                         SEQUENCE LISTING

<110>  Lewis, Jared C.
<110>  Poor, Catherine
<110>  Andorfer, Mary
<110>  Payne, James
 
<120>  Directed Evolution of a Regioselective Halogenase for Increased 
       Thermostability

<130>  ARCD.P0569WO

<140>  unknown
<141>  2014-11-26

<150>  61/909,951
<151>  2013-11-27

<160>  2     

<170>  PatentIn version 3.5

<210>  1
<211>  530
<212>  PRT
<213>  Lechevalieria aerocolonigenes

<400>  1

Met Ser Gly Lys Ile Asp Lys Ile Leu Ile Val Gly Gly Gly Thr Ala 
1               5                   10                  15      


Gly Trp Met Ala Ala Ser Tyr Leu Gly Lys Ala Leu Gln Gly Thr Ala 
            20                  25                  30          


Asp Ile Thr Leu Leu Gln Ala Pro Asp Ile Pro Thr Leu Gly Val Gly 
        35                  40                  45              


Glu Ala Thr Ile Pro Asn Leu Gln Thr Ala Phe Phe Asp Phe Leu Gly 
    50                  55                  60                  


Ile Pro Glu Asp Glu Trp Met Arg Glu Cys Asn Ala Ser Tyr Lys Val 
65                  70                  75                  80  


Ala Ile Lys Phe Ile Asn Trp Arg Thr Ala Gly Glu Gly Thr Ser Glu 
                85                  90                  95      


Ala Arg Glu Leu Asp Gly Gly Pro Asp His Phe Tyr His Ser Phe Gly 
            100                 105                 110         


Leu Leu Lys Tyr His Glu Gln Ile Pro Leu Ser His Tyr Trp Phe Asp 
        115                 120                 125             


Arg Ser Tyr Arg Gly Lys Thr Val Glu Pro Phe Asp Tyr Ala Cys Tyr 
    130                 135                 140                 


Lys Glu Pro Val Ile Leu Asp Ala Asn Arg Ser Pro Arg Arg Leu Asp 
145                 150                 155                 160 


Gly Ser Lys Val Thr Asn Tyr Ala Trp His Phe Asp Ala His Leu Val 
                165                 170                 175     


Ala Asp Phe Leu Arg Arg Phe Ala Thr Glu Lys Leu Gly Val Arg His 
            180                 185                 190         


Val Glu Asp Arg Val Glu His Val Gln Arg Asp Ala Asn Gly Asn Ile 
        195                 200                 205             


Glu Ser Val Arg Thr Ala Thr Gly Arg Val Phe Asp Ala Asp Leu Phe 
    210                 215                 220                 


Val Asp Cys Ser Gly Phe Arg Gly Leu Leu Ile Asn Lys Ala Met Glu 
225                 230                 235                 240 


Glu Pro Phe Leu Asp Met Ser Asp His Leu Leu Asn Asp Ser Ala Val 
                245                 250                 255     


Ala Thr Gln Val Pro His Asp Asp Asp Ala Asn Gly Val Glu Pro Phe 
            260                 265                 270         


Thr Ser Ala Ile Ala Met Lys Ser Gly Trp Thr Trp Lys Ile Pro Met 
        275                 280                 285             


Leu Gly Arg Phe Gly Thr Gly Tyr Val Tyr Ser Ser Arg Phe Ala Thr 
    290                 295                 300                 


Glu Asp Glu Ala Val Arg Glu Phe Cys Glu Met Trp His Leu Asp Pro 
305                 310                 315                 320 


Glu Thr Gln Pro Leu Asn Arg Ile Arg Phe Arg Val Gly Arg Asn Arg 
                325                 330                 335     


Arg Ala Trp Val Gly Asn Cys Val Ser Ile Gly Thr Ser Ser Cys Phe 
            340                 345                 350         


Val Glu Pro Leu Glu Ser Thr Gly Ile Tyr Phe Val Tyr Ala Ala Leu 
        355                 360                 365             


Tyr Gln Leu Val Lys His Phe Pro Asp Lys Ser Leu Asn Pro Val Leu 
    370                 375                 380                 


Thr Ala Arg Phe Asn Arg Glu Ile Glu Thr Met Phe Asp Asp Thr Arg 
385                 390                 395                 400 


Asp Phe Ile Gln Ala His Phe Tyr Phe Ser Pro Arg Thr Asp Thr Pro 
                405                 410                 415     


