﻿               SEQUENCE LISTING

<110> Laboratoire français du Fractionnement et des Biotechnologies

<120> FUSION Fc-SAP

<130> S15/BR70884/DC1/BAJ/ll

<160> 46

<170> BiSSAP 1.0

<210> 1
<211> 204
<212> PRT
<213> Homo sapiens

<220> 
<221> SOURCE
<222> 1..204
<223> /mol_type="protein"
      /note="Human SAP"
      /organism="Homo sapiens"

<400> 1
His Thr Asp Leu Ser Gly Lys Val Phe Val Phe Pro Arg Glu Ser Val 
1               5                   10                   15    
Thr Asp His Val Asn Leu Ile Thr Pro Leu Glu Lys Pro Leu Gln Asn 
            20                   25                  30        
Phe Thr Leu Cys Phe Arg Ala Tyr Ser Asp Leu Ser Arg Ala Tyr Ser 
        35                   40                  45            
Leu Phe Ser Tyr Asn Thr Gln Gly Arg Asp Asn Glu Leu Leu Val Tyr 
    50                   55                  60                
Lys Glu Arg Val Gly Glu Tyr Ser Leu Tyr Ile Gly Arg His Lys Val 
65                   70                  75                  80
Thr Ser Lys Val Ile Glu Lys Phe Pro Ala Pro Val His Ile Cys Val 
                85                   90                  95    
Ser Trp Glu Ser Ser Ser Gly Ile Ala Glu Phe Trp Ile Asn Gly Thr 
            100                  105                110        
Pro Leu Val Lys Lys Gly Leu Arg Gln Gly Tyr Phe Val Glu Ala Gln 
        115                  120                125            
Pro Lys Ile Val Leu Gly Gln Glu Gln Asp Ser Tyr Gly Gly Lys Phe 
    130                  135                140                
Asp Arg Ser Gln Ser Phe Val Gly Glu Ile Gly Asp Leu Tyr Met Trp 
145                  150                155                  160
Asp Ser Val Leu Pro Pro Glu Asn Ile Leu Ser Ala Tyr Gln Gly Thr 
                165                  170                175    
Pro Leu Pro Ala Asn Ile Leu Asp Trp Gln Ala Leu Asn Tyr Glu Ile 
            180                  185                190        
Arg Gly Tyr Val Ile Ile Lys Pro Leu Val Trp Val 
        195                  200                

<210> 2
<211> 612
<212> DNA
<213> Homo sapiens

<220> 
<221> source
<222> 1..612
<223> /mol_type="DNA"
      /note="Huamn SAP"
      /organism="Homo sapiens"

<400> 2
cacacagacc tcagtgggaa ggtgtttgta tttcctagag aatctgttac tgatcatgta      60

aacttgatca caccgctgga gaagcctcta cagaacttta ccttgtgttt tcgagcctat     120

agtgatctct ctcgtgccta cagcctcttc tcctacaata cccaaggcag ggataatgag     180

ctactagttt ataaagaaag agttggagag tatagtctat acattggaag acacaaagtt     240

acatccaaag ttatcgaaaa gttcccggct ccagtgcaca tctgtgtgag ctgggagtcc     300

tcatcaggta ttgctgaatt ttggatcaat gggacacctt tggtgaaaaa gggtctgcga     360

cagggttact ttgtggaagc tcagcccaag attgtcctgg ggcaggaaca ggattcctat     420

gggggcaagt ttgataggag ccagtccttt gtgggagaga ttggggattt gtacatgtgg     480

gactctgtgc tgcccccaga aaatatcctg tctgcctatc agggtacccc tctccctgcc     540

aatatcctgg actggcaggc tctgaactat gaaatcagag gatatgtcat catcaaaccc     600

ttggtgtggg tc                                                         612


<210> 3
<211> 217
<212> PRT
<213> Homo sapiens

<220> 
<221> SOURCE
<222> 1..217
<223> /mol_type="protein"
      /note="Fragment of Fc region of a human antibody"
      /organism="Homo sapiens"

<400> 3
Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
1               5                   10                   15    
Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
            20                   25                  30        
Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
        35                   40                  45            
Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
    50                   55                  60                
Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
65                   70                  75                  80
Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
                85                   90                  95    
Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
            100                  105                110        
Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met 
        115                  120                125            
Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
    130                  135                140                
Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
145                  150                155                  160
Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
                165                  170                175    
Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
            180                  185                190        
Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
        195                  200                205            
Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    210                  215        

<210> 4
<211> 654
<212> DNA
<213> Homo sapiens

<220> 
<221> source
<222> 1..654
<223> /mol_type="DNA"
      /note="Fragment of Fc region of a human antibody"
      /organism="Homo sapiens"

<400> 4
gcacctgaac tcctgggggg accgtcagtc ttcctcttcc ccccaaaacc caaggacacc      60

ctcatgatct cccggacccc tgaggtcaca tgcgtggtgg tggacgtgag ccacgaagac     120

cctgaggtca agttcaactg gtacgtggac ggcgtggagg tgcataatgc caagacaaag     180

ccgcgggagg agcagtacaa cagcacgtac cgtgtggtca gcgtcctcac cgtcctgcac     240

caggactggc tgaatggcaa ggagtacaag tgcaaggtct ccaacaaagc cctcccagcc     300

cccatcgaga aaaccatctc caaagccaaa gggcagcccc gagaaccaca ggtgtacacc     360

ctgcccccat cccgggagga gatgaccaag aaccaggtca gcctgacctg cctggtcaaa     420

ggcttctatc ccagcgacat cgccgtggag tgggagagca atgggcagcc ggagaacaac     480

tacaagacca cgcctcccgt gctggactcc gacggctcct tcttcctcta cagcaagctc     540

accgtggaca agagcaggtg gcagcagggg aacgtcttct catgctccgt gatgcatgag     600

gctctgcaca accactacac gcagaagagc ctctccctgt ctccgggtaa atga           654


<210> 5
<211> 5
<212> PRT
<213> artificial sequences

<220> 
<221> SOURCE
<222> 1..5
<223> /mol_type="protein"
      /note="Non-structuring sequence or spacer chain"
      /organism="artificial sequences"

