                         SEQUENCE LISTING

<110>  F. Hoffmann-La Roche AG
 
<120>  Improved recombinant polypeptide production methods

<130>   P31357-WO

<150>  EP13198738.0
<151>  2013-12-20

<160>  109   

<170>  PatentIn version 3.5

<210>  1
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GlySer-linker

<400>  1

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10      


<210>  2
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  enhanced green fluorescent protein

<400>  2

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 


<210>  3
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  emerald green fluorescent protein

<400>  3

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Tyr Gly Val Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Lys Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Val Asn Phe Lys Thr Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 


<210>  4
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  tagGFP

<400>  4

Met Ser Gly Gly Glu Glu Leu Phe Ala Gly Ile Val Pro Val Leu Ile 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val His Gly His Lys Phe Ser Val Arg Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Asp Tyr Gly Lys Leu Glu Ile Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 
    50                  55                  60                  


Cys Tyr Gly Ile Gln Cys Phe Ala Arg Tyr Pro Glu His Met Lys Met 
65                  70                  75                  80  


Asn Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Ile Gln Glu Arg 
                85                  90                  95      


Thr Ile Gln Phe Gln Asp Asp Gly Lys Tyr Lys Thr Arg Gly Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Lys 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Ser 
    130                 135                 140                 


Phe Asn Ser His Asn Val Tyr Ile Arg Pro Asp Lys Ala Asn Asn Gly 
145                 150                 155                 160 


Leu Glu Ala Asn Phe Lys Thr Arg His Asn Ile Glu Gly Gly Gly Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Thr Asn Val Pro Leu Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Ile Pro Ile Asn His Tyr Leu Ser Thr Gln Thr Lys Ile Ser 
        195                 200                 205             


Lys Asp Arg Asn Glu Ala Arg Asp His Met Val Leu Leu Glu Ser Phe 
    210                 215                 220                 


Ser Ala Cys Cys His Thr His Gly Met Asp Glu Leu Tyr Arg 
225                 230                 235             


<210>  5
<211>  448
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-IGF-1R antibody HC

<400>  5

Gln Val Glu Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Gln Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Ile Ile Trp Phe Asp Gly Ser Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Arg Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Glu Leu Gly Arg Arg Tyr Phe Asp Leu Trp Gly Arg Gly Thr 
            100                 105                 110         


Leu Val Ser Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr 
    210                 215                 220                 


His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser 
225                 230                 235                 240 


Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 
                245                 250                 255     


Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 
            260                 265                 270         


Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
        275                 280                 285             


Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val 
    290                 295                 300                 


Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
305                 310                 315                 320 


Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 
                325                 330                 335     


Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
            340                 345                 350         


Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys 
        355                 360                 365             


Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
    370                 375                 380                 


Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 
385                 390                 395                 400 


Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 
                405                 410                 415     


Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
            420                 425                 430         


Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        435                 440                 445             


<210>  6
<211>  215
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-IGF-1R antibody LC

<400>  6

Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Tyr 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile 
        35                  40                  45              


Tyr Asp Ala Ser Lys Arg Ala Thr Gly Ile Pro Ala Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Arg Ser Lys Trp Pro Pro 
                85                  90                  95      


Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ser Lys Arg Thr Val Ala 
            100                 105                 110         


Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser 
        115                 120                 125             


Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu 
    130                 135                 140                 


Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser 
145                 150                 155                 160 


Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu 
                165                 170                 175     


Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val 
            180                 185                 190         


Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys 
        195                 200                 205             


Ser Phe Asn Arg Gly Glu Cys 
    210                 215 


<210>  7
<211>  715
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-IGF-1R antibody HC - eGFP fusion polypeptide

<400>  7

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Gln Val Glu Leu Val Glu Ser Gly Gly Gly Val Val Gln 
            20                  25                  30          


Pro Gly Arg Ser Gln Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
        35                  40                  45              


Ser Ser Tyr Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    50                  55                  60                  


Glu Trp Val Ala Ile Ile Trp Phe Asp Gly Ser Ser Thr Tyr Tyr Ala 
65                  70                  75                  80  


Asp Ser Val Arg Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn 
                85                  90                  95      


Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Phe Cys Ala Arg Glu Leu Gly Arg Arg Tyr Phe Asp Leu Trp Gly 
        115                 120                 125             


Arg Gly Thr Leu Val Ser Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 
    130                 135                 140                 


Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 
145                 150                 155                 160 


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
                165                 170                 175     


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
            180                 185                 190         


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
        195                 200                 205             


Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His 
    210                 215                 220                 


Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys 
225                 230                 235                 240 


Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
                245                 250                 255     


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            260                 265                 270         


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        275                 280                 285             


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    290                 295                 300                 


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
305                 310                 315                 320 


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                325                 330                 335     


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            340                 345                 350         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        355                 360                 365             


Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 
    370                 375                 380                 


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
385                 390                 395                 400 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                405                 410                 415     


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            420                 425                 430         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        435                 440                 445             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    450                 455                 460                 


Pro Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Met Val Ser Lys 
465                 470                 475                 480 


Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp 
                485                 490                 495     


Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly 
            500                 505                 510         


Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly 
        515                 520                 525             


Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly 
    530                 535                 540                 


Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe 
545                 550                 555                 560 


Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe 
                565                 570                 575     


Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu 
            580                 585                 590         


Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys 
        595                 600                 605             


Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser 
    610                 615                 620                 


His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val 
625                 630                 635                 640 


Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala 
                645                 650                 655     


Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu 
            660                 665                 670         


Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro 
        675                 680                 685             


Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala 
    690                 695                 700                 


Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
705                 710                 715 


<210>  8
<211>  715
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-IGF-1R antibody HC - emGFP fusion polypeptide

<400>  8

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Gln Val Glu Leu Val Glu Ser Gly Gly Gly Val Val Gln 
            20                  25                  30          


Pro Gly Arg Ser Gln Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
        35                  40                  45              


Ser Ser Tyr Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    50                  55                  60                  


Glu Trp Val Ala Ile Ile Trp Phe Asp Gly Ser Ser Thr Tyr Tyr Ala 
65                  70                  75                  80  


Asp Ser Val Arg Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn 
                85                  90                  95      


Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Phe Cys Ala Arg Glu Leu Gly Arg Arg Tyr Phe Asp Leu Trp Gly 
        115                 120                 125             


Arg Gly Thr Leu Val Ser Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 
    130                 135                 140                 


Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 
145                 150                 155                 160 


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
                165                 170                 175     


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
            180                 185                 190         


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
        195                 200                 205             


Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His 
    210                 215                 220                 


Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys 
225                 230                 235                 240 


Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
                245                 250                 255     


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            260                 265                 270         


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        275                 280                 285             


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    290                 295                 300                 


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
305                 310                 315                 320 


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                325                 330                 335     


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            340                 345                 350         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        355                 360                 365             


Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 
    370                 375                 380                 


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
385                 390                 395                 400 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                405                 410                 415     


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            420                 425                 430         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        435                 440                 445             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    450                 455                 460                 


Pro Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Met Val Ser Lys 
465                 470                 475                 480 


Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp 
                485                 490                 495     


Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly 
            500                 505                 510         


Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly 
        515                 520                 525             


Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly 
    530                 535                 540                 


Val Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln His Asp Phe 
545                 550                 555                 560 


Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe 
                565                 570                 575     


Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu 
            580                 585                 590         


Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys 
        595                 600                 605             


Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser 
    610                 615                 620                 


His Lys Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly Ile Lys Val 
625                 630                 635                 640 


Asn Phe Lys Thr Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala 
                645                 650                 655     


Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu 
            660                 665                 670         


Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro 
        675                 680                 685             


Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala 
    690                 695                 700                 


Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
705                 710                 715 


<210>  9
<211>  714
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-IGF-1R antibody HC - tagGFP fusion polypeptide

<400>  9

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Gln Val Glu Leu Val Glu Ser Gly Gly Gly Val Val Gln 
            20                  25                  30          


Pro Gly Arg Ser Gln Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
        35                  40                  45              


Ser Ser Tyr Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    50                  55                  60                  


Glu Trp Val Ala Ile Ile Trp Phe Asp Gly Ser Ser Thr Tyr Tyr Ala 
65                  70                  75                  80  


Asp Ser Val Arg Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn 
                85                  90                  95      


Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Phe Cys Ala Arg Glu Leu Gly Arg Arg Tyr Phe Asp Leu Trp Gly 
        115                 120                 125             


Arg Gly Thr Leu Val Ser Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 
    130                 135                 140                 


Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 
145                 150                 155                 160 


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
                165                 170                 175     


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
            180                 185                 190         


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
        195                 200                 205             


Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His 
    210                 215                 220                 


Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys 
225                 230                 235                 240 


Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
                245                 250                 255     


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            260                 265                 270         


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        275                 280                 285             


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    290                 295                 300                 


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
305                 310                 315                 320 


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                325                 330                 335     


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            340                 345                 350         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        355                 360                 365             


Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 
    370                 375                 380                 


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
385                 390                 395                 400 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                405                 410                 415     


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            420                 425                 430         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        435                 440                 445             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    450                 455                 460                 


Pro Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Met Ser Gly Gly 
465                 470                 475                 480 


Glu Glu Leu Phe Ala Gly Ile Val Pro Val Leu Ile Glu Leu Asp Gly 
                485                 490                 495     


Asp Val His Gly His Lys Phe Ser Val Arg Gly Glu Gly Glu Gly Asp 
            500                 505                 510         


Ala Asp Tyr Gly Lys Leu Glu Ile Lys Phe Ile Cys Thr Thr Gly Lys 
        515                 520                 525             


Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Cys Tyr Gly Ile 
    530                 535                 540                 


