                         SEQUENCE LISTING

<110>  DSM IP Assets B.V.
 
<120>  FUMARATE REDUCTASES

<130>  29929-WO-PCT

<150>  EP13196950.3 
<151>  2013-12-02

<160>  41    

<170>  PatentIn version 3.5

<210>  1
<211>  2626
<212>  DNA
<213>  Actinobacillus succinogenes

<400>  1
ttgcccatcg aacgtacaag tactcctctg ttctctcctt cctttgcttt gtgcgacacc       60

taactacata gtgtttaaag attacggata tttaacttac ttagaataat gccatttttt      120

tgagttataa taatcctacg ttagtgtgag cgggatttaa actgtgagga ccttaataca      180

ttcagacact tctgcggtat caccctactt attcccttcg agattatatc taggaaccca      240

tcaggttggt ggaagattac ccgttctaag acttttcagc ttcctctatt gatgttacac      300

ctggacaccc cttttctggc atccagtttt taatcttcag tggcatgtga gattctccga      360

aattaattaa agcaatcaca caattctctc ggataccacc tcggttgaaa ctgacaggtg      420

gtttgttacg catgctaatg caaaggagcc tatatacctt tggctcggct gctgtaacag      480

ggaatataaa gggcagcata atttaggagt ttagtgaact tgcaacattt actattttcc      540

cttcttacgt aaatattttt ctttttaatt ctaaatcaat ctttttcaat tttttgtttg      600

tattcttttc ttgcttaaat ctataactac aaaaaacaca tacataaact aaaaatgact      660

gatttgaaca aattggtcaa ggaattgaat gatttgggtt tgactgacgt caaggaaatt      720

gtctacaacc catcttacga acaattattc gaagaagaaa ccaagccagg tttggaaggt      780

ttcgacaagg gtactttgac cactttaggt gctgttgctg ttgacaccgg tattttcacc      840

ggtcgttctc caaaggacaa atacattgtt tgtgatgaaa ccaccaagga caccgtctgg      900

tggaactctg aagctgccaa gaacgataac aagccaatga ctcaagaaac ctggaaatct      960

ttgagagaat tggttgccaa gcaattgtct ggtaagagat tattcgttgt tgacgctttc     1020

tgtggtgctt ctgaaaagca cagaattggt gtcagaatgg tcactgaagt tgcttggcaa     1080

gctcatttcg tcaagaacat gttcatcaga ccaactgacg aagaattgaa gaacttcaag     1140

gctgacttca ccgttttgaa tggtgccaag tgtaccaacc caaactggaa ggaacaaggt     1200

ttgaactctg aaaactttgt tgctttcaac atcactgaag gtatccaatt gattggtggt     1260

acttggtacg gtggtgaaat gaagaagggt atgttctcca tgatgaacta tttcttgcca     1320

ttgaaaggtg ttgcttccat gcactgttct gccaatgtcg gtaaggatgg tgacgttgcc     1380

atcttcttcg gtctatccgg tactggtaag accactctat ccactgaccc aaagagacaa     1440

ttgattggtg atgacgaaca cggttgggac gaatctggtg tctttaactt tgaaggtggt     1500

tgttacgcca agaccatcaa cttatctcaa gaaaacgaac cagatatcta cggtgccatc     1560

cgtcgtgatg ctttgttgga aaacgttgtt gtcagagctg acggttctgt tgacttcgac     1620

gacggttcca agactgaaaa caccagagtt tcttacccaa tctaccacat tgacaacatt     1680

gtcagacctg tttccaaggc tggtcacgct accaaggtta tcttcttgac tgctgatgct     1740

ttcggtgtct tgccacctgt ttccaaattg actccagaac aaaccgaata ctacttcttg     1800

tccggtttca ctgccaaatt ggctggtact gaaagaggtg tcactgaacc aactccaact     1860

ttctctgctt gtttcggtgc tgctttctta tctttgcacc caatccaata cgctgatgtc     1920

ttggttgaaa gaatgaaggc ttctggtgct gaagcttact tggtcaacac cggttggaac     1980

ggtactggta agagaatctc catcaaggat accagaggta tcattgatgc tatcttggac     2040

ggttccattg aaaaggctga aatgggtgaa ttgccaatct tcaacttggc cattccaaag     2100

gctttgccag gtgttgaccc agccatctta gatccaagag acacctacgc tgacaaggct     2160

caatggcaag tcaaggctga agatttggct aacagattcg tcaagaactt tgtcaaatac     2220

actgctaacc cagaagctgc caaattggtt ggtgctggtc caaaggctta aaggagttaa     2280

aggcaaagtt ttcttttcta gagccgttcc cacaaataat tatacgtata tgcttctttt     2340

cgtttactat atatctatat ttacaagcct ttattcactg atgcaatttg tttccaaata     2400

cttttttgga gatctcataa ctagatatca tgatggcgca acttggcgct atcttaatta     2460

ctctggctgc caggcccgtg tagagggccg caagaccttc tgtacgccat atagtctcta     2520

agaacttgaa caagtttcta gacctattgc cgcctttcgg atcgctattg ttcctccgga     2580

tcgatgtaca caaccgactg cacccaaacg aacacaaatc ttagca                    2626


<210>  2
<211>  4552
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  2
cggatcgatg tacacaaccg actgcaccca aacgaacaca aatcttagca gtgcgggcca       60