<400> 5
Gly Gly Gly Gly Ser 
1               5

<210> 6
<211> 43
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..43
<223> /mol_type="DNA"
      /note="Non-structuring peptide sequence or spacer chain"
      /organism="artificial sequences"

<400> 6
cttggtggag gcggttcagg cggaggtggc tctggcggtg gcg                       43


<210> 7
<211> 436
<212> PRT
<213> artificial sequences

<220> 
<221> SOURCE
<222> 1..436
<223> /mol_type="protein"
      /note="secreted monomeric SAP-Fc "
      /organism="artificial sequences"

<400> 7
His Thr Asp Leu Ser Gly Lys Val Phe Val Phe Pro Arg Glu Ser Val 
1               5                   10                   15    
Thr Asp His Val Asn Leu Ile Thr Pro Leu Glu Lys Pro Leu Gln Asn 
            20                   25                  30        
Phe Thr Leu Cys Phe Arg Ala Tyr Ser Asp Leu Ser Arg Ala Tyr Ser 
        35                   40                  45            
Leu Phe Ser Tyr Asn Thr Gln Gly Arg Asp Asn Glu Leu Leu Val Tyr 
    50                   55                  60                
Lys Glu Arg Val Gly Glu Tyr Ser Leu Tyr Ile Gly Arg His Lys Val 
65                   70                  75                  80
Thr Ser Lys Val Ile Glu Lys Phe Pro Ala Pro Val His Ile Cys Val 
                85                   90                  95    
Ser Trp Glu Ser Ser Ser Gly Ile Ala Glu Phe Trp Ile Asn Gly Thr 
            100                  105                110        
Pro Leu Val Lys Lys Gly Leu Arg Gln Gly Tyr Phe Val Glu Ala Gln 
        115                  120                125            
Pro Lys Ile Val Leu Gly Gln Glu Gln Asp Ser Tyr Gly Gly Lys Phe 
    130                  135                140                
Asp Arg Ser Gln Ser Phe Val Gly Glu Ile Gly Asp Leu Tyr Met Trp 
145                  150                155                  160
Asp Ser Val Leu Pro Pro Glu Asn Ile Leu Ser Ala Tyr Gln Gly Thr 
                165                  170                175    
Pro Leu Pro Ala Asn Ile Leu Asp Trp Gln Ala Leu Asn Tyr Glu Ile 
            180                  185                190        
Arg Gly Tyr Val Ile Ile Lys Pro Leu Val Trp Val Glu Pro Lys Ser 
        195                  200                205            
Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu 
    210                  215                220                
Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
225                  230                235                  240
Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
                245                  250                255    
His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu 
            260                  265                270        
Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr 
        275                  280                285            
Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 
    290                  295                300                
Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro 
305                  310                315                  320
Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln 
                325                  330                335    
Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val 
            340                  345                350        
Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
        355                  360                365            
Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
    370                  375                380                
Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 
385                  390                395                  400
Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
                405                  410                415    
Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
            420                  425                430        
Ser Pro Gly Lys 
        435    

<210> 8
<211> 1311
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..1311
<223> /mol_type="DNA"
      /note="secreted monomeric SAP-Fc "
      /organism="artificial sequences"

<400> 8
cacacagacc tcagtgggaa ggtgtttgta tttcctagag aatctgttac tgatcatgta      60

aacttgatca caccgctgga gaagcctcta cagaacttta ccttgtgttt tcgagcctat     120

agtgatctct ctcgtgccta cagcctcttc tcctacaata cccaaggcag ggataatgag     180

ctactagttt ataaagaaag agttggagag tatagtctat acattggaag acacaaagtt     240

acatccaaag ttatcgaaaa gttcccggct ccagtgcaca tctgtgtgag ctgggagtcc     300

tcatcaggta ttgctgaatt ttggatcaat gggacacctt tggtgaaaaa gggtctgcga     360

cagggttact ttgtggaagc tcagcccaag attgtcctgg ggcaggaaca ggattcctat     420

gggggcaagt ttgataggag ccagtccttt gtgggagaga ttggggattt gtacatgtgg     480

gactctgtgc tgcccccaga aaatatcctg tctgcctatc agggtacccc tctccctgcc     540

aatatcctgg actggcaggc tctgaactat gaaatcagag gatatgtcat catcaaaccc     600

ttggtgtggg tcgagcccaa atcttgtgac aaaactcaca catgcccacc gtgcccagca     660

cctgaactcc tggggggacc gtcagtcttc ctcttccccc caaaacccaa ggacaccctc     720

atgatctccc ggacccctga ggtcacatgc gtggtggtgg acgtgagcca cgaagaccct     780

gaggtcaagt tcaactggta cgtggacggc gtggaggtgc ataatgccaa gacaaagccg     840

cgggaggagc agtacaacag cacgtaccgt gtggtcagcg tcctcaccgt cctgcaccag     900

gactggctga atggcaagga gtacaagtgc aaggtctcca acaaagccct cccagccccc     960

atcgagaaaa ccatctccaa agccaaaggg cagccccgag aaccacaggt gtacaccctg    1020

cccccatccc gggaggagat gaccaagaac caggtcagcc tgacctgcct ggtcaaaggc    1080

ttctatccca gcgacatcgc cgtggagtgg gagagcaatg ggcagccgga gaacaactac    1140

aagaccacgc ctcccgtgct ggactccgac ggctccttct tcctctacag caagctcacc    1200

gtggacaaga gcaggtggca gcaggggaac gtcttctcat gctccgtgat gcatgaggct    1260

ctgcacaacc actacacgca gaagagcctc tccctgtctc cgggtaaatg a             1311


<210> 9
<211> 684
<212> PRT
<213> artificial sequences

<220> 
<221> SOURCE
<222> 1..684
<223> /mol_type="protein"
      /note="Secreted monomeric SAP-ScFc"
      /organism="artificial sequences"

