                         SEQUENCE LISTING

<110>  BioMadison, Inc.
       Tucker, Ward
 
<120>  Enzyme Assay with Duplicate Fluorophores

<130>  102320.0014

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  7
<212>  PRT
<213>  Synthetic sequence

<400>  1

Ser Gly Leu Arg Ser Arg Ala 
1               5           


<210>  2
<211>  4
<212>  PRT
<213>  Synthetic sequence

<400>  2

Ser Asn Ser Ser 
1               


<210>  3
<211>  6
<212>  PRT
<213>  Synthetic sequence

<400>  3

Ser Asn Ser Gly Gly Ser 
1               5       


<210>  4
<211>  10
<212>  PRT
<213>  Synthetic sequence

<400>  4

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


<210>  5
<211>  15
<212>  PRT
<213>  Synthetic sequence

<400>  5

Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
1               5                   10                  15  


<210>  6
<211>  6
<212>  PRT
<213>  Synthetic sequence

<400>  6

Gly Gly Gly Gly Gly Gly 
1               5       


<210>  7
<211>  18
<212>  PRT
<213>  Synthetic sequence

<400>  7

Lys Glu Ser Gly Ser Val Ser Ser Glu Gln Leu Ala Gln Phe Arg Ser 
1               5                   10                  15      


Leu Asp 
        


<210>  8
<211>  14
<212>  PRT
<213>  Synthetic sequence

<400>  8

Glu Gly Lys Ser Ser Gly Ser Gly Ser Glu Ser Lys Ser Thr 
1               5                   10                  


<210>  9
<211>  12
<212>  PRT
<213>  Synthetic sequence

<400>  9

Gly Ser Ala Gly Ser Ala Ala Gly Ser Gly Glu Phe 
1               5                   10          


<210>  10
<211>  239
<212>  PRT
<213>  Aequorea victoria

<400>  10

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Gly Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Phe Ser Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Arg His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 


<210>  11
<211>  247
<212>  PRT
<213>  Synthetic sequence

<400>  11

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Phe Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Thr Ala Cys Ala Ala Gly Thr 
    130                 135                 140                 


Ala Ala Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val 
145                 150                 155                 160 


Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys 
                165                 170                 175     


Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr 
            180                 185                 190         


Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn 
        195                 200                 205             


His Tyr Leu Ser Tyr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys 
    210                 215                 220                 


Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr 
225                 230                 235                 240 


Leu Gly Met Asp Glu Leu Tyr 
                245         


<210>  12
<211>  351
<212>  PRT
<213>  Synthetic sequence

<400>  12

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Trp Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Ile Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Ser 
225                 230                 235                 240 


Gly Leu Arg Ser Arg Ala Met Ala Glu Asp Ala Asp Met Arg Asn Glu 
                245                 250                 255     


Leu Glu Glu Met Gln Arg Arg Ala Asp Gln Leu Ala Asp Glu Ser Leu 
            260                 265                 270         


Glu Ser Thr Arg Arg Met Leu Gln Leu Val Glu Glu Ser Lys Asp Ala 
        275                 280                 285             


Gly Ile Arg Thr Leu Val Met Leu Cys Phe Pro Asp Glu Gln Gly Glu 
    290                 295                 300                 


Gln Leu Glu Arg Ile Glu Glu Gly Met Asp Gln Ile Asn Lys Asp Met 
305                 310                 315                 320 


Lys Glu Ala Glu Lys Asn Leu Thr Asp Leu Gly Lys Phe Cys Gly Leu 
                325                 330                 335     


Cys Val Cys Pro Cys Asn Lys Leu Lys Ser Ser Asp Ala Tyr Lys 
            340                 345                 350     


<210>  13
<211>  248
<212>  PRT
<213>  Synthetic sequence

<400>  13

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Leu Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Gly 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Ser 
225                 230                 235                 240 


