                         SEQUENCE LISTING

<110>  Regeneron Pharmaceuticals, Inc.
       McWhirter, John
       Gurer, Cagan
       Macdonald, Lynn
       Murphy, Andrew J.
 
<120>  NON-HUMAN ANIMALS HAVING A HUMANIZED B-CELL ACTIVATING FACTOR 
       GENE

<130>  31015 (6800)

<150>  61/905,983
<151>  2013-11-19

<160>  19    

<170>  PatentIn version 3.5

<210>  1
<211>  1710
<212>  DNA
<213>  Mus musculus

<400>  1
ggcacgaggc agattgagca atccatggaa ggccagagcc agagaaccta cttcagggta       60

gcaaaagatg cagaagaaag tcaggagagc gctcctgggg gaacccagcc ctgccatgct      120

ctgagggcag tctcccagga cacagatgac aggaaatgac ccacccctgt ggtcacttac      180

tccaaaggcc tagaccttca aagtgctcct cgtggaatgg atgagtctgc aaagaccctg      240

ccaccaccgt gcctctgttt ttgctccgag aaaggagaag atatgaaagt gggatatgat      300

cccatcactc cgcagaagga ggagggtgcc tggtttggga tctgcaggga tggaaggctg      360

ctggctgcta ccctcctgct ggccctgttg tccagcagtt tcacagcgat gtccttgtac      420

cagttggctg ccttgcaagc agacctgatg aacctgcgca tggagctgca gagctaccga      480

ggttcagcaa caccagccgc cgcgggtgct ccagagttga ccgctggagt caaactcctg      540

acaccggcag ctcctcgacc ccacaactcc agccgcggcc acaggaacag acgcgctttc      600

cagggaccag aggaaacaga acaagatgta gacctctcag ctcctcctgc accatgcctg      660

cctggatgcc gccattctca acatgatgat aatggaatga acctcagaaa catcattcaa      720

gactgtctgc agctgattgc agacagcgac acgccgacta tacgaaaagg aacttacaca      780

tttgttccat ggcttctcag ctttaaaaga ggaaatgcct tggaggagaa agagaacaaa      840

atagtggtga ggcaaacagg ctatttcttc atctacagcc aggttctata cacggacccc      900

atctttgcta tgggtcatgt catccagagg aagaaagtac acgtctttgg ggacgagctg      960

agcctggtga ccctgttccg atgtattcag aatatgccca aaacactgcc caacaattcc     1020

tgctactcgg ctggcatcgc gaggctggaa gaaggagatg agattcagct tgcaattcct     1080

cgggagaatg cacagatttc acgcaacgga gacgacacct tctttggtgc cctaaaactg     1140

ctgtaactca cttgctggag tgcgtgatcc ccttccctcg tcttctctgt acctccgagg     1200

gagaaacaga cgactggaaa aactaaaaga tggggaaagc cgtcagcgaa agttttctcg     1260

tgacccgttg aatctgatcc aaaccaggaa atataacaga cagccacaac cgaagtgtgc     1320

catgtgagtt atgagaaacg gagcccgcgc tcagaaagac cggatgagga agaccgtttt     1380

ctccagtcct ttgccaacac gcaccgcaac cttgcttttt gccttgggtg acacatgttc     1440

agaatgcagg gagatttcct tgttttgcga tttgccatga gaagagggcc cacaactgca     1500

ggtcactgaa gcattcacgc taagtctcag gatttactct cccttctcat gctaagtaca     1560

cacacgctct tttccaggta atactatggg atactatgga aaggttgttt gtttttaaat     1620

ctagaagtct tgaactggca atagacaaaa atccttataa attcaagtgt aaaataaact     1680

taattaaaaa ggtttaagtg tgaaaaaaaa                                      1710


<210>  2
<211>  309
<212>  PRT
<213>  Mus musculus

<400>  2

Met Asp Glu Ser Ala Lys Thr Leu Pro Pro Pro Cys Leu Cys Phe Cys 
1               5                   10                  15      