<400> 9
His Thr Asp Leu Ser Gly Lys Val Phe Val Phe Pro Arg Glu Ser Val 
1               5                   10                   15    
Thr Asp His Val Asn Leu Ile Thr Pro Leu Glu Lys Pro Leu Gln Asn 
            20                   25                  30        
Phe Thr Leu Cys Phe Arg Ala Tyr Ser Asp Leu Ser Arg Ala Tyr Ser 
        35                   40                  45            
Leu Phe Ser Tyr Asn Thr Gln Gly Arg Asp Asn Glu Leu Leu Val Tyr 
    50                   55                  60                
Lys Glu Arg Val Gly Glu Tyr Ser Leu Tyr Ile Gly Arg His Lys Val 
65                   70                  75                  80
Thr Ser Lys Val Ile Glu Lys Phe Pro Ala Pro Val His Ile Cys Val 
                85                   90                  95    
Ser Trp Glu Ser Ser Ser Gly Ile Ala Glu Phe Trp Ile Asn Gly Thr 
            100                  105                110        
Pro Leu Val Lys Lys Gly Leu Arg Gln Gly Tyr Phe Val Glu Ala Gln 
        115                  120                125            
Pro Lys Ile Val Leu Gly Gln Glu Gln Asp Ser Tyr Gly Gly Lys Phe 
    130                  135                140                
Asp Arg Ser Gln Ser Phe Val Gly Glu Ile Gly Asp Leu Tyr Met Trp 
145                  150                155                  160
Asp Ser Val Leu Pro Pro Glu Asn Ile Leu Ser Ala Tyr Gln Gly Thr 
                165                  170                175    
Pro Leu Pro Ala Asn Ile Leu Asp Trp Gln Ala Leu Asn Tyr Glu Ile 
            180                  185                190        
Arg Gly Tyr Val Ile Ile Lys Pro Leu Val Trp Val Glu Pro Lys Ser 
        195                  200                205            
Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu 
    210                  215                220                
Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
225                  230                235                  240
Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
                245                  250                255    
His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu 
            260                  265                270        
Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr 
        275                  280                285            
Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 
    290                  295                300                
Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro 
305                  310                315                  320
Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln 
                325                  330                335    
Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val 
            340                  345                350        
Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
        355                  360                365            
Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
    370                  375                380                
Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 
385                  390                395                  400
Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
                405                  410                415    
Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
            420                  425                430        
Ser Pro Gly Lys Leu Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
        435                  440                445            
Gly Gly Gly Ser Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro 
    450                  455                460                
Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe 
465                  470                475                  480
Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val 
                485                  490                495    
Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe 
            500                  505                510        
Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro 
        515                  520                525            
Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr 
    530                  535                540                
Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val 
545                  550                555                  560
Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala 
                565                  570                575    
Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg 
            580                  585                590        
Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly 
        595                  600                605            
Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro 
    610                  615                620                
Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser 
625                  630                635                  640
Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln 
                645                  650                655    
Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His 
            660                  665                670        
Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        675                  680                

<210> 10
<211> 2055
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..2055
<223> /mol_type="DNA"
      /note="Secreted monomeric SAP-ScFc"
      /organism="artificial sequences"

<400> 10
cacacagacc tcagtgggaa ggtgtttgta tttcctagag aatctgttac tgatcatgta      60

aacttgatca caccgctgga gaagcctcta cagaacttta ccttgtgttt tcgagcctat     120

agtgatctct ctcgtgccta cagcctcttc tcctacaata cccaaggcag ggataatgag     180

ctactagttt ataaagaaag agttggagag tatagtctat acattggaag acacaaagtt     240

acatccaaag ttatcgaaaa gttcccggct ccagtgcaca tctgtgtgag ctgggagtcc     300

tcatcaggta ttgctgaatt ttggatcaat gggacacctt tggtgaaaaa gggtctgcga     360

cagggttact ttgtggaagc tcagcccaag attgtcctgg ggcaggaaca ggattcctat     420

gggggcaagt ttgataggag ccagtccttt gtgggagaga ttggggattt gtacatgtgg     480

gactctgtgc tgcccccaga aaatatcctg tctgcctatc agggtacccc tctccctgcc     540

aatatcctgg actggcaggc tctgaactat gaaatcagag gatatgtcat catcaaaccc     600

ttggtgtggg tcgagcccaa atcttgtgac aaaactcaca catgcccacc gtgcccagca     660

cctgaactcc tggggggacc gtcagtcttc ctcttccccc caaaacccaa ggacaccctc     720

atgatctccc ggacccctga ggtcacatgc gtggtggtgg acgtgagcca cgaagaccct     780

gaggtcaagt tcaactggta cgtggacggc gtggaggtgc ataatgccaa gacaaagccg     840

cgggaggagc agtacaacag cacgtaccgt gtggtcagcg tcctcaccgt cctgcaccag     900

gactggctga atggcaagga gtacaagtgc aaggtctcca acaaagccct cccagccccc     960

atcgagaaaa ccatctccaa agccaaaggg cagccccgag aaccacaggt gtacaccctg    1020

cccccatccc gggaggagat gaccaagaac caggtcagcc tgacctgcct ggtcaaaggc    1080

ttctatccca gcgacatcgc cgtggagtgg gagagcaatg ggcagccgga gaacaactac    1140

aagaccacgc ctcccgtgct ggactccgac ggctccttct tcctctatag caagctcacc    1200

gtggacaaga gcaggtggca gcaggggaac gtcttctcat gctccgtgat gcatgaggct    1260

ctgcacaacc actacacgca gaagagcctc tccctgtctc cgggtaagct tggtggaggc    1320

ggttcaggcg gaggtggctc tggcggtggc ggatctgagc ccaaatcttg tgacaaaact    1380

cacacatgcc caccgtgccc agcacctgaa ctcctggggg gaccgtcagt cttcctcttc    1440

cccccaaaac ccaaggacac cctcatgatc tcccggaccc ctgaggtcac atgcgtggtg    1500

gtggacgtga gccacgaaga ccctgaggtc aagttcaact ggtacgtgga cggcgtggag    1560

gtgcataatg ccaagacaaa gccgcgggag gagcagtaca acagcacgta ccgtgtggtc    1620

agcgtcctca ccgtcctgca ccaggactgg ctgaatggca aggagtacaa gtgcaaggtc    1680

tccaacaaag ccctcccagc ccccatcgag aaaaccatct ccaaagccaa agggcagccc    1740

cgagaaccac aggtgtacac cctgccccca tcccgggagg agatgaccaa gaaccaggtc    1800

agcctgacct gcctggtcaa aggcttctat cccagcgaca tcgccgtgga gtgggagagc    1860

aatgggcagc cggagaacaa ctacaagacc acgcctcccg tgctggactc cgacggctcc    1920

ttcttcctct acagcaagct caccgtggac aagagcaggt ggcagcaggg gaacgtcttc    1980

tcatgctccg tgatgcatga ggctctgcac aaccactaca cgcagaagag cctctccctg    2040

tctccgggta aatga                                                     2055


<210> 11
<211> 480
<212> PRT
<213> artificial sequences

<220> 
<221> SOURCE
<222> 1..480
<223> /mol_type="protein"
      /note="Hinge + two fragments of Fc region of human antibody of SA
      P-ScFc"
      /organism="artificial sequences"