gaaaaaggaa gtgtttccct ccttcttgaa ttgatgttac cctcataaag cacgtggcct      120

cttatcgaga aagaaattac cgtcgctcgt gatttgtttg caaaaagaac aaaactgaaa      180

aaacccagac acgctcgact tcctgtcttc ctattgattg cagcttccaa tttcgtcaca      240

caacaaggtc ctagcgacgg ctcacaggtt ttgtaacaag caatcgaagg ttctggaatg      300

gcgggaaagg gtttagtacc acatgctatg atgcccactg tgatctccag agcaaagttc      360

gttcgatcgt actgttactc tctctctttc aaacagaatt gtccgaatcg tgtgacaaca      420

acagcctgtt ctcacacact cttttcttct aaccaagggg gtggtttagt ttagtagaac      480

ctcgtgaaac ttacatttac atatatataa acttgcataa attggtcaat gcaagaaata      540

catatttggt cttttctaat tcgtagtttt tcaagttctt agatgctttc tttttctctt      600

ttttacagat catcaaggaa gtaattatct actttttaca acaaatataa aacaatgtcc      660

tcttccaaga tcttggctgg tttgagagac aacttttctt tgttgggtga aaagaacaag      720

attttggtcg ccaacagagg tgaaatccca atcagaattt tcagatctgc tcacgaattg      780

tctatgagaa ctatcgccat ctactctcac gaagatagat tatccatgca cagattgaag      840

gctgatgaag cctacgttat cggtgaagaa ggtcaataca ccccagtcgg tgcttacttg      900

gccatggacg aaatcatcga aattgccaag aagcacaagg tcgatttcat ccacccaggt      960

tacggtttct tgtctgaaaa ctctgaattt gctgacaagg ttgttaaggc tggtattacc     1020

tggattggtc caccagctga agtcattgaa tctgttggtg acaaggtttc tgccagacat     1080

ttggctgctc gtgccaacgt tccaactgtc ccaggtactc caggtcctat cgaaaccgtt     1140

caagaagctc tagatttcgt caatgaatac ggttacccag ttatcatcaa ggctgctttc     1200

ggtggtggtg gtcgtggtat gagagttgtc agagaaggtg acgatgtcgc tgatgctttc     1260

caaagagcca cttctgaagc tagaactgct ttcggtaacg gtacttgttt cgtcgaaaga     1320

ttcttggaca agccaaagca cattgaagtt caattattag ctgacaacca cggtaacgtt     1380

gtccacttgt tcgaaagaga ctgttccgtc caaagacgtc accaaaaggt tgtcgaagtt     1440

gctccagcta agactttacc aagagaagtt agagatgcta tcttgaccga tgccgttaag     1500

ttggctaagg tttgtggtta cagaaacgct ggtactgctg aattcttggt tgacaaccaa     1560

aacagacatt acttcattga aatcaaccca agaattcaag tcgaacacac catcactgaa     1620

gaaatcactg gtattgacat tgtctccgct caaatccaaa tcgccgctgg tgctactttg     1680

actcaattag gtctattaca agacaaaatc accaccagag gtttctctat ccaatgtcgt     1740

atcaccactg aagatccatc caagaacttc caaccagaca ctggtcgttt ggaagtctac     1800

agatccgctg gtggtaacgg tgtcagattg gacggtggta acgcctacgc tggtgctacc     1860

atctctccac actacgactc catgttggtt aagtgttcct gttctggttc tacctacgaa     1920

attgtcagaa gaaagatgat cagagctttg attgaattca gaatcagagg tgtcaagacc     1980

aacatcccat tcttgttgac tttgttgacc aacccagttt tcattgaagg tacctactgg     2040

accactttca tcgatgacac tccacaattg ttccaaatgg tttcctctca aaacagagct     2100

caaaaattgt tgcactactt ggctgacttg gccgtcaacg gttcctctat caagggtcaa     2160

atcggtttac caaagttgaa gtccaaccct tccgttccac atttgcacga tgctcaaggt     2220

aatgtcatca acgttaccaa atctgcccca ccatccggtt ggagacaagt cttgttggaa     2280

aagggtccat ccgaatttgc caagcaagtc agacaattca acggtacttt gttgatggac     2340

accacctgga gagatgctca ccaatctttg ctagctacca gagtcagaac tcacgatttg     2400

gccaccattg ctccaaccac tgctcacgct ttggctggtg cctttgcttt ggaatgttgg     2460

ggtggtgcta ctttcgatgt cgccatgaga ttcttgcatg aggacccatg ggaaagattg     2520

agaaaattga gatctttggt cccaaacatt ccattccaaa tgttgttgag aggtgctaac     2580

ggtgttgctt actcctcttt gccagacaac gccattgacc atttcgttaa gcaagccaag     2640

gacaatggtg ttgacatttt cagagtcttt gacgctttga acgacttgga acaattgaag     2700

gttggtgtta atgctgtcaa gaaggctggt ggtgttgtcg aagctaccgt ttgttactct     2760

ggtgacatgt tgcaaccagg taagaaatac aacttggact actacttaga agttgtcgaa     2820

aagatcgttc aaatgggtac tcacatcttg ggtatcaagg acatggctgg taccatgaag     2880

ccagctgctg ccaaattgtt gattggttct ttacgtacca gatacccaga cttgccaatc     2940

cacgttcact ctcatgactc cgctggtact gctgttgctt ccatgactgc ttgtgctttg     3000

gccggtgctg atgttgttga cgttgccatt aactccatgt ccggtttgac ctctcaacca     3060

tctattaacg ctttgttggc ctccttggaa ggtaacattg acactggtat caacgtcgaa     3120

cacgttagag aattggacgc ttactgggct gaaatgagat tattatactc ttgtttcgaa     3180

gctgacttga agggtccaga ccctgaagtt taccaacacg aaattccagg tggtcaattg     3240

accaacttgt tgttccaagc tcaacaatta ggtctaggtg aacaatgggc tgaaaccaag     3300

agagcttaca gagaagctaa ctacttgttg ggtgacattg ttaaggtcac cccaacttct     3360

aaggtcgttg gtgatttggc tcaattcatg gtttctaaca aattgacttc tgatgacatc     3420

agaagattag ctaactcttt ggacttccca gactccgtta tggacttctt cgaaggtttg     3480

atcggtcaac catacggtgg tttcccagaa ccattgagat ccgatgtttt gagaaacaag     3540

cgtcgtaaat tgacttgtag accaggttta gaattggaac cattcgattt ggaaaagatc     3600

agagaagatt tgcaaaacag attcggtgat atcgatgaat gtgatgttgc ctcctacaac     3660

atgtatcctc gtgtctacga agatttccaa aagattagag aaacttacgg tgacttgtct     3720

gtcttaccaa ccaagaactt cttggctcca gctgaaccag acgaagaaat cgaagtcacc     3780

attgaacaag gtaagacttt gattatcaaa ttacaagctg ttggtgattt gaacaagaaa     3840

accggtcaaa gagaagtcta cttcgaattg aacggtgaat tgagaaagat cagagttgct     3900

gacaaatctc aaaacattca atctgttgcc aagccaaagg ctgatgtcca cgacacccac     3960

caaatcggtg ctccaatggc tggtgtcatc attgaagtca aggttcacaa gggttctttg     4020

gtcaagaagg gtgaatctat cgccgttttg tctgctatga agatggaaat ggttgtttcc     4080

tctccagctg atggtcaagt caaagatgtc tttatccgtg acggtgaatc cgtcgatgct     4140

tctgacttgt tggttgtttt ggaagaagaa actctaccac cttctcaaaa gaaataaagc     4200

gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata     4260

caaattttaa agtgactctt aggttttaaa acgaaaattc ttattcttga gtaactcttt     4320

cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc gctcttattg accacacctc     4380

taccggcatg ccgagcaaat gcctgcaaat cgctccccat ttcacccaat tgtagatatg     4440

ctaactccag caatgagttg atgaatctcg gtgtgtattt tatgtcctca gaggacaacc     4500

tcacgctttc cggcatcttc cagaccacag tatatccatc cgcctcctgt tg             4552


<210>  3
<211>  1572
<212>  DNA
<213>  Artificial sequence

<220>
<223>  KanMX selection marker

<400>  3
tcgtacgctg caggtcgacg aattctaccg ttcgtataat gtatgctata cgaagttata       60

gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg gaggcccaga      120

ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt      180

acatttagcc catacatccc catgtataat catttgcatc catacatttt gatggccgca      240

cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga aacgctcccc      300

tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata aaaggttagg      360

atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta ggatacagtt      420

ctcacatcac atccgaacat aaacaaccat gggtaaggaa aagactcacg tttcgaggcc      480

gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt      540

cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt      600

tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa      660

ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga      720

tgcatggtta ctcaccactg cgatccccgg caaaacagca ttccaggtat tagaagaata      780

tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc      840

gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca      900

atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg      960

gcctgttgaa caagtctgga aagaaatgca taagcttttg ccattctcac cggattcagt     1020

cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg     1080

ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg     1140

gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat     1200

tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc     1260

agtactgaca ataaaaagat tcttgttttc aagaacttgt catttgtata gtttttttat     1320

attgtagttg ttctatttta atcaaatgtt agcgtgattt atattttttt tcgcctcgac     1380

atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa tatcatgcgt caatcgtatg     1440

tgaatgctgg tcgctatact gctgtcgatt cgatactaac gccgccatcc agtgtcgaaa     1500

acgagctcat aacttcgtat aatgtatgct atacgaacgg tagaattcga tatcagatcc     1560

actagtggcc ta                                                         1572


<210>  4
<211>  2260
<212>  DNA
<213>  Aspergillus niger

<400>  4
aacgttgtcc aggtttgtat ccacgtgtgt ccgttccgcc aatattccgc gtgcccgcgg       60

