                         SEQUENCE LISTING

<110>  Merial Limited
       Lawrence, Paulraj
       Bey, Russell
 
<120>  Attenuated Pasteurella Multocida Vaccines & Methods of Making & 
       Use Thereof.

<130>  MER 13-217

<150>  USSN 61/898,497
<151>  2013-11-01

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  2919
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pm 1062 hyaD nucleotide sequence

<400>  1
atgaatacat tatcacaagc aataaaagca tataacagca atgactatca attagcactc       60

aaattatttg aaaagtcggc ggaaatctat ggacggaaaa ttgttgaatt tcaaattacc      120

aaatgcaaag aaaaactctc agcacatcct tctgttaatt cagcacatct ttctgtaaat      180

aaagaagaaa aagtcaatgt ttgcgatagt ccgttagata ttgcaacaca actgttactt      240

tccaacgtaa aaaaattagt actttctgac tcggaaaaaa acacgttaaa aaataaatgg      300

aaattgctca ctgagaagaa atctgaaaat gcggaggtaa gagcggtcgc ccttgtacca      360

aaagattttc ccaaagatct ggttttagcg cctttacctg atcatgttaa tgattttaca      420

tggtacaaaa agcgaaagaa aagacttggc ataaaacctg aacatcaaca tgttggtctt      480

tctattatcg ttacaacatt caatcgacca gcaattttat cgattacatt agcctgttta      540

gtaaaccaaa aaacacatta cccgtttgaa gttatcgtga cagatgatgg tagtcaggaa      600

gatctatcac cgatcattcg ccaatatgaa aataaattgg atattcgcta cgtcagacaa      660

aaagataacg gttttcaagc cagtgccgct cggaatatgg gattacgctt agcaaaatat      720

gactttattg gcttactcga ctgtgatatg gcgccaaatc cattatgggt tcattcttat      780

gttgcagagc tattagaaga tgatgattta acaatcattg gtccaagaaa atacatcgat      840

acacaacata ttgacccaaa agacttctta aataacgcga gtttgcttga atcattacca      900

gaagtgaaaa ccaataatag tgttgccgca aaaggggaag gaacagtttc tctggattgg      960

cgcttagaac aattcgaaaa aacagaaaat ctccgcttat ccgattcgcc tttccgtttt     1020

tttgcggcgg gtaatgttgc tttcgctaaa aaatggctaa ataaatccgg tttctttgat     1080

gaggaattta atcactgggg tggagaagat gtggaatttg gatatcgctt attccgttac     1140

ggtagtttct ttaaaactat tgatggcatt atggcctacc atcaagagcc accaggtaaa     1200

gaaaatgaaa ccgatcgtga agcgggaaaa aatattacgc tcgatattat gagagaaaag     1260

gtcccttata tctatagaaa acttttacca atagaagatt cgcatatcaa tagagtacct     1320

ttagtttcaa tttatatccc agcttataac tgtgcaaact atattcaacg ttgcgtagat     1380

agtgcactga atcagactgt tgttgatctc gaggtttgta tttgtaacga tggttcaaca     1440

gataatacct tagaagtgat caataagctt tatggtaata atcctagggt acgcatcatg     1500

tctaaaccaa atggcggaat agcctcagca tcaaatgcag ccgtttcttt tgctaaaggt     1560

tattacattg ggcagttaga ttcagatgat tatcttgagc ctgatgcagt tgaactgtgt     1620

ttaaaagaat ttttaaaaga taaaacgcta gcttgtgttt ataccactaa tagaaacgtc     1680

aatccggatg gtagcttaat cgctaatggt tacaattggc cagaattttc acgagaaaaa     1740

ctcacaacgg ctatgattgc tcaccacttt agaatgttca cgattagagc ttggcattta     1800

actgatggat tcaatgaaaa aattgaaaat gccgtagact atgacatgtt cctcaaactc     1860

agtgaagttg gaaaatttaa acatcttaat aaaatctgct ataaccgtgt attacatggt     1920

gataacacat caattaagaa acttggcatt caaaagaaaa accattttgt tgtagtcaat     1980

cagtcattaa atagacaagg cataacttat tataattatg acgaatttga tgatttagat     2040

gaaagtagaa agtatatttt caataaaacc gctgaatatc aagaagagat tgatatctta     2100

aaagatatta aaatcatcca gaataaagat gccaaaatcg cagtcagtat tttttatccc     2160

aatacattaa acggcttagt gaaaaaacta aacaatatta ttgaatataa taaaaatata     2220

ttcgttattg ttctacatgt tgataagaat catcttacac cagatatcaa aaaagaaata     2280

ctagccttct atcataaaca tcaagtgaat attttactaa ataatgatat ctcatattac     2340

acgagtaata gattaataaa aactgaggcg catttaagta atattaataa attaagtcag     2400

ttaaatctaa attgtgaata catcattttt gataatcatg acagcctatt cgttaaaaat     2460

gacagctatg cttatatgaa aaaatatgat gtcggcatga atttctcagc attaacacat     2520

gattggatcg agaaaatcaa tgcgcatcca ccatttaaaa agctcattaa aacttatttt     2580

aatgacaatg acttaaaaag tatgaatgtg aaaggggcat cacaaggtat gtttatgacg     2640

tatgcgctag cgcatgagct tctgacgatt attaaagaag tcatcacatc ttgccagtca     2700

attgatagtg tgccagaata taacactgag gatatttggt tccaatttgc acttttaatc     2760

ttagaaaaga aaaccggcca tgtatttaat aaaacatcga ccctgactta tatgccttgg     2820

gaacgaaaat tacaatggac aaatgaacaa attgaaagtg caaaaagagg agaaaatata     2880

cctgttaaca agttcattat taatagtata actctataa                            2919


<210>  2
<211>  972
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pm 1062 hyaD amino acid sequence

<400>  2

Met Asn Thr Leu Ser Gln Ala Ile Lys Ala Tyr Asn Ser Asn Asp Tyr 
1               5                   10                  15      


Gln Leu Ala Leu Lys Leu Phe Glu Lys Ser Ala Glu Ile Tyr Gly Arg 
            20                  25                  30          


Lys Ile Val Glu Phe Gln Ile Thr Lys Cys Lys Glu Lys Leu Ser Ala 
        35                  40                  45              


His Pro Ser Val Asn Ser Ala His Leu Ser Val Asn Lys Glu Glu Lys 
    50                  55                  60                  


Val Asn Val Cys Asp Ser Pro Leu Asp Ile Ala Thr Gln Leu Leu Leu 
65                  70                  75                  80  