Ser Glu Lys Gly Glu Asp Met Lys Val Gly Tyr Asp Pro Ile Thr Pro 
            20                  25                  30          


Gln Lys Glu Glu Gly Ala Trp Phe Gly Ile Cys Arg Asp Gly Arg Leu 
        35                  40                  45              


Leu Ala Ala Thr Leu Leu Leu Ala Leu Leu Ser Ser Ser Phe Thr Ala 
    50                  55                  60                  


Met Ser Leu Tyr Gln Leu Ala Ala Leu Gln Ala Asp Leu Met Asn Leu 
65                  70                  75                  80  


Arg Met Glu Leu Gln Ser Tyr Arg Gly Ser Ala Thr Pro Ala Ala Ala 
                85                  90                  95      


Gly Ala Pro Glu Leu Thr Ala Gly Val Lys Leu Leu Thr Pro Ala Ala 
            100                 105                 110         


Pro Arg Pro His Asn Ser Ser Arg Gly His Arg Asn Arg Arg Ala Phe 
        115                 120                 125             


Gln Gly Pro Glu Glu Thr Glu Gln Asp Val Asp Leu Ser Ala Pro Pro 
    130                 135                 140                 


Ala Pro Cys Leu Pro Gly Cys Arg His Ser Gln His Asp Asp Asn Gly 
145                 150                 155                 160 


Met Asn Leu Arg Asn Ile Ile Gln Asp Cys Leu Gln Leu Ile Ala Asp 
                165                 170                 175     


Ser Asp Thr Pro Thr Ile Arg Lys Gly Thr Tyr Thr Phe Val Pro Trp 
            180                 185                 190         


Leu Leu Ser Phe Lys Arg Gly Asn Ala Leu Glu Glu Lys Glu Asn Lys 
        195                 200                 205             


Ile Val Val Arg Gln Thr Gly Tyr Phe Phe Ile Tyr Ser Gln Val Leu 
    210                 215                 220                 


Tyr Thr Asp Pro Ile Phe Ala Met Gly His Val Ile Gln Arg Lys Lys 
225                 230                 235                 240 


Val His Val Phe Gly Asp Glu Leu Ser Leu Val Thr Leu Phe Arg Cys 
                245                 250                 255     


Ile Gln Asn Met Pro Lys Thr Leu Pro Asn Asn Ser Cys Tyr Ser Ala 
            260                 265                 270         


Gly Ile Ala Arg Leu Glu Glu Gly Asp Glu Ile Gln Leu Ala Ile Pro 
        275                 280                 285             


Arg Glu Asn Ala Gln Ile Ser Arg Asn Gly Asp Asp Thr Phe Phe Gly 
    290                 295                 300                 


Ala Leu Lys Leu Leu 
305                 


<210>  3
<211>  290
<212>  PRT
<213>  Mus musculus

<400>  3

Met Asp Glu Ser Ala Lys Thr Leu Pro Pro Pro Cys Leu Cys Phe Cys 
1               5                   10                  15      


Ser Glu Lys Gly Glu Asp Met Lys Val Gly Tyr Asp Pro Ile Thr Pro 
            20                  25                  30          


Gln Lys Glu Glu Gly Ala Trp Phe Gly Ile Cys Arg Asp Gly Arg Leu 
        35                  40                  45              


Leu Ala Ala Thr Leu Leu Leu Ala Leu Leu Ser Ser Ser Phe Thr Ala 
    50                  55                  60                  


Met Ser Leu Tyr Gln Leu Ala Ala Leu Gln Ala Asp Leu Met Asn Leu 
65                  70                  75                  80  


Arg Met Glu Leu Gln Ser Tyr Arg Gly Ser Ala Thr Pro Ala Ala Ala 
                85                  90                  95      


Gly Ala Pro Glu Leu Thr Ala Gly Val Lys Leu Leu Thr Pro Ala Ala 
            100                 105                 110         


Pro Arg Pro His Asn Ser Ser Arg Gly His Arg Asn Arg Arg Ala Phe 
        115                 120                 125             