aaccgccaga tattcattac ttgacgcaaa agcgtttgaa ataatgacga aaaagaagga      120

agaaaaaaaa agaaaaatac cgcttctagg cgggttatct actgatccga gcttccacta      180

ggatagcacc caaacacctg catatttgga cgacctttac ttacaccacc aaaaaccact      240

ttcgcctctc ccgcccctga taacgtccac taattgagcg attacctgag cggtcctctt      300

ttgtttgcag catgagactt gcatactgca aatcgtaagt agcaacgtgt caaggtcaaa      360

actgtatgga aaccttgtca cctcacttaa ttctagctag cctaccctgc aagtcaagag      420

gtgtccgtga ttcctagcca cctcaaggta tgcctctccc cggaaactgt ggccttttct      480

ggcacacatg atctccacga tttcaacata taaatagctt ttgataatgg caatattaat      540

caaatttatt ttacttcttt cttgtaacat ctctcttgta atcccttatt ccttctagct      600

atttttcata aaaaaccaag caactgctta tcaacacaca aacactaaat caaaatgaac      660

gttgaaactt ctttgccagg ttcttctggt tctgacttgg aaactttcca ccacgaaacc      720

aagaagcatg ccaaccacga ctctggtatt tccgtcaacc atgaagctga aattggtgtt      780

aaccacactt tcgaaaagcc aggtccagtt ggtatcagag aaagattacg tcacttcacc      840

tgggcttggt acactttgac catgtcctgt ggtggtttgg ctttgttgat tgtcaaccaa      900

ccacacgact tcaagggttt gaaagatatt gccagagttg tctactgttt gaacttggct      960

ttctttgtta tcgttacctc tttgatggcc atcagattca tcttgcacaa gaacatgtgg     1020

gaatccttgg gtcacgacag agaaggtttg tttttcccaa ctttctggtt atccattgct     1080

accatgatca ctggtttgta caagtgtttc ggtgatgatg ctaacgaaaa gttcaccaag     1140

tgtttgcaag ttttgttctg gatctactgt ggttgtacca tgatcactgc tgtcggtcaa     1200

tactctttcg tctttgctac ccacaaatac gaattgcaca ccatgatgcc atcctggatc     1260

ttgccagctt tcccagttat gttgtctggt actatcgcct ccgtcatcgg ttctggtcaa     1320

ccagcttccg atggtattcc aattattatt gctggtatca ctttccaagg tttaggtttc     1380

tccatctcct tcatgatgta cgctcactac attggtagat tgatggaagt tggtttacca     1440

tctccagaac acagaccagg tatgttcatc tgtgttggtc ctccagcttt caccgctttg     1500

gctttggtcg gtatggccaa ggctttacca gacgacttcc aaattgtcgg tgaccctcac     1560

gctgtcattg acggtcgtgt tatgttgttc ttggctgtct ctgctgccat cttcttatgg     1620

gctttgtctt tctggttctt ctgtatcgct gttgttgctg ttgtcagatc tccaccaaag     1680

ggtttccatt tgaactggtt tgccatggtt ttcccaaaca ctggtttcac cttggctacc     1740

atcactttgg ctaacatgtt cgaatctcca ggtgtcaagg gtgttgccac tgctatgtcc     1800

ctatgtgtca tcatcatgtt tattttcgtc ttggtttctg ccatcagagc tgtcatcaga     1860

aaggacatca tgtggccagg tcaagatgaa gatgtttctg aataaagagt aataattatt     1920

gcttccatat aatattttta tatacctctt atttttatgt attagttaat taagtatttt     1980

tatctatctg cttatcattt tcttttcata tagggggggt tggtgttttc ttgcccatca     2040

gattgatgtc ctccaactcg gcactatttt acaaagggtt tttttgtaag agaaggagaa     2100

gacagatact aaaccatacg ttactcgaaa caaaaaaaaa aaaaatggaa aaagctgcta     2160

tcaacaaaag acggcctcat caaacctaaa gaaaccatgt cagcgtcctc aaataaccac     2220

aaacatcctt cccatatgct cggtcgtgct tgttgtacct                           2260


<210>  5
<211>  2032
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  5
aaataaccac aaacatcctt cccatatgct cggtcgtgct tgttgtacct gtgcctactt       60

ggcttcacat acgttgcata cgtcgatata gataataatg ataatgacag caggattatc      120

gtaatacgta atagttgaaa atctcaaaaa tgtgtgggtc attacgtaaa taatgatagg      180

aatgggattc ttctattttt cctttttcca ttctagcagc cgtcgggaaa acgtggcatc      240

ctctctttcg ggctcaattg gagtcacgct gccgtgagca tcctctcttt ccatatctaa      300

caactgagca cgtaaccaat ggaaaagcat gagcttagcg ttgctccaaa aaagtattgg      360

atggttaata ccatttgtct gttctcttct gactttgact cctcaaaaaa aaaaaatcta      420

caatcaacag atcgcttcaa ttacgccctc acaaaaactt ttttccttct tcttcgccca      480

cgttaaattt tatccctcat gttgtctaac ggatttctgc acttgattta ttataaaaag      540

acaaagacat aatacttctc tatcaatttc agttattgtt cttccttgcg ttattcttct      600

gttcttcttt ttcttttgtc atatataacc ataaccaagt aatacatatt caaaatggtt      660

aaggttgcca tcttaggtgc ttctggtggt gtcggtcaac cattatctct attattgaaa      720

ttgtctccat acgtttctga attggctttg tacgatatca gagctgctga aggtattggt      780

aaggatttgt cccacatcaa caccaactcc tcttgtgttg gttacgacaa ggattccatc      840

gaaaacactt tgtccaatgc tcaagttgtc ttgattccag ctggtgttcc aagaaagcca      900

ggtttgacca gagatgattt gttcaagatg aacgctggta tcgttaagtc tttggttact      960

gctgtcggta aatttgcccc aaacgctcgt atcttagtca tctccaaccc tgttaactct     1020

ttggttccaa ttgccgttga aactttgaag aagatgggta agttcaagcc aggtaacgtt     1080

atgggtgtca ccaacttgga tttggtcaga gctgaaactt tcttggttga ctacttgatg     1140

ttgaagaacc caaagatcgg tcaagaacaa gacaagacca ccatgcacag aaaggtcacc     1200

gtcatcggtg gtcactctgg tgaaaccatc attccaatca tcactgacaa atccttggtt     1260

ttccaattgg acaagcaata cgaacatttc atccacagag tccaattcgg tggtgacgaa     1320

attgtcaagg ccaagcaagg tgccggttct gctaccttgt ccatggcttt cgctggtgcc     1380

aaatttgctg aagaagtctt acgttctttc cacaacgaaa agccagaaac tgaatctttg     1440

tctgctttcg tctacttgcc aggtttgaag aacggtaaga aggctcaaca attagtcggt     1500

gacaactcca ttgaatactt ctctttgcca attgttttga gaaacggttc cgttgtttcc     1560

attgacactt ctgttttgga aaaattgtct ccaagagaag aacaattggt caacactgct     1620

gtcaaggaat tgagaaagaa cattgaaaag ggtaagtctt tcatcttgga cagttaaagt     1680

ctgaagaatg aatgatttga tgatttcttt ttccctccat ttttcttact gaatatatca     1740

atgatataga cttgtatagt ttattatttc aaattaagta gctatatata gtcaagataa     1800

cgtttgtttg acacgattac attattcgtc gacatctttt ttcagcctgt cgtggtagca     1860

atttgaggag tattattaat tgaataggtt cattttgcgc tcgcataaac agttttcgtc     1920

agggacagta tgttggaatg agtggtaatt aatggtgaca tgacatgtta tagcaatacc     1980

tcgaaacctt cgaatccagc cagcatgtcg acacccacaa gatgtagtgc ac             2032


<210>  6
<211>  3056
<212>  DNA
<213>  Escherichia coli

<400>  6
gaaaccttcg aatccagcca gcatgtcgac acccacaaga tgtagtgcac gtgccgtaaa       60

