggcct gaaggctccc aaagtgttcc tatagagata     180

cccaagaagc ccatgtctga atatgttacc gtttccttgc tttgtttgtg tgttgccttc     240

ggcggcttca tgtttggctg ggataccggt actatttctg ggtttgttgt ccaaacagac     300

tttttgagaa ggtttggtat gaaacataag gatggtaccc actatttgtc aaacgtcaga     360

acaggtttaa tcgtcgccat tttcaatatt ggctgtgcct ttggtggtat tatactttcc     420

aaaggtggag atatgtatgg ccgtaaaaag ggtctttcga ttgtcgtctc ggtttatata     480

gttggtatta tcattcaaat tgcctctatc aacaagtggt accaatattt cattggtaga     540

atcatatctg gtttgggtgt cggcggcatc gccgtcttat gtcctatgtt gatctctgaa     600

attgctccaa agcacttgag aggcacacta gtttcttgtt atcagctgat gattactgca     660

ggtatctttt tgggctactg tactaattac ggtacaaaga gctattcgaa ctcagttcaa     720

tggagagttc cattagggct atgtttcgct tggtcattat ttatgattgg cgctttgacg     780

ttagttcctg aatccccacg ttatttatgt gaggtgaata aggtagaaga cgccaagctt     840

tccattgcta agtctaacaa ggtgtcacca gaggatcctg ccgtccaggc agagttagat     900

ctgatcatgg ccggtataga agctgaaaaa ctggctggca atgcgtcctg gggggaatta     960

ttttccacca agaccaaagt atttcaacgt ttgttgatgg gtgtatttgt tcaaatgttc    1020

caacaattaa ccggtaacaa ttattttttc tactacggta ccgttatttt caagtcagtt    1080

ggcctggatg attcctttga aacatccatt gtcattggtg tagtcaactt tgcctccact    1140

ttctttagtt tgtggactgt cgaaaacttg gggcgtcgta aatgtttact tttgggcgct    1200

gccactatga tggcttgtat ggtcatctac gcctctgttg gtgttaccag attatatcct    1260

cacggtaaaa gccagccatc ttctaaaggt gccggtaact gtatgattgt ctttacctgt    1320

ttttatattt tctgttatgc cacaacctgg gcgccagttg cctgggtcat cacagcagaa    1380

tcattcccac tgagagtcaa gtcgaaatgt atggcgttgg cctctgcttc caattgggta    1440

tgggggttct tgattgcatt tttcacccca ttcatcacat ctgccattaa cttctactac    1500

ggttatgtct tcatgggctg tttggttgcc atgttttttt atgtcttttt ctttgttcca    1560

gaaactaaag gcctatcgtt agaagaaatt caagaattat gggaagaagg tgttttacct    1620

tggaaatctg aaggctggat tccttcatcc agaagaggta ataattacga tttagaggat    1680

ttacaacatg acgacaaacc gtggtacaag gccatgctag aataa                    1725


<210> 57
<211> 574
<212> PRT
<213> Saccharomyces cerevisiae

<220> 
<223> WT Gal2p amino acid sequence

<400> 57
Met Ala Val Glu Glu Asn Asn Met Pro Val Val Ser Gln Gln Pro Gln 
1               5                   10                  15      
Ala Gly Glu Asp Val Ile Ser Ser Leu Ser Lys Asp Ser His Leu Ser 
            20                  25                  30          
Ala Gln Ser Gln Lys Tyr Ser Asn Asp Glu Leu Lys Ala Gly Glu Ser 
        35                  40                  45              
Gly Pro Glu Gly Ser Gln Ser Val Pro Ile Glu Ile Pro Lys Lys Pro 
    50                  55                  60                  
Met Ser Glu Tyr Val Thr Val Ser Leu Leu Cys Leu Cys Val Ala Phe 
65                  70                  75                  80  
Gly Gly Phe Met Phe Gly Trp Asp Thr Gly Thr Ile Ser Gly Phe Val 
                85                  90                  95      
Val Gln Thr Asp Phe Leu Arg Arg Phe Gly Met Lys His Lys Asp Gly 
            100                 105                 110         
Thr His Tyr Leu Ser Asn Val Arg Thr Gly Leu Ile Val Ala Ile Phe 
        115                 120                 125             
Asn Ile Gly Cys Ala Phe Gly Gly Ile Ile Leu Ser Lys Gly Gly Asp 
    130                 135                 140                 
Met Tyr Gly Arg Lys Lys Gly Leu Ser Ile Val Val Ser Val Tyr Ile 
145                 150                 155                 160 
