                               SEQUENCE LISTING

<110> UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR 
      COMMERCIALIZATION
 
<120> METHODS OF MICROBIAL PRODUCTION OF EXCRETED PRODUCTS FROM METHANE
      AND RELATED BACTERIAL STRAINS

<130> 034186-082620-PCT

<140>
<141>

<150> 61/892,909
<151> 2013-10-18

<160> 8     

<170> PatentIn version 3.5

<210> 1
<211> 4076
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide


<220>
<221> primer_bind
<222> (1)..(20)

<220>
<221> primer_bind
<222> (721)..(740)

<220>
<221> misc_feature
<222> (741)..(3461)
<223> Gene deletion region

<220>
<221> misc_feature
<222> (752)..(1351)
<223> Coding region

<220>
<221> misc_feature
<222> (1348)..(2736)
<223> Coding region

<220>
<221> primer_bind
<222> (1423)..(1443)

<220>
<221> primer_bind
<222> (2439)..(2458)

<220>
<221> misc_feature
<222> (2739)..(3461)
<223> Coding region

<220>
<221> primer_bind
<222> (3492)..(3512)

<220>
<221> primer_bind
<222> (4056)..(4076)

<400> 1
tagaacgccg cccgataaag ccgcgtcgct cttcgaaatg cttaagacaa aaggcatctt       60