Ser Asn Val Lys Lys Leu Val Leu Ser Asp Ser Glu Lys Asn Thr Leu 
                85                  90                  95      


Lys Asn Lys Trp Lys Leu Leu Thr Glu Lys Lys Ser Glu Asn Ala Glu 
            100                 105                 110         


Val Arg Ala Val Ala Leu Val Pro Lys Asp Phe Pro Lys Asp Leu Val 
        115                 120                 125             


Leu Ala Pro Leu Pro Asp His Val Asn Asp Phe Thr Trp Tyr Lys Lys 
    130                 135                 140                 


Arg Lys Lys Arg Leu Gly Ile Lys Pro Glu His Gln His Val Gly Leu 
145                 150                 155                 160 


Ser Ile Ile Val Thr Thr Phe Asn Arg Pro Ala Ile Leu Ser Ile Thr 
                165                 170                 175     


Leu Ala Cys Leu Val Asn Gln Lys Thr His Tyr Pro Phe Glu Val Ile 
            180                 185                 190         


Val Thr Asp Asp Gly Ser Gln Glu Asp Leu Ser Pro Ile Ile Arg Gln 
        195                 200                 205             


Tyr Glu Asn Lys Leu Asp Ile Arg Tyr Val Arg Gln Lys Asp Asn Gly 
    210                 215                 220                 


Phe Gln Ala Ser Ala Ala Arg Asn Met Gly Leu Arg Leu Ala Lys Tyr 
225                 230                 235                 240 


Asp Phe Ile Gly Leu Leu Asp Cys Asp Met Ala Pro Asn Pro Leu Trp 
                245                 250                 255     


Val His Ser Tyr Val Ala Glu Leu Leu Glu Asp Asp Asp Leu Thr Ile 
            260                 265                 270         


Ile Gly Pro Arg Lys Tyr Ile Asp Thr Gln His Ile Asp Pro Lys Asp 
        275                 280                 285             


Phe Leu Asn Asn Ala Ser Leu Leu Glu Ser Leu Pro Glu Val Lys Thr 
    290                 295                 300                 


Asn Asn Ser Val Ala Ala Lys Gly Glu Gly Thr Val Ser Leu Asp Trp 
305                 310                 315                 320 


Arg Leu Glu Gln Phe Glu Lys Thr Glu Asn Leu Arg Leu Ser Asp Ser 
                325                 330                 335     


Pro Phe Arg Phe Phe Ala Ala Gly Asn Val Ala Phe Ala Lys Lys Trp 
            340                 345                 350         


Leu Asn Lys Ser Gly Phe Phe Asp Glu Glu Phe Asn His Trp Gly Gly 
        355                 360                 365             


Glu Asp Val Glu Phe Gly Tyr Arg Leu Phe Arg Tyr Gly Ser Phe Phe 
    370                 375                 380                 


Lys Thr Ile Asp Gly Ile Met Ala Tyr His Gln Glu Pro Pro Gly Lys 
385                 390                 395                 400 


Glu Asn Glu Thr Asp Arg Glu Ala Gly Lys Asn Ile Thr Leu Asp Ile 
                405                 410                 415     


Met Arg Glu Lys Val Pro Tyr Ile Tyr Arg Lys Leu Leu Pro Ile Glu 
            420                 425                 430         


Asp Ser His Ile Asn Arg Val Pro Leu Val Ser Ile Tyr Ile Pro Ala 
        435                 440                 445             


Tyr Asn Cys Ala Asn Tyr Ile Gln Arg Cys Val Asp Ser Ala Leu Asn 
    450                 455                 460                 


Gln Thr Val Val Asp Leu Glu Val Cys Ile Cys Asn Asp Gly Ser Thr 
465                 470                 475                 480 


Asp Asn Thr Leu Glu Val Ile Asn Lys Leu Tyr Gly Asn Asn Pro Arg 
                485                 490                 495     


Val Arg Ile Met Ser Lys Pro Asn Gly Gly Ile Ala Ser Ala Ser Asn 
            500                 505                 510         


Ala Ala Val Ser Phe Ala Lys Gly Tyr Tyr Ile Gly Gln Leu Asp Ser 
        515                 520                 525             


Asp Asp Tyr Leu Glu Pro Asp Ala Val Glu Leu Cys Leu Lys Glu Phe 
    530                 535                 540                 


Leu Lys Asp Lys Thr Leu Ala Cys Val Tyr Thr Thr Asn Arg Asn Val 
545                 550                 555                 560 


Asn Pro Asp Gly Ser Leu Ile Ala Asn Gly Tyr Asn Trp Pro Glu Phe 
                565                 570                 575     


Ser Arg Glu Lys Leu Thr Thr Ala Met Ile Ala His His Phe Arg Met 
            580                 585                 590         


Phe Thr Ile Arg Ala Trp His Leu Thr Asp Gly Phe Asn Glu Lys Ile 
        595                 600                 605             


Glu Asn Ala Val Asp Tyr Asp Met Phe Leu Lys Leu Ser Glu Val Gly 
    610                 615                 620                 


Lys Phe Lys His Leu Asn Lys Ile Cys Tyr Asn Arg Val Leu His Gly 
625                 630                 635                 640 


Asp Asn Thr Ser Ile Lys Lys Leu Gly Ile Gln Lys Lys Asn His Phe 
                645                 650                 655     


Val Val Val Asn Gln Ser Leu Asn Arg Gln Gly Ile Thr Tyr Tyr Asn 
            660                 665                 670         


Tyr Asp Glu Phe Asp Asp Leu Asp Glu Ser Arg Lys Tyr Ile Phe Asn 
        675                 680                 685             


Lys Thr Ala Glu Tyr Gln Glu Glu Ile Asp Ile Leu Lys Asp Ile Lys 
    690                 695                 700                 


Ile Ile Gln Asn Lys Asp Ala Lys Ile Ala Val Ser Ile Phe Tyr Pro 
705                 710                 715                 720 


Asn Thr Leu Asn Gly Leu Val Lys Lys Leu Asn Asn Ile Ile Glu Tyr 
                725                 730                 735     


Asn Lys Asn Ile Phe Val Ile Val Leu His Val Asp Lys Asn His Leu 
            740                 745                 750         


Thr Pro Asp Ile Lys Lys Glu Ile Leu Ala Phe Tyr His Lys His Gln 
        755                 760                 765             


Val Asn Ile Leu Leu Asn Asn Asp Ile Ser Tyr Tyr Thr Ser Asn Arg 
    770                 775                 780                 


Leu Ile Lys Thr Glu Ala His Leu Ser Asn Ile Asn Lys Leu Ser Gln 
785                 790                 795                 800 


