                         SEQUENCE LISTING

<110>  New England Biolabs, Inc.
 
<120>  COMPOSITIONS AND METHODS FOR CAPPING RNA

<130>  NEB-374-PCT

<150>  61/912,367
<151>  2013-12-05

<150>  61/920,380
<151>  2013-12-23

<150>  62/002,564
<151>  2014-05-23

<150>  62/001,918
<151>  2014-06-13

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  844
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  1

Met Asp Ala Asn Val Val Ser Ser Ser Thr Ile Ala Thr Tyr Ile Asp 
1               5                   10                  15      


Ala Leu Ala Lys Asn Ala Ser Glu Leu Glu Gln Arg Ser Thr Ala Tyr 
            20                  25                  30          


Glu Ile Asn Asn Glu Leu Glu Leu Val Phe Ile Lys Pro Pro Leu Ile 
        35                  40                  45              


Thr Leu Thr Asn Val Val Asn Ile Ser Thr Ile Gln Glu Ser Phe Ile 
    50                  55                  60                  


Arg Phe Thr Val Thr Asn Lys Glu Gly Val Lys Ile Arg Thr Lys Ile 
65                  70                  75                  80  


Pro Leu Ser Lys Val His Gly Leu Asp Val Lys Asn Val Gln Leu Val 
                85                  90                  95      


Asp Ala Ile Asp Asn Ile Val Trp Glu Lys Lys Ser Leu Val Thr Glu 
            100                 105                 110         


Asn Arg Leu His Lys Glu Cys Leu Leu Arg Leu Ser Thr Glu Glu Arg 
        115                 120                 125             


His Ile Phe Leu Asp Tyr Lys Lys Tyr Gly Ser Ser Ile Arg Leu Glu 
    130                 135                 140                 


Leu Val Asn Leu Ile Gln Ala Lys Thr Lys Asn Phe Thr Ile Asp Phe 
145                 150                 155                 160 


Lys Leu Lys Tyr Phe Leu Gly Ser Gly Ala Gln Ser Lys Ser Ser Leu 
                165                 170                 175     


Leu His Ala Ile Asn His Pro Lys Ser Arg Pro Asn Thr Ser Leu Glu 
            180                 185                 190         


Ile Glu Phe Thr Pro Arg Asp Asn Glu Thr Val Pro Tyr Asp Glu Leu 
        195                 200                 205             


Ile Lys Glu Leu Thr Thr Leu Ser Arg His Ile Phe Met Ala Ser Pro 
    210                 215                 220                 


Glu Asn Val Ile Leu Ser Pro Pro Ile Asn Ala Pro Ile Lys Thr Phe 
225                 230                 235                 240 


Met Leu Pro Lys Gln Asp Ile Val Gly Leu Asp Leu Glu Asn Leu Tyr 
                245                 250                 255     


Ala Val Thr Lys Thr Asp Gly Ile Pro Ile Thr Ile Arg Val Thr Ser 
            260                 265                 270         


Asn Gly Leu Tyr Cys Tyr Phe Thr His Leu Gly Tyr Ile Ile Arg Tyr 
        275                 280                 285             


Pro Val Lys Arg Ile Ile Asp Ser Glu Val Val Val Phe Gly Glu Ala 
    290                 295                 300                 


Val Lys Asp Lys Asn Trp Thr Val Tyr Leu Ile Lys Leu Ile Glu Pro 
305                 310                 315                 320 


Val Asn Ala Ile Asn Asp Arg Leu Glu Glu Ser Lys Tyr Val Glu Ser 
                325                 330                 335     


Lys Leu Val Asp Ile Cys Asp Arg Ile Val Phe Lys Ser Lys Lys Tyr 
            340                 345                 350         


Glu Gly Pro Phe Thr Thr Thr Ser Glu Val Val Asp Met Leu Ser Thr 
        355                 360                 365             


Tyr Leu Pro Lys Gln Pro Glu Gly Val Ile Leu Phe Tyr Ser Lys Gly 
    370                 375                 380                 


Pro Lys Ser Asn Ile Asp Phe Lys Ile Lys Lys Glu Asn Thr Ile Asp 
385                 390                 395                 400 


