                         SEQUENCE LISTING

<110>  SynAffix B.V.
 
<120>  Modified glycoprotein, protein-conjugate and process for the 
       preparation thereof

<130>  P6047947PCT

<150>  EP 14165548.0
<151>  2014-04-23

<150>  EP 14165581.1
<151>  2014-04-23

<150>  EP 13188585.7
<151>  2013-10-14

<150>  EP 13188537.8
<151>  2013-10-14

<160>  24    

<170>  PatentIn version 3.3

<210>  1
<211>  39
<212>  DNA
<213>  artificial

<220>
<223>  primer sequence

<400>  1
cagcgacata tgtcgctgac cgcatgccct gaggagtcc                            39


<210>  2
<211>  36
<212>  DNA
<213>  artificial

<220>
<223>  primer sequence

<400>  2
gacacctcca aagttctgca cgtaaggtag gctaaa                               36


<210>  3
<211>  39
<212>  DNA
<213>  artificial

<220>
<223>  primer sequence

<400>  3
ctgatggatg gatccctagc tcggcgtccc gatgtccac                            39


<210>  4
<211>  36
<212>  DNA
<213>  artificial

<220>
<223>  primer sequence

<400>  4
ccttacgtgc agaactttgg aggtgtctct gctcta                               36


<210>  5
<211>  36
<212>  DNA
<213>  artificial

<220>
<223>  primer sequence

<400>  5
gacacctcca aaaaactgca cgtaaggtag gctaaa                               36


<210>  6
<211>  36
<212>  DNA
<213>  artificial

<220>
<223>  primer sequence

<400>  6
ccttacgtgc agttttttgg aggtgtctct gctcta                               36


<210>  7
<211>  36
<212>  DNA
<213>  artificial

<220>
<223>  primer sequence

<400>  7
gacacctcca aacatctgca cgtaaggtag gctaaa                               36


<210>  8
<211>  36
<212>  DNA
<213>  artificial

<220>
<223>  primer sequence

<400>  8
ccttacgtgc agatgtttgg aggtgtctct gctcta                               36


<210>  9
<211>  36
<212>  DNA
<213>  artificial

<220>
<223>  primer sequence

<400>  9
gacacctcca aaaatctgca cgtaaggtag gctaaa                               36


<210>  10
<211>  36
<212>  DNA
<213>  artificial

<220>
<223>  primer sequence

<400>  10
ccttacgtgc agatttttgg aggtgtctct gctcta                               36


<210>  11
<211>  36
<212>  DNA
<213>  artificial

<220>
<223>  primer sequence

<400>  11
gacacctcca aacacctgca cgtaaggtag gctaaa                               36


<210>  12
<211>  36
<212>  DNA
<213>  artificial

<220>
<223>  primer sequence

<400>  12
ccttacgtgc aggtgtttgg aggtgtctct gctcta                               36


<210>  13
<211>  36
<212>  DNA
<213>  artificial

<220>
<223>  primer sequence

<400>  13
gacacctcca aacgcctgca cgtaaggtag gctaaa                               36


<210>  14
<211>  36
<212>  DNA
<213>  artificial

<220>
<223>  primer sequence

<400>  14
ccttacgtgc aggcgtttgg aggtgtctct gctcta                               36


<210>  15
<211>  36
<212>  DNA
<213>  artificial

<220>
<223>  primer sequence

<400>  15
gacacctcca aagccctgca cgtaaggtag gctaaa                               36


<210>  16
<211>  36
<212>  DNA
<213>  artificial

<220>
<223>  primer sequence

<400>  16
ccttacgtgc agggctttgg aggtgtctct gctcta                               36


<210>  17
<211>  402
<212>  PRT
<213>  Bos taurus

<400>  17

Met Lys Phe Arg Glu Pro Leu Leu Gly Gly Ser Ala Ala Met Pro Gly 
1               5                   10                  15      


Ala Ser Leu Gln Arg Ala Cys Arg Leu Leu Val Ala Val Cys Ala Leu 
            20                  25                  30          


His Leu Gly Val Thr Leu Val Tyr Tyr Leu Ala Gly Arg Asp Leu Arg 
        35                  40                  45              


Arg Leu Pro Gln Leu Val Gly Val His Pro Pro Leu Gln Gly Ser Ser 
    50                  55                  60                  