Phe Trp Arg Ala Asn Lys Glu Leu Arg Leu Ala Asp Gly Met Gln Glu 
            420                 425                 430         


Lys Ile Asp Met Tyr Arg Ala Gly Met Ala Ile Asn Ala Pro Ala Ser 
        435                 440                 445             


Asp Asp Ala Gln Leu Tyr Tyr Gly Asn Phe Glu Glu Glu Phe Arg Asn 
    450                 455                 460                 


Phe Trp Asn Asn Ser Asn Tyr Tyr Cys Val Leu Ala Gly Leu Gly Leu 
465                 470                 475                 480 


Val Pro Asp Ala Pro Ser Pro Arg Leu Ala His Met Pro Gln Ala Thr 
                485                 490                 495     


Glu Ser Val Asp Glu Val Phe Gly Ala Val Lys Asp Arg Gln Arg Asn 
            500                 505                 510         


Leu Leu Glu Thr Leu Pro Ser Leu His Glu Phe Leu Arg Gln Gln His 
        515                 520                 525             


Gly Arg 
    530 


<210>  2
<211>  1593
<212>  DNA
<213>  Lechevalieria aerocolonigenes

<400>  2
atgtccggca agattgacaa gatcctcatc gtcggcggcg gcaccgccgg atggatggcc       60

gcgtcctatc tcggcaaggc cctgcagggc accgcggaca tcacactgct gcaggcaccc      120

gacatcccga cgctcggggt cggcgaggcc acgatcccca atctgcagac ggcgttcttc      180

gacttcctcg gaatccccga ggacgagtgg atgcgggagt gcaacgcgag ctacaaggtc      240

gccatcaagt tcatcaactg gcgcaccgcg ggcgagggga cgtccgaggc ccgcgagctc      300

gacggagggc ccgaccactt ctaccactcc ttcggtctgc tcaagtacca cgagcagatt      360

ccgctgtcgc actactggtt cgaccgttcg taccggggga agaccgtcga gccgttcgac      420

tacgcctgct acaaggaacc cgtcatcctc gacgccaaca ggtcaccgcg caggctcgac      480

ggttccaagg tgacgaacta cgcgtggcac ttcgacgcgc acctcgtcgc cgacttcctg      540

cgccggttcg ccaccgagaa gctcggcgtg cgccacgtcg aggaccgcgt cgagcacgtc      600

cagcgcgacg ccaacggcaa catcgagtcg gttcgcacgg caacggggcg tgtcttcgat      660

gccgacctct tcgtcgactg ctcgggcttc cgcgggctgc tgatcaacaa ggcgatggag      720

gagcccttcc tcgacatgag cgatcacctg ctcaacgaca gcgccgtcgc cacccaggtg      780

ccgcacgacg acgacgcgaa cggtgtggaa ccgttcacct cggcgatcgc catgaagtcg      840

ggctggacgt ggaagatccc gatgctcggc aggttcggca ccgggtacgt ctactcgagc      900

cggttcgcca ccgaggacga ggcggtgcgc gagttctgcg agatgtggca cctcgacccg      960

gagacccagc ccctcaacag gatccggttc cgggtcggcc gcaaccggcg cgcgtgggtc     1020

ggcaactgcg tcagcatcgg cacgtcgtcg tgcttcgtgg aaccactgga gtcgacgggc     1080

atctacttcg tctacgccgc gctgtaccag ctggtgaagc acttccccga caagagcctc     1140

aaccccgtgc tgaccgccag gttcaaccgc gagatcgaga cgatgttcga cgacacgcgc     1200

gacttcatcc aggcgcactt ctacttctcg ccgcgcacgg acaccccgtt ctggagggcc     1260

aacaaggagc tgcgcctggc ggacggcatg caggagaaga tcgacatgta ccgcgcgggc     1320

atggcgatca acgcgcccgc gtccgacgac gcccagctct actacggcaa cttcgaggag     1380

gagttccgca acttctggaa caacagcaac tactactgcg tgctggccgg cctcggtctg     1440

gtgcccgacg caccctcacc acgcctggcg cacatgccac aggcgacgga gtcggtggac     1500

gaggtcttcg gcgccgtcaa ggaccggcag cggaacctgc tcgagaccct gccgagcctc     1560

cacgagttcc tgaggcaaca gcacggccgc tga                                  1593