Gln Thr Ala Asn Val Val Phe Arg Tyr Met Ser Ser Glu Pro Ile Ile 
                405                 410                 415     


Phe Gly Glu Ser Ser Ile Phe Val Glu Tyr Lys Lys Phe Ser Asn Asp 
            420                 425                 430         


Lys Gly Phe Pro Lys Glu Tyr Gly Ser Gly Lys Ile Val Leu Tyr Asn 
        435                 440                 445             


Gly Val Asn Tyr Leu Asn Asn Ile Tyr Cys Leu Glu Tyr Ile Asn Thr 
    450                 455                 460                 


His Asn Glu Val Gly Ile Lys Ser Val Val Val Pro Ile Lys Phe Ile 
465                 470                 475                 480 


Ala Glu Phe Leu Val Asn Gly Glu Ile Leu Lys Pro Arg Ile Asp Lys 
                485                 490                 495     


Thr Met Lys Tyr Ile Asn Ser Glu Asp Tyr Tyr Gly Asn Gln His Asn 
            500                 505                 510         


Ile Ile Val Glu His Leu Arg Asp Gln Ser Ile Lys Ile Gly Asp Ile 
        515                 520                 525             


Phe Asn Glu Asp Lys Leu Ser Asp Val Gly His Gln Tyr Ala Asn Asn 
    530                 535                 540                 


Asp Lys Phe Arg Leu Asn Pro Glu Val Ser Tyr Phe Thr Asn Lys Arg 
545                 550                 555                 560 


Thr Arg Gly Pro Leu Gly Ile Leu Ser Asn Tyr Val Lys Thr Leu Leu 
                565                 570                 575     


Ile Ser Met Tyr Cys Ser Lys Thr Phe Leu Asp Asp Ser Asn Lys Arg 
            580                 585                 590         


Lys Val Leu Ala Ile Asp Phe Gly Asn Gly Ala Asp Leu Glu Lys Tyr 
        595                 600                 605             


Phe Tyr Gly Glu Ile Ala Leu Leu Val Ala Thr Asp Pro Asp Ala Asp 
    610                 615                 620                 


Ala Ile Ala Arg Gly Asn Glu Arg Tyr Asn Lys Leu Asn Ser Gly Ile 
625                 630                 635                 640 


Lys Thr Lys Tyr Tyr Lys Phe Asp Tyr Ile Gln Glu Thr Ile Arg Ser 
                645                 650                 655     


Asp Thr Phe Val Ser Ser Val Arg Glu Val Phe Tyr Phe Gly Lys Phe 
            660                 665                 670         


Asn Ile Ile Asp Trp Gln Phe Ala Ile His Tyr Ser Phe His Pro Arg 
        675                 680                 685             


His Tyr Ala Thr Val Met Asn Asn Leu Ser Glu Leu Thr Ala Ser Gly 
    690                 695                 700                 


Gly Lys Val Leu Ile Thr Thr Met Asp Gly Asp Lys Leu Ser Lys Leu 
705                 710                 715                 720 


Thr Asp Lys Lys Thr Phe Ile Ile His Lys Asn Leu Pro Ser Ser Glu 
                725                 730                 735     


Asn Tyr Met Ser Val Glu Lys Ile Ala Asp Asp Arg Ile Val Val Tyr 
            740                 745                 750         


Asn Pro Ser Thr Met Ser Thr Pro Met Thr Glu Tyr Ile Ile Lys Lys 
        755                 760                 765             


Asn Asp Ile Val Arg Val Phe Asn Glu Tyr Gly Phe Val Leu Val Asp 
    770                 775                 780                 


Asn Val Asp Phe Ala Thr Ile Ile Glu Arg Ser Lys Lys Phe Ile Asn 
785                 790                 795                 800 


Gly Ala Ser Thr Met Glu Asp Arg Pro Ser Thr Arg Asn Phe Phe Glu 
                805                 810                 815     


Leu Asn Arg Gly Ala Ile Lys Cys Glu Gly Leu Asp Val Glu Asp Leu 
            820                 825                 830         


Leu Ser Tyr Tyr Val Val Tyr Val Phe Ser Lys Arg 
        835                 840                 


<210>  2
<211>  287
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  2

Met Asp Glu Ile Val Lys Asn Ile Arg Glu Gly Thr His Val Leu Leu 
1               5                   10                  15      


