                         SEQUENCE LISTING

<110>  Yeda Research and Development Co. Ltd.
       BAR-EVEN Arren
       MILO, Ron
       NOOR, Elad
       ZELCBUCH, Lior
 
<120>  USE OF ENZYMES WHICH CATALYZE PYRUVATE SYNTHESIS FROM FORMATE AND
        ACETYL-COA AND BACTERIA EXPRESSING SAME

<130>  60665

<150>  US 61/913,940
<151>  2013-12-10

<160>  2     

<170>  PatentIn version 3.5

<210>  1
<211>  760
<212>  PRT
<213>  Escherichia coli

<400>  1

Met Ser Glu Leu Asn Glu Lys Leu Ala Thr Ala Trp Glu Gly Phe Thr 
1               5                   10                  15      


Lys Gly Asp Trp Gln Asn Glu Val Asn Val Arg Asp Phe Ile Gln Lys 
            20                  25                  30          


Asn Tyr Thr Pro Tyr Glu Gly Asp Glu Ser Phe Leu Ala Gly Ala Thr 
        35                  40                  45              


Glu Ala Thr Thr Thr Leu Trp Asp Lys Val Met Glu Gly Val Lys Leu 
    50                  55                  60                  


Glu Asn Arg Thr His Ala Pro Val Asp Phe Asp Thr Ala Val Ala Ser 
65                  70                  75                  80  


Thr Ile Thr Ser His Asp Ala Gly Tyr Ile Asn Lys Gln Leu Glu Lys 
                85                  90                  95      


Ile Val Gly Leu Gln Thr Glu Ala Pro Leu Lys Arg Ala Leu Ile Pro 
            100                 105                 110         


Phe Gly Gly Ile Lys Met Ile Glu Gly Ser Cys Lys Ala Tyr Asn Arg 
        115                 120                 125             


Glu Leu Asp Pro Met Ile Lys Lys Ile Phe Thr Glu Tyr Arg Lys Thr 
    130                 135                 140                 


His Asn Gln Gly Val Phe Asp Val Tyr Thr Pro Asp Ile Leu Arg Cys 
145                 150                 155                 160 


Arg Lys Ser Gly Val Leu Thr Gly Leu Pro Asp Ala Tyr Gly Arg Gly 
                165                 170                 175     


Arg Ile Ile Gly Asp Tyr Arg Arg Val Ala Leu Tyr Gly Ile Asp Tyr 
            180                 185                 190         


Leu Met Lys Asp Lys Leu Ala Gln Phe Thr Ser Leu Gln Ala Asp Leu 
        195                 200                 205             


Glu Asn Gly Val Asn Leu Glu Gln Thr Ile Arg Leu Arg Glu Glu Ile 
    210                 215                 220                 


Ala Glu Gln His Arg Ala Leu Gly Gln Met Lys Glu Met Ala Ala Lys 
225                 230                 235                 240 


Tyr Gly Tyr Asp Ile Ser Gly Pro Ala Thr Asn Ala Gln Glu Ala Ile 
                245                 250                 255     


Gln Trp Thr Tyr Phe Gly Tyr Leu Ala Ala Val Lys Ser Gln Asn Gly 
            260                 265                 270         


Ala Ala Met Ser Phe Gly Arg Thr Ser Thr Phe Leu Asp Val Tyr Ile 
        275                 280                 285             


Glu Arg Asp Leu Lys Ala Gly Lys Ile Thr Glu Gln Glu Ala Gln Glu 
    290                 295                 300                 


Met Val Asp His Leu Val Met Lys Leu Arg Met Val Arg Phe Leu Arg 
305                 310                 315                 320 


Thr Pro Glu Tyr Asp Glu Leu Phe Ser Gly Asp Pro Ile Trp Ala Thr 
                325                 330                 335     


Glu Ser Ile Gly Gly Met Gly Leu Asp Gly Arg Thr Leu Val Thr Lys 
            340                 345                 350         


Asn Ser Phe Arg Phe Leu Asn Thr Leu Tyr Thr Met Gly Pro Ser Pro 
        355                 360                 365             


Glu Pro Asn Met Thr Ile Leu Trp Ser Glu Lys Leu Pro Leu Asn Phe 
    370                 375                 380                 


Lys Lys Phe Ala Ala Lys Val Ser Ile Asp Thr Ser Ser Leu Gln Tyr 
385                 390                 395                 400 


Glu Asn Asp Asp Leu Met Arg Pro Asp Phe Asn Asn Asp Asp Tyr Ala 
                405                 410                 415     


Ile Ala Cys Cys Val Ser Pro Met Ile Val Gly Lys Gln Met Gln Phe 
            420                 425                 430         


Phe Gly Ala Arg Ala Asn Leu Ala Lys Thr Met Leu Tyr Ala Ile Asn 
        435                 440                 445             


Gly Gly Val Asp Glu Lys Leu Lys Met Gln Val Gly Pro Lys Ser Glu 
    450                 455                 460                 