Gln Cys Phe Ala Arg Tyr Pro Glu His Met Lys Met Asn Asp Phe Phe 
545                 550                 555                 560 


Lys Ser Ala Met Pro Glu Gly Tyr Ile Gln Glu Arg Thr Ile Gln Phe 
                565                 570                 575     


Gln Asp Asp Gly Lys Tyr Lys Thr Arg Gly Glu Val Lys Phe Glu Gly 
            580                 585                 590         


Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Lys Asp Phe Lys Glu 
        595                 600                 605             


Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Ser Phe Asn Ser His 
    610                 615                 620                 


Asn Val Tyr Ile Arg Pro Asp Lys Ala Asn Asn Gly Leu Glu Ala Asn 
625                 630                 635                 640 


Phe Lys Thr Arg His Asn Ile Glu Gly Gly Gly Val Gln Leu Ala Asp 
                645                 650                 655     


His Tyr Gln Thr Asn Val Pro Leu Gly Asp Gly Pro Val Leu Ile Pro 
            660                 665                 670         


Ile Asn His Tyr Leu Ser Thr Gln Thr Lys Ile Ser Lys Asp Arg Asn 
        675                 680                 685             


Glu Ala Arg Asp His Met Val Leu Leu Glu Ser Phe Ser Ala Cys Cys 
    690                 695                 700                 


His Thr His Gly Met Asp Glu Leu Tyr Arg 
705                 710                 


<210>  10
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human opsin receptor derived polypeptide = opsin-tag

<400>  10

Asn Gly Thr Glu Gly Pro Asn Phe Tyr Val Pro Phe Ser Asn Ala Thr 
1               5                   10                  15      


Gly Val Val 
            


<210>  11
<211>  734
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-IGF-1R antibody HC eGFP opsin-tag fusion polypeptide

<400>  11

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Gln Val Glu Leu Val Glu Ser Gly Gly Gly Val Val Gln 
            20                  25                  30          


Pro Gly Arg Ser Gln Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
        35                  40                  45              


Ser Ser Tyr Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    50                  55                  60                  


Glu Trp Val Ala Ile Ile Trp Phe Asp Gly Ser Ser Thr Tyr Tyr Ala 
65                  70                  75                  80  


Asp Ser Val Arg Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn 
                85                  90                  95      


Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Phe Cys Ala Arg Glu Leu Gly Arg Arg Tyr Phe Asp Leu Trp Gly 
        115                 120                 125             


Arg Gly Thr Leu Val Ser Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 
    130                 135                 140                 


Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 
145                 150                 155                 160 


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
                165                 170                 175     


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
            180                 185                 190         


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
        195                 200                 205             


Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His 
    210                 215                 220                 


Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys 
225                 230                 235                 240 


Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
                245                 250                 255     


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            260                 265                 270         


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        275                 280                 285             


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    290                 295                 300                 


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
305                 310                 315                 320 


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                325                 330                 335     


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            340                 345                 350         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        355                 360                 365             


Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 
    370                 375                 380                 


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
385                 390                 395                 400 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                405                 410                 415     


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            420                 425                 430         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        435                 440                 445             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    450                 455                 460                 


Pro Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Met Val Ser Lys 
465                 470                 475                 480 


Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp 
                485                 490                 495     


Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly 
            500                 505                 510         


Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly 
        515                 520                 525             


Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly 
    530                 535                 540                 


Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe 
545                 550                 555                 560 


Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe 
                565                 570                 575     


Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu 
            580                 585                 590         


Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys 
        595                 600                 605             


Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser 
    610                 615                 620                 


His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val 
625                 630                 635                 640 


Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala 
                645                 650                 655     


Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu 
            660                 665                 670         


Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro 
        675                 680                 685             


Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala 
    690                 695                 700                 


Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Asn Gly Thr Glu Gly 
705                 710                 715                 720 


Pro Asn Phe Tyr Val Pro Phe Ser Asn Ala Thr Gly Val Val 
                725                 730                 


<210>  12
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  T7-His6 tag

<400>  12

Met Ala Ser Met Thr Gly Gly Gln Gln Met Gly His His His His His 
1               5                   10                  15      


His 
    


<210>  13
<211>  267
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro-BDNF-T7-His6

<400>  13

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Arg Gly Arg Gly Ser Gly Met Ala Ser Met Thr Gly 
                245                 250                 255     


Gly Gln Gln Met Gly His His His His His His 
            260                 265         


<210>  14
<211>  19
<212>  PRT
<213>  Mus musculus

<400>  14

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser 
            


<210>  15
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  short opsin-tag

<400>  15

Asn Gly Thr Glu Gly Pro Asn Phe Tyr Val Pro Phe Ser Asn Ala Thr 
1               5                   10                  15      


<210>  16
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  long opsin-tag

<400>  16

Asn Gly Thr Glu Gly Pro Asn Phe Tyr Val Pro Phe Ser Asn Ala Thr 
1               5                   10                  15      


Gly Val Val Arg 
            20  


<210>  17
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  glycosylation-tag

<400>  17

Ala Ala Ala Asn Gly Thr Gly Gly Ala 
1               5                   


<210>  18
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  glycosylation-tag

<400>  18

Ala Asn Ile Thr Val Asn Ile Thr Val 
1               5                   


<210>  19
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  glycosylation-tag

<400>  19

Asn Ala Thr Gly Ala Asp Asn Gly Thr Gly Ala Ser 
1               5                   10          


<210>  20
<211>  247
<212>  PRT
<213>  Homo sapiens

<400>  20

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Arg Gly Arg 
                245         


<210>  21
<211>  4
<212>  PRT
<213>  Homo sapiens

<400>  21

Arg Val Arg Arg 
1               


<210>  22
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgA protease cleavage sequence

<400>  22

Gly Ser Val Val Ala Pro Pro Ala Pro 
1               5                   


<210>  23
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His6-tag

<400>  23

His His His His His His 
1               5       


<210>  24
<211>  3
<212>  PRT
<213>  Homo sapiens

<400>  24

Arg Gly Arg 
1           


<210>  25
<211>  119
<212>  PRT
<213>  Homo sapiens

<400>  25

His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
1               5                   10                  15      


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
            20                  25                  30          


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
        35                  40                  45              


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
    50                  55                  60                  


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
65                  70                  75                  80  


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
                85                  90                  95      


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
            100                 105                 110         


Leu Thr Ile Lys Arg Gly Arg 
        115                 


<210>  26
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker peptide

<400>  26

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
1               5                   10          


<210>  27
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker peptide

<400>  27

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly 
            20          


<210>  28
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker peptide

<400>  28

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            20                  25                  30          


<210>  29
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker peptide

<400>  29

Gly Gly Gly Asp Gly Gly Gly Asp Gly Gly Gly Asp Gly Gly Gly Asp 
1               5                   10                  15      


Gly Gly Gly Asp Gly Gly Gly Ser 
            20                  


<210>  30
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker peptide

<400>  30

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  31
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker peptide

<400>  31

Gly Gly Asp Gly Gly Asp Gly Gly Asp Gly Gly Asp Gly Gly Asp Gly 
1               5                   10                  15      


Gly Ser Gly 
            


<210>  32
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker peptide

<400>  32

Gly Gly Asp Gly Gly Asp Gly Gly Asp Gly Gly Asp Gly Gly Asp Gly 
1               5                   10                  15      


Gly Gly Ser 
            


<210>  33
<211>  734
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HC<IGF-1R>_(G4S)2G_eGFP-2N

<400>  33

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Gln Val Glu Leu Val Glu Ser Gly Gly Gly Val Val Gln 
            20                  25                  30          


Pro Gly Arg Ser Gln Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
        35                  40                  45              


Ser Ser Tyr Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    50                  55                  60                  


Glu Trp Val Ala Ile Ile Trp Phe Asp Gly Ser Ser Thr Tyr Tyr Ala 
65                  70                  75                  80  


Asp Ser Val Arg Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn 
                85                  90                  95      


Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Phe Cys Ala Arg Glu Leu Gly Arg Arg Tyr Phe Asp Leu Trp Gly 
        115                 120                 125             


Arg Gly Thr Leu Val Ser Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 
    130                 135                 140                 


Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 
145                 150                 155                 160 


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
                165                 170                 175     


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
            180                 185                 190         


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
        195                 200                 205             


Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His 
    210                 215                 220                 


Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys 
225                 230                 235                 240 


Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
                245                 250                 255     


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            260                 265                 270         


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        275                 280                 285             


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    290                 295                 300                 


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
305                 310                 315                 320 


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                325                 330                 335     


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            340                 345                 350         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        355                 360                 365             


Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 
    370                 375                 380                 


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
385                 390                 395                 400 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                405                 410                 415     


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            420                 425                 430         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        435                 440                 445             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    450                 455                 460                 


Pro Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Met Val Ser Lys 
465                 470                 475                 480 


Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp 
                485                 490                 495     


Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly 
            500                 505                 510         


Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly 
        515                 520                 525             


Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly 
    530                 535                 540                 


Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe 
545                 550                 555                 560 


Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe 
                565                 570                 575     


Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu 
            580                 585                 590         


Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys 
        595                 600                 605             


Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser 
    610                 615                 620                 


His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val 
625                 630                 635                 640 


Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala 
                645                 650                 655     


Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu 
            660                 665                 670         


Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro 
        675                 680                 685             


Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala 
    690                 695                 700                 


Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Asn Gly Thr Glu Gly 
705                 710                 715                 720 


Pro Asn Phe Tyr Val Pro Phe Ser Asn Ala Thr Gly Val Val 
                725                 730                 