His Gly Ala Ala Ala Ile Gly Gln Pro Ser Gly Glu Leu Arg Leu Arg 
65                  70                  75                  80  


Gly Val Ala Pro Pro Pro Pro Leu Gln Asn Ser Ser Lys Pro Arg Ser 
                85                  90                  95      


Arg Ala Pro Ser Asn Leu Asp Ala Tyr Ser His Pro Gly Pro Gly Pro 
            100                 105                 110         


Gly Pro Gly Ser Asn Leu Thr Ser Ala Pro Val Pro Ser Thr Thr Thr 
        115                 120                 125             


Arg Ser Leu Thr Ala Cys Pro Glu Glu Ser Pro Leu Leu Val Gly Pro 
    130                 135                 140                 


Met Leu Ile Glu Phe Asn Ile Pro Val Asp Leu Lys Leu Ile Glu Gln 
145                 150                 155                 160 


Gln Asn Pro Lys Val Lys Leu Gly Gly Arg Tyr Thr Pro Met Asp Cys 
                165                 170                 175     


Ile Ser Pro His Lys Val Ala Ile Ile Ile Leu Phe Arg Asn Arg Gln 
            180                 185                 190         


Glu His Leu Lys Tyr Trp Leu Tyr Tyr Leu His Pro Met Val Gln Arg 
        195                 200                 205             


Gln Gln Leu Asp Tyr Gly Ile Tyr Val Ile Asn Gln Ala Gly Glu Ser 
    210                 215                 220                 


Met Phe Asn Arg Ala Lys Leu Leu Asn Val Gly Phe Lys Glu Ala Leu 
225                 230                 235                 240 


Lys Asp Tyr Asp Tyr Asn Cys Phe Val Phe Ser Asp Val Asp Leu Ile 
                245                 250                 255     


Pro Met Asn Asp His Asn Thr Tyr Arg Cys Phe Ser Gln Pro Arg His 
            260                 265                 270         


Ile Ser Val Ala Met Asp Lys Phe Gly Phe Ser Leu Pro Tyr Val Gln 
        275                 280                 285             


Tyr Phe Gly Gly Val Ser Ala Leu Ser Lys Gln Gln Phe Leu Ser Ile 
    290                 295                 300                 


Asn Gly Phe Pro Asn Asn Tyr Trp Gly Trp Gly Gly Glu Asp Asp Asp 
305                 310                 315                 320 


Ile Tyr Asn Arg Leu Ala Phe Arg Gly Met Ser Val Ser Arg Pro Asn 
                325                 330                 335     


Ala Val Ile Gly Lys Cys Arg Met Ile Arg His Ser Arg Asp Lys Lys 
            340                 345                 350         


Asn Glu Pro Asn Pro Gln Arg Phe Asp Arg Ile Ala His Thr Lys Glu 
        355                 360                 365             


Thr Met Leu Ser Asp Gly Leu Asn Ser Leu Thr Tyr Met Val Leu Glu 
    370                 375                 380                 


Val Gln Arg Tyr Pro Leu Tyr Thr Lys Ile Thr Val Asp Ile Gly Thr 
385                 390                 395                 400 


Pro Ser 
        


<210>  18
<211>  402
<212>  PRT
<213>  artificial

<220>
<223>  bos taurus GalT Y289N

<400>  18

Met Lys Phe Arg Glu Pro Leu Leu Gly Gly Ser Ala Ala Met Pro Gly 
1               5                   10                  15      


Ala Ser Leu Gln Arg Ala Cys Arg Leu Leu Val Ala Val Cys Ala Leu 
            20                  25                  30          


His Leu Gly Val Thr Leu Val Tyr Tyr Leu Ala Gly Arg Asp Leu Arg 
        35                  40                  45              


Arg Leu Pro Gln Leu Val Gly Val His Pro Pro Leu Gln Gly Ser Ser 
    50                  55                  60                  


His Gly Ala Ala Ala Ile Gly Gln Pro Ser Gly Glu Leu Arg Leu Arg 
65                  70                  75                  80  


Gly Val Ala Pro Pro Pro Pro Leu Gln Asn Ser Ser Lys Pro Arg Ser 
                85                  90                  95      


Arg Ala Pro Ser Asn Leu Asp Ala Tyr Ser His Pro Gly Pro Gly Pro 
            100                 105                 110         