Pro Ile Lys Gly Asp Val Leu Asn Tyr Asp Glu Val Met Glu Arg Met 
465                 470                 475                 480 


Asp His Phe Met Asp Trp Leu Ala Lys Gln Tyr Ile Thr Ala Leu Asn 
                485                 490                 495     


Ile Ile His Tyr Met His Asp Lys Tyr Ser Tyr Glu Ala Ser Leu Met 
            500                 505                 510         


Ala Leu His Asp Arg Asp Val Ile Arg Thr Met Ala Cys Gly Ile Ala 
        515                 520                 525             


Gly Leu Ser Val Ala Ala Asp Ser Leu Ser Ala Ile Lys Tyr Ala Lys 
    530                 535                 540                 


Val Lys Pro Ile Arg Asp Glu Asp Gly Leu Ala Ile Asp Phe Glu Ile 
545                 550                 555                 560 


Glu Gly Glu Tyr Pro Gln Phe Gly Asn Asn Asp Pro Arg Val Asp Asp 
                565                 570                 575     


Leu Ala Val Asp Leu Val Glu Arg Phe Met Lys Lys Ile Gln Lys Leu 
            580                 585                 590         


His Thr Tyr Arg Asp Ala Ile Pro Thr Gln Ser Val Leu Thr Ile Thr 
        595                 600                 605             


Ser Asn Val Val Tyr Gly Lys Lys Thr Gly Asn Thr Pro Asp Gly Arg 
    610                 615                 620                 


Arg Ala Gly Ala Pro Phe Gly Pro Gly Ala Asn Pro Met His Gly Arg 
625                 630                 635                 640 


Asp Gln Lys Gly Ala Val Ala Ser Leu Thr Ser Val Ala Lys Leu Pro 
                645                 650                 655     


Phe Ala Tyr Ala Lys Asp Gly Ile Ser Tyr Thr Phe Ser Ile Val Pro 
            660                 665                 670         


Asn Ala Leu Gly Lys Asp Asp Glu Val Arg Lys Thr Asn Leu Ala Gly 
        675                 680                 685             


Leu Met Asp Gly Tyr Phe His His Glu Ala Ser Ile Glu Gly Gly Gln 
    690                 695                 700                 


His Leu Asn Val Asn Val Met Asn Arg Glu Met Leu Leu Asp Ala Met 
705                 710                 715                 720 


Glu Asn Pro Glu Lys Tyr Pro Gln Leu Thr Ile Arg Val Ser Gly Tyr 
                725                 730                 735     


Ala Val Arg Phe Asn Ser Leu Thr Lys Glu Gln Gln Gln Asp Val Ile 
            740                 745                 750         


Thr Arg Thr Phe Thr Gln Ser Met 
        755                 760 


<210>  2
<211>  246
<212>  PRT
<213>  Escherichia coli

<400>  2

Met Ser Val Ile Gly Arg Ile His Ser Phe Glu Ser Cys Gly Thr Val 
1               5                   10                  15      


Asp Gly Pro Gly Ile Arg Phe Ile Thr Phe Phe Gln Gly Cys Leu Met 
            20                  25                  30          


Arg Cys Leu Tyr Cys His Asn Arg Asp Thr Trp Asp Thr His Gly Gly 
        35                  40                  45              


Lys Glu Val Thr Val Glu Asp Leu Met Lys Glu Val Val Thr Tyr Arg 
    50                  55                  60                  


His Phe Met Asn Ala Ser Gly Gly Gly Val Thr Ala Ser Gly Gly Glu 
65                  70                  75                  80  


Ala Ile Leu Gln Ala Glu Phe Val Arg Asp Trp Phe Arg Ala Cys Lys 
                85                  90                  95      


Lys Glu Gly Ile His Thr Cys Leu Asp Thr Asn Gly Phe Val Arg Arg 
            100                 105                 110         


Tyr Asp Pro Val Ile Asp Glu Leu Leu Glu Val Thr Asp Leu Val Met 
        115                 120                 125             


Leu Asp Leu Lys Gln Met Asn Asp Glu Ile His Gln Asn Leu Val Gly 
    130                 135                 140                 


Val Ser Asn His Arg Thr Leu Glu Phe Ala Lys Tyr Leu Ala Asn Lys 
145                 150                 155                 160 


Asn Val Lys Val Trp Ile Arg Tyr Val Val Val Pro Gly Trp Ser Asp 
                165                 170                 175     


Asp Asp Asp Ser Ala His Arg Leu Gly Glu Phe Thr Arg Asp Met Gly 
            180                 185                 190         


Asn Val Glu Lys Ile Glu Leu Leu Pro Tyr His Glu Leu Gly Lys His 
        195                 200                 205             


Lys Trp Val Ala Met Gly Glu Glu Tyr Lys Leu Asp Gly Val Lys Pro 
    210                 215                 220                 


Pro Lys Lys Glu Thr Met Glu Arg Val Lys Gly Ile Leu Glu Gln Tyr 
225                 230                 235                 240 


Gly His Lys Val Met Phe 
                245     


