                         SEQUENCE LISTING

<110>  WASHINGTON UNIVERSITY
       Arul, VARMAN M.
       Yi, YU
       Yinjie, TANG J.
 
<120>  METHODS OF PRODUCING D-LACTIC ACID IN CYANOBACTERIA

<130>  047563-485266

<150>  US 61/906,701
<151>  2013-11-20

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  367
<212>  PRT
<213>  Bacillus coagulans

<400>  1

Met Thr Lys Ile Ile Thr Ser Pro Ser Lys Phe Ile Gln Gly Pro Asp 
1               5                   10                  15      


Glu Leu Ser Arg Leu Ser Ala Tyr Thr Glu Arg Leu Gly Lys Lys Ala 
            20                  25                  30          


Phe Ile Ile Ala Asp Asp Phe Val Thr Gly Leu Val Gly Lys Thr Val 
        35                  40                  45              


Glu Glu Ser Tyr Ala Gly Lys Glu Thr Gly Tyr Gln Met Ala Leu Phe 
    50                  55                  60                  


Gly Gly Glu Cys Ser Lys Pro Glu Ile Glu Arg Leu Cys Glu Met Ser 
65                  70                  75                  80  


Lys Ser Glu Glu Ala Asp Val Val Val Gly Ile Gly Gly Gly Lys Thr 
                85                  90                  95      


Leu Asp Thr Ala Lys Ala Val Gly Tyr Tyr Asn Asn Ile Pro Val Ile 
            100                 105                 110         


Val Ala Pro Thr Ile Ala Ser Thr Asn Ala Pro Thr Ser Ala Leu Ser 
        115                 120                 125             


Val Ile Tyr Lys Glu Asn Gly Glu Phe Glu Glu Tyr Leu Met Leu Pro 
    130                 135                 140                 


Leu Asn Pro Thr Phe Val Ile Met Asp Thr Lys Val Ile Ala Ser Ala 
145                 150                 155                 160 


Pro Ala Arg Leu Leu Val Ser Gly Met Gly Asp Ala Leu Ala Thr Tyr 
                165                 170                 175     


Phe Glu Ala Arg Ala Thr Lys Arg Ala Asn Lys Thr Thr Met Ala Gly 
            180                 185                 190         


Gly Arg Val Thr Glu Ala Ala Ile Ala Leu Ala Lys Leu Cys Tyr Asp 
        195                 200                 205             


Thr Gln Ile Ser Glu Gly Leu Lys Ala Lys Leu Ala Ala Glu Lys His 
    210                 215                 220                 


Leu Val Thr Glu Ala Val Glu Lys Ile Ile Glu Ala Asn Thr Tyr Leu 
225                 230                 235                 240 


Ser Gly Ile Gly Ser Glu Ser Gly Gly Leu Ala Ala Ala His Ala Ile 
                245                 250                 255     


His Asn Gly Leu Thr Val Leu Glu Glu Thr His His Met Tyr His Gly 
            260                 265                 270         


Glu Lys Val Ala Phe Gly Thr Leu Ala Gln Leu Ile Leu Glu Asp Ala 
        275                 280                 285             


Pro Lys Ala Glu Ile Glu Glu Val Val Ser Phe Cys Leu Ser Val Gly 
    290                 295                 300                 


Leu Pro Val Thr Leu Gly Asp Leu Gly Val Lys Glu Leu Asn Glu Glu 
305                 310                 315                 320 


Lys Leu Arg Lys Val Ala Glu Leu Ser Cys Ala Glu Gly Glu Thr Ile 
                325                 330                 335     


Tyr Asn Met Pro Phe Glu Val Thr Pro Asp Leu Val Tyr Ala Ala Ile 
            340                 345                 350         


Val Thr Ala Asp Ser Val Gly Arg Tyr Tyr Lys Glu Lys Trp Ala 
        355                 360                 365         