aactaaaacg agcccccacc aaagaacaaa aaagaaggtg ctgggccccc actttcttcc      120

cttgcacgtg ataggaagat ggctacagaa acaagaagat ggaaatcgaa ggaaagaggg      180

agactggaag ctgtaaaaac tgaaatgaaa aaaaaaaaaa aaaaaaaaaa caagaagctg      240

aaaatggaag actgaaattt gaaaaatggt aaaaaaaaaa aagaaacacg aagctaaaaa      300

cctggattcc attttgagaa gaagcaagaa aggtaagtat ggtaacgacc gtacaggcaa      360

gcgcgaaggc aaatggaaaa gctggagtcc ggaagataat catttcatct tcttttgtta      420

gaacagaaca gtggatgtcc ctcatctcgg taacgtattg tccatgccct agaactctct      480

gtccctaaaa agaggacaaa aacccaatgg tttccccagc ttccagtgga gccaccgatc      540

ccactggaaa ccactggaca ggaagagaaa atcacggact tcctctattg aaggataatt      600

caacactttc accagatccc aaatgtcccg cccctattcc cgtgttccat cacgtaccat      660

aacttaccat ttcatcacgt tctctatggc acactggtac tgcttcgact gctttgcttc      720

atcttctcta tgggccaatg agctaatgag cacaatgtgc tgcgaaataa agggatatct      780

aatttatatt attacattat aatatgtact agtgtggtta ttggtaattg tacttaattt      840

tgatatataa agggtggatc tttttcattt tgaatcagaa ttggaattgc aacttgtctc      900

ttgtcactat tacttaatag taattatatt tcttattaac ctttttttta agtcaaaaca      960

ccaaggacaa gaactactct tcaaaggtat ttcaagttat catacgtgtc acacacgctt     1020

cacagtttca agtaaaaaaa aagaatatta cacaatgtcc aacaagcctt tcatctacca     1080

agctccattc ccaatgggta aggacaacac tgaatactac ttgttgactt ctgactacgt     1140

ttccgttgct gatttcgatg gtgaaaccat cttgaaggtt gaaccagaag ccttgacttt     1200

gttggctcaa caagccttcc acgatgcttc tttcatgttg cgtccagctc accaaaagca     1260

agttgctgcc attttgcacg acccagaagc ctccgaaaac gacaaatacg ttgctttgca     1320

attcttgaga aactctgaaa ttgctgccaa gggtgtctta ccaacttgtc aagacactgg     1380

tactgccatc attgtcggta agaagggtca aagagtctgg accggtggtg gtgacgaaga     1440

aactctatcc aagggtgttt acaacactta cattgaagat aatttacgtt actctcaaaa     1500

tgctgctttg gacatgtaca aggaagtcaa cactggtact aacttgccag ctcaaatcga     1560

cttatacgct gttgacggtg acgaatacaa gttcttgtgt gttgccaagg gtggtggttc     1620

tgctaacaag acctacttgt accaagaaac caaggctttg ttgactccag gtaaattgaa     1680

gaacttcttg gtcgaaaaga tgagaacttt gggtactgct gcttgtccac cataccacat     1740

tgctttcgtt atcggtggta cttccgctga aaccaacttg aaaaccgtca aattggcttc     1800

cgctcactac tacgatgaat tgccaactga aggtaacgaa cacggtcaag ccttcagaga     1860

tgtccaattg gaacaagaat tgttggaaga agctcaaaaa ttaggtttgg gtgctcaatt     1920

tggtggtaaa tactttgctc acgatatcag agttatcaga ttaccaagac atggtgcttc     1980

ttgtccagtt ggtatgggtg tttcctgttc tgctgacaga aacatcaagg ccaagatcaa     2040

cagagaaggt atctggattg aaaaattgga acacaaccca ggtcaataca tcccacaaga     2100

attgagacaa gctggtgaag gtgaagctgt caaggttgac ttgaacagac caatgaagga     2160

aatcttggct caattatctc aatacccagt ttccaccaga ttatctttga ccggtactat     2220

cattgtcggt cgtgacattg ctcatgccaa gttgaaggaa ttgattgatg ctggtaagga     2280

attgcctcaa tacatcaagg accatccaat ctactacgct ggtccagcca agaccccagc     2340

tggttaccca tctggttctt tgggtccaac caccgctggt agaatggact cttacgttga     2400

cttgctacaa tctcacggtg gttccatgat catgttggct aagggtaaca gatctcaaca     2460

agtcaccgat gcttgtcaca agcacggtgg tttctatttg ggttccattg gtggtccagc     2520

tgctgtcttg gctcaacaat ctatcaagca cttggaatgt gttgcttacc cagaattggg     2580

tatggaagcc atctggaaga ttgaagtcga agatttccca gctttcatct tagtcgatga     2640

caagggtaac gacttcttcc aacaaattgt caacaagcaa tgtgccaact gtaccaagta     2700

aaataaagca atcttgatga ggataatgat ttttttttga atatacataa atactaccgt     2760

ttttctgcta gattttgtga agacgtaaat aagtacatat tactttttaa gccaagacaa     2820

gattaagcat taactttacc cttttctctt ctaagtttca atactagtta tcactgttta     2880

aaagttatgg cgagaacgtc ggcggttaaa atatattacc ctgaacgtgg tgaattgaag     2940

ttctaggatg gtttaaagat ttttcctttt tgggaaataa gtaaacaata tattgctgcc     3000

ttcctcaaag ccaaagttcg cgttccgacc ttgcctccca aatccgagtt gcgatt         3056


<210>  7
<211>  1335
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nourseothricin selection marker

<400>  7
tcgtacgctg caggtcgacg aattctaccg ttcgtataat gtatgctata cgaagttata       60

gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg gaggcccaga      120

ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt      180

acatttagcc catacatccc catgtataat catttgcatc catacatttt gatggccgca      240

cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga aacgctcccc      300

tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata aaaggttagg      360

atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta ggatacagtt      420

ctcacatcac atccgaacat aaacaaccat gggtaccact cttgacgaca cggcttaccg      480

gtaccgcacc agtgtcccgg gggacgccga ggccatcgag gcactggatg ggtccttcac      540

caccgacacc gtcttccgcg tcaccgccac cggggacggc ttcaccctgc gggaggtgcc      600

ggtggacccg cccctgacca aggtgttccc cgacgacgaa tcggacgacg aatcggacga      660

cggggaggac ggcgacccgg actcccggac gttcgtcgcg tacggggacg acggcgacct      720

ggcgggcttc gtggtcgtct cgtactccgg ctggaaccgc cggctgaccg tcgaggacat      780

cgaggtcgcc ccggagcacc gggggcacgg ggtcgggcgc gcgttgatgg ggctcgcgac      840

ggagttcgcc cgcgagcggg gcgccgggca cctctggctg gaggtcacca acgtcaacgc      900

accggcgatc cacgcgtacc ggcggatggg gttcaccctc tgcggcctgg acaccgccct      960

gtacgacggc accgcctcgg acggcgagca ggcgctctac atgagcatgc cctgccccta     1020

atcagtactg acaataaaaa gattcttgtt ttcaagaact tgtcatttgt atagtttttt     1080

tatattgtag ttgttctatt ttaatcaaat gttagcgtga tttatatttt ttttcgcctc     1140

gacatcatct gcccagatgc gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt     1200

atgtgaatgc tggtcgctat actgctgtcg attcgatact aacgccgcca tccagtgtcg     1260

aaaacgagct cataacttcg tataatgtat gctatacgaa cggtagaatt cgatatcaga     1320

tccactagtg gccta                                                      1335


<210>  8
<211>  4429
<212>  DNA
<213>  Trypanosoma brucei

<400>  8
aaagccaaag ttcgcgttcc gaccttgcct cccaaatccg agttgcgatt gtgcttagtc       60