Val Gly Ile Ile Ile Gln Ile Ala Ser Ile Asn Lys Trp Tyr Gln Tyr 
                165                 170                 175     
Phe Ile Gly Arg Ile Ile Ser Gly Leu Gly Val Gly Gly Ile Ala Val 
            180                 185                 190         
Leu Cys Pro Met Leu Ile Ser Glu Ile Ala Pro Lys His Leu Arg Gly 
        195                 200                 205             
Thr Leu Val Ser Cys Tyr Gln Leu Met Ile Thr Ala Gly Ile Phe Leu 
    210                 215                 220                 
Gly Tyr Cys Thr Asn Tyr Gly Thr Lys Ser Tyr Ser Asn Ser Val Gln 
225                 230                 235                 240 
Trp Arg Val Pro Leu Gly Leu Cys Phe Ala Trp Ser Leu Phe Met Ile 
                245                 250                 255     
Gly Ala Leu Thr Leu Val Pro Glu Ser Pro Arg Tyr Leu Cys Glu Val 
            260                 265                 270         
Asn Lys Val Glu Asp Ala Lys Leu Ser Ile Ala Lys Ser Asn Lys Val 
        275                 280                 285             
Ser Pro Glu Asp Pro Ala Val Gln Ala Glu Leu Asp Leu Ile Met Ala 
    290                 295                 300                 
Gly Ile Glu Ala Glu Lys Leu Ala Gly Asn Ala Ser Trp Gly Glu Leu 
305                 310                 315                 320 
Phe Ser Thr Lys Thr Lys Val Phe Gln Arg Leu Leu Met Gly Val Phe 
                325                 330                 335     
Val Gln Met Phe Gln Gln Leu Thr Gly Asn Asn Tyr Phe Phe Tyr Tyr 
            340                 345                 350         
Gly Thr Val Ile Phe Lys Ser Val Gly Leu Asp Asp Ser Phe Glu Thr 
        355                 360                 365             
Ser Ile Val Ile Gly Val Val Asn Phe Ala Ser Thr Phe Phe Ser Leu 
    370                 375                 380                 
Trp Thr Val Glu Asn Leu Gly Arg Arg Lys Cys Leu Leu Leu Gly Ala 
385                 390                 395                 400 
Ala Thr Met Met Ala Cys Met Val Ile Tyr Ala Ser Val Gly Val Thr 
                405                 410                 415     
Arg Leu Tyr Pro His Gly Lys Ser Gln Pro Ser Ser Lys Gly Ala Gly 
            420                 425                 430         
Asn Cys Met Ile Val Phe Thr Cys Phe Tyr Ile Phe Cys Tyr Ala Thr 
        435                 440                 445             
Thr Trp Ala Pro Val Ala Trp Val Ile Thr Ala Glu Ser Phe Pro Leu 
    450                 455                 460                 
Arg Val Lys Ser Lys Cys Met Ala Leu Ala Ser Ala Ser Asn Trp Val 
465                 470                 475                 480 
Trp Gly Phe Leu Ile Ala Phe Phe Thr Pro Phe Ile Thr Ser Ala Ile 
                485                 490                 495     
Asn Phe Tyr Tyr Gly Tyr Val Phe Met Gly Cys Leu Val Ala Met Phe 
            500                 505                 510         
Phe Tyr Val Phe Phe Phe Val Pro Glu Thr Lys Gly Leu Ser Leu Glu 
        515                 520                 525             
Glu Ile Gln Glu Leu Trp Glu Glu Gly Val Leu Pro Trp Lys Ser Glu 
    530                 535                 540                 
Gly Trp Ile Pro Ser Ser Arg Arg Gly Asn Asn Tyr Asp Leu Glu Asp 
545                 550                 555                 560 
Leu Gln His Asp Asp Lys Pro Trp Tyr Lys Ala Met Leu Glu 
                565                 570                 