Leu Asn Leu Asn Cys Glu Tyr Ile Ile Phe Asp Asn His Asp Ser Leu 
                805                 810                 815     


Phe Val Lys Asn Asp Ser Tyr Ala Tyr Met Lys Lys Tyr Asp Val Gly 
            820                 825                 830         


Met Asn Phe Ser Ala Leu Thr His Asp Trp Ile Glu Lys Ile Asn Ala 
        835                 840                 845             


His Pro Pro Phe Lys Lys Leu Ile Lys Thr Tyr Phe Asn Asp Asn Asp 
    850                 855                 860                 


Leu Lys Ser Met Asn Val Lys Gly Ala Ser Gln Gly Met Phe Met Thr 
865                 870                 875                 880 


Tyr Ala Leu Ala His Glu Leu Leu Thr Ile Ile Lys Glu Val Ile Thr 
                885                 890                 895     


Ser Cys Gln Ser Ile Asp Ser Val Pro Glu Tyr Asn Thr Glu Asp Ile 
            900                 905                 910         


Trp Phe Gln Phe Ala Leu Leu Ile Leu Glu Lys Lys Thr Gly His Val 
        915                 920                 925             


Phe Asn Lys Thr Ser Thr Leu Thr Tyr Met Pro Trp Glu Arg Lys Leu 
    930                 935                 940                 


Gln Trp Thr Asn Glu Gln Ile Glu Ser Ala Lys Arg Gly Glu Asn Ile 
945                 950                 955                 960 


Pro Val Asn Lys Phe Ile Ile Asn Ser Ile Thr Leu 
                965                 970         


<210>  3
<211>  2694
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pm 1062 (delta)hyaD (BglII fragment deleted) nucleotide sequence

<400>  3
atgaatacat tatcacaagc aataaaagca tataacagca atgactatca attagcactc       60

aaattatttg aaaagtcggc ggaaatctat ggacggaaaa ttgttgaatt tcaaattacc      120

aaatgcaaag aaaaactctc agcacatcct tctgttaatt cagcacatct ttctgtaaat      180

aaagaagaaa aagtcaatgt ttgcgatagt ccgttagata ttgcaacaca actgttactt      240

tccaacgtaa aaaaattagt actttctgac tcggaaaaaa acacgttaaa aaataaatgg      300

aaattgctca ctgagaagaa atctgaaaat gcggaggtaa gagcggtcgc ccttgtacca      360

aaagattttc ccaaagatct atcaccgatc attcgccaat atgaaaataa attggatatt      420

cgctacgtca gacaaaaaga taacggtttt caagccagtg ccgctcggaa tatgggatta      480

cgcttagcaa aatatgactt tattggctta ctcgactgtg atatggcgcc aaatccatta      540

tgggttcatt cttatgttgc agagctatta gaagatgatg atttaacaat cattggtcca      600

agaaaataca tcgatacaca acatattgac ccaaaagact tcttaaataa cgcgagtttg      660

cttgaatcat taccagaagt gaaaaccaat aatagtgttg ccgcaaaagg ggaaggaaca      720

gtttctctgg attggcgctt agaacaattc gaaaaaacag aaaatctccg cttatccgat      780

tcgcctttcc gtttttttgc ggcgggtaat gttgctttcg ctaaaaaatg gctaaataaa      840

tccggtttct ttgatgagga atttaatcac tggggtggag aagatgtgga atttggatat      900

cgcttattcc gttacggtag tttctttaaa actattgatg gcattatggc ctaccatcaa      960

gagccaccag gtaaagaaaa tgaaaccgat cgtgaagcgg gaaaaaatat tacgctcgat     1020

attatgagag aaaaggtccc ttatatctat agaaaacttt taccaataga agattcgcat     1080

atcaatagag tacctttagt ttcaatttat atcccagctt ataactgtgc aaactatatt     1140

caacgttgcg tagatagtgc actgaatcag actgttgttg atctcgaggt ttgtatttgt     1200

aacgatggtt caacagataa taccttagaa gtgatcaata agctttatgg taataatcct     1260

agggtacgca tcatgtctaa accaaatggc ggaatagcct cagcatcaaa tgcagccgtt     1320

tcttttgcta aaggttatta cattgggcag ttagattcag atgattatct tgagcctgat     1380

gcagttgaac tgtgtttaaa agaattttta aaagataaaa cgctagcttg tgtttatacc     1440

actaatagaa acgtcaatcc ggatggtagc ttaatcgcta atggttacaa ttggccagaa     1500

ttttcacgag aaaaactcac aacggctatg attgctcacc actttagaat gttcacgatt     1560

agagcttggc atttaactga tggattcaat gaaaaaattg aaaatgccgt agactatgac     1620

atgttcctca aactcagtga agttggaaaa tttaaacatc ttaataaaat ctgctataac     1680

cgtgtattac atggtgataa cacatcaatt aagaaacttg gcattcaaaa gaaaaaccat     1740

tttgttgtag tcaatcagtc attaaataga caaggcataa cttattataa ttatgacgaa     1800

tttgatgatt tagatgaaag tagaaagtat attttcaata aaaccgctga atatcaagaa     1860

gagattgata tcttaaaaga tattaaaatc atccagaata aagatgccaa aatcgcagtc     1920

agtatttttt atcccaatac attaaacggc ttagtgaaaa aactaaacaa tattattgaa     1980

tataataaaa atatattcgt tattgttcta catgttgata agaatcatct tacaccagat     2040

atcaaaaaag aaatactagc cttctatcat aaacatcaag tgaatatttt actaaataat     2100

gatatctcat attacacgag taatagatta ataaaaactg aggcgcattt aagtaatatt     2160

aataaattaa gtcagttaaa tctaaattgt gaatacatca tttttgataa tcatgacagc     2220

ctattcgtta aaaatgacag ctatgcttat atgaaaaaat atgatgtcgg catgaatttc     2280

tcagcattaa cacatgattg gatcgagaaa atcaatgcgc atccaccatt taaaaagctc     2340

attaaaactt attttaatga caatgactta aaaagtatga atgtgaaagg ggcatcacaa     2400

ggtatgttta tgacgtatgc gctagcgcat gagcttctga cgattattaa agaagtcatc     2460

acatcttgcc agtcaattga tagtgtgcca gaatataaca ctgaggatat ttggttccaa     2520

tttgcacttt taatcttaga aaagaaaacc ggccatgtat ttaataaaac atcgaccctg     2580

acttatatgc cttgggaacg aaaattacaa tggacaaatg aacaaattga aagtgcaaaa     2640

agaggagaaa atatacctgt taacaagttc attattaata gtataactct ataa           2694


<210>  4
<211>  897
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pm 1062 (delta)hyaD (BglII fragment deleted) amino acid sequence