aatcaaaaac ctgtcgcctc aaggcggcct cttactggat tgcctacgcg ttacgatcgg      120

cacgcccgat gaaaatgacg cctttttgca agcgttatcg tcaagtttcg aacaactaaa      180

tgggtagcga gaaaaattcc tatcataacg tgtgctttca tatacttttc gtatttcttg      240

gcaaaacaga aacattgggg acgggttaaa taacccgtcc cgcagggact ttatgccagc      300

tccatgctgg caaagccttt atgaacgcca tggatggcgt gtattagggc aatgcaggag      360

caattaccga ggagcgaaaa tcgaccggac acccaggcat ctcctctggc actgccgaaa      420

tttgaagtgc gaaaggtata gcttcctgaa actttgggct ttgtagcggg ttcggtaact      480

cgcttgacag gatgccgccg gcaccgaatt ttgatctaag atgggtataa aaacgtattg      540

ctgcttgatt tcaaggattt aaataccaat ccttataaaa acaagacgca taaacactgc      600

tcatacaaag aaagcggttg aaaaacccga aaaacgaatt aatcaaccgt tataactgct      660

tcaaatttcg ctaactataa tggtatattg cgatgtcgaa aactataaaa atcaagtata      720

cgaggctcag cctttaccag gagtgcaata aatgaatatt gaaggcgtcg ataaatccaa      780

gcgccagttt ctaacctcgg cgctgaccgt ggtaggagct gtcggtaccg gctatattgc      840

ggttcccttt ctcgcgcaaa tgcaacccag cacaaaagct atggccgcag gcgcgccggt      900

caccgtcgat atcagtaaaa tggaaccggg acagctaatc cgtgttgctt ggcgcggtaa      960

accggtttgg cttcttaacc gcacaccgga agtgctggaa acactgaaaa cattggacga     1020

ccaactcaga gaccctcagt cttcagaatc catacaaccg gtgtacacca aaaacccaac     1080

tcgcgcatta aagccggaaa tttttgttgc cgtcggctta tgcacgcatc tgggctgttc     1140

gcccaccttc cggcctgaaa tcgcaccggc cgacctgggc cccgactgga aaggcggttt     1200

tttctgccct tgccacggct cttggttcga tctggccggc cgggtttttc gtggcgttcc     1260

ggctccgacc aaccttgaaa ttccacccta tcgctatatt agcgaaactc agatcatcat     1320

tggcgaagac accgtggaga caagcgcatg aaaacgaacc gctggactga ttgtggcaac     1380

tggatactgg atcgttttcc gctgacaaag ttctggaacg aacacatggc gcattattat     1440

gcgcccaaga attttaactt ttggtatttt ttcggctcgc tcgccttgct ggtgttagtc     1500

aatcaattcg tcaccggcat attgctgacg atgaactata aaccggacgc taaattggcc     1560

ttcgactcag tcgaatacat catgcgcgaa gtcaattggg gctggttaat ccgctacatg     1620

cactcgaccg gtgcttcggc atttttcatc gtgatctatc tacatatgtt tagaggctta     1680

ctctacggtt cttttaaaca gccgcgtgaa ctaatctgga ttttcggcat gctgctgttt     1740

gttgctttga tggccgaagc gttcatgggc tacttgcttc cttggggaca aatgtcttac     1800

tggggagccc aggtcatcat ttcgctgttc agcgccatcc cggtcgtcgg agaagaactc     1860

tcattgtgga tcaggggcga ttatgtcgta tccgatgcaa ctttgaaccg attctttgcc     1920

tttcacgtca tcgcagtccc tttggtgctg gtattgctgg tattgctgca tatcatcgcg     1980

ttacacgaag tcggctcgaa taacccggac ggcattgaca tcaaaaaaca caaagacgcc     2040

aacggcatac cattggacgg cattcccttc cacccttact acactgtcaa agacttggtc     2100

ggcttcgcgg tcttcatgtt cttttttgca gtcgttattt tttatatgcc ggaactggga     2160

ggattcttcc tcgaacacgc caatttcata cctgccgatc cattgaaaac gccggaacat     2220

atcgctcctg tctggtactt cacccctttc tacgcgattc tgcgtgcggt cccggataag     2280

tttgccggtg tcattgcgat gggtggctcc atagctgtgc tgttcctgct accgtggctt     2340

gatcgcagca aagtacgctc aatccgttat cgcggcggca tctataaaaa agcgctattg     2400

cttttcgtaa tcagctttat cggcctaggc tatctgggca ctcagcccgc aacgcccggc     2460

gctaccgctg tcgcacgcgt tttaacggcc atttatttcg gttttttctt actgatgccg     2520

atctataccc gctgggaaaa aaccaagcct gtaccggacc gtctaaccga acaacctagc     2580

ctcgaggatc gaataccgga ttttgttgaa actttcaatg aagttacggt gaaaacagag     2640

ggaaaatccg gcctgttcag cagaaaactc aattaccaag ggttatccgc cgttagcatc     2700

cgttttttaa aaaaaattaa aggcagcttg gattaattat gaaaagatta ctcaatacct     2760

tgatactgtt gtcgatgtct ttcggcgtgt tcgccggagg cggcgtcgaa ctccagagcg     2820

ccgatttcga cctcagcgac aaaggttcgt tacaacgcgg cgcaaaacat tttgtgactt     2880

attgtttagg ctgccattcg gtgaaacata ttcgttatct caggatagcg ctcgactttg     2940

atattgaaaa agacaaagta ctcaaagata tcgcgccgga aggcgccaac atctacgata     3000

agatgaattc agcgatgaac gcccatgacg ccgaaaaatg gttcggcatc atgccgcccg     3060

acttatcgct gatttcacgg tcacgcggac cggattggct ttacagttat ttgaaaggct     3120

tttattccga ctcgtccaag cctctcggcg tcaataatgt cgtttttccg gatgttggta     3180

tgccgaacgt tttatggcaa ctgcaaggcg agcaaaaacc ggtattcaaa aaagtcggcg     3240

gccaacaagt cattgacaaa ctagctaccg aaattcccgg acaacttccg gaaaaagatt     3300

ttgatcgcat gattaacgat ctagtcaatt tccttgttta tgtcggagag cctggacaac     3360

tcgagcggcg aagcatgggc aaatatgttt tgtttttcat tttcatgatt ttcatacttg     3420

cctatctatt gaaacgcgag tattggaagg acattcatta atcccacatt cgtaaaacag     3480

ggacgtttta cccgtatcat ctaccttcat acggatacag caattcccag tttgagcatt     3540

ttcgccgatc ttagggtatg gggtatgcct gtcgaggaac gccgtaaacc catccatggg     3600

ggcttggcgg cagctaagca ccaaggatgg catgtagtag ggcagtgcag gagcaattgc     3660

cgaggagcaa aattctgccc ctgctgccga tatcctcgct aagcacaccc caaacccttt     3720

ttgatcccca aattgggaat tgctgatacg gatacaagca aataaaatgt cgaatctttc     3780

atgatataat cgctatttta gactccgtgt atcctaatga ggttatgatt cgtggcacaa     3840

ccaactaccc gtaaatccgc tatgactctt ttttcatctc cgacatgcgc gatgagccac     3900

ggcgcccgat tggttcttca tgagaaagcc attgcggcgg acattgaata ttatgacatc     3960

aaccaaccgc ctgaggactt attagagttc aatccaacag gcacttcgcc aaccttgctg     4020

gaacgcggac tagtcctgta tgatgcgcgc atcatcatgg aatatctgga tgaacg         4076


<210> 2
<211> 2662
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide


<220>
<221> primer_bind
<222> (1)..(22)

<220>
<221> primer_bind
<222> (572)..(596)

<220>
<221> misc_feature
<222> (597)..(2021)
<223> Gene deletion region

<220>
<221> misc_feature
<222> (844)..(2049)
<223> Coding region

<220>
<221> primer_bind
<222> (2022)..(2045)

<220>
<221> primer_bind
<222> (2641)..(2662)

<400> 2
caaccgaaca ccgaacatcc tcacggttta agtcatcgcg aattcgatag cctattcacg       60