Gly Pro Gly Ser Asn Leu Thr Ser Ala Pro Val Pro Ser Thr Thr Thr 
        115                 120                 125             


Arg Ser Leu Thr Ala Cys Pro Glu Glu Ser Pro Leu Leu Val Gly Pro 
    130                 135                 140                 


Met Leu Ile Glu Phe Asn Ile Pro Val Asp Leu Lys Leu Ile Glu Gln 
145                 150                 155                 160 


Gln Asn Pro Lys Val Lys Leu Gly Gly Arg Tyr Thr Pro Met Asp Cys 
                165                 170                 175     


Ile Ser Pro His Lys Val Ala Ile Ile Ile Leu Phe Arg Asn Arg Gln 
            180                 185                 190         


Glu His Leu Lys Tyr Trp Leu Tyr Tyr Leu His Pro Met Val Gln Arg 
        195                 200                 205             


Gln Gln Leu Asp Tyr Gly Ile Tyr Val Ile Asn Gln Ala Gly Glu Ser 
    210                 215                 220                 


Met Phe Asn Arg Ala Lys Leu Leu Asn Val Gly Phe Lys Glu Ala Leu 
225                 230                 235                 240 


Lys Asp Tyr Asp Tyr Asn Cys Phe Val Phe Ser Asp Val Asp Leu Ile 
                245                 250                 255     


Pro Met Asn Asp His Asn Thr Tyr Arg Cys Phe Ser Gln Pro Arg His 
            260                 265                 270         


Ile Ser Val Ala Met Asp Lys Phe Gly Phe Ser Leu Pro Tyr Val Gln 
        275                 280                 285             


Asn Phe Gly Gly Val Ser Ala Leu Ser Lys Gln Gln Phe Leu Ser Ile 
    290                 295                 300                 


Asn Gly Phe Pro Asn Asn Tyr Trp Gly Trp Gly Gly Glu Asp Asp Asp 
305                 310                 315                 320 


Ile Tyr Asn Arg Leu Ala Phe Arg Gly Met Ser Val Ser Arg Pro Asn 
                325                 330                 335     


Ala Val Ile Gly Lys Cys Arg Met Ile Arg His Ser Arg Asp Lys Lys 
            340                 345                 350         


Asn Glu Pro Asn Pro Gln Arg Phe Asp Arg Ile Ala His Thr Lys Glu 
        355                 360                 365             


Thr Met Leu Ser Asp Gly Leu Asn Ser Leu Thr Tyr Met Val Leu Glu 
    370                 375                 380                 


Val Gln Arg Tyr Pro Leu Tyr Thr Lys Ile Thr Val Asp Ile Gly Thr 
385                 390                 395                 400 


Pro Ser 
        


<210>  19
<211>  402
<212>  PRT
<213>  artificial

<220>
<223>  bos taurus GalT Y289F

<400>  19

Met Lys Phe Arg Glu Pro Leu Leu Gly Gly Ser Ala Ala Met Pro Gly 
1               5                   10                  15      


Ala Ser Leu Gln Arg Ala Cys Arg Leu Leu Val Ala Val Cys Ala Leu 
            20                  25                  30          


His Leu Gly Val Thr Leu Val Tyr Tyr Leu Ala Gly Arg Asp Leu Arg 
        35                  40                  45              


Arg Leu Pro Gln Leu Val Gly Val His Pro Pro Leu Gln Gly Ser Ser 
    50                  55                  60                  


His Gly Ala Ala Ala Ile Gly Gln Pro Ser Gly Glu Leu Arg Leu Arg 
65                  70                  75                  80  


Gly Val Ala Pro Pro Pro Pro Leu Gln Asn Ser Ser Lys Pro Arg Ser 
                85                  90                  95      


Arg Ala Pro Ser Asn Leu Asp Ala Tyr Ser His Pro Gly Pro Gly Pro 
            100                 105                 110         


Gly Pro Gly Ser Asn Leu Thr Ser Ala Pro Val Pro Ser Thr Thr Thr 
        115                 120                 125             


Arg Ser Leu Thr Ala Cys Pro Glu Glu Ser Pro Leu Leu Val Gly Pro 
    130                 135                 140                 


Met Leu Ile Glu Phe Asn Ile Pro Val Asp Leu Lys Leu Ile Glu Gln 
145                 150                 155                 160 


