                         SEQUENCE LISTING

<110>  Lysando AG
 
<120>  Modified EL188 endolysin sequence

<130>  LYS-022 PCT

<160>  182   

<170>  PatentIn version 3.5

<210>  1
<211>  292
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus Sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (120)..(120)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (126)..(126)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (128)..(128)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (134)..(135)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (138)..(138)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (171)..(171)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (173)..(173)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (182)..(182)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (187)..(187)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (197)..(198)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (204)..(204)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (230)..(231)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (234)..(234)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (241)..(241)
<223>  Xaa can be any naturally occurring amino acid

<400>  1

Xaa Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val 
1               5                   10                  15      


Lys Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys 
            20                  25                  30          


Gly Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val 
        35                  40                  45              


Val Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala 
    50                  55                  60                  


Ser Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu 
65                  70                  75                  80  


Gly Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu 
                85                  90                  95      


Ser Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr 
            100                 105                 110         


Pro Thr Tyr Asp Ile Ala Trp Xaa Gly Lys Val Ser Pro Xaa Phe Xaa 
        115                 120                 125             


Ala Lys Val Lys Asp Xaa Xaa Gly Val Xaa Val Pro Asn His Arg Ala 
    130                 135                 140                 


Pro His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe 
145                 150                 155                 160 


Ser Pro Ser Ile Lys Asn Ala Ala Gly Ser Xaa Ala Xaa Gly Leu Ile 
                165                 170                 175     


Gln Phe Met Ser Pro Xaa Ala Asn Asp Leu Xaa Val Pro Leu Ser Val 
            180                 185                 190         


Ile Arg Ser Met Xaa Xaa Leu Thr Gln Leu Asp Xaa Val Phe Lys Tyr 
        195                 200                 205             


Phe Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp 
    210                 215                 220                 


Phe Tyr Leu Thr Ile Xaa Xaa Pro Ala Xaa Val Gly Lys Lys Ala Asp 
225                 230                 235                 240 


Xaa Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly 
                245                 250                 255     


Phe Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser 
            260                 265                 270         


Thr Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His 
        275                 280                 285             


Val Ile Ser Tyr 
    290         


<210>  2
<211>  291
<212>  PRT
<213>  Artificial sequence

<220>
<223>  EL188 endolysin without N-terminal methionine

<400>  2

Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys 
1               5                   10                  15      


Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly 
            20                  25                  30          


Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val Val 
        35                  40                  45              


Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser 
    50                  55                  60                  


Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly 
65                  70                  75                  80  


Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser 
                85                  90                  95      


Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro 
            100                 105                 110         


Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr Ala 
        115                 120                 125             


Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala Pro 
    130                 135                 140                 


His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe Ser 
145                 150                 155                 160 


Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln 
                165                 170                 175     


Phe Met Ser Pro Ala Ala Asn Asp Leu Asn Val Pro Leu Ser Val Ile 
            180                 185                 190         


Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe 
        195                 200                 205             


Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe 
    210                 215                 220                 


Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp Glu 
225                 230                 235                 240 


Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe 
                245                 250                 255     


Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr 
            260                 265                 270         


Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His Val 
        275                 280                 285             


Ile Ser Tyr 
    290     


<210>  3
<211>  292
<212>  PRT
<213>  Unknown

<220>
<223>  ELgp188

<400>  3

Met Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val 
1               5                   10                  15      


Lys Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys 
            20                  25                  30          


Gly Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val 
        35                  40                  45              


Val Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala 
    50                  55                  60                  


Ser Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu 
65                  70                  75                  80  


Gly Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu 
                85                  90                  95      


Ser Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr 
            100                 105                 110         


Pro Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr 
        115                 120                 125             


Ala Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala 
    130                 135                 140                 


Pro His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe 
145                 150                 155                 160 


Ser Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile 
                165                 170                 175     


Gln Phe Met Ser Pro Ala Ala Asn Asp Leu Asn Val Pro Leu Ser Val 
            180                 185                 190         


Ile Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr 
        195                 200                 205             


Phe Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp 
    210                 215                 220                 


Phe Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp 
225                 230                 235                 240 


Glu Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly 
                245                 250                 255     


Phe Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser 
            260                 265                 270         


Thr Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His 
        275                 280                 285             


Val Ile Ser Tyr 
    290         


<210>  4
<211>  292
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with S120G

<400>  4

Met Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val 
1               5                   10                  15      


Lys Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys 
            20                  25                  30          


Gly Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val 
        35                  40                  45              


Val Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala 
    50                  55                  60                  


Ser Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu 
65                  70                  75                  80  


Gly Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu 
                85                  90                  95      


Ser Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr 
            100                 105                 110         


Pro Thr Tyr Asp Ile Ala Trp Gly Gly Lys Val Ser Pro Ala Phe Thr 
        115                 120                 125             


Ala Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala 
    130                 135                 140                 


Pro His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe 
145                 150                 155                 160 


Ser Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile 
                165                 170                 175     


Gln Phe Met Ser Pro Ala Ala Asn Asp Leu Asn Val Pro Leu Ser Val 
            180                 185                 190         


Ile Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr 
        195                 200                 205             


Phe Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp 
    210                 215                 220                 


Phe Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp 
225                 230                 235                 240 


Glu Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly 
                245                 250                 255     


Phe Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser 
            260                 265                 270         


Thr Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His 
        275                 280                 285             


Val Ile Ser Tyr 
    290         


<210>  5
<211>  294
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with A126E and T128K

<400>  5

Met Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val 
1               5                   10                  15      


Lys Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys 
            20                  25                  30          


Gly Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val 
        35                  40                  45              


Val Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala 
    50                  55                  60                  


Ser Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu 
65                  70                  75                  80  


Gly Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu 
                85                  90                  95      


Ser Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr 
            100                 105                 110         


Pro Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Glu Phe Lys 
        115                 120                 125             


Ala Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala 
    130                 135                 140                 


Pro His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe 
145                 150                 155                 160 


Ser Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile 
                165                 170                 175     


Gln Phe Met Ser Pro Ala Ala Asn Asp Leu Asn Val Pro Leu Ser Val 
            180                 185                 190         


Ile Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr 
        195                 200                 205             


Phe Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp 
    210                 215                 220                 


Phe Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp 
225                 230                 235                 240 


Glu Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly 
                245                 250                 255     


Phe Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser 
            260                 265                 270         


Thr Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His 
        275                 280                 285             


Val Ile Ser Tyr Leu Glu 
    290                 


<210>  6
<211>  294
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with W134I

<400>  6

Met Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val 
1               5                   10                  15      


Lys Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys 
            20                  25                  30          


Gly Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val 
        35                  40                  45              


Val Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala 
    50                  55                  60                  


Ser Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu 
65                  70                  75                  80  


Gly Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu 
                85                  90                  95      


Ser Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr 
            100                 105                 110         


Pro Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr 
        115                 120                 125             


Ala Lys Val Lys Asp Ile Cys Gly Val His Val Pro Asn His Arg Ala 
    130                 135                 140                 


Pro His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe 
145                 150                 155                 160 


Ser Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile 
                165                 170                 175     


Gln Phe Met Ser Pro Ala Ala Asn Asp Leu Asn Val Pro Leu Ser Val 
            180                 185                 190         


Ile Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr 
        195                 200                 205             


Phe Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp 
    210                 215                 220                 


Phe Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp 
225                 230                 235                 240 


Glu Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly 
                245                 250                 255     


Phe Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser 
            260                 265                 270         


Thr Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His 
        275                 280                 285             


Val Ile Ser Tyr Leu Glu 
    290                 


<210>  7
<211>  294
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with H138L

<400>  7

Met Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val 
1               5                   10                  15      


Lys Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys 
            20                  25                  30          


Gly Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val 
        35                  40                  45              


Val Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala 
    50                  55                  60                  


Ser Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu 
65                  70                  75                  80  


Gly Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu 
                85                  90                  95      


Ser Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr 
            100                 105                 110         


Pro Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr 
        115                 120                 125             


Ala Lys Val Lys Asp Trp Cys Gly Val Leu Val Pro Asn His Arg Ala 
    130                 135                 140                 


Pro His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe 
145                 150                 155                 160 


Ser Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile 
                165                 170                 175     


Gln Phe Met Ser Pro Ala Ala Asn Asp Leu Asn Val Pro Leu Ser Val 
            180                 185                 190         


Ile Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr 
        195                 200                 205             


Phe Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp 
    210                 215                 220                 


Phe Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp 
225                 230                 235                 240 


Glu Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly 
                245                 250                 255     


Phe Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser 
            260                 265                 270         


Thr Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His 
        275                 280                 285             


Val Ile Ser Tyr Leu Glu 
    290                 


<210>  8
<211>  294
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with E171G

<400>  8

Met Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val 
1               5                   10                  15      


Lys Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys 
            20                  25                  30          


Gly Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val 
        35                  40                  45              


Val Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala 
    50                  55                  60                  


Ser Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu 
65                  70                  75                  80  


Gly Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu 
                85                  90                  95      


Ser Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr 
            100                 105                 110         


Pro Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr 
        115                 120                 125             


Ala Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala 
    130                 135                 140                 


Pro His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe 
145                 150                 155                 160 


Ser Pro Ser Ile Lys Asn Ala Ala Gly Ser Gly Ala Tyr Gly Leu Ile 
                165                 170                 175     


Gln Phe Met Ser Pro Ala Ala Asn Asp Leu Asn Val Pro Leu Ser Val 
            180                 185                 190         


Ile Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr 
        195                 200                 205             


Phe Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp 
    210                 215                 220                 


Phe Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp 
225                 230                 235                 240 


Glu Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly 
                245                 250                 255     


Phe Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser 
            260                 265                 270         


Thr Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His 
        275                 280                 285             


Val Ile Ser Tyr Leu Glu 
    290                 


<210>  9
<211>  294
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with Y173T

<400>  9

Met Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val 
1               5                   10                  15      


Lys Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys 
            20                  25                  30          


Gly Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val 
        35                  40                  45              


Val Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala 
    50                  55                  60                  


Ser Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu 
65                  70                  75                  80  


Gly Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu 
                85                  90                  95      


Ser Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr 
            100                 105                 110         


Pro Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr 
        115                 120                 125             


Ala Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala 
    130                 135                 140                 


Pro His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe 
145                 150                 155                 160 


Ser Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Thr Gly Leu Ile 
                165                 170                 175     


Gln Phe Met Ser Pro Ala Ala Asn Asp Leu Asn Val Pro Leu Ser Val 
            180                 185                 190         


Ile Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr 
        195                 200                 205             


Phe Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp 
    210                 215                 220                 


Phe Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp 
225                 230                 235                 240 


Glu Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly 
                245                 250                 255     


Phe Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser 
            260                 265                 270         


Thr Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His 
        275                 280                 285             


Val Ile Ser Tyr Leu Glu 
    290                 


<210>  10
<211>  294
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with A182T

<400>  10

Met Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val 
1               5                   10                  15      


Lys Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys 
            20                  25                  30          


Gly Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val 
        35                  40                  45              


Val Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala 
    50                  55                  60                  


Ser Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu 
65                  70                  75                  80  


Gly Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu 
                85                  90                  95      


Ser Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr 
            100                 105                 110         


Pro Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr 
        115                 120                 125             


Ala Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala 
    130                 135                 140                 


Pro His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe 
145                 150                 155                 160 


Ser Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile 
                165                 170                 175     


Gln Phe Met Ser Pro Thr Ala Asn Asp Leu Asn Val Pro Leu Ser Val 
            180                 185                 190         


Ile Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr 
        195                 200                 205             


Phe Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp 
    210                 215                 220                 


Phe Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp 
225                 230                 235                 240 


Glu Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly 
                245                 250                 255     


Phe Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser 
            260                 265                 270         


Thr Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His 
        275                 280                 285             


Val Ile Ser Tyr Leu Glu 
    290                 


<210>  11
<211>  294
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with N187G

<400>  11

Met Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val 
1               5                   10                  15      


Lys Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys 
            20                  25                  30          


Gly Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val 
        35                  40                  45              


Val Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala 
    50                  55                  60                  


Ser Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu 
65                  70                  75                  80  


Gly Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu 
                85                  90                  95      


Ser Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr 
            100                 105                 110         


Pro Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr 
        115                 120                 125             


Ala Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala 
    130                 135                 140                 


Pro His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe 
145                 150                 155                 160 


Ser Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile 
                165                 170                 175     


Gln Phe Met Ser Pro Ala Ala Asn Asp Leu Gly Val Pro Leu Ser Val 
            180                 185                 190         


Ile Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr 
        195                 200                 205             


Phe Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp 
    210                 215                 220                 


Phe Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp 
225                 230                 235                 240 


Glu Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly 
                245                 250                 255     


Phe Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser 
            260                 265                 270         


Thr Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His 
        275                 280                 285             


Val Ile Ser Tyr Leu Glu 
    290                 


<210>  12
<211>  294
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with D197S and Q198A

<400>  12

Met Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val 
1               5                   10                  15      


Lys Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys 
            20                  25                  30          


Gly Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val 
        35                  40                  45              


Val Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala 
    50                  55                  60                  


Ser Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu 
65                  70                  75                  80  


Gly Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu 
                85                  90                  95      


Ser Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr 
            100                 105                 110         


Pro Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr 
        115                 120                 125             


Ala Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala 
    130                 135                 140                 


Pro His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe 
145                 150                 155                 160 


Ser Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile 
                165                 170                 175     


Gln Phe Met Ser Pro Ala Ala Asn Asp Leu Asn Val Pro Leu Ser Val 
            180                 185                 190         


Ile Arg Ser Met Ser Ala Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr 
        195                 200                 205             


Phe Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp 
    210                 215                 220                 


Phe Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp 
225                 230                 235                 240 


Glu Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly 
                245                 250                 255     


Phe Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser 
            260                 265                 270         


Thr Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His 
        275                 280                 285             


Val Ile Ser Tyr Leu Glu 
    290                 


<210>  13
<211>  294
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with L204Y

<400>  13

Met Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val 
1               5                   10                  15      


Lys Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys 
            20                  25                  30          


Gly Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val 
        35                  40                  45              


Val Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala 
    50                  55                  60                  


Ser Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu 
65                  70                  75                  80  


Gly Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu 
                85                  90                  95      


Ser Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr 
            100                 105                 110         


Pro Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr 
        115                 120                 125             


Ala Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala 
    130                 135                 140                 


Pro His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe 
145                 150                 155                 160 


Ser Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile 
                165                 170                 175     


Gln Phe Met Ser Pro Ala Ala Asn Asp Leu Asn Val Pro Leu Ser Val 
            180                 185                 190         


Ile Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Tyr Val Phe Lys Tyr 
        195                 200                 205             


Phe Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp 
    210                 215                 220                 


Phe Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp 
225                 230                 235                 240 


Glu Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly 
                245                 250                 255     


Phe Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser 
            260                 265                 270         


Thr Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His 
        275                 280                 285             


Val Ile Ser Tyr Leu Glu 
    290                 


<210>  14
<211>  294
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with F230L

<400>  14

Met Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val 
1               5                   10                  15      


Lys Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys 
            20                  25                  30          


Gly Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val 
        35                  40                  45              


Val Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala 
    50                  55                  60                  


Ser Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu 
65                  70                  75                  80  


Gly Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu 
                85                  90                  95      


Ser Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr 
            100                 105                 110         


Pro Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr 
        115                 120                 125             


Ala Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala 
    130                 135                 140                 


Pro His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe 
145                 150                 155                 160 


Ser Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile 
                165                 170                 175     


Gln Phe Met Ser Pro Ala Ala Asn Asp Leu Asn Val Pro Leu Ser Val 
            180                 185                 190         


Ile Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr 
        195                 200                 205             


Phe Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp 
    210                 215                 220                 


Phe Tyr Leu Thr Ile Leu His Pro Ala Ser Val Gly Lys Lys Ala Asp 
225                 230                 235                 240 


Glu Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly 
                245                 250                 255     


Phe Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser 
            260                 265                 270         


Thr Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His 
        275                 280                 285             


Val Ile Ser Tyr Leu Glu 
    290                 


<210>  15
<211>  294
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with H231Y

<400>  15

Met Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val 
1               5                   10                  15      


Lys Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys 
            20                  25                  30          


Gly Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val 
        35                  40                  45              


Val Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala 
    50                  55                  60                  


Ser Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu 
65                  70                  75                  80  


Gly Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu 
                85                  90                  95      


Ser Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr 
            100                 105                 110         


Pro Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr 
        115                 120                 125             


Ala Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala 
    130                 135                 140                 


Pro His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe 
145                 150                 155                 160 


Ser Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile 
                165                 170                 175     


Gln Phe Met Ser Pro Ala Ala Asn Asp Leu Asn Val Pro Leu Ser Val 
            180                 185                 190         


Ile Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr 
        195                 200                 205             


Phe Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp 
    210                 215                 220                 


Phe Tyr Leu Thr Ile Phe Tyr Pro Ala Ser Val Gly Lys Lys Ala Asp 
225                 230                 235                 240 


Glu Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly 
                245                 250                 255     


Phe Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser 
            260                 265                 270         


Thr Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His 
        275                 280                 285             


Val Ile Ser Tyr Leu Glu 
    290                 


<210>  16
<211>  294
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with S234A

<400>  16

Met Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val 
1               5                   10                  15      


Lys Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys 
            20                  25                  30          


Gly Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val 
        35                  40                  45              


Val Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala 
    50                  55                  60                  


Ser Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu 
65                  70                  75                  80  


Gly Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu 
                85                  90                  95      


Ser Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr 
            100                 105                 110         


Pro Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr 
        115                 120                 125             


Ala Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala 
    130                 135                 140                 