aaaaaattag ccttttaatt ctgctgtaac ccgtacatgc ccaaaatagg gggcgggtta      120

cacagaatat ataacatcgt aggtgtctgg gtgaacagtt tattcctggc atccactaaa      180

tataatggag cccgcttttt aagctggcat ccagaaaaaa aaagaatccc agcaccaaaa      240

tattgttttc ttcaccaacc atcagttcat aggtccattc tcttagcgca actacagaga      300

acaggggcac aaacaggcaa aaaacgggca caacctcaat ggagtgatgc aacctgcctg      360

gagtaaatga tgacacaagg caattgaccc acgcatgtat ctatctcatt ttcttacacc      420

ttctattacc ttctgctctc tctgatttgg aaaaagctga aaaaaaaggt tgaaaccagt      480

tccctgaaat tattccccta cttgactaat aagtatataa agacggtagg tattgattgt      540

aattctgtaa atctatttct taaacttctt aaattctact tttatagtta gtcttttttt      600

tagttttaaa acaccaagaa cttagtttcg aataaacaca cataaacaaa caaaatggtt      660

gatggtagat cttctgcttc cattgttgcc gttgacccag aaagagctgc cagagaaaga      720

gatgctgctg ccagagcttt gttgcaagac tctccattgc acaccaccat gcaatacgct      780

acctctggtt tggaattgac tgttccatac gctttgaagg ttgttgcttc tgctgacact      840

ttcgacagag ccaaggaagt tgctgatgaa gtcttgagat gtgcctggca attggctgac      900

accgttttga actctttcaa cccaaactct gaagtctctt tagtcggtag attaccagtc      960

ggtcaaaagc atcaaatgtc tgctccattg aaacgtgtca tggcttgttg tcaaagagtc     1020

tacaactcct ctgctggttg tttcgaccca tccactgctc cagttgccaa ggctttgaga     1080

gaaattgctt tgggtaagga aagaaacaat gcttgtttgg aagctttgac tcaagcttgt     1140

accttgccaa actctttcgt cattgatttc gaagctggta ctatctccag aaagcacgaa     1200

cacgcttctt tggatttggg tggtgtttcc aagggttaca tcgtcgatta cgtcattgac     1260

aacatcaatg ctgctggttt ccaaaacgtt ttctttgact ggggtggtga ctgtcgtgcc     1320

tccggtatga acgccagaaa cactccatgg gttgtcggta tcactagacc tccttccttg     1380

gacatgttgc caaaccctcc aaaggaagct tcttacatct ccgtcatctc tttggacaat     1440

gaagctttgg ctacctctgg tgattacgaa aacttgatct acactgctga cgataaacca     1500

ttgacctgta cctacgattg gaaaggtaag gaattgatga agccatctca atccaatatc     1560

gctcaagttt ccgtcaagtg ttactctgcc atgtacgctg acgctttggc taccgcttgt     1620

ttcatcaagc gtgacccagc caaggtcaga caattgttgg atggttggag atacgttaga     1680

gacaccgtca gagattaccg tgtctacgtc agagaaaacg aaagagttgc caagatgttc     1740

gaaattgcca ctgaagatgc tgaaatgaga aagagaagaa tttccaacac tttaccagct     1800

cgtgtcattg ttgttggtgg tggtttggct ggtttgtccg ctgccattga agctgctggt     1860

tgtggtgctc aagttgtttt gatggaaaag gaagccaagt tgggtggtaa ctctgccaag     1920

gctacctctg gtatcaacgg ttggggtact agagcccaag ctaaggcttc cattgtcgat     1980

ggtggtaagt acttcgaaag agatacctac aagtctggta tcggtggtaa caccgatcca     2040

gctttggtta agactttgtc catgaaatct gctgacgcta tcggttggtt gacttctcta     2100

ggtgttccat tgactgtttt gtcccaatta ggtggtcact ccagaaagag aactcacaga     2160

gccccagaca agaaggatgg tactccattg ccaattggtt tcaccatcat gaaaacttta     2220

gaagatcatg ttagaggtaa cttgtccggt agaatcacca tcatggaaaa ctgttccgtt     2280

acctctttgt tgtctgaaac caaggaaaga ccagacggta ctaagcaaat cagagttacc     2340

ggtgtcgaat tcactcaagc tggttctggt aagaccacca ttttggctga tgctgttatc     2400

ttggccaccg gtggtttctc caacgacaag actgctgatt ctttgttgag agaacatgcc     2460

ccacacttgg ttaacttccc aaccaccaac ggtccatggg ctactggtga tggtgtcaag     2520

ttggctcaaa gattaggtgc tcaattggtc gatatggaca aggttcaatt gcacccaact     2580

ggtttgatca acccaaagga cccagccaac ccaaccaaat tcttgggtcc agaagctcta     2640

agaggttctg gtggtgtttt gttgaacaaa caaggtaaga gatttgtcaa cgaattggat     2700

ttgagatctg ttgtttccaa ggccatcatg gaacaaggtg ctgaataccc aggttctggt     2760

ggttccatgt ttgcttactg tgtcttgaac gctgctgctc aaaaattgtt tggtgtttcc     2820

tctcacgaat tctactggaa gaagatgggt ttgttcgtca aggctgacac catgagagac     2880

ttggctgctt tgattggttg tccagttgaa tccgttcaac aaactttaga agaatacgaa     2940

agattatcca tctctcaaag atcttgtcca attaccagaa aatctgttta cccatgtgtt     3000

ttgggtacta aaggtccata ctatgtcgcc tttgtcactc catctatcca ctacaccatg     3060

ggtggttgtt tgatttctcc atctgctgaa atccaaatga agaacacttc ttccagagcc     3120

ccattgtccc actccaaccc aatcttgggt ttattcggtg ctggtgaagt caccggtggt     3180

gtccacggtg gtaacagatt aggtggtaac tctttgttgg aatgtgttgt tttcggtaga     3240

attgccggtg acagagcttc taccattttg caaagaaagt cctctgcttt gtctttcaag     3300

gtctggacca ctgttgtttt gagagaagtc agagaaggtg gtgtctacgg tgctggttcc     3360

cgtgtcttga gattcaactt accaggtgct ctacaaagat ctggtctatc cttgggtcaa     3420

ttcattgcca tcagaggtga ctgggacggt caacaattga ttggttacta ctctccaatc     3480

actttgccag acgatttggg tatgattgac attttggcca gatctgacaa gggtacttta     3540

cgtgaatgga tctctgcttt ggaaccaggt gacgctgtcg aaatgaaggc ttgtggtggt     3600

ttggtcatcg aaagaagatt atctgacaag cacttcgttt tcatgggtca cattatcaac     3660

aagctatgtt tgattgctgg tggtactggt gttgctccaa tgttgcaaat catcaaggcc     3720

gctttcatga agccattcat cgacactttg gaatccgtcc acttgatcta cgctgctgaa     3780

gatgtcactg aattgactta cagagaagtt ttggaagaac gtcgtcgtga atccagaggt     3840

aaattcaaga aaactttcgt tttgaacaga cctcctccat tatggactga cggtgtcggt     3900

ttcatcgacc gtggtatctt gaccaaccac gttcaaccac catctgacaa cttattggtt     3960

gccatctgtg gtccaccagt tatgcaaaga attgtcaagg ccactttaaa gactttaggt     4020

tacaacatga acttggtcag aaccgttgac gaaactgaac catctggaag ttaaaggaag     4080

tatctcggaa atattaattt aggccatgtc cttatgcacg tttcttttga tacttacggg     4140

tacatgtaca caagtatatc tatatatata aattaatgaa aatcccctat ttatatatat     4200

gactttaacg agacagaaca gttttttatt ttttatccta tttgatgaat gatacagttt     4260

cttattcacg tgttataccc acaccaaatc caatagcaat accggccatc acaatcactg     4320

tttcggcagc ccctaagatc agacaaaaca tccggaacca ccttaaatca acgtccctca     4380

gaaagcctgt atgcgaagcc acaatccttt ccaacagacc atactaagt                 4429


<210>  9
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  9
cattatatcg aggaaagccc                                                   20


<210>  10
<211>  75
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  10
aaagcaaagg aaggagagaa cagaggagta cttgtacgtt cgatgggcaa agaaagagac       60

acaaaactac gtggg                                                        75


<210>  11
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  11
ttgcccatcg aacgtacaag                                                   20


<210>  12
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  12
tgctaagatt tgtgttcgtt tgg                                               23


<210>  13
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  13
cggatcgatg tacacaaccg                                                   20


<210>  14
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  14
caacaggagg cggatggata tac                                               23


<210>  15
<211>  79
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  15
acgctttccg gcatcttcca gaccacagta tatccatccg cctcctgttg tcgtacgctg       60

caggtcgacg aattctacc                                                    79


<210>  16
<211>  75
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  16
gcggaatatt ggcggaacgg acacacgtgg atacaaacct ggacaacgtt taggccacta       60

gtggatctga tatcg                                                        75


<210>  17
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer
<400>  17
aacgttgtcc aggtttgtat cc                                                22


<210>  18
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  18
aggtacaaca agcacgaccg                                                   20


<210>  19
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  19
aaataaccac aaacatcctt ccc                                               23


<210>  20
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  20
gtgcactaca tcttgtgggt gtc                                               23


<210>  21
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  21
gaaaccttcg aatccagcca gc                                                22


<210>  22
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  22
aatcgcaact cggatttggg                                                   20


<210>  23
<211>  73
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  23
aaagccaaag ttcgcgttcc gaccttgcct cccaaatccg agttgcgatt tcttggcggc       60

cgtacgcata tag                                                          73


<210>  24
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  24
cgcacaccac gaggtgaagg                                                   20


<210>  25
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  25
cggcattatt gtgtatggct caata                                             25


<210>  26
<211>  75
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  26
gaacttcgac ctgttgcaat acttcgggtt cggcacaaac gtgtacggat agggtttcaa       60

agatccatac ttctc                                                        75


<210>  27
<211>  79
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer 

<400>  27
atccgtacac gtttgtgccg aacccgaagt attgcaacag gtcgaagttc tcgtacgctg       60

caggtcgacg aattctacc                                                    79


<210>  28
<211>  74
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  28
aatcgcaact cggatttggg aggcaaggtc ggaacgcgaa ctttggcttt aggccactag       60

tggatctgat atcg                                                         74


<210>  29
<211>  90
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer 

<400>  29
agaaagcctg tatgcgaagc cacaatcctt tccaacagac catactaagt attttatttt       60

acttttttta gaatgacctg ttcccgacac                                        90


<210>  30
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  30
cacaagctta ttcttccaaa aatc                                              24


<210>  31
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  31
aaagccaaag ttcgcgttcc                                                   20


<210>  32
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  32
acttagtatg gtctgttgga aagg                                              24


<210>  33
<211>  1139
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FRDg protein sequence (minus C-terminal SKI)