Gln Asn Pro Lys Val Lys Leu Gly Gly Arg Tyr Thr Pro Met Asp Cys 
                165                 170                 175     


Ile Ser Pro His Lys Val Ala Ile Ile Ile Leu Phe Arg Asn Arg Gln 
            180                 185                 190         


Glu His Leu Lys Tyr Trp Leu Tyr Tyr Leu His Pro Met Val Gln Arg 
        195                 200                 205             


Gln Gln Leu Asp Tyr Gly Ile Tyr Val Ile Asn Gln Ala Gly Glu Ser 
    210                 215                 220                 


Met Phe Asn Arg Ala Lys Leu Leu Asn Val Gly Phe Lys Glu Ala Leu 
225                 230                 235                 240 


Lys Asp Tyr Asp Tyr Asn Cys Phe Val Phe Ser Asp Val Asp Leu Ile 
                245                 250                 255     


Pro Met Asn Asp His Asn Thr Tyr Arg Cys Phe Ser Gln Pro Arg His 
            260                 265                 270         


Ile Ser Val Ala Met Asp Lys Phe Gly Phe Ser Leu Pro Tyr Val Gln 
        275                 280                 285             


Phe Phe Gly Gly Val Ser Ala Leu Ser Lys Gln Gln Phe Leu Ser Ile 
    290                 295                 300                 


Asn Gly Phe Pro Asn Asn Tyr Trp Gly Trp Gly Gly Glu Asp Asp Asp 
305                 310                 315                 320 


Ile Tyr Asn Arg Leu Ala Phe Arg Gly Met Ser Val Ser Arg Pro Asn 
                325                 330                 335     


Ala Val Ile Gly Lys Cys Arg Met Ile Arg His Ser Arg Asp Lys Lys 
            340                 345                 350         


Asn Glu Pro Asn Pro Gln Arg Phe Asp Arg Ile Ala His Thr Lys Glu 
        355                 360                 365             


Thr Met Leu Ser Asp Gly Leu Asn Ser Leu Thr Tyr Met Val Leu Glu 
    370                 375                 380                 


Val Gln Arg Tyr Pro Leu Tyr Thr Lys Ile Thr Val Asp Ile Gly Thr 
385                 390                 395                 400 


Pro Ser 
        


<210>  20
<211>  402
<212>  PRT
<213>  artificial

<220>
<223>  bos taurus GalT Y289M

<400>  20

Met Lys Phe Arg Glu Pro Leu Leu Gly Gly Ser Ala Ala Met Pro Gly 
1               5                   10                  15      


Ala Ser Leu Gln Arg Ala Cys Arg Leu Leu Val Ala Val Cys Ala Leu 
            20                  25                  30          


His Leu Gly Val Thr Leu Val Tyr Tyr Leu Ala Gly Arg Asp Leu Arg 
        35                  40                  45              


Arg Leu Pro Gln Leu Val Gly Val His Pro Pro Leu Gln Gly Ser Ser 
    50                  55                  60                  


His Gly Ala Ala Ala Ile Gly Gln Pro Ser Gly Glu Leu Arg Leu Arg 
65                  70                  75                  80  


Gly Val Ala Pro Pro Pro Pro Leu Gln Asn Ser Ser Lys Pro Arg Ser 
                85                  90                  95      


Arg Ala Pro Ser Asn Leu Asp Ala Tyr Ser His Pro Gly Pro Gly Pro 
            100                 105                 110         


Gly Pro Gly Ser Asn Leu Thr Ser Ala Pro Val Pro Ser Thr Thr Thr 
        115                 120                 125             


Arg Ser Leu Thr Ala Cys Pro Glu Glu Ser Pro Leu Leu Val Gly Pro 
    130                 135                 140                 


Met Leu Ile Glu Phe Asn Ile Pro Val Asp Leu Lys Leu Ile Glu Gln 
145                 150                 155                 160 


Gln Asn Pro Lys Val Lys Leu Gly Gly Arg Tyr Thr Pro Met Asp Cys 
                165                 170                 175     


Ile Ser Pro His Lys Val Ala Ile Ile Ile Leu Phe Arg Asn Arg Gln 
            180                 185                 190         


Glu His Leu Lys Tyr Trp Leu Tyr Tyr Leu His Pro Met Val Gln Arg 
        195                 200                 205             