<400>  4

Met Asn Thr Leu Ser Gln Ala Ile Lys Ala Tyr Asn Ser Asn Asp Tyr 
1               5                   10                  15      


Gln Leu Ala Leu Lys Leu Phe Glu Lys Ser Ala Glu Ile Tyr Gly Arg 
            20                  25                  30          


Lys Ile Val Glu Phe Gln Ile Thr Lys Cys Lys Glu Lys Leu Ser Ala 
        35                  40                  45              


His Pro Ser Val Asn Ser Ala His Leu Ser Val Asn Lys Glu Glu Lys 
    50                  55                  60                  


Val Asn Val Cys Asp Ser Pro Leu Asp Ile Ala Thr Gln Leu Leu Leu 
65                  70                  75                  80  


Ser Asn Val Lys Lys Leu Val Leu Ser Asp Ser Glu Lys Asn Thr Leu 
                85                  90                  95      


Lys Asn Lys Trp Lys Leu Leu Thr Glu Lys Lys Ser Glu Asn Ala Glu 
            100                 105                 110         


Val Arg Ala Val Ala Leu Val Pro Lys Asp Phe Pro Lys Asp Leu Ser 
        115                 120                 125             


Pro Ile Ile Arg Gln Tyr Glu Asn Lys Leu Asp Ile Arg Tyr Val Arg 
    130                 135                 140                 


Gln Lys Asp Asn Gly Phe Gln Ala Ser Ala Ala Arg Asn Met Gly Leu 
145                 150                 155                 160 


Arg Leu Ala Lys Tyr Asp Phe Ile Gly Leu Leu Asp Cys Asp Met Ala 
                165                 170                 175     


Pro Asn Pro Leu Trp Val His Ser Tyr Val Ala Glu Leu Leu Glu Asp 
            180                 185                 190         


Asp Asp Leu Thr Ile Ile Gly Pro Arg Lys Tyr Ile Asp Thr Gln His 
        195                 200                 205             


Ile Asp Pro Lys Asp Phe Leu Asn Asn Ala Ser Leu Leu Glu Ser Leu 
    210                 215                 220                 


Pro Glu Val Lys Thr Asn Asn Ser Val Ala Ala Lys Gly Glu Gly Thr 
225                 230                 235                 240 


Val Ser Leu Asp Trp Arg Leu Glu Gln Phe Glu Lys Thr Glu Asn Leu 
                245                 250                 255     


Arg Leu Ser Asp Ser Pro Phe Arg Phe Phe Ala Ala Gly Asn Val Ala 
            260                 265                 270         


Phe Ala Lys Lys Trp Leu Asn Lys Ser Gly Phe Phe Asp Glu Glu Phe 
        275                 280                 285             


Asn His Trp Gly Gly Glu Asp Val Glu Phe Gly Tyr Arg Leu Phe Arg 
    290                 295                 300                 


Tyr Gly Ser Phe Phe Lys Thr Ile Asp Gly Ile Met Ala Tyr His Gln 
305                 310                 315                 320 


Glu Pro Pro Gly Lys Glu Asn Glu Thr Asp Arg Glu Ala Gly Lys Asn 
                325                 330                 335     


Ile Thr Leu Asp Ile Met Arg Glu Lys Val Pro Tyr Ile Tyr Arg Lys 
            340                 345                 350         


Leu Leu Pro Ile Glu Asp Ser His Ile Asn Arg Val Pro Leu Val Ser 
        355                 360                 365             


Ile Tyr Ile Pro Ala Tyr Asn Cys Ala Asn Tyr Ile Gln Arg Cys Val 
    370                 375                 380                 


Asp Ser Ala Leu Asn Gln Thr Val Val Asp Leu Glu Val Cys Ile Cys 
385                 390                 395                 400 


Asn Asp Gly Ser Thr Asp Asn Thr Leu Glu Val Ile Asn Lys Leu Tyr 
                405                 410                 415     


Gly Asn Asn Pro Arg Val Arg Ile Met Ser Lys Pro Asn Gly Gly Ile 
            420                 425                 430         


Ala Ser Ala Ser Asn Ala Ala Val Ser Phe Ala Lys Gly Tyr Tyr Ile 
        435                 440                 445             


Gly Gln Leu Asp Ser Asp Asp Tyr Leu Glu Pro Asp Ala Val Glu Leu 
    450                 455                 460                 


Cys Leu Lys Glu Phe Leu Lys Asp Lys Thr Leu Ala Cys Val Tyr Thr 
465                 470                 475                 480 


Thr Asn Arg Asn Val Asn Pro Asp Gly Ser Leu Ile Ala Asn Gly Tyr 
                485                 490                 495     


Asn Trp Pro Glu Phe Ser Arg Glu Lys Leu Thr Thr Ala Met Ile Ala 
            500                 505                 510         


His His Phe Arg Met Phe Thr Ile Arg Ala Trp His Leu Thr Asp Gly 
        515                 520                 525             


Phe Asn Glu Lys Ile Glu Asn Ala Val Asp Tyr Asp Met Phe Leu Lys 
    530                 535                 540                 


Leu Ser Glu Val Gly Lys Phe Lys His Leu Asn Lys Ile Cys Tyr Asn 
545                 550                 555                 560 


Arg Val Leu His Gly Asp Asn Thr Ser Ile Lys Lys Leu Gly Ile Gln 
                565                 570                 575     


Lys Lys Asn His Phe Val Val Val Asn Gln Ser Leu Asn Arg Gln Gly 
            580                 585                 590         


Ile Thr Tyr Tyr Asn Tyr Asp Glu Phe Asp Asp Leu Asp Glu Ser Arg 
        595                 600                 605             


Lys Tyr Ile Phe Asn Lys Thr Ala Glu Tyr Gln Glu Glu Ile Asp Ile 
    610                 615                 620                 


Leu Lys Asp Ile Lys Ile Ile Gln Asn Lys Asp Ala Lys Ile Ala Val 
625                 630                 635                 640 


Ser Ile Phe Tyr Pro Asn Thr Leu Asn Gly Leu Val Lys Lys Leu Asn 
                645                 650                 655     


Asn Ile Ile Glu Tyr Asn Lys Asn Ile Phe Val Ile Val Leu His Val 
            660                 665                 670         


Asp Lys Asn His Leu Thr Pro Asp Ile Lys Lys Glu Ile Leu Ala Phe 
        675                 680                 685             