Gln Gly Pro Glu Glu Thr Glu Gln Asp Val Asp Leu Ser Ala Pro Pro 
    130                 135                 140                 


Ala Pro Cys Leu Pro Gly Cys Arg His Ser Gln His Asp Asp Asn Gly 
145                 150                 155                 160 


Met Asn Leu Arg Asn Arg Thr Tyr Thr Phe Val Pro Trp Leu Leu Ser 
                165                 170                 175     


Phe Lys Arg Gly Asn Ala Leu Glu Glu Lys Glu Asn Lys Ile Val Val 
            180                 185                 190         


Arg Gln Thr Gly Tyr Phe Phe Ile Tyr Ser Gln Val Leu Tyr Thr Asp 
        195                 200                 205             


Pro Ile Phe Ala Met Gly His Val Ile Gln Arg Lys Lys Val His Val 
    210                 215                 220                 


Phe Gly Asp Glu Leu Ser Leu Val Thr Leu Phe Arg Cys Ile Gln Asn 
225                 230                 235                 240 


Met Pro Lys Thr Leu Pro Asn Asn Ser Cys Tyr Ser Ala Gly Ile Ala 
                245                 250                 255     


Arg Leu Glu Glu Gly Asp Glu Ile Gln Leu Ala Ile Pro Arg Glu Asn 
            260                 265                 270         


Ala Gln Ile Ser Arg Asn Gly Asp Asp Thr Phe Phe Gly Ala Leu Lys 
        275                 280                 285             


Leu Leu 
    290 


<210>  4
<211>  2675
<212>  DNA
<213>  Homo sapiens

<400>  4
gaaattctta caaaaactga aagtgaaatg aggaagacag attgagcaat ccaatcggag       60

ggtaaatgcc agcaaaccta ctgtacagta ggggtagaga tgcagaaagg cagaaaggag      120

aaaattcagg ataactctcc tgaggggtga gccaagccct gccatgtagt gcacgcagga      180

catcaacaaa cacagataac aggaaatgat ccattccctg tggtcactta ttctaaaggc      240

cccaaccttc aaagttcaag tagtgatatg gatgactcca cagaaaggga gcagtcacgc      300

cttacttctt gccttaagaa aagagaagaa atgaaactga aggagtgtgt ttccatcctc      360

ccacggaagg aaagcccctc tgtccgatcc tccaaagacg gaaagctgct ggctgcaacc      420

ttgctgctgg cactgctgtc ttgctgcctc acggtggtgt ctttctacca ggtggccgcc      480

ctgcaagggg acctggccag cctccgggca gagctgcagg gccaccacgc ggagaagctg      540

ccagcaggag caggagcccc caaggccggc ctggaggaag ctccagctgt caccgcggga      600

ctgaaaatct ttgaaccacc agctccagga gaaggcaact ccagtcagaa cagcagaaat      660

aagcgtgccg ttcagggtcc agaagaaaca gtcactcaag actgcttgca actgattgca      720

gacagtgaaa caccaactat acaaaaagga tcttacacat ttgttccatg gcttctcagc      780

tttaaaaggg gaagtgccct agaagaaaaa gagaataaaa tattggtcaa agaaactggt      840

tactttttta tatatggtca ggttttatat actgataaga cctacgccat gggacatcta      900

attcagagga agaaggtcca tgtctttggg gatgaattga gtctggtgac tttgtttcga      960

tgtattcaaa atatgcctga aacactaccc aataattcct gctattcagc tggcattgca     1020

aaactggaag aaggagatga actccaactt gcaataccaa gagaaaatgc acaaatatca     1080

ctggatggag atgtcacatt ttttggtgca ttgaaactgc tgtgacctac ttacaccatg     1140

tctgtagcta ttttcctccc tttctctgta cctctaagaa gaaagaatct aactgaaaat     1200

accaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagtagtt accattgcct tttctgtgag     1260

