                         SEQUENCE LISTING

<110>  LanzaTech New Zealand Limited
 
<120>  MICROORGANISMS AND METHODS FOR THE PRODUCTION OF KETONES

<130>  LT101PCT

<150>  US 61/911,449
<151>  2013-12-03

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  351
<212>  PRT
<213>  Clostridium autoethanogenum

<400>  1

Met Lys Gly Phe Ala Met Leu Gly Ile Asn Lys Leu Gly Trp Ile Glu 
1               5                   10                  15      


Lys Lys Asn Pro Val Pro Gly Pro Tyr Asp Ala Ile Val His Pro Leu 
            20                  25                  30          


Ala Val Ser Pro Cys Thr Ser Asp Ile His Thr Val Phe Glu Gly Ala 
        35                  40                  45              


Leu Gly Asn Arg Glu Asn Met Ile Leu Gly His Glu Ala Val Gly Glu 
    50                  55                  60                  


Ile Ala Glu Val Gly Ser Glu Val Lys Asp Phe Lys Val Gly Asp Arg 
65                  70                  75                  80  


Val Ile Val Pro Cys Thr Thr Pro Asp Trp Arg Ser Leu Glu Val Gln 
                85                  90                  95      


Ala Gly Phe Gln Gln His Ser Asn Gly Met Leu Ala Gly Trp Lys Phe 
            100                 105                 110         


Ser Asn Phe Lys Asp Gly Val Phe Ala Asp Tyr Phe His Val Asn Asp 
        115                 120                 125             


Ala Asp Met Asn Leu Ala Ile Leu Pro Asp Glu Ile Pro Leu Glu Ser 
    130                 135                 140                 


Ala Val Met Met Thr Asp Met Met Thr Thr Gly Phe His Gly Ala Glu 
145                 150                 155                 160 


Leu Ala Asp Ile Lys Met Gly Ser Ser Val Val Val Ile Gly Ile Gly 
                165                 170                 175     


Ala Val Gly Leu Met Gly Ile Ala Gly Ser Lys Leu Arg Gly Ala Gly 
            180                 185                 190         


Arg Ile Ile Gly Val Gly Ser Arg Pro Val Cys Val Glu Thr Ala Lys 
        195                 200                 205             


Phe Tyr Gly Ala Thr Asp Ile Val Asn Tyr Lys Asn Gly Asp Ile Val 
    210                 215                 220                 


Glu Gln Ile Met Asp Leu Thr His Gly Lys Gly Val Asp Arg Val Ile 
225                 230                 235                 240 


Met Ala Gly Gly Gly Ala Glu Thr Leu Ala Gln Ala Val Thr Met Val 
                245                 250                 255     


Lys Pro Gly Gly Val Ile Ser Asn Ile Asn Tyr His Gly Ser Gly Asp 
            260                 265                 270         


Thr Leu Pro Ile Pro Arg Val Gln Trp Gly Cys Gly Met Ala His Lys 
        275                 280                 285             


Thr Ile Arg Gly Gly Leu Cys Pro Gly Gly Arg Leu Arg Met Glu Met 
    290                 295                 300                 


Leu Arg Asp Leu Val Leu Tyr Lys Arg Val Asp Leu Ser Lys Leu Val 
305                 310                 315                 320 


Thr His Val Phe Asp Gly Ala Glu Asn Ile Glu Lys Ala Leu Leu Leu 
                325                 330                 335     


Met Lys Asn Lys Pro Lys Asp Leu Ile Lys Ser Val Val Thr Phe 
            340                 345                 350     


<210>  2
<211>  1056
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  2
atgaaaggtt ttgcaatgtt aggtattaac aaattaggat ggattgaaaa gaaaaaccca       60

