               SEQUENCE LISTING

<110> Muster Thomas

<120> NOVEL INFLUENZA VIRUS VECTOR FOR VIROTHERAPY

<130> MT001P

<160> 10

<170> BiSSAP 1.3

<210> 1
<211> 230
<212> PRT
<213> Artificial Sequence

<220> 
<223> wild-type PR8 NS1

<400> 1
Met Asp Pro Asn Thr Val Ser Ser Phe Gln Val Asp Cys Phe Leu Trp 
1               5                   10                  15      
His Val Arg Lys Arg Val Ala Asp Gln Glu Leu Gly Asp Ala Pro Phe 
            20                  25                  30          
Leu Asp Arg Leu Arg Arg Asp Gln Lys Ser Leu Arg Gly Arg Gly Ser 
        35                  40                  45              
Thr Leu Gly Leu Asp Ile Glu Thr Ala Thr Arg Ala Gly Lys Gln Ile 
    50                  55                  60                  
Val Glu Arg Ile Leu Lys Glu Glu Ser Asp Glu Ala Leu Lys Met Thr 
65                  70                  75                  80  
Met Ala Ser Val Pro Ala Ser Arg Tyr Leu Thr Asp Met Thr Leu Glu 
                85                  90                  95      
Glu Met Ser Arg Asp Trp Ser Met Leu Ile Pro Lys Gln Lys Val Ala 
            100                 105                 110         
Gly Pro Leu Cys Ile Arg Met Asp Gln Ala Ile Met Asp Lys Asn Ile 
        115                 120                 125             
Ile Leu Lys Ala Asn Phe Ser Val Ile Phe Asp Arg Leu Glu Thr Leu 
    130                 135                 140                 
Ile Leu Leu Arg Ala Phe Thr Glu Glu Gly Ala Ile Val Gly Glu Ile 
145                 150                 155                 160 
Ser Pro Leu Pro Ser Leu Pro Gly His Thr Ala Glu Asp Val Lys Asn 
                165                 170                 175     
Ala Val Gly Val Leu Ile Gly Gly Leu Glu Trp Asn Asp Asn Thr Val 
            180                 185                 190         
Arg Val Ser Glu Thr Leu Gln Arg Phe Ala Trp Arg Ser Ser Asn Glu 
        195                 200                 205             
Asn Gly Arg Pro Pro Leu Thr Pro Lys Gln Lys Arg Glu Met Ala Gly 
    210                 215                 220                 
Thr Ile Arg Ser Glu Val 
225                 230 

<210> 2
<211> 1259
<212> DNA
<213> Artificial Sequence

<220> 
<223> delNS106-2A-mGM-CSF

<400> 2
agcaaaagca gggtgacaaa gacataatgg atccaaacac tgtgtcaagc tttcaggtag      60