Tyr His Lys His Gln Val Asn Ile Leu Leu Asn Asn Asp Ile Ser Tyr 
    690                 695                 700                 


Tyr Thr Ser Asn Arg Leu Ile Lys Thr Glu Ala His Leu Ser Asn Ile 
705                 710                 715                 720 


Asn Lys Leu Ser Gln Leu Asn Leu Asn Cys Glu Tyr Ile Ile Phe Asp 
                725                 730                 735     


Asn His Asp Ser Leu Phe Val Lys Asn Asp Ser Tyr Ala Tyr Met Lys 
            740                 745                 750         


Lys Tyr Asp Val Gly Met Asn Phe Ser Ala Leu Thr His Asp Trp Ile 
        755                 760                 765             


Glu Lys Ile Asn Ala His Pro Pro Phe Lys Lys Leu Ile Lys Thr Tyr 
    770                 775                 780                 


Phe Asn Asp Asn Asp Leu Lys Ser Met Asn Val Lys Gly Ala Ser Gln 
785                 790                 795                 800 


Gly Met Phe Met Thr Tyr Ala Leu Ala His Glu Leu Leu Thr Ile Ile 
                805                 810                 815     


Lys Glu Val Ile Thr Ser Cys Gln Ser Ile Asp Ser Val Pro Glu Tyr 
            820                 825                 830         


Asn Thr Glu Asp Ile Trp Phe Gln Phe Ala Leu Leu Ile Leu Glu Lys 
        835                 840                 845             


Lys Thr Gly His Val Phe Asn Lys Thr Ser Thr Leu Thr Tyr Met Pro 
    850                 855                 860                 


Trp Glu Arg Lys Leu Gln Trp Thr Asn Glu Gln Ile Glu Ser Ala Lys 
865                 870                 875                 880 


Arg Gly Glu Asn Ile Pro Val Asn Lys Phe Ile Ile Asn Ser Ile Thr 
                885                 890                 895     


Leu 
    


<210>  5
<211>  4000
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wild-type 1062 nanPU nucleotide sequence (prior to deletion)

<400>  5
gtaatcccaa cgtaaccaat agaggagaac tcataatgaa atttaaaaaa ctactacttg       60

