                         SEQUENCE LISTING

<110>  UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INCORPORATED
 
<120>  MODIFICATION OF THE XYLAN UTILIZATION SYSTEM FOR PRODUCTION OF 
       ACIDIC XYLOOLIGOSACCHARIDES FROM LIGNOCELLULOSICS

<130>  UF.1143XC1PCT

<150>  US 61/908,426
<151>  2013-11-25

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer used for the amplification of xynD-xynC-bglC' 
       genes from B. subtilis strain 168

<400>  1
gcatacctcg agcgtctggc aatggcggtg ta                                 32


<210>  2
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer used for the amplification of xynD-xynC-bglC' 
       genes from B. subtilis strain 168

<400>  2
agcagcagca atctacaacc t                                             21


<210>  3
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer used in the amplification of DNA containing xynA 
       genes from B. subtilis strain 168

<400>  3
ggagtgctcg agaggaggaa gtcatggtaa gc                                 32


<210>  4
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer used in the amplification of DNA containing xynA 
       genes from B. subtilis strain 168

<400>  4
gcgttgtcta gatcgtagag tccccattca taaat                              35


<210>  5
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer used for the amplification of DNA containing GH11 
       endoxylanase xynA gene from B. subtilis strain 168

<400>  5
atgtccctcg agagcacaga ctactggcaa aatt                               34


<210>  6
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer used for the amplification of DNA containing GH11 
       endoxylanase  xynA gene from B. subtilis strain 168

<400>  6
cgataaggat cccctacctc cagcaattcc aa                                 32


<210>  7
<211>  311
<212>  PRT
<213>  Bacillus subtilis

<400>  7

Met Gln Gln Asp Pro Ser Ile Ser Ser Lys Gln Lys Ser Thr Ala Val 
1               5                   10                  15      


Ile Lys Met Val Ile Ser Met Val Ile Phe Gly Ser Ile Gly Phe Phe 
            20                  25                  30          


Ser Glu His Thr Asn Leu Pro Ser Phe Glu Leu Val Phe Val Arg Cys 
        35                  40                  45              


Leu Cys Ala Thr Leu Phe Leu Gly Phe Cys Trp Leu Ala Ser Gly Gln 
    50                  55                  60                  


Tyr Lys Thr Glu Lys Trp Ser Lys Arg Asp Val Leu Gln Thr Leu Ala 
65                  70                  75                  80  


Cys Gly Phe Phe Leu Val Phe Asn Trp Val Phe Leu Phe Lys Ser Phe 
                85                  90                  95      


Glu Glu Thr Ser Val Thr Ile Ala Ile Ser Val Tyr His Leu Ala Pro 
            100                 105                 110         


Val Leu Val Leu Leu Leu Gly Ser Phe Phe Tyr Arg Glu Lys Leu Asn 
        115                 120                 125             


Val Ile Ser Val Ser Ser Ile Ile Ile Cys Phe Leu Gly Thr Ala Leu 
    130                 135                 140                 


Ile Ser Gly Ile Asn Gly Ser Thr Ser Leu Thr Gln Leu Met Gly Ser 
145                 150                 155                 160 


Gly Ile Ile Trp Ala Val Leu Ala Ala Leu Phe Tyr Ala Phe Thr Thr 
                165                 170                 175     


Leu Leu Gly Lys Gly Ile His Asn Leu Ser Pro Tyr Thr Thr Thr Phe 
            180                 185                 190         


Leu Gln Thr Gly Leu Gly Val Ile Ile Leu Ile Pro Phe Ile His Phe 
        195                 200                 205             


Gly Ala Phe Ala Asp Leu Ser Gln Gly Asn Trp Ile Met Val Val Ser 
    210                 215                 220                 


Thr Gly Ile Ile His Thr Gly Ile Val Tyr Leu Leu Phe Phe Asp Ser 
225                 230                 235                 240 


Leu Arg Phe Leu Ser Thr Lys Phe Ile Ser Ile Ile Val Phe Leu Asp 
                245                 250                 255     


Pro Ala Val Ala Ile Val Leu Asp Thr Val Phe Thr Gly Phe Arg Pro 
            260                 265                 270         


Asp Leu Tyr Gln Thr Leu Gly Ile Val Met Ile Phe Ala Gly Met Ala 
        275                 280                 285             