gtcgacaaac cggtcatttt caatttccac ggctatccct ggttgattca caaattggcc      120

tatcgcttca agaatcatga aaatctgcat gtgcggggct ataaagaacg cggcaatatc      180

aatacaccca tggagcttgc gattctgaac gaagtcgatc gcttcaatct agtcatcgat      240

gtcatcgacc gggttccgaa attgcaaatc agggcggccc atctcaagga gctcatgaaa      300

aacgaaatca tcgagaatct tcgttatgcg catcaacacg gcaccgataa acccgaaatc      360

accggttggc aatggccgtt ttaacagtaa tcctgaaaag agcagttggc ctatgttgta      420

gaccgttcgt gctgagccaa gtcgaagcat gaagggtcca caatactttc accggactgg      480

tgaagcctca aactaccgtt cacccttcga caaggctctc ctgagcgtag ccgaaggggc      540

tctcaacagc tctttttagg gtaatgacaa acctgattag caagatgctt cagcttgctg      600

aagccaattg tccgagcagt ggccagtcgt agccacttct gcgggacggg ttatttaacc      660

cgtccccaac gtttcggttt gccctaaaca tttcggctaa cttcggccaa agtcaaaacg      720

tttaggacgg ggttgcaaac cccgtcctgc tagggatatg ctggtttttg ggctttagct      780

gaagaaactt gctaatcagg tttttatttg taaaacagcg tttaccaaaa tcattttaag      840

gatatgacaa taccaaacgg caacattctc gtgatcaaca gcggcagttc ctcaatcaag      900

tatcgattga ttgctctgcc gcaagagcag gtactggcag acggcttgct ggaacgcatc      960

ggagaacagg aaagcaggat cattcataga gccgacgatt cgggtcgttt aaatgaaatc     1020

aagcagtcgg tcatcgctgc cgatcatcac caagccttca aggcagtctt cgagattttg     1080

ggcgaaaatt gctcggtcga tgcaataggc caccgcgtcg tgcatggcgg cgatcggttc     1140

tccggccctg ccttggtcga tgacgatacg atagcgtcga tgcgcgcact ctgccgaata     1200

gcgccgctgc ataatccggt taacttgctt ggcatcgaga gttgcttggc tcatttcccg     1260

ggcgtaccac aggtggcggt attcgatacg gcatttcacc aaacgatgcc gccccacgcc     1320

tatcgttatg cgattccgga aacttggtat agcgattacg gcatacgccg attcggtttt     1380

cacggcacct ctcatcatta tgtggcgaga cgggccgccg aatttatcgg taaacctttc     1440

gatcgcagcc atctgattac tttgcatttg ggcaatggcg cgagcgcaac agcgattgca     1500

aacggccgct ccgtcgatac gtcgatgggg tttacgccgc tggaaggttt ggtaatgggc     1560

acgcgtagcg gcgatttgga cccggcaata ccgctatttg tcgaacaaac cgaaaatacc     1620

gacacggacg caatcgaccg ggcattgaac cgcgaatccg gattaaaagg cttatgtggt     1680

accaacgact taagaaccgt gctcgaacaa acaaatgcag gcgatgaacg agcccgcttg     1740

gctctcgatc tgtattgcta tcgaatcaag aaatatatcg gcgcttacta cgcggtactc     1800

ggcgaagtcg acgctctggt ttttaccggc ggcgtcggcg aaaacgcggc cgaagtgcgc     1860

cgtttagcct gcgaaggcct gtcgcgtctc ggcatcgcca ttgatgaagc ggccaatagc     1920

gacgtgaccg gagctatcgc cgaaatcggg cttgccgaaa gtcgaacccg tattctagtc     1980

attaaaactg acgaagaatt gcaaattgcc cgggaagcca tggctgtgct tgataaagat     2040

cacgcatgaa aatatttaga ctgaacggtt taccgggcac ccaacaccat ggaaaaattc     2100

ttcaacacgt taggtcccat aaaagcggcc atgcattatt atattccgcc gctggatcgc     2160

atcgattgac ctaaaattca acacatagct tacgtaggat gggtagaggc ggaagccgaa     2220

acccatcgtt tcaaccccac caccgaatct ctcgccgaac ggattttata aatccagccg     2280

caatagtttc ctgccgtttg cggggtctcc atgatgggtt tccgcttcgc tcctacccat     2340

cctactgcct ctttcatcct tcccctttct tctttcctct ttaacctaaa ttaaacatct     2400

ccccataacc ctcccgataa gtcgggtatt taaaccgaaa ccccaaggtc ttcaagcgac     2460

tattccgaca tcgtttgttt tgatccggaa cattatcggc aggttttgcg atcggcggtt     2520

ggctgttcat ttgttgagcg agccataaaa tcacgtccca catcggcgcg ggattgtcgt     2580

cgctggccaa ataaaaattg tccagatgcc ctccttcgat atgctttttc attaaaaaag     2640

ccaacacagc cgcgcaatcg tc                                              2662


<210> 3
<211> 2887
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide


<220>
<221> primer_bind
<222> (1)..(20)

<220>
<221> primer_bind
<222> (703)..(723)

<220>
<221> misc_feature
<222> (724)..(2208)
<223> Gene deletion region

<220>
<221> misc_feature
<222> (745)..(2238)
<223> Coding region

<220>
<221> primer_bind
<222> (2209)..(2228)

<220>
<221> primer_bind
<222> (2866)..(2887)

<400> 3
gcttggaaac ggagcatgag agccatgact aataaaatca gagtcgcaac cacatcgcta       60