<210>  34
<211>  267
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pro (wt) - mBDNF (wt) - T7 - His6

<400>  34

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Arg Gly Arg Gly Ser Gly Met Ala Ser Met Thr Gly 
                245                 250                 255     


Gly Gln Gln Met Gly His His His His His His 
            260                 265         


<210>  35
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pro (wt) - mBDNF (deltaRGR) (wt) - T7 - His6

<400>  35

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  36
<211>  283
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  proBDNF (deltaRGR)-GSG-2N-GSG-T7-His6 (opsin-S)

<400>  36

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Asn Gly Thr Glu Gly Pro Asn Phe Tyr 
                245                 250                 255     


Val Pro Phe Ser Asn Ala Thr Gly Ser Gly Met Ala Ser Met Thr Gly 
            260                 265                 270         


Gly Gln Gln Met Gly His His His His His His 
        275                 280             


<210>  37
<211>  272
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  proBDNF (deltaRGR)-GSG-2N-GSG-His6 (opsin-S)

<400>  37

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Asn Gly Thr Glu Gly Pro Asn Phe Tyr 
                245                 250                 255     


Val Pro Phe Ser Asn Ala Thr Gly Ser Gly His His His His His His 
            260                 265                 270         


<210>  38
<211>  273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  proBDNF (deltaRGR)-GSG-2N-GSG-His6 (opsin-L

<400>  38

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Asn Gly Thr Glu Gly Pro Asn Phe Tyr 
                245                 250                 255     


Val Pro Phe Ser Asn Ala Thr Gly Val Val Arg His His His His His 
            260                 265                 270         


His 
    


<210>  39
<211>  265
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  proBDNF - GSG - 2NIT - His6

<400>  39

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Ala Asn Ile Thr Val Asn Ile Thr Val 
                245                 250                 255     


Gly Ser Gly His His His His His His 
            260                 265 


<210>  40
<211>  262
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  proBDNF - GSG - NGT - His6

<400>  40

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Ala Ala Ala Asn Gly Thr Gly Gly Ala 
                245                 250                 255     


His His His His His His 
            260         


<210>  41
<211>  265
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  proBDNF - GSG - 2NAT - His6

<400>  41

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Asn Ala Thr Gly Ala Asp Asn Gly Thr 
                245                 250                 255     


Gly Ala Ser His His His His His His 
            260                 265 


<210>  42
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pre-pro-BDNF(-RGR; M61T)-T7-His6

<400>  42

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Thr Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  43
<211>  258
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pro(BDNF) - IgA-Site - mBDNF - GSG - His6

<400>  43

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Gly Ser Val Val 
        115                 120                 125             


Ala Pro Pro Ala Pro His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser 
    130                 135                 140                 


Val Cys Asp Ser Ile Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr 
145                 150                 155                 160 


Ala Val Asp Met Ser Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro 
                165                 170                 175     


Val Ser Lys Gly Gln Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn 
            180                 185                 190         


Pro Met Gly Tyr Thr Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His 
        195                 200                 205             


Trp Asn Ser Gln Cys Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr 
    210                 215                 220                 


Met Asp Ser Lys Lys Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr 
225                 230                 235                 240 


Ser Cys Val Cys Thr Leu Thr Ile Lys Gly Ser Gly His His His His 
                245                 250                 255     


His His 
        


<210>  44
<211>  253
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pro(BDNF) R54A -mBDNF - GSG  - His6

<400>  44

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Ala Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly His His His His His His 
                245                 250             


<210>  45
<211>  263
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His6 - pro(BDNF) R54A - IgA-Site - mBDNF

<400>  45

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile His His His His His His 
            20                  25                  30          


Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu Ala Tyr 
        35                  40                  45              


Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly Pro Lys 
    50                  55                  60                  


Ala Gly Ser Ala Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu His Val 
65                  70                  75                  80  


Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn Glu Glu 
                85                  90                  95      


Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu Ser Ser 
            100                 105                 110         


Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu Tyr Lys 
        115                 120                 125             


Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Gly Ser Val Val Ala Pro 
    130                 135                 140                 


Pro Ala Pro His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys 
145                 150                 155                 160 


Asp Ser Ile Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val 
                165                 170                 175     


Asp Met Ser Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser 
            180                 185                 190         


Lys Gly Gln Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met 
        195                 200                 205             


Gly Tyr Thr Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn 
    210                 215                 220                 


Ser Gln Cys Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp 
225                 230                 235                 240 


Ser Lys Lys Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys 
                245                 250                 255     


Val Cys Thr Leu Thr Ile Lys 
            260             


<210>  46
<211>  258
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His6 - pro(BDNF) R54A - mBDNF

<400>  46

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile His His His His His His 
            20                  25                  30          


Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu Ala Tyr 
        35                  40                  45              


Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly Pro Lys 
    50                  55                  60                  


Ala Gly Ser Ala Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu His Val 
65                  70                  75                  80  


Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn Glu Glu 
                85                  90                  95      


Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu Ser Ser 
            100                 105                 110         


Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu Tyr Lys 
        115                 120                 125             


Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg His Ser 
    130                 135                 140                 


Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile Ser Glu 
145                 150                 155                 160 


Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser Gly Gly 
                165                 170                 175     


Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln Leu Lys 
            180                 185                 190         


Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr Lys Glu 
        195                 200                 205             


Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys Arg Thr 
    210                 215                 220                 


Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys Arg Ile 
225                 230                 235                 240 


Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr Leu Thr 
                245                 250                 255     


Ile Lys 
        


<210>  47
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ProBDNF P60E, K65D, K73D, K95A - GSG - T7 - His6

<400>  47

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Glu Met Gly Tyr Thr 
            180                 185                 190         


Asp Glu Gly Cys Arg Gly Ile Asp Asp Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Ala Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  48
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ProBDNF K65D, K73D, K95A, R97A - GSG - T7 - His6

<400>  48

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Asp Glu Gly Cys Arg Gly Ile Asp Asp Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Ala Lys 
    210                 215                 220                 


Ala Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  49
<211>  266
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ProBDNF - GSG - myc - His6

<400>  49

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Glu Gln Lys Leu Ile Ser Glu Glu Asp 
                245                 250                 255     


Leu Gly Ser Gly His His His His His His 
            260                 265     


<210>  50
<211>  278
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro(IgA)-BDNF(-RGR; R81N)-opsin(L)

<400>  50

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Gly Ser Val Val 
        115                 120                 125             


Ala Pro Pro Ala Pro His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser 
    130                 135                 140                 


Val Cys Asp Ser Ile Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr 
145                 150                 155                 160 


Ala Val Asp Met Ser Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro 
                165                 170                 175     


Val Ser Lys Gly Gln Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn 
            180                 185                 190         


Pro Met Gly Tyr Thr Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His 
        195                 200                 205             


Trp Asn Ser Gln Cys Asn Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr 
    210                 215                 220                 


Met Asp Ser Lys Lys Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr 
225                 230                 235                 240 


Ser Cys Val Cys Thr Leu Thr Ile Lys Gly Ser Gly Asn Gly Thr Glu 
                245                 250                 255     


Gly Pro Asn Phe Tyr Val Pro Phe Ser Asn Ala Thr Gly Val Val Arg 
            260                 265                 270         


His His His His His His 
        275             


<210>  51
<211>  294
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro(IgA)-BDNF(-RGR; R81N)-opsin(L)-myc-His6

<400>  51

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Gly Ser Val Val 
        115                 120                 125             


Ala Pro Pro Ala Pro His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser 
    130                 135                 140                 


Val Cys Asp Ser Ile Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr 
145                 150                 155                 160 


Ala Val Asp Met Ser Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro 
                165                 170                 175     


Val Ser Lys Gly Gln Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn 
            180                 185                 190         


Pro Met Gly Tyr Thr Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His 
        195                 200                 205             


Trp Asn Ser Gln Cys Asn Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr 
    210                 215                 220                 


Met Asp Ser Lys Lys Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr 
225                 230                 235                 240 


Ser Cys Val Cys Thr Leu Thr Ile Lys Gly Ser Gly Asn Gly Thr Glu 
                245                 250                 255     


Gly Pro Asn Phe Tyr Val Pro Phe Ser Asn Ala Thr Gly Val Val Arg 
            260                 265                 270         


Gly Ser Gly Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Gly Ser Gly 
        275                 280                 285             


His His His His His His 
    290                 


<210>  52
<211>  268
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro(IgA)-BDNF(-RGR; R81N)-myc-His6

<400>  52

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Gly Ser Val Val 
        115                 120                 125             


Ala Pro Pro Ala Pro His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser 
    130                 135                 140                 


Val Cys Asp Ser Ile Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr 
145                 150                 155                 160 


Ala Val Asp Met Ser Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro 
                165                 170                 175     


Val Ser Lys Gly Gln Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn 
            180                 185                 190         


Pro Met Gly Tyr Thr Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His 
        195                 200                 205             


Trp Asn Ser Gln Cys Asn Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr 
    210                 215                 220                 


Met Asp Ser Lys Lys Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr 
225                 230                 235                 240 


Ser Cys Val Cys Thr Leu Thr Ile Lys Gly Ser Gly Glu Gln Lys Leu 
                245                 250                 255     


Ile Ser Glu Glu Asp Leu His His His His His His 
            260                 265             


<210>  53
<211>  258
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro(IgA)-BDNF(-RGR; R81N)-His6

<400>  53

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Gly Ser Val Val 
        115                 120                 125             


Ala Pro Pro Ala Pro His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser 
    130                 135                 140                 


Val Cys Asp Ser Ile Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr 
145                 150                 155                 160 