<400> 11
Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                   15    
Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                   25                  30        
Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        35                   40                  45            
Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                   55                  60                
Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                   70                  75                  80
Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                   90                  95    
Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                  105                110        
Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                  120                125            
Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr 
    130                  135                140                
Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                  150                155                  160
Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                  170                175    
Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                  185                190        
Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                  200                205            
Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                  215                220                
Ser Leu Ser Leu Ser Pro Gly Lys Leu Gly Gly Gly Gly Ser Gly Gly 
225                  230                235                  240
Gly Gly Ser Gly Gly Gly Gly Ser Glu Pro Lys Ser Cys Asp Lys Thr 
                245                  250                255    
His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser 
            260                  265                270        
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 
        275                  280                285            
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 
    290                  295                300                
Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
305                  310                315                  320
Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val 
                325                  330                335    
Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
            340                  345                350        
Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 
        355                  360                365            
Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
    370                  375                380                
Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys 
385                  390                395                  400
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
                405                  410                415    
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 
            420                  425                430        
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 
        435                  440                445            
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
    450                  455                460                
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
465                  470                475                  480

<210> 12
<211> 1443
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..1443
<223> /mol_type="DNA"
      /note="Hinge + two fragments of Fc region of human antibody of SA
      P-ScFc"
      /organism="artificial sequences"

<400> 12
gagcccaaat cttgtgacaa aactcacaca tgcccaccgt gcccagcacc tgaactcctg      60

gggggaccgt cagtcttcct cttcccccca aaacccaagg acaccctcat gatctcccgg     120

acccctgagg tcacatgcgt ggtggtggac gtgagccacg aagaccctga ggtcaagttc     180

aactggtacg tggacggcgt ggaggtgcat aatgccaaga caaagccgcg ggaggagcag     240

tacaacagca cgtaccgtgt ggtcagcgtc ctcaccgtcc tgcaccagga ctggctgaat     300

ggcaaggagt acaagtgcaa ggtctccaac aaagccctcc cagcccccat cgagaaaacc     360

atctccaaag ccaaagggca gccccgagaa ccacaggtgt acaccctgcc cccatcccgg     420

gaggagatga ccaagaacca ggtcagcctg acctgcctgg tcaaaggctt ctatcccagc     480

gacatcgccg tggagtggga gagcaatggg cagccggaga acaactacaa gaccacgcct     540

cccgtgctgg actccgacgg ctccttcttc ctctatagca agctcaccgt ggacaagagc     600

aggtggcagc aggggaacgt cttctcatgc tccgtgatgc atgaggctct gcacaaccac     660

tacacgcaga agagcctctc cctgtctccg ggtaagcttg gtggaggcgg ttcaggcgga     720

ggtggctctg gcggtggcgg atctgagccc aaatcttgtg acaaaactca cacatgccca     780

ccgtgcccag cacctgaact cctgggggga ccgtcagtct tcctcttccc cccaaaaccc     840

aaggacaccc tcatgatctc ccggacccct gaggtcacat gcgtggtggt ggacgtgagc     900

cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt gcataatgcc     960

aagacaaagc cgcgggagga gcagtacaac agcacgtacc gtgtggtcag cgtcctcacc    1020

gtcctgcacc aggactggct gaatggcaag gagtacaagt gcaaggtctc caacaaagcc    1080

ctcccagccc ccatcgagaa aaccatctcc aaagccaaag ggcagccccg agaaccacag    1140

gtgtacaccc tgcccccatc ccgggaggag atgaccaaga accaggtcag cctgacctgc    1200

ctggtcaaag gcttctatcc cagcgacatc gccgtggagt gggagagcaa tgggcagccg    1260

gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt cttcctctac    1320

agcaagctca ccgtggacaa gagcaggtgg cagcagggga acgtcttctc atgctccgtg    1380

atgcatgagg ctctgcacaa ccactacacg cagaagagcc tctccctgtc tccgggtaaa    1440

tga                                                                  1443


<210> 13
<211> 15
<212> PRT
<213> Homo sapiens

<220> 
<221> SOURCE
<222> 1..15
<223> /mol_type="protein"
      /note="Hinge region of human IgG1"
      /organism="Homo sapiens"

<400> 13
Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                   15

<210> 14
<211> 23
<212> DNA
<213> Homo sapiens

<220> 
<221> source
<222> 1..23
<223> /mol_type="DNA"
      /note="Hinge region of human IgG1"
      /organism="Homo sapiens"

<400> 14
ctcacacatg cccaccgtgc cca                                            23


<210> 15
<211> 12
<212> PRT
<213> Homo sapiens

<220> 
<221> SOURCE
<222> 1..12
<223> /mol_type="protein"
      /note="Hinge region of human IgG2"
      /organism="Homo sapiens"

<400> 15
Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro 
1               5                   10        

<210> 16
<211> 17
<212> PRT
<213> Homo sapiens

<220> 
<221> SOURCE
<222> 1..17
<223> /mol_type="protein"
      /note="Hinge region of human IgG3"
      /organism="Homo sapiens"

<400> 16
Glu Leu Lys Thr Pro Leu Gly Asp Thr Thr His Thr Cys Pro Arg Cys 
1               5                   10                   15    
Pro 
    

<210> 17
<211> 15
<212> PRT
<213> Homo sapiens

<220> 
<221> SOURCE
<222> 1..15
<223> /mol_type="protein"
      /note="Hinge region of human IgG3"
      /organism="Homo sapiens"

<400> 17
Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro 
1               5                   10                   15

<210> 18
<211> 12
<212> PRT
<213> Homo sapiens

<220> 
<221> SOURCE
<222> 1..12
<223> /mol_type="protein"
      /note="Hinge region of human IgG4"
      /organism="Homo sapiens"