Gln Gln Leu Asp Tyr Gly Ile Tyr Val Ile Asn Gln Ala Gly Glu Ser 
    210                 215                 220                 


Met Phe Asn Arg Ala Lys Leu Leu Asn Val Gly Phe Lys Glu Ala Leu 
225                 230                 235                 240 


Lys Asp Tyr Asp Tyr Asn Cys Phe Val Phe Ser Asp Val Asp Leu Ile 
                245                 250                 255     


Pro Met Asn Asp His Asn Thr Tyr Arg Cys Phe Ser Gln Pro Arg His 
            260                 265                 270         


Ile Ser Val Ala Met Asp Lys Phe Gly Phe Ser Leu Pro Tyr Val Gln 
        275                 280                 285             


Met Phe Gly Gly Val Ser Ala Leu Ser Lys Gln Gln Phe Leu Ser Ile 
    290                 295                 300                 


Asn Gly Phe Pro Asn Asn Tyr Trp Gly Trp Gly Gly Glu Asp Asp Asp 
305                 310                 315                 320 


Ile Tyr Asn Arg Leu Ala Phe Arg Gly Met Ser Val Ser Arg Pro Asn 
                325                 330                 335     


Ala Val Ile Gly Lys Cys Arg Met Ile Arg His Ser Arg Asp Lys Lys 
            340                 345                 350         


Asn Glu Pro Asn Pro Gln Arg Phe Asp Arg Ile Ala His Thr Lys Glu 
        355                 360                 365             


Thr Met Leu Ser Asp Gly Leu Asn Ser Leu Thr Tyr Met Val Leu Glu 
    370                 375                 380                 


Val Gln Arg Tyr Pro Leu Tyr Thr Lys Ile Thr Val Asp Ile Gly Thr 
385                 390                 395                 400 


Pro Ser 
        


<210>  21
<211>  402
<212>  PRT
<213>  artificial

<220>
<223>  bos taurus GalT Y289I

<400>  21

Met Lys Phe Arg Glu Pro Leu Leu Gly Gly Ser Ala Ala Met Pro Gly 
1               5                   10                  15      


Ala Ser Leu Gln Arg Ala Cys Arg Leu Leu Val Ala Val Cys Ala Leu 
            20                  25                  30          


His Leu Gly Val Thr Leu Val Tyr Tyr Leu Ala Gly Arg Asp Leu Arg 
        35                  40                  45              


Arg Leu Pro Gln Leu Val Gly Val His Pro Pro Leu Gln Gly Ser Ser 
    50                  55                  60                  


His Gly Ala Ala Ala Ile Gly Gln Pro Ser Gly Glu Leu Arg Leu Arg 
65                  70                  75                  80  


Gly Val Ala Pro Pro Pro Pro Leu Gln Asn Ser Ser Lys Pro Arg Ser 
                85                  90                  95      


Arg Ala Pro Ser Asn Leu Asp Ala Tyr Ser His Pro Gly Pro Gly Pro 
            100                 105                 110         


Gly Pro Gly Ser Asn Leu Thr Ser Ala Pro Val Pro Ser Thr Thr Thr 
        115                 120                 125             


Arg Ser Leu Thr Ala Cys Pro Glu Glu Ser Pro Leu Leu Val Gly Pro 
    130                 135                 140                 


Met Leu Ile Glu Phe Asn Ile Pro Val Asp Leu Lys Leu Ile Glu Gln 
145                 150                 155                 160 


Gln Asn Pro Lys Val Lys Leu Gly Gly Arg Tyr Thr Pro Met Asp Cys 
                165                 170                 175     


Ile Ser Pro His Lys Val Ala Ile Ile Ile Leu Phe Arg Asn Arg Gln 
            180                 185                 190         


Glu His Leu Lys Tyr Trp Leu Tyr Tyr Leu His Pro Met Val Gln Arg 
        195                 200                 205             


Gln Gln Leu Asp Tyr Gly Ile Tyr Val Ile Asn Gln Ala Gly Glu Ser 
    210                 215                 220                 


Met Phe Asn Arg Ala Lys Leu Leu Asn Val Gly Phe Lys Glu Ala Leu 
225                 230                 235                 240 


Lys Asp Tyr Asp Tyr Asn Cys Phe Val Phe Ser Asp Val Asp Leu Ile 
                245                 250                 255     