Ala Val Asp Met Ser Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro 
                165                 170                 175     


Val Ser Lys Gly Gln Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn 
            180                 185                 190         


Pro Met Gly Tyr Thr Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His 
        195                 200                 205             


Trp Asn Ser Gln Cys Asn Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr 
    210                 215                 220                 


Met Asp Ser Lys Lys Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr 
225                 230                 235                 240 


Ser Cys Val Cys Thr Leu Thr Ile Lys Gly Ser Gly His His His His 
                245                 250                 255     


His His 
        


<210>  54
<211>  278
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro(IgA)-BDNF(-RGR)-opsin(L)-His6

<400>  54

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Gly Ser Val Val 
        115                 120                 125             


Ala Pro Pro Ala Pro His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser 
    130                 135                 140                 


Val Cys Asp Ser Ile Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr 
145                 150                 155                 160 


Ala Val Asp Met Ser Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro 
                165                 170                 175     


Val Ser Lys Gly Gln Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn 
            180                 185                 190         


Pro Met Gly Tyr Thr Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His 
        195                 200                 205             


Trp Asn Ser Gln Cys Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr 
    210                 215                 220                 


Met Asp Ser Lys Lys Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr 
225                 230                 235                 240 


Ser Cys Val Cys Thr Leu Thr Ile Lys Gly Ser Gly Asn Gly Thr Glu 
                245                 250                 255     


Gly Pro Asn Phe Tyr Val Pro Phe Ser Asn Ala Thr Gly Val Val Arg 
            260                 265                 270         


His His His His His His 
        275             


<210>  55
<211>  278
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro(IgA)-BDNF(-RGR; K25N)-opsin(L)-His6

<400>  55

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Gly Ser Val Val 
        115                 120                 125             


Ala Pro Pro Ala Pro His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser 
    130                 135                 140                 


Val Cys Asp Ser Ile Ser Glu Trp Val Thr Ala Ala Asp Asn Lys Thr 
145                 150                 155                 160 


Ala Val Asp Met Ser Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro 
                165                 170                 175     


Val Ser Lys Gly Gln Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn 
            180                 185                 190         


Pro Met Gly Tyr Thr Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His 
        195                 200                 205             


Trp Asn Ser Gln Cys Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr 
    210                 215                 220                 


Met Asp Ser Lys Lys Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr 
225                 230                 235                 240 


Ser Cys Val Cys Thr Leu Thr Ile Lys Gly Ser Gly Asn Gly Thr Glu 
                245                 250                 255     


Gly Pro Asn Phe Tyr Val Pro Phe Ser Asn Ala Thr Gly Val Val Arg 
            260                 265                 270         


His His His His His His 
        275             


<210>  56
<211>  278
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro(IgA)-BDNF(-RGR; T35N)-opsin(L)-His6

<400>  56

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Gly Ser Val Val 
        115                 120                 125             


Ala Pro Pro Ala Pro His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser 
    130                 135                 140                 


Val Cys Asp Ser Ile Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr 
145                 150                 155                 160 


Ala Val Asp Met Ser Gly Gly Asn Val Thr Val Leu Glu Lys Val Pro 
                165                 170                 175     


Val Ser Lys Gly Gln Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn 
            180                 185                 190         


Pro Met Gly Tyr Thr Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His 
        195                 200                 205             


Trp Asn Ser Gln Cys Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr 
    210                 215                 220                 


Met Asp Ser Lys Lys Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr 
225                 230                 235                 240 


Ser Cys Val Cys Thr Leu Thr Ile Lys Gly Ser Gly Asn Gly Thr Glu 
                245                 250                 255     


Gly Pro Asn Phe Tyr Val Pro Phe Ser Asn Ala Thr Gly Val Val Arg 
            260                 265                 270         


His His His His His His 
        275             


<210>  57
<211>  278
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro(IgA)-BDNF(-RGR; K25N, T35N)-opsin(L)-His6

<400>  57

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Gly Ser Val Val 
        115                 120                 125             


Ala Pro Pro Ala Pro His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser 
    130                 135                 140                 


Val Cys Asp Ser Ile Ser Glu Trp Val Thr Ala Ala Asp Asn Lys Thr 
145                 150                 155                 160 


Ala Val Asp Met Ser Gly Gly Asn Val Thr Val Leu Glu Lys Val Pro 
                165                 170                 175     


Val Ser Lys Gly Gln Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn 
            180                 185                 190         


Pro Met Gly Tyr Thr Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His 
        195                 200                 205             


Trp Asn Ser Gln Cys Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr 
    210                 215                 220                 


Met Asp Ser Lys Lys Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr 
225                 230                 235                 240 


Ser Cys Val Cys Thr Leu Thr Ile Lys Gly Ser Gly Asn Gly Thr Glu 
                245                 250                 255     


Gly Pro Asn Phe Tyr Val Pro Phe Ser Asn Ala Thr Gly Val Val Arg 
            260                 265                 270         


His His His His His His 
        275             


<210>  58
<211>  269
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro(IgA)-BDNF(-RGR; K25N, T35N, R81N)-T7-His6

<400>  58

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Gly Ser Val Val 
        115                 120                 125             


Ala Pro Pro Ala Pro His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser 
    130                 135                 140                 


Val Cys Asp Ser Ile Ser Glu Trp Val Thr Ala Ala Asp Asn Lys Thr 
145                 150                 155                 160 


Ala Val Asp Met Ser Gly Gly Asn Val Thr Val Leu Glu Lys Val Pro 
                165                 170                 175     


Val Ser Lys Gly Gln Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn 
            180                 185                 190         


Pro Met Gly Tyr Thr Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His 
        195                 200                 205             


Trp Asn Ser Gln Cys Asn Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr 
    210                 215                 220                 


Met Asp Ser Lys Lys Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr 
225                 230                 235                 240 


Ser Cys Val Cys Thr Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met 
                245                 250                 255     


Thr Gly Gly Gln Gln Met Gly His His His His His His 
            260                 265                 


<210>  59
<211>  269
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro(IgA)-BDNF(-RGR; K25N, T35N, Q79T, R81N)-T7-His6

<400>  59

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Gly Ser Val Val 
        115                 120                 125             


Ala Pro Pro Ala Pro His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser 
    130                 135                 140                 


Val Cys Asp Ser Ile Ser Glu Trp Val Thr Ala Ala Asp Asn Lys Thr 
145                 150                 155                 160 


Ala Val Asp Met Ser Gly Gly Asn Val Thr Val Leu Glu Lys Val Pro 
                165                 170                 175     


Val Ser Lys Gly Gln Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn 
            180                 185                 190         


Pro Met Gly Tyr Thr Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His 
        195                 200                 205             


Trp Asn Ser Thr Cys Asn Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr 
    210                 215                 220                 


Met Asp Ser Lys Lys Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr 
225                 230                 235                 240 


Ser Cys Val Cys Thr Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met 
                245                 250                 255     


Thr Gly Gly Gln Gln Met Gly His His His His His His 
            260                 265                 


<210>  60
<211>  278
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro(IgA)-BDNF(-RGR; G62N, Y63G)-opsin(L)-His6

<400>  60

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Gly Ser Val Val 
        115                 120                 125             


Ala Pro Pro Ala Pro His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser 
    130                 135                 140                 


Val Cys Asp Ser Ile Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr 
145                 150                 155                 160 


Ala Val Asp Met Ser Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro 
                165                 170                 175     


Val Ser Lys Gly Gln Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn 
            180                 185                 190         


Pro Met Asn Gly Thr Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His 
        195                 200                 205             


Trp Asn Ser Gln Cys Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr 
    210                 215                 220                 


Met Asp Ser Lys Lys Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr 
225                 230                 235                 240 


Ser Cys Val Cys Thr Leu Thr Ile Lys Gly Ser Gly Asn Gly Thr Glu 
                245                 250                 255     


Gly Pro Asn Phe Tyr Val Pro Phe Ser Asn Ala Thr Gly Val Val Arg 
            260                 265                 270         


His His His His His His 
        275             


<210>  61
<211>  278
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro(IgA)-BDNF(-RGR; G62N, Y63G, K25N, T35N)-opsin(L)-His6

<400>  61

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Gly Ser Val Val 
        115                 120                 125             


Ala Pro Pro Ala Pro His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser 
    130                 135                 140                 


Val Cys Asp Ser Ile Ser Glu Trp Val Thr Ala Ala Asp Asn Lys Thr 
145                 150                 155                 160 


Ala Val Asp Met Ser Gly Gly Asn Val Thr Val Leu Glu Lys Val Pro 
                165                 170                 175     


Val Ser Lys Gly Gln Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn 
            180                 185                 190         


Pro Met Asn Gly Thr Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His 
        195                 200                 205             


Trp Asn Ser Gln Cys Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr 
    210                 215                 220                 


Met Asp Ser Lys Lys Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr 
225                 230                 235                 240 


Ser Cys Val Cys Thr Leu Thr Ile Lys Gly Ser Gly Asn Gly Thr Glu 
                245                 250                 255     


Gly Pro Asn Phe Tyr Val Pro Phe Ser Asn Ala Thr Gly Val Val Arg 
            260                 265                 270         


His His His His His His 
        275             


<210>  62
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro-BDNF(-RGR; G62N, Y63G, Q79T)-T7-His6

<400>  62

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Asn Gly Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Thr Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  63
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro-BDNF(-RGR; T35N, Q79T)-T7-His6

<400>  63

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Asn Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Thr Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  64
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro-BDNF(-RGR; K25N, Q79T)-T7-His6

<400>  64

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Asn Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Thr Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  65
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro-BDNF(-RGR; T35N, G62N, Y63G, Q79T)-T7-His6