Pro His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe 
145                 150                 155                 160 


Ser Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile 
                165                 170                 175     


Gln Phe Met Ser Pro Ala Ala Asn Asp Leu Asn Val Pro Leu Ser Val 
            180                 185                 190         


Ile Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr 
        195                 200                 205             


Phe Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp 
    210                 215                 220                 


Phe Tyr Leu Thr Ile Phe His Pro Ala Ala Val Gly Lys Lys Ala Asp 
225                 230                 235                 240 


Glu Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly 
                245                 250                 255     


Phe Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser 
            260                 265                 270         


Thr Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His 
        275                 280                 285             


Val Ile Ser Tyr Leu Glu 
    290                 


<210>  17
<211>  294
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with C135S

<400>  17

Met Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val 
1               5                   10                  15      


Lys Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys 
            20                  25                  30          


Gly Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val 
        35                  40                  45              


Val Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala 
    50                  55                  60                  


Ser Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu 
65                  70                  75                  80  


Gly Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu 
                85                  90                  95      


Ser Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr 
            100                 105                 110         


Pro Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr 
        115                 120                 125             


Ala Lys Val Lys Asp Trp Ser Gly Val His Val Pro Asn His Arg Ala 
    130                 135                 140                 


Pro His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe 
145                 150                 155                 160 


Ser Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile 
                165                 170                 175     


Gln Phe Met Ser Pro Ala Ala Asn Asp Leu Asn Val Pro Leu Ser Val 
            180                 185                 190         


Ile Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr 
        195                 200                 205             


Phe Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp 
    210                 215                 220                 


Phe Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp 
225                 230                 235                 240 


Glu Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly 
                245                 250                 255     


Phe Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser 
            260                 265                 270         


Thr Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His 
        275                 280                 285             


Val Ile Ser Tyr Leu Glu 
    290                 


<210>  18
<211>  294
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with N187G and F230L

<400>  18

Met Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val 
1               5                   10                  15      


Lys Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys 
            20                  25                  30          


Gly Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val 
        35                  40                  45              


Val Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala 
    50                  55                  60                  


Ser Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu 
65                  70                  75                  80  


Gly Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu 
                85                  90                  95      


Ser Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr 
            100                 105                 110         


Pro Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr 
        115                 120                 125             


Ala Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala 
    130                 135                 140                 


Pro His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe 
145                 150                 155                 160 


Ser Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile 
                165                 170                 175     


Gln Phe Met Ser Pro Ala Ala Asn Asp Leu Gly Val Pro Leu Ser Val 
            180                 185                 190         


Ile Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr 
        195                 200                 205             


Phe Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp 
    210                 215                 220                 


Phe Tyr Leu Thr Ile Leu His Pro Ala Ser Val Gly Lys Lys Ala Asp 
225                 230                 235                 240 


Glu Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly 
                245                 250                 255     


Phe Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser 
            260                 265                 270         


Thr Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His 
        275                 280                 285             


Val Ile Ser Tyr Leu Glu 
    290                 


<210>  19
<211>  294
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with A182T, N187G and F230L

<400>  19

Met Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val 
1               5                   10                  15      


Lys Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys 
            20                  25                  30          


Gly Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val 
        35                  40                  45              


Val Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala 
    50                  55                  60                  


Ser Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu 
65                  70                  75                  80  


Gly Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu 
                85                  90                  95      


Ser Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr 
            100                 105                 110         


Pro Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr 
        115                 120                 125             


Ala Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala 
    130                 135                 140                 


Pro His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe 
145                 150                 155                 160 


Ser Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile 
                165                 170                 175     


Gln Phe Met Ser Pro Thr Ala Asn Asp Leu Gly Val Pro Leu Ser Val 
            180                 185                 190         


Ile Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr 
        195                 200                 205             


Phe Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp 
    210                 215                 220                 


Phe Tyr Leu Thr Ile Leu His Pro Ala Ser Val Gly Lys Lys Ala Asp 
225                 230                 235                 240 


Glu Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly 
                245                 250                 255     


Phe Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser 
            260                 265                 270         


Thr Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His 
        275                 280                 285             


Val Ile Ser Tyr Leu Glu 
    290                 


<210>  20
<211>  291
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with S120G and without N-terminal methionine

<400>  20

Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys 
1               5                   10                  15      


Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly 
            20                  25                  30          


Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val Val 
        35                  40                  45              


Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser 
    50                  55                  60                  


Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly 
65                  70                  75                  80  


Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser 
                85                  90                  95      


Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro 
            100                 105                 110         


Thr Tyr Asp Ile Ala Trp Gly Gly Lys Val Ser Pro Ala Phe Thr Ala 
        115                 120                 125             


Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala Pro 
    130                 135                 140                 


His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe Ser 
145                 150                 155                 160 


Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln 
                165                 170                 175     


Phe Met Ser Pro Ala Ala Asn Asp Leu Asn Val Pro Leu Ser Val Ile 
            180                 185                 190         


Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe 
        195                 200                 205             


Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe 
    210                 215                 220                 


Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp Glu 
225                 230                 235                 240 


Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe 
                245                 250                 255     


Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr 
            260                 265                 270         


Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His Val 
        275                 280                 285             


Ile Ser Tyr 
    290     


<210>  21
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with A126E and T128K and without N-terminal 
       methionine

<400>  21

Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys 
1               5                   10                  15      


Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly 
            20                  25                  30          


Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val Val 
        35                  40                  45              


Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser 
    50                  55                  60                  


Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly 
65                  70                  75                  80  


Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser 
                85                  90                  95      


Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro 
            100                 105                 110         


Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Glu Phe Lys Ala 
        115                 120                 125             


Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala Pro 
    130                 135                 140                 


His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe Ser 
145                 150                 155                 160 


Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln 
                165                 170                 175     


Phe Met Ser Pro Ala Ala Asn Asp Leu Asn Val Pro Leu Ser Val Ile 
            180                 185                 190         


Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe 
        195                 200                 205             


Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe 
    210                 215                 220                 


Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp Glu 
225                 230                 235                 240 


Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe 
                245                 250                 255     


Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr 
            260                 265                 270         


Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His Val 
        275                 280                 285             


Ile Ser Tyr Leu Glu 
    290             


<210>  22
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with W134I and without N-terminal methionine

<400>  22

Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys 
1               5                   10                  15      


Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly 
            20                  25                  30          


Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val Val 
        35                  40                  45              


Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser 
    50                  55                  60                  


Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly 
65                  70                  75                  80  


Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser 
                85                  90                  95      


Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro 
            100                 105                 110         


Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr Ala 
        115                 120                 125             


Lys Val Lys Asp Ile Cys Gly Val His Val Pro Asn His Arg Ala Pro 
    130                 135                 140                 


His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe Ser 
145                 150                 155                 160 


Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln 
                165                 170                 175     


Phe Met Ser Pro Ala Ala Asn Asp Leu Asn Val Pro Leu Ser Val Ile 
            180                 185                 190         


Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe 
        195                 200                 205             


Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe 
    210                 215                 220                 


Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp Glu 
225                 230                 235                 240 


Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe 
                245                 250                 255     


Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr 
            260                 265                 270         


Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His Val 
        275                 280                 285             


Ile Ser Tyr Leu Glu 
    290             


<210>  23
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with H138L and without N-terminal methionine

<400>  23

Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys 
1               5                   10                  15      


Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly 
            20                  25                  30          


Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val Val 
        35                  40                  45              


Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser 
    50                  55                  60                  


Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly 
65                  70                  75                  80  


Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser 
                85                  90                  95      


Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro 
            100                 105                 110         


Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr Ala 
        115                 120                 125             


Lys Val Lys Asp Trp Cys Gly Val Leu Val Pro Asn His Arg Ala Pro 
    130                 135                 140                 


His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe Ser 
145                 150                 155                 160 


Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln 
                165                 170                 175     


Phe Met Ser Pro Ala Ala Asn Asp Leu Asn Val Pro Leu Ser Val Ile 
            180                 185                 190         


Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe 
        195                 200                 205             


Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe 
    210                 215                 220                 


Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp Glu 
225                 230                 235                 240 


Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe 
                245                 250                 255     


Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr 
            260                 265                 270         


Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His Val 
        275                 280                 285             


Ile Ser Tyr Leu Glu 
    290             


<210>  24
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with E171G and without N-terminal methionine

<400>  24

Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys 
1               5                   10                  15      


Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly 
            20                  25                  30          


Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val Val 
        35                  40                  45              


Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser 
    50                  55                  60                  


Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly 
65                  70                  75                  80  


Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser 
                85                  90                  95      


Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro 
            100                 105                 110         


Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr Ala 
        115                 120                 125             


Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala Pro 
    130                 135                 140                 


His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe Ser 
145                 150                 155                 160 


Pro Ser Ile Lys Asn Ala Ala Gly Ser Gly Ala Tyr Gly Leu Ile Gln 
                165                 170                 175     


Phe Met Ser Pro Ala Ala Asn Asp Leu Asn Val Pro Leu Ser Val Ile 
            180                 185                 190         


Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe 
        195                 200                 205             


Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe 
    210                 215                 220                 


Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp Glu 
225                 230                 235                 240 


Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe 
                245                 250                 255     


Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr 
            260                 265                 270         


Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His Val 
        275                 280                 285             


Ile Ser Tyr Leu Glu 
    290             


<210>  25
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with Y173T and without N-terminal methionine

<400>  25

Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys 
1               5                   10                  15      


Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly 
            20                  25                  30          


Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val Val 
        35                  40                  45              


Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser 
    50                  55                  60                  


Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly 
65                  70                  75                  80  


Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser 
                85                  90                  95      


Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro 
            100                 105                 110         


Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr Ala 
        115                 120                 125             


Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala Pro 
    130                 135                 140                 


His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe Ser 
145                 150                 155                 160 


Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Thr Gly Leu Ile Gln 
                165                 170                 175     


Phe Met Ser Pro Ala Ala Asn Asp Leu Asn Val Pro Leu Ser Val Ile 
            180                 185                 190         


Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe 
        195                 200                 205             


Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe 
    210                 215                 220                 


Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp Glu 
225                 230                 235                 240 


Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe 
                245                 250                 255     


Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr 
            260                 265                 270         


Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His Val 
        275                 280                 285             


Ile Ser Tyr Leu Glu 
    290             


<210>  26
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with A182T and without N-terminal methionine

<400>  26

Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys 
1               5                   10                  15      


Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly 
            20                  25                  30          


Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val Val 
        35                  40                  45              


Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser 
    50                  55                  60                  


Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly 
65                  70                  75                  80  


Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser 
                85                  90                  95      


Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro 
            100                 105                 110         


Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr Ala 
        115                 120                 125             


Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala Pro 
    130                 135                 140                 


His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe Ser 
145                 150                 155                 160 


Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln 
                165                 170                 175     


Phe Met Ser Pro Thr Ala Asn Asp Leu Asn Val Pro Leu Ser Val Ile 
            180                 185                 190         


Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe 
        195                 200                 205             


Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe 
    210                 215                 220                 


Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp Glu 
225                 230                 235                 240 


Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe 
                245                 250                 255     


Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr 
            260                 265                 270         


Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His Val 
        275                 280                 285             


Ile Ser Tyr Leu Glu 
    290             


<210>  27
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with N187G and without N-terminal methionine

<400>  27

Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys 
1               5                   10                  15      


Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly 
            20                  25                  30          


Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val Val 
        35                  40                  45              


Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser 
    50                  55                  60                  


Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly 
65                  70                  75                  80  


Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser 
                85                  90                  95      


Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro 
            100                 105                 110         


Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr Ala 
        115                 120                 125             


Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala Pro 
    130                 135                 140                 


His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe Ser 
145                 150                 155                 160 


Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln 
                165                 170                 175     


Phe Met Ser Pro Ala Ala Asn Asp Leu Gly Val Pro Leu Ser Val Ile 
            180                 185                 190         


Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe 
        195                 200                 205             


Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe 
    210                 215                 220                 


Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp Glu 
225                 230                 235                 240 


Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe 
                245                 250                 255     


Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr 
            260                 265                 270         


Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His Val 
        275                 280                 285             


Ile Ser Tyr Leu Glu 
    290             


<210>  28
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with D197S and Q198A and without N-terminal 
       methionine

<400>  28

Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys 
1               5                   10                  15      


Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly 
            20                  25                  30          


Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val Val 
        35                  40                  45              


Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser 
    50                  55                  60                  


Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly 
65                  70                  75                  80  


Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser 
                85                  90                  95      


Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro 
            100                 105                 110         


Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr Ala 
        115                 120                 125             


Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala Pro 
    130                 135                 140                 


His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe Ser 
145                 150                 155                 160 


Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln 
                165                 170                 175     


Phe Met Ser Pro Ala Ala Asn Asp Leu Asn Val Pro Leu Ser Val Ile 
            180                 185                 190         


Arg Ser Met Ser Ala Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe 
        195                 200                 205             


Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe 
    210                 215                 220                 


Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp Glu 
225                 230                 235                 240 


Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe 
                245                 250                 255     


Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr 
            260                 265                 270         


Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His Val 
        275                 280                 285             


Ile Ser Tyr Leu Glu 
    290             


<210>  29
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with L204Y and without N-terminal methionine

<400>  29

Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys 
1               5                   10                  15      


Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly 
            20                  25                  30          


Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val Val 
        35                  40                  45              


Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser 
    50                  55                  60                  


Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly 
65                  70                  75                  80  


Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser 
                85                  90                  95      


Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro 
            100                 105                 110         


Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr Ala 
        115                 120                 125             


Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala Pro 
    130                 135                 140                 


His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe Ser 
145                 150                 155                 160 


Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln 
                165                 170                 175     


Phe Met Ser Pro Ala Ala Asn Asp Leu Asn Val Pro Leu Ser Val Ile 
            180                 185                 190         


Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Tyr Val Phe Lys Tyr Phe 
        195                 200                 205             


Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe 
    210                 215                 220                 


Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp Glu 
225                 230                 235                 240 


Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe 
                245                 250                 255     


Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr 
            260                 265                 270         


Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His Val 
        275                 280                 285             


Ile Ser Tyr Leu Glu 
    290             


<210>  30
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with F230L and without N-terminal methionine

<400>  30

Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys 
1               5                   10                  15      


Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly 
            20                  25                  30          


Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val Val 
        35                  40                  45              


Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser 
    50                  55                  60                  


Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly 
65                  70                  75                  80  


Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser 
                85                  90                  95      


Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro 
            100                 105                 110         


Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr Ala 
        115                 120                 125             


Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala Pro 
    130                 135                 140                 


His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe Ser 
145                 150                 155                 160 


Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln 
                165                 170                 175     


Phe Met Ser Pro Ala Ala Asn Asp Leu Asn Val Pro Leu Ser Val Ile 
            180                 185                 190         


Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe 
        195                 200                 205             


Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe 
    210                 215                 220                 


Tyr Leu Thr Ile Leu His Pro Ala Ser Val Gly Lys Lys Ala Asp Glu 
225                 230                 235                 240 


Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe 
                245                 250                 255     


Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr 
            260                 265                 270         


Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His Val 
        275                 280                 285             


Ile Ser Tyr Leu Glu 
    290             


<210>  31
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with H231Y and without N-terminal methionine

<400>  31

Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys 
1               5                   10                  15      


Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly 
            20                  25                  30          


Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val Val 
        35                  40                  45              


Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser 
    50                  55                  60                  


Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly 
65                  70                  75                  80  


Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser 
                85                  90                  95      


Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro 
            100                 105                 110         


Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr Ala 
        115                 120                 125             


Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala Pro 
    130                 135                 140                 


His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe Ser 
145                 150                 155                 160 


Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln 
                165                 170                 175     


Phe Met Ser Pro Ala Ala Asn Asp Leu Asn Val Pro Leu Ser Val Ile 
            180                 185                 190         


Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe 
        195                 200                 205             


Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe 
    210                 215                 220                 


Tyr Leu Thr Ile Phe Tyr Pro Ala Ser Val Gly Lys Lys Ala Asp Glu 
225                 230                 235                 240 


Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe 
                245                 250                 255     


Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr 
            260                 265                 270         


Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His Val 
        275                 280                 285             


Ile Ser Tyr Leu Glu 
    290             


<210>  32
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with S234A and without N-terminal methionine

<400>  32

Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys 
1               5                   10                  15      


Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly 
            20                  25                  30          


Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val Val 
        35                  40                  45              


Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser 
    50                  55                  60                  


Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly 
65                  70                  75                  80  


Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser 
                85                  90                  95      


Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro 
            100                 105                 110         


Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr Ala 
        115                 120                 125             


Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala Pro 
    130                 135                 140                 


His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe Ser 
145                 150                 155                 160 


Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln 
                165                 170                 175     


Phe Met Ser Pro Ala Ala Asn Asp Leu Asn Val Pro Leu Ser Val Ile 
            180                 185                 190         


Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe 
        195                 200                 205             


Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe 
    210                 215                 220                 


Tyr Leu Thr Ile Phe His Pro Ala Ala Val Gly Lys Lys Ala Asp Glu 
225                 230                 235                 240 


Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe 
                245                 250                 255     


Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr 
            260                 265                 270         


Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His Val 
        275                 280                 285             


Ile Ser Tyr Leu Glu 
    290             


<210>  33
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with C135S and without N-terminal methionine

<400>  33

Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys 
1               5                   10                  15      


Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly 
            20                  25                  30          


Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val Val 
        35                  40                  45              


Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser 
    50                  55                  60                  


Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly 
65                  70                  75                  80  


Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser 
                85                  90                  95      


Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro 
            100                 105                 110         


Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr Ala 
        115                 120                 125             