<210>  2
<211>  1104
<212>  DNA
<213>  Bacillus coagulans

<400>  2
atgacgaaaa tcattacctc tccaagcaag tttatacaag gccccgatga attgtccagg       60

ctttcggcgt atacggaaag gcttggcaaa aaagcattta ttattgcgga tgattttgtc      120

accggccttg tcggcaaaac ggttgaagaa agctatgccg gcaaagaaac ggggtatcaa      180

atggcattat tcggtggtga gtgttctaaa ccggaaatcg aacggctttg tgaaatgagc      240

aaatccgagg aagccgatgt cgttgtcgga atcggcggcg gaaaaacatt ggataccgca      300

aaagcagtcg ggtattacaa taacattccg gtgattgtcg cgccgaccat cgcttccacc      360

aatgccccga caagcgccct gtctgttatt tacaaagaga acggcgagtt tgaagaatac      420

ttgatgctgc cgctgaaccc gacttttgtc attatggata cgaaagtgat tgcctctgcc      480

cctgcccgcc tgctcgtttc cggcatggga gatgcgcttg caacgtattt tgaagcgcgc      540

gccactaagc gggcaaataa aacgacgatg gcaggcgggc gtgttacgga agcggcgatc      600

gcgcttgcaa aactttgtta tgacacgcaa atttcggaag gtttaaaagc aaaactggca      660

gcggaaaaac atcttgttac ggaagcagtg gaaaaaatca ttgaagcgaa tacgtatctg      720

agcggaatcg gttctgaaag cggcggcctt gctgcggcac atgcgatcca taatgggctt      780

accgtgctcg aagaaaccca tcatatgtac cacggcgaaa aagtggcatt cggtaccctc      840

gcccagctga ttttggaaga tgcgccgaaa gcggaaattg aagaggtggt ctccttctgc      900

ctgagtgtcg gacttcccgt cacgctcggg gatttgggcg tgaaagaact gaatgaggaa      960

aagctccgaa aagtggctga actttcctgt gcggaaggcg aaacgattta taacatgccg     1020

tttgaagtca cgcctgacct tgtgtacgca gcaatcgtta ccgctgattc cgtcgggcgg     1080

tattataagg aaaaatgggc atga                                            1104


<210>  3
<211>  1104
<212>  DNA
<213>  Bacillus coagulans

<400>  3
atgaccaaaa tcatcacctc cccctccaaa ttcatccagg gccccgacga gttgtcccgg       60

ttgagtgcct ataccgaacg gttgggcaag aaggccttta tcatcgctga cgacttcgtg      120

accggcttgg tgggtaagac tgtggaggag tcctatgccg gcaaagaaac cggttaccag      180

atggccttgt ttggcggcga atgcagtaag cctgagatcg agcgctcttg cgagatgtcc      240

aaatccgaag aagccgacgt tgtggtgggc atcggcggtg gcaaaacctt agacactgcc      300

aaggccgtgg gctactacaa taacattccc gtgatcgtgg cccccactat tgcttccacc      360

aatgccccca cctccgcctt gagtgtgatc tataaggaga acggcgaatt cgaggaatac      420

ttgatgttgc ctttgaaccc tacctttgtg atcatggaca ctaaggtgat cgcctccgct      480

cctgcccgct tattggtttc cggcatgggc gacgccttgg ccacttactt tgaagcccgc      540

gccactaagc gggccaataa gaccaccatg gctggtggcc gcgttactga ggccgccatc      600

gccttggcca agttgtgcta cgacacccag atcagtgagg gcttgaaggc caagttagcc      660

gccgagaaac acttggtgac cgaggccgtt gagaagatca tcgaggccaa cacttacttg      720

tccggtattg gctccgaaag tggcggctta gccgccgctc acgccattca caacggcttg      780

accgtgttgg aggagaccca ccacatgtac cacggcgaga aggtggcttt cggcactttg      840

gcccagttga tcttggagga tgcccccaag gccgagattg aggaggtggt gtccttctgt      900

ttgtccgtgg gcttgcctgt taccttgggc gacttgggcg tgaaggaatt gaacgaggaa      960

aaattgcgga aggtggccga attgtcctgc gccgagggcg aaaccatcta caacatgccc     1020

tttgaggtga cccccgattt ggtgtacgcc gccatcgtta ccgctgactc cgtgggccgc     1080

tattacaagg aaaagtgggc ctaa                                            1104


<210>  4
<211>  1395
<212>  DNA
<213>  Pseudomonas aeruginosa

<400>  4
atggctgtct acaactacga cgtggtgatt ttgggcacgg gtccggcggg cgaaggggcg       60