<210> 58
<211> 8052
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..8052
<223> /organism="Artificial Sequence"
      /note="pRS313-GAL2 (GAL2 expression vector uSEQ as host vector
      for error prone mutagenesis library"
      /mol_type="unassigned DNA"

<400> 58
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagkc agacaagccc gtcagggcgc gtcagcgggt      120

gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg      180

caccataatt ccgttttaag agcttggtga gcgctaggag tcactgccag gtatcgtttg      240

aacacggcat tagtcaggga agtcataaca cagtcctttc ccgcaatttt ctttttctat      300

tactcttggc ctcctctagt acactctata tttttttatg cctcggtaat gattttcatt      360

tttttttttc cacctagcgg atgactcttt ttttttctta gcgattggca ttatcacata      420

atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc      480

aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa      540

atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact      600

cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga      660

ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt      720

ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca      780

ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctactg gcgcgtggag      840

taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag      900

atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag      960

atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta     1020

ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca     1080

aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct     1140

ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat     1200

atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat     1260

actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt     1320

ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc atatgcggtg     1380

tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt aaacgttaat     1440

attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc     1500

gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt     1560

ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa     1620

accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg     1680

tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga     1740

cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct     1800

agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat     1860

gcgccgctac agggcgcgtc gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg     1920