catctttatg tttaggtgtt tcagcttctg tatttgcagc agattacgat cttaaattcg      120

gtatggttgc gggtccaagc tcaaacgaat ataaagcagt agaattcttt gcgaaagaag      180

tgaaagaaaa atccaatggc aaaattgatg tggctatttt ccctagctca cagttaggtg      240

atgaccgtgt gatgattaaa caattaaaag acggtgcatt agactttacg ttaggtgaat      300

cagcacgttt ccaaatttac ttcccagaag cagaagtatt tgcgttgcct tatatgattc      360

ctaattttga aacctctaaa aaagcgttgc tcgacacaaa atttggtcaa ggtttattga      420

aaaaaattga taaagagtta aacgtacaag tgttatctgt ggcgtataac ggtacacgtc      480

aaacaacttc taaccgtgca atcaacagca ttgaagacat gaaagggtta aaattacgtg      540

tacctaacgc ggcaaccaac cttgcttatg caaaatacgt gggtgcagcg ccaacaccaa      600

tggcattctc tgaagtttac cttgcgcttc aaacaaactc tgtggatggt caagaaaacc      660

cattaccgac aatccaagca caaaaattct atgaagtaca aaaatactta gcgttaacta      720

accacatctt aaatgaccaa ctttacttaa tcagtaacga tacgttggca gatttaccag      780

aagatttaca aaaagtggtt aaagatgcag cagcgaaagc cgctgaatat cacactaaac      840

tcttcgttga cggtgagaac agcttagttg aattcttcaa aagtcaaggt gtgacagtca      900

cacaaccaga cttaaaacca tttaaagcag cacttacacc atactatgat gaatatctca      960

agaaaaatgg tgaagtcggt aaaatggcga ttgaagaaat ttctaatctc gctaaataaa     1020

tatagtaacc ttatccctgc gccttaaggg ataaggttcc tttttattgg gttgtcttga     1080

ggtatctatg aaaataataa ataaattaga agagtggatt ggcggtgtgc tattcattgg     1140

aattttctta attctgttag cacaaatcat tgctcgtcaa gtgtttcagt caccgtttat     1200

ttggagtgaa gaactcgcaa gattgctatt tatctatgtc gggctacttg gtatcagcat     1260

gggtatccgt agtcagcagc atgtttatat tgatttttta actaacttta tgcccgagaa     1320

agtgagaaag gtgacaaact cctttgttca agttctcatc tttatttcca tcattatttt     1380

cattcattta ggctttaaag tttggatcga ctccagtttt aaaatggaag cgttaactgc     1440

tttcgcttca gatttaattg ggcgcgagac gattgtgcct gaaaaatgga tgtatgcggc     1500

attgcctttt atttcttgtt taatgttatt ccgctttttc caagcgcaag ttgaaaatta     1560

tagaaataag ttaagttata ttcctgtcac ggcatttgtg attggtgcgg tcattatttt     1620

tgcgatttta ttgattgagc cagattggta taaagtcctc cgtatttcaa attatgtgaa     1680

atttggtggt gatgcagtgt atatcacatt agtgatttgg cttgtcatta tgtttgtggg     1740

aaccccggta ggttggtcat tatttattgc gacgttgctt tattttgcga tgacgcgttg     1800

gaatattgtt aactcggcat caaccaagct caccgacagt ttaaatagtt tcccattatt     1860

gagtgtgccg ttctttattt taaccggtat tttaatgaat acgggcggaa ttacagaacg     1920

tatttttgat ttcgcacgtg ccttgctcgg tcattaccgt ggtggtatgg gacacgtgaa     1980

tatcggggca agtttaattt tctcaggtat gtctggttct gcacttgccg atgcaggtgg     2040

tttaggccag ttagaaatta aagccatgcg tgatgctggg tatgacgatg acatctgtgg     2100

tgggattacc gctgcttctt gtattatcgg tccattagtt ccaccaagta ttgcgatgat     2160

tatctatggg gttatttcta accaatctat tgcaaaatta tttattgcgg gttttattcc     2220

tggtgtgctc gtaaccattg cgttaatgat catgaactat tatgtggcga aaaaacgtgg     2280

ttatccaaga acacctaaag cgacccttga acaacgttgt caggcattta aaaaggccat     2340

ttgggcagtg ttaaccccaa ttttgattat cggtggtatt ttctctggtc tctttacacc     2400

aacggaagcc gcggtgattg cagccttcta ttccattatt atcgggatgt ttgtttaccg     2460

agagttgaat ttacaaatgt tgttcaaaag ctgtattgaa gcaatggcga ttacaggggt     2520

aacagcatta atggtgatga cggtcacttt ctttggtgac atgattgcac gtgagcaagt     2580

ggctatgaaa attgcagaag tctttgttgc agtagccgat tcaccaacga tggtgttagt     2640

catgatcaac ttattgctct tgttccttgg tatgtttatt gatgctttag cattgcaatt     2700

cttggtgtta ccaatgttaa ttccaattgc ggttcacttt ggcattgact taattttctt     2760

tggtgtcatg accacattaa atatgatgat tggtattttg actccaccaa tgggaatggc     2820

attatttgtt gtggcacgtg ttggtaatat gccagtgagt acagtcgcaa aaggggtttt     2880

acctttctta gtaccaattt ttgtgacact ggtgttgatt acaattttcc cacaaattat     2940

cacctttata ccaaatcttc tgatgccata atggcgtgaa gaaatggcat tcaaagccaa     3000

tcggactcgg ttggctttaa tttaaaaaac ttgccattca gaattatgct atctgaatcg     3060

gtattcattc ttactaacct aattaattga ggtaataaaa tgaaatttac aaaaacagcg     3120

ttatttacgg tattagcagc aacggcattt gccgcacaag caggtcagta tccagattta     3180

ccagaaggca ttaaagccgg tgcaggtgca ttaattggtg ataccgttta tgtggggtta     3240

ggtggtactg gcacaacaaa attctattca ttaaatttga aagatccaaa agagtggaaa     3300

gaaattgcag aattccctgg tggtaaacgt aatcagcctg ttgctgcggg tgtgaatggt     3360

aagctttatg tgtttggtgg tttccaagat acagatgtcg cgaaaaatca aattatcaat     3420

gatgcttatg agtataatcc ggcagataat acgtggacaa aattaagcac acgttctcct     3480

cgttcaacat ctgtgggagc gagtgttgca gcagatggcg gtaaaattta cttcgtaggt     3540

ggggtaaacc acgaaatttg gaatggttta ttccaagatg ttaaagctgc aggtggtgat     3600

aaagagaaag aaaaagcgat ctttgacccg tatttcaatt tacgcgcaca agatttcttc     3660

ttctcaccag aaatcatcag ttatgagcca gctaacaatg tatggcgtaa cgaaggctac     3720

ttcccatatt cgggtcgtgc aggcgctgcg gttgcgatta aagatggtaa attattagtc     3780

gtgaatggtg aagtgaaagc aggtttacgc tcgccaggta ctgcgttagg tacgattggt     3840

aaagatggcg ttacttggaa aaaactcggt gatttaccag caccaacagg ctatgacaaa     3900

caagatggta ttgcaggcgg tatgggtggt tataccaatg gtcattatat cgtgacaggt     3960

ggtgcgaact tccctggtgc attagcaaac tatgaaaaag                           4000


<210>  6
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1062nanPU_F primer

<400>  6
ttccctagct cacagttagg tgat                                              24


<210>  7
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1062nanPU_delR primer

<400>  7
gtcacacctt gacttttgaa gaattca                                           27


<210>  8
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1062nanPU_delF primer

<400>  8
aattccaatt gcggttcact ttggca                                            26


<210>  9
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1062nanPU_R primer

<400>  9
tctgcaattt ctttccattc ttttgga                                           27


<210>  10
<211>  327
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  WILD-TYPE NANP AMINO ACID SEQUENCE

<400>  10

Met Lys Phe Lys Lys Leu Leu Leu Ala Ser Leu Cys Leu Gly Val Ser 
1               5                   10                  15      


Ala Ser Val Phe Ala Ala Asp Tyr Asp Leu Lys Phe Gly Met Val Ala 
            20                  25                  30          


Gly Pro Ser Ser Asn Glu Tyr Lys Ala Val Glu Phe Phe Ala Lys Glu 
        35                  40                  45              


Val Lys Glu Lys Ser Asn Gly Lys Ile Asp Val Ala Ile Phe Pro Ser 
    50                  55                  60                  


Ser Gln Leu Gly Asp Asp Arg Val Met Ile Lys Gln Leu Lys Asp Gly 
65                  70                  75                  80  


Ala Leu Asp Phe Thr Leu Gly Glu Ser Ala Arg Phe Gln Ile Tyr Phe 
                85                  90                  95      


Pro Glu Ala Glu Val Phe Ala Leu Pro Tyr Met Ile Pro Asn Phe Glu 
            100                 105                 110         


Thr Ser Lys Lys Ala Leu Leu Asp Thr Lys Phe Gly Gln Gly Leu Leu 
        115                 120                 125             


Lys Lys Ile Asp Lys Glu Leu Asn Val Gln Val Leu Ser Val Ala Tyr 
    130                 135                 140                 


Asn Gly Thr Arg Gln Thr Thr Ser Asn Arg Ala Ile Asn Ser Ile Glu 
145                 150                 155                 160 


Asp Met Lys Gly Leu Lys Leu Arg Val Pro Asn Ala Ala Thr Asn Leu 
                165                 170                 175     


Ala Tyr Ala Lys Tyr Val Gly Ala Ala Pro Thr Pro Met Ala Phe Ser 
            180                 185                 190         


Glu Val Tyr Leu Ala Leu Gln Thr Asn Ser Val Asp Gly Gln Glu Asn 
        195                 200                 205             


Pro Leu Pro Thr Ile Gln Ala Gln Lys Phe Tyr Glu Val Gln Lys Tyr 
    210                 215                 220                 


Leu Ala Leu Thr Asn His Ile Leu Asn Asp Gln Leu Tyr Leu Ile Ser 
225                 230                 235                 240 


Asn Asp Thr Leu Ala Asp Leu Pro Glu Asp Leu Gln Lys Val Val Lys 
                245                 250                 255     


Asp Ala Ala Ala Lys Ala Ala Glu Tyr His Thr Lys Leu Phe Val Asp 
            260                 265                 270         


Gly Glu Asn Ser Leu Val Glu Phe Phe Lys Ser Gln Gly Val Thr Val 
        275                 280                 285             


Thr Gln Pro Asp Leu Lys Pro Phe Lys Ala Ala Leu Thr Pro Tyr Tyr 
    290                 295                 300                 


Asp Glu Tyr Leu Lys Lys Asn Gly Glu Val Gly Lys Met Ala Ile Glu 
305                 310                 315                 320 


Glu Ile Ser Asn Leu Ala Lys 
                325         


<210>  11
<211>  627
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  WILD-TYPE NANU AMINO ACID SEQUENCE