Pro Phe Tyr Glu Thr Leu Pro Glu Leu Asn Leu Ser Leu Gly Lys Ser 
            20                  25                  30          


Pro Leu Pro Ser Leu Glu Tyr Gly Ala Asn Tyr Phe Leu Gln Ile Ser 
        35                  40                  45              


Arg Val Asn Asp Leu Asn Arg Met Pro Thr Asp Met Leu Lys Leu Phe 
    50                  55                  60                  


Thr His Asp Ile Met Leu Pro Glu Ser Asp Leu Asp Lys Val Tyr Glu 
65                  70                  75                  80  


Ile Leu Lys Ile Asn Ser Val Lys Tyr Tyr Gly Arg Ser Thr Lys Ala 
                85                  90                  95      


Asp Ala Val Val Ala Asp Leu Ser Ala Arg Asn Lys Leu Phe Lys Arg 
            100                 105                 110         


Glu Arg Asp Ala Ile Lys Ser Asn Asn His Leu Thr Glu Asn Asn Leu 
        115                 120                 125             


Tyr Ile Ser Asp Tyr Lys Met Leu Thr Phe Asp Val Phe Arg Pro Leu 
    130                 135                 140                 


Phe Asp Phe Val Asn Glu Lys Tyr Cys Ile Ile Lys Leu Pro Thr Leu 
145                 150                 155                 160 


Phe Gly Arg Gly Val Ile Asp Thr Met Arg Ile Tyr Cys Ser Leu Phe 
                165                 170                 175     


Lys Asn Val Arg Leu Leu Lys Cys Val Ser Asp Ser Trp Leu Lys Asp 
            180                 185                 190         


Ser Ala Ile Met Val Ala Ser Asp Val Cys Lys Lys Asn Leu Asp Leu 
        195                 200                 205             


Phe Met Ser His Val Lys Ser Val Thr Lys Ser Ser Ser Trp Lys Asp 
    210                 215                 220                 


Val Asn Ser Val Gln Phe Ser Ile Leu Asn Asn Pro Val Asp Thr Glu 
225                 230                 235                 240 


Phe Ile Asn Lys Phe Leu Glu Phe Ser Asn Arg Val Tyr Glu Ala Leu 
                245                 250                 255     


Tyr Tyr Val His Ser Leu Leu Tyr Ser Ser Met Thr Ser Asp Ser Lys 
            260                 265                 270         


Ser Ile Glu Asn Lys His Gln Arg Arg Leu Val Lys Leu Leu Leu 
        275                 280                 285         


<210>  3
<211>  58
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  3

Val Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln 
            20                  25                  30          


Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys 
    50                  55              


<210>  4
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  4

Lys Arg Arg Trp Lys Lys Asn Phe Ile Ala Val Ser Ala Ala Asn Arg 
1               5                   10                  15      


Phe Lys Lys Ile Ser Ser Ser Gly Ala Leu 
            20                  25      


<210>  5
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  5

His His His His His His 
1               5       


<210>  6
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  6

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  7
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  7

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  8
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  8

Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  


<210>  9
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  9

Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr 
1               5                   10                  


<210>  10
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  10

Trp Ser His Pro Gln Phe Glu Lys 
1               5               


<210>  11
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  11

Met Asp Glu Lys Thr Thr Gly Trp Arg Gly Gly His Val Val Glu Gly 
1               5                   10                  15      


Leu Ala Gly Glu Leu Glu Gln Leu Arg Ala Arg Leu Glu His His Pro 
            20                  25                  30          


Gln Gly Gln Arg Glu Pro 
        35              


<210>  12
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  12

Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln His Met Asp Ser 
1               5                   10                  15  


<210>  13
<211>  51
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  13

Thr Asn Pro Gly Val Ser Ala Trp Gln Val Asn Thr Ala Tyr Thr Ala 
1               5                   10                  15      


Gly Gln Leu Val Thr Tyr Asn Gly Lys Thr Tyr Lys Cys Leu Gln Pro 
            20                  25                  30          


His Thr Ser Leu Ala Gly Trp Glu Pro Ser Asn Val Pro Ala Leu Trp 
        35                  40                  45              


Gln Leu Gln 
    50      