gccggctgtt ttggttgcca catgtcgttt ctcgacatcg acgaacgcat gctgcaactc      120

gccgaggcgg tcgaattcga ccgttcgccg attaccgaca tcgaacattg cacgaattgc      180

gacatcggct taatcgaagg aggcgtctgc aattcggaaa acgttcatgt gctgcgcgaa      240

tttcgtaaaa actgcaaaac actggtcgcg gtcggcgcct gcgccatcaa cggtggcttg      300

ccggcgatgc gcaactttat accactcgaa gaatgcctta gcgaagcgta cttagacggt      360

attggcgtcg aaaacccgca gattccaagc gacccggagc tacctttatt gctggacaag      420

gttcgcccga ttcatgaaat cgtcaagatc gactatttcc taccgggctg cccgccgtcg      480

gccgatgttt tttggaaatt tttgaccgac ctgctcgaag gccgcgaacc gtcactgcct      540

tacgagttgg tgcattatga ttgatgtgta ttctcgcgct gagttaattc gaaaggatta      600

attatttccc gcaaaggcgc tgagaacagc attattaaga tctcggcagt ccacagggtt      660

tgcgcgatga accctatcat tgagcgaacc atgaaataaa tactgcgcct ctgcgcgagt      720

caataaaatt attcgagaac acttatgtac gaacatttag aaacagccgc cagcccggaa      780

aaccttaaac gtgtcgtggt cgatccggta tcgcgggtcg aaggccatgg caaagtcaca      840

ttattgctcg atgaaaacaa taaggtccaa caggcccgct tgcatatcgt cgagtttcga      900

ggtttcgaac gcttcattca aggtcgtcct tattgggagc tacctgtttt agtgcagcgc      960

ctatgcggca tctgcccggt cagtcatcat ttagccgccg ccaaggcaat cgatcagctc     1020

gtcggcatcg acccggaaaa tctgcctcca gccgcaacca aattaagacg cttgctgcat     1080

ttcggccaag ttatgcagtc ccacgcctta cattttttcc atttgtcgag cccggattta     1140

ttgttcggct tcgaaagcga gatcggcaaa cgcagcatca tcgcggtatt ggccgagcac     1200

cctgaaatcg gcgtacaggg tgtcaagctt cgcaaatacg gacaagaagt aatccgcatg     1260

atttccggca agcgcattca cggcaccgga gcaatcgccg gcggcatgaa caaaccgtta     1320

tcccgtgaag agcgcgatta tttgttgacc gatatcgatc aaatgatcga atgggcggca     1380

gcaacggtac aactggtcaa aagagtccat tgttcgaatc tgccttatta cgacgatttc     1440

gcgacgatac gcagtaatta tctgggcctc acccgtccgg acggtgcgct ggagctttat     1500

cacggaggtc tgcgcgcgaa aagagacaac ggcaaaacca tcctcgacca tgtcgattat     1560

tgtcgctaca acgattatat tcatgaagaa gtccgttcct ggacctacat gaagtttcct     1620

tatttgttgt cggaaggtaa ggaaaacggc tggtaccgcg tcggcccact ggcccgcgtc     1680

aataactgcg actatatcga caccccgttg gccgaggcgg ctcgcatcga atttaaacaa     1740

cacggcggtg aagcaatggt tcatagcacg ctggcaaccc attgggcgcg catgatagaa     1800

acgctacact gcgccgaaag catcaaagtc ttactgaacg acccggaaat catgagtaac     1860

gacctcgtcg cgcaaggtga aaagcgattc gaaggcatcg gcgtgatcga agcccctcgc     1920

ggcacactgt ttcatcatta tcaagtcgat gaaaacgaca ttgtcacccg cgccaatttg     1980

atcgtttcaa caaccagcaa taacatgggg atgaacgaat cggtccgtca ggtcgctgct     2040

gaatatttaa acggtcgcga attgaccgaa ccgctattaa ataatctaga agtcgcgatc     2100

cgcgcctacg acccttgttt atcttgcgca acccacgcgg tcggaaaaat gcctttgcaa     2160

ttggagttaa tcgacgcgga cgggcaactc atcgataaat tagttaaaca tagcgatggt     2220

gttattactc ccttttaatc gaaaaaaccg tctaaggaat atacacaaaa taacttatac     2280

aagtagtagg ctttgtagcg gtttggtgac tcgcttgaca ggacgccgtg aatacgtcca     2340

ttttgtcaat ggccaacaga attgagccag ttttggccat ttaaattgag ccacttttcc     2400

agggttacga tgcttgattt agctttgatt tgagtcgata actttcccct cccagcataa     2460

aaatgtgtga atgatggatg atgcggtcga cgatgggtac ggcgacgttg tcatcgataa     2520

aaaggctcta tgtgaaatac agtgggtaat caaatttcaa ctttccacat aggaagtaaa     2580

tcatattaat gaaattgttg atgttgcgat agcctctggc tcgccgttta gccagctgaa     2640

tcttactatt gatgccttca agaatgccgt tactaatgag tgattccacg aaatgcacaa     2700

ttcccgacca atgtcctcga actgtgttgg cgaacttgat aaacgctgga attttggctg     2760

cgtccaccgc atcgcaccat tgcctcaaaa aagcctccgc tgtcggtttg tcgggcattt     2820

cccagagatc attgaatagc acttttaagc gataagcttc gcccagcgtg gggtacagtt     2880

gtatcag                                                               2887


<210> 4
<211> 3070
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide


<220>
<221> primer_bind
<222> (1)..(19)

<220>
<221> primer_bind
<222> (670)..(687)

<220>
<221> misc_feature
<222> (688)..(2466)
<223> Coding region

<220>
<221> misc_feature
<222> (691)..(2451)
<223> Gene deletion region

<220>
<221> primer_bind
<222> (2452)..(2472)

<220>
<221> primer_bind
<222> (3051)..(3070)