ctatttgttt tggtttgctg aaactagtcc aaaacaggaa atttaacaga cagccacagc     1320

caaagagtgt catgtgaatt acaagaaata gagcccattt agggaaagat agaactagaa     1380

aggcttttca ttataattcc atgttgaaca attgagtcat agcttcttat cttggaggaa     1440

ggacacaatt caaaggggca gtaaggattt tgtaaaacgt ggcatccata atttactatg     1500

gagcaagtgc ccacatctct aggacattaa gacatttatg agaaatctca ggattcatct     1560

tctgttttta tgttaaatgc actccctcct tttcagttaa cattataaaa agtaaaaaat     1620

gaaaatttta gaaatcttgc attagacaca tgaaaaaata actaaaagtt taaatttaaa     1680

tatgaaacaa ttttgctgaa aatagtatcc atatactatt taagtctttt atggttattt     1740

caagtataca atttctatct gtaatgtaat atattaccca cacatttttt tcacaggaga     1800

gagagaatat cctcatttgt ttatgctcat gtgtattttc tatagtgaat ttcagaaact     1860

tttaatatca ggtaatttca atttatgcct ataaagcatt gattgaaaaa taactagaat     1920

tgtgcatata taacacataa tctccaacag aagttactga atacattcat actaatgtaa     1980

tgtaatttcc ctttatttct tgctcttctg tttcaaactg ctgctattgt agtttacata     2040

tcccaacctt taaaaatatt cctcttatta gctttatatt cactttatag aagttgagtt     2100

ttaattaaaa ttcttggcat cctgaagtat gtcacatagc atgtgctcct tataaatatg     2160

ttgatatctc agaagacagc atcccggttt tcattttata aagtaccata cttaagaatg     2220

ctgtaatact tatcttttat aacatgtttc cttcgctttg cttgtctttt atgtcatcag     2280

ttttaactgt ttacttcatt taacagttta catcattcaa cagtttactt cattaaacag     2340

taggtggaaa aatagatgcc agtctatgaa aatcttccca tctatatcaa aatacttttc     2400

aaggatatac ttttcaaaac aaacgattta aattttatgt ttaaaatata aactttagat     2460

ttaaacttta tttaaatatc tggttcctat gattttgact tcagtaagtt caaataaaat     2520

atattttgca attcattttt acattataat ttaaaaagaa gaagcgataa gtggagtcag     2580

tttcaatgct aggtggggtg gttaatgatt tttctggtgt tgctgctaat gtggattaac     2640

aaataaaaac attcattgcc ttttgcctca taaaa                                2675


<210>  5
<211>  285
<212>  PRT
<213>  Homo sapiens

<400>  5

Met Asp Asp Ser Thr Glu Arg Glu Gln Ser Arg Leu Thr Ser Cys Leu 
1               5                   10                  15      


Lys Lys Arg Glu Glu Met Lys Leu Lys Glu Cys Val Ser Ile Leu Pro 
            20                  25                  30          


Arg Lys Glu Ser Pro Ser Val Arg Ser Ser Lys Asp Gly Lys Leu Leu 
        35                  40                  45              


Ala Ala Thr Leu Leu Leu Ala Leu Leu Ser Cys Cys Leu Thr Val Val 
    50                  55                  60                  


Ser Phe Tyr Gln Val Ala Ala Leu Gln Gly Asp Leu Ala Ser Leu Arg 
65                  70                  75                  80  


Ala Glu Leu Gln Gly His His Ala Glu Lys Leu Pro Ala Gly Ala Gly 
                85                  90                  95      


Ala Pro Lys Ala Gly Leu Glu Glu Ala Pro Ala Val Thr Ala Gly Leu 
            100                 105                 110         


Lys Ile Phe Glu Pro Pro Ala Pro Gly Glu Gly Asn Ser Ser Gln Asn 
        115                 120                 125             


Ser Arg Asn Lys Arg Ala Val Gln Gly Pro Glu Glu Thr Val Thr Gln 
    130                 135                 140                 


Asp Cys Leu Gln Leu Ile Ala Asp Ser Glu Thr Pro Thr Ile Gln Lys 
145                 150                 155                 160 


Gly Ser Tyr Thr Phe Val Pro Trp Leu Leu Ser Phe Lys Arg Gly Ser 
                165                 170                 175     