Leu Thr Leu Val Arg Arg Gln Gly Lys Ala Asn Val Thr Ala Glu Gly 
    290                 295                 300                 


Thr Asp Ile Glu Gln Ile Gln 
305                 310     


<210>  8
<211>  936
<212>  DNA
<213>  Bacillus subtilis

<400>  8
atgcaacagg atccttccat atcgtcaaaa caaaaatcaa cagccgtaat caaaatggtc   60

atctctatgg tgattttcgg ctccatcggc tttttctcag agcataccaa tctgccgtca  120

tttgaattgg tctttgtccg ctgcctttgc gcgacgctgt ttttaggctt ttgctggctg  180

gcgtccggcc agtataaaac tgaaaaatgg agcaagagag acgtgctgca aacattggcg  240

tgcggtttct ttcttgtatt caactgggtg ttcttattta agtcttttga agaaacgtcc  300

gttacaattg ccatctctgt ttatcatcta gcgccggtgc ttgttcttct tctggggagc  360

ttcttctaca gagagaaatt aaatgtgatc tccgtcagct ccattatcat ctgctttctt  420

gggactgcgc tgatttccgg gattaacggc agcacatcat tgacacagct gatgggatca  480

gggatcatat gggcagttct cgccgcactg ttttatgcgt tcaccacttt attgggaaaa  540

ggcattcaca atctcagtcc ttacacgacc accttcttgc agacaggttt aggggttatc  600

atcctgattc cgtttattca ctttggcgct tttgcggacc tatctcaggg aaactggatt  660

atggtggtat ctaccggcat catccatacg ggtattgtat atttgctgtt ttttgacagt  720

ttacgatttt tatcaacgaa atttatttca atcattgtat ttctcgaccc cgctgtcgcg  780

atcgtgctcg ataccgtctt cacaggcttc cgccctgacc tctatcaaac gcttggcatc  840

gtaatgatct ttgcgggcat ggccttgacg cttgtcagga ggcaggggaa ggcgaatgtg  900

acagctgagg gtacggatat tgaacaaata caataa                            936


<210>  9
<211>  428
<212>  PRT
<213>  Bacillus subtilis

<400>  9

Met Arg Lys Tyr Gln Ala Arg Ile Ile Ser Ile Ile Leu Ala Met Ile 
1               5                   10                  15      


Phe Ile Met Phe Trp Asp Tyr Leu Phe Tyr Phe Ile Gly Lys Asn Pro 
            20                  25                  30          


Ile Asn Trp Pro Val Asp Ile Val Tyr Thr Ala Val Thr Leu Val Ser 
        35                  40                  45              


Val Trp Met Leu Ala Tyr Tyr Ile Asp Glu Lys Gln Gln Leu Val Lys 
    50                  55                  60                  


Lys Met Lys Asp Asn Glu Trp Lys Tyr Lys Gln Leu Ser Glu Glu Lys 
65                  70                  75                  80  


Asn Arg Ile Met Asp Asn Leu Gln Glu Ile Val Phe Gln Thr Asn Ala 
                85                  90                  95      


Lys Gly Glu Ile Thr Tyr Leu Asn Gln Ala Trp Ala Ser Ile Thr Gly 
            100                 105                 110         


Phe Ser Ile Ser Glu Cys Met Gly Thr Met Tyr Asn Asp Tyr Phe Ile 
        115                 120                 125             


Lys Glu Lys His Val Ala Asp His Ile Asn Thr Gln Ile Gln Asn Lys 
    130                 135                 140                 


Ala Ser Ser Gly Met Phe Thr Ala Lys Tyr Val Thr Lys Asn Gly Thr 
145                 150                 155                 160 


Ile Phe Trp Gly Glu Val His Tyr Lys Leu Tyr Tyr Asp Arg Asp Asp 
                165                 170                 175     


Gln Phe Thr Gly Ser Leu Gly Thr Met Ser Asp Ile Thr Glu Arg Lys 
            180                 185                 190         


Glu Ala Glu Asp Glu Leu Ile Glu Ile Asn Glu Arg Leu Ala Arg Glu 
        195                 200                 205             


Ser Gln Lys Leu Ser Ile Thr Ser Glu Leu Ala Ala Gly Ile Ala His 
    210                 215                 220                 