Trp Ser His Pro Gln Phe Glu Lys 
                245             


<210>  14
<211>  719
<212>  PRT
<213>  Synthetic sequence

<400>  14

Ala Ser Ala Thr Met Ala Glu Asp Ala Asp Met Arg Asn Glu Leu Glu 
1               5                   10                  15      


Glu Met Gln Arg Arg Ala Asp Gln Leu Ala Asp Glu Ser Leu Glu Ser 
            20                  25                  30          


Thr Arg Arg Met Leu Gln Leu Val Glu Glu Ser Lys Asp Ala Gly Ile 
        35                  40                  45              


Arg Thr Leu Val Met Leu Asp Glu Gln Gly Glu Gln Leu Glu Arg Ile 
    50                  55                  60                  


Glu Glu Gly Met Asp Gln Ile Asn Lys Asp Met Lys Glu Ala Glu Lys 
65                  70                  75                  80  


Asn Leu Thr Asp Leu Gly Lys Phe Cys Gly Leu Cys Val Cys Pro Cys 
                85                  90                  95      


Asn Lys Leu Lys Ser Ser Asp Ala Tyr Lys Lys Ala Trp Gly Asn Asn 
            100                 105                 110         


Gln Asp Gly Val Val Ala Ser Gln Pro Ala Arg Val Val Asp Glu Arg 
        115                 120                 125             


Glu Gln Met Ala Ile Ser Gly Gly Phe Ile Arg Arg Val Thr Asn Asp 
    130                 135                 140                 


Ala Arg Glu Asn Glu Met Asp Glu Asn Leu Glu Gln Val Ser Gly Ile 
145                 150                 155                 160 


Ile Gly Asn Leu Arg His Met Ala Leu Asp Met Gly Asn Glu Ile Asp 
                165                 170                 175     


Thr Gln Asn Arg Gln Ile Asp Arg Ile Met Glu Lys Ala Asp Ser Asn 
            180                 185                 190         


Lys Thr Arg Ile Asp Glu Ala Asn Gln Arg Ala Thr Lys Met Leu Gly 
        195                 200                 205             


Ser Gly Ser Asn Ser Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly 
    210                 215                 220                 


Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys 
225                 230                 235                 240 


Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu 
                245                 250                 255     


Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro 
            260                 265                 270         


Thr Leu Val Thr Thr Phe Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr 
        275                 280                 285             


Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu 
    290                 295                 300                 


Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr 
305                 310                 315                 320 


Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg 
                325                 330                 335     


Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly 
            340                 345                 350         


His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala 
        355                 360                 365             


Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn 
    370                 375                 380                 


Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr 
385                 390                 395                 400 


Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser 
                405                 410                 415     


Tyr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met 
            420                 425                 430         


Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp 
        435                 440                 445             


Glu Leu Tyr Lys Leu Glu Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
465                 470                 475                 480 


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
                485                 490                 495     


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
            500                 505                 510         


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
        515                 520                 525             


Phe Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys 
    530                 535                 540                 


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
545                 550                 555                 560 


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
                565                 570                 575     


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
            580                 585                 590         


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
        595                 600                 605             


Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
    610                 615                 620                 


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
625                 630                 635                 640 


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
                645                 650                 655     


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Ala Leu 
            660                 665                 670         


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
        675                 680                 685             


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Gly 
    690                 695                 700                 


Gly Gly Gly Ser Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Gly Thr 
705                 710                 715                 


<210>  15
<211>  205
<212>  PRT
<213>  Mus musculus

<400>  15

Met Ala Glu Asp Ala Asp Met Arg Asn Glu Leu Glu Glu Met Gln Arg 
1               5                   10                  15      


Arg Ala Asp Gln Leu Ala Asp Glu Ser Leu Glu Ser Thr Arg Arg Met 
            20                  25                  30          


Leu Gln Leu Val Glu Glu Ser Lys Asp Ala Gly Ile Arg Thr Leu Val 
        35                  40                  45              


Met Leu Asp Glu Gln Gly Glu Gln Leu Glu Arg Ile Glu Glu Gly Met 
    50                  55                  60                  