<400>  11

Met Lys Ile Ile Asn Lys Leu Glu Glu Trp Ile Gly Gly Val Leu Phe 
1               5                   10                  15      


Ile Gly Ile Phe Leu Ile Leu Leu Ala Gln Ile Ile Ala Arg Gln Val 
            20                  25                  30          


Phe Gln Ser Pro Phe Ile Trp Ser Glu Glu Leu Ala Arg Leu Leu Phe 
        35                  40                  45              


Ile Tyr Val Gly Leu Leu Gly Ile Ser Met Gly Ile Arg Ser Gln Gln 
    50                  55                  60                  


His Val Tyr Ile Asp Phe Leu Thr Asn Phe Met Pro Glu Lys Val Arg 
65                  70                  75                  80  


Lys Val Thr Asn Ser Phe Val Gln Val Leu Ile Phe Ile Ser Ile Ile 
                85                  90                  95      


Ile Phe Ile His Leu Gly Phe Lys Val Trp Ile Asp Ser Ser Phe Lys 
            100                 105                 110         


Met Glu Ala Leu Thr Ala Phe Ala Ser Asp Leu Ile Gly Arg Glu Thr 
        115                 120                 125             


Ile Val Pro Glu Lys Trp Met Tyr Ala Ala Leu Pro Phe Ile Ser Cys 
    130                 135                 140                 


Leu Met Leu Phe Arg Phe Phe Gln Ala Gln Val Glu Asn Tyr Arg Asn 
145                 150                 155                 160 


Lys Leu Ser Tyr Ile Pro Val Thr Ala Phe Val Ile Gly Ala Val Ile 
                165                 170                 175     


Ile Phe Ala Ile Leu Leu Ile Glu Pro Asp Trp Tyr Lys Val Leu Arg 
            180                 185                 190         


Ile Ser Asn Tyr Val Lys Phe Gly Gly Asp Ala Val Tyr Ile Thr Leu 
        195                 200                 205             


Val Ile Trp Leu Val Ile Met Phe Val Gly Thr Pro Val Gly Trp Ser 
    210                 215                 220                 


Leu Phe Ile Ala Thr Leu Leu Tyr Phe Ala Met Thr Arg Trp Asn Ile 
225                 230                 235                 240 


Val Asn Ser Ala Ser Thr Lys Leu Thr Asp Ser Leu Asn Ser Phe Pro 
                245                 250                 255     


Leu Leu Ser Val Pro Phe Phe Ile Leu Thr Gly Ile Leu Met Asn Thr 
            260                 265                 270         


Gly Gly Ile Thr Glu Arg Ile Phe Asp Phe Ala Arg Ala Leu Leu Gly 
        275                 280                 285             


His Tyr Arg Gly Gly Met Gly His Val Asn Ile Gly Ala Ser Leu Ile 
    290                 295                 300                 


Phe Ser Gly Met Ser Gly Ser Ala Leu Ala Asp Ala Gly Gly Leu Gly 
305                 310                 315                 320 


Gln Leu Glu Ile Lys Ala Met Arg Asp Ala Gly Tyr Asp Asp Asp Ile 
                325                 330                 335     


Cys Gly Gly Ile Thr Ala Ala Ser Cys Ile Ile Gly Pro Leu Val Pro 
            340                 345                 350         


Pro Ser Ile Ala Met Ile Ile Tyr Gly Val Ile Ser Asn Gln Ser Ile 
        355                 360                 365             


Ala Lys Leu Phe Ile Ala Gly Phe Ile Pro Gly Val Leu Val Thr Ile 
    370                 375                 380                 


Ala Leu Met Ile Met Asn Tyr Tyr Val Ala Lys Lys Arg Gly Tyr Pro 
385                 390                 395                 400 


Arg Thr Pro Lys Ala Thr Leu Glu Gln Arg Cys Gln Ala Phe Lys Lys 
                405                 410                 415     


Ala Ile Trp Ala Val Leu Thr Pro Ile Leu Ile Ile Gly Gly Ile Phe 
            420                 425                 430         


Ser Gly Leu Phe Thr Pro Thr Glu Ala Ala Val Ile Ala Ala Phe Tyr 
        435                 440                 445             


Ser Ile Ile Ile Gly Met Phe Val Tyr Arg Glu Leu Asn Leu Gln Met 
    450                 455                 460                 


Leu Phe Lys Ser Cys Ile Glu Ala Met Ala Ile Thr Gly Val Thr Ala 
465                 470                 475                 480 


Leu Met Val Met Thr Val Thr Phe Phe Gly Asp Met Ile Ala Arg Glu 
                485                 490                 495     


Gln Val Ala Met Lys Ile Ala Glu Val Phe Val Ala Val Ala Asp Ser 
            500                 505                 510         


Pro Thr Met Val Leu Val Met Ile Asn Leu Leu Leu Leu Phe Leu Gly 
        515                 520                 525             


Met Phe Ile Asp Ala Leu Ala Leu Gln Phe Leu Val Leu Pro Met Leu 
    530                 535                 540                 


Ile Pro Ile Ala Val His Phe Gly Ile Asp Leu Ile Phe Phe Gly Val 
545                 550                 555                 560 


Met Thr Thr Leu Asn Met Met Ile Gly Ile Leu Thr Pro Pro Met Gly 
                565                 570                 575     


Met Ala Leu Phe Val Val Ala Arg Val Gly Asn Met Pro Val Ser Thr 
            580                 585                 590         


Val Ala Lys Gly Val Leu Pro Phe Leu Val Pro Ile Phe Val Thr Leu 
        595                 600                 605             


Val Leu Ile Thr Ile Phe Pro Gln Ile Ile Thr Phe Ile Pro Asn Leu 
    610                 615                 620                 


Leu Met Pro 
625         


<210>  12
<211>  2146
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant 1062 nanpu