<400> 18
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro 
1               5                   10        

<210> 19
<211> 10
<212> PRT
<213> Homo sapiens

<220> 
<221> SOURCE
<222> 1..10
<223> /mol_type="protein"
      /note="Shortened hinge region of IgG1 "
      /organism="Homo sapiens"

<400> 19
Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10

<210> 20
<211> 19
<212> PRT
<213> Homo sapiens

<220> 
<221> SOURCE
<222> 1..19
<223> /mol_type="protein"
      /note="Natural signal peptide of SAP"
      /organism="Homo sapiens"

<400> 20
Met Asn Lys Pro Leu Leu Trp Ile Ser Val Leu Thr Ser Leu Leu Glu 
1               5                   10                   15    
Ala Phe Ala 
            

<210> 21
<211> 57
<212> DNA
<213> Homo sapiens

<220> 
<221> source
<222> 1..57
<223> /mol_type="DNA"
      /note="Natural signal peptide of SAP"
      /organism="Homo sapiens"

<400> 21
atgaacaagc cgctcctttg gatctctgtc ctcaccagcc tcctggaagc ctttgct        57


<210> 22
<211> 23
<212> PRT
<213> Homo sapiens

<220> 
<221> SOURCE
<222> 1..23
<223> /mol_type="protein"
      /note="Human TIMP signal peptide MMP1"
      /organism="Homo sapiens"

<400> 22
Met Ala Pro Phe Glu Pro Leu Ala Ser Gly Ile Leu Leu Leu Leu Trp 
1               5                   10                   15    
Leu Ile Ala Pro Ser Arg Ala 
            20            

<210> 23
<211> 24
<212> PRT
<213> Homo sapiens

<220> 
<221> SOURCE
<222> 1..24
<223> /mol_type="protein"
      /note="human insulin signal peptide"
      /organism="Homo sapiens"

<400> 23
Met Ala Leu Trp Met Arg Leu Leu Pro Leu Leu Ala Leu Leu Ala Leu 
1               5                   10                   15    
Trp Gly Pro Asp Pro Ala Ala Ala 
            20                

<210> 24
<211> 27
<212> PRT
<213> Homo sapiens

<220> 
<221> SOURCE
<222> 1..27
<223> /mol_type="protein"
      /note="human EPO signal peptide"
      /organism="Homo sapiens"

<400> 24
Met Gly Val His Glu Cys Pro Ala Trp Leu Trp Leu Leu Leu Ser Leu 
1               5                   10                   15    
Leu Ser Leu Pro Leu Gly Leu Pro Val Leu Gly 
            20                   25        

<210> 25
<211> 18
<212> PRT
<213> artificial sequences

<220> 
<221> SOURCE
<222> 1..18
<223> /mol_type="protein"
      /note="MB7 signal peptide "
      /organism="artificial sequences"

<400> 25
Met Arg Trp Ser Trp Ile Phe Leu Leu Leu Leu Ser Ile Thr Ser Ala 
1               5                   10                   15    
Asn Ala 
        

<210> 26
<211> 19
<212> PRT
<213> artificial sequences

<220> 
<221> SOURCE
<222> 1..19
<223> /mol_type="protein"
      /note="AMHRII signal peptide "
      /organism="artificial sequences"

<400> 26
Met Arg Trp Ser Trp Ile Phe Leu Phe Leu Leu Ser Ile Thr Ala Ser 
1               5                   10                   15    
Val His Cys 
            

<210> 27
<211> 19
<212> PRT
<213> artificial sequences

<220> 
<221> SOURCE
<222> 1..19
<223> /mol_type="protein"
      /note="XXII49 signal peptide "
      /organism="artificial sequences"

<400> 27
Met Ala Trp Val Trp Thr Leu Leu Phe Leu Met Ala Ala Ala Gln Ser 
1               5                   10                   15    
Ala Gln Ala 
            

<210> 28
<211> 680
<212> DNA
<213> artificial sequences

<220> 
<221> SOURCE
<222> 1..680
<223> /mol_type="DNA"
      /note="SAP"
      /organism="artificial sequences"

<400> 28
gtcgacacca tgaacaagcc gctctttgga tctctgtcct caccagcctc ctggaagcct      60

ttgctcacac agacctcagt gggaaggtgt ttgtatttcc tagagaatct gttactgatc     120

atgtaaactt gatcacaccg ctggagaagc ctctacagaa ctttaccttg tgttttcgag     180

cctatagtga tctctctcgt gcctacagcc tcttctccta caatacccaa ggcagggata     240

atgagctact agtttataaa gaaagagttg gagagtatag tctatacatt ggaagacaca     300

aagttacatc caaagttatc gaaaagttcc cggctccagt gcacatctgt gtgagctggg     360

agtcctcatc aggtattgct gaattttgga tcaatgggac acctttggtg aaaaagggtc     420

tgcgacaggg ttactttgtg gaagctcagc ccaagattgt cctggggcag gaacaggatt     480

cctatggggg caagtttgat aggagccagt cctttgtggg agagattggg gatttgtaca     540

tgtgggactc tgtgctgccc ccagaaaata tcctgtctgc ctatcagggt acccctctcc     600

ctgccaatat cctggactgg caggctctga actatgaaat cagaggatat gtcatcatca     660

aacccttggt gtgggtcgac                                                 680


<210> 29
<211> 722
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..722
<223> /mol_type="DNA"
      /note="Fragment of the Fc region of a human antibody"
      /organism="artificial sequences"

