                         SEQUENCE LISTING

<110>  Sandoz AG
 
<120>  Chemoenzymatic synthesis of the cephalosporine intermediate 
       7-amino-3-alkenylcephem-4-carboxylic acid

<130>  30A-127 363

<160>  1     

<170>  PatentIn version 3.5

<210>  1
<211>  489
<212>  PRT
<213>  Bacillus subtilis

<400>  1

Met Thr His Gln Ile Val Thr Thr Gln Tyr Gly Lys Val Lys Gly Thr 
1               5                   10                  15      


Thr Glu Asn Gly Val His Lys Trp Lys Gly Ile Pro Tyr Ala Lys Pro 
            20                  25                  30          


Pro Val Gly Gln Trp Arg Phe Lys Ala Pro Glu Pro Pro Glu Val Trp 
        35                  40                  45              


Glu Asp Val Leu Asp Ala Thr Ala Tyr Gly Pro Ile Cys Pro Gln Pro 
    50                  55                  60                  


Ser Asp Leu Leu Ser Leu Ser Tyr Thr Glu Leu Pro Arg Gln Ser Glu 
65                  70                  75                  80  


Asp Cys Leu Tyr Val Asn Val Phe Ala Pro Asp Thr Pro Ser Gln Asn 
                85                  90                  95      


Leu Pro Val Met Val Trp Ile His Gly Gly Ala Phe Tyr Leu Gly Ala 
            100                 105                 110         


Gly Ser Glu Pro Leu Tyr Asp Gly Ser Lys Leu Ala Ala Gln Gly Glu 
        115                 120                 125             


Val Ile Val Val Thr Leu Asn Tyr Arg Leu Gly Pro Phe Gly Phe Leu 
    130                 135                 140                 


His Leu Ser Ser Phe Asp Glu Ala Tyr Ser Asp Asn Leu Gly Leu Leu 
145                 150                 155                 160 


Asp Gln Ala Ala Ala Leu Lys Trp Val Arg Glu Asn Ile Ser Ala Phe 
                165                 170                 175     


Gly Gly Asp Pro Asp Asn Val Thr Val Phe Gly Glu Ser Ala Gly Gly 
            180                 185                 190         


Met Ser Ile Ala Ala Leu Leu Ala Met Pro Ala Ala Lys Gly Leu Phe 
        195                 200                 205             


Gln Lys Ala Ile Met Glu Ser Gly Ala Ser Arg Thr Met Thr Lys Glu 
    210                 215                 220                 


Gln Ala Ala Ser Thr Ala Ala Ala Phe Leu Gln Val Leu Gly Ile Asn 
225                 230                 235                 240 


Glu Ser Gln Leu Asp Arg Leu His Thr Val Ala Ala Glu Asp Leu Leu 
                245                 250                 255     


Lys Ala Ala Asp Gln Leu Arg Ile Ala Glu Lys Glu Asn Ile Phe Gln 
            260                 265                 270         


Leu Phe Phe Gln Pro Ala Leu Asp Pro Lys Thr Leu Pro Glu Glu Pro 
        275                 280                 285             


Glu Lys Ser Ile Ala Glu Gly Ala Ala Ser Gly Ile Pro Leu Leu Ile 
    290                 295                 300                 


Gly Thr Thr Arg Asp Glu Gly Tyr Leu Phe Phe Thr Pro Asp Ser Asp 
305                 310                 315                 320 


Val His Ser Gln Glu Thr Leu Asp Ala Ala Leu Glu Tyr Leu Leu Gly 
                325                 330                 335     


Lys Pro Leu Ala Glu Lys Ala Ala Asp Leu Tyr Pro Arg Ser Leu Glu 
            340                 345                 350         


Ser Gln Ile His Met Met Thr Asp Leu Leu Phe Trp Arg Pro Ala Val 
        355                 360                 365             


Ala Tyr Ala Ser Ala Gln Ser His Tyr Ala Pro Val Trp Met Tyr Arg 
    370                 375                 380                 


Phe Asp Trp His Pro Glu Lys Pro Pro Tyr Asn Lys Ala Phe His Ala 
385                 390                 395                 400 


Leu Glu Leu Pro Phe Val Phe Gly Asn Leu Asp Gly Leu Glu Arg Met 
                405                 410                 415     


Ala Lys Ala Glu Ile Thr Asp Glu Val Lys Gln Leu Ser His Thr Ile 
            420                 425                 430         


Gln Ser Ala Trp Ile Thr Phe Ala Lys Thr Gly Asn Pro Ser Thr Glu 
        435                 440                 445             


Ala Val Asn Trp Pro Ala Tyr His Glu Glu Thr Arg Glu Thr Val Ile 
    450                 455                 460                 


Leu Asp Ser Glu Ile Thr Ile Glu Asn Asp Pro Glu Ser Glu Lys Arg 
465                 470                 475                 480 


Gln Lys Leu Phe Pro Ser Lys Gly Glu 
                485                 


