                         SEQUENCE LISTING

<110>  Novo Nordisk A/S
 
<120>  Enterokinase cleavable polypeptides

<130>  130043WO01

<160>  62    

<170>  PatentIn version 3.5

<210>  1
<211>  9
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Enterokinase cleavable fusionpolypeptide of Formula (I)


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Optional polypeptide or amino acid residue

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Optional amino acid residue selected from the genetically encoded
       amino acids

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Optional amino acid residue selected from the genetically encoded
       amino acids but S and I

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Amino acid residue selected from E, Q, L, D, G, A, S, F, H, Y, W,
       T and M

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Amino acid residue which is part of a polypeptide comprising at 
       least 2 amino acid residues, said polypeptide indicated here by 
       Xaa8-Xaa9

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Amino acid residue which is part of a polypeptide comprising at 
       least 2 amino acid residues, said polypeptide indicated here by 
       Xaa8-Xaa9

<400>  1

Xaa Xaa Xaa Xaa Gly Asp Arg Xaa Xaa 
1               5                   


<210>  2
<211>  5
<212>  PRT
<213>  Homo sapiens

<400>  2

Asp Asp Asp Asp Lys 
1               5   


<210>  3
<211>  5
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Synthetic Enterokinase cleavage site

<400>  3

Leu Lys Gly Asp Arg 
1               5   


<210>  4
<211>  31
<212>  PRT
<213>  Homo sapiens

<400>  4

His Ala Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Gly Arg Gly 
            20                  25                  30      


<210>  5
<211>  31
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Analogue of GLP-1(7-37)

<400>  5

His Ala Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
            20                  25                  30      


<210>  6
<211>  29
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Analogue of GLP-1(9-37)

<400>  6

Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly Gln Ala 
1               5                   10                  15      


Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
            20                  25                  


<210>  7
<211>  39
<212>  PRT
<213>  Heloderma suspectum

<400>  7

His Gly Glu Gly Thr Phe Thr Ser Asp Leu Ser Lys Gln Met Glu Glu 
1               5                   10                  15      


Glu Ala Val Arg Leu Phe Ile Glu Trp Leu Lys Asn Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly Ala Pro Pro Pro Ser 
        35                  


<210>  8
<211>  29
<212>  PRT
<213>  Homo sapiens

<400>  8

His Ser Gln Gly Thr Phe Thr Ser Asp Tyr Ser Lys Tyr Leu Asp Ser 
1               5                   10                  15      


Arg Arg Ala Gln Asp Phe Val Gln Trp Leu Met Asn Thr 
            20                  25                  


<210>  9
<211>  53
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Insulin precursor comprising A(1-21), B(1-29) and an AAK 
       C-peptide

<400>  9

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn Ala Ala Lys Phe Val Asn Gln His Leu Cys Gly 
            20                  25                  30          


Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys Gly Glu Arg Gly Phe 
        35                  40                  45              


Phe Tyr Thr Pro Lys 
    50              


<210>  10
<211>  5
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  N-terminal of GLP-1(7-37)

<400>  10

His Ala Glu Gly Thr 
1               5   


<210>  11
<211>  12
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Synthetic peptide substrate for Enterokinase


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Lysine(2,4-dinitrophenyl)

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Lysine(2-aminobenzoyl)amide

<400>  11

Xaa Ala Glu Gly Asp Arg His Ala Glu Gly Thr Xaa 
1               5                   10          


<210>  12
<211>  5
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Synthetic Enterokinase cleavage site

<400>  12

Ala Glu Gly Asp Arg 
1               5   


<210>  13
<211>  5
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  N-terminal of a GLP-1(9-37) analogue

<400>  13

Glu Gly Thr Phe Thr 
1               5   


<210>  14
<211>  5
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  N-terminal of Exendin-4

<400>  14

His Gly Glu Gly Thr 
1               5   


<210>  15
<211>  5
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  N-terminal of a glucagon analogue

<400>  15

His Gly Thr Phe Thr 
1               5   


<210>  16
<211>  5
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  N-terminal of a glucagon analogue

<400>  16

Gln Gly Thr Phe Thr 
1               5   


<210>  17
<211>  5
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  N-terminal of a glucagon analogue

<400>  17

His Ser Gln Gly Thr 
1               5   


<210>  18
<211>  12
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Synthetic peptide substrate for Enterokinase


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Lysine(2,4-dinitro-phenyl)

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Lysine(2-aminobenzoyl)amide

<400>  18

Xaa Ile Met Gly Asp Arg His Ala Glu Gly Thr Xaa 
1               5                   10          


<210>  19
<211>  12
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Synthetic peptide substrate for Enterokinase


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Lysine(2,4-dinitrophenyl)

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Lysine(2-aminobenzoyl)amide

<400>  19

Xaa Ile Asn Asp Asp Arg His Ala Glu Gly Thr Xaa 
1               5                   10          