cgatcggtgc gggcctcttc gctattacgc cagctggcga aggggggatg tgctgcaagg     1980

cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt     2040

gaattgtaat acgactcact atagggcgaa ttggagctct ctcgtaggaa caatttcggg     2100

cccctgcgtg ttcttctgag gttcatcttt tacatttgct tctgctggat aattttcaga     2160

ggcaacaagg aaaaattaga tggcaaaaag tcgtctttca aggaaaaatc cccaccatct     2220

ttcgagatcc cctgtaactt attggcaact gaaagaatga aaaggaggaa aatacaaaat     2280

atactagaac tgaaaaaaaa aaagtataaa tagagacgat atatgccaat acttcacaat     2340

gttcgaatct attcttcatt tgcagctatt gtaaaataat aaaacatcaa gaacaaacaa     2400

gctcaacttg tcttttctaa gaacaaagaa taaacacaaa aacaaaaagt ttttttaatt     2460

ttaatcaaaa atctagaatg gcagttgagg agaacaatat gcctgttgtt tcacagcaac     2520

cccaagctgg tgaagacgtg atctcttcac tcagtaaaga ttcccattta agcgcacaat     2580

ctcaaaagta ttccaatgat gaattgaaag ccggtgagtc agggcctgaa ggctcccaaa     2640

gtgttcctat agagataccc aagaagccca tgtctgaata tgttaccgtt tccttgcttt     2700

gtttgtgtgt tgccttcggc ggcttcatgt ttggctggga taccggtact atttctgggt     2760

ttgttgtcca aacagacttt ttgagaaggt ttggtatgaa acataaggat ggtacccact     2820

atttgtcaaa cgtcagaaca ggtttaatcg tcgccatttt caatattggc tgtgcctttg     2880

gtggtattat actttccaaa ggtggagata tgtatggccg taaaaagggt ctttcgattg     2940

tcgtctcggt ttatatagtt ggtattatca ttcaaattgc ctctatcaac aagtggtacc     3000

aatatttcat tggtagaatc atatctggtt tgggtgtcgg cggcatcgcc gtcttatgtc     3060

ctatgttgat ctctgaaatt gctccaaagc acttgagagg cacactagtt tcttgttatc     3120

agctgatgat tactgcaggt atctttttgg gctactgtac taattacggt acaaagagct     3180

attcgaactc agttcaatgg agagttccat tagggctatg tttcgcttgg tcattattta     3240

tgattggcgc tttgacgtta gttcctgaat ccccacgtta tttatgtgag gtgaataagg     3300

tagaagacgc caagctttcc attgctaagt ctaacaaggt gtcaccagag gatcctgccg     3360

tccaggcaga gttagatctg atcatggccg gtatagaagc tgaaaaactg gctggcaatg     3420

cgtcctgggg ggaattattt tccaccaaga ccaaagtatt tcaacgtttg ttgatgggtg     3480

tatttgttca aatgttccaa caattaaccg gtaacaatta ttttttctac tacggtaccg     3540

ttattttcaa gtcagttggc ctggatgatt cctttgaaac atccattgtc attggtgtag     3600

tcaactttgc ctccactttc tttagtttgt ggactgtcga aaacttgggg cgtcgtaaat     3660

gtttactttt gggcgctgcc actatgatgg cttgtatggt catctacgcc tctgttggtg     3720

ttaccagatt atatcctcac ggtaaaagcc agccatcttc taaaggtgcc ggtaactgta     3780

tgattgtctt tacctgtttt tatattttct gttatgccac aacctgggcg ccagttgcct     3840

gggtcatcac agcagaatca ttcccactga gagtcaagtc gaaatgtatg gcgttggcct     3900

ctgcttccaa ttgggtatgg gggttcttga ttgcattttt caccccattc atcacatctg     3960

ccattaactt ctactacggt tatgtcttca tgggctgttt ggttgccatg tttttttatg     4020

tctttttctt tgttccagaa actaaaggcc tatcgttaga agaaattcaa gaattatggg     4080

aagaaggtgt tttaccttgg aaatctgaag gctggattcc ttcatccaga agaggtaata     4140

attacgattt agaggattta caacatgacg acaaaccgtg gtacaaggcc atgctagaat     4200

aacccgggct gcaggaattc gatatcaagc ttatcgattt tgcgaacact tttattaatt     4260

catgatcacg ctctaatttg tgcatttgaa atgtactcta attctaattt tatattttta     4320

atgatatctt gaaaagtaaa tacgttttta atatatacaa aataatacag tttaattttc     4380

aagtttttga tcatttgttc tcagaaagtt gagtgggacg gagacaaaga aactttaaag     4440

agaaatgcaa agtgggaaga agtcagttgt ttaccgaccg cactgttatt cacaaatatt     4500

ccaattttgc ctgcagaccc acgtctacaa attttggtta gtttggtaaa tggtaaggat     4560

atagtagagc ctttttgaaa tgggaaatat cttctttttc tgtatcccgc ttcaaaaagt     4620

gtctaatgag tcagttattt ctttcttact catcgcccgt cacttaaaag aagaaaaatt     4680

actttcatga tgcgaagcga aaaaaatttt tagcttcaat tttcacaatg catctatgga     4740

gaggatatta taaggttacg aaataaattc ttgagtgttg taaattctgt taatcaaaga     4800

aaaagcaata gctcgttttt ctacagaatg gctagcacag caaatatgat ttctcaacta     4860

aagaaactat ctatcgcgga acctgcggtt gccaaggatt ctcatcctga tgtaaatatc     4920

gtggatttga tgagaaatta catttcacaa gaattaagca agatttccgg cgtagactcg     4980

tctcttattt ttccagcatt agaatggaca aatactatgg agagaggcga tttgttgatt     5040

cctatcccaa gattgagaat caaaggtgcc aatccaaagg atttggcagt ccaatgggct     5100

gaaaaatttc catgtggaga tttcttggag aaagttgaag cgaatggccc gttcatccag     5160

tttttcttca accctcaatt tttagcaaaa cttgtcatcc cagatatctt aaccagaaag     5220

gaggattatg gttcttgcgt cgacctcgag ggggggcccg gtacccagct tttgttccct     5280

ttagtgaggg ttaattccga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa     5340