gtgccaggtc cttatgatgc gattgtacat cctctagctg tatccccatg tacatcagat      120

atacatacgg tttttgaagg agcacttggt aatagggaaa atatgatttt aggccatgaa      180

gctgtaggtg aaatagccga agttggcagc gaagttaaag attttaaagt tggcgataga      240

gttatcgtac catgcacaac acctgactgg agatctttag aagtccaagc tggttttcag      300

cagcattcaa acggtatgct tgcaggatgg aagttttcca attttaaaga cggtgtattt      360

gcagattact ttcatgtaaa cgatgcagat atgaatcttg ccatactccc agatgaaata      420

cctttagaaa gtgcagttat gatgacagac atgatgacta ctggttttca tggagcagaa      480

cttgcagaca taaaaatggg ctccagcgtt gtagtaattg gtataggagc tgttggatta      540

atgggaatag ccggttccaa acttcgagga gcaggcagaa ttatcggtgt tggaagcaga      600

cctgtttgtg ttgaaacagc taaattttat ggagcaactg atattgtaaa ttataaaaat      660

ggtgatatag ttgaacaaat catggactta actcatggta aaggtgtaga ccgtgtaatc      720

atggcaggcg gtggtgctga aacactagca caagcagtaa ctatggttaa acctggcggc      780

gtaatttcta acatcaacta ccatggaagc ggtgatactt taccaatacc tcgtgttcaa      840

tggggctgcg gcatggctca caaaactata agaggaggat tatgccccgg cggacgtctt      900

agaatggaaa tgctaagaga tcttgttcta tataaacgtg ttgatttgag taaacttgtt      960

actcatgtat ttgatggtgc agaaaatatt gaaaaggccc ttttgcttat gaaaaataag     1020

ccaaaagatt taattaaatc agtagttaca ttctaa                               1056


<210>  3
<211>  1056
<212>  DNA
<213>  Clostridium ragsdalei

<400>  3
atgaaaggtt ttgcaatgtt aggtattaac aagttaggat ggattgaaaa gaaaaaccca       60

gtaccaggtc cttatgatgc gattgtacat cctctagctg tatccccatg tacatcagat      120

atacatacgg tttttgaagg agcacttggt aatagggaaa atatgatttt aggtcacgaa      180

gctgtaggtg aaatagctga agttggcagt gaagttaaag attttaaagt tggcgataga      240

gttatcgtac catgcacaac acctgactgg agatccttag aagtccaagc tggttttcaa      300

cagcattcaa acggtatgct tgcaggatgg aagttttcca attttaaaga cggtgtattt      360

gcagattact ttcatgtaaa cgatgcagat atgaatcttg caatacttcc agatgaaata      420

cctttagaaa gtgcagttat gatgacagac atgatgacta ctggttttca tggggcagaa      480

cttgctgaca taaaaatggg ttccagtgtt gtcgtaattg gtataggagc tgttggatta      540

atgggaatag ccggttccaa acttcgagga gcaggtagaa ttatcggtgt tggaagcaga      600

cccgtttgtg ttgaaacagc taaattttat ggagcaactg atattgtaaa ttataaaaat      660

ggtgatatag ttgaacaaat aatggactta actcatggta aaggtgtaga ccgtgtaatc      720

atggcaggcg gtggtgctga aacactagca caagcagtaa ctatggttaa acctggcggc      780

gtaatttcta acatcaacta ccatggaagc ggtgatactt tgccaatacc tcgtgttcaa      840

tggggctgcg gcatggctca caaaactata agaggagggt tatgtcccgg cggacgtctt      900

agaatggaaa tgctaagaga ccttgttcta tataaacgtg ttgatttgag caaacttgtt      960

actcatgtat ttgatggtgc agaaaatatt gaaaaggccc ttttgcttat gaaaaataag     1020

ccaaaagatt taattaaatc agtagttaca ttctaa                               1056


<210>  4
<211>  344
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Intron targeting region

<400>  4
aagcttataa ttatccttag atatcaatct tgtgcgccca gatagggtgt taagtcaagt       60

agtttaaggt actactctgt aagataacac agaaaacagc caacctaacc gaaaagcgaa      120

agctgatacg ggaacagagc acggttggaa agcgatgagt tacctaaaga caatcgggta      180

cgactgagtc gcaatgttaa tcagatataa ggtataagtt gtgtttactg aacgcaagtt      240

tctaatttcg attatatctc gatagaggaa agtgtctgaa acctctagta caaagaaagg      300

taagttagca agattgactt atctgttatc accacatttg taca                       344


<210>  5
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  156F primer

<400>  5
gccaggtcct tatgatgcga                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  939R primer

<400>  6
gccccattga acacgaggta                                                   20


<210>  7
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CatR primer

<400>  7
ttcgtttaca aaacggcaaa tgtga                                             25


<210>  8
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  RepHF primer

<400>  8
gccccattga acacgaggta                                                   20