gcgatgaacg cctccaagta cggacgcaag ctggcggtag tcgacagccg tcgcgtggtc      120

ggaggcaact gcacgcacct cggcaccatt ccttccaagg ccctgcgcca ctcggtgaag      180

cagatcatcg agttcaacac caacccgatg ttccgccaga tcggcgagcc gcgctggttc      240

tccttccccg acgtgctgaa gagcgccgac cgggtgatct ccaagcaggt cgcttcgcgt      300

accggctact acgcgcgcaa tcgcatcgac atgttcaccg gcaccgccag cttcgtcgac      360

gaacgcaccg tcgaagtggt gacgccgagc ggcgcggtgg agcgcctggt cgccgaccag      420

ttcgtgatcg ccaccggctc gcgcccgtat cgtccttcgg acatcaattt caaccacccg      480

cgggtctacg acagcgacac catcctgtcc ctgagccaca ccccgcgccg gctgatcatc      540

tacggagccg gggtgatcgg ctgcgaatac gcgtcgatct tcagcggcct gggcgtgctg      600

gtggacctga tcgatacgcg cgaccagttg ctcagcttcc tcgatgacga gatttccgat      660

gcgctgagct accacctgcg caacaacaac gtgctgatcc gccacaacga ggagtacgag      720

cgcgtcgagg ggctggacaa cggtgtcatc ctgcacctga agtcgggcaa gaagatcaag      780

gcggacgcgc tgctctggtg caacggccgg accggcaaca ccgacaagct cggcctggag      840

aacgtcggca tcaaggtcaa cagccgtggc cagatcgagg tggacgagaa ctaccgcact      900

tcggtgagca acatcttcgc cgctggcgac gtgatcggct ggccgagcct ggccagcgct      960

gcctacgacc agggccgctc ggccgccggc aacatcgtcg agagcgacag ctggcgcttc     1020

gtcaacgatg tgccgaccgg catctacacc attccggaga tcagctcgat cggcaagaac     1080

gaaagcgaac tgaccgcggc gaagattccc tacgaagtgg gcaaggcgtt cttcaagggc     1140

atggcccggg cgcagatttc caacgagccg gtgggcatgc tgaagatcct gttccatcgc     1200

gagaccctgg agatcctcgg cgtgcactgc ttcggcgacc aggcttcgga aatcgtccac     1260

atcggccagg cgatcatgaa ccagccgggc gagctgaata ccctgaagta cttcgtcaac     1320

accaccttca actacccgac catggcggaa gcctatcggg tagcggcgtt cgacggcctc     1380

aaccggcttt tttga                                                      1395


<210>  5
<211>  464
<212>  PRT
<213>  Pseudomonas aeruginosa

<400>  5

Met Ala Val Tyr Asn Tyr Asp Val Val Ile Leu Gly Thr Gly Pro Ala 
1               5                   10                  15      


Gly Glu Gly Ala Ala Met Asn Ala Ser Lys Tyr Gly Arg Lys Leu Ala 
            20                  25                  30          


Val Val Asp Ser Arg Arg Val Val Gly Gly Asn Cys Thr His Leu Gly 
        35                  40                  45              


Thr Ile Pro Ser Lys Ala Leu Arg His Ser Val Lys Gln Ile Ile Glu 
    50                  55                  60                  


Phe Asn Thr Asn Pro Met Phe Arg Gln Ile Gly Glu Pro Arg Trp Phe 
65                  70                  75                  80  


Ser Phe Pro Asp Val Leu Lys Ser Ala Asp Arg Val Ile Ser Lys Gln 
                85                  90                  95      


Val Ala Ser Arg Thr Gly Tyr Tyr Ala Arg Asn Arg Ile Asp Met Phe 
            100                 105                 110         


Thr Gly Thr Ala Ser Phe Val Asp Glu Arg Thr Val Glu Val Val Thr 
        115                 120                 125             


Pro Ser Gly Ala Val Glu Arg Leu Val Ala Asp Gln Phe Val Ile Ala 
    130                 135                 140                 


Thr Gly Ser Arg Pro Tyr Arg Pro Ser Asp Ile Asn Phe Asn His Pro 
145                 150                 155                 160 