Ala Leu Glu Glu Lys Glu Asn Lys Ile Leu Val Lys Glu Thr Gly Tyr 
            180                 185                 190         


Phe Phe Ile Tyr Gly Gln Val Leu Tyr Thr Asp Lys Thr Tyr Ala Met 
        195                 200                 205             


Gly His Leu Ile Gln Arg Lys Lys Val His Val Phe Gly Asp Glu Leu 
    210                 215                 220                 


Ser Leu Val Thr Leu Phe Arg Cys Ile Gln Asn Met Pro Glu Thr Leu 
225                 230                 235                 240 


Pro Asn Asn Ser Cys Tyr Ser Ala Gly Ile Ala Lys Leu Glu Glu Gly 
                245                 250                 255     


Asp Glu Leu Gln Leu Ala Ile Pro Arg Glu Asn Ala Gln Ile Ser Leu 
            260                 265                 270         


Asp Gly Asp Val Thr Phe Phe Gly Ala Leu Lys Leu Leu 
        275                 280                 285 


<210>  6
<211>  266
<212>  PRT
<213>  Homo sapiens

<400>  6

Met Asp Asp Ser Thr Glu Arg Glu Gln Ser Arg Leu Thr Ser Cys Leu 
1               5                   10                  15      


Lys Lys Arg Glu Glu Met Lys Leu Lys Glu Cys Val Ser Ile Leu Pro 
            20                  25                  30          


Arg Lys Glu Ser Pro Ser Val Arg Ser Ser Lys Asp Gly Lys Leu Leu 
        35                  40                  45              


Ala Ala Thr Leu Leu Leu Ala Leu Leu Ser Cys Cys Leu Thr Val Val 
    50                  55                  60                  


Ser Phe Tyr Gln Val Ala Ala Leu Gln Gly Asp Leu Ala Ser Leu Arg 
65                  70                  75                  80  


Ala Glu Leu Gln Gly His His Ala Glu Lys Leu Pro Ala Gly Ala Gly 
                85                  90                  95      


Ala Pro Lys Ala Gly Leu Glu Glu Ala Pro Ala Val Thr Ala Gly Leu 
            100                 105                 110         


Lys Ile Phe Glu Pro Pro Ala Pro Gly Glu Gly Asn Ser Ser Gln Asn 
        115                 120                 125             


Ser Arg Asn Lys Arg Ala Val Gln Gly Pro Glu Glu Thr Gly Ser Tyr 
    130                 135                 140                 


Thr Phe Val Pro Trp Leu Leu Ser Phe Lys Arg Gly Ser Ala Leu Glu 
145                 150                 155                 160 


Glu Lys Glu Asn Lys Ile Leu Val Lys Glu Thr Gly Tyr Phe Phe Ile 
                165                 170                 175     


Tyr Gly Gln Val Leu Tyr Thr Asp Lys Thr Tyr Ala Met Gly His Leu 
            180                 185                 190         


Ile Gln Arg Lys Lys Val His Val Phe Gly Asp Glu Leu Ser Leu Val 
        195                 200                 205             


Thr Leu Phe Arg Cys Ile Gln Asn Met Pro Glu Thr Leu Pro Asn Asn 
    210                 215                 220                 


Ser Cys Tyr Ser Ala Gly Ile Ala Lys Leu Glu Glu Gly Asp Glu Leu 
225                 230                 235                 240 


Gln Leu Ala Ile Pro Arg Glu Asn Ala Gln Ile Ser Leu Asp Gly Asp 
                245                 250                 255     


Val Thr Phe Phe Gly Ala Leu Lys Leu Leu 
            260                 265     


<210>  7
<211>  278
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized BAFF Protein

<400>  7

Met Asp Glu Ser Ala Lys Thr Leu Pro Pro Pro Cys Leu Cys Phe Cys 
1               5                   10                  15      


Ser Glu Lys Gly Glu Asp Met Lys Val Gly Tyr Asp Pro Ile Thr Pro 
            20                  25                  30          