<400> 29
atactctcga gcccaaatct tgtgacaaaa ctcacacatg cccaccgtgc ccagcacctg      60

aactcctggg gggaccgtca gtcttcctct tccccccaaa acccaaggac accctcatga     120

tctcccggac ccctgaggtc acatgcgtgg tggtggacgt gagccacgaa gaccctgagg     180

tcaagttcaa ctggtacgtg gacggcgtgg aggtgcataa tgccaagaca aagccgcggg     240

aggagcagta caacagcacg taccgtgtgg tcagcgtcct caccgtcctg caccaggact     300

ggctgaatgg caaggagtac aagtgcaagg tctccaacaa agccctccca gcccccatcg     360

agaaaaccat ctccaaagcc aaagggcagc cccgagaacc acaggtgtac accctgcccc     420

catcccggga ggagatgacc aagaaccagg tcagcctgac ctgcctggtc aaaggcttct     480

atcccagcga catcgccgtg gagtgggaga gcaatgggca gccggagaac aactacaaga     540

ccacgcctcc cgtgctggac tccgacggct ccttcttcct ctacagcaag ctcaccgtgg     600

acaagagcag gtggcagcag gggaacgtct tctcatgctc cgtgatgcat gaggctctgc     660

acaaccacta cacgcagaag agcctctccc tgtctccggg taaatgagtg ctccggacag     720

at                                                                    722


<210> 30
<211> 1368
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..1368
<223> /mol_type="DNA"
      /note="Monomeric SAP-Fc"
      /organism="artificial sequences"

<400> 30
atgaacaagc cgctcctttg gatctctgtc ctcaccagcc tcctggaagc ctttgctcac      60

acagacctca gtgggaaggt gtttgtattt cctagagaat ctgttactga tcatgtaaac     120

ttgatcacac cgctggagaa gcctctacag aactttacct tgtgttttcg agcctatagt     180

gatctctctc gtgcctacag cctcttctcc tacaataccc aaggcaggga taatgagcta     240

ctagtttata aagaaagagt tggagagtat agtctataca ttggaagaca caaagttaca     300

tccaaagtta tcgaaaagtt cccggctcca gtgcacatct gtgtgagctg ggagtcctca     360

tcaggtattg ctgaattttg gatcaatggg acacctttgg tgaaaaaggg tctgcgacag     420

ggttactttg tggaagctca gcccaagatt gtcctggggc aggaacagga ttcctatggg     480

ggcaagtttg ataggagcca gtcctttgtg ggagagattg gggatttgta catgtgggac     540

tctgtgctgc ccccagaaaa tatcctgtct gcctatcagg gtacccctct ccctgccaat     600

atcctggact ggcaggctct gaactatgaa atcagaggat atgtcatcat caaacccttg     660

gtgtgggtcg agcccaaatc ttgtgacaaa actcacacat gcccaccgtg cccagcacct     720

gaactcctgg ggggaccgtc agtcttcctc ttccccccaa aacccaagga caccctcatg     780

atctcccgga cccctgaggt cacatgcgtg gtggtggacg tgagccacga agaccctgag     840

gtcaagttca actggtacgt ggacggcgtg gaggtgcata atgccaagac aaagccgcgg     900

gaggagcagt acaacagcac gtaccgtgtg gtcagcgtcc tcaccgtcct gcaccaggac     960

tggctgaatg gcaaggagta caagtgcaag gtctccaaca aagccctccc agcccccatc    1020

gagaaaacca tctccaaagc caaagggcag ccccgagaac cacaggtgta caccctgccc    1080

ccatcccggg aggagatgac caagaaccag gtcagcctga cctgcctggt caaaggcttc    1140

tatcccagcg acatcgccgt ggagtgggag agcaatgggc agccggagaa caactacaag    1200

accacgcctc ccgtgctgga ctccgacggc tccttcttcc tctacagcaa gctcaccgtg    1260

gacaagagca ggtggcagca ggggaacgtc ttctcatgct ccgtgatgca tgaggctctg    1320

cacaaccact acacgcagaa gagcctctcc ctgtctccgg gtaaatga                 1368


<210> 31
<211> 455
<212> PRT
<213> artificial sequences

<220> 
<221> SOURCE
<222> 1..455
<223> /mol_type="protein"
      /note="Monomeric SAP-Fc"
      /organism="artificial sequences"

<400> 31
Met Asn Lys Pro Leu Leu Trp Ile Ser Val Leu Thr Ser Leu Leu Glu 
1               5                   10                   15    
Ala Phe Ala His Thr Asp Leu Ser Gly Lys Val Phe Val Phe Pro Arg 
            20                   25                  30        
Glu Ser Val Thr Asp His Val Asn Leu Ile Thr Pro Leu Glu Lys Pro 
        35                   40                  45            
Leu Gln Asn Phe Thr Leu Cys Phe Arg Ala Tyr Ser Asp Leu Ser Arg 
    50                   55                  60                
Ala Tyr Ser Leu Phe Ser Tyr Asn Thr Gln Gly Arg Asp Asn Glu Leu 
65                   70                  75                  80
Leu Val Tyr Lys Glu Arg Val Gly Glu Tyr Ser Leu Tyr Ile Gly Arg 
                85                   90                  95    
His Lys Val Thr Ser Lys Val Ile Glu Lys Phe Pro Ala Pro Val His 
            100                  105                110        
Ile Cys Val Ser Trp Glu Ser Ser Ser Gly Ile Ala Glu Phe Trp Ile 
        115                  120                125            
Asn Gly Thr Pro Leu Val Lys Lys Gly Leu Arg Gln Gly Tyr Phe Val 
    130                  135                140                
Glu Ala Gln Pro Lys Ile Val Leu Gly Gln Glu Gln Asp Ser Tyr Gly 
145                  150                155                  160
Gly Lys Phe Asp Arg Ser Gln Ser Phe Val Gly Glu Ile Gly Asp Leu 
                165                  170                175    
Tyr Met Trp Asp Ser Val Leu Pro Pro Glu Asn Ile Leu Ser Ala Tyr 
            180                  185                190        
Gln Gly Thr Pro Leu Pro Ala Asn Ile Leu Asp Trp Gln Ala Leu Asn 
        195                  200                205            
Tyr Glu Ile Arg Gly Tyr Val Ile Ile Lys Pro Leu Val Trp Val Glu 
    210                  215                220                
Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro 
225                  230                235                  240
Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
                245                  250                255    
Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
            260                  265                270        
Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
        275                  280                285            
Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
    290                  295                300                
Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
305                  310                315                  320
Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
                325                  330                335    
Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
            340                  345                350        
Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys 
        355                  360                365            
Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
    370                  375                380                
Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
385                  390                395                  400
Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
                405                  410                415    
Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
            420                  425                430        
Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
        435                  440                445            
Leu Ser Leu Ser Pro Gly Lys 
    450                  455