ttgttatccg ctcacaattc cacacaacat aggagccgga agcataaagt gtaaagcctg     5400

gggtgcctaa tgagtgaggt aactcacatt aattgcgttg cgctcactgc ccgctttcca     5460

gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg     5520

tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg     5580

gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg     5640

ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa     5700

ggccgcgttg ctggcgtttt tccataggct cggcccccct gacgagcatc acaaaaatcg     5760

acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgttcccccc     5820

tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc     5880

ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc     5940

ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg     6000

ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc     6060

actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga     6120

gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc     6180

tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac     6240

caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg     6300

atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc     6360

acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa     6420

ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta     6480

ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt     6540

tgcctgactg cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag     6600

tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca     6660

gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc     6720

tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt     6780

tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag     6840

ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgaa aaaaagcggt     6900

tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat     6960

ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt     7020

gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc     7080

ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat     7140

cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag     7200

ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt     7260

ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg     7320

gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta     7380

ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc     7440

gcgcacattt ccccgaaaag tgccacctgg gtccttttca tcacgtgcta taaaaataat     7500

tataatttaa attttttaat ataaatatat aaattaaaaa tagaaagtaa aaaaagaaat     7560

taaagaaaaa atagtttttg ttttccgaag atgtaaaaga ctctaggggg atcgccaaca     7620

aatactacct tttatcttgc tcttcctgct ctcaggtatt aatgccgaat tgtttcatct     7680

tgtctgtgta gaagaccaca cacgaaaatc ctgtgatttt acattttact tatcgttaat     7740

cgaatgtata tctatttaat ctgcttttct tgtctaataa atatatatgt aaagtacgct     7800

ttttgttgaa attttttaaa cctttgttta tttttttttc ttcattccgt aactcttcta     7860

ccttctttat ttactttcta aaatccaaat acaaaacata aaaataaata aacacagagt     7920

aaattcccaa attattccat cattaaaaga tacgaggcgc gtgtaagtta caggcaagcg     7980

atccgtccta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga     8040

ggccctttcg tc                                                         8052


<210> 59
<211> 9402
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..9402
<223> /organism="Artificial Sequence"
      /note="pRN187 (pSH65-derived CRE recombinase expressing vector)"
      /mol_type="unassigned DNA"

<400> 59
cctctagagt cgacctgcag cttctcaatg atattcgaat acgctttgag gagatacagc       60

ctaatatccg acaaactgtt ttacagattt acgatcgtac ttgttaccca tcattgaatt      120

ttgaacatcc gaacctggga gttttccctg aaacagatag tatatttgaa cctgtataat      180

aatatatagt ctagcgcttt acggaagaca atgtatgtat ttcggttcct ggagaaacta      240

ttgcatctat tgcataggta atcttgcacg tcgcatcccc ggttcatttt ctgcgtttcc      300

atcttgcact tcaatagcat atctttgtta acgaagcatc tgtgcttcat tttgtagaac      360

aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag      420

aacagaaatg caacgcgaaa gcgctatttt accaacgaag aatctgtgct tcatttttgt      480

aaaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt      540

tacagaacag aaatgcaacg cgagagcgct attttaccaa caaagaatct atacttcttt      600

tttgttctac aaaaatgcat cccgagagcg ctatttttct aacaaagcat cttagattac      660

tttttttctc ctttgtgcgc tctataatgc agtctcttga taactttttg cactgtaggt      720

ccgttaaggt tagaagaagg ctactttggt gtctattttc tcttccataa aaaaagcctg      780

actccacttc ccgcgtttac tgattactag cgaagctgcg ggtgcatttt ttcaagataa      840

aggcatcccc gattatattc tataccgatg tggattgcgc atactttgtg aacagaaagt      900

gatagcgttg atgattcttc attggtcaga aaattatgaa cggtttcttc tattttgtct      960

ctatatacta cgtataggaa atgtttacat tttcgtattg ttttcgattc actctatgaa     1020

tagttcttac tacaattttt ttgtctaaag agtaatacta gagataaaca taaaaaatgt     1080

agaggtcgag tttagatgca agttcaagga gcgaaaggtg gatgggtagg ttatataggg     1140

atatagcaca gagatatata gcaaagagat acttttgagc aatgtttgtg gaagcggtat     1200

tcgcaatatt ttagtagctc gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc     1260