Gln Lys Glu Glu Gly Ala Trp Phe Gly Ile Cys Arg Asp Gly Arg Leu 
        35                  40                  45              


Leu Ala Ala Thr Leu Leu Leu Ala Leu Leu Ser Ser Ser Phe Thr Ala 
    50                  55                  60                  


Met Ser Leu Tyr Gln Leu Ala Ala Leu Gln Ala Asp Leu Met Asn Leu 
65                  70                  75                  80  


Arg Met Glu Leu Gln Ser Tyr Arg Gly Ser Ala Thr Pro Ala Ala Ala 
                85                  90                  95      


Gly Ala Pro Glu Leu Thr Ala Gly Val Lys Leu Leu Thr Pro Ala Ala 
            100                 105                 110         


Pro Arg Pro His Asn Ser Ser Arg Gly His Arg Asn Arg Arg Ala Phe 
        115                 120                 125             


Gln Gly Pro Glu Glu Thr Val Thr Gln Asp Cys Leu Gln Leu Ile Ala 
    130                 135                 140                 


Asp Ser Glu Thr Pro Thr Ile Gln Lys Gly Ser Tyr Thr Phe Val Pro 
145                 150                 155                 160 


Trp Leu Leu Ser Phe Lys Arg Gly Ser Ala Leu Glu Glu Lys Glu Asn 
                165                 170                 175     


Lys Ile Leu Val Lys Glu Thr Gly Tyr Phe Phe Ile Tyr Gly Gln Val 
            180                 185                 190         


Leu Tyr Thr Asp Lys Thr Tyr Ala Met Gly His Leu Ile Gln Arg Lys 
        195                 200                 205             


Lys Val His Val Phe Gly Asp Glu Leu Ser Leu Val Thr Leu Phe Arg 
    210                 215                 220                 


Cys Ile Gln Asn Met Pro Glu Thr Leu Pro Asn Asn Ser Cys Tyr Ser 
225                 230                 235                 240 


Ala Gly Ile Ala Lys Leu Glu Glu Gly Asp Glu Leu Gln Leu Ala Ile 
                245                 250                 255     


Pro Arg Glu Asn Ala Gln Ile Ser Leu Asp Gly Asp Val Thr Phe Phe 
            260                 265                 270         


Gly Ala Leu Lys Leu Leu 
        275             


<210>  8
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide: mBaff-1, mBaff intron 2, Forward

<400>  8
ggacagcaga taggaaagct tcttg                                             25


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide: mBaff-1, mBaff intron 2, Reverse

<400>  9
gggacggaca ctcatttgac                                                   20


<210>  10
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide: mBaff-1, mBaff intron 2, Probe

<400>  10
taggaatccc agtccttaga accgca                                            26


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide: mBaff-2, mBaff exon 7, Forward

<400>  11
cctcgggaga atgcacagat                                                   20


<210>  12
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide: mBaff-2, mBaff exon 7, Reverse

<400>  12
gcactccagc aagtgagtta c                                                 21


<210>  13
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide: mBaff-2, mBaff exon 7, Probe

<400>  13
tcacgcaacg gagacgacac ctt                                               23


<210>  14
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide: hBaff-1, hBAFF intron 2, Forward

<400>  14
ccggttggca tttctggctt ag                                                22


<210>  15
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide: hBaff-1, hBAFF intron 2, Reverse

<400>  15
ggctggatgg tcaagttcta ca                                                22


<210>  16
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide: hBaff-1, hBAFF intron 2, Probe

<400>  16
ttccaggctg taacatgagt gttgga                                            26


<210>  17
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide: hBaff-2, hBAFF intron 5, Forward

<400>  17
acaccagaca ggtgacttag gaa                                               23


<210>  18
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide: hBaff-2, hBAFF intron 5, Reverse

<400>  18
gctcctgggt gcaaaggta                                                    19


<210>  19
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide: hBaff-2, hBAFF intron 5, Probe

<400>  19
tgcgaaagtg taggcgcaaa cc                                                22