Pro Met Asn Asp His Asn Thr Tyr Arg Cys Phe Ser Gln Pro Arg His 
            260                 265                 270         


Ile Ser Val Ala Met Asp Lys Phe Gly Phe Ser Leu Pro Tyr Val Gln 
        275                 280                 285             


Ile Phe Gly Gly Val Ser Ala Leu Ser Lys Gln Gln Phe Leu Ser Ile 
    290                 295                 300                 


Asn Gly Phe Pro Asn Asn Tyr Trp Gly Trp Gly Gly Glu Asp Asp Asp 
305                 310                 315                 320 


Ile Tyr Asn Arg Leu Ala Phe Arg Gly Met Ser Val Ser Arg Pro Asn 
                325                 330                 335     


Ala Val Ile Gly Lys Cys Arg Met Ile Arg His Ser Arg Asp Lys Lys 
            340                 345                 350         


Asn Glu Pro Asn Pro Gln Arg Phe Asp Arg Ile Ala His Thr Lys Glu 
        355                 360                 365             


Thr Met Leu Ser Asp Gly Leu Asn Ser Leu Thr Tyr Met Val Leu Glu 
    370                 375                 380                 


Val Gln Arg Tyr Pro Leu Tyr Thr Lys Ile Thr Val Asp Ile Gly Thr 
385                 390                 395                 400 


Pro Ser 
        


<210>  22
<211>  402
<212>  PRT
<213>  artificial

<220>
<223>  bos taurus GalT Y289V

<400>  22

Met Lys Phe Arg Glu Pro Leu Leu Gly Gly Ser Ala Ala Met Pro Gly 
1               5                   10                  15      


Ala Ser Leu Gln Arg Ala Cys Arg Leu Leu Val Ala Val Cys Ala Leu 
            20                  25                  30          


His Leu Gly Val Thr Leu Val Tyr Tyr Leu Ala Gly Arg Asp Leu Arg 
        35                  40                  45              


Arg Leu Pro Gln Leu Val Gly Val His Pro Pro Leu Gln Gly Ser Ser 
    50                  55                  60                  


His Gly Ala Ala Ala Ile Gly Gln Pro Ser Gly Glu Leu Arg Leu Arg 
65                  70                  75                  80  


Gly Val Ala Pro Pro Pro Pro Leu Gln Asn Ser Ser Lys Pro Arg Ser 
                85                  90                  95      


Arg Ala Pro Ser Asn Leu Asp Ala Tyr Ser His Pro Gly Pro Gly Pro 
            100                 105                 110         


Gly Pro Gly Ser Asn Leu Thr Ser Ala Pro Val Pro Ser Thr Thr Thr 
        115                 120                 125             


Arg Ser Leu Thr Ala Cys Pro Glu Glu Ser Pro Leu Leu Val Gly Pro 
    130                 135                 140                 


Met Leu Ile Glu Phe Asn Ile Pro Val Asp Leu Lys Leu Ile Glu Gln 
145                 150                 155                 160 


Gln Asn Pro Lys Val Lys Leu Gly Gly Arg Tyr Thr Pro Met Asp Cys 
                165                 170                 175     


Ile Ser Pro His Lys Val Ala Ile Ile Ile Leu Phe Arg Asn Arg Gln 
            180                 185                 190         


Glu His Leu Lys Tyr Trp Leu Tyr Tyr Leu His Pro Met Val Gln Arg 
        195                 200                 205             


Gln Gln Leu Asp Tyr Gly Ile Tyr Val Ile Asn Gln Ala Gly Glu Ser 
    210                 215                 220                 


Met Phe Asn Arg Ala Lys Leu Leu Asn Val Gly Phe Lys Glu Ala Leu 
225                 230                 235                 240 


Lys Asp Tyr Asp Tyr Asn Cys Phe Val Phe Ser Asp Val Asp Leu Ile 
                245                 250                 255     


Pro Met Asn Asp His Asn Thr Tyr Arg Cys Phe Ser Gln Pro Arg His 
            260                 265                 270         


Ile Ser Val Ala Met Asp Lys Phe Gly Phe Ser Leu Pro Tyr Val Gln 
        275                 280                 285             


Val Phe Gly Gly Val Ser Ala Leu Ser Lys Gln Gln Phe Leu Ser Ile 
    290                 295                 300                 