ttcagagcgc ttttggtttt caaaagcgct ctgaagttcc tatactttct agagaatagg     1320

aacttcggaa taggaacttc aaagcgtttc cgaaaacgag cgcttccgaa aatgcaacgc     1380

gagctgcgca catacagctc actgttcacg tcgcacctat atctgcgtgt tgcctgtata     1440

tatatataca tgagaagaac ggcatagtgc gtgtttatgc ttaaatgcgt acttatatgc     1500

gtctatttat gtaggatgaa aggtagtcta gtacctcctg tgatattatc ccattccatg     1560

cggggtatcg tatgcttcct tcagcactac cctttagctg ttctatatgc tgccactcct     1620

caattggatt agtctcatcc ttcaatgcta tcatttcctt tgatattgga tcatatgcat     1680

agtaccgaga aactagtgcg aagtagtgat caggtattgc tgttatctga tgagtatacg     1740

ttgtcctggc cacggcagaa gcacgcttat cgctccaatt tcccacaaca ttagtcaact     1800

ccgttaggcc cttcattgaa agaaatgagg tcatcaaatg tcttccaatg tgagattttg     1860

ggccattttt tatagcaaag attgaataag gcgcattttt cttcaaagct ttattgtacg     1920

atctgactaa gttatctttt aataattggt attcctgttt attgcttgaa gaattgccgg     1980

tcctatttac tcgttttagg actggttcag aattcttgaa gacgaaaggg cctcgtgata     2040

cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact     2100

tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg     2160

tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt     2220

atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct     2280

gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca     2340

cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc     2400

gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc     2460

cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg     2520

gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta     2580

tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc     2640

ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt     2700

gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg     2760

cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct     2820

tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc     2880

tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct     2940

cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac     3000

acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc     3060

tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat     3120

ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg     3180

accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc     3240

aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa     3300

ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag     3360

gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta     3420

ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta     3480

ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag     3540

ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg     3600

gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg     3660

cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag     3720

cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc     3780

cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa     3840

aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg     3900

ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct     3960

gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa     4020

gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg     4080

cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttac     4140

ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcctat gttgtgtgga     4200

attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc     4260

gcaattaacc ctcactaaag ggaacaaaag ctggagctct agtacggatt agaagccgcc     4320

