                         SEQUENCE LISTING

<110>  The President and Fellows of Harvard College
 
<120>  MUTANT CAS9 PROTEINS

<130>  010498_00553

<140>  PCT/US2014/066375
<141>  2014-11-19

<150>  61/906,374
<151>  2013-11-19

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  2679
<212>  DNA
<213>  Neisseria meningitidis

<400>  1
atggccgcct tcaagcccaa ccccatcaac tacatcctgg gcctggccat cggcatcgcc       60

agcgtgggct gggccatggt ggagatcgac gaggacgaga accccatctg cctgatcgac      120

ctgggtgtgc gcgtgttcga gcgcgctgag gtgcccaaga ctggtgacag tctggctatg      180

gctcgccggc ttgctcgctc tgttcggcgc cttactcgcc ggcgcgctca ccgccttctg      240

cgcgctcgcc gcctgctgaa gcgcgagggt gtgctgcagg ctgccgactt cgacgagaac      300

ggcctgatca agagcctgcc caacactcct tggcagctgc gcgctgccgc tctggaccgc      360

aagctgactc ctctggagtg gagcgccgtg ctgctgcacc tgatcaagca ccgcggctac      420

ctgagccagc gcaagaacga gggcgagacc gccgacaagg agctgggtgc tctgctgaag      480

ggcgtggccg acaacgccca cgccctgcag actggtgact tccgcactcc tgctgagctg      540

gccctgaaca agttcgagaa ggagagcggc cacatccgca accagcgcgg cgactacagc      600

cacaccttca gccgcaagga cctgcaggcc gagctgatcc tgctgttcga gaagcagaag      660

gagttcggca acccccacgt gagcggcggc ctgaaggagg gcatcgagac cctgctgatg      720

acccagcgcc ccgccctgag cggcgacgcc gtgcagaaga tgtccggcgg cggttcgggc      780

gaccactacg gcaagaagaa caccgaggag aagatctacc tgcctcctat ccccgccgac      840

gagatccgca accccgtggt gctgcgcgcc ctgagccagg cccgcaaggt gatcaacggc      900

gtggtgcgcc gctacggcag ccccgcccgc atccacatcg agaccgcccg cgaggtgggc      960

aagagcttca aggaccgcaa ggagatcgag aagcgccagg aggagaaccg caaggaccgc     1020

gagaaggccg ccgccaagtt ccgcgagtac ttccccaact tcgtgggcga gcccaagagc     1080

aaggacatcc tgaagctgcg cctgtacgag cagcagcacg gcaagtgcct gtacagcggc     1140

aaggagatca acctgggccg cctgaacgag aagggctacg tggagatcgc cgctgccctg     1200

cccttcagcc gcacctggga cgacagcttc aacaacaagg tgctggtgct gggcagcgag     1260

gctcagaaca agggcaacca gaccccctac gagtacttca acggcaagga caacagccgc     1320

gagtggcagg agttcaaggc ccgcgtggag accagccgct tcccccgcag caagaagcag     1380

cgcatcctgc tgcagaagtt cgacgaggac ggcttcaagg agcgcaacct gaacgacacc     1440

cgctacgtga accgcttcct gtgccagttc gtggccgacc gcatgcgcct gaccggcaag     1500

ggcaagaagc gcgtgttcgc cagcaacggc cagatcacca acctgctgcg cggcttctgg     1560

ggcctgcgca aggtgcgcgc cgagaacgac cgccaccacg ccctggacgc cgtggtggtg     1620

gcctgcagca ccgtggccat gcagcagaag atcacccgct tcgtgcgcta caaggagatg     1680

aacgccttcg acggtaaaac catcgacaag gagaccggcg aggtgctgca ccagaagacc     1740

cacttccccc agccctggga gttcttcgcc caggaggtga tgatccgcgt gttcggcaag     1800

cccgacggca agcccgagtt cgaggaggcc gacacccccg agaagctgcg caccctgctg     1860

gccgagaagc tgagcagccg ccctgaggcc gtgcacgagt acgtgactcc tctgttcgtg     1920

agccgcgccc ccaaccgcaa gatgagcggt cagggtcaca tggagaccgt gaagagcgcc     1980

aagcgcctgg acgagggcgt gagcgtgctg cgcgtgcccc tgacccagct gaagctgaag     2040

gacctggaga agatggtgaa ccgcgagcgc gagcccaagc tgtacgaggc cctgaaggcc     2100

cgcctggagg cccacaagga cgaccccgcc aaggccttcg ccgagccctt ctacaagtac     2160

gacaaggccg gcaaccgcac ccagcaggtg aaggccgtgc gcgtggagca ggtgcagaag     2220

accggcgtgt gggtgcgcaa ccacaacggc atcgccgaca acgccaccat ggtgcgcgtg     2280

gacgtgttcg agaagggcga caagtactac ctggtgccca tctacagctg gcaggtggcc     2340

aagggcatcc tgcccgaccg cgccgtggtg cagggcaagg acgaggagga ctggcagctg     2400

atcgacgaca gcttcaactt caagttcagc ctgcacccca acgacctggt ggaggtgatc     2460

accaagaagg cccgcatgtt cggctacttc gccagctgcc accgcggcac cggcaacatc     2520

aacatccgca tccacgacct ggaccacaag atcggcaaga acggcatcct ggagggcatc     2580

ggcgtgaaga ccgccctgag cttccagaag taccagatcg acgagctggg caaggagatc     2640

cgcccctgcc gcctgaagaa gcgccctcct gtgcgctaa                            2679


<210>  2
<211>  3000
<212>  DNA
<213>  Neisseria meningitidis

<400>  2
atggccgcct tcaagcccaa ccccatcaac tacatcctgg gcctggccat cggcatcgcc       60