<400> 4
ccataagtat ctacacatct ttatcattta aaatcatgct gttataaagt ttcttctggt       60

ttattatgaa gatagggact aaaaatttag gtttttaatc tactttttta tcctagatca      120

cagaaaaatt aacttaacta attgttttat aaagctgaaa acttgtgtag atacctatgc      180

gctccggcgt gggaatgaag accgagacga tctagcgtct cgtaccgctg gagcggtact      240

caggcgttcc cacgcagagc ctcactgcca ttaagttaag ccgttcgtgg tgagcctgtc      300

gaaccatgga cgccttaact tatcgaccca ggcttttcca ttcacccttc gacaggctca      360

gggcgaacgg aaaaatcctt aacttaatgg tagtgacgca gagcgtggga acgataattg      420

ccggggggct gaatagttac gccgtgaatg ttttagttta ttcacgaaaa tcgtgagcat      480

ccctgagtcc ggaatcgcaa ctttcaacgg agatagcgag gtcataccag ccgaataacc      540

cttatacaga gcccaactga caaactatgc tgaaattgcc aacattcgaa aacactgtgt      600

tcactctatg taatggtatt ttttttaata ttggcatata gttggactta agccctccgt      660

cataacccag cgcagcagag agacaccatg gaatcgttca ttcaaaatct cgttgaaacc      720

cacgcaaggc gcccaactcg gcttttagca atattacgtg ccgttcaggc gcgatacttc      780

cacattccga accaagccat tcgattgtta tccaaggaat taaacattcc aacaacacgc      840

attatcggcg ttatcgaatt ttatagcttt cttcatttaa cgcctcgcgg acgttacgat      900

atattgatca gcgattcgat taccgatcac atgctcggca aacacaactt gatggactat      960

ctatctaaca aactttccgt gtcacccggc agcgttcgtg aagacggctt ggttagccta     1020

gataatactt catgcaccgg catgtgcgat caaggccctg ccgggctcat caatggccac     1080

gcgatcactc gacttgaccg gcctagaatc gacgcgatcg cacaattaat cgacgaacaa     1140

atggcagttg aggattggcc caaggaattc tttgaggtcg gcgataacat ctatcaatcc     1200

gatttattat tgagcagtcg ccttaccgcc ggagaaggtc tacaatcgac tttcgcacgc     1260

ggcctaaaag aaacactatc ggaaatcgat caatccggac tcagaggccg aggcggcgca     1320

ggctttaaaa cggcgatgaa atggcgattt tgcgccgaag aatcggaaca agaacgctat     1380

gtggtctgca atgccgatga aggcgaaccc ggaacattca aagaccgagt attactaaat     1440

agctatgccg accatgtttt cgaaggcatg actatttgcg cggcacttat cggcgccaag     1500

cgaggatttc tgtatttacg cggcgaatat cttcaccttt atcacaaatt agaaaacgtt     1560

ctcgaacaac gccgtcagaa cggtttgctc ggtaacaata ttctcggcaa aggcctagat     1620

ttcgatatcg aaatttgcct cggtgccggt gcttatattt gcggcgagga atcggcactc     1680

atcgaatcgc tcgaaggcaa atgcggtatt ccgcgaaacc gcccgcccta ccctgtttcc     1740

caaggctatt taaacaagcc gaccgtcgtc aacaatgttg aaactttcgc agccaccgct     1800

aacattgcct tacacggggg cgcttggttc gcagctaaag gcacggaaaa atcccaaggg     1860

accaaaattc ttagcattag cggtgattgc gcacgtcccg gcatttacga atatccattt     1920

ggcgttacga tacggaaaat tctggaggat tgcgatgccc atgatgtact cggcgttcaa     1980

atcggcgggc cttccggcac ctttatttcc aaccaggaac tcgatcgcat actaggattc     2040

gaggatttag ccacgggcgg ctcattcatc gtttttaatt cgacacgcaa cattctcgat     2100

atcgtgcgta atttcactga cttcttcgcg catgaaagct gcggtttctg cacgccttgc     2160

cgagtcggta cttcgttatt gaaaaaacag ctcgacaaaa tcgtcgacgg ccacggttcg     2220

gccggcgatg tggttgcact tgaagaacta actcgcctag tcaaaaacca tagccattgc     2280

ggcttaggcc aaacggcggc caacccgatc attcatacct tagagaggta tccggactta     2340

taccaaagcc aattgaaaaa aatcagttac gagcccggct tcgatttgaa cgcttccctt     2400

gaaactgcca gacgcatggc aaaccgtaat gatgcagggg cacatctagc ccaggtggag     2460

gaatagcatg agcaaaacat ttaccttgga cggcaagccc attccattcg aagatggaca     2520

aaccatcatg gatgccgcta ctgcggccgg cgtttacatt ccgcatcttt gtcatcatcc     2580

ggaattcacg ccgcacggca gctgtaagtt atgcaacgtt aatgtcaatg gccgcacttg     2640

ttcggcttgc acattcccgg caactgaggg ccaagatgta ttgagcgaaa cgaccgaaat     2700

caatcaggac cgccaaaaaa tcacgcaaat gcttttcgtc gaaggcaatc atttttgccc     2760

gtcctgcgaa aaatccggga actgccaatt acaaggggtc gcataccatt tgaatatgct     2820

cgacaatcat tttccgcatt tttacgcgga acgtaaaatg gatgcgagcc actcggacgt     2880

catgatcgac cataatcgct gcattttttg caccttatgc gttagggcca gccaacaagc     2940

cgacggtaaa gacgtttttg cgatttcagg ccgcggtatt aacaagcact tgatcgttaa     3000

ttccgagtcc gggcagttaa aagacagcga tatcgatccc agcgaccgag ccgcgcacat     3060

ctgtccgacc                                                            3070


<210> 5
<211> 2273
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide


<220>
<221> primer_bind
<222> (1)..(22)