gagcgggtga cagccctccg aaggaagact ctcctccgtg cgtcctcgtc ttcaccggtc     4380

gcgttcctga aacgcagatg tgcctcgcgc cgcactgctc cgaacaataa agattctaca     4440

atactagctt ttatggttat gaagaggaaa aattggcagt aacctggccc cacaaacctt     4500

caaatgaacg aatcaaatta acaaccatag gatgataatg cgattagttt tttagcctta     4560

tttctggggt aattaatcag cgaagcgatg atttttgatc tattaacaga tatataaatg     4620

caaaaactgc ataaccactt taactaatac tttcaacatt ttcggtttgt attacttctt     4680

attcaaatgt aataaaagta tcaacaaaaa attgttaata tacctctata ctttaacgtc     4740

aaggagaaaa aaccccggat tctagaacta gtggatcccc cgggctgcag gaattcgata     4800

tcaagcttat cgataccgtc gaggggcaga gccgatcctg tacactttac ttaaaaccat     4860

tatctgagtg ttaaatgtcc aatttactga ccgtacacca aaatttgcct gcattaccgg     4920

tcgatgcaac gagtgatgag gttcgcaaga acctgatgga catgttcagg gatcgccagg     4980

cgttttctga gcatacctgg aaaatgcttc tgtccgtttg ccggtcgtgg gcggcatggt     5040

gcaagttgaa taaccggaaa tggtttcccg cagaacctga agatgttcgc gattatcttc     5100

tatatcttca ggcgcgcggt ctggcagtaa aaactatcca gcaacatttg ggccagctaa     5160

acatgcttca tcgtcggtcc gggctgccac gaccaagtga cagcaatgct gtttcactgg     5220

ttatgcggcg gatccgaaaa gaaaacgttg atgccggtga acgtgcaaaa caggctctag     5280

cgttcgaacg cactgatttc gaccaggttc gttcactcat ggaaaatagc gatcgctgcc     5340

aggatatacg taatctggca tttctgggga ttgcttataa caccctgtta cgtatagccg     5400

aaattgccag gatcagggtt aaagatatct cacgtactga cggtgggaga atgttaatcc     5460

atattggcag aacgaaaacg ctggttagca ccgcaggtgt agagaaggca cttagcctgg     5520

gggtaactaa actggtcgag cgatggattt ccgtctctgg tgtagctgat gatccgaata     5580

actacctgtt ttgccgggtc agaaaaaatg gtgttgccgc gccatctgcc accagccagc     5640

tatcaactcg cgccctggaa gggatttttg aagcaactca tcgattgatt tacggcgcta     5700

aggatgactc tggtcagaga tacctggcct ggtctggaca cagtgcccgt gtcggagccg     5760

cgcgagatat ggcccgcgct ggagtttcaa taccggagat catgcaagct ggtggctgga     5820

ccaatgtaaa tattgtcatg aactatatcc gtaccctgga tagtgaaaca ggggcaatgg     5880

tgcgcctgct ggaagatggc gattagccat taacgcgtaa atgattgcta taattatttg     5940

atatttatgg tgacatatga gaaaggattt caacatcgac ggaaaatatg tagtgctgtc     6000

tgtaagcact aatattcagt cgccagccgt cattgtcact gtaaagctga gcgatagaat     6060

gcctgatatt gactcaatat ccgttgcgtt tcctgtcaaa agtatgcgta gtgctgaaca     6120

tttcgtgatg aatgccaccg aggaagaagc acggcgcggt tttgcntaaa gtgatgtctg     6180

agtttggcga actcttgggt aaggttggaa ttgtcgacct cgagtcatgt aattagttat     6240

gtcacgctta cattcacgcc ctccccccac atccgctcta accgaaaagg aaggagttag     6300

acaacctgaa gtctaggtcc ctatttattt ttttatagtt atgttagtat taagaacgtt     6360

atttatattt caaatttttc ttttttttct gtacagacgc gtgtacgcat gtaacattat     6420

actgaaaacc ttgcttgaga aggttttggg acgctcgaag gctttaattt gcggccggta     6480

cctaggaccg gtttatcatt atcaatactg ccatttcaaa gaatacgtaa ataattaata     6540

gtagtgattt tcctaacttt atttagtcaa aaaattagcc ttttaattct gctgtaaccc     6600

gtacatgccc aaaatagggg gcgggttaca cagaatatat aacatcgtag gtgtctgggt     6660

gaacagttta ttcctggcat ccactaaata taatggagcc cgctttttaa gctggcatcc     6720

agaaaaaaaa agaatcccag caccaaaata ttgttttctt caccaaccat cagttcatag     6780

gtccattctc ttagcgcaac tacagagaac aggggcacaa acaggcaaaa aacgggcaca     6840

acctcaatgg agtgatgcaa cctgcctgga gtaaatgatg acacaaggca attgacccac     6900

gcatgtatct atctcatttt cttacacctt ctattacctt ctgctctctc tgatttggaa     6960

aaagctgaaa aaaaaggttg aaaccagttc cctgaaatta ttcccctact tgactaataa     7020

gtatataaag acggtaggta ttgattgtaa ttctgtaaat ctatttctta aacttcttaa     7080

attctacttt tatagttagt ctttttttta gttttaaaac accaagaact tagtttcgaa     7140