Lys Val Lys Asp Trp Ser Gly Val His Val Pro Asn His Arg Ala Pro 
    130                 135                 140                 


His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe Ser 
145                 150                 155                 160 


Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln 
                165                 170                 175     


Phe Met Ser Pro Ala Ala Asn Asp Leu Asn Val Pro Leu Ser Val Ile 
            180                 185                 190         


Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe 
        195                 200                 205             


Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe 
    210                 215                 220                 


Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp Glu 
225                 230                 235                 240 


Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe 
                245                 250                 255     


Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr 
            260                 265                 270         


Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His Val 
        275                 280                 285             


Ile Ser Tyr Leu Glu 
    290             


<210>  34
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with N187G and F230L and without N-terminal 
       methionine

<400>  34

Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys 
1               5                   10                  15      


Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly 
            20                  25                  30          


Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val Val 
        35                  40                  45              


Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser 
    50                  55                  60                  


Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly 
65                  70                  75                  80  


Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser 
                85                  90                  95      


Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro 
            100                 105                 110         


Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr Ala 
        115                 120                 125             


Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala Pro 
    130                 135                 140                 


His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe Ser 
145                 150                 155                 160 


Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln 
                165                 170                 175     


Phe Met Ser Pro Ala Ala Asn Asp Leu Gly Val Pro Leu Ser Val Ile 
            180                 185                 190         


Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe 
        195                 200                 205             


Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe 
    210                 215                 220                 


Tyr Leu Thr Ile Leu His Pro Ala Ser Val Gly Lys Lys Ala Asp Glu 
225                 230                 235                 240 


Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe 
                245                 250                 255     


Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr 
            260                 265                 270         


Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His Val 
        275                 280                 285             


Ile Ser Tyr Leu Glu 
    290             


<210>  35
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with A182T, N187G and F230L and without N-terminal 
       methionine

<400>  35

Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys 
1               5                   10                  15      


Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly 
            20                  25                  30          


Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val Val 
        35                  40                  45              


Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser 
    50                  55                  60                  


Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly 
65                  70                  75                  80  


Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser 
                85                  90                  95      


Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro 
            100                 105                 110         


Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr Ala 
        115                 120                 125             


Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala Pro 
    130                 135                 140                 


His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe Ser 
145                 150                 155                 160 


Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln 
                165                 170                 175     


Phe Met Ser Pro Thr Ala Asn Asp Leu Gly Val Pro Leu Ser Val Ile 
            180                 185                 190         


Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe 
        195                 200                 205             


Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe 
    210                 215                 220                 


Tyr Leu Thr Ile Leu His Pro Ala Ser Val Gly Lys Lys Ala Asp Glu 
225                 230                 235                 240 


Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe 
                245                 250                 255     


Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr 
            260                 265                 270         


Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His Val 
        275                 280                 285             


Ile Ser Tyr Leu Glu 
    290             


<210>  36
<211>  6
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  36

Lys Arg Lys Lys Arg Lys 
1               5       


<210>  37
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  synethtic sequence


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  37

Lys Arg Xaa Lys Arg 
1               5   


<210>  38
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  38

Lys Arg Ser Lys Arg 
1               5   


<210>  39
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  39

Lys Arg Gly Ser Gly 
1               5   


<210>  40
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  40

Lys Arg Lys Lys Arg Lys Lys Arg Lys 
1               5                   


<210>  41
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  41

Arg Arg Arg Arg Arg Arg Arg Arg Arg 
1               5                   


<210>  42
<211>  8
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  42

Lys Lys Lys Lys Lys Lys Lys Lys 
1               5               


<210>  43
<211>  10
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  43

Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys 
1               5                   10  


<210>  44
<211>  12
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  44

Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys 
1               5                   10          


<210>  45
<211>  14
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  45

Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg 
1               5                   10                  


<210>  46
<211>  16
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  46

Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys 
1               5                   10                  15      


<210>  47
<211>  18
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  47

Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys 
1               5                   10                  15      


Arg Lys 
        


<210>  48
<211>  19
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  48

Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys 
1               5                   10                  15      


Arg Lys Lys 
            


<210>  49
<211>  19
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  49

Arg Arg Arg Arg Arg Arg Arg Arg Arg Arg Arg Arg Arg Arg Arg Arg 
1               5                   10                  15      


Arg Arg Arg 
            


<210>  50
<211>  19
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  50

Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys 
1               5                   10                  15      


Lys Lys Lys 
            


<210>  51
<211>  20
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  51

Lys Arg Lys Lys Arg Lys Lys Arg Lys Arg Ser Lys Arg Lys Lys Arg 
1               5                   10                  15      


Lys Lys Arg Lys 
            20  


<210>  52
<211>  21
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  52

Lys Arg Lys Lys Arg Lys Lys Arg Lys Arg Ser Lys Arg Lys Lys Arg 
1               5                   10                  15      


Lys Lys Arg Lys Lys 
            20      


<210>  53
<211>  21
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  53

Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys 
1               5                   10                  15      


Arg Lys Lys Arg Lys 
            20      


<210>  54
<211>  22
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  54

Lys Arg Lys Lys Arg Lys Lys Arg Lys Arg Gly Ser Gly Lys Arg Lys 
1               5                   10                  15      


Lys Arg Lys Lys Arg Lys 
            20          


<210>  55
<211>  24
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  55

Lys Arg Lys Lys Arg Lys Lys Arg Lys Arg Gly Ser Gly Ser Gly Lys 
1               5                   10                  15      


Arg Lys Lys Arg Lys Lys Arg Lys 
            20                  


<210>  56
<211>  25
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  56

Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys 
1               5                   10                  15      


Arg Lys Lys Arg Lys Lys Arg Lys Lys 
            20                  25  


<210>  57
<211>  31
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  57

Lys Arg Lys Lys Arg Lys Lys Arg Lys Arg Ser Lys Arg Lys Lys Arg 
1               5                   10                  15      


Lys Lys Arg Lys Arg Ser Lys Arg Lys Lys Arg Lys Lys Arg Lys 
            20                  25                  30      


<210>  58
<211>  38
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  58

Lys Arg Lys Lys Arg Lys Lys Arg Lys Arg Gly Ser Gly Ser Gly Lys 
1               5                   10                  15      


Arg Lys Lys Arg Lys Lys Arg Lys Gly Ser Gly Ser Gly Lys Arg Lys 
            20                  25                  30          


Lys Arg Lys Lys Arg Lys 
        35              


<210>  59
<211>  39
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  59

Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys 
1               5                   10                  15      


Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg 
            20                  25                  30          


Lys Lys Arg Lys Lys Arg Lys 
        35                  


<210>  60
<211>  42
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  60

Lys Arg Lys Lys Arg Lys Lys Arg Lys Arg Ser Lys Arg Lys Lys Arg 
1               5                   10                  15      


Lys Lys Arg Lys Arg Ser Lys Arg Lys Lys Arg Lys Lys Arg Lys Arg 
            20                  25                  30          


Ser Lys Arg Lys Lys Arg Lys Lys Arg Lys 
        35                  40          


<210>  61
<211>  37
<212>  PRT
<213>  Homo sapiens

<400>  61

Leu Leu Gly Asp Phe Phe Arg Lys Ser Lys Glu Lys Ile Gly Lys Glu 
1               5                   10                  15      


Phe Lys Arg Ile Val Gln Arg Ile Lys Asp Phe Leu Arg Asn Leu Val 
            20                  25                  30          


Pro Arg Thr Glu Ser 
        35          


<210>  62
<211>  29
<212>  PRT
<213>  unknown

<220>
<223>  SMAP-29 sheep

<400>  62

Arg Gly Leu Arg Arg Leu Gly Arg Lys Ile Ala His Gly Val Lys Lys 
1               5                   10                  15      


Tyr Gly Pro Thr Val Leu Arg Ile Ile Arg Ile Ala Gly 
            20                  25                  


<210>  63
<211>  13
<212>  PRT
<213>  unknown

<220>
<223>  Indolicidine bovine

<400>  63

Ile Leu Pro Trp Lys Trp Pro Trp Trp Pro Trp Arg Arg 
1               5                   10              


<210>  64
<211>  18
<212>  PRT
<213>  unknown

<220>
<223>  Protegrin Porcine

<400>  64

Arg Gly Gly Arg Leu Cys Tyr Cys Arg Arg Arg Phe Cys Val Cys Val 
1               5                   10                  15      


Gly Arg 
        


<210>  65
<211>  31
<212>  PRT
<213>  unknown

<220>
<223>  Cecropin P1 Mammal (pig)

<400>  65

Ser Trp Leu Ser Lys Thr Ala Lys Lys Leu Glu Asn Ser Ala Lys Lys 
1               5                   10                  15      


Arg Ile Ser Glu Gly Ile Ala Ile Ala Ile Gln Gly Gly Pro Arg 
            20                  25                  30      


<210>  66
<211>  23
<212>  PRT
<213>  unknown

<220>
<223>  Magainin frog

<400>  66

Gly Ile Gly Lys Phe Leu His Ser Ala Lys Lys Phe Gly Lys Ala Phe 
1               5                   10                  15      


Val Gly Glu Ile Met Asn Ser 
            20              


<210>  67
<211>  25
<212>  PRT
<213>  unknown

<220>
<223>  Pleurocidin fish

<400>  67

Gly Trp Gly Ser Phe Phe Lys Lys Ala Ala His Val Gly Lys His Val 
1               5                   10                  15      


Gly Lys Ala Ala Leu Thr His Tyr Leu 
            20                  25  


<210>  68
<211>  36
<212>  PRT
<213>  Aedes aegypti

<400>  68

Gly Gly Leu Lys Lys Leu Gly Lys Lys Leu Glu Gly Ala Gly Lys Arg 
1               5                   10                  15      


Val Phe Asn Ala Ala Glu Lys Ala Leu Pro Val Val Ala Gly Ala Lys 
            20                  25                  30          


Ala Leu Arg Lys 
        35      


<210>  69
<211>  40
<212>  PRT
<213>  Drosophila melanogaster

<400>  69

Gly Trp Leu Lys Lys Ile Gly Lys Lys Ile Glu Arg Val Gly Gln His 
1               5                   10                  15      


Thr Arg Asp Ala Thr Ile Gln Gly Leu Gly Ile Pro Gln Gln Ala Ala 
            20                  25                  30          


Asn Val Ala Ala Thr Ala Arg Gly 
        35                  40  


<210>  70
<211>  21
<212>  PRT
<213>  unknown

<220>
<223>  Buforin II vertebrate

<400>  70

Thr Arg Ser Ser Arg Ala Gly Leu Gln Phe Pro Val Gly Arg Val His 
1               5                   10                  15      


Arg Leu Leu Arg Lys 
            20      


<210>  71
<211>  39
<212>  PRT
<213>  unknown

<220>
<223>  Sarcotoxin IA Fly

<400>  71

Gly Trp Leu Lys Lys Ile Gly Lys Lys Ile Glu Arg Val Gly Gln His 
1               5                   10                  15      


Thr Arg Asp Ala Thr Ile Gln Gly Leu Gly Ile Ala Gln Gln Ala Ala 
            20                  25                  30          


Asn Val Ala Ala Thr Ala Arg 
        35                  


<210>  72
<211>  17
<212>  PRT
<213>  Apis mellifera

<400>  72

Ala Asn Arg Pro Val Tyr Ile Pro Pro Pro Arg Pro Pro His Pro Arg 
1               5                   10                  15      


Leu 
    


<210>  73
<211>  24
<212>  PRT
<213>  unknown

<220>
<223>  Ascaphine 5 Frog

<400>  73

Gly Ile Lys Asp Trp Ile Lys Gly Ala Ala Lys Lys Leu Ile Lys Thr 
1               5                   10                  15      


Val Ala Ser His Ile Ala Asn Gln 
            20                  


<210>  74
<211>  22
<212>  PRT
<213>  unknown

<220>
<223>  Nigrocine 2 Frog

<400>  74

Gly Leu Leu Ser Lys Val Leu Gly Val Gly Lys Lys Val Leu Cys Gly 
1               5                   10                  15      


Val Ser Gly Leu Val Cys 
            20          


<210>  75
<211>  24
<212>  PRT
<213>  unknown

<220>
<223>  Pseudin 1 Rana Frog

<400>  75

Gly Leu Asn Thr Leu Lys Lys Val Phe Gln Gly Leu His Glu Ala Ile 
1               5                   10                  15      


Lys Leu Ile Asn Asn His Val Gln 
            20                  


<210>  76
<211>  18
<212>  PRT
<213>  unknown

<220>
<223>  Ranalexin Frog

<400>  76

Phe Leu Gly Gly Leu Ile Val Pro Ala Met Ile Cys Ala Val Thr Lys 
1               5                   10                  15      


Lys Cys 
        


<210>  77
<211>  26
<212>  PRT
<213>  unknown

<220>
<223>  Melittin bee

<400>  77

Gly Ile Gly Ala Val Leu Lys Val Leu Thr Thr Gly Leu Pro Ala Leu 
1               5                   10                  15      


Ile Ser Trp Ile Lys Arg Lys Arg Gln Gln 
            20                  25      


<210>  78
<211>  25
<212>  PRT
<213>  unknown

<220>
<223>  Lycotoxin 1 Spider

<400>  78

Ile Trp Leu Thr Ala Leu Lys Phe Leu Gly Lys His Ala Ala Lys Lys 
1               5                   10                  15      


Leu Ala Lys Gln Gln Leu Ser Lys Leu 
            20                  25  


<210>  79
<211>  19
<212>  PRT
<213>  unknown

<220>
<223>  Parasin 1 Fish

<400>  79

Lys Gly Arg Gly Lys Gln Gly Gly Lys Val Arg Ala Lys Ala Lys Thr 
1               5                   10                  15      


Arg Ser Ser 
            


<210>  80
<211>  39
<212>  PRT
<213>  unknown

<220>
<223>  Buforin I Toad

<400>  80

Ala Gly Arg Gly Lys Gln Gly Gly Lys Val Arg Ala Lys Ala Lys Thr 
1               5                   10                  15      


Arg Ser Ser Arg Ala Gly Leu Gln Phe Pro Val Gly Arg Val His Arg 
            20                  25                  30          


Leu Leu Arg Lys Gly Asn Tyr 
        35                  


<210>  81
<211>  34
<212>  PRT
<213>  unknown

<220>
<223>  Dermaseptin 1 Frog

<400>  81

Ala Leu Trp Lys Thr Met Leu Lys Lys Leu Gly Thr Met Ala Leu His 
1               5                   10                  15      


Ala Gly Lys Ala Ala Leu Gly Ala Ala Ala Asp Thr Ile Ser Gln Gly 
            20                  25                  30          


Thr Gln 
        


<210>  82
<211>  12
<212>  PRT
<213>  unknown

<220>
<223>  Bactenecin 1 Cow

<400>  82

Arg Leu Cys Arg Ile Val Val Ile Arg Val Cys Arg 
1               5                   10          


<210>  83
<211>  21
<212>  PRT
<213>  unknown

<220>
<223>  Thanatin Insect

<400>  83

Gly Ser Lys Lys Pro Val Pro Ile Ile Tyr Cys Asn Arg Arg Thr Gly 
1               5                   10                  15      


Lys Cys Gln Arg Met 
            20      


<210>  84
<211>  19
<212>  PRT
<213>  unknown

<220>
<223>  Brevinin 1T Rana frogs

<400>  84

Val Asn Pro Ile Ile Leu Gly Val Leu Pro Lys Val Cys Leu Ile Thr 
1               5                   10                  15      


Lys Lys Cys 
            


<210>  85
<211>  26
<212>  PRT
<213>  unknown

<220>
<223>  Ranateurin 1 Rana frog

<400>  85

Ser Met Leu Ser Val Leu Lys Asn Leu Gly Lys Val Gly Leu Gly Phe 
1               5                   10                  15      


Val Ala Cys Lys Ile Asn Ile Lys Gln Cys 
            20                  25      


<210>  86
<211>  46
<212>  PRT
<213>  unknown

<220>
<223>  Esculentin 1 Rana frogs

<400>  86

Gly Ile Phe Ser Lys Leu Gly Arg Lys Lys Ile Lys Asn Leu Leu Ile 
1               5                   10                  15      


Ser Gly Leu Lys Asn Val Gly Lys Glu Val Gly Met Asp Val Val Arg 
            20                  25                  30          


Thr Gly Ile Lys Ile Ala Gly Cys Lys Ile Lys Gly Glu Cys 
        35                  40                  45      


<210>  87
<211>  17
<212>  PRT
<213>  Limulus polyphemus

<400>  87

Arg Trp Cys Phe Arg Val Cys Tyr Arg Gly Ile Cys Tyr Arg Lys Cys 
1               5                   10                  15      


Arg 
    


<210>  88
<211>  25
<212>  PRT
<213>  unknown

<220>
<223>  Androctonin Scorpion

<400>  88

Arg Ser Val Cys Arg Gln Ile Lys Ile Cys Arg Arg Arg Gly Gly Cys 
1               5                   10                  15      


Tyr Tyr Lys Cys Thr Asn Arg Pro Tyr 
            20                  25  


<210>  89
<211>  30
<212>  PRT
<213>  Homo sapiens

<400>  89

Asp Cys Tyr Cys Arg Ile Pro Ala Cys Ile Ala Gly Glu Arg Arg Tyr 
1               5                   10                  15      