<220>
<221> misc_feature
<222> (690)..(1613)
<223> Coding region

<220>
<221> primer_bind
<222> (712)..(730)

<220>
<221> misc_feature
<222> (731)..(1595)
<223> Gene deletion region

<220>
<221> primer_bind
<222> (1596)..(1617)

<220>
<221> primer_bind
<222> (2255)..(2273)

<400> 5
caggatgcgt gccgaaataa gcttgagtga cttgttggtt cagttcctct atttctagcc       60

gctctttcgc caacaactct cctttaatgg cgatgatttg gcttaaataa tcctcgactt      120

tattgcggac tttgttgatg cgttggatat gaatcgtgtc ttgttcggag atttcataaa      180

atttgaaata agaaaacgac accgatgcac ccaatgcaat caatagcgag gcaagcacgc      240

tgaaataacg caatcgattg gcgcgccagt gaatcccggc gatctcgaat gagcaaatca      300

cgatgatcga ttgaatcgcg accgtgatga tcagcgcgat ccagggtgtg atgaaatgcg      360

acatgccgta atatgtcgta aacccggaag ccaaactgag cgcgagaacg atgggaatgg      420

tccatattcg catcagactc acgaataggg tttgtatgat gttgatgaga cgtcctaggc      480

cgccgctttt catttttatt attattcggt tgtccatcgt ctgactttat gttgttgttc      540

gagtatgcgt tattacggaa attagcagga tcaaaaaaat tctgctaata gtatcacatt      600

gaatttaaat agtttctatt gaaaataaat caatcggttg tctttatccc gtcagttgcg      660

ggagcgtttt atcatagtga gtatcaatca tgaaaatagc cataatcgga gccggccatg      720

tcggttcttc gttagcctac gcattggtgt tgaagggctt gggtaatcat ttgttgttag      780

tcaatcgcga tgcgaccaaa gctttgggca atgccttgga tttacagcat accctggctt      840

tttgcgaacg gccgatgcaa atcgagggcg gctcgcttga agaggcggtg ggctgcgata      900

tcgtcgcgat caccgcttcg gagccgatga caaaaggtat gacttctcgt atggaattag      960

gacaagccaa tgtcgagttg ttcaagtcgt tgattccaat gctggccgaa aataatcctg     1020

aagctgtttt tattatcatc acgaacccgg tcgatgtgat gacttgttgg gctacgcggg     1080

tttcggagtt gcccccttca agaatcgtcg gcatcggcac gctcgtcgat tcggcacgtt     1140

tcagaaccct attatccaag gccgaacaga ttcatcccga tgatttaaga gcctatattc     1200

ttggcgaaca cggaccgaat cagtttccgg tatttagcca tgcttcggcc ggcggcgaac     1260

cgataaccga tagtccaagg catagagaga tttttgaaga ggtcaacaag gccggtttcg     1320

aggtttatcg attgaaaggc tataccaatt tcgcgattgc ttcagccgct tgcgaagtca     1380

ttagaaccat cgttcacgac gaacaccgaa cgatgccgct cagtacttgc ttcgatgatt     1440

ggcagggaat caaagacaat tgcttcagca ttccggtcgt actgggtcgt tccggtatta     1500

tccgatattt acagcccgac ctgaacagtc tggagcgcga agctctggtg catacggcca     1560

aaattgttcg agctaatatc gatagtttat tgtttggagt aggtgaaagg tgaaaggtga     1620

aaggtgaaag gggaaagggg aaaggggggg ggcttgatag gggtagggaa gatttctctc     1680

ccctccctcc gaaccgtgca tgcggttctc ccgcacacgg ctctccagtc ggtggtttcc     1740

tcatcgggac aagctggtga gcaatggtgt tattaattgg tgaaccccat tactccgcgt     1800

taccacgtca gcccgcaaac tcggtttgat tcgttacctt cggctccctt caacggctga     1860

gcctctttaa gccttttacc tgaggttggc ttaatggccg tgacaccgaa cgctacctcg     1920

tttccacctt cctacccttc tttcctatgg ccggttttgc tagccggctt ttaccatctt     1980

ttcccctctc ggtttaggct cgtgtttgtt ttctctgttt ccgcctggtt tcccagcggt     2040

gccacactat cctcgccttg ccttgagctt cctcgactta cggtaatata agggttctga     2100

ctcctgccac ggtcacctcc gtaacaggtc tccccagttt cttcacaata ccttctgaac     2160

attccactcc caaccacaag gtaggcccat ttatcgtttt atctgccaca acaacgtaaa     2220

tgatggtttt caggcttcgt cgactcccag gggactcgcc gccttaccct gcc            2273


<210> 6
<211> 3353
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide


<220>
<221> misc_feature
<222> (1001)..(2353)
<223> Gene deletion region

<220>
<221> misc_feature
<222> (1001)..(2353)
<223> Coding region

<400> 6
aatcgattgc acgacgaatg taaggatcaa ggcaaaaatg tgcatttcat gtcgccgacg       60