Arg Val Tyr Asp Ser Asp Thr Ile Leu Ser Leu Ser His Thr Pro Arg 
                165                 170                 175     


Arg Leu Ile Ile Tyr Gly Ala Gly Val Ile Gly Cys Glu Tyr Ala Ser 
            180                 185                 190         


Ile Phe Ser Gly Leu Gly Val Leu Val Asp Leu Ile Asp Thr Arg Asp 
        195                 200                 205             


Gln Leu Leu Ser Phe Leu Asp Asp Glu Ile Ser Asp Ala Leu Ser Tyr 
    210                 215                 220                 


His Leu Arg Asn Asn Asn Val Leu Ile Arg His Asn Glu Glu Tyr Glu 
225                 230                 235                 240 


Arg Val Glu Gly Leu Asp Asn Gly Val Ile Leu His Leu Lys Ser Gly 
                245                 250                 255     


Lys Lys Ile Lys Ala Asp Ala Leu Leu Trp Cys Asn Gly Arg Thr Gly 
            260                 265                 270         


Asn Thr Asp Lys Leu Gly Leu Glu Asn Val Gly Ile Lys Val Asn Ser 
        275                 280                 285             


Arg Gly Gln Ile Glu Val Asp Glu Asn Tyr Arg Thr Ser Val Ser Asn 
    290                 295                 300                 


Ile Phe Ala Ala Gly Asp Val Ile Gly Trp Pro Ser Leu Ala Ser Ala 
305                 310                 315                 320 


Ala Tyr Asp Gln Gly Arg Ser Ala Ala Gly Asn Ile Val Glu Ser Asp 
                325                 330                 335     


Ser Trp Arg Phe Val Asn Asp Val Pro Thr Gly Ile Tyr Thr Ile Pro 
            340                 345                 350         


Glu Ile Ser Ser Ile Gly Lys Asn Glu Ser Glu Leu Thr Ala Ala Lys 
        355                 360                 365             


Ile Pro Tyr Glu Val Gly Lys Ala Phe Phe Lys Gly Met Ala Arg Ala 
    370                 375                 380                 


Gln Ile Ser Asn Glu Pro Val Gly Met Leu Lys Ile Leu Phe His Arg 
385                 390                 395                 400 


Glu Thr Leu Glu Ile Leu Gly Val His Cys Phe Gly Asp Gln Ala Ser 
                405                 410                 415     


Glu Ile Val His Ile Gly Gln Ala Ile Met Asn Gln Pro Gly Glu Leu 
            420                 425                 430         


Asn Thr Leu Lys Tyr Phe Val Asn Thr Thr Phe Asn Tyr Pro Thr Met 
        435                 440                 445             


Ala Glu Ala Tyr Arg Val Ala Ala Phe Asp Gly Leu Asn Arg Leu Phe 
    450                 455                 460                 


<210>  6
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHESIZED

<400>  6
ggatccttga caattaatca tccggctcg                                         29


<210>  7
<211>  124
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHESIZED

<400>  7
ggatccttga caattaatca tccggctcgt ataatgtgtg gaattgtgag cggataacaa       60

tttcacacag gagatataat catatgacga aaatcattac ctctccaagc aagtttatac      120

aagg                                                                   124


<210>  8
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHESIZED

<400>  8
atgacgaaaa tcattacctc tccaag                                            26


<210>  9
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHESIZED

<400>  9
gctagctcat gcccattttt ccttataata ccgcccg                                37


<210>  10
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHESIZED

<400>  10
ttaggcccac ttttccttgt aatagc                                            26


<210>  11
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHESIZED

<400>  11
cctaagctag cggaggacta gcatgg                                            26


<210>  12
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHESIZED

<400>  12
gctagcggta cctcaaaaaa gccgg                                             25


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHESIZED

<400>  13
cccgaagtgg cgagcccgat                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHESIZED

<400>  14
ttgatgttgc ctttgaaccc                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHESIZED

<400>  15
atggatacga aagtgattgc                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHESIZED

<400>  16
gagctaccac ctgcgcaaca                                                   20


<210>  17
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHESIZED

<400>  17
gcgcgactcc ccgtctttga ctatcctttt taggatgggg ca                          42


<210>  18
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHESIZED

<400>  18
taccggaaca ggaccaagcc tt                                                22