<210> 32
<211> 654
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..654
<223> /mol_type="DNA"
      /note="First fragment of Fc region of a human antibody"
      /organism="artificial sequences"

<400> 32
gcacctgaac tcctgggggg accgtcagtc ttcctcttcc ccccaaaacc caaggacacc      60

ctcatgatct cccggacccc tgaggtcaca tgcgtggtgg tggacgtgag ccacgaagac     120

cctgaggtca agttcaactg gtacgtggac ggcgtggagg tgcataatgc caagacaaag     180

ccgcgggagg agcagtacaa cagcacgtac cgtgtggtca gcgtcctcac cgtcctgcac     240

caggactggc tgaatggcaa ggagtacaag tgcaaggtct ccaacaaagc cctcccagcc     300

cccatcgaga aaaccatctc caaagccaaa gggcagcccc gagaaccaca ggtgtacacc     360

ctgcccccat cccgggagga gatgaccaag aaccaggtca gcctgacctg cctggtcaaa     420

ggcttctatc ccagcgacat cgccgtggag tgggagagca atgggcagcc ggagaacaac     480

tacaagacca cgcctcccgt gctggactcc gacggctcct tcttcctcta tagcaagctc     540

accgtggaca agagcaggtg gcagcagggg aacgtcttct catgctccgt gatgcatgag     600

gctctgcaca accactacac gcagaagagc ctctccctgt ctccgggtaa gctt           654


<210> 33
<211> 715
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..715
<223> /mol_type="DNA"
      /note="Second fragment of Fc region of a human antibody"
      /organism="artificial sequences"

<400> 33
agatctgagc ccaaatcttg tgacaaaact cacacatgcc caccgtgccc agcacctgaa      60

ctcctggggg gaccgtcagt cttcctcttc cccccaaaac ccaaggacac cctcatgatc     120

tcccggaccc ctgaggtcac atgcgtggtg gtggacgtga gccacgaaga ccctgaggtc     180

aagttcaact ggtacgtgga cggcgtggag gtgcataatg ccaagacaaa gccgcgggag     240

gagcagtaca acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca ccaggactgg     300

ctgaatggca aggagtacaa gtgcaaggtc tccaacaaag ccctcccagc ccccatcgag     360

aaaaccatct ccaaagccaa agggcagccc cgagaaccac aggtgtacac cctgccccca     420

tcccgggagg agatgaccaa gaaccaggtc agcctgacct gcctggtcaa aggcttctat     480

cccagcgaca tcgccgtgga gtgggagagc aatgggcagc cggagaacaa ctacaagacc     540

acgcctcccg tgctggactc cgacggctcc ttcttcctct acagcaagct caccgtggac     600

aagagcaggt ggcagcaggg gaacgtcttc tcatgctccg tgatgcatga ggctctgcac     660

aaccactaca cgcagaagag cctctccctg tctccgggta aatgagtgct ccgga          715


<210> 34
<211> 51
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..51
<223> /mol_type="DNA"
      /note="spacer chain"
      /organism="artificial sequences"

<400> 34
aagcttggtg gaggcggttc aggcggaggt ggctctggcg gtggcggatc c              51


<210> 35
<211> 2112
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..2112
<223> /mol_type="DNA"
      /note="Monomeric SAP-ScFc"
      /organism="artificial sequences"

<400> 35
atgaacaagc cgctcctttg gatctctgtc ctcaccagcc tcctggaagc ctttgctcac      60

acagacctca gtgggaaggt gtttgtattt cctagagaat ctgttactga tcatgtaaac     120

ttgatcacac cgctggagaa gcctctacag aactttacct tgtgttttcg agcctatagt     180

gatctctctc gtgcctacag cctcttctcc tacaataccc aaggcaggga taatgagcta     240

ctagtttata aagaaagagt tggagagtat agtctataca ttggaagaca caaagttaca     300

tccaaagtta tcgaaaagtt cccggctcca gtgcacatct gtgtgagctg ggagtcctca     360

tcaggtattg ctgaattttg gatcaatggg acacctttgg tgaaaaaggg tctgcgacag     420

ggttactttg tggaagctca gcccaagatt gtcctggggc aggaacagga ttcctatggg     480

ggcaagtttg ataggagcca gtcctttgtg ggagagattg gggatttgta catgtgggac     540

tctgtgctgc ccccagaaaa tatcctgtct gcctatcagg gtacccctct ccctgccaat     600

atcctggact ggcaggctct gaactatgaa atcagaggat atgtcatcat caaacccttg     660

gtgtgggtcg agcccaaatc ttgtgacaaa actcacacat gcccaccgtg cccagcacct     720

gaactcctgg ggggaccgtc agtcttcctc ttccccccaa aacccaagga caccctcatg     780

atctcccgga cccctgaggt cacatgcgtg gtggtggacg tgagccacga agaccctgag     840

gtcaagttca actggtacgt ggacggcgtg gaggtgcata atgccaagac aaagccgcgg     900

gaggagcagt acaacagcac gtaccgtgtg gtcagcgtcc tcaccgtcct gcaccaggac     960

tggctgaatg gcaaggagta caagtgcaag gtctccaaca aagccctccc agcccccatc    1020

gagaaaacca tctccaaagc caaagggcag ccccgagaac cacaggtgta caccctgccc    1080

ccatcccggg aggagatgac caagaaccag gtcagcctga cctgcctggt caaaggcttc    1140

tatcccagcg acatcgccgt ggagtgggag agcaatgggc agccggagaa caactacaag    1200

accacgcctc ccgtgctgga ctccgacggc tccttcttcc tctatagcaa gctcaccgtg    1260

gacaagagca ggtggcagca ggggaacgtc ttctcatgct ccgtgatgca tgaggctctg    1320

cacaaccact acacgcagaa gagcctctcc ctgtctccgg gtaagcttgg tggaggcggt    1380

tcaggcggag gtggctctgg cggtggcgga tctgagccca aatcttgtga caaaactcac    1440

acatgcccac cgtgcccagc acctgaactc ctggggggac cgtcagtctt cctcttcccc    1500

ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg    1560

gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg    1620

cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc    1680

gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg caaggtctcc    1740

aacaaagccc tcccagcccc catcgagaaa accatctcca aagccaaagg gcagccccga    1800

gaaccacagg tgtacaccct gcccccatcc cgggaggaga tgaccaagaa ccaggtcagc    1860

ctgacctgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg ggagagcaat    1920

gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc    1980

ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa cgtcttctca    2040

tgctccgtga tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct    2100

ccgggtaaat ga                                                        2112


<210> 36
<211> 703
<212> PRT
<213> artificial sequences

<220> 
<221> SOURCE
<222> 1..703
<223> /mol_type="protein"
      /note="Monomeric SAP-ScFc"
      /organism="artificial sequences"