gtatgcatgt gttctacgat gttccgaacc gatccgcagc atttggcttg ggtgctggaa      120

aatctggtcc aaggacatgt cgttaatcgg atatcggtac cggaacgaga gtctgtacta      180

gctaaaaaag cattggacaa tatgctttcg atacattgat atcctcttaa gaagtaggca      240

tccaatgact ttggtatttt attatactca tcgtagatga aaattcggcc tcggggtgcc      300

cgttaaagga cgccgtaaat ccagcaccta aatattccat aggtctttgg caatgattca      360

aaatcatggc ttatttaggt gctggatgaa ttatccaaga caattaacca tggccaatgg      420

gctatggaat ttaggtgctg ggtaaatacg tccatgtagg ctctatgcca gctccatgct      480

ggcaaagcct ttatgaacgc catgggtggc gtgtattagg gcaatgcagg gcagaaaaat      540

gctcctgcat tttctgcatt cattacatcc atgtaactca gcaattgcca aggagcgaaa      600

atcgaccgga caccccgagg cctcctccgg cactgccgaa atttgaagtg cgaaaggtat      660

actctgatta gcattaaaga tctttattaa tgataagaaa ctttaatgct gtgcttttta      720

tatagcatcg aatcgataca aaaaagccgt tacttacttg gtatgtaacg gcttttttgt      780

ttttgaatat tgtgtcgaaa aatttcaatt cataacaata agccgaaata tgcgtgatag      840

gtgacagtgt gttgactcaa aggacatttt atcgatcttg agaacgaaac gaaattatgg      900

tctaatgccc cggtttacgt cttcaagtgc ctcttatttc tgctgtttca aataaggggg      960

ttgttcgccg cgatatgtct ggagagcagg cttatttact atgcaattta atttaaaaaa     1020

aggtttggat cttccgatca cgggagagcc cgaacaaata atagaaaacg gtaataaggt     1080

taagtcggtt gccgttcttg ggatggatta tgtaggaatg aagcctacca tgatggtagc     1140

cgagggtgac aaagtcaaac tcggtcaagt gcttttttcc gataaaaaaa atcctggggt     1200

caatttcaca tcgcccggtg ccggtatcgt taaggcgata aaccgcggag ccaagcgcgt     1260

tttgcaatcg gttgtcatcg agctgaaagg taatgaggcg gaatcgtttg caaaatataa     1320

agattccgaa ttggacggtt tatcaaccga gcaagtcaag gaaagcctca ttgcttccgg     1380

tttgtgggtt gcgttgagaa cgcgtcctta cggaaaaaca ccggcaatcg atagcactcc     1440

tagttcgatt tttgtgaccg caatcgatac caatcctttg gccgccgatc catcggtaat     1500

tatcaaggaa cggtctaccg actttagcaa cggtctggca gtgattgcta agttgaccga     1560

tggtaaagtt tacgtctgta aagcctccgg tgccgacata ccgaccggaa acgcgtcttc     1620

cgttacagtt gccgagtttt caggtccaca tccggccggc ttgcctagta cgcatattca     1680

ttatatcgac cctgtcaacg ccaataaatc ggtttggcat ttggattatc aagccgtgat     1740

ggcaattggt tcgttgttca cgaccggtaa attgaatgtt gaaagagtgg tttcgttggc     1800

ggggcctacc gctaaaaaac caagattgct tagaacccgt gtcggtgcat gtctcagcga     1860

actcgtcgct ggagagctga tggacgggga gaatcgcgtg atttccggtt cggttatcta     1920

tggccataaa gcggcagatt ggtcggatta tttgggttat tacagcaatc aagtttcggt     1980

attgaaagaa ggccgtgacc gagagttctt cggttggatc gttccgggca aggataagta     2040

ttcggcctta aatgtataca cctccagtaa agaccgaaaa atcggacgca agtttccgct     2100

gacgacgaac aaaaacggca gtaatcgggc cattgttccg gttggcgtct acgaatccgt     2160

gatgccgatg gatattttgc ctacgccatt gctaaagtct ttggttgtcg gcgatacgga     2220

tcaagcgcaa atgctaggtt gtttggagtt ggacgaggaa gatatggctc tgtttacctt     2280

tgtcgatccg ggtaagcatg agtttggtcc ggttctgaga gcgaatttaa ctaaaatcga     2340

gaaggaaggc taatgtcggc tagaaatttt ttagacaaca tagagccgca ttttaccaaa     2400

ggcggtaagc tagagaagta ctacggtctc tacgagatgg tcgatacttt tctatatacg     2460

cccgctgagg tgactcgcgg taaaactcat gttcgtgacg gtaacgattt aaaacgaaca     2520

atgacgtttg tcgttattgc aacatttttt tgtatcctga tggcttggta taacaccggc     2580

tatcaagcga atgtcgcgat ggaagcgatg ggcctggaaa aagcggacgg ctggcgtagt     2640

ctgcctatga tgatattcgg atatagccca tacaatccgt tatccaactt ggttcatggc     2700

gcgctttact ttttgccaat ttatatcgtg accctggctg tcggcggtgt ttgggaagtc     2760

ttgtttgcga ccgtgcgcgg tcatgaagtc aacgaaggct tcttggtatc gtcgatgctt     2820

tatacattga tcatgccgcc cgatatgccg ttgtggcaag ttgcgctggg tatttcgttc     2880

ggtatcgtaa tcggtaagga agttttcggc ggtaccggta aaaatttctt aaaccctgct     2940