<210>  20
<211>  5
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  20

Glu Glu Ala Glu Lys 
1               5   


<210>  21
<211>  5
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  21

Glu Glu Ala His Lys 
1               5   


<210>  22
<211>  7
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  22

Glu Glu Ala Glu Ala His Lys 
1               5           


<210>  23
<211>  9
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  23

Glu Glu Ala Glu Ala Glu Ala His Lys 
1               5                   


<210>  24
<211>  5
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  24

Glu Glu Gly His Lys 
1               5   


<210>  25
<211>  6
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  25

Glu Glu Gly Glu Pro Lys 
1               5       


<210>  26
<211>  7
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  26

Glu Glu Ala His Glu Leu Lys 
1               5           


<210>  27
<211>  7
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  27

Glu Glu Ala His Glu Val Lys 
1               5           


<210>  28
<211>  7
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  28

Glu Glu Ala His Glu Met Lys 
1               5           


<210>  29
<211>  7
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  29

Glu Glu Ala His Glu Phe Lys 
1               5           


<210>  30
<211>  7
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  30

Glu Glu Ala His Glu Tyr Lys 
1               5           


<210>  31
<211>  15
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  31

Glu Glu Ala His Glu Trp Lys Glu Glu Gly Asn Thr Thr Pro Lys 
1               5                   10                  15  


<210>  32
<211>  11
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  32

Glu Glu Leu Asp Ala Arg Leu Glu Ala Leu Lys 
1               5                   10      


<210>  33
<211>  6
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  33

Gln Pro Met Tyr Lys Arg 
1               5       


<210>  34
<211>  6
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  34

Gly Gln Pro Met Tyr Lys 
1               5       


<210>  35
<211>  6
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  35

Pro Gly Gln Pro Met Tyr 
1               5       


<210>  36
<211>  6
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  36

Lys Pro Gly Gln Pro Met 
1               5       


<210>  37
<211>  6
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  37

Leu Lys Pro Gly Gln Pro 
1               5       


<210>  38
<211>  6
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  38

Gln Leu Lys Pro Gly Gln 
1               5       


<210>  39
<211>  6
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  39

Leu Gln Leu Lys Pro Gly 
1               5       


<210>  40
<211>  6
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  40

Trp Leu Gln Leu Lys Pro 
1               5       


<210>  41
<211>  6
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  41

His Trp Leu Gln Leu Lys 
1               5       


<210>  42
<211>  6
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  42

Trp His Trp Leu Gln Leu 
1               5       


<210>  43
<211>  6
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  43

Ala Trp His Trp Leu Gln 
1               5       


<210>  44
<211>  6
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  44

Glu Ala Trp His Trp Leu 
1               5       


<210>  45
<211>  6
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  45

Ala Glu Ala Trp His Trp 
1               5       


<210>  46
<211>  6
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  46

Glu Ala Glu Ala Trp His 
1               5       


<210>  47
<211>  9
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  47

Asp Val Lys Pro Gly Gln Pro Leu Ala 
1               5                   


<210>  48
<211>  9
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  48

Asp Val Lys Pro Gly Gln Pro Glu Tyr 
1               5                   


<210>  49
<211>  9
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  49

Asp Val Lys Pro Gly Glu Pro Leu Tyr 
1               5                   


<210>  50
<211>  9
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  50

Asp Val Lys Pro Gly Gln Pro Leu Tyr 
1               5                   


<210>  51
<211>  9
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  51

Asp Val Lys Pro Gly Gln Pro Leu Glu 
1               5                   


<210>  52
<211>  9
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  52

Asp Val Lys Pro Gly Gln Pro Met Tyr 
1               5                   


<210>  53
<211>  9
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  53

Asp Val Lys Pro Gly Gln Glu Leu Tyr 
1               5                   


<210>  54
<211>  9
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  54

Asp Val Lys Pro Glu Gln Pro Leu Tyr 
1               5                   


<210>  55
<211>  9
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Z2 sequence

<400>  55

Asp Val Lys Glu Gly Gln Pro Leu Tyr 
1               5                   


<210>  56
<211>  5
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  X5-X4-GDR sequence

<400>  56

Asp Asp Gly Asp Arg 
1               5   


<210>  57
<211>  5
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  X5-X4-GDR sequence

<400>  57

Asp Glu Gly Asp Arg 
1               5   


<210>  58
<211>  15
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  N-terminal part of Z1 sequence derived from GLP-1(7-37)

<400>  58

His Ala Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu 
1               5                   10                  15  


<210>  59
<211>  13
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  N-terminal part of Z1 sequence derived from GLP-1(9-37)

<400>  59

Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu 
1               5                   10              


<210>  60
<211>  35
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Enterokinase cleavable polypeptide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa can be any naturally occurring amino acid

<400>  60

Xaa Asp Glu Gly Asp Arg Glu Gly Thr Phe Thr Ser Asp Val Ser Ser 
1               5                   10                  15      


Tyr Leu Glu Gly Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg 
            20                  25                  30          


Gly Arg Gly 
        35  


<210>  61
<211>  35
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Enterokinase cleavable polypeptide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa can be any naturally occurring amino acid

<400>  61

Xaa Asp Asp Asp Asp Lys Glu Gly Thr Phe Thr Ser Asp Val Ser Ser 
1               5                   10                  15      


Tyr Leu Glu Gly Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg 
            20                  25                  30          


Gly Arg Gly 
        35  


<210>  62
<211>  43
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  Enterokinase cleavable polypeptide

<400>  62

Asp Val Lys Pro Gly Gln Pro Leu Tyr Asp Glu Gly Asp Arg Glu Gly 
1               5                   10                  15      


Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly Gln Ala Ala Lys 
            20                  25                  30          


Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
        35                  40              