Gly Thr Cys Ile Tyr Gln Gly Arg Leu Trp Ala Phe Cys Cys 
            20                  25                  30  


<210>  90
<211>  38
<212>  PRT
<213>  unknown

<220>
<223>  beta-defensin cow

<400>  90

Asn Pro Val Ser Cys Val Arg Asn Lys Gly Ile Cys Val Pro Ile Arg 
1               5                   10                  15      


Cys Pro Gly Ser Met Lys Gln Ile Gly Thr Cys Val Gly Arg Ala Val 
            20                  25                  30          


Lys Cys Cys Arg Lys Lys 
        35              


<210>  91
<211>  18
<212>  PRT
<213>  unknown

<220>
<223>  theta-defensin monkey

<400>  91

Gly Phe Cys Arg Cys Leu Cys Arg Arg Gly Val Cys Arg Cys Ile Cys 
1               5                   10                  15      


Thr Arg 
        


<210>  92
<211>  40
<212>  PRT
<213>  unknown

<220>
<223>  defensin (sapecin A) insect

<400>  92

Ala Thr Cys Asp Leu Leu Ser Gly Thr Gly Ile Asn His Ser Ala Cys 
1               5                   10                  15      


Ala Ala His Cys Leu Leu Arg Gly Asn Arg Gly Gly Tyr Cys Asn Gly 
            20                  25                  30          


Lys Ala Val Cys Val Cys Arg Asn 
        35                  40  


<210>  93
<211>  46
<212>  PRT
<213>  unknown

<220>
<223>  Thionin (crambin) plant

<400>  93

Thr Thr Cys Cys Pro Ser Ile Val Ala Arg Ser Asn Phe Asn Val Cys 
1               5                   10                  15      


Arg Ile Pro Gly Thr Pro Glu Ala Ile Cys Ala Thr Tyr Thr Gly Cys 
            20                  25                  30          


Ile Ile Ile Pro Gly Ala Thr Cys Pro Gly Asp Tyr Ala Asn 
        35                  40                  45      


<210>  94
<211>  50
<212>  PRT
<213>  unknown

<220>
<223>  defensin from radish

<400>  94

Gln Lys Leu Cys Gln Arg Pro Ser Gly Thr Trp Ser Gly Val Cys Gly 
1               5                   10                  15      


Asn Asn Asn Ala Cys Lys Asn Gln Cys Ile Arg Leu Glu Lys Ala Arg 
            20                  25                  30          


His Gly Ser Cys Asn Tyr Val Phe Pro Ala His Cys Ile Cys Tyr Phe 
        35                  40                  45              


Pro Cys 
    50  


<210>  95
<211>  44
<212>  PRT
<213>  Drosophila melanogaster

<400>  95

Asp Cys Leu Ser Gly Arg Tyr Lys Gly Pro Cys Ala Val Trp Asp Asn 
1               5                   10                  15      


Glu Thr Cys Arg Arg Val Cys Lys Glu Glu Gly Arg Ser Ser Gly His 
            20                  25                  30          


Cys Ser Pro Ser Leu Lys Cys Trp Cys Glu Gly Cys 
        35                  40                  


<210>  96
<211>  25
<212>  PRT
<213>  Homo sapiens

<400>  96

Asp Thr His Phe Pro Ile Cys Ile Phe Cys Cys Gly Cys Cys His Arg 
1               5                   10                  15      


Ser Lys Cys Gly Met Cys Cys Lys Thr 
            20                  25  


<210>  97
<211>  44
<212>  PRT
<213>  unknown

<220>
<223>  Bac 5 Cow

<400>  97

Arg Phe Arg Pro Pro Ile Arg Arg Pro Pro Ile Arg Pro Pro Phe Tyr 
1               5                   10                  15      


Pro Pro Phe Arg Pro Pro Ile Arg Pro Pro Ile Phe Pro Pro Ile Arg 
            20                  25                  30          


Pro Pro Phe Arg Pro Pro Leu Gly Arg Pro Phe Pro 
        35                  40                  


<210>  98
<211>  39
<212>  PRT
<213>  unknown

<220>
<223>  PR-39 Pig

<400>  98

Arg Arg Arg Pro Arg Pro Pro Tyr Leu Pro Arg Pro Arg Pro Pro Pro 
1               5                   10                  15      


Phe Phe Pro Pro Arg Leu Pro Pro Arg Ile Pro Pro Gly Phe Pro Pro 
            20                  25                  30          


Arg Phe Pro Pro Arg Phe Pro 
        35                  


<210>  99
<211>  20
<212>  PRT
<213>  unknown

<220>
<223>  Pyrrhocoricin Insect

<400>  99

Val Asp Lys Gly Ser Tyr Leu Pro Arg Pro Thr Pro Pro Arg Pro Ile 
1               5                   10                  15      


Tyr Asn Arg Asn 
            20  


<210>  100
<211>  24
<212>  PRT
<213>  Homo sapiens

<400>  100

Asp Ser His Ala Lys Arg His His Gly Tyr Lys Arg Lys Phe His Glu 
1               5                   10                  15      


Lys His His Ser His Arg Gly Tyr 
            20                  


<210>  101
<211>  34
<212>  PRT
<213>  Limulus polyphemus

<400>  101

Gly Phe Lys Leu Lys Gly Met Ala Arg Ile Ser Cys Leu Pro Asn Gly 
1               5                   10                  15      


Gln Trp Ser Asn Phe Pro Pro Lys Cys Ile Arg Glu Cys Ala Met Val 
            20                  25                  30          


Ser Ser 
        


<210>  102
<211>  18
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  102

Gly Phe Phe Ile Pro Ala Val Ile Leu Pro Ser Ile Ala Phe Leu Ile 
1               5                   10                  15      


Val Pro 
        


<210>  103
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  103

Phe Phe Val Ala Pro 
1               5   


<210>  104
<211>  13
<212>  PRT
<213>  unknown

<220>
<223>  alpha4-helix of T4 lysozyme

<400>  104

Pro Asn Arg Ala Lys Arg Val Ile Thr Thr Phe Arg Thr 
1               5                   10              


<210>  105
<211>  27
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  105

Lys Arg Trp Val Lys Arg Val Lys Arg Val Lys Arg Trp Val Lys Arg 
1               5                   10                  15      


Val Val Arg Val Val Lys Arg Trp Val Lys Arg 
            20                  25          


<210>  106
<211>  25
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  106

Gly Lys Pro Gly Trp Leu Ile Lys Lys Ala Leu Val Phe Lys Lys Leu 
1               5                   10                  15      


Ile Arg Arg Pro Leu Lys Arg Leu Ala 
            20                  25  


<210>  107
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with S120G plus KRKKRKKRK peptide

<400>  107

Met Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn 
1               5                   10                  15      


Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr 
            20                  25                  30          


Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu 
        35                  40                  45              


Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly 
    50                  55                  60                  


Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr 
65                  70                  75                  80  


Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val 
                85                  90                  95      


Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe 
            100                 105                 110         


Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp 
        115                 120                 125             


Gly Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys 
    130                 135                 140                 


Gly Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys 
145                 150                 155                 160 


Met Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala 
                165                 170                 175     


Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala 
            180                 185                 190         


Asn Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu 
        195                 200                 205             


Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg 
    210                 215                 220                 


Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His 
225                 230                 235                 240 


Pro Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly 
                245                 250                 255     


Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly 
            260                 265                 270         


Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr 
        275                 280                 285             


Lys Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu 
    290                 295                 300             


<210>  108
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with A126E and T128K plus KRKKRKKRK peptide

<400>  108

Met Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn 
1               5                   10                  15      


Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr 
            20                  25                  30          


Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu 
        35                  40                  45              


Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly 
    50                  55                  60                  


Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr 
65                  70                  75                  80  


Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val 
                85                  90                  95      


Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe 
            100                 105                 110         


Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp 
        115                 120                 125             


Ser Gly Lys Val Ser Pro Glu Phe Lys Ala Lys Val Lys Asp Trp Cys 
    130                 135                 140                 


Gly Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys 
145                 150                 155                 160 


Met Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala 
                165                 170                 175     


Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala 
            180                 185                 190         


Asn Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu 
        195                 200                 205             


Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg 
    210                 215                 220                 


Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His 
225                 230                 235                 240 


Pro Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly 
                245                 250                 255     


Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly 
            260                 265                 270         


Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr 
        275                 280                 285             


Lys Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu 
    290                 295                 300             


<210>  109
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with W134I plus KRKKRKKRK peptide

<400>  109

Met Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn 
1               5                   10                  15      


Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr 
            20                  25                  30          


Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu 
        35                  40                  45              


Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly 
    50                  55                  60                  


Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr 
65                  70                  75                  80  


Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val 
                85                  90                  95      


Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe 
            100                 105                 110         


Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp 
        115                 120                 125             


Ser Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Ile Cys 
    130                 135                 140                 


Gly Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys 
145                 150                 155                 160 


Met Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala 
                165                 170                 175     


Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala 
            180                 185                 190         


Asn Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu 
        195                 200                 205             


Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg 
    210                 215                 220                 


Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His 
225                 230                 235                 240 


Pro Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly 
                245                 250                 255     


Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly 
            260                 265                 270         


Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr 
        275                 280                 285             


Lys Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu 
    290                 295                 300             


<210>  110
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with H138L plus KRKKRKKRK peptide

<400>  110

Met Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn 
1               5                   10                  15      


Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr 
            20                  25                  30          


Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu 
        35                  40                  45              


Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly 
    50                  55                  60                  


Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr 
65                  70                  75                  80  


Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val 
                85                  90                  95      


Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe 
            100                 105                 110         


Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp 
        115                 120                 125             


Ser Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys 
    130                 135                 140                 


Gly Val Leu Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys 
145                 150                 155                 160 


Met Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala 
                165                 170                 175     


Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala 
            180                 185                 190         


Asn Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu 
        195                 200                 205             


Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg 
    210                 215                 220                 


Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His 
225                 230                 235                 240 


Pro Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly 
                245                 250                 255     


Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly 
            260                 265                 270         


Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr 
        275                 280                 285             


Lys Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu 
    290                 295                 300             


<210>  111
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with E171G plus KRKKRKKRK peptide

<400>  111

Met Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn 
1               5                   10                  15      


Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr 
            20                  25                  30          


Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu 
        35                  40                  45              


Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly 
    50                  55                  60                  


Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr 
65                  70                  75                  80  


Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val 
                85                  90                  95      


Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe 
            100                 105                 110         


Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp 
        115                 120                 125             


Ser Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys 
    130                 135                 140                 


Gly Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys 
145                 150                 155                 160 


Met Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala 
                165                 170                 175     


Ala Gly Ser Gly Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala 
            180                 185                 190         


Asn Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu 
        195                 200                 205             


Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg 
    210                 215                 220                 


Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His 
225                 230                 235                 240 


Pro Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly 
                245                 250                 255     


Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly 
            260                 265                 270         


Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr 
        275                 280                 285             


Lys Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu 
    290                 295                 300             


<210>  112
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with Y173T plus KRKKRKKRK peptide

<400>  112

Met Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn 
1               5                   10                  15      


Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr 
            20                  25                  30          


Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu 
        35                  40                  45              


Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly 
    50                  55                  60                  


Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr 
65                  70                  75                  80  


Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val 
                85                  90                  95      


Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe 
            100                 105                 110         


Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp 
        115                 120                 125             


Ser Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys 
    130                 135                 140                 


Gly Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys 
145                 150                 155                 160 


Met Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala 
                165                 170                 175     


Ala Gly Ser Glu Ala Thr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala 
            180                 185                 190         


Asn Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu 
        195                 200                 205             


Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg 
    210                 215                 220                 


Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His 
225                 230                 235                 240 


Pro Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly 
                245                 250                 255     


Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly 
            260                 265                 270         


Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr 
        275                 280                 285             


Lys Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu 
    290                 295                 300             


<210>  113
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with A182T plus KRKKRKKRK peptide

<400>  113

Met Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn 
1               5                   10                  15      


Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr 
            20                  25                  30          


Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu 
        35                  40                  45              


Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly 
    50                  55                  60                  


Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr 
65                  70                  75                  80  


Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val 
                85                  90                  95      


Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe 
            100                 105                 110         


Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp 
        115                 120                 125             


Ser Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys 
    130                 135                 140                 


Gly Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys 
145                 150                 155                 160 


Met Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala 
                165                 170                 175     


Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Thr Ala 
            180                 185                 190         


Asn Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu 
        195                 200                 205             


Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg 
    210                 215                 220                 


Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His 
225                 230                 235                 240 


Pro Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly 
                245                 250                 255     


Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly 
            260                 265                 270         


Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr 
        275                 280                 285             


Lys Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu 
    290                 295                 300             


<210>  114
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with N187G plus KRKKRKKRK peptide

<400>  114

Met Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn 
1               5                   10                  15      


Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr 
            20                  25                  30          


Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu 
        35                  40                  45              


Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly 
    50                  55                  60                  


Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr 
65                  70                  75                  80  


Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val 
                85                  90                  95      


Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe 
            100                 105                 110         


Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp 
        115                 120                 125             


Ser Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys 
    130                 135                 140                 


Gly Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys 
145                 150                 155                 160 


Met Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala 
                165                 170                 175     


Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala 
            180                 185                 190         


Asn Asp Leu Gly Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu 
        195                 200                 205             


Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg 
    210                 215                 220                 


Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His 
225                 230                 235                 240 


Pro Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly 
                245                 250                 255     


Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly 
            260                 265                 270         


Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr 
        275                 280                 285             


Lys Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu 
    290                 295                 300             


<210>  115
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with D197S and Q198A plus KRKKRKKRK peptide

<400>  115

Met Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn 
1               5                   10                  15      


Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr 
            20                  25                  30          


Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu 
        35                  40                  45              


Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly 
    50                  55                  60                  


Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr 
65                  70                  75                  80  


Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val 
                85                  90                  95      


Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe 
            100                 105                 110         


Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp 
        115                 120                 125             


Ser Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys 
    130                 135                 140                 


Gly Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys 
145                 150                 155                 160 


Met Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala 
                165                 170                 175     


Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala 
            180                 185                 190         


Asn Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Ser Ala Leu 
        195                 200                 205             


Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg 
    210                 215                 220                 


Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His 
225                 230                 235                 240 


Pro Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly 
                245                 250                 255     


Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly 
            260                 265                 270         


Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr 
        275                 280                 285             


Lys Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu 
    290                 295                 300             


<210>  116
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with L204Y plus KRKKRKKRK peptide

<400>  116

Met Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn 
1               5                   10                  15      


Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr 
            20                  25                  30          


Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu 
        35                  40                  45              


Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly 
    50                  55                  60                  


Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr 
65                  70                  75                  80  


Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val 
                85                  90                  95      


Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe 
            100                 105                 110         


Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp 
        115                 120                 125             


Ser Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys 
    130                 135                 140                 


Gly Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys 
145                 150                 155                 160 


Met Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala 
                165                 170                 175     


Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala 
            180                 185                 190         


Asn Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu 
        195                 200                 205             


Thr Gln Leu Asp Tyr Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg 
    210                 215                 220                 


Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His 
225                 230                 235                 240 


Pro Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly 
                245                 250                 255     


Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly 
            260                 265                 270         


Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr 
        275                 280                 285             


Lys Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu 
    290                 295                 300             


<210>  117
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with F230L plus KRKKRKKRK peptide

<400>  117

Met Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn 
1               5                   10                  15      


Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr 
            20                  25                  30          


Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu 
        35                  40                  45              


Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly 
    50                  55                  60                  


Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr 
65                  70                  75                  80  


Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val 
                85                  90                  95      


Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe 
            100                 105                 110         


Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp 
        115                 120                 125             


Ser Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys 
    130                 135                 140                 


Gly Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys 
145                 150                 155                 160 


Met Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala 
                165                 170                 175     


Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala 
            180                 185                 190         


Asn Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu 
        195                 200                 205             


Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg 
    210                 215                 220                 


Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Leu His 
225                 230                 235                 240 


Pro Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly 
                245                 250                 255     


Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly 
            260                 265                 270         


Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr 
        275                 280                 285             


Lys Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu 
    290                 295                 300             


<210>  118
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with H231Y plus KRKKRKKRK peptide

<400>  118

Met Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn 
1               5                   10                  15      


Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr 
            20                  25                  30          


Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu 
        35                  40                  45              


Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly 
    50                  55                  60                  


Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr 
65                  70                  75                  80  


Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val 
                85                  90                  95      


Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe 
            100                 105                 110         


Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp 
        115                 120                 125             


Ser Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys 
    130                 135                 140                 


Gly Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys 
145                 150                 155                 160 


Met Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala 
                165                 170                 175     


Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala 
            180                 185                 190         


Asn Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu 
        195                 200                 205             


Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg 
    210                 215                 220                 


Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe Tyr 
225                 230                 235                 240 


Pro Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly 
                245                 250                 255     


Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly 
            260                 265                 270         


Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr 
        275                 280                 285             


Lys Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu 
    290                 295                 300             


<210>  119
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with S234A plus KRKKRKKRK peptide

<400>  119

Met Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn 
1               5                   10                  15      


Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr 
            20                  25                  30          


Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu 
        35                  40                  45              


Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly 
    50                  55                  60                  


Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr 
65                  70                  75                  80  


Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val 
                85                  90                  95      


Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe 
            100                 105                 110         


Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp 
        115                 120                 125             


Ser Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys 
    130                 135                 140                 


Gly Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys 
145                 150                 155                 160 


Met Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala 
                165                 170                 175     


Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala 
            180                 185                 190         


Asn Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu 
        195                 200                 205             


Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg 
    210                 215                 220                 


Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His 
225                 230                 235                 240 