ttgaccggtc gtgcatttct gtacttcgcg tatccggcat ctatttccgg tgactccgtc     3000

tgggttgcgg ttgacggtta cacggctgct acgcctttag gtttagccgc ttcaggtggc     3060

ctggatgccg tccaagaggc cggttatggt tggatgcaaa cattcttcgg tttcatgccc     3120

gggtgtttgg gcgaaacctc gacggtggct attttgattg gctgtgcgtt tttgctctac     3180

acccgtatcg cctcttaccg tatagtgggc ggtgtgttcc tcggcatgtt gatgatgtcg     3240

tcggtattca attttatcgg cagcgacagc aacccaatgt ttgctttccc atggtattgg     3300

catttgaccg tcggcggttt cgcattcggc atggtctaca tggctaccga tcc            3353


<210> 7
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xHis tag

<400> 7
His His His His His His 
1               5       


<210> 8
<211> 1992
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide


<220>
<221> primer_bind
<222> (1)..(19)

<220>
<221> primer_bind
<222> (516)..(536)

<220>
<221> misc_feature
<222> (597)..(992)
<223> Gene deletion region

<220>
<221> primer_bind
<222> (1063)..(1085)

<220>
<221> primer_bind
<222> (1565)..(1587)

<400> 8
tttcgccgca tttggctgga gcagagcatc cgccggtttt ggatgatata tgttcgattt       60

ttcaaacgct gcaacaaaat gccgatatcg ttttggtcga aggcgtcggc ggttggcggg      120

ttccgttgaa tgccgactcc gatgtctccg atttggcgcg cgtactgggc ttgccggtcg      180

tcatcgtggt gaagataagg ctgggttgta tcaatcatgc aagattaact tatgagtcga      240

ttgtgcagtc cgggcaagct tgcgccggtt ggatagccgt ttgctccgat aacaacatgc      300

tgttgcgcga ggaaaatatc gccacgatta aggcaatgat cgacgcaccg ttgctaggag      360

tatttccttt tatgcccgaa cccgacttcg atgagctgtc ggcggcattc gagaaaaccc      420

tataaaaaat attgaaatta agttccaaat aaaccataat tcttttgcgg ttatttcggc      480

atgcaaaaca gtttgcaatg caaggggagg gcgaatgcgc cgcattctca taattcaaag      540

ccttgacgag tgaaaaggct caaacaaggc aattaatcaa cacaggagag gaaattatgg      600

ctttaatcac ttggacggca gatcaattcg gcaccaatgt cggttttgcc gacgatgaac      660

ataaagtttt gttcgacaaa ctcaataaac tttacgattt ggcgacaggc ggggcggaaa      720

gatccgcaat cggcgcgcaa ctcgatgatt taattgccta tgtcgtaggg catttcgcgc      780

atgaagaaaa agaaatggaa gcggccggtt ttgccggttt ggcggcacat aaagccgaac      840

acgaggcctt agtgggaact tgcgccgatc tacaaaaaaa attccatgcc ggcgaagccg      900

atgtaaccga tgaggtgggg caaatggtta agagttggtt ggaaagtcac attccgactt      960

tcgatagagg ctatgccgcc gttttgagtt aatcgatgaa aaaatgaatg gatgcttttt     1020

caactttttc aatatgaagt tagcattttt cagaaaatat ttcgatacgg ttgtagccta     1080

aattgacggt taaggttact gtcgatgaag actgttagcc cggaatagac tgtgaaaatg     1140

aagataattg aagcccgtga atagggaatc acgggctttt ttgtgcgcgt tagttttaag     1200

gcatcggcaa agctcttctg gcttaccgtt gcatcatggg ttaaaattct cgtttttaaa     1260

atagctatta taaaaacagg gaaattagtg aatgttgatt tgctttgaac cccggctaaa     1320

agtagctgat cagcgagaga aactttttct ctatttcatt gacttaagat agggcgcttg     1380

ccgttagaaa gccaaatcgt acaagccttg atcttaaaac aagctggcgc tgatgacgtt     1440

actaagaatt tatcaattgc atacaagagt ttctaactac caccgggagg ctgctccgtg     1500

aagaaaacca agcaactgtt cgcaagcctg ttcttgatgt ccttgggact agggaatgca     1560

caggccgaat atacactgaa tctgaggcaa ggcgtaaccc agatcagtca agaagtctac     1620

gatttgcata tgttgatttt atggatatgt gtgttaatcg gtattgctgt gttcggcaca     1680

atgttctact cgatatatca ccatcgtaaa tcgaaagggc atgtcgcatc tcaatttcat     1740

gagagcacca aagtcgaaat tatctggacg atcattccga ctttgattct gatcggtatg     1800

gcgattccgg ctacaaaaac gatgctcgat ttggacgatg ttcaagatgc ggatatgtcg     1860

atcaaagtca ccgggtggca atggaaatgg gaatatgaat acctggataa cggtattcac     1920

tttttcagta ccttggacga agccagtaat agagcgcgtc agctgggttc cggtatcgat     1980

ccgagatccg tg                                                         1992