agcgtgggct gggccatggt ggagatcgac gaggacgaga accccatctg cctgatcgac      120

ctgggtgtgc gcgtgttcga gcgcgctgag gtgcccaaga ctggtgacag tctggctatg      180

gctcgccggc ttgctcgctc tgttcggcgc cttactcgcc ggcgcgctca ccgccttctg      240

cgcgctcgcc gcctgctgaa gcgcgagggt gtgctgcagg ctgccgactt cgacgagaac      300

ggcctgatca agagcctgcc caacactcct tggcagctgc gcgctgccgc tctggaccgc      360

aagctgactc ctctggagtg gagcgccgtg ctgctgcacc tgatcaagca ccgcggctac      420

ctgagccagc gcaagaacga gggcgagacc gccgacaagg agctgggtgc tctgctgaag      480

ggcgtggccg acaacgccca cgccctgcag actggtgact tccgcactcc tgctgagctg      540

gccctgaaca agttcgagaa ggagagcggc cacatccgca accagcgcgg cgactacagc      600

cacaccttca gccgcaagga cctgcaggcc gagctgatcc tgctgttcga gaagcagaag      660

gagttcggca acccccacgt gagcggcggc ctgaaggagg gcatcgagac cctgctgatg      720

acccagcgcc ccgccctgag cggcgacgcc gtgcagaaga tgctgggcca ctgcaccttc      780

gagccagccg agcccaaggc cgccaagaac acctacaccg ccgagcgctt catctggctg      840

accaagctga acaacctgcg catcctggag cagggcagcg agcgccccct gaccgacacc      900

gagcgcgcca ccctgatgga cgagccctac cgcaagagca agctgaccta cgcccaggcc      960

cgcaagctgc tgggtctgga ggacaccgcc ttcttcaagg gcctgcgcta cggcaaggac     1020

aacgccgagg ccagcaccct gatggagatg aaggcctacc acgccatcag ccgcgccctg     1080

gagaaggagg gcctgaagga caagaagagt cctctgaacc tgagccccga gctgcaggac     1140

gagatcggca ccgccttcag cctgttcaag accgacgagg acatcaccgg ccgcctgaag     1200

gaccgcatcc agcccgagat cctggaggcc ctgctgaagc acatcagctt cgacaagttc     1260

gtgcagatca gcctgaaggc cctgcgccgc atcgtgcccc tgatggagca gggcaagcgc     1320

tacgacgagg cctgcgccga gatctacggc gaccactacg gcaagaagaa caccgaggag     1380

aagatctacc tgcctcctat ccccgccgac gagatccgca accccgtggt gctgcgcgcc     1440

ctgagccagg cccgcaaggt gatcaacggc gtggtgcgcc gctacggcag ccccgcccgc     1500

atccacatcg agaccgcccg cgaggtgggc aagagcttca aggaccgcaa ggagatcgag     1560

aagcgccagg aggagaaccg caaggaccgc gagaaggccg ccgccaagtt ccgcgagtac     1620

ttccccaact tcgtgggcga gcccaagagc aaggacatcc tgaagctgcg cctgtacgag     1680

cagcagcacg gcaagtgctc cggcggcggt tcgcagaagt tcgacgagga cggcttcaag     1740

gagcgcaacc tgaacgacac ccgctacgtg aaccgcttcc tgtgccagtt cgtggccgac     1800

cgcatgcgcc tgaccggcaa gggcaagaag cgcgtgttcg ccagcaacgg ccagatcacc     1860

aacctgctgc gcggcttctg gggcctgcgc aaggtgcgcg ccgagaacga ccgccaccac     1920

gccctggacg ccgtggtggt ggcctgcagc accgtggcca tgcagcagaa gatcacccgc     1980

ttcgtgcgct acaaggagat gaacgccttc gacggtaaaa ccatcgacaa ggagaccggc     2040

gaggtgctgc accagaagac ccacttcccc cagccctggg agttcttcgc ccaggaggtg     2100

atgatccgcg tgttcggcaa gcccgacggc aagcccgagt tcgaggaggc cgacaccccc     2160

gagaagctgc gcaccctgct ggccgagaag ctgagcagcc gccctgaggc cgtgcacgag     2220

tacgtgactc ctctgttcgt gagccgcgcc cccaaccgca agatgagcgg tcagggtcac     2280

atggagaccg tgaagagcgc caagcgcctg gacgagggcg tgagcgtgct gcgcgtgccc     2340

ctgacccagc tgaagctgaa ggacctggag aagatggtga accgcgagcg cgagcccaag     2400

ctgtacgagg ccctgaaggc ccgcctggag gcccacaagg acgaccccgc caaggccttc     2460

gccgagccct tctacaagta cgacaaggcc ggcaaccgca cccagcaggt gaaggccgtg     2520

cgcgtggagc aggtgcagaa gaccggcgtg tgggtgcgca accacaacgg catcgccgac     2580

aacgccacca tggtgcgcgt ggacgtgttc gagaagggcg acaagtacta cctggtgccc     2640

atctacagct ggcaggtggc caagggcatc ctgcccgacc gcgccgtggt gcagggcaag     2700