Asp Gln Ile Asn Lys Met Lys Glu Ala Glu Lys Asn Leu Thr Asp Leu 
65                  70                  75                  80  


Gly Lys Phe Cys Gly Leu Cys Val Cys Pro Cys Asn Lys Leu Lys Ser 
                85                  90                  95      


Ser Asp Ala Tyr Lys Lys Ala Trp Gly Asn Asn Gln Asp Gly Val Val 
            100                 105                 110         


Ala Ser Gln Pro Ala Arg Val Val Asp Glu Arg Glu Gln Met Ala Ile 
        115                 120                 125             


Ser Gly Gly Phe Ile Arg Arg Val Thr Asn Asp Ala Arg Glu Asn Glu 
    130                 135                 140                 


Met Asp Glu Asn Leu Glu Gln Val Ser Gly Ile Ile Gly Asn Leu Arg 
145                 150                 155                 160 


His Met Ala Leu Asp Met Gly Asn Glu Ile Asp Thr Gln Asn Arg Gln 
                165                 170                 175     


Ile Asp Arg Ile Met Glu Lys Ala Asp Ser Asn Lys Thr Arg Ile Asp 
            180                 185                 190         


Glu Ala Asn Gln Arg Ala Thr Lys Met Leu Gly Ser Gly 
        195                 200                 205 


<210>  16
<211>  66
<212>  PRT
<213>  Synthetic sequence

<400>  16

Ala Arg Glu Asn Glu Met Asp Glu Asn Leu Glu Gln Val Ser Gly Ile 
1               5                   10                  15      


Ile Gly Asn Leu Arg His Met Ala Leu Asp Met Gly Asn Glu Ile Asp 
            20                  25                  30          


Thr Gln Asn Arg Gln Ile Asp Arg Ile Met Glu Lys Ala Asp Ser Asn 
        35                  40                  45              


Lys Thr Arg Ile Asp Glu Ala Asn Gln Arg Ala Thr Lys Met Leu Gly 
    50                  55                  60                  


Ser Gly 
65      


<210>  17
<211>  116
<212>  PRT
<213>  Rattus norvegicus

<400>  17

Met Ser Ala Thr Ala Ala Thr Ala Pro Pro Ala Ala Pro Ala Gly Glu 
1               5                   10                  15      


Gly Gly Pro Pro Ala Pro Pro Pro Asn Leu Thr Ser Asn Arg Arg Leu 
            20                  25                  30          


Gln Gln Thr Gln Ala Gln Val Asp Glu Val Val Asp Ile Met Arg Val 
        35                  40                  45              


Asn Val Asp Lys Val Leu Glu Arg Asp Gln Lys Leu Ser Glu Leu Asp 
    50                  55                  60                  


Asp Arg Ala Asp Ala Leu Gln Ala Gly Ala Ser Gln Phe Glu Thr Ser 
65                  70                  75                  80  


Ala Ala Lys Leu Lys Arg Lys Tyr Trp Trp Lys Asn Leu Lys Met Met 
                85                  90                  95      


Ile Ile Leu Gly Val Ile Cys Ala Ile Ile Leu Ile Ile Ile Ile Val 
            100                 105                 110         


Tyr Phe Ser Thr 
        115     


<210>  18
<211>  63
<212>  PRT
<213>  Synthetic sequence

<400>  18

Gln Gln Thr Gln Ala Gln Val Asp Glu Val Val Asp Ile Met Arg Val 
1               5                   10                  15      


Asn Val Asp Lys Val Leu Glu Arg Asp Gln Lys Leu Ser Glu Leu Asp 
            20                  25                  30          


Asp Arg Ala Asp Ala Leu Gln Ala Gly Ala Ser Gln Phe Glu Thr Ser 
        35                  40                  45              


Ala Ala Lys Leu Lys Arg Lys Tyr Trp Trp Lys Asn Leu Lys Met 
    50                  55                  60              