<400>  33

Met Val Asp Gly Arg Ser Ser Ala Ser Ile Val Ala Val Asp Pro Glu 
1               5                   10                  15      


Arg Ala Ala Arg Glu Arg Asp Ala Ala Ala Arg Ala Leu Leu Gln Asp 
            20                  25                  30          


Ser Pro Leu His Thr Thr Met Gln Tyr Ala Thr Ser Gly Leu Glu Leu 
        35                  40                  45              


Thr Val Pro Tyr Ala Leu Lys Val Val Ala Ser Ala Asp Thr Phe Asp 
    50                  55                  60                  


Arg Ala Lys Glu Val Ala Asp Glu Val Leu Arg Cys Ala Trp Gln Leu 
65                  70                  75                  80  


Ala Asp Thr Val Leu Asn Ser Phe Asn Pro Asn Ser Glu Val Ser Leu 
                85                  90                  95      


Val Gly Arg Leu Pro Val Gly Gln Lys His Gln Met Ser Ala Pro Leu 
            100                 105                 110         


Lys Arg Val Met Ala Cys Cys Gln Arg Val Tyr Asn Ser Ser Ala Gly 
        115                 120                 125             


Cys Phe Asp Pro Ser Thr Ala Pro Val Ala Lys Ala Leu Arg Glu Ile 
    130                 135                 140                 


Ala Leu Gly Lys Glu Arg Asn Asn Ala Cys Leu Glu Ala Leu Thr Gln 
145                 150                 155                 160 


Ala Cys Thr Leu Pro Asn Ser Phe Val Ile Asp Phe Glu Ala Gly Thr 
                165                 170                 175     


Ile Ser Arg Lys His Glu His Ala Ser Leu Asp Leu Gly Gly Val Ser 
            180                 185                 190         


Lys Gly Tyr Ile Val Asp Tyr Val Ile Asp Asn Ile Asn Ala Ala Gly 
        195                 200                 205             


Phe Gln Asn Val Phe Phe Asp Trp Gly Gly Asp Cys Arg Ala Ser Gly 
    210                 215                 220                 


Met Asn Ala Arg Asn Thr Pro Trp Val Val Gly Ile Thr Arg Pro Pro 
225                 230                 235                 240 


Ser Leu Asp Met Leu Pro Asn Pro Pro Lys Glu Ala Ser Tyr Ile Ser 
                245                 250                 255     


Val Ile Ser Leu Asp Asn Glu Ala Leu Ala Thr Ser Gly Asp Tyr Glu 
            260                 265                 270         


Asn Leu Ile Tyr Thr Ala Asp Asp Lys Pro Leu Thr Cys Thr Tyr Asp 
        275                 280                 285             


Trp Lys Gly Lys Glu Leu Met Lys Pro Ser Gln Ser Asn Ile Ala Gln 
    290                 295                 300                 


Val Ser Val Lys Cys Tyr Ser Ala Met Tyr Ala Asp Ala Leu Ala Thr 
305                 310                 315                 320 


Ala Cys Phe Ile Lys Arg Asp Pro Ala Lys Val Arg Gln Leu Leu Asp 
                325                 330                 335     


Gly Trp Arg Tyr Val Arg Asp Thr Val Arg Asp Tyr Arg Val Tyr Val 
            340                 345                 350         


Arg Glu Asn Glu Arg Val Ala Lys Met Phe Glu Ile Ala Thr Glu Asp 
        355                 360                 365             


Ala Glu Met Arg Lys Arg Arg Ile Ser Asn Thr Leu Pro Ala Arg Val 
    370                 375                 380                 


Ile Val Val Gly Gly Gly Leu Ala Gly Leu Ser Ala Ala Ile Glu Ala 
385                 390                 395                 400 


Ala Gly Cys Gly Ala Gln Val Val Leu Met Glu Lys Glu Ala Lys Leu 
                405                 410                 415     


Gly Gly Asn Ser Ala Lys Ala Thr Ser Gly Ile Asn Gly Trp Gly Thr 
            420                 425                 430         


Arg Ala Gln Ala Lys Ala Ser Ile Val Asp Gly Gly Lys Tyr Phe Glu 
        435                 440                 445             


Arg Asp Thr Tyr Lys Ser Gly Ile Gly Gly Asn Thr Asp Pro Ala Leu 
    450                 455                 460                 


Val Lys Thr Leu Ser Met Lys Ser Ala Asp Ala Ile Gly Trp Leu Thr 
465                 470                 475                 480 


Ser Leu Gly Val Pro Leu Thr Val Leu Ser Gln Leu Gly Gly His Ser 
                485                 490                 495     


Arg Lys Arg Thr His Arg Ala Pro Asp Lys Lys Asp Gly Thr Pro Leu 
            500                 505                 510         


Pro Ile Gly Phe Thr Ile Met Lys Thr Leu Glu Asp His Val Arg Gly 
        515                 520                 525             


Asn Leu Ser Gly Arg Ile Thr Ile Met Glu Asn Cys Ser Val Thr Ser 
    530                 535                 540                 


Leu Leu Ser Glu Thr Lys Glu Arg Pro Asp Gly Thr Lys Gln Ile Arg 
545                 550                 555                 560 


Val Thr Gly Val Glu Phe Thr Gln Ala Gly Ser Gly Lys Thr Thr Ile 
                565                 570                 575     


Leu Ala Asp Ala Val Ile Leu Ala Thr Gly Gly Phe Ser Asn Asp Lys 
            580                 585                 590         


Thr Ala Asp Ser Leu Leu Arg Glu His Ala Pro His Leu Val Asn Phe 
        595                 600                 605             


Pro Thr Thr Asn Gly Pro Trp Ala Thr Gly Asp Gly Val Lys Leu Ala 
    610                 615                 620                 


Gln Arg Leu Gly Ala Gln Leu Val Asp Met Asp Lys Val Gln Leu His 
625                 630                 635                 640 


Pro Thr Gly Leu Ile Asn Pro Lys Asp Pro Ala Asn Pro Thr Lys Phe 
                645                 650                 655     


Leu Gly Pro Glu Ala Leu Arg Gly Ser Gly Gly Val Leu Leu Asn Lys 
            660                 665                 670         


Gln Gly Lys Arg Phe Val Asn Glu Leu Asp Leu Arg Ser Val Val Ser 
        675                 680                 685             


Lys Ala Ile Met Glu Gln Gly Ala Glu Tyr Pro Gly Ser Gly Gly Ser 
    690                 695                 700                 


Met Phe Ala Tyr Cys Val Leu Asn Ala Ala Ala Gln Lys Leu Phe Gly 
705                 710                 715                 720 


Val Ser Ser His Glu Phe Tyr Trp Lys Lys Met Gly Leu Phe Val Lys 
                725                 730                 735     


Ala Asp Thr Met Arg Asp Leu Ala Ala Leu Ile Gly Cys Pro Val Glu 
            740                 745                 750         


Ser Val Gln Gln Thr Leu Glu Glu Tyr Glu Arg Leu Ser Ile Ser Gln 
        755                 760                 765             


Arg Ser Cys Pro Ile Thr Arg Lys Ser Val Tyr Pro Cys Val Leu Gly 
    770                 775                 780                 


Thr Lys Gly Pro Tyr Tyr Val Ala Phe Val Thr Pro Ser Ile His Tyr 
785                 790                 795                 800 


Thr Met Gly Gly Cys Leu Ile Ser Pro Ser Ala Glu Ile Gln Met Lys 
                805                 810                 815     


Asn Thr Ser Ser Arg Ala Pro Leu Ser His Ser Asn Pro Ile Leu Gly 
            820                 825                 830         


Leu Phe Gly Ala Gly Glu Val Thr Gly Gly Val His Gly Gly Asn Arg 
        835                 840                 845             


Leu Gly Gly Asn Ser Leu Leu Glu Cys Val Val Phe Gly Arg Ile Ala 
    850                 855                 860                 


Gly Asp Arg Ala Ser Thr Ile Leu Gln Arg Lys Ser Ser Ala Leu Ser 
865                 870                 875                 880 


Phe Lys Val Trp Thr Thr Val Val Leu Arg Glu Val Arg Glu Gly Gly 
                885                 890                 895     


Val Tyr Gly Ala Gly Ser Arg Val Leu Arg Phe Asn Leu Pro Gly Ala 
            900                 905                 910         