Asn Gly Phe Pro Asn Asn Tyr Trp Gly Trp Gly Gly Glu Asp Asp Asp 
305                 310                 315                 320 


Ile Tyr Asn Arg Leu Ala Phe Arg Gly Met Ser Val Ser Arg Pro Asn 
                325                 330                 335     


Ala Val Ile Gly Lys Cys Arg Met Ile Arg His Ser Arg Asp Lys Lys 
            340                 345                 350         


Asn Glu Pro Asn Pro Gln Arg Phe Asp Arg Ile Ala His Thr Lys Glu 
        355                 360                 365             


Thr Met Leu Ser Asp Gly Leu Asn Ser Leu Thr Tyr Met Val Leu Glu 
    370                 375                 380                 


Val Gln Arg Tyr Pro Leu Tyr Thr Lys Ile Thr Val Asp Ile Gly Thr 
385                 390                 395                 400 


Pro Ser 
        


<210>  23
<211>  402
<212>  PRT
<213>  artificial

<220>
<223>  bos taurus GalT Y289A

<400>  23

Met Lys Phe Arg Glu Pro Leu Leu Gly Gly Ser Ala Ala Met Pro Gly 
1               5                   10                  15      


Ala Ser Leu Gln Arg Ala Cys Arg Leu Leu Val Ala Val Cys Ala Leu 
            20                  25                  30          


His Leu Gly Val Thr Leu Val Tyr Tyr Leu Ala Gly Arg Asp Leu Arg 
        35                  40                  45              


Arg Leu Pro Gln Leu Val Gly Val His Pro Pro Leu Gln Gly Ser Ser 
    50                  55                  60                  


His Gly Ala Ala Ala Ile Gly Gln Pro Ser Gly Glu Leu Arg Leu Arg 
65                  70                  75                  80  


Gly Val Ala Pro Pro Pro Pro Leu Gln Asn Ser Ser Lys Pro Arg Ser 
                85                  90                  95      


Arg Ala Pro Ser Asn Leu Asp Ala Tyr Ser His Pro Gly Pro Gly Pro 
            100                 105                 110         


Gly Pro Gly Ser Asn Leu Thr Ser Ala Pro Val Pro Ser Thr Thr Thr 
        115                 120                 125             


Arg Ser Leu Thr Ala Cys Pro Glu Glu Ser Pro Leu Leu Val Gly Pro 
    130                 135                 140                 


Met Leu Ile Glu Phe Asn Ile Pro Val Asp Leu Lys Leu Ile Glu Gln 
145                 150                 155                 160 


Gln Asn Pro Lys Val Lys Leu Gly Gly Arg Tyr Thr Pro Met Asp Cys 
                165                 170                 175     


Ile Ser Pro His Lys Val Ala Ile Ile Ile Leu Phe Arg Asn Arg Gln 
            180                 185                 190         


Glu His Leu Lys Tyr Trp Leu Tyr Tyr Leu His Pro Met Val Gln Arg 
        195                 200                 205             


Gln Gln Leu Asp Tyr Gly Ile Tyr Val Ile Asn Gln Ala Gly Glu Ser 
    210                 215                 220                 


Met Phe Asn Arg Ala Lys Leu Leu Asn Val Gly Phe Lys Glu Ala Leu 
225                 230                 235                 240 


Lys Asp Tyr Asp Tyr Asn Cys Phe Val Phe Ser Asp Val Asp Leu Ile 
                245                 250                 255     


Pro Met Asn Asp His Asn Thr Tyr Arg Cys Phe Ser Gln Pro Arg His 
            260                 265                 270         


Ile Ser Val Ala Met Asp Lys Phe Gly Phe Ser Leu Pro Tyr Val Gln 
        275                 280                 285             


Ala Phe Gly Gly Val Ser Ala Leu Ser Lys Gln Gln Phe Leu Ser Ile 
    290                 295                 300                 


Asn Gly Phe Pro Asn Asn Tyr Trp Gly Trp Gly Gly Glu Asp Asp Asp 
305                 310                 315                 320 


Ile Tyr Asn Arg Leu Ala Phe Arg Gly Met Ser Val Ser Arg Pro Asn 
                325                 330                 335     


Ala Val Ile Gly Lys Cys Arg Met Ile Arg His Ser Arg Asp Lys Lys 
            340                 345                 350         