<400>  65

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Asn Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Asn Gly Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Thr Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  66
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro-BDNF(-RGR; T35N; G62N; Y63G)-T7-His6

<400>  66

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Asn Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Asn Gly Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  67
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro-BDNF(-RGR; K25N; G62N; Y63G)-T7-His6

<400>  67

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Asn Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Asn Gly Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  68
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro-BDNF(-RGR; K25N; T35N)-T7-His6

<400>  68

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Asn Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Asn Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  69
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro-BDNF(-RGR; G62N, Y63G, R81N)-T7-His6

<400>  69

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Asn Gly Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Asn Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  70
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro-BDNF(-RGR; K25N; T35N; Q79T)-T7-His6

<400>  70

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Asn Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Asn Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Asn Gly Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Thr Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  71
<211>  480
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro-BDNF(-RGD)_(G4S)2GG_VL<IGF-1R>-Ck-His6

<400>  71

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
                245                 250                 255     


Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 
            260                 265                 270         


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Tyr 
        275                 280                 285             


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile 
    290                 295                 300                 


Tyr Asp Ala Ser Lys Arg Ala Thr Gly Ile Pro Ala Arg Phe Ser Gly 
305                 310                 315                 320 


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro 
                325                 330                 335     


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Arg Ser Lys Trp Pro Pro 
            340                 345                 350         


Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ser Lys Arg Thr Val Ala 
        355                 360                 365             


Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser 
    370                 375                 380                 


Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu 
385                 390                 395                 400 


Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser 
                405                 410                 415     


Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu 
            420                 425                 430         


Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val 
        435                 440                 445             


Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys 
    450                 455                 460                 


Ser Phe Asn Arg Gly Glu Cys Gly Ser Gly His His His His His His 
465                 470                 475                 480 


<210>  72
<211>  490
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro-BDNF(-RGD)_(G4S)4GG_VL<IGF-1R>-Ck-His6

<400>  72

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
                245                 250                 255     


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Glu Ile Val Leu Thr Gln 
            260                 265                 270         


Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser 
        275                 280                 285             


Cys Arg Ala Ser Gln Ser Val Ser Ser Tyr Leu Ala Trp Tyr Gln Gln 
    290                 295                 300                 


Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile Tyr Asp Ala Ser Lys Arg 
305                 310                 315                 320 


Ala Thr Gly Ile Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
                325                 330                 335     


Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro Glu Asp Phe Ala Val Tyr 
            340                 345                 350         


Tyr Cys Gln Gln Arg Ser Lys Trp Pro Pro Trp Thr Phe Gly Gln Gly 
        355                 360                 365             


Thr Lys Val Glu Ser Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile 
    370                 375                 380                 


Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val 
385                 390                 395                 400 


Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys 
                405                 410                 415     


Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu 
            420                 425                 430         


Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu 
        435                 440                 445             


Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr 
    450                 455                 460                 


His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu 
465                 470                 475                 480 


Cys Gly Ser Gly His His His His His His 
                485                 490 


<210>  73
<211>  500
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro-BDNF(-RGD)_(G4S)6GG_VL<IGF-1R>-Ck-His6

<400>  73

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
                245                 250                 255     


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
            260                 265                 270         


Gly Ser Gly Gly Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser 
        275                 280                 285             


Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser 
    290                 295                 300                 


Val Ser Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro 
305                 310                 315                 320 


Arg Leu Leu Ile Tyr Asp Ala Ser Lys Arg Ala Thr Gly Ile Pro Ala 
                325                 330                 335     


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
            340                 345                 350         


Ser Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Arg Ser 
        355                 360                 365             


Lys Trp Pro Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ser Lys 
    370                 375                 380                 


Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
385                 390                 395                 400 


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
                405                 410                 415     


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
            420                 425                 430         


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
        435                 440                 445             


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
    450                 455                 460                 


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
465                 470                 475                 480 


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys Gly Ser Gly His His 
                485                 490                 495     


His His His His 
            500 


<210>  74
<211>  481
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro-BDNF(-RGD)_(G4S)2GG_VH<IGF-1R>-CH1-His6

<400>  74

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
                245                 250                 255     


Gln Val Glu Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
            260                 265                 270         


Ser Gln Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
        275                 280                 285             


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
    290                 295                 300                 


Ala Ile Ile Trp Phe Asp Gly Ser Ser Thr Tyr Tyr Ala Asp Ser Val 
305                 310                 315                 320 


Arg Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
                325                 330                 335     


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Phe Cys 
            340                 345                 350         


Ala Arg Glu Leu Gly Arg Arg Tyr Phe Asp Leu Trp Gly Arg Gly Thr 
        355                 360                 365             


Leu Val Ser Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
    370                 375                 380                 


Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly 
385                 390                 395                 400 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
                405                 410                 415     


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
            420                 425                 430         


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
        435                 440                 445             


Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser 
    450                 455                 460                 


Asn Thr Lys Val Asp Lys Lys Val Gly Ser Gly His His His His His 
465                 470                 475                 480 


His 
    


<210>  75
<211>  491
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro-BDNF(-RGD)_(G4S)4GG_VH<IGF-1R>-CH1-His6

<400>  75

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
                245                 250                 255     


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gln Val Glu Leu Val Glu 
            260                 265                 270         


Ser Gly Gly Gly Val Val Gln Pro Gly Arg Ser Gln Arg Leu Ser Cys 
        275                 280                 285             


Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr Gly Met His Trp Val Arg 
    290                 295                 300                 


Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Ile Ile Trp Phe Asp 
305                 310                 315                 320 


Gly Ser Ser Thr Tyr Tyr Ala Asp Ser Val Arg Gly Arg Phe Thr Ile 
                325                 330                 335     


Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu 
            340                 345                 350         


Arg Ala Glu Asp Thr Ala Val Tyr Phe Cys Ala Arg Glu Leu Gly Arg 
        355                 360                 365             


Arg Tyr Phe Asp Leu Trp Gly Arg Gly Thr Leu Val Ser Val Ser Ser 
    370                 375                 380                 


Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
385                 390                 395                 400 


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
                405                 410                 415     


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
            420                 425                 430         


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
        435                 440                 445             


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
    450                 455                 460                 


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
465                 470                 475                 480 


Lys Val Gly Ser Gly His His His His His His 
                485                 490     


<210>  76
<211>  501
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro-BDNF(-RGD)_(G4S)6GG_VH<IGF-1R>-CH1-His6

<400>  76

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
                245                 250                 255     


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
            260                 265                 270         


Gly Ser Gly Gly Gln Val Glu Leu Val Glu Ser Gly Gly Gly Val Val 
        275                 280                 285             


Gln Pro Gly Arg Ser Gln Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr 
    290                 295                 300                 


Phe Ser Ser Tyr Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly 
305                 310                 315                 320 


Leu Glu Trp Val Ala Ile Ile Trp Phe Asp Gly Ser Ser Thr Tyr Tyr 
                325                 330                 335     


Ala Asp Ser Val Arg Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys 
            340                 345                 350         


Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
        355                 360                 365             


Val Tyr Phe Cys Ala Arg Glu Leu Gly Arg Arg Tyr Phe Asp Leu Trp 
    370                 375                 380                 


Gly Arg Gly Thr Leu Val Ser Val Ser Ser Ala Ser Thr Lys Gly Pro 
385                 390                 395                 400 


Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr 
                405                 410                 415     


Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr 
            420                 425                 430         


Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro 
        435                 440                 445             


Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr 
    450                 455                 460                 


Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn 
465                 470                 475                 480 


His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Gly Ser Gly His 
                485                 490                 495     


His His His His His 
            500     


<210>  77
<211>  493
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro-BDNF-(G3D)4-G3S-VH<IGF-1R>-CH1

<400>  77

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Gly Gly Asp Gly Gly Gly Asp Gly Gly Gly Asp 
                245                 250                 255     


Gly Gly Gly Asp Gly Gly Gly Asp Gly Gly Gly Ser Gln Val Glu Leu 
            260                 265                 270         


Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg Ser Gln Arg Leu 
        275                 280                 285             


Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr Gly Met His Trp 
    290                 295                 300                 


Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Ile Ile Trp 
305                 310                 315                 320 


Phe Asp Gly Ser Ser Thr Tyr Tyr Ala Asp Ser Val Arg Gly Arg Phe 
                325                 330                 335     


Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn 
            340                 345                 350         


Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Phe Cys Ala Arg Glu Leu 
        355                 360                 365             


Gly Arg Arg Tyr Phe Asp Leu Trp Gly Arg Gly Thr Leu Val Ser Val 
    370                 375                 380                 


Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser 
385                 390                 395                 400 


Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys 
                405                 410                 415     


Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu 
            420                 425                 430         


Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu 
        435                 440                 445             


Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr 
    450                 455                 460                 


Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val 
465                 470                 475                 480 


Asp Lys Lys Val Gly Ser Gly His His His His His His 
                485                 490             


<210>  78
<211>  513
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro-BDNF-(G4S)3-VL<IGF-1R>-(G4S)4-VH<IGF-1R>-His6

<400>  78

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
                245                 250                 255     


Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu 
            260                 265                 270         


Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val 
        275                 280                 285             


Ser Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg 
    290                 295                 300                 


Leu Leu Ile Tyr Asp Ala Ser Lys Arg Ala Thr Gly Ile Pro Ala Arg 
305                 310                 315                 320 


Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser 
                325                 330                 335     


Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Arg Ser Lys 
            340                 345                 350         


Trp Pro Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ser Lys Gly 
        355                 360                 365             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    370                 375                 380                 