gacgaggagg actggcagct gatcgacgac agcttcaact tcaagttcag cctgcacccc     2760

aacgacctgg tggaggtgat caccaagaag gcccgcatgt tcggctactt cgccagctgc     2820

caccgcggca ccggcaacat caacatccgc atccacgacc tggaccacaa gatcggcaag     2880

aacggcatcc tggagggcat cggcgtgaag accgccctga gcttccagaa gtaccagatc     2940

gacgagctgg gcaaggagat ccgcccctgc cgcctgaaga agcgccctcc tgtgcgctaa     3000


<210>  3
<211>  5064
<212>  DNA
<213>  Neisseria meningitidis

<400>  3
agctctcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat       60

gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc      120

gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac      180

acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg      240

caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag      300

cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc      360

gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc      420

gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga      480

tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa      540

tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc      600

cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga      660

caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc      720

atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc      780

ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt      840

ctcttgatca gatcttgatc ccctgcgcca tcagatcctt ggcggcaaga aagccatcca      900

gtttactttg cagggcttcc caaccttacc agagggcgcc ccagctggca attccgacgt      960

ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt     1020

tcgtcttcac ctcgagggga caatgaaaac gttagtcatg gcgcgccttg acggctagct     1080

cagtcctagg tacagtgcta gcttaatgct cgcacatagc agaactttaa aagtattcgc     1140

catgttgtag ctccctttct catttcgcag tgctacaatc cgccgctatg gtcccacgta     1200

gagcatacgg aaaaaaaagt caaaagcctc cgaccggagg tcggccttac ttgctagcag     1260

agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttaa tttgatgcct     1320

ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttcg caacgttcaa     1380

atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa     1440

acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcctgg cagttcccta     1500

ctctcgcatg gggagacccc acactaccat cggcgctacg gcgtttcact tctgagttcg     1560

gcatggggtc aggtgggacc accgcgctac tgccgccagg caaattctgt tttatcagac     1620

cgcttctgcg ttctgattta atctgtatca ggctgaaaat cttctctcat ccgccaaaac     1680

agccccgtag aaaaagggac gttgatcggc acgtaagagg ttccacgata aatatctaac     1740

accgtgcgtg ttgactattt tacctctggc ggtgataatg gttgcatgta ctagaattct     1800

ttaactttaa gaaggagata tacatatgaa tcccctatgg cgaatacttt taaagtctcg     1860

taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt     1920

taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact     1980

tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac     2040

tactttcggt tatggtctaa aatgctttgc tagataccca gatcatatga aacggcatga     2100

ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga     2160

tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag     2220

aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga     2280

atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa     2340

agttaacttc aaaattagac acaacattga agatggaagc gttcaactag cagaccatta     2400

tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc     2460

ctatcaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga     2520

gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat aagcttaacc     2580

gaagcgtttg atagttgata tcctttgcct gcggccgcaa ctagaggcat caaataaaac     2640

gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc     2700

tcctgagtag gacaaatccg ccgccctaga cctagggtac gggttttgct gcccgcaaac     2760

gggctgttct ggtgttgcta gtttgttatc agaatcgcag atccggcttc aggtttgccg     2820

gctgaaagcg ctatttcttc cagaattgcc atgatttttt ccccacggga ggcgtcactg     2880

gctcccgtgt tgtcggcagc tttgattcga taagcagcat cgcctgtttc aggctgtcta     2940

tgtgtgactg ttgagctgta acaagttgtc tcaggtgttc aatttcatgt tctagttgct     3000

ttgttttact ggtttcacct gttctattag gtgttacatg ctgttcatct gttacattgt     3060

cgatctgttc atggtgaaca gctttaaatg caccaaaaac tcgtaaaagc tctgatgtat     3120

ctatcttttt tacaccgttt tcatctgtgc atatggacag ttttcccttt gatatctaac     3180

ggtgaacagt tgttctactt ttgtttgtta gtcttgatgc ttcactgata gatacaagag     3240

ccataagaac ctcagatcct tccgtattta gccagtatgt tctctagtgt ggttcgttgt     3300

ttttgcgtga gccatgagaa cgaaccattg agatcatgct tactttgcat gtcactcaaa     3360

aattttgcct caaaactggt gagctgaatt tttgcagtta aagcatcgtg tagtgttttt     3420

cttagtccgt tacgtaggta ggaatctgat gtaatggttg ttggtatttt gtcaccattc     3480

atttttatct ggttgttctc aagttcggtt acgagatcca tttgtctatc tagttcaact     3540

tggaaaatca acgtatcagt cgggcggcct cgcttatcaa ccaccaattt catattgctg     3600

taagtgttta aatctttact tattggtttc aaaacccatt ggttaagcct tttaaactca     3660

tggtagttat tttcaagcat taacatgaac ttaaattcat caaggctaat ctctatattt     3720

gccttgtgag ttttcttttg tgttagttct tttaataacc actcataaat cctcatagag     3780

tatttgtttt caaaagactt aacatgttcc agattatatt ttatgaattt ttttaactgg     3840

aaaagataag gcaatatctc ttcactaaaa actaattcta atttttcgct tgagaacttg     3900

gcatagtttg tccactggaa aatctcaaag cctttaacca aaggattcct gatttccaca     3960

gttctcgtca tcagctctct ggttgcttta gctaatacac cataagcatt ttccctactg     4020

atgttcatca tctgagcgta ttggttataa gtgaacgata ccgtccgttc tttccttgta     4080

gggttttcaa tcgtggggtt gagtagtgcc acacagcata aaattagctt ggtttcatgc     4140

tccgttaagt catagcgact aatcgctagt tcatttgctt tgaaaacaac taattcagac     4200

atacatctca attggtctag gtgattttaa tcactatacc aattgagatg ggctagtcaa     4260

tgataattac tagtcctttt cctttgagtt gtgggtatct gtaaattctg ctagaccttt     4320

gctggaaaac ttgtaaattc tgctagaccc tctgtaaatt ccgctagacc tttgtgtgtt     4380

ttttttgttt atattcaagt ggttataatt tatagaataa agaaagaata aaaaaagata     4440

aaaagaatag atcccagccc tgtgtataac tcactacttt agtcagttcc gcagtattac     4500

aaaaggatgt cgcaaacgct gtttgctcct ctacaaaaca gaccttaaaa ccctaaaggc     4560

ttaagtagca ccctcgcaag ctcgggcaaa tcgctgaata ttccttttgt ctccgaccat     4620

caggcacctg agtcgctgtc tttttcgtga cattcagttc gctgcgctca cggctctggc     4680

agtgaatggg ggtaaatggc actacaggcg ccttttatgg attcatgcaa ggaaactacc     4740

cataatacaa gaaaagcccg tcacgggctt ctcagggcgt tttatggcgg gtctgctatg     4800

tggtgctatc tgactttttg ctgttcagca gttcctgccc tctgattttc cagtctgacc     4860

acttcggatt atcccgtgac aggtcattca gactggctaa tgcacccagt aaggcagcgg     4920

tatcatcaac aggcttaccc gtcttactgt ccctagtgct tggattctca ccaataaaaa     4980

acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg     5040

gatctatcaa caggagtcca agcg                                            5064