taaacacaca taaagaattc aaaatggttt caaaaggtga agaagataat atggctatta     7200

ttaaagaatt tatgagattt aaagttcata tggaaggttc agttaatggt catgaatttg     7260

aaattgaagg tgaaggtgaa ggtagaccat atgaaggtac tcaaactgct aaattgaaag     7320

ttactaaagg tggtccatta ccatttgctt gggatatttt gtcaccacaa tttatgtatg     7380

gttcaaaagc ttatgttaaa catccagctg atattccaga ttatttaaaa ttgtcatttc     7440

cagaaggttt taaatgggaa agagttatga attttgaaga tggtggtgtt gttactgtta     7500

ctcaagattc atcattacaa gatggtgaat ttatttataa agttaaattg agaggtacta     7560

attttccatc agatggtcca gttatgcaaa aaaaaactat gggttgggaa gcttcatcag     7620

aaagaatgta tccagaagat ggtgctttaa aaggtgaaat taaacaaaga ttgaaattaa     7680

aagatggtgg tcattatgat gctgaagtta aaactactta taaagctaaa aaaccagttc     7740

aattaccagg tgcttataat gttaatatta aattggatat tacttcacat aatgaagatt     7800

atactattgt tgaacaatat gaaagagctg aaggtagaca ttcaactggt ggtatggatg     7860

aattatataa ataatctaga caaatcgctc ttaaatatat acctaaagaa cattaaagct     7920

atattataag caaagatacg taaattttgc ttatattatt atacacatat catatttcta     7980

tatttttaag atttggttat ataatgtacg taatgcaaag gaaataaatt ttatacatta     8040

ttgaacagcg tccaagtaac tacattatgt gcactaatag tttagcgtcg tgaagacttt     8100

attgtgtcgc gaaaagtaaa aattttaaaa attagagcac cttgaacttg cgaaaaaggt     8160

tctcatcaac tgtttaaaag atctgagctc gcggccgcga tatcgctagc tcgagcccac     8220

acaccatagc ttcaaaatgt ttctactcct tttttactct tccagatttt ctcggactcc     8280

gcgcatcgcc gtaccacttc aaaacaccca agcacagcat actaaatttt ccctctttct     8340

tcctctaggg tgtcgttaat tacccgtact aaaggtttgg aaaagaaaaa agagaccgcc     8400

tcgtttcttt ttcttcgtcg aaaaaggcaa taaaaatttt tatcacgttt ctttttcttg     8460

aaattttttt ttttagtttt tttctctttc agtgacctcc attgatattt aagttaataa     8520

acggtcttca atttctcaag tttcagtttc atttttcttg ttctattaca acttttttta     8580

cttcttgttc attagaaaga aagcatagca atctaatcta aggggcggtg ttgacaatta     8640

atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc     8700

caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt     8760

ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt     8820

ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac     8880

cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt     8940

gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg     9000

ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga     9060

gcaggactga cacgtccgac ggcggcccac gggtcccagg cctcggagat ccgtccccct     9120

tttcctttgt cgatatcatg taattagtta tgtcacgctt acattcacgc cctcccccca     9180

catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt     9240

tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc     9300

tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg     9360

gacgctcgaa ggctttaatt tgcaagctga attcccgggg at                        9402


<210> 60
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /organism="Artificial Sequence"
      /note="Gal2_Bamf"
      /mol_type="unassigned DNA"

<400> 60
cagggatcct gccgtccagg                                                20


<210> 61
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /organism="Artificial Sequence"
      /note="Gal2_Munr "
      /mol_type="unassigned DNA"

<400> 61
ctgcaattgg aagcagaggc c                                              21


<210> 62
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..25
<223> /organism="Artificial Sequence"
      /note="Gal2_Eagf"
      /mol_type="unassigned DNA"

<400> 62
gtacggccgt aaaaagggtc tttcg                                          25