<400>  12
gtaatcccaa cgtaaccaat agaggagaac tcataatgaa atttaaaaaa ctactacttg       60

catctttatg tttaggtgtt tcagcttctg tatttgcagc agattacgat cttaaattcg      120

gtatggttgc gggtccaagc tcaaacgaat ataaagcagt agaattcttt gcgaaagaag      180

tgaaagaaaa atccaatggc aaaattgatg tggctatttt ccctagctca cagttaggtg      240

atgaccgtgt gatgattaaa caattaaaag acggtgcatt agactttacg ttaggtgaat      300

cagcacgttt ccaaatttac ttcccagaag cagaagtatt tgcgttgcct tatatgattc      360

ctaattttga aacctctaaa aaagcgttgc tcgacacaaa atttggtcaa ggtttattga      420

aaaaaattga taaagagtta aacgtacaag tgttatctgt ggcgtataac ggtacacgtc      480

aaacaacttc taaccgtgca atcaacagca ttgaagacat gaaagggtta aaattacgtg      540

tacctaacgc ggcaaccaac cttgcttatg caaaatacgt gggtgcagcg ccaacaccaa      600

tggcattctc tgaagtttac cttgcgcttc aaacaaactc tgtggatggt caagaaaacc      660

cattaccgac aatccaagca caaaaattct atgaagtaca aaaatactta gcgttaacta      720

accacatctt aaatgaccaa ctttacttaa tcagtaacga tacgttggca gatttaccag      780

aagatttaca aaaagtggtt aaagatgcag cagcgaaagc cgctgaatat cacactaaac      840

tcttcgttga cggtgagaac agcttagttg aattgcggtt cactttggca ttgacttaat      900

tttctttggt gtcatgacca cattaaatat gatgattggt attttgactc caccaatggg      960

aatggcatta tttgttgtgg cacgtgttgg taatatgcca gtgagtacag tcgcaaaagg     1020

ggttttacct ttcttagtac caatttttgt gacactggtg ttgattacaa ttttcccaca     1080

aattatcacc tttataccaa atcttctgat gccataatgg cgtgaagaaa tggcattcaa     1140

agccaatcgg actcggttgg ctttaattta aaaaacttgc cattcagaat tatgctatct     1200

gaatcggtat tcattcttac taacctaatt aattgaggta ataaaatgaa atttacaaaa     1260

acagcgttat ttacggtatt agcagcaacg gcatttgccg cacaagcagg tcagtatcca     1320

gatttaccag aaggcattaa agccggtgca ggtgcattaa ttggtgatac cgtttatgtg     1380

gggttaggtg gtactggcac aacaaaattc tattcattaa atttgaaaga tccaaaagag     1440

tggaaagaaa ttgcagaatt ccctggtggt aaacgtaatc agcctgttgc tgcgggtgtg     1500

aatggtaagc tttatgtgtt tggtggtttc caagatacag atgtcgcgaa aaatcaaatt     1560

atcaatgatg cttatgagta taatccggca gataatacgt ggacaaaatt aagcacacgt     1620

tctcctcgtt caacatctgt gggagcgagt gttgcagcag atggcggtaa aatttacttc     1680

gtaggtgggg taaaccacga aatttggaat ggtttattcc aagatgttaa agctgcaggt     1740

ggtgataaag agaaagaaaa agcgatcttt gacccgtatt tcaatttacg cgcacaagat     1800

ttcttcttct caccagaaat catcagttat gagccagcta acaatgtatg gcgtaacgaa     1860

ggctacttcc catattcggg tcgtgcaggc gctgcggttg cgattaaaga tggtaaatta     1920

ttagtcgtga atggtgaagt gaaagcaggt ttacgctcgc caggtactgc gttaggtacg     1980

attggtaaag atggcgttac ttggaaaaaa ctcggtgatt taccagcacc aacaggctat     2040

gacaaacaag atggtattgc aggcggtatg ggtggttata ccaatggtca ttatatcgtg     2100

acaggtggtg cgaacttccc tggtgcatta gcaaactatg aaaaag                    2146


<210>  13
<211>  287
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MUTANT NANP AMINO ACID SEQUENCE (DEDUCED)

<400>  13

Met Lys Phe Lys Lys Leu Leu Leu Ala Ser Leu Cys Leu Gly Val Ser 
1               5                   10                  15      


Ala Ser Val Phe Ala Ala Asp Tyr Asp Leu Lys Phe Gly Met Val Ala 
            20                  25                  30          


Gly Pro Ser Ser Asn Glu Tyr Lys Ala Val Glu Phe Phe Ala Lys Glu 
        35                  40                  45              


Val Lys Glu Lys Ser Asn Gly Lys Ile Asp Val Ala Ile Phe Pro Ser 
    50                  55                  60                  


Ser Gln Leu Gly Asp Asp Arg Val Met Ile Lys Gln Leu Lys Asp Gly 
65                  70                  75                  80  


Ala Leu Asp Phe Thr Leu Gly Glu Ser Ala Arg Phe Gln Ile Tyr Phe 
                85                  90                  95      


Pro Glu Ala Glu Val Phe Ala Leu Pro Tyr Met Ile Pro Asn Phe Glu 
            100                 105                 110         


Thr Ser Lys Lys Ala Leu Leu Asp Thr Lys Phe Gly Gln Gly Leu Leu 
        115                 120                 125             


Lys Lys Ile Asp Lys Glu Leu Asn Val Gln Val Leu Ser Val Ala Tyr 
    130                 135                 140                 


Asn Gly Thr Arg Gln Thr Thr Ser Asn Arg Ala Ile Asn Ser Ile Glu 
145                 150                 155                 160 


Asp Met Lys Gly Leu Lys Leu Arg Val Pro Asn Ala Ala Thr Asn Leu 
                165                 170                 175     


Ala Tyr Ala Lys Tyr Val Gly Ala Ala Pro Thr Pro Met Ala Phe Ser 
            180                 185                 190         


Glu Val Tyr Leu Ala Leu Gln Thr Asn Ser Val Asp Gly Gln Glu Asn 
        195                 200                 205             


Pro Leu Pro Thr Ile Gln Ala Gln Lys Phe Tyr Glu Val Gln Lys Tyr 
    210                 215                 220                 


Leu Ala Leu Thr Asn His Ile Leu Asn Asp Gln Leu Tyr Leu Ile Ser 
225                 230                 235                 240 


Asn Asp Thr Leu Ala Asp Leu Pro Glu Asp Leu Gln Lys Val Val Lys 
                245                 250                 255     


Asp Ala Ala Ala Lys Ala Ala Glu Tyr His Thr Lys Leu Phe Val Asp 
            260                 265                 270         


Gly Glu Asn Ser Leu Val Glu Leu Arg Phe Thr Leu Ala Leu Thr 
        275                 280                 285         


<210>  14
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p. multocida 1062 hyad forward primer

<400>  14
atgatatttg agaagtcggc gg                                                22


<210>  15
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p. multocida 1062 hyad reverse primer

<400>  15
tgtaattttc gttcccaagg c                                                 21


<210>  16
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nanpur screening/sequencing primer

<400>  16
tctgcaattt ctttccattc ttttggatct                                        30


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hyadr screening primer

<400>  17
gtaattttcg ttcccaaggc                                                   20