Gly Gly Ser Gly Gly Gln Val Glu Leu Val Glu Ser Gly Gly Gly Val 
385                 390                 395                 400 


Val Gln Pro Gly Arg Ser Gln Arg Leu Ser Cys Ala Ala Ser Gly Phe 
                405                 410                 415     


Thr Phe Ser Ser Tyr Gly Met His Trp Val Arg Gln Ala Pro Gly Lys 
            420                 425                 430         


Gly Leu Glu Trp Val Ala Ile Ile Trp Phe Asp Gly Ser Ser Thr Tyr 
        435                 440                 445             


Tyr Ala Asp Ser Val Arg Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser 
    450                 455                 460                 


Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr 
465                 470                 475                 480 


Ala Val Tyr Phe Cys Ala Arg Glu Leu Gly Arg Arg Tyr Phe Asp Leu 
                485                 490                 495     


Trp Gly Arg Gly Thr Leu Val Ser Val Ser Ser His His His His His 
            500                 505                 510         


His 
    


<210>  79
<211>  517
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pro(BDNF) - IgA-Site - mBDNF - GSG - 2N - (G3D)4 - G3S - VH (8D3)
       - CH1 - GSG - His6

<400>  79

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Gly Ser Val Val 
        115                 120                 125             


Ala Pro Pro Ala Pro His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser 
    130                 135                 140                 


Val Cys Asp Ser Ile Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr 
145                 150                 155                 160 


Ala Val Asp Met Ser Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro 
                165                 170                 175     


Val Ser Lys Gly Gln Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn 
            180                 185                 190         


Pro Met Gly Tyr Thr Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His 
        195                 200                 205             


Trp Asn Ser Gln Cys Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr 
    210                 215                 220                 


Met Asp Ser Lys Lys Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr 
225                 230                 235                 240 


Ser Cys Val Cys Thr Leu Thr Ile Lys Gly Ser Gly Asn Gly Thr Glu 
                245                 250                 255     


Gly Pro Asn Phe Tyr Val Pro Phe Ser Asn Ala Thr Gly Val Val Arg 
            260                 265                 270         


Gly Gly Gly Asp Gly Gly Gly Asp Gly Gly Gly Asp Gly Gly Gly Asp 
        275                 280                 285             


Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val 
    290                 295                 300                 


Gln Pro Gly Asn Ser Leu Thr Leu Ser Cys Val Ala Ser Gly Phe Thr 
305                 310                 315                 320 


Phe Ser Asn Tyr Gly Met His Trp Ile Arg Gln Ala Pro Lys Lys Gly 
                325                 330                 335     


Leu Glu Trp Ile Ala Met Ile Tyr Tyr Asp Ser Ser Lys Met Asn Tyr 
            340                 345                 350         


Ala Asp Thr Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys 
        355                 360                 365             


Asn Thr Leu Tyr Leu Glu Met Asn Ser Leu Arg Ser Glu Asp Thr Ala 
    370                 375                 380                 


Met Tyr Tyr Cys Ala Val Pro Thr Ser His Tyr Val Val Asp Val Trp 
385                 390                 395                 400 


Gly Gln Gly Val Ser Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro 
                405                 410                 415     


Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr 
            420                 425                 430         


Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr 
        435                 440                 445             


Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro 
    450                 455                 460                 


Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr 
465                 470                 475                 480 


Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn 
                485                 490                 495     


His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Gly Ser Gly His 
            500                 505                 510         


His His His His His 
        515         


<210>  80
<211>  233
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL_8D3_chim. Ab rat-anti-murTfR VL _ hum const.

<400>  80

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Asp Ile Gln Met Thr Gln Ser Pro Ala Ser Leu Ser Ala 
            20                  25                  30          


Ser Leu Glu Glu Ile Val Thr Ile Thr Cys Gln Ala Ser Gln Asp Ile 
        35                  40                  45              


Gly Asn Trp Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ser Pro Gln 
    50                  55                  60                  


Leu Leu Ile Tyr Gly Ala Thr Ser Leu Ala Asp Gly Val Pro Ser Arg 
65                  70                  75                  80  


Phe Ser Gly Ser Arg Ser Gly Thr Gln Phe Ser Leu Lys Ile Ser Arg 
                85                  90                  95      


Val Gln Val Glu Asp Ile Gly Ile Tyr Tyr Cys Leu Gln Ala Tyr Asn 
            100                 105                 110         


Thr Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Leu Lys Arg Thr 
        115                 120                 125             


Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu 
    130                 135                 140                 


Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro 
145                 150                 155                 160 


Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly 
                165                 170                 175     


Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr 
            180                 185                 190         


Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His 
        195                 200                 205             


Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val 
    210                 215                 220                 


Thr Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230             


<210>  81
<211>  517
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro(IgA)-BDNF(-RGR; 
       R81N)_opsin(L)-(G3D)4-G3S_VH<TfR>8D3-CH1-His6

<400>  81

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Gly Ser Val Val 
        115                 120                 125             


Ala Pro Pro Ala Pro His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser 
    130                 135                 140                 


Val Cys Asp Ser Ile Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr 
145                 150                 155                 160 


Ala Val Asp Met Ser Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro 
                165                 170                 175     


Val Ser Lys Gly Gln Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn 
            180                 185                 190         


Pro Met Gly Tyr Thr Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His 
        195                 200                 205             


Trp Asn Ser Gln Cys Asn Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr 
    210                 215                 220                 


Met Asp Ser Lys Lys Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr 
225                 230                 235                 240 


Ser Cys Val Cys Thr Leu Thr Ile Lys Gly Ser Gly Asn Gly Thr Glu 
                245                 250                 255     


Gly Pro Asn Phe Tyr Val Pro Phe Ser Asn Ala Thr Gly Val Val Arg 
            260                 265                 270         


Gly Gly Gly Asp Gly Gly Gly Asp Gly Gly Gly Asp Gly Gly Gly Asp 
        275                 280                 285             


Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val 
    290                 295                 300                 


Gln Pro Gly Asn Ser Leu Thr Leu Ser Cys Val Ala Ser Gly Phe Thr 
305                 310                 315                 320 


Phe Ser Asn Tyr Gly Met His Trp Ile Arg Gln Ala Pro Lys Lys Gly 
                325                 330                 335     


Leu Glu Trp Ile Ala Met Ile Tyr Tyr Asp Ser Ser Lys Met Asn Tyr 
            340                 345                 350         


Ala Asp Thr Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys 
        355                 360                 365             


Asn Thr Leu Tyr Leu Glu Met Asn Ser Leu Arg Ser Glu Asp Thr Ala 
    370                 375                 380                 


Met Tyr Tyr Cys Ala Val Pro Thr Ser His Tyr Val Val Asp Val Trp 
385                 390                 395                 400 


Gly Gln Gly Val Ser Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro 
                405                 410                 415     


Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr 
            420                 425                 430         


Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr 
        435                 440                 445             


Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro 
    450                 455                 460                 


Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr 
465                 470                 475                 480 


Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn 
                485                 490                 495     


His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Gly Ser Gly His 
            500                 505                 510         


His His His His His 
        515         


<210>  82
<211>  278
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro-BDNF-IgA-mBDNF(R81N)-GSG-2N-His6

<400>  82

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Gly Ser Val Val 
        115                 120                 125             


Ala Pro Pro Ala Pro His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser 
    130                 135                 140                 


Val Cys Asp Ser Ile Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr 
145                 150                 155                 160 


Ala Val Asp Met Ser Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro 
                165                 170                 175     


Val Ser Lys Gly Gln Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn 
            180                 185                 190         


Pro Met Gly Tyr Thr Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His 
        195                 200                 205             


Trp Asn Ser Gln Cys Asn Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr 
    210                 215                 220                 


Met Asp Ser Lys Lys Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr 
225                 230                 235                 240 


Ser Cys Val Cys Thr Leu Thr Ile Lys Gly Ser Gly Asn Gly Thr Glu 
                245                 250                 255     


Gly Pro Asn Phe Tyr Val Pro Phe Ser Asn Ala Thr Gly Val Val Arg 
            260                 265                 270         


His His His His His His 
        275             


<210>  83
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  proBDNF (deltaRGR)-GSG-T7-His6

<400>  83

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  84
<211>  494
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro(IgA)-BDNF(-RGR)_(G3D)4-G3S_VH<IGF-1R>-CH1-His6

<400>  84

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Gly Ser Val Val 
        115                 120                 125             


Ala Pro Pro Ala Pro His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser 
    130                 135                 140                 


Val Cys Asp Ser Ile Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr 
145                 150                 155                 160 


Ala Val Asp Met Ser Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro 
                165                 170                 175     


Val Ser Lys Gly Gln Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn 
            180                 185                 190         


Pro Met Gly Tyr Thr Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His 
        195                 200                 205             


Trp Asn Ser Gln Cys Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr 
    210                 215                 220                 


Met Asp Ser Lys Lys Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr 
225                 230                 235                 240 


Ser Cys Val Cys Thr Leu Thr Ile Lys Gly Gly Gly Asp Gly Gly Gly 
                245                 250                 255     


Asp Gly Gly Gly Asp Gly Gly Gly Asp Gly Gly Gly Ser Gln Val Glu 
            260                 265                 270         


Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg Ser Gln Arg 
        275                 280                 285             


Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr Gly Met His 
    290                 295                 300                 


Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Ile Ile 
305                 310                 315                 320 


Trp Phe Asp Gly Ser Ser Thr Tyr Tyr Ala Asp Ser Val Arg Gly Arg 
                325                 330                 335     


Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met 
            340                 345                 350         


Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Phe Cys Ala Arg Glu 
        355                 360                 365             


Leu Gly Arg Arg Tyr Phe Asp Leu Trp Gly Arg Gly Thr Leu Val Ser 
    370                 375                 380                 


Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro 
385                 390                 395                 400 


Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val 
                405                 410                 415     


Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala 
            420                 425                 430         


Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly 
        435                 440                 445             


Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly 
    450                 455                 460                 


Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys 
465                 470                 475                 480 


Val Asp Lys Lys Val Gly Ser Gly His His His His His His 
                485                 490                 


<210>  85
<211>  499
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro(IgA)-BDNF(-RGR)_(G3D)4-G3S_VH<IGF-1R>-CH1-EPKSC-His6

<400>  85

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Gly Ser Val Val 
        115                 120                 125             


Ala Pro Pro Ala Pro His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser 
    130                 135                 140                 


Val Cys Asp Ser Ile Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr 
145                 150                 155                 160 


Ala Val Asp Met Ser Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro 
                165                 170                 175     


Val Ser Lys Gly Gln Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn 
            180                 185                 190         


Pro Met Gly Tyr Thr Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His 
        195                 200                 205             


Trp Asn Ser Gln Cys Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr 
    210                 215                 220                 


Met Asp Ser Lys Lys Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr 
225                 230                 235                 240 


Ser Cys Val Cys Thr Leu Thr Ile Lys Gly Gly Gly Asp Gly Gly Gly 
                245                 250                 255     


Asp Gly Gly Gly Asp Gly Gly Gly Asp Gly Gly Gly Ser Gln Val Glu 
            260                 265                 270         


Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg Ser Gln Arg 
        275                 280                 285             


Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr Gly Met His 
    290                 295                 300                 


Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Ile Ile 
305                 310                 315                 320 


Trp Phe Asp Gly Ser Ser Thr Tyr Tyr Ala Asp Ser Val Arg Gly Arg 
                325                 330                 335     


Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met 
            340                 345                 350         


Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Phe Cys Ala Arg Glu 
        355                 360                 365             


Leu Gly Arg Arg Tyr Phe Asp Leu Trp Gly Arg Gly Thr Leu Val Ser 
    370                 375                 380                 


Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro 
385                 390                 395                 400 


Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val 
                405                 410                 415     


Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala 
            420                 425                 430         


Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly 
        435                 440                 445             


Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly 
    450                 455                 460                 


Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys 
465                 470                 475                 480 


Val Asp Lys Lys Val Glu Pro Lys Ser Cys Gly Ser Gly His His His 
                485                 490                 495     


His His His 
            


<210>  86
<211>  517
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro(IgA)-BDNF(-RGR; 
       R81N)_opsin(L)-(G3D)4-G3S_VH<IGF-1R>-CH1-His6

<400>  86

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Gly Ser Val Val 
        115                 120                 125             


Ala Pro Pro Ala Pro His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser 
    130                 135                 140                 


Val Cys Asp Ser Ile Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr 
145                 150                 155                 160 


Ala Val Asp Met Ser Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro 
                165                 170                 175     


Val Ser Lys Gly Gln Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn 
            180                 185                 190         


Pro Met Gly Tyr Thr Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His 
        195                 200                 205             


Trp Asn Ser Gln Cys Asn Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr 
    210                 215                 220                 


Met Asp Ser Lys Lys Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr 
225                 230                 235                 240 


Ser Cys Val Cys Thr Leu Thr Ile Lys Gly Ser Gly Asn Gly Thr Glu 
                245                 250                 255     


Gly Pro Asn Phe Tyr Val Pro Phe Ser Asn Ala Thr Gly Val Val Arg 
            260                 265                 270         


Gly Gly Gly Asp Gly Gly Gly Asp Gly Gly Gly Asp Gly Gly Gly Asp 
        275                 280                 285             


Gly Gly Gly Ser Gln Val Glu Leu Val Glu Ser Gly Gly Gly Val Val 
    290                 295                 300                 


Gln Pro Gly Arg Ser Gln Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr 
305                 310                 315                 320 


Phe Ser Ser Tyr Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly 
                325                 330                 335     


Leu Glu Trp Val Ala Ile Ile Trp Phe Asp Gly Ser Ser Thr Tyr Tyr 
            340                 345                 350         


Ala Asp Ser Val Arg Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys 
        355                 360                 365             


Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
    370                 375                 380                 


Val Tyr Phe Cys Ala Arg Glu Leu Gly Arg Arg Tyr Phe Asp Leu Trp 
385                 390                 395                 400 


Gly Arg Gly Thr Leu Val Ser Val Ser Ser Ala Ser Thr Lys Gly Pro 
                405                 410                 415     


Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr 
            420                 425                 430         


Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr 
        435                 440                 445             


Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro 
    450                 455                 460                 


Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr 
465                 470                 475                 480 


Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn 
                485                 490                 495     


His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Gly Ser Gly His 
            500                 505                 510         


His His His His His 
        515         


<210>  87
<211>  516
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro(IgA)-BDNF(-RGR; R81N)_opsin(L)-(G2D)5-G2SG 
       _VH<IGF-1R>-CH1-His6

<400>  87

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Gly Ser Val Val 
        115                 120                 125             


Ala Pro Pro Ala Pro His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser 
    130                 135                 140                 


Val Cys Asp Ser Ile Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr 
145                 150                 155                 160 


Ala Val Asp Met Ser Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro 
                165                 170                 175     


Val Ser Lys Gly Gln Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn 
            180                 185                 190         


Pro Met Gly Tyr Thr Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His 
        195                 200                 205             


Trp Asn Ser Gln Cys Asn Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr 
    210                 215                 220                 


Met Asp Ser Lys Lys Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr 
225                 230                 235                 240 


Ser Cys Val Cys Thr Leu Thr Ile Lys Gly Ser Gly Asn Gly Thr Glu 
                245                 250                 255     


Gly Pro Asn Phe Tyr Val Pro Phe Ser Asn Ala Thr Gly Val Val Arg 
            260                 265                 270         


Gly Gly Asp Gly Gly Asp Gly Gly Asp Gly Gly Asp Gly Gly Asp Gly 
        275                 280                 285             


Gly Ser Gly Gln Val Glu Leu Val Glu Ser Gly Gly Gly Val Val Gln 
    290                 295                 300                 


Pro Gly Arg Ser Gln Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
305                 310                 315                 320 


Ser Ser Tyr Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
                325                 330                 335     


Glu Trp Val Ala Ile Ile Trp Phe Asp Gly Ser Ser Thr Tyr Tyr Ala 
            340                 345                 350         


Asp Ser Val Arg Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn 
        355                 360                 365             


Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
    370                 375                 380                 


Tyr Phe Cys Ala Arg Glu Leu Gly Arg Arg Tyr Phe Asp Leu Trp Gly 
385                 390                 395                 400 


Arg Gly Thr Leu Val Ser Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 
                405                 410                 415     


Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 
            420                 425                 430         


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
        435                 440                 445             


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
    450                 455                 460                 


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
465                 470                 475                 480 


Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His 
                485                 490                 495     


Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Gly Ser Gly His His 
            500                 505                 510         


His His His His 
        515     


<210>  88
<211>  493
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro-BDNF-(G3D)4-G3S-VH<IGF-1R>-CH1

<400>  88

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Gly Gly Asp Gly Gly Gly Asp Gly Gly Gly Asp 
                245                 250                 255     


Gly Gly Gly Asp Gly Gly Gly Asp Gly Gly Gly Ser Gln Val Glu Leu 
            260                 265                 270         


Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg Ser Gln Arg Leu 
        275                 280                 285             


Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr Gly Met His Trp 
    290                 295                 300                 


Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Ile Ile Trp 
305                 310                 315                 320 


Phe Asp Gly Ser Ser Thr Tyr Tyr Ala Asp Ser Val Arg Gly Arg Phe 
                325                 330                 335     


Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn 
            340                 345                 350         


Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Phe Cys Ala Arg Glu Leu 
        355                 360                 365             


Gly Arg Arg Tyr Phe Asp Leu Trp Gly Arg Gly Thr Leu Val Ser Val 
    370                 375                 380                 


Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser 
385                 390                 395                 400 


Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys 
                405                 410                 415     


Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu 
            420                 425                 430         


Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu 
        435                 440                 445             


Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr 
    450                 455                 460                 


Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val 
465                 470                 475                 480 


Asp Lys Lys Val Gly Ser Gly His His His His His His 
                485                 490             


<210>  89
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pre-pro-BDNF(-RGR; R81N)-T7-His6

<400>  89

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Asn Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  90
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  90

Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  


<210>  91
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pre-pro-BDNF(-RGR; Q79S)-T7-His6

<400>  91

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Ser Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  92
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pre-pro-BDNF(-RGR; W19N)-T7-His6

<400>  92

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Asn Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  93
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pre-pro-BDNF(-RGR; K25N)-T7-His6

<400>  93

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Asn Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  94
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pre-pro-BDNF(-RGR; D30N)-T7-His6

<400>  94

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asn Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  95
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pre-pro-BDNF(-RGR; G33N)-T7-His6

<400>  95

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Asn Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  96
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pre-pro-BDNF(-RGR; T35N)-T7-His6

<400>  96

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Asn Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  97
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pre-pro-BDNF(-RGR; P43N)-T7-His6

<400>  97

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Asn Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  98
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pre-pro-BDNF(-RGR; P43N, V42G)-T7-His6

<400>  98

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Gly Asn Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  99
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pre-pro-BDNF(-RGR; M61S, P60G)-T7-His6

<400>  99

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Gly Ser Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  100
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pre-pro-BDNF(-RGR; G62N, Y63G)-T7-His6