Glu Val Arg Asn Pro Leu Thr Ser Val Ser Gly Phe Leu Gln Ile Met 
225                 230                 235                 240 


Lys Thr Gln Tyr Pro Asp Arg Lys Asp Tyr Phe Asp Ile Ile Phe Ser 
                245                 250                 255     


Glu Ile Lys Arg Ile Asp Leu Val Leu Ser Glu Leu Leu Leu Leu Ala 
            260                 265                 270         


Lys Pro Gln Ala Ile Thr Phe Lys Thr His Gln Leu Asn Glu Ile Leu 
        275                 280                 285             


Lys Gln Val Thr Thr Leu Leu Asp Thr Asn Ala Ile Leu Ser Asn Ile 
    290                 295                 300                 


Val Ile Glu Lys Asn Phe Lys Glu Thr Asp Gly Cys Met Ile Asn Gly 
305                 310                 315                 320 


Asp Glu Asn Gln Leu Lys Gln Val Phe Ile Asn Ile Ile Lys Asn Gly 
                325                 330                 335     


Ile Glu Ala Met Pro Lys Gly Gly Val Val Thr Ile Ser Thr Ala Lys 
            340                 345                 350         


Thr Ala Ser His Ala Val Ile Ser Val Lys Asp Glu Gly Asn Gly Met 
        355                 360                 365             


Pro Gln Glu Lys Leu Lys Gln Ile Gly Lys Pro Phe Tyr Ser Thr Lys 
    370                 375                 380                 


Glu Lys Gly Thr Gly Leu Gly Leu Pro Ile Cys Leu Arg Ile Leu Lys 
385                 390                 395                 400 


Glu His Asp Gly Glu Leu Lys Ile Glu Ser Glu Ala Gly Lys Gly Ser 
                405                 410                 415     


Val Phe Gln Val Val Leu Pro Leu Lys Ser Asp Ser 
            420                 425             


<210>  10
<211>  1287
<212>  DNA
<213>  Bacillus subtilis

<400>  10
atgagaaaat atcaagctcg tatcatttcc atcattttgg caatgatttt tataatgttt   60

tgggattatt tattttattt tataggcaaa aacccgatta attggcctgt tgatattgtg  120

tatactgcag tcacgctggt aagtgtctgg atgttggctt attatattga tgagaaacag  180

cagctggtta agaaaatgaa ggataacgaa tggaagtata aacagctttc tgaagaaaaa  240

aaccgcatca tggataattt gcaggaaatc gtatttcaaa cgaatgcaaa aggtgaaatt  300

acatatttaa accaagcgtg ggcatctata accgggtttt caatcagtga atgtatggga  360

acaatgtata acgattactt cataaaagaa aagcacgtag ccgaccacat taacacccaa  420

atccaaaaca aagcgtcttc tggcatgttt acggcaaaat acgtgacaaa aaacggcacg  480

attttttggg gagaagttca ttataaactt tactatgacc gggatgacca atttacaggc  540

agcctgggta caatgtcaga tatcactgag cggaaagagg ctgaagatga gctcattgag  600

attaatgaac ggctggcgag ggaatcccag aaactatcaa tcacaagtga acttgccgca  660

ggtattgctc atgaggtcag aaacccttta acatctgtca gcggtttcct ccagattatg  720

aaaacacaat atccggacag aaaagactat tttgacatca tcttttcaga gattaaaaga  780

atcgatttag tgctcagcga gctgctgctg cttgcaaaac cgcaggcaat cacatttaaa  840

acacaccagc ttaatgagat cttgaaacaa gtcacgacat tgcttgatac caatgcaatt  900

ctgtccaata tcgtcataga gaaaaatttc aaagagacag atggctgtat gattaatgga  960

gacgaaaatc agctgaagca ggtctttatc aacatcatta aaaacggaat tgaggcaatg 1020

ccaaagggcg gtgtcgtaac catttcaact gctaaaaccg cctctcatgc agtgataagc 1080

gtaaaggatg aaggaaacgg catgccgcag gaaaagctga agcagattgg caaacctttt 1140

tattcaacaa aagaaaaggg cactggactg ggacttccca tttgtttgag aatcctgaag 1200

gaacatgacg gggaattgaa aatcgaaagt gaagctggaa aaggcagcgt ctttcaagtg 1260

gttttgcctt taaaatcaga cagctga                                     1287