attgctttct ttggcatgtc cgcaaacgag ttgcagacca agaactaggt gatgccccat     120

tccttgatcg gcttcgccga gatcagaaat ccctaagagg aaggggcagc accctcggtc     180

tggacatcga gacagccaca cgtgctggaa agcagatagt ggagcggatt ctgaaagaag     240

aatccgatga ggcacttaaa atgaccatgg cctctgtacc tgcgtcgcgt tacctaactg     300

acatgactct tgaggaaatg tcaagggact ggtccatgct cataggggga aatttcgatc     360

ttctaaaact tgcaggggat gtggaatcaa atccaggacc aatgaagaca gatacacttc     420

ttctttgggt gctgcttttg tgggttccaa gatcacatgg ggcaccaaca agatcaccaa     480

ttacagtgac aagaccatgg aaacacgtgg aagcaatcaa agaagcactt aatctgcttg     540

atgatatgcc agtgacactg aatgaagagg tggaagtggt gtcaaatgag ttcagcttca     600

aaaaactgac atgcgtgcag acaagactga aaattttcga acaaggactg aggggaaact     660

tcacaaaact taaaggggca ctgaatatga cagcaagcta ttatcaaaca tactgcccac     720

caacaccaga aacagattgc gaaacacaag tgacaacata cgcagatttc atcgacagcc     780

ttaaaacatt cctgacagat atcccattcg agtgcaaaaa accagggcag aagtgataat     840

aagcggccgc ccaagcagaa agtggtacta accttcttct ctttcttctc ctgacaggac     900

atactgctga ggatgtcaaa aatgcagttg gagtcctcat cgggggactt gaatggaatg     960

ataacacagt tcgagtctct gaaactctac agagattcgc ttggagaagc agtaatgaga    1020

atgggagacc tccactcact ccaaaacaga aacgagaaat ggcgggaaca attaggtcag    1080

aagtttgaag aaataagatg gttgattgaa gaagtgagac acaaactgaa gataacagag    1140

aatagttttg agcaaataac atttatgcaa gccttacatc tattgcttga agtggagcaa    1200

gagataagaa ctttctcgtt tcagcttatt taataataaa aaacaccctt gtttctact     1259


<210> 3
<211> 269
<212> PRT
<213> Artificial Sequence

<220> 
<223> NS106-2A-mGM-CSF

<400> 3
Met Asp Pro Asn Thr Val Ser Ser Phe Gln Val Asp Cys Phe Leu Trp 
1               5                   10                  15      
His Val Arg Lys Arg Val Ala Asp Gln Glu Leu Gly Asp Ala Pro Phe 
            20                  25                  30          
Leu Asp Arg Leu Arg Arg Asp Gln Lys Ser Leu Arg Gly Arg Gly Ser 
        35                  40                  45              
Thr Leu Gly Leu Asp Ile Glu Thr Ala Thr Arg Ala Gly Lys Gln Ile 
    50                  55                  60                  
Val Glu Arg Ile Leu Lys Glu Glu Ser Asp Glu Ala Leu Lys Met Thr 
65                  70                  75                  80  
Met Ala Ser Val Pro Ala Ser Arg Tyr Leu Thr Asp Met Thr Leu Glu 
                85                  90                  95      
Glu Met Ser Arg Asp Trp Ser Met Leu Ile Gly Gly Asn Phe Asp Leu 
            100                 105                 110         
Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro Met Lys Thr 
        115                 120                 125             
Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro Arg Ser His 
    130                 135                 140                 
Gly Ala Pro Thr Arg Ser Pro Ile Thr Val Thr Arg Pro Trp Lys His 
145                 150                 155                 160 
Val Glu Ala Ile Lys Glu Ala Leu Asn Leu Leu Asp Asp Met Pro Val 
                165                 170                 175     
Thr Leu Asn Glu Glu Val Glu Val Val Ser Asn Glu Phe Ser Phe Lys 
            180                 185                 190         
Lys Leu Thr Cys Val Gln Thr Arg Leu Lys Ile Phe Glu Gln Gly Leu 
        195                 200                 205             
Arg Gly Asn Phe Thr Lys Leu Lys Gly Ala Leu Asn Met Thr Ala Ser 
    210                 215                 220                 
Tyr Tyr Gln Thr Tyr Cys Pro Pro Thr Pro Glu Thr Asp Cys Glu Thr 
225                 230                 235                 240 
Gln Val Thr Thr Tyr Ala Asp Phe Ile Asp Ser Leu Lys Thr Phe Leu 
                245                 250                 255     
Thr Asp Ile Pro Phe Glu Cys Lys Lys Pro Gly Gln Lys 
            260                 265                 

<210> 4
<211> 1544
<212> DNA
<213> Artificial Sequence

<220> 
<223> delNS106-2A-GFP

<400> 4
agcaaaagca gggtgacaaa gacataatgg atccaaacac tgtgtcaagc tttcaggtag      60