<400> 36
Met Asn Lys Pro Leu Leu Trp Ile Ser Val Leu Thr Ser Leu Leu Glu 
1               5                   10                   15    
Ala Phe Ala His Thr Asp Leu Ser Gly Lys Val Phe Val Phe Pro Arg 
            20                   25                  30        
Glu Ser Val Thr Asp His Val Asn Leu Ile Thr Pro Leu Glu Lys Pro 
        35                   40                  45            
Leu Gln Asn Phe Thr Leu Cys Phe Arg Ala Tyr Ser Asp Leu Ser Arg 
    50                   55                  60                
Ala Tyr Ser Leu Phe Ser Tyr Asn Thr Gln Gly Arg Asp Asn Glu Leu 
65                   70                  75                  80
Leu Val Tyr Lys Glu Arg Val Gly Glu Tyr Ser Leu Tyr Ile Gly Arg 
                85                   90                  95    
His Lys Val Thr Ser Lys Val Ile Glu Lys Phe Pro Ala Pro Val His 
            100                  105                110        
Ile Cys Val Ser Trp Glu Ser Ser Ser Gly Ile Ala Glu Phe Trp Ile 
        115                  120                125            
Asn Gly Thr Pro Leu Val Lys Lys Gly Leu Arg Gln Gly Tyr Phe Val 
    130                  135                140                
Glu Ala Gln Pro Lys Ile Val Leu Gly Gln Glu Gln Asp Ser Tyr Gly 
145                  150                155                  160
Gly Lys Phe Asp Arg Ser Gln Ser Phe Val Gly Glu Ile Gly Asp Leu 
                165                  170                175    
Tyr Met Trp Asp Ser Val Leu Pro Pro Glu Asn Ile Leu Ser Ala Tyr 
            180                  185                190        
Gln Gly Thr Pro Leu Pro Ala Asn Ile Leu Asp Trp Gln Ala Leu Asn 
        195                  200                205            
Tyr Glu Ile Arg Gly Tyr Val Ile Ile Lys Pro Leu Val Trp Val Glu 
    210                  215                220                
Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro 
225                  230                235                  240
Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
                245                  250                255    
Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
            260                  265                270        
Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
        275                  280                285            
Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
    290                  295                300                
Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
305                  310                315                  320
Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
                325                  330                335    
Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
            340                  345                350        
Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys 
        355                  360                365            
Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
    370                  375                380                
Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
385                  390                395                  400
Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
                405                  410                415    
Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
            420                  425                430        
Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
        435                  440                445            
Leu Ser Leu Ser Pro Gly Lys Leu Gly Gly Gly Gly Ser Gly Gly Gly 
    450                  455                460                
Gly Ser Gly Gly Gly Gly Ser Glu Pro Lys Ser Cys Asp Lys Thr His 
465                  470                475                  480
Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val 
                485                  490                495    
Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
            500                  505                510        
Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
        515                  520                525            
Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
    530                  535                540                
Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
545                  550                555                  560
Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
                565                  570                575    
Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
            580                  585                590        
Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
        595                  600                605            
Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
    610                  615                620                
Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
625                  630                635                  640
Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
                645                  650                655    
Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
            660                  665                670        
Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
        675                  680                685            
His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    690                  695                700            

<210> 37
<211> 34
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..34
<223> /mol_type="DNA"
      /note="First primer for the amplification of the SAP "
      /organism="artificial sequences"

<400> 37
acttggtcga caccatgaac aagccgctgc tttg                                 34


<210> 38
<211> 28
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..28
<223> /mol_type="DNA"
      /note="Second primer for the amplification of the SAP"
      /organism="artificial sequences"

<400> 38
actaggtcga cccacaccaa gggtttga                                       28


<210> 39
<211> 23
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..23
<223> /mol_type="DNA"
      /note="First primer for the amplification of the fragment of Fc r
      egion of a human antibody"
      /organism="artificial sequences"

<400> 39
ctcgagccca aatcttgtga caa                                            23


<210> 40
<211> 26
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..26
<223> /mol_type="DNA"
      /note="Second primer for the amplification of the fragment of Fc 
      region of a human antibody"
      /organism="artificial sequences"

<400> 40
tccggagcac tcatttaccc ggagac                                         26


<210> 41
<211> 28
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..28
<223> /mol_type="DNA"
      /note="First primer for the amplification of the first fragment o
      f Fc region of a human antibody"
      /organism="artificial sequences"

<400> 41
atactctcga gcccaaatct tgtgacaa                                       28


<210> 42
<211> 28
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..28
<223> /mol_type="DNA"
      /note="Second primer for the amplification of the first fragment 
      of Fc region of a human antibody"
      /organism="artificial sequences"

<400> 42
atctgaagct tacccggaga cagggaga                                       28


<210> 43
<211> 31
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..31
<223> /mol_type="DNA"
      /note="First primer for the amplification of the second fragment 
      of Fc region of a human antibody"
      /organism="artificial sequences"

<400> 43
aagtaagatc tgagcccaaa tcttgtgaca a                                   31


<210> 44
<211> 31
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..31
<223> /mol_type="DNA"
      /note="Second primer for the amplification of the second fragment
       of Fc region of a human antibody"
      /organism="artificial sequences"

<400> 44
atctgtccgg agcactcatt tacccggaga c                                   31


<210> 45
<211> 23
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..23
<223> /mol_type="DNA"
      /note="First primer for the amplification of the spacer chain"
      /organism="artificial sequences"

<400> 45
aagcttggtg gaggcggttc agg                                            23


<210> 46
<211> 22
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..22
<223> /mol_type="DNA"
      /note="Second primer for the amplification of the spacer chain"
      /organism="artificial sequences"

<400> 46
ggatccgcca ccgccagagc ca                                             22