Leu Gln Arg Ser Gly Leu Ser Leu Gly Gln Phe Ile Ala Ile Arg Gly 
        915                 920                 925             


Asp Trp Asp Gly Gln Gln Leu Ile Gly Tyr Tyr Ser Pro Ile Thr Leu 
    930                 935                 940                 


Pro Asp Asp Leu Gly Met Ile Asp Ile Leu Ala Arg Ser Asp Lys Gly 
945                 950                 955                 960 


Thr Leu Arg Glu Trp Ile Ser Ala Leu Glu Pro Gly Asp Ala Val Glu 
                965                 970                 975     


Met Lys Ala Cys Gly Gly Leu Val Ile Glu Arg Arg Leu Ser Asp Lys 
            980                 985                 990         


His Phe Val Phe Met Gly His Ile  Ile Asn Lys Leu Cys  Leu Ile Ala 
        995                 1000                 1005             


Gly Gly  Thr Gly Val Ala Pro  Met Leu Gln Ile Ile  Lys Ala Ala 
    1010                 1015                 1020             


Phe Met  Lys Pro Phe Ile Asp  Thr Leu Glu Ser Val  His Leu Ile 
    1025                 1030                 1035             


Tyr Ala  Ala Glu Asp Val Thr  Glu Leu Thr Tyr Arg  Glu Val Leu 
    1040                 1045                 1050             


Glu Glu  Arg Arg Arg Glu Ser  Arg Gly Lys Phe Lys  Lys Thr Phe 
    1055                 1060                 1065             


Val Leu  Asn Arg Pro Pro Pro  Leu Trp Thr Asp Gly  Val Gly Phe 
    1070                 1075                 1080             


Ile Asp  Arg Gly Ile Leu Thr  Asn His Val Gln Pro  Pro Ser Asp 
    1085                 1090                 1095             


Asn Leu  Leu Val Ala Ile Cys  Gly Pro Pro Val Met  Gln Arg Ile 
    1100                 1105                 1110             


Val Lys  Ala Thr Leu Lys Thr  Leu Gly Tyr Asn Met  Asn Leu Val 
    1115                 1120                 1125             


Arg Thr  Val Asp Glu Thr Glu  Pro Ser Gly Ser 
    1130                 1135                 


<210>  34
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  34
ataaaaattg catatatgtg ggtagaaacc                                        30


<210>  35
<211>  78
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  35
tatagcatac attatacgaa cggtagaatt cgtcgacctg cagcgtacga tcaaagcaga       60

aatctgatgg gttcgaac                                                     78


<210>  36
<211>  75
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  36
agaaagcctg tatgcgaagc cacaatcctt tccaacagac catactaagt acaggtgatt       60

gtatgtgggc ttatg                                                        75


<210>  37
<211>  29
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  37
acattattgt aaaaacggag tagaaaggg                                         29


<210>  38
<211>  595
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR fragment 25, consisting of the 5' integration flank for 
       targeting the INT09.01 locus of CEN.PK113-7D

<400>  38
gggaaggaac ataatccggt atgtgaaatc aattgaatct atcctctgtc tcggtataat       60

attatacaag ttaggagaat agttatatat aaatatatgt gaacttgtag aactgatggc      120

atttgatggc cgtcacatgg tacgccacta aaaccttgat taagtagatt agattagact      180

agcaagtgtt ggtaacgttt tgtaggaaag agtctcaaag tacccagcgc caacttttgc      240

taaagtcaat cataaaatat atgtttttaa aatatgtagg tatgccgtgc atttttattc      300

ttttttaatg tttagctttc aaaaaagcta aaataaacca taagcgttat tcttattata      360

ttgatagtag aagaggaaga gtggttatct ttactcaaat taacgattgc ttttatttct      420

tacattaagg tcgaaactat ttgctactat gaaagccaag cagaaacaaa caagacctta      480

ttaagattga tgtttcaagc tatgcattcc caattcagaa gaacaaggga agagcataag      540

acggctcctc ggatcggcca gttgggagca aggtggtact tctgtcgtcg tattt           595


<210>  39
<211>  1381
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR fragment 26, which includes a nourseothricin selection marker
       functional in Saccharomyces cerevisiae.

<400>  39
tcctcggatc ggccagttgg gagcaaggtg gtacttctgt cgtcgtattt gtgctaccgt       60

tcgtataatg tatgctatac gaagttatgt ccccgccggg tcacccggcc agcgacatgg      120

aggcccagaa taccctcctt gacagtcttg acgtgcgcag ctcaggggca tgatgtgact      180

gtcgcccgta catttagccc atacatcccc atgtataatc atttgcatcc atacattttg      240

atggccgcac ggcgcgaagc aaaaattacg gctcctcgct gcagacctgc gagcagggaa      300

acgctcccct cacagacgcg ttgaattgtc cccacgccgc gcccctgtag agaaatataa      360

aaggttagga tttgccactg aggttcttct ttcatatact tccttttaaa atcttgctag      420

gatacagttc tcacatcaca tccgaacata aacaacaatg aatgggtacc actcttgacg      480

acacggctta ccggtaccgc accagtgtcc cgggggacgc cgaggccatc gaggcactgg      540

atgggtcctt caccaccgac accgtcttcc gcgtcaccgc caccggggac ggcttcaccc      600

tgcgggaggt gccggtggac ccgcccctga ccaaggtgtt ccccgacgac gaatcggacg      660

acgaatcgga cgacggggag gacggcgacc cggactcccg gacgttcgtc gcgtacgggg      720

acgacggcga cctggcgggc ttcgtggtcg tctcgtactc cggctggaac cgccggctga      780

ccgtcgagga catcgaggtc gccccggagc accgggggca cggggtcggg cgcgcgttga      840

tggggctcgc gacggagttc gcccgcgagc ggggcgccgg gcacctctgg ctggaggtca      900

ccaacgtcaa cgcaccggcg atccacgcgt accggcggat ggggttcacc ctctgcggcc      960

tggacaccgc cctgtacgac ggcaccgcct cggacggcga gcaggcgctc tacatgagca     1020

tgccctgccc ctaaataaat cagtactgac aataaaaaga ttcttgtttt caagaacttg     1080

tcatttgtat agttttttta tattgtagtt gttctatttt aatcaaatgt tagcgtgatt     1140

tatatttttt ttcgcctcga catcatctgc ccagatgcga agttaagtgc gcagaaagta     1200

atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat tcgatactaa     1260

cgccgccatc cagtgtcgaa aacgagctca taacttcgta taatgtatgc tatacgaacg     1320

gtacctccct catttggaac agcaggcgag gtgtattggt gcgagaagag gtgtttccga     1380

g                                                                     1381


<210>  40
<211>  4838
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR fragment 27 including coding sequence for fumarate reductase 
       from Trypanosoma brucei (FRDg) codon pair optimized for 
       expression in S. cerevisiae.

<400>  40
atttggaaca gcaggcgagg tgtattggtg cgagaagagg tgtttccgag gtgcgttcct       60