Asn Glu Pro Asn Pro Gln Arg Phe Asp Arg Ile Ala His Thr Lys Glu 
        355                 360                 365             


Thr Met Leu Ser Asp Gly Leu Asn Ser Leu Thr Tyr Met Val Leu Glu 
    370                 375                 380                 


Val Gln Arg Tyr Pro Leu Tyr Thr Lys Ile Thr Val Asp Ile Gly Thr 
385                 390                 395                 400 


Pro Ser 
        


<210>  24
<211>  402
<212>  PRT
<213>  artificial

<220>
<223>  bos taurus GalT Y289G

<400>  24

Met Lys Phe Arg Glu Pro Leu Leu Gly Gly Ser Ala Ala Met Pro Gly 
1               5                   10                  15      


Ala Ser Leu Gln Arg Ala Cys Arg Leu Leu Val Ala Val Cys Ala Leu 
            20                  25                  30          


His Leu Gly Val Thr Leu Val Tyr Tyr Leu Ala Gly Arg Asp Leu Arg 
        35                  40                  45              


Arg Leu Pro Gln Leu Val Gly Val His Pro Pro Leu Gln Gly Ser Ser 
    50                  55                  60                  


His Gly Ala Ala Ala Ile Gly Gln Pro Ser Gly Glu Leu Arg Leu Arg 
65                  70                  75                  80  


Gly Val Ala Pro Pro Pro Pro Leu Gln Asn Ser Ser Lys Pro Arg Ser 
                85                  90                  95      


Arg Ala Pro Ser Asn Leu Asp Ala Tyr Ser His Pro Gly Pro Gly Pro 
            100                 105                 110         


Gly Pro Gly Ser Asn Leu Thr Ser Ala Pro Val Pro Ser Thr Thr Thr 
        115                 120                 125             


Arg Ser Leu Thr Ala Cys Pro Glu Glu Ser Pro Leu Leu Val Gly Pro 
    130                 135                 140                 


Met Leu Ile Glu Phe Asn Ile Pro Val Asp Leu Lys Leu Ile Glu Gln 
145                 150                 155                 160 


Gln Asn Pro Lys Val Lys Leu Gly Gly Arg Tyr Thr Pro Met Asp Cys 
                165                 170                 175     


Ile Ser Pro His Lys Val Ala Ile Ile Ile Leu Phe Arg Asn Arg Gln 
            180                 185                 190         


Glu His Leu Lys Tyr Trp Leu Tyr Tyr Leu His Pro Met Val Gln Arg 
        195                 200                 205             


Gln Gln Leu Asp Tyr Gly Ile Tyr Val Ile Asn Gln Ala Gly Glu Ser 
    210                 215                 220                 


Met Phe Asn Arg Ala Lys Leu Leu Asn Val Gly Phe Lys Glu Ala Leu 
225                 230                 235                 240 


Lys Asp Tyr Asp Tyr Asn Cys Phe Val Phe Ser Asp Val Asp Leu Ile 
                245                 250                 255     


Pro Met Asn Asp His Asn Thr Tyr Arg Cys Phe Ser Gln Pro Arg His 
            260                 265                 270         


Ile Ser Val Ala Met Asp Lys Phe Gly Phe Ser Leu Pro Tyr Val Gln 
        275                 280                 285             


Gly Phe Gly Gly Val Ser Ala Leu Ser Lys Gln Gln Phe Leu Ser Ile 
    290                 295                 300                 


Asn Gly Phe Pro Asn Asn Tyr Trp Gly Trp Gly Gly Glu Asp Asp Asp 
305                 310                 315                 320 


Ile Tyr Asn Arg Leu Ala Phe Arg Gly Met Ser Val Ser Arg Pro Asn 
                325                 330                 335     


Ala Val Ile Gly Lys Cys Arg Met Ile Arg His Ser Arg Asp Lys Lys 
            340                 345                 350         


Asn Glu Pro Asn Pro Gln Arg Phe Asp Arg Ile Ala His Thr Lys Glu 
        355                 360                 365             


Thr Met Leu Ser Asp Gly Leu Asn Ser Leu Thr Tyr Met Val Leu Glu 
    370                 375                 380                 


Val Gln Arg Tyr Pro Leu Tyr Thr Lys Ile Thr Val Asp Ile Gly Thr 
385                 390                 395                 400 


Pro Ser 
        