<400>  100

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Asn Gly Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  101
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pre-pro-BDNF(-RGR; Q79T)-T7-His6

<400>  101

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Thr Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  102
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pre-pro-BDNF(-RGR; W76N)-T7-His6

<400>  102

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Asn Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  103
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pre-pro-BDNF(-RGR; M92N)-T7-His6

<400>  103

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Asn Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  104
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pre-pro-BDNF(-RGR; D106N)-T7-His6

<400>  104

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asn Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  105
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pre-pro-BDNF(-RGR; T112N)-T7-His6

<400>  105

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Gln Cys 
        195                 200                 205             


Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Asn 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  106
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro-BDNF(-RGR; Q79T, R81N)-T7-His6

<400>  106

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Arg Val Arg Arg 
        115                 120                 125             


His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser Val Cys Asp Ser Ile 
    130                 135                 140                 


Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr Ala Val Asp Met Ser 
145                 150                 155                 160 


Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro Val Ser Lys Gly Gln 
                165                 170                 175     


Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn Pro Met Gly Tyr Thr 
            180                 185                 190         


Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His Trp Asn Ser Thr Cys 
        195                 200                 205             


Asn Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr Met Asp Ser Lys Lys 
    210                 215                 220                 


Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr Ser Cys Val Cys Thr 
225                 230                 235                 240 


Leu Thr Ile Lys Gly Ser Gly Met Ala Ser Met Thr Gly Gly Gln Gln 
                245                 250                 255     


Met Gly His His His His His His 
            260                 


<210>  107
<211>  499
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro(IgA)-BDNF(-RGR)_(G3D)4-G3S_VH<TfR>8D3-CH1-EPKSC-His6

<400>  107

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Gly Ser Val Val 
        115                 120                 125             


Ala Pro Pro Ala Pro His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser 
    130                 135                 140                 


Val Cys Asp Ser Ile Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr 
145                 150                 155                 160 


Ala Val Asp Met Ser Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro 
                165                 170                 175     


Val Ser Lys Gly Gln Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn 
            180                 185                 190         


Pro Met Gly Tyr Thr Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His 
        195                 200                 205             


Trp Asn Ser Gln Cys Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr 
    210                 215                 220                 


Met Asp Ser Lys Lys Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr 
225                 230                 235                 240 


Ser Cys Val Cys Thr Leu Thr Ile Lys Gly Gly Gly Asp Gly Gly Gly 
                245                 250                 255     


Asp Gly Gly Gly Asp Gly Gly Gly Asp Gly Gly Gly Ser Glu Val Gln 
            260                 265                 270         


Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Asn Ser Leu Thr 
        275                 280                 285             


Leu Ser Cys Val Ala Ser Gly Phe Thr Phe Ser Asn Tyr Gly Met His 
    290                 295                 300                 


Trp Ile Arg Gln Ala Pro Lys Lys Gly Leu Glu Trp Ile Ala Met Ile 
305                 310                 315                 320 


Tyr Tyr Asp Ser Ser Lys Met Asn Tyr Ala Asp Thr Val Lys Gly Arg 
                325                 330                 335     


Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Glu Met 
            340                 345                 350         


Asn Ser Leu Arg Ser Glu Asp Thr Ala Met Tyr Tyr Cys Ala Val Pro 
        355                 360                 365             


Thr Ser His Tyr Val Val Asp Val Trp Gly Gln Gly Val Ser Val Thr 
    370                 375                 380                 


Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro 
385                 390                 395                 400 


Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val 
                405                 410                 415     


Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala 
            420                 425                 430         


Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly 
        435                 440                 445             


Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly 
    450                 455                 460                 


Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys 
465                 470                 475                 480 


Val Asp Lys Lys Val Glu Pro Lys Ser Cys Gly Ser Gly His His His 
                485                 490                 495     


His His His 
            


<210>  108
<211>  494
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro(IgA)-BDNF(-RGR)_(G3D)4-G3S_VH<TfR>8D3-CH1-His6

<400>  108

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Gly Ser Val Val 
        115                 120                 125             


Ala Pro Pro Ala Pro His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser 
    130                 135                 140                 


Val Cys Asp Ser Ile Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr 
145                 150                 155                 160 


Ala Val Asp Met Ser Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro 
                165                 170                 175     


Val Ser Lys Gly Gln Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn 
            180                 185                 190         


Pro Met Gly Tyr Thr Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His 
        195                 200                 205             


Trp Asn Ser Gln Cys Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr 
    210                 215                 220                 


Met Asp Ser Lys Lys Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr 
225                 230                 235                 240 


Ser Cys Val Cys Thr Leu Thr Ile Lys Gly Gly Gly Asp Gly Gly Gly 
                245                 250                 255     


Asp Gly Gly Gly Asp Gly Gly Gly Asp Gly Gly Gly Ser Glu Val Gln 
            260                 265                 270         


Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Asn Ser Leu Thr 
        275                 280                 285             


Leu Ser Cys Val Ala Ser Gly Phe Thr Phe Ser Asn Tyr Gly Met His 
    290                 295                 300                 


Trp Ile Arg Gln Ala Pro Lys Lys Gly Leu Glu Trp Ile Ala Met Ile 
305                 310                 315                 320 


Tyr Tyr Asp Ser Ser Lys Met Asn Tyr Ala Asp Thr Val Lys Gly Arg 
                325                 330                 335     


Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Glu Met 
            340                 345                 350         


Asn Ser Leu Arg Ser Glu Asp Thr Ala Met Tyr Tyr Cys Ala Val Pro 
        355                 360                 365             


Thr Ser His Tyr Val Val Asp Val Trp Gly Gln Gly Val Ser Val Thr 
    370                 375                 380                 


Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro 
385                 390                 395                 400 


Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val 
                405                 410                 415     


Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala 
            420                 425                 430         


Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly 
        435                 440                 445             


Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly 
    450                 455                 460                 


Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys 
465                 470                 475                 480 


Val Asp Lys Lys Val Gly Ser Gly His His His His His His 
                485                 490                 


<210>  109
<211>  485
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pre-pro(IgA)-BDNF(-RGR)_(G3D)4-G3S_VH<TfR>8D3-CH1

<400>  109

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Lys Glu Ala Asn Ile Arg Gly Gln Gly Gly Leu 
            20                  25                  30          


Ala Tyr Pro Gly Val Arg Thr His Gly Thr Leu Glu Ser Val Asn Gly 
        35                  40                  45              


Pro Lys Ala Gly Ser Arg Gly Leu Thr Ser Leu Ala Asp Thr Phe Glu 
    50                  55                  60                  


His Val Ile Glu Glu Leu Leu Asp Glu Asp Gln Lys Val Arg Pro Asn 
65                  70                  75                  80  


Glu Glu Asn Asn Lys Asp Ala Asp Leu Tyr Thr Ser Arg Val Met Leu 
                85                  90                  95      


Ser Ser Gln Val Pro Leu Glu Pro Pro Leu Leu Phe Leu Leu Glu Glu 
            100                 105                 110         


Tyr Lys Asn Tyr Leu Asp Ala Ala Asn Met Ser Met Gly Ser Val Val 
        115                 120                 125             


Ala Pro Pro Ala Pro His Ser Asp Pro Ala Arg Arg Gly Glu Leu Ser 
    130                 135                 140                 


Val Cys Asp Ser Ile Ser Glu Trp Val Thr Ala Ala Asp Lys Lys Thr 
145                 150                 155                 160 


Ala Val Asp Met Ser Gly Gly Thr Val Thr Val Leu Glu Lys Val Pro 
                165                 170                 175     


Val Ser Lys Gly Gln Leu Lys Gln Tyr Phe Tyr Glu Thr Lys Cys Asn 
            180                 185                 190         


Pro Met Gly Tyr Thr Lys Glu Gly Cys Arg Gly Ile Asp Lys Arg His 
        195                 200                 205             


Trp Asn Ser Gln Cys Arg Thr Thr Gln Ser Tyr Val Arg Ala Leu Thr 
    210                 215                 220                 


Met Asp Ser Lys Lys Arg Ile Gly Trp Arg Phe Ile Arg Ile Asp Thr 
225                 230                 235                 240 


Ser Cys Val Cys Thr Leu Thr Ile Lys Gly Gly Gly Asp Gly Gly Gly 
                245                 250                 255     


Asp Gly Gly Gly Asp Gly Gly Gly Asp Gly Gly Gly Ser Glu Val Gln 
            260                 265                 270         


Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Asn Ser Leu Thr 
        275                 280                 285             


Leu Ser Cys Val Ala Ser Gly Phe Thr Phe Ser Asn Tyr Gly Met His 
    290                 295                 300                 


Trp Ile Arg Gln Ala Pro Lys Lys Gly Leu Glu Trp Ile Ala Met Ile 
305                 310                 315                 320 


Tyr Tyr Asp Ser Ser Lys Met Asn Tyr Ala Asp Thr Val Lys Gly Arg 
                325                 330                 335     


Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Glu Met 
            340                 345                 350         


Asn Ser Leu Arg Ser Glu Asp Thr Ala Met Tyr Tyr Cys Ala Val Pro 
        355                 360                 365             


Thr Ser His Tyr Val Val Asp Val Trp Gly Gln Gly Val Ser Val Thr 
    370                 375                 380                 


Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro 
385                 390                 395                 400 


Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val 
                405                 410                 415     


Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala 
            420                 425                 430         


Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly 
        435                 440                 445             


Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly 
    450                 455                 460                 


Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys 
465                 470                 475                 480 


Val Asp Lys Lys Val 
                485 