catcactaga agccgaactg ttgtcttcag tggggattgg ttcgacattt tgccaattgc      120

tgtcgatgta ccctttcaaa gccatgtacc ttaaatcttc atccttggca agtagattca      180

tcgggtgtgt ttgaagtaag aatatttgct tgtttttatg gtatcaaagg tatatgttgt      240

agaagacaat ttccggtaat ccaattgtct gtctgctcag tttagcacat gtatagtacg      300

ttgcacatag tctacaatat tcagcattca gcattcagta tacagcatat ggctaaatga      360

tcacaaatgt gattgatgat ttgacacgac tagaaaagag aacgaaaaag ggaaattcca      420

tgtcacgtgc gttggcacgt gacatggaat atcgaagaaa gaaaaaaaaa acgatctcgt      480

cctagtggaa gcccagagtc tggtcccccc ggagtcttcc caaaacaaga agctgacaca      540

tgttgacaca gaacacccca cagcaaatgc accacgctac gtagatcagg aagcttaact      600

ctagcgacct gtcgctcgcc ccacagaacc tcacccgaga accacacatt acacgccgcc      660

agctcccact atactcatct tgcttccctt aagcgttctc acgattcgtt cgctgccctt      720

cttcaagagt cttctgattc taattctcat tcgaaatcct ctacagttaa tgaattgctt      780

gacatgacat tcattgtctc atggttttgg ctttttggct tttgtctttt aaagctatat      840

caactttaca tataaatata cgtcaaaagg ggattcatta attagaaaat tctctttttc      900

aatagttgct attcattatc aatctattca actcaattgg ttattatttt catctttttg      960

tcatcctaaa ccatcaacaa tatttaaata tatctgttgc tacattaaga gttacttcag     1020

aaataacaaa aaaatcgatc aagaattaat aaaaatgaat ggtcgacggt agatcctctg     1080

cttctatcgt cgctgtcgac ccagaaagag ccgctcgtga acgtgacgct gctgctcgtg     1140

ctttgctaca agattctcca ttgcacacta ccatgcaata tgctacttcc ggtttggaat     1200

taaccgtccc atacgccttg aaggttgtcg cttctgctga caccttcgat cgtgctaagg     1260

aagttgctga cgaagtttta agatgtgctt ggcaattagc cgacactgtt ttaaactctt     1320

ttaacccaaa ctccgaagtc tctttggttg gtagattgcc agttggtcaa aaacaccaaa     1380

tgtccgctcc attgaagaga gtcatggcct gttgtcaacg tgtttacaac tcttctgctg     1440

gttgcttcga tccatctact gctccagtcg ccaaagcttt gagagaaatc gctttaggta     1500

aggaacgtaa caacgcttgc ttggaagctt tgacccaagc ttgtactcta ccaaactctt     1560

tcgttatcga cttcgaagct ggcaccattt ctagaaagca cgaacatgct tctttggact     1620

tgggtggtgt ctctaagggt tacattgttg actacgttat cgacaacatc aacgctgccg     1680

gtttccaaaa cgtcttcttc gactggggtg gcgactgtag agcttctggt atgaacgcta     1740

gaaacacccc atgggttgtt ggtattacca gaccaccatc tttggacatg ctaccaaacc     1800

caccaaagga agcttcctac atctctgtta tttctttgga caacgaagct ttggccactt     1860

ccggtgacta cgaaaacttg atttacaccg ctgatgacaa gccattgact tgtacttacg     1920

actggaaggg taaggaatta atgaagccat ctcagtccaa cattgctcaa gtctctgtta     1980

agtgttactc cgctatgtac gctgatgctt tagccactgc ttgtttcatt aagagagatc     2040

cagctaaggt tcgtcaattg ttggacggtt ggagatacgt ccgtgatact gttcgtgact     2100

acagagttta cgttcgtgaa aacgaaagag tcgctaagat gtttgaaatc gctaccgaag     2160

acgctgaaat gagaaagcgt agaatctcta acactttgcc agctagagtt attgtcgtcg     2220

gtggtggttt ggccggtttg tccgccgcta tcgaagctgc cggttgtggt gctcaggtcg     2280

tcttgatgga aaaagaagct aagttgggtg gtaacagcgc taaggctact tctggtatta     2340

acggttgggg caccagagcc caagccaaag cctctatcgt cgacggtggt aaatacttcg     2400

aacgtgacac ttacaagtct ggtattggtg gtaacactga cccagctttg gtcaagacct     2460

tgtccatgaa gtctgctgat gccatcggtt ggttaacctc cttgggtgtc ccattaaccg     2520

ttttgtctca attgggtggt cactctagaa agagaaccca ccgtgctcca gacaagaaag     2580

atggtactcc attaccaatc ggtttcacta tcatgaagac tttggaagac cacgttcgtg     2640

gtaacttgtc tggtcgtatc actatcatgg aaaactgtag cgtcacctct ctattatctg     2700

aaaccaagga acgtccagat ggtactaaac aaatccgtgt cactggtgtt gaatttaccc     2760

aagctggttc cggtaagact actatcttgg ctgatgctgt cattttggct actggtggtt     2820

tcagtaatga caagaccgct gactccttgt tgagagaaca cgctccacac ttggtcaact     2880

tcccaactac caacggccca tgggctaccg gtgacggtgt taagttggcc caaagattgg     2940

gtgctcaatt ggttgacatg gataaggttc aattgcatcc aactggtttg attaacccaa     3000

aggatccagc taacccaacc aagttcttgg gtccagaggc cttgagaggt tccggtggtg     3060

tcttgttgaa caagcaaggt aagagattcg tcaacgaatt agatctaaga tccgttgttt     3120

ccaaggctat catggagcaa ggtgccgaat acccaggttc cggtggttcc atgttcgctt     3180

actgtgtttt gaacgctgcc gctcaaaagt tattcggtgt ctcttctcat gaattctatt     3240

ggaagaagat gggtctattt gttaaggctg ataccatgag agatctagct gctttgatcg     3300

gctgtccagt tgaatctgtc caacaaacct tggaagaata cgaacgtttg tctatttctc     3360

aacgttcttg tccaatcacc cgtaagtctg tctacccatg tgtcttaggg accaagggtc     3420

catactacgt tgctttcgtc accccatcta ttcactacac tatgggcggt tgtttgatct     3480

ccccatccgc cgaaattcaa atgaaaaaca cttcctctcg tgctccattg tctcactcta     3540

atccaatctt gggtttgttc ggtgctggcg aagttaccgg tggtgttcat ggtggtaacc     3600

gtttgggtgg taactccttg ttggagtgtg ttgtcttcgg tagaatcgct ggtgacagag     3660

cctccaccat cttgcaacgt aagtcttctg ccttgtcctt caaggtttgg actaccgttg     3720

tcttgagaga agttagagaa ggtggcgtct acggtgccgg ttctagagtt ttgagattca     3780

acttgcctgg tgctttgcaa cgttccggtt tgtctttagg tcaattcatc gctatcagag     3840

gtgattggga cggtcaacaa ctaattggtt actactcccc aattactttg ccagatgact     3900

tgggtatgat cgatatctta gctcgttctg acaaaggtac tttgagagaa tggatttctg     3960

ctttggaacc tggtgatgct gttgaaatga aggcctgtgg tggtttggtt attgaaagaa     4020

gattgtccga caagcacttc gtcttcatgg gtcacatcat caacaaattg tgtttgattg     4080

ccggtggtac tggtgtcgcc ccaatgttgc aaattatcaa ggctgctttc atgaagcctt     4140

tcatcgatac cttggaatct gttcacttga tttacgctgc tgaggatgtt accgaattga     4200

cctacagaga agtcttggaa gaaagaagaa gagaatccag aggtaagttc aagaagactt     4260

ttgtcttaaa cagaccacca ccattgtgga ccgacggtgt tggtttcatc gatagaggta     4320

tcttgactaa ccacgttcaa cctccatccg acaacttgtt ggtcgctatc tgtggtcctc     4380

cagtcatgca aagaattgtt aaggctactt tgaaaacctt gggttacaat atgaacttgg     4440

ttagaactgt tgatgaaacc gaaccttccg gttaaataaa gcgaatttct tatgatttat     4500

gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc     4560

ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct     4620

ttctcaggta tagcatgagg tcgctcttat tgaccacacc tctaccggca tgccgagcaa     4680

atgcctgcaa atcgctcccc atttcaccca attgtagata tgctaactcc agcaatgagt     4740

tgatgaatct cggtgtgtat tttatgtcct cagaggacaa cctccctctt tccttgtacc     4800

aaagaacgag tatataccca ggtatccaga gcgcttgt                             4838


<210>  41
<211>  586
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR fragment 28 consisting of the 3' integration flank for 
       targeting the INT09.01 locus of CEN.PK113-7D

<400>  41
tttccttgta ccaaagaacg agtatatacc caggtatcca gagcgcttgt atctgcattt       60

aacagtagtg gtcactccta cagtagtcat acttctcaag cctactttcg gtatggcttt      120

taaagtcata aaataacgaa tagttcgtat accgctcatg tggcaaactc aatagcaaaa      180

tgtgatttca tatcacgaag ttttttatat tttttaatta tacaattgta atttgtatta      240

cggaaatgaa acatgtttgt atgttatgta gtatatcaat aattgattta ccgcatctgt      300

aaaagagaac catttatcac gcacatagtt ttagtattca ctaatcggtg ctctatggaa      360

gtacgagaca ccgtgcatag atggaagggg aaggtaataa gcttctctct aagaattttc      420

tttgtaaacc ctttcgactt ctgatttaac agcggctaag atttctcttc tctttaattt      480

ttgagcagat gtgacaaagc cattttcagg tgtccattct tcatcaaaga aaacaatacc      540

acataatagt tcaataccaa ccaaaccttg agatttggct gttgcg                     586