Pro Ala Ala Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly 
                245                 250                 255     


Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly 
            260                 265                 270         


Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr 
        275                 280                 285             


Lys Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu 
    290                 295                 300             


<210>  120
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with C135S plus KRKKRKKRK peptide

<400>  120

Met Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn 
1               5                   10                  15      


Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr 
            20                  25                  30          


Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu 
        35                  40                  45              


Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly 
    50                  55                  60                  


Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr 
65                  70                  75                  80  


Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val 
                85                  90                  95      


Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe 
            100                 105                 110         


Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp 
        115                 120                 125             


Ser Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Ser 
    130                 135                 140                 


Gly Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys 
145                 150                 155                 160 


Met Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala 
                165                 170                 175     


Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala 
            180                 185                 190         


Asn Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu 
        195                 200                 205             


Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg 
    210                 215                 220                 


Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His 
225                 230                 235                 240 


Pro Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly 
                245                 250                 255     


Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly 
            260                 265                 270         


Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr 
        275                 280                 285             


Lys Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu 
    290                 295                 300             


<210>  121
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with A182T plus SMAP peptide

<400>  121

Met Arg Gly Leu Arg Arg Leu Gly Arg Lys Ile Ala His Gly Val Lys 
1               5                   10                  15      


Lys Tyr Gly Pro Thr Val Leu Arg Ile Ile Arg Ile Ala Gly Gly Ser 
            20                  25                  30          


Met Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val 
        35                  40                  45              


Lys Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys 
    50                  55                  60                  


Gly Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val 
65                  70                  75                  80  


Val Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala 
                85                  90                  95      


Ser Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu 
            100                 105                 110         


Gly Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu 
        115                 120                 125             


Ser Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr 
    130                 135                 140                 


Pro Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr 
145                 150                 155                 160 


Ala Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala 
                165                 170                 175     


Pro His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe 
            180                 185                 190         


Ser Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile 
        195                 200                 205             


Gln Phe Met Ser Pro Thr Ala Asn Asp Leu Asn Val Pro Leu Ser Val 
    210                 215                 220                 


Ile Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr 
225                 230                 235                 240 


Phe Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp 
                245                 250                 255     


Phe Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp 
            260                 265                 270         


Glu Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly 
        275                 280                 285             


Phe Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser 
    290                 295                 300                 


Thr Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His 
305                 310                 315                 320 


Val Ile Ser Tyr Leu Glu 
                325     


<210>  122
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with N187G plus SMAP peptide

<400>  122

Met Arg Gly Leu Arg Arg Leu Gly Arg Lys Ile Ala His Gly Val Lys 
1               5                   10                  15      


Lys Tyr Gly Pro Thr Val Leu Arg Ile Ile Arg Ile Ala Gly Gly Ser 
            20                  25                  30          


Met Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val 
        35                  40                  45              


Lys Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys 
    50                  55                  60                  


Gly Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val 
65                  70                  75                  80  


Val Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala 
                85                  90                  95      


Ser Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu 
            100                 105                 110         


Gly Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu 
        115                 120                 125             


Ser Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr 
    130                 135                 140                 


Pro Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr 
145                 150                 155                 160 


Ala Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala 
                165                 170                 175     


Pro His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe 
            180                 185                 190         


Ser Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile 
        195                 200                 205             


Gln Phe Met Ser Pro Ala Ala Asn Asp Leu Gly Val Pro Leu Ser Val 
    210                 215                 220                 


Ile Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr 
225                 230                 235                 240 


Phe Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp 
                245                 250                 255     


Phe Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp 
            260                 265                 270         


Glu Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly 
        275                 280                 285             


Phe Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser 
    290                 295                 300                 


Thr Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His 
305                 310                 315                 320 


Val Ile Ser Tyr Leu Glu 
                325     


<210>  123
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with N187G and F230L plus SMAP peptide

<400>  123

Met Arg Gly Leu Arg Arg Leu Gly Arg Lys Ile Ala His Gly Val Lys 
1               5                   10                  15      


Lys Tyr Gly Pro Thr Val Leu Arg Ile Ile Arg Ile Ala Gly Gly Ser 
            20                  25                  30          


Met Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val 
        35                  40                  45              


Lys Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys 
    50                  55                  60                  


Gly Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val 
65                  70                  75                  80  


Val Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala 
                85                  90                  95      


Ser Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu 
            100                 105                 110         


Gly Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu 
        115                 120                 125             


Ser Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr 
    130                 135                 140                 


Pro Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr 
145                 150                 155                 160 


Ala Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala 
                165                 170                 175     


Pro His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe 
            180                 185                 190         


Ser Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile 
        195                 200                 205             


Gln Phe Met Ser Pro Ala Ala Asn Asp Leu Gly Val Pro Leu Ser Val 
    210                 215                 220                 


Ile Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr 
225                 230                 235                 240 


Phe Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp 
                245                 250                 255     


Phe Tyr Leu Thr Ile Leu His Pro Ala Ser Val Gly Lys Lys Ala Asp 
            260                 265                 270         


Glu Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly 
        275                 280                 285             


Phe Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser 
    290                 295                 300                 


Thr Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His 
305                 310                 315                 320 


Val Ile Ser Tyr Leu Glu 
                325     


<210>  124
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with A182T, N187G and F230L plus SMAP peptide

<400>  124

Met Arg Gly Leu Arg Arg Leu Gly Arg Lys Ile Ala His Gly Val Lys 
1               5                   10                  15      


Lys Tyr Gly Pro Thr Val Leu Arg Ile Ile Arg Ile Ala Gly Gly Ser 
            20                  25                  30          


Met Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val 
        35                  40                  45              


Lys Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys 
    50                  55                  60                  


Gly Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val 
65                  70                  75                  80  


Val Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala 
                85                  90                  95      


Ser Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu 
            100                 105                 110         


Gly Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu 
        115                 120                 125             


Ser Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr 
    130                 135                 140                 


Pro Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr 
145                 150                 155                 160 


Ala Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala 
                165                 170                 175     


Pro His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe 
            180                 185                 190         


Ser Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile 
        195                 200                 205             


Gln Phe Met Ser Pro Thr Ala Asn Asp Leu Gly Val Pro Leu Ser Val 
    210                 215                 220                 


Ile Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr 
225                 230                 235                 240 


Phe Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp 
                245                 250                 255     


Phe Tyr Leu Thr Ile Leu His Pro Ala Ser Val Gly Lys Lys Ala Asp 
            260                 265                 270         


Glu Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly 
        275                 280                 285             


Phe Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser 
    290                 295                 300                 


Thr Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His 
305                 310                 315                 320 


Val Ile Ser Tyr Leu Glu 
                325     


<210>  125
<211>  302
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with S120G plus KRKKRKKRK peptide, without 
       N-terminal methionine

<400>  125

Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn Gly 
1               5                   10                  15      


Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr Gly 
            20                  25                  30          


Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu Thr 
        35                  40                  45              


Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly Ile 
    50                  55                  60                  


Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr Ala 
65                  70                  75                  80  


Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val Asp 
                85                  90                  95      


Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe Glu 
            100                 105                 110         


Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp Gly 
        115                 120                 125             


Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys Gly 
    130                 135                 140                 


Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys Met 
145                 150                 155                 160 


Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala Ala 
                165                 170                 175     


Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala Asn 
            180                 185                 190         


Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu Thr 
        195                 200                 205             


Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg Gly 
    210                 215                 220                 


Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His Pro 
225                 230                 235                 240 


Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly Ser 
                245                 250                 255     


Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly Lys 
            260                 265                 270         


Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr Lys 
        275                 280                 285             


Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu 
    290                 295                 300         


<210>  126
<211>  302
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with A126E and T128K plus KRKKRKKRK peptide, 
       without N-terminal methionine

<400>  126

Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn Gly 
1               5                   10                  15      


Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr Gly 
            20                  25                  30          


Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu Thr 
        35                  40                  45              


Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly Ile 
    50                  55                  60                  


Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr Ala 
65                  70                  75                  80  


Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val Asp 
                85                  90                  95      


Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe Glu 
            100                 105                 110         


Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp Ser 
        115                 120                 125             


Gly Lys Val Ser Pro Glu Phe Lys Ala Lys Val Lys Asp Trp Cys Gly 
    130                 135                 140                 


Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys Met 
145                 150                 155                 160 


Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala Ala 
                165                 170                 175     


Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala Asn 
            180                 185                 190         


Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu Thr 
        195                 200                 205             


Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg Gly 
    210                 215                 220                 


Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His Pro 
225                 230                 235                 240 


Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly Ser 
                245                 250                 255     


Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly Lys 
            260                 265                 270         


Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr Lys 
        275                 280                 285             


Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu 
    290                 295                 300         


<210>  127
<211>  302
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with W134I plus KRKKRKKRK peptide, without 
       N-terminal methionine

<400>  127

Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn Gly 
1               5                   10                  15      


Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr Gly 
            20                  25                  30          


Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu Thr 
        35                  40                  45              


Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly Ile 
    50                  55                  60                  


Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr Ala 
65                  70                  75                  80  


Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val Asp 
                85                  90                  95      


Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe Glu 
            100                 105                 110         


Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp Ser 
        115                 120                 125             


Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Ile Cys Gly 
    130                 135                 140                 


Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys Met 
145                 150                 155                 160 


Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala Ala 
                165                 170                 175     


Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala Asn 
            180                 185                 190         


Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu Thr 
        195                 200                 205             


Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg Gly 
    210                 215                 220                 


Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His Pro 
225                 230                 235                 240 


Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly Ser 
                245                 250                 255     


Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly Lys 
            260                 265                 270         


Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr Lys 
        275                 280                 285             


Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu 
    290                 295                 300         


<210>  128
<211>  302
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with H138L plus KRKKRKKRK peptide, without 
       N-terminal methionine

<400>  128

Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn Gly 
1               5                   10                  15      


Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr Gly 
            20                  25                  30          


Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu Thr 
        35                  40                  45              


Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly Ile 
    50                  55                  60                  


Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr Ala 
65                  70                  75                  80  


Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val Asp 
                85                  90                  95      


Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe Glu 
            100                 105                 110         


Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp Ser 
        115                 120                 125             


Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys Gly 
    130                 135                 140                 


Val Leu Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys Met 
145                 150                 155                 160 


Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala Ala 
                165                 170                 175     


Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala Asn 
            180                 185                 190         


Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu Thr 
        195                 200                 205             


Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg Gly 
    210                 215                 220                 


Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His Pro 
225                 230                 235                 240 


Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly Ser 
                245                 250                 255     


Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly Lys 
            260                 265                 270         


Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr Lys 
        275                 280                 285             


Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu 
    290                 295                 300         


<210>  129
<211>  302
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with E171G plus KRKKRKKRK peptide, without 
       N-terminal methionine

<400>  129

Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn Gly 
1               5                   10                  15      


Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr Gly 
            20                  25                  30          


Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu Thr 
        35                  40                  45              


Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly Ile 
    50                  55                  60                  


Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr Ala 
65                  70                  75                  80  


Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val Asp 
                85                  90                  95      


Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe Glu 
            100                 105                 110         


Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp Ser 
        115                 120                 125             


Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys Gly 
    130                 135                 140                 


Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys Met 
145                 150                 155                 160 


Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala Ala 
                165                 170                 175     


Gly Ser Gly Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala Asn 
            180                 185                 190         


Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu Thr 
        195                 200                 205             


Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg Gly 
    210                 215                 220                 


Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His Pro 
225                 230                 235                 240 


Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly Ser 
                245                 250                 255     


Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly Lys 
            260                 265                 270         


Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr Lys 
        275                 280                 285             


Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu 
    290                 295                 300         


<210>  130
<211>  302
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with Y173T plus KRKKRKKRK peptide, without 
       N-terminal methionine

<400>  130

Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn Gly 
1               5                   10                  15      


Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr Gly 
            20                  25                  30          


Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu Thr 
        35                  40                  45              


Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly Ile 
    50                  55                  60                  


Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr Ala 
65                  70                  75                  80  


Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val Asp 
                85                  90                  95      


Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe Glu 
            100                 105                 110         


Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp Ser 
        115                 120                 125             


Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys Gly 
    130                 135                 140                 


Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys Met 
145                 150                 155                 160 


Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala Ala 
                165                 170                 175     


Gly Ser Glu Ala Thr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala Asn 
            180                 185                 190         


Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu Thr 
        195                 200                 205             


Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg Gly 
    210                 215                 220                 


Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His Pro 
225                 230                 235                 240 


Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly Ser 
                245                 250                 255     


Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly Lys 
            260                 265                 270         


Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr Lys 
        275                 280                 285             


Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu 
    290                 295                 300         


<210>  131
<211>  302
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with A182T plus KRKKRKKRK peptide, without 
       N-terminal methionine

<400>  131

Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn Gly 
1               5                   10                  15      


Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr Gly 
            20                  25                  30          


Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu Thr 
        35                  40                  45              


Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly Ile 
    50                  55                  60                  


Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr Ala 
65                  70                  75                  80  


Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val Asp 
                85                  90                  95      


Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe Glu 
            100                 105                 110         


Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp Ser 
        115                 120                 125             


Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys Gly 
    130                 135                 140                 


Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys Met 
145                 150                 155                 160 


Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala Ala 
                165                 170                 175     


Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Thr Ala Asn 
            180                 185                 190         


Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu Thr 
        195                 200                 205             


Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg Gly 
    210                 215                 220                 


Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His Pro 
225                 230                 235                 240 


Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly Ser 
                245                 250                 255     


Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly Lys 
            260                 265                 270         


Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr Lys 
        275                 280                 285             


Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu 
    290                 295                 300         


<210>  132
<211>  302
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with N187G plus KRKKRKKRK peptide, without 
       N-terminal methionine

<400>  132

Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn Gly 
1               5                   10                  15      


Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr Gly 
            20                  25                  30          


Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu Thr 
        35                  40                  45              


Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly Ile 
    50                  55                  60                  


Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr Ala 
65                  70                  75                  80  


Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val Asp 
                85                  90                  95      


Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe Glu 
            100                 105                 110         


Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp Ser 
        115                 120                 125             


Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys Gly 
    130                 135                 140                 


Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys Met 
145                 150                 155                 160 


Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala Ala 
                165                 170                 175     


Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala Asn 
            180                 185                 190         


Asp Leu Gly Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu Thr 
        195                 200                 205             


Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg Gly 
    210                 215                 220                 


Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His Pro 
225                 230                 235                 240 


Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly Ser 
                245                 250                 255     


Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly Lys 
            260                 265                 270         


Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr Lys 
        275                 280                 285             


Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu 
    290                 295                 300         


<210>  133
<211>  302
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with D197S and Q198A plus KRKKRKKRK peptide, 
       without N-terminal methionine

<400>  133

Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn Gly 
1               5                   10                  15      


Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr Gly 
            20                  25                  30          


Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu Thr 
        35                  40                  45              


Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly Ile 
    50                  55                  60                  


Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr Ala 
65                  70                  75                  80  


Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val Asp 
                85                  90                  95      


Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe Glu 
            100                 105                 110         


Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp Ser 
        115                 120                 125             


Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys Gly 
    130                 135                 140                 


Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys Met 
145                 150                 155                 160 


Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala Ala 
                165                 170                 175     


Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala Asn 
            180                 185                 190         


Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Ser Ala Leu Thr 
        195                 200                 205             


Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg Gly 
    210                 215                 220                 


Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His Pro 
225                 230                 235                 240 


Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly Ser 
                245                 250                 255     


Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly Lys 
            260                 265                 270         


Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr Lys 
        275                 280                 285             


Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu 
    290                 295                 300         


<210>  134
<211>  302
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with L204Y plus KRKKRKKRK peptide, without 
       N-terminal methionine

<400>  134

Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn Gly 
1               5                   10                  15      


Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr Gly 
            20                  25                  30          


Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu Thr 
        35                  40                  45              


Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly Ile 
    50                  55                  60                  


Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr Ala 
65                  70                  75                  80  


Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val Asp 
                85                  90                  95      


Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe Glu 
            100                 105                 110         


Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp Ser 
        115                 120                 125             


Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys Gly 
    130                 135                 140                 


Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys Met 
145                 150                 155                 160 


Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala Ala 
                165                 170                 175     


Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala Asn 
            180                 185                 190         


Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu Thr 
        195                 200                 205             


Gln Leu Asp Tyr Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg Gly 
    210                 215                 220                 


Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His Pro 
225                 230                 235                 240 


Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly Ser 
                245                 250                 255     


Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly Lys 
            260                 265                 270         


Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr Lys 
        275                 280                 285             


Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu 
    290                 295                 300         


<210>  135
<211>  302
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with F230L plus KRKKRKKRK peptide, without 
       N-terminal methionine

<400>  135

Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn Gly 
1               5                   10                  15      


Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr Gly 
            20                  25                  30          


Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu Thr 
        35                  40                  45              


Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly Ile 
    50                  55                  60                  


Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr Ala 
65                  70                  75                  80  


Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val Asp 
                85                  90                  95      


Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe Glu 
            100                 105                 110         


Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp Ser 
        115                 120                 125             


Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys Gly 
    130                 135                 140                 


Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys Met 
145                 150                 155                 160 


Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala Ala 
                165                 170                 175     


Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala Asn 
            180                 185                 190         


Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu Thr 
        195                 200                 205             


Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg Gly 
    210                 215                 220                 


Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Leu His Pro 
225                 230                 235                 240 


Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly Ser 
                245                 250                 255     


Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly Lys 
            260                 265                 270         


Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr Lys 
        275                 280                 285             


Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu 
    290                 295                 300         


<210>  136
<211>  302
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with H231Y plus KRKKRKKRK peptide, without 
       N-terminal methionine

<400>  136

Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn Gly 
1               5                   10                  15      


Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr Gly 
            20                  25                  30          


Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu Thr 
        35                  40                  45              


Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly Ile 
    50                  55                  60                  


Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr Ala 
65                  70                  75                  80  


Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val Asp 
                85                  90                  95      


Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe Glu 
            100                 105                 110         


Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp Ser 
        115                 120                 125             


Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys Gly 
    130                 135                 140                 


Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys Met 
145                 150                 155                 160 


Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala Ala 
                165                 170                 175     


Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala Asn 
            180                 185                 190         


Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu Thr 
        195                 200                 205             


Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg Gly 
    210                 215                 220                 


Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe Tyr Pro 
225                 230                 235                 240 


Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly Ser 
                245                 250                 255     


Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly Lys 
            260                 265                 270         


Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr Lys 
        275                 280                 285             


Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu 
    290                 295                 300         


<210>  137
<211>  302
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with S234A plus KRKKRKKRK peptide, without 
       N-terminal methionine

<400>  137

Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn Gly 
1               5                   10                  15      


Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr Gly 
            20                  25                  30          


Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu Thr 
        35                  40                  45              


Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly Ile 
    50                  55                  60                  


Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr Ala 
65                  70                  75                  80  


Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val Asp 
                85                  90                  95      


Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe Glu 
            100                 105                 110         


Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp Ser 
        115                 120                 125             


Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys Gly 
    130                 135                 140                 


Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys Met 
145                 150                 155                 160 


Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala Ala 
                165                 170                 175     


Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala Asn 
            180                 185                 190         


Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu Thr 
        195                 200                 205             


Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg Gly 
    210                 215                 220                 


Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His Pro 
225                 230                 235                 240 


Ala Ala Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly Ser 
                245                 250                 255     


Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly Lys 
            260                 265                 270         


Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr Lys 
        275                 280                 285             


Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu 
    290                 295                 300         


<210>  138
<211>  302
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with C135S plus KRKKRKKRK peptide, without 
       N-terminal methionine

<400>  138

Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn Gly 
1               5                   10                  15      


Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr Gly 
            20                  25                  30          


Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu Thr 
        35                  40                  45              


Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly Ile 
    50                  55                  60                  


Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr Ala 
65                  70                  75                  80  


Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val Asp 
                85                  90                  95      


Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe Glu 
            100                 105                 110         


Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp Ser 
        115                 120                 125             


Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Ser Gly 
    130                 135                 140                 


Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys Met 
145                 150                 155                 160 


Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala Ala 
                165                 170                 175     


Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala Asn 
            180                 185                 190         


Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu Thr 
        195                 200                 205             


Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg Gly 
    210                 215                 220                 


Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His Pro 
225                 230                 235                 240 


Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly Ser 
                245                 250                 255     


Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly Lys 
            260                 265                 270         


Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr Lys 
        275                 280                 285             


Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu 
    290                 295                 300         


<210>  139
<211>  325
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with A182T plus SMAP peptide, without N-terminal 
       methionine

<400>  139

Arg Gly Leu Arg Arg Leu Gly Arg Lys Ile Ala His Gly Val Lys Lys 
1               5                   10                  15      


Tyr Gly Pro Thr Val Leu Arg Ile Ile Arg Ile Ala Gly Gly Ser Met 
            20                  25                  30          


Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys 
        35                  40                  45              


Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly 
    50                  55                  60                  


Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val Val 
65                  70                  75                  80  


Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser 
                85                  90                  95      


Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly 
            100                 105                 110         


Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser 
        115                 120                 125             


Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro 
    130                 135                 140                 


Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr Ala 
145                 150                 155                 160 


Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala Pro 
                165                 170                 175     


His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe Ser 
            180                 185                 190         


Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln 
        195                 200                 205             


Phe Met Ser Pro Thr Ala Asn Asp Leu Asn Val Pro Leu Ser Val Ile 
    210                 215                 220                 


Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe 
225                 230                 235                 240 


Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe 
                245                 250                 255     


Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp Glu 
            260                 265                 270         


Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe 
        275                 280                 285             


Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr 
    290                 295                 300                 


Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His Val 
305                 310                 315                 320 


Ile Ser Tyr Leu Glu 
                325 


<210>  140
<211>  325
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with N187G plus SMAP peptide, without N-terminal 
       methionine

<400>  140

Arg Gly Leu Arg Arg Leu Gly Arg Lys Ile Ala His Gly Val Lys Lys 
1               5                   10                  15      


Tyr Gly Pro Thr Val Leu Arg Ile Ile Arg Ile Ala Gly Gly Ser Met 
            20                  25                  30          


Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys 
        35                  40                  45              


Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly 
    50                  55                  60                  


Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val Val 
65                  70                  75                  80  


Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser 
                85                  90                  95      


Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly 
            100                 105                 110         


Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser 
        115                 120                 125             


Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro 
    130                 135                 140                 


Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr Ala 
145                 150                 155                 160 


Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala Pro 
                165                 170                 175     


His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe Ser 
            180                 185                 190         


Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln 
        195                 200                 205             


Phe Met Ser Pro Ala Ala Asn Asp Leu Gly Val Pro Leu Ser Val Ile 
    210                 215                 220                 


Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe 
225                 230                 235                 240 


Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe 
                245                 250                 255     


Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp Glu 
            260                 265                 270         


Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe 
        275                 280                 285             


Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr 
    290                 295                 300                 


Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His Val 
305                 310                 315                 320 


Ile Ser Tyr Leu Glu 
                325 


<210>  141
<211>  325
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with N187G and F230L plus SMAP peptide, without 
       N-terminal methionine

<400>  141

Arg Gly Leu Arg Arg Leu Gly Arg Lys Ile Ala His Gly Val Lys Lys 
1               5                   10                  15      


Tyr Gly Pro Thr Val Leu Arg Ile Ile Arg Ile Ala Gly Gly Ser Met 
            20                  25                  30          


Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys 
        35                  40                  45              


Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly 
    50                  55                  60                  


Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val Val 
65                  70                  75                  80  


Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser 
                85                  90                  95      


Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly 
            100                 105                 110         


Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser 
        115                 120                 125             


Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro 
    130                 135                 140                 


Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr Ala 
145                 150                 155                 160 


Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala Pro 
                165                 170                 175     


His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe Ser 
            180                 185                 190         


Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln 
        195                 200                 205             


Phe Met Ser Pro Ala Ala Asn Asp Leu Gly Val Pro Leu Ser Val Ile 
    210                 215                 220                 


Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe 
225                 230                 235                 240 


Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe 
                245                 250                 255     


Tyr Leu Thr Ile Leu His Pro Ala Ser Val Gly Lys Lys Ala Asp Glu 
            260                 265                 270         


Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe 
        275                 280                 285             


Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr 
    290                 295                 300                 


Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His Val 
305                 310                 315                 320 


Ile Ser Tyr Leu Glu 
                325 


<210>  142
<211>  325
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with A182T, N187G and F230 L plus SMAP peptide, 
       without N-terminal methionine

<400>  142

Arg Gly Leu Arg Arg Leu Gly Arg Lys Ile Ala His Gly Val Lys Lys 
1               5                   10                  15      


Tyr Gly Pro Thr Val Leu Arg Ile Ile Arg Ile Ala Gly Gly Ser Met 
            20                  25                  30          


Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys 
        35                  40                  45              


Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly 
    50                  55                  60                  


Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val Val 
65                  70                  75                  80  


Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser 
                85                  90                  95      


Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly 
            100                 105                 110         


Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser 
        115                 120                 125             


Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro 
    130                 135                 140                 


Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr Ala 
145                 150                 155                 160 


Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala Pro 
                165                 170                 175     


His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe Ser 
            180                 185                 190         


Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln 
        195                 200                 205             


Phe Met Ser Pro Thr Ala Asn Asp Leu Gly Val Pro Leu Ser Val Ile 
    210                 215                 220                 


Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe 
225                 230                 235                 240 


Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe 
                245                 250                 255     


Tyr Leu Thr Ile Leu His Pro Ala Ser Val Gly Lys Lys Ala Asp Glu 
            260                 265                 270         


Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe 
        275                 280                 285             


Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr 
    290                 295                 300                 


Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His Val 
305                 310                 315                 320 


Ile Ser Tyr Leu Glu 
                325 


<210>  143
<211>  6
<212>  PRT
<213>  artificial sequence

<220>
<223>  His-Tag (6x)

<400>  143

His His His His His His 
1               5       


<210>  144
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with S120G plus KRKKRKKRK peptide plus HIS-tag

<400>  144

Met Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn 
1               5                   10                  15      


Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr 
            20                  25                  30          


Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu 
        35                  40                  45              


Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly 
    50                  55                  60                  


Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr 
65                  70                  75                  80  


Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val 
                85                  90                  95      


Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe 
            100                 105                 110         


Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp 
        115                 120                 125             


Gly Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys 
    130                 135                 140                 


Gly Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys 
145                 150                 155                 160 


Met Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala 
                165                 170                 175     


Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala 
            180                 185                 190         


Asn Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu 
        195                 200                 205             


Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg 
    210                 215                 220                 


Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His 
225                 230                 235                 240 


Pro Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly 
                245                 250                 255     


Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly 
            260                 265                 270         


Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr 
        275                 280                 285             


Lys Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His 
    290                 295                 300                 


His His His His His 
305                 


<210>  145
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with A126E and T128K plus KRKKRKKRK peptide plus 
       HIS-tag

<400>  145

Met Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn 
1               5                   10                  15      


Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr 
            20                  25                  30          


Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu 
        35                  40                  45              


Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly 
    50                  55                  60                  


Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr 
65                  70                  75                  80  


Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val 
                85                  90                  95      


Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe 
            100                 105                 110         


Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp 
        115                 120                 125             


Ser Gly Lys Val Ser Pro Glu Phe Lys Ala Lys Val Lys Asp Trp Cys 
    130                 135                 140                 


Gly Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys 
145                 150                 155                 160 


Met Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala 
                165                 170                 175     


Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala 
            180                 185                 190         


Asn Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu 
        195                 200                 205             


Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg 
    210                 215                 220                 


Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His 
225                 230                 235                 240 


Pro Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly 
                245                 250                 255     


Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly 
            260                 265                 270         


Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr 
        275                 280                 285             


Lys Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His 
    290                 295                 300                 


His His His His His 
305                 


<210>  146
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with W134I plus KRKKRKKRK peptide plus HIS-tag

<400>  146

Met Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn 
1               5                   10                  15      


Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr 
            20                  25                  30          


Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu 
        35                  40                  45              


Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly 
    50                  55                  60                  


Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr 
65                  70                  75                  80  


Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val 
                85                  90                  95      


Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe 
            100                 105                 110         


Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp 
        115                 120                 125             


Ser Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Ile Cys 
    130                 135                 140                 


Gly Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys 
145                 150                 155                 160 


Met Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala 
                165                 170                 175     


Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala 
            180                 185                 190         


Asn Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu 
        195                 200                 205             


Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg 
    210                 215                 220                 


Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His 
225                 230                 235                 240 


Pro Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly 
                245                 250                 255     


Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly 
            260                 265                 270         


Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr 
        275                 280                 285             


Lys Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His 
    290                 295                 300                 


His His His His His 
305                 


<210>  147
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with H138L plus KRKKRKKRK peptide plus HIS-tag

<400>  147

Met Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn 
1               5                   10                  15      


Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr 
            20                  25                  30          


Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu 
        35                  40                  45              


Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly 
    50                  55                  60                  


Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr 
65                  70                  75                  80  


Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val 
                85                  90                  95      


Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe 
            100                 105                 110         


Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp 
        115                 120                 125             


Ser Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys 
    130                 135                 140                 


Gly Val Leu Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys 
145                 150                 155                 160 


Met Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala 
                165                 170                 175     


Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala 
            180                 185                 190         


Asn Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu 
        195                 200                 205             


Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg 
    210                 215                 220                 


Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His 
225                 230                 235                 240 


Pro Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly 
                245                 250                 255     


Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly 
            260                 265                 270         


Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr 
        275                 280                 285             


Lys Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His 
    290                 295                 300                 


His His His His His 
305                 


<210>  148
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with E171G plus KRKKRKKRK peptide plus HIS-tag

<400>  148

Met Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn 
1               5                   10                  15      


Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr 
            20                  25                  30          


Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu 
        35                  40                  45              


Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly 
    50                  55                  60                  


Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr 
65                  70                  75                  80  


Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val 
                85                  90                  95      


Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe 
            100                 105                 110         


Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp 
        115                 120                 125             


Ser Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys 
    130                 135                 140                 


Gly Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys 
145                 150                 155                 160 


Met Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala 
                165                 170                 175     


Ala Gly Ser Gly Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala 
            180                 185                 190         


Asn Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu 
        195                 200                 205             


Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg 
    210                 215                 220                 


Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His 
225                 230                 235                 240 


Pro Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly 
                245                 250                 255     


Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly 
            260                 265                 270         


Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr 
        275                 280                 285             


Lys Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His 
    290                 295                 300                 


His His His His His 
305                 


<210>  149
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with Y173T plus KRKKRKKRK peptide plus HIS-tag

<400>  149

Met Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn 
1               5                   10                  15      


Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr 
            20                  25                  30          


Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu 
        35                  40                  45              


Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly 
    50                  55                  60                  


Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr 
65                  70                  75                  80  


Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val 
                85                  90                  95      


Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe 
            100                 105                 110         


Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp 
        115                 120                 125             


Ser Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys 
    130                 135                 140                 


Gly Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys 
145                 150                 155                 160 


Met Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala 
                165                 170                 175     


Ala Gly Ser Glu Ala Thr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala 
            180                 185                 190         


Asn Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu 
        195                 200                 205             


Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg 
    210                 215                 220                 


Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His 
225                 230                 235                 240 


Pro Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly 
                245                 250                 255     


Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly 
            260                 265                 270         


Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr 
        275                 280                 285             


Lys Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His 
    290                 295                 300                 


His His His His His 
305                 


<210>  150
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with A182T plus KRKKRKKRK peptide plus HIS-tag

<400>  150

Met Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn 
1               5                   10                  15      


Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr 
            20                  25                  30          


Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu 
        35                  40                  45              


Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly 
    50                  55                  60                  


Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr 
65                  70                  75                  80  


Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val 
                85                  90                  95      


Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe 
            100                 105                 110         


Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp 
        115                 120                 125             


Ser Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys 
    130                 135                 140                 


Gly Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys 
145                 150                 155                 160 


Met Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala 
                165                 170                 175     


Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Thr Ala 
            180                 185                 190         


Asn Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu 
        195                 200                 205             


Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg 
    210                 215                 220                 


Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His 
225                 230                 235                 240 


Pro Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly 
                245                 250                 255     


Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly 
            260                 265                 270         


Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr 
        275                 280                 285             


Lys Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His 
    290                 295                 300                 


His His His His His 
305                 


<210>  151
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with N187G plus KRKKRKKRK peptide plus HIS-tag

<400>  151

Met Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn 
1               5                   10                  15      


Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr 
            20                  25                  30          


Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu 
        35                  40                  45              


Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly 
    50                  55                  60                  


Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr 
65                  70                  75                  80  


Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val 
                85                  90                  95      


Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe 
            100                 105                 110         


Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp 
        115                 120                 125             


Ser Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys 
    130                 135                 140                 


Gly Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys 
145                 150                 155                 160 


Met Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala 
                165                 170                 175     


Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala 
            180                 185                 190         


Asn Asp Leu Gly Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu 
        195                 200                 205             


Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg 
    210                 215                 220                 


Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His 
225                 230                 235                 240 


Pro Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly 
                245                 250                 255     


Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly 
            260                 265                 270         


Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr 
        275                 280                 285             


Lys Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His 
    290                 295                 300                 


His His His His His 
305                 


<210>  152
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with D197S and Q198A plus KRKKRKKRK peptide plus 
       HIS-tag

<400>  152

Met Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn 
1               5                   10                  15      


Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr 
            20                  25                  30          


Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu 
        35                  40                  45              


Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly 
    50                  55                  60                  


Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr 
65                  70                  75                  80  


Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val 
                85                  90                  95      


Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe 
            100                 105                 110         


Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp 
        115                 120                 125             


Ser Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys 
    130                 135                 140                 


Gly Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys 
145                 150                 155                 160 


Met Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala 
                165                 170                 175     


Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala 
            180                 185                 190         


Asn Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Ser Ala Leu 
        195                 200                 205             


Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg 
    210                 215                 220                 


Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His 
225                 230                 235                 240 


Pro Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly 
                245                 250                 255     


Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly 
            260                 265                 270         


Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr 
        275                 280                 285             


Lys Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His 
    290                 295                 300                 


His His His His His 
305                 


<210>  153
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with L204Y plus KRKKRKKRK peptide plus HIS-tag

<400>  153

Met Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn 
1               5                   10                  15      


Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr 
            20                  25                  30          


Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu 
        35                  40                  45              


Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly 
    50                  55                  60                  


Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr 
65                  70                  75                  80  


Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val 
                85                  90                  95      


Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe 
            100                 105                 110         


Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp 
        115                 120                 125             


Ser Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys 
    130                 135                 140                 


Gly Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys 
145                 150                 155                 160 


Met Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala 
                165                 170                 175     


Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala 
            180                 185                 190         


Asn Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu 
        195                 200                 205             


Thr Gln Leu Asp Tyr Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg 
    210                 215                 220                 


Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His 
225                 230                 235                 240 


Pro Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly 
                245                 250                 255     


Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly 
            260                 265                 270         


Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr 
        275                 280                 285             


Lys Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His 
    290                 295                 300                 


His His His His His 
305                 


<210>  154
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with F230L plus KRKKRKKRK peptide plus HIS-tag

<400>  154

Met Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn 
1               5                   10                  15      


Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr 
            20                  25                  30          


Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu 
        35                  40                  45              


Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly 
    50                  55                  60                  


Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr 
65                  70                  75                  80  


Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val 
                85                  90                  95      


Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe 
            100                 105                 110         


Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp 
        115                 120                 125             


Ser Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys 
    130                 135                 140                 


Gly Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys 
145                 150                 155                 160 


Met Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala 
                165                 170                 175     


Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala 
            180                 185                 190         


Asn Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu 
        195                 200                 205             


Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg 
    210                 215                 220                 


Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Leu His 
225                 230                 235                 240 


Pro Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly 
                245                 250                 255     


Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly 
            260                 265                 270         


Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr 
        275                 280                 285             


Lys Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His 
    290                 295                 300                 


His His His His His 
305                 


<210>  155
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with H231Y plus KRKKRKKRK peptide plus HIS-tag

<400>  155

Met Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn 
1               5                   10                  15      


Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr 
            20                  25                  30          


Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu 
        35                  40                  45              


Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly 
    50                  55                  60                  


Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr 
65                  70                  75                  80  


Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val 
                85                  90                  95      


Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe 
            100                 105                 110         


Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp 
        115                 120                 125             


Ser Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys 
    130                 135                 140                 


Gly Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys 
145                 150                 155                 160 


Met Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala 
                165                 170                 175     


Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala 
            180                 185                 190         


Asn Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu 
        195                 200                 205             


Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg 
    210                 215                 220                 


Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe Tyr 
225                 230                 235                 240 


Pro Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly 
                245                 250                 255     


Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly 
            260                 265                 270         


Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr 
        275                 280                 285             


Lys Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His 
    290                 295                 300                 


His His His His His 
305                 


<210>  156
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with S234A plus KRKKRKKRK peptide plus HIS-tag

<400>  156

Met Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn 
1               5                   10                  15      


Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr 
            20                  25                  30          


Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu 
        35                  40                  45              


Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly 
    50                  55                  60                  


Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr 
65                  70                  75                  80  


Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val 
                85                  90                  95      


Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe 
            100                 105                 110         


Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp 
        115                 120                 125             


Ser Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys 
    130                 135                 140                 


Gly Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys 
145                 150                 155                 160 


Met Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala 
                165                 170                 175     


Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala 
            180                 185                 190         


Asn Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu 
        195                 200                 205             


Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg 
    210                 215                 220                 


Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His 
225                 230                 235                 240 


Pro Ala Ala Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly 
                245                 250                 255     


Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly 
            260                 265                 270         


Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr 
        275                 280                 285             


Lys Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His 
    290                 295                 300                 


His His His His His 
305                 


<210>  157
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with C135S plus KRKKRKKRK peptide plus HIS-tag

<400>  157

Met Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn 
1               5                   10                  15      


Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr 
            20                  25                  30          


Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu 
        35                  40                  45              


Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly 
    50                  55                  60                  


Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr 
65                  70                  75                  80  


Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val 
                85                  90                  95      


Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe 
            100                 105                 110         


Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp 
        115                 120                 125             


Ser Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Ser 
    130                 135                 140                 


Gly Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys 
145                 150                 155                 160 


Met Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala 
                165                 170                 175     


Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala 
            180                 185                 190         


Asn Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu 
        195                 200                 205             


Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg 
    210                 215                 220                 


Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His 
225                 230                 235                 240 


Pro Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly 
                245                 250                 255     


Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly 
            260                 265                 270         


Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr 
        275                 280                 285             


Lys Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His 
    290                 295                 300                 


His His His His His 
305                 


<210>  158
<211>  332
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with A182T plus SMAP peptide plus HIS-tag

<400>  158

Met Arg Gly Leu Arg Arg Leu Gly Arg Lys Ile Ala His Gly Val Lys 
1               5                   10                  15      


Lys Tyr Gly Pro Thr Val Leu Arg Ile Ile Arg Ile Ala Gly Gly Ser 
            20                  25                  30          


Met Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val 
        35                  40                  45              


Lys Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys 
    50                  55                  60                  


Gly Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val 
65                  70                  75                  80  


Val Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala 
                85                  90                  95      


Ser Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu 
            100                 105                 110         


Gly Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu 
        115                 120                 125             


Ser Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr 
    130                 135                 140                 


Pro Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr 
145                 150                 155                 160 


Ala Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala 
                165                 170                 175     


Pro His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe 
            180                 185                 190         


Ser Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile 
        195                 200                 205             


Gln Phe Met Ser Pro Thr Ala Asn Asp Leu Asn Val Pro Leu Ser Val 
    210                 215                 220                 


Ile Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr 
225                 230                 235                 240 


Phe Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp 
                245                 250                 255     


Phe Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp 
            260                 265                 270         


Glu Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly 
        275                 280                 285             


Phe Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser 
    290                 295                 300                 


Thr Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His 
305                 310                 315                 320 


Val Ile Ser Tyr Leu Glu His His His His His His 
                325                 330         


<210>  159
<211>  332
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with N187G plus SMAP peptide plus HIS-tag

<400>  159

Met Arg Gly Leu Arg Arg Leu Gly Arg Lys Ile Ala His Gly Val Lys 
1               5                   10                  15      


Lys Tyr Gly Pro Thr Val Leu Arg Ile Ile Arg Ile Ala Gly Gly Ser 
            20                  25                  30          


Met Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val 
        35                  40                  45              


Lys Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys 
    50                  55                  60                  


Gly Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val 
65                  70                  75                  80  


Val Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala 
                85                  90                  95      


Ser Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu 
            100                 105                 110         


Gly Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu 
        115                 120                 125             


Ser Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr 
    130                 135                 140                 


Pro Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr 
145                 150                 155                 160 


Ala Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala 
                165                 170                 175     


Pro His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe 
            180                 185                 190         


Ser Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile 
        195                 200                 205             


Gln Phe Met Ser Pro Ala Ala Asn Asp Leu Gly Val Pro Leu Ser Val 
    210                 215                 220                 


Ile Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr 
225                 230                 235                 240 


Phe Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp 
                245                 250                 255     


Phe Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp 
            260                 265                 270         


Glu Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly 
        275                 280                 285             


Phe Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser 
    290                 295                 300                 


Thr Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His 
305                 310                 315                 320 


Val Ile Ser Tyr Leu Glu His His His His His His 
                325                 330         


<210>  160
<211>  332
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with N187G and F230L plus SMAP peptide plus HIS-tag

<400>  160

Met Arg Gly Leu Arg Arg Leu Gly Arg Lys Ile Ala His Gly Val Lys 
1               5                   10                  15      


Lys Tyr Gly Pro Thr Val Leu Arg Ile Ile Arg Ile Ala Gly Gly Ser 
            20                  25                  30          


Met Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val 
        35                  40                  45              


Lys Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys 
    50                  55                  60                  


Gly Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val 
65                  70                  75                  80  


Val Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala 
                85                  90                  95      


Ser Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu 
            100                 105                 110         


Gly Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu 
        115                 120                 125             


Ser Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr 
    130                 135                 140                 


Pro Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr 
145                 150                 155                 160 


Ala Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala 
                165                 170                 175     


Pro His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe 
            180                 185                 190         


Ser Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile 
        195                 200                 205             


Gln Phe Met Ser Pro Ala Ala Asn Asp Leu Gly Val Pro Leu Ser Val 
    210                 215                 220                 


Ile Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr 
225                 230                 235                 240 


Phe Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp 
                245                 250                 255     


Phe Tyr Leu Thr Ile Leu His Pro Ala Ser Val Gly Lys Lys Ala Asp 
            260                 265                 270         


Glu Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly 
        275                 280                 285             


Phe Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser 
    290                 295                 300                 


Thr Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His 
305                 310                 315                 320 


Val Ile Ser Tyr Leu Glu His His His His His His 
                325                 330         


<210>  161
<211>  332
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with A182T, N187G and F230L plus SMAP peptide plus 
       HIS-tag

<400>  161

Met Arg Gly Leu Arg Arg Leu Gly Arg Lys Ile Ala His Gly Val Lys 
1               5                   10                  15      


Lys Tyr Gly Pro Thr Val Leu Arg Ile Ile Arg Ile Ala Gly Gly Ser 
            20                  25                  30          


Met Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val 
        35                  40                  45              


Lys Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys 
    50                  55                  60                  


Gly Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val 
65                  70                  75                  80  


Val Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala 
                85                  90                  95      


Ser Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu 
            100                 105                 110         


Gly Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu 
        115                 120                 125             


Ser Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr 
    130                 135                 140                 


Pro Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr 
145                 150                 155                 160 


Ala Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala 
                165                 170                 175     


Pro His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe 
            180                 185                 190         


Ser Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile 
        195                 200                 205             


Gln Phe Met Ser Pro Thr Ala Asn Asp Leu Gly Val Pro Leu Ser Val 
    210                 215                 220                 


Ile Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr 
225                 230                 235                 240 


Phe Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp 
                245                 250                 255     


Phe Tyr Leu Thr Ile Leu His Pro Ala Ser Val Gly Lys Lys Ala Asp 
            260                 265                 270         


Glu Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly 
        275                 280                 285             


Phe Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser 
    290                 295                 300                 


Thr Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His 
305                 310                 315                 320 


Val Ile Ser Tyr Leu Glu His His His His His His 
                325                 330         


<210>  162
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with S120G plus KRKKRKKRK peptide plus HIS-tag 
       without N-terminal methionine

<400>  162

Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn Gly 
1               5                   10                  15      


Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr Gly 
            20                  25                  30          


Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu Thr 
        35                  40                  45              


Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly Ile 
    50                  55                  60                  


Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr Ala 
65                  70                  75                  80  


Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val Asp 
                85                  90                  95      


Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe Glu 
            100                 105                 110         


Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp Gly 
        115                 120                 125             


Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys Gly 
    130                 135                 140                 


Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys Met 
145                 150                 155                 160 


Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala Ala 
                165                 170                 175     


Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala Asn 
            180                 185                 190         


Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu Thr 
        195                 200                 205             


Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg Gly 
    210                 215                 220                 


Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His Pro 
225                 230                 235                 240 


Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly Ser 
                245                 250                 255     


Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly Lys 
            260                 265                 270         


Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr Lys 
        275                 280                 285             


Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His His 
    290                 295                 300                 


His His His His 
305             


<210>  163
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with A126E and T128K plus KRKKRKKRK peptide plus 
       HIS-tag without N-terminal methionine

<400>  163

Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn Gly 
1               5                   10                  15      


Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr Gly 
            20                  25                  30          


Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu Thr 
        35                  40                  45              


Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly Ile 
    50                  55                  60                  


Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr Ala 
65                  70                  75                  80  


Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val Asp 
                85                  90                  95      


Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe Glu 
            100                 105                 110         


Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp Ser 
        115                 120                 125             


Gly Lys Val Ser Pro Glu Phe Lys Ala Lys Val Lys Asp Trp Cys Gly 
    130                 135                 140                 


Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys Met 
145                 150                 155                 160 


Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala Ala 
                165                 170                 175     


Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala Asn 
            180                 185                 190         


Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu Thr 
        195                 200                 205             


Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg Gly 
    210                 215                 220                 


Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His Pro 
225                 230                 235                 240 


Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly Ser 
                245                 250                 255     


Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly Lys 
            260                 265                 270         


Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr Lys 
        275                 280                 285             


Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His His 
    290                 295                 300                 


His His His His 
305             


<210>  164
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with W134I plus KRKKRKKRK peptide plus HIS-tag 
       without N-terminal methionine

<400>  164

Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn Gly 
1               5                   10                  15      


Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr Gly 
            20                  25                  30          


Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu Thr 
        35                  40                  45              


Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly Ile 
    50                  55                  60                  


Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr Ala 
65                  70                  75                  80  


Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val Asp 
                85                  90                  95      


Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe Glu 
            100                 105                 110         


Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp Ser 
        115                 120                 125             


Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Ile Cys Gly 
    130                 135                 140                 


Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys Met 
145                 150                 155                 160 


Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala Ala 
                165                 170                 175     


Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala Asn 
            180                 185                 190         


Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu Thr 
        195                 200                 205             


Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg Gly 
    210                 215                 220                 


Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His Pro 
225                 230                 235                 240 


Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly Ser 
                245                 250                 255     


Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly Lys 
            260                 265                 270         


Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr Lys 
        275                 280                 285             


Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His His 
    290                 295                 300                 


His His His His 
305             


<210>  165
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with H138L plus KRKKRKKRK peptide plus HIS-tag 
       without N-terminal methionine

<400>  165

Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn Gly 
1               5                   10                  15      


Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr Gly 
            20                  25                  30          


Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu Thr 
        35                  40                  45              


Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly Ile 
    50                  55                  60                  


Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr Ala 
65                  70                  75                  80  


Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val Asp 
                85                  90                  95      


Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe Glu 
            100                 105                 110         


Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp Ser 
        115                 120                 125             


Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys Gly 
    130                 135                 140                 


Val Leu Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys Met 
145                 150                 155                 160 


Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala Ala 
                165                 170                 175     


Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala Asn 
            180                 185                 190         


Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu Thr 
        195                 200                 205             


Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg Gly 
    210                 215                 220                 


Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His Pro 
225                 230                 235                 240 


Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly Ser 
                245                 250                 255     


Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly Lys 
            260                 265                 270         


Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr Lys 
        275                 280                 285             


Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His His 
    290                 295                 300                 


His His His His 
305             


<210>  166
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with E171G plus KRKKRKKRK peptide plus HIS-tag 
       without N-terminal methionine

<400>  166

Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn Gly 
1               5                   10                  15      


Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr Gly 
            20                  25                  30          


Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu Thr 
        35                  40                  45              


Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly Ile 
    50                  55                  60                  


Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr Ala 
65                  70                  75                  80  


Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val Asp 
                85                  90                  95      


Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe Glu 
            100                 105                 110         


Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp Ser 
        115                 120                 125             


Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys Gly 
    130                 135                 140                 


Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys Met 
145                 150                 155                 160 


Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala Ala 
                165                 170                 175     


Gly Ser Gly Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala Asn 
            180                 185                 190         


Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu Thr 
        195                 200                 205             


Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg Gly 
    210                 215                 220                 


Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His Pro 
225                 230                 235                 240 


Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly Ser 
                245                 250                 255     


Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly Lys 
            260                 265                 270         


Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr Lys 
        275                 280                 285             


Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His His 
    290                 295                 300                 


His His His His 
305             


<210>  167
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with Y173T plus KRKKRKKRK peptide plus HIS-tag 
       without N-terminal methionine

<400>  167

Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn Gly 
1               5                   10                  15      


Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr Gly 
            20                  25                  30          


Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu Thr 
        35                  40                  45              


Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly Ile 
    50                  55                  60                  


Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr Ala 
65                  70                  75                  80  


Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val Asp 
                85                  90                  95      


Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe Glu 
            100                 105                 110         


Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp Ser 
        115                 120                 125             


Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys Gly 
    130                 135                 140                 


Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys Met 
145                 150                 155                 160 


Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala Ala 
                165                 170                 175     


Gly Ser Glu Ala Thr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala Asn 
            180                 185                 190         


Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu Thr 
        195                 200                 205             


Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg Gly 
    210                 215                 220                 


Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His Pro 
225                 230                 235                 240 


Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly Ser 
                245                 250                 255     


Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly Lys 
            260                 265                 270         


Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr Lys 
        275                 280                 285             


Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His His 
    290                 295                 300                 


His His His His 
305             


<210>  168
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with A182T plus KRKKRKKRK peptide plus HIS-tag 
       without N-terminal methionine

<400>  168

Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn Gly 
1               5                   10                  15      


Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr Gly 
            20                  25                  30          


Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu Thr 
        35                  40                  45              


Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly Ile 
    50                  55                  60                  


Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr Ala 
65                  70                  75                  80  


Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val Asp 
                85                  90                  95      


Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe Glu 
            100                 105                 110         


Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp Ser 
        115                 120                 125             


Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys Gly 
    130                 135                 140                 


Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys Met 
145                 150                 155                 160 


Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala Ala 
                165                 170                 175     


Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Thr Ala Asn 
            180                 185                 190         


Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu Thr 
        195                 200                 205             


Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg Gly 
    210                 215                 220                 


Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His Pro 
225                 230                 235                 240 


Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly Ser 
                245                 250                 255     


Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly Lys 
            260                 265                 270         


Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr Lys 
        275                 280                 285             


Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His His 
    290                 295                 300                 


His His His His 
305             


<210>  169
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with N187G plus KRKKRKKRK peptide plus HIS-tag 
       without N-terminal methionine

<400>  169

Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn Gly 
1               5                   10                  15      


Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr Gly 
            20                  25                  30          


Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu Thr 
        35                  40                  45              


Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly Ile 
    50                  55                  60                  


Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr Ala 
65                  70                  75                  80  


Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val Asp 
                85                  90                  95      


Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe Glu 
            100                 105                 110         


Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp Ser 
        115                 120                 125             


Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys Gly 
    130                 135                 140                 


Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys Met 
145                 150                 155                 160 


Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala Ala 
                165                 170                 175     


Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala Asn 
            180                 185                 190         


Asp Leu Gly Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu Thr 
        195                 200                 205             


Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg Gly 
    210                 215                 220                 


Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His Pro 
225                 230                 235                 240 


Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly Ser 
                245                 250                 255     


Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly Lys 
            260                 265                 270         


Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr Lys 
        275                 280                 285             


Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His His 
    290                 295                 300                 


His His His His 
305             


<210>  170
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with D197S and Q198A plus KRKKRKKRK peptide plus 
       HIS-tag without N-terminal methionine

<400>  170

Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn Gly 
1               5                   10                  15      


Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr Gly 
            20                  25                  30          


Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu Thr 
        35                  40                  45              


Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly Ile 
    50                  55                  60                  


Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr Ala 
65                  70                  75                  80  


Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val Asp 
                85                  90                  95      


Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe Glu 
            100                 105                 110         


Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp Ser 
        115                 120                 125             


Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys Gly 
    130                 135                 140                 


Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys Met 
145                 150                 155                 160 


Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala Ala 
                165                 170                 175     


Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala Asn 
            180                 185                 190         


Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Ser Ala Leu Thr 
        195                 200                 205             


Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg Gly 
    210                 215                 220                 


Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His Pro 
225                 230                 235                 240 


Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly Ser 
                245                 250                 255     


Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly Lys 
            260                 265                 270         


Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr Lys 
        275                 280                 285             


Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His His 
    290                 295                 300                 


His His His His 
305             


<210>  171
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with L204Y plus KRKKRKKRK peptide plus HIS-tag 
       without N-terminal methionine

<400>  171

Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn Gly 
1               5                   10                  15      


Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr Gly 
            20                  25                  30          


Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu Thr 
        35                  40                  45              


Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly Ile 
    50                  55                  60                  


Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr Ala 
65                  70                  75                  80  


Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val Asp 
                85                  90                  95      


Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe Glu 
            100                 105                 110         


Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp Ser 
        115                 120                 125             


Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys Gly 
    130                 135                 140                 


Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys Met 
145                 150                 155                 160 


Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala Ala 
                165                 170                 175     


Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala Asn 
            180                 185                 190         


Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu Thr 
        195                 200                 205             


Gln Leu Asp Tyr Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg Gly 
    210                 215                 220                 


Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His Pro 
225                 230                 235                 240 


Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly Ser 
                245                 250                 255     


Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly Lys 
            260                 265                 270         


Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr Lys 
        275                 280                 285             


Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His His 
    290                 295                 300                 


His His His His 
305             


<210>  172
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with F230L plus KRKKRKKRK peptide plus HIS-tag 
       without N-terminal methionine

<400>  172

Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn Gly 
1               5                   10                  15      


Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr Gly 
            20                  25                  30          


Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu Thr 
        35                  40                  45              


Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly Ile 
    50                  55                  60                  


Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr Ala 
65                  70                  75                  80  


Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val Asp 
                85                  90                  95      


Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe Glu 
            100                 105                 110         


Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp Ser 
        115                 120                 125             


Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys Gly 
    130                 135                 140                 


Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys Met 
145                 150                 155                 160 


Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala Ala 
                165                 170                 175     


Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala Asn 
            180                 185                 190         


Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu Thr 
        195                 200                 205             


Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg Gly 
    210                 215                 220                 


Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Leu His Pro 
225                 230                 235                 240 


Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly Ser 
                245                 250                 255     


Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly Lys 
            260                 265                 270         


Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr Lys 
        275                 280                 285             


Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His His 
    290                 295                 300                 


His His His His 
305             


<210>  173
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with H231Y plus KRKKRKKRK peptide plus HIS-tag 
       without N-terminal methionine

<400>  173

Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn Gly 
1               5                   10                  15      


Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr Gly 
            20                  25                  30          


Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu Thr 
        35                  40                  45              


Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly Ile 
    50                  55                  60                  


Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr Ala 
65                  70                  75                  80  


Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val Asp 
                85                  90                  95      


Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe Glu 
            100                 105                 110         


Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp Ser 
        115                 120                 125             


Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys Gly 
    130                 135                 140                 


Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys Met 
145                 150                 155                 160 


Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala Ala 
                165                 170                 175     


Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala Asn 
            180                 185                 190         


Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu Thr 
        195                 200                 205             


Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg Gly 
    210                 215                 220                 


Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe Tyr Pro 
225                 230                 235                 240 


Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly Ser 
                245                 250                 255     


Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly Lys 
            260                 265                 270         


Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr Lys 
        275                 280                 285             


Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His His 
    290                 295                 300                 


His His His His 
305             


<210>  174
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with S234A plus KRKKRKKRK peptide plus HIS-tag 
       without N-terminal methionine

<400>  174

Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn Gly 
1               5                   10                  15      


Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr Gly 
            20                  25                  30          


Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu Thr 
        35                  40                  45              


Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly Ile 
    50                  55                  60                  


Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr Ala 
65                  70                  75                  80  


Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val Asp 
                85                  90                  95      


Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe Glu 
            100                 105                 110         


Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp Ser 
        115                 120                 125             


Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys Gly 
    130                 135                 140                 


Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys Met 
145                 150                 155                 160 


Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala Ala 
                165                 170                 175     


Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala Asn 
            180                 185                 190         


Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu Thr 
        195                 200                 205             


Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg Gly 
    210                 215                 220                 


Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His Pro 
225                 230                 235                 240 


Ala Ala Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly Ser 
                245                 250                 255     


Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly Lys 
            260                 265                 270         


Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr Lys 
        275                 280                 285             


Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His His 
    290                 295                 300                 


His His His His 
305             


<210>  175
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with C135S plus KRKKRKKRK peptide plus HIS-tag 
       without N-terminal methionine

<400>  175

Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn Gly 
1               5                   10                  15      


Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr Gly 
            20                  25                  30          


Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu Thr 
        35                  40                  45              


Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly Ile 
    50                  55                  60                  


Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr Ala 
65                  70                  75                  80  


Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val Asp 
                85                  90                  95      


Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe Glu 
            100                 105                 110         


Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp Ser 
        115                 120                 125             


Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Ser Gly 
    130                 135                 140                 


Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys Met 
145                 150                 155                 160 


Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala Ala 
                165                 170                 175     


Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala Asn 
            180                 185                 190         


Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu Thr 
        195                 200                 205             


Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg Gly 
    210                 215                 220                 


Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His Pro 
225                 230                 235                 240 


Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly Ser 
                245                 250                 255     


Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly Lys 
            260                 265                 270         


Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr Lys 
        275                 280                 285             


Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His His 
    290                 295                 300                 


His His His His 
305             


<210>  176
<211>  331
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with A182T plus SMAP peptide plus HIS-tag without 
       N-terminal methionine

<400>  176

Arg Gly Leu Arg Arg Leu Gly Arg Lys Ile Ala His Gly Val Lys Lys 
1               5                   10                  15      


Tyr Gly Pro Thr Val Leu Arg Ile Ile Arg Ile Ala Gly Gly Ser Met 
            20                  25                  30          


Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys 
        35                  40                  45              


Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly 
    50                  55                  60                  


Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val Val 
65                  70                  75                  80  


Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser 
                85                  90                  95      


Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly 
            100                 105                 110         


Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser 
        115                 120                 125             


Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro 
    130                 135                 140                 


Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr Ala 
145                 150                 155                 160 


Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala Pro 
                165                 170                 175     


His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe Ser 
            180                 185                 190         


Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln 
        195                 200                 205             


Phe Met Ser Pro Thr Ala Asn Asp Leu Asn Val Pro Leu Ser Val Ile 
    210                 215                 220                 


Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe 
225                 230                 235                 240 


Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe 
                245                 250                 255     


Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp Glu 
            260                 265                 270         


Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe 
        275                 280                 285             


Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr 
    290                 295                 300                 


Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His Val 
305                 310                 315                 320 


Ile Ser Tyr Leu Glu His His His His His His 
                325                 330     


<210>  177
<211>  331
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with N187G plus SMAP peptide plus HIS-tag without 
       N-terminal methionine

<400>  177

Arg Gly Leu Arg Arg Leu Gly Arg Lys Ile Ala His Gly Val Lys Lys 
1               5                   10                  15      


Tyr Gly Pro Thr Val Leu Arg Ile Ile Arg Ile Ala Gly Gly Ser Met 
            20                  25                  30          


Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys 
        35                  40                  45              


Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly 
    50                  55                  60                  


Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val Val 
65                  70                  75                  80  


Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser 
                85                  90                  95      


Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly 
            100                 105                 110         


Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser 
        115                 120                 125             


Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro 
    130                 135                 140                 


Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr Ala 
145                 150                 155                 160 


Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala Pro 
                165                 170                 175     


His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe Ser 
            180                 185                 190         


Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln 
        195                 200                 205             


Phe Met Ser Pro Ala Ala Asn Asp Leu Gly Val Pro Leu Ser Val Ile 
    210                 215                 220                 


Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe 
225                 230                 235                 240 


Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe 
                245                 250                 255     


Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp Glu 
            260                 265                 270         


Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe 
        275                 280                 285             


Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr 
    290                 295                 300                 


Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His Val 
305                 310                 315                 320 


Ile Ser Tyr Leu Glu His His His His His His 
                325                 330     


<210>  178
<211>  331
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with N187G and F230L plus SMAP peptide plus HIS-tag
       without N-terminal methionine

<400>  178

Arg Gly Leu Arg Arg Leu Gly Arg Lys Ile Ala His Gly Val Lys Lys 
1               5                   10                  15      


Tyr Gly Pro Thr Val Leu Arg Ile Ile Arg Ile Ala Gly Gly Ser Met 
            20                  25                  30          


Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys 
        35                  40                  45              


Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly 
    50                  55                  60                  


Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val Val 
65                  70                  75                  80  


Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser 
                85                  90                  95      


Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly 
            100                 105                 110         


Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser 
        115                 120                 125             


Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro 
    130                 135                 140                 


Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr Ala 
145                 150                 155                 160 


Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala Pro 
                165                 170                 175     


His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe Ser 
            180                 185                 190         


Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln 
        195                 200                 205             


Phe Met Ser Pro Ala Ala Asn Asp Leu Gly Val Pro Leu Ser Val Ile 
    210                 215                 220                 


Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe 
225                 230                 235                 240 


Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe 
                245                 250                 255     


Tyr Leu Thr Ile Leu His Pro Ala Ser Val Gly Lys Lys Ala Asp Glu 
            260                 265                 270         


Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe 
        275                 280                 285             


Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr 
    290                 295                 300                 


Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His Val 
305                 310                 315                 320 


Ile Ser Tyr Leu Glu His His His His His His 
                325                 330     


<210>  179
<211>  331
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated EL188 with A182T, N187G and F230L plus SMAP peptide plus 
       HIS-tag without N-terminal methionine

<400>  179

Arg Gly Leu Arg Arg Leu Gly Arg Lys Ile Ala His Gly Val Lys Lys 
1               5                   10                  15      


Tyr Gly Pro Thr Val Leu Arg Ile Ile Arg Ile Ala Gly Gly Ser Met 
            20                  25                  30          


Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys 
        35                  40                  45              


Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly 
    50                  55                  60                  


Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val Val 
65                  70                  75                  80  


Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser 
                85                  90                  95      


Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly 
            100                 105                 110         


Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser 
        115                 120                 125             


Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro 
    130                 135                 140                 


Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr Ala 
145                 150                 155                 160 


Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala Pro 
                165                 170                 175     


His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe Ser 
            180                 185                 190         


Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln 
        195                 200                 205             


Phe Met Ser Pro Thr Ala Asn Asp Leu Gly Val Pro Leu Ser Val Ile 
    210                 215                 220                 


Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe 
225                 230                 235                 240 


Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe 
                245                 250                 255     


Tyr Leu Thr Ile Leu His Pro Ala Ser Val Gly Lys Lys Ala Asp Glu 
            260                 265                 270         


Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe 
        275                 280                 285             


Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr 
    290                 295                 300                 


Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His Val 
305                 310                 315                 320 


Ile Ser Tyr Leu Glu His His His His His His 
                325                 330     


<210>  180
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EL188 plus KRKKRKKRK peptide plus HIS-tag

<400>  180

Met Lys Arg Lys Lys Arg Lys Lys Arg Lys Asn Phe Arg Thr Lys Asn 
1               5                   10                  15      


Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr 
            20                  25                  30          


Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu 
        35                  40                  45              


Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly 
    50                  55                  60                  


Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr 
65                  70                  75                  80  


Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val 
                85                  90                  95      


Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe 
            100                 105                 110         


Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp 
        115                 120                 125             


Ser Gly Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys 
    130                 135                 140                 


Gly Val His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys 
145                 150                 155                 160 


Met Ala Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala 
                165                 170                 175     


Ala Gly Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala 
            180                 185                 190         


Asn Asp Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu 
        195                 200                 205             


Thr Gln Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg 
    210                 215                 220                 


Gly Lys Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His 
225                 230                 235                 240 


Pro Ala Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly 
                245                 250                 255     


Ser Lys Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly 
            260                 265                 270         


Lys Ile Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr 
        275                 280                 285             


Lys Gly Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His 
    290                 295                 300                 


His His His His His 
305                 


<210>  181
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EL188 plus SMAP peptide plus HIS-tag without linker

<400>  181

Met Arg Gly Leu Arg Arg Leu Gly Arg Lys Ile Ala His Gly Val Lys 
1               5                   10                  15      


Lys Tyr Gly Pro Thr Val Leu Arg Ile Ile Arg Ile Ala Gly Gly Ser 
            20                  25                  30          


Met Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val 
        35                  40                  45              


Lys Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys 
    50                  55                  60                  


Gly Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val 
65                  70                  75                  80  


Val Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala 
                85                  90                  95      


Ser Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu 
            100                 105                 110         


Gly Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu 
        115                 120                 125             


Ser Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr 
    130                 135                 140                 


Pro Thr Tyr Asp Ile Ala Trp Ser Gly Lys Val Ser Pro Ala Phe Thr 
145                 150                 155                 160 


Ala Lys Val Lys Asp Trp Cys Gly Val His Val Pro Asn His Arg Ala 
                165                 170                 175     


Pro His Trp Leu Met Ala Cys Met Ala Phe Glu Thr Gly Gln Thr Phe 
            180                 185                 190         


Ser Pro Ser Ile Lys Asn Ala Ala Gly Ser Glu Ala Tyr Gly Leu Ile 
        195                 200                 205             


Gln Phe Met Ser Pro Ala Ala Asn Asp Leu Asn Val Pro Leu Ser Val 
    210                 215                 220                 


Ile Arg Ser Met Asp Gln Leu Thr Gln Leu Asp Leu Val Phe Lys Tyr 
225                 230                 235                 240 


Phe Glu Met Trp Met Lys Arg Gly Lys Arg Tyr Thr Gln Leu Glu Asp 
                245                 250                 255     


Phe Tyr Leu Thr Ile Phe His Pro Ala Ser Val Gly Lys Lys Ala Asp 
            260                 265                 270         


Glu Val Leu Phe Leu Gln Gly Ser Lys Ala Tyr Leu Gln Asn Lys Gly 
        275                 280                 285             


Phe Asp Val Asp Lys Asp Gly Lys Ile Thr Leu Gly Glu Ile Ser Ser 
    290                 295                 300                 


Thr Leu Tyr Thr Thr Tyr Tyr Lys Gly Leu Leu Pro Glu Asn Arg His 
305                 310                 315                 320 


Val Ile Ser Tyr Leu Glu 
                325     


<210>  182
<211>  339
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EL188 plus SMAP peptide plus HIS-tag with GAGAGAGA-linker

<400>  182

Met Arg Gly Leu Arg Arg Leu Gly Arg Lys Ile Ala His Gly Val Lys 
1               5                   10                  15      


Lys Tyr Gly Pro Thr Val Leu Arg Ile Ile Arg Ile Ala Gly Gly Ser 
            20                  25                  30          


Gly Ala Gly Ala Gly Ala Gly Ala Asn Phe Arg Thr Lys Asn Gly Tyr 
        35                  40                  45              


Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly Leu Tyr Thr Gly Gln 
    50                  55                  60                  


Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser Ser Thr Glu Thr Leu 
65                  70                  75                  80  


Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn Thr Gly Gly Ile Gly 
                85                  90                  95      


Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn Val Ile Thr Ala Leu 
            100                 105                 110         


Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser Leu Thr Val Asp Gly 
        115                 120                 125             


Ile Trp Gly Asn Gly Thr Leu Ser Gly Leu Asp Lys Ala Phe Glu Val 
    130                 135                 140                 


Tyr Lys Glu Arg Tyr Arg Thr Pro Thr Tyr Asp Ile Ala Trp Ser Gly 
145                 150                 155                 160 


Lys Val Ser Pro Ala Phe Thr Ala Lys Val Lys Asp Trp Cys Gly Val 
                165                 170                 175     


His Val Pro Asn His Arg Ala Pro His Trp Leu Met Ala Cys Met Ala 
            180                 185                 190         


Phe Glu Thr Gly Gln Thr Phe Ser Pro Ser Ile Lys Asn Ala Ala Gly 
        195                 200                 205             


Ser Glu Ala Tyr Gly Leu Ile Gln Phe Met Ser Pro Ala Ala Asn Asp 
    210                 215                 220                 


Leu Asn Val Pro Leu Ser Val Ile Arg Ser Met Asp Gln Leu Thr Gln 
225                 230                 235                 240 


Leu Asp Leu Val Phe Lys Tyr Phe Glu Met Trp Met Lys Arg Gly Lys 
                245                 250                 255     


Arg Tyr Thr Gln Leu Glu Asp Phe Tyr Leu Thr Ile Phe His Pro Ala 
            260                 265                 270         


Ser Val Gly Lys Lys Ala Asp Glu Val Leu Phe Leu Gln Gly Ser Lys 
        275                 280                 285             


Ala Tyr Leu Gln Asn Lys Gly Phe Asp Val Asp Lys Asp Gly Lys Ile 
    290                 295                 300                 


Thr Leu Gly Glu Ile Ser Ser Thr Leu Tyr Thr Thr Tyr Tyr Lys Gly 
305                 310                 315                 320 


Leu Leu Pro Glu Asn Arg His Val Ile Ser Tyr Leu Glu His His His 
                325                 330                 335     


His His His 
            