attgctttct ttggcatgtc cgcaaacgag ttgcagacca agaactaggt gatgccccat     120

tccttgatcg gcttcgccga gatcagaaat ccctaagagg aaggggcagc accctcggtc     180

tggacatcga gacagccaca cgtgctggaa agcagatagt ggagcggatt ctgaaagaag     240

aatccgatga ggcacttaaa atgaccatgg cctctgtacc tgcgtcgcgt tacctaactg     300

acatgactct tgaggaaatg tcaagggact ggtccatgct cataggggga aatttcgatc     360

ttctaaaact tgcaggggat gtggaatcaa atccaggacc aatggcatca aaaggggaag     420

aactttttac aggggtggtg ccaatacttg tggaacttga tggggatgtg aatggacaca     480

aattctcagt tagcggagag ggagaaggag atgcaacata cggaaaactt acactgaaat     540

tcatctgcac aactggaaaa cttccagttc catggccaac acttgtgaca acactttgtt     600

atggggtgca atgcttctca agatacccag atcatatgaa gaggcacgat ttcttcaaat     660

cagcaatgcc agagggatac gtgcaagaga gaacaatatt cttcaaagac gacgggaact     720

acaagacaag agcagaagtg aaattcgagg gggatacact tgtgaataga atagaactga     780

agggaatcga cttcaaagag gatggaaata ttctgggaca caagctcgag tacaactaca     840

atagccataa tgtgtacatc atggccgaca agcagaaaaa cggaatcaaa gtgaacttca     900

agactaggca taatattgag gatggatcag tgcaactggc agatcattat caacaaaaca     960

caccaattgg agatggacca gtgcttctgc cagataatca ttacctttca acacagtcag    1020

cactgagcaa agatccaaat gagaaaaggg atcatatggt gctgcttgaa tttgtgacag    1080

cagctggaat tacacatgga atggatgagc tgtacaactg ataataagcg gccgcccaag    1140

cagaaagtgg tactaacctt cttctctttc ttctcctgac aggacatact gctgaggatg    1200

tcaaaaatgc agttggagtc ctcatcgggg gacttgaatg gaatgataac acagttcgag    1260

tctctgaaac tctacagaga ttcgcttgga gaagcagtaa tgagaatggg agacctccac    1320

tcactccaaa acagaaacga gaaatggcgg gaacaattag gtcagaagtt tgaagaaata    1380

agatggttga ttgaagaagt gagacacaaa ctgaagataa cagagaatag ttttgagcaa    1440

ataacattta tgcaagcctt acatctattg cttgaagtgg agcaagagat aagaactttc    1500

tcgtttcagc ttatttaata ataaaaaaca cccttgtttc tact                     1544


<210> 5
<211> 364
<212> PRT
<213> Artificial Sequence

<220> 
<223> NS106-2A-mGM-CSF

<400> 5
Met Asp Pro Asn Thr Val Ser Ser Phe Gln Val Asp Cys Phe Leu Trp 
1               5                   10                  15      
His Val Arg Lys Arg Val Ala Asp Gln Glu Leu Gly Asp Ala Pro Phe 
            20                  25                  30          
Leu Asp Arg Leu Arg Arg Asp Gln Lys Ser Leu Arg Gly Arg Gly Ser 
        35                  40                  45              
Thr Leu Gly Leu Asp Ile Glu Thr Ala Thr Arg Ala Gly Lys Gln Ile 
    50                  55                  60                  
Val Glu Arg Ile Leu Lys Glu Glu Ser Asp Glu Ala Leu Lys Met Thr 
65                  70                  75                  80  
Met Ala Ser Val Pro Ala Ser Arg Tyr Leu Thr Asp Met Thr Leu Glu 
                85                  90                  95      
Glu Met Ser Arg Asp Trp Ser Met Leu Ile Gly Gly Asn Phe Asp Leu 
            100                 105                 110         
Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro Met Ala Ser 
        115                 120                 125             
Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu 
    130                 135                 140                 
Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu 
145                 150                 155                 160 
Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr 
                165                 170                 175     
Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Cys Tyr 
            180                 185                 190         
Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Arg His Asp 
        195                 200                 205             
Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile 
    210                 215                 220                 
Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe 
225                 230                 235                 240 
Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe 
                245                 250                 255     
Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn 
            260                 265                 270         
Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys 
        275                 280                 285             
Val Asn Phe Lys Thr Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu 
    290                 295                 300                 
Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu 
305                 310                 315                 320 
Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp 
                325                 330                 335     
Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala 
            340                 345                 350         
Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Asn 
        355                 360                 

<210> 6
<211> 12
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 6
agcaaaagca gg                                                        12


<210> 7
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 7
agcaaaagca gggtgacaaa g                                              21


<210> 8
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 8
ctcttgctcc acttcaagc                                                 19


<210> 9
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 9
cttaaacttg caggagatgt g                                              21


<210> 10
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 10
gatgaggact ccaactgc                                                  18


