                         SEQUENCE LISTING

<110>  Joint Center for Biosciences
       Choe, Senyon
       Kuo, Mario Meng-Chiang
       Jung, Jae Woo
       Lee, Dong Kun
 
<120>  BIOACTIVE RECOMBINANT BMP-9 PROTEIN, MB109, EXPRESSED AND 
       ISOLATED FROM BACTERIA

<130>  00059-003WO1

<140>  Not yet assigned
<141>  2014-10-16

<150>  US 61/892,096
<151>  2013-10-17

<160>  36    

<170>  PatentIn version 3.5

<210>  1
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MB109 coding sequence


<220>
<221>  CDS
<222>  (1)..(336)

<400>  1
atg tca gca ggt gct ggt tcg cat tgt caa aaa acc agt ctg cgc gtt         48
Met Ser Ala Gly Ala Gly Ser His Cys Gln Lys Thr Ser Leu Arg Val           
1               5                   10                  15                

aat ttt gaa gat att ggt tgg gac tcc tgg att atc gcg ccg aaa gaa         96
Asn Phe Glu Asp Ile Gly Trp Asp Ser Trp Ile Ile Ala Pro Lys Glu           
            20                  25                  30                    

tat gaa gcc tac gaa tgc aaa ggt ggc tgc ttt ttc ccg ctg gca gat        144
Tyr Glu Ala Tyr Glu Cys Lys Gly Gly Cys Phe Phe Pro Leu Ala Asp           
        35                  40                  45                        

gac gtc acg ccg acc aaa cat gct att gtc cag acg ctg gtg cac ctg        192
Asp Val Thr Pro Thr Lys His Ala Ile Val Gln Thr Leu Val His Leu           
    50                  55                  60                            

aaa ttc ccg acc aaa gtg ggc aaa gcg tgc tgt gtt ccg acc aaa ctg        240
Lys Phe Pro Thr Lys Val Gly Lys Ala Cys Cys Val Pro Thr Lys Leu           
65                  70                  75                  80            

tct ccg atc agt gtg ctg tat aaa gat gac atg ggt gtc ccg acc ctg        288
Ser Pro Ile Ser Val Leu Tyr Lys Asp Asp Met Gly Val Pro Thr Leu           
                85                  90                  95                

aaa tac cat tac gaa ggc atg agt gtg gcg gaa tgt ggc tgt cgc taa        336
Lys Tyr His Tyr Glu Gly Met Ser Val Ala Glu Cys Gly Cys Arg               
            100                 105                 110                   


<210>  2
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  2

Met Ser Ala Gly Ala Gly Ser His Cys Gln Lys Thr Ser Leu Arg Val 
1               5                   10                  15      


Asn Phe Glu Asp Ile Gly Trp Asp Ser Trp Ile Ile Ala Pro Lys Glu 
            20                  25                  30          


Tyr Glu Ala Tyr Glu Cys Lys Gly Gly Cys Phe Phe Pro Leu Ala Asp 
        35                  40                  45              


Asp Val Thr Pro Thr Lys His Ala Ile Val Gln Thr Leu Val His Leu 
    50                  55                  60                  


Lys Phe Pro Thr Lys Val Gly Lys Ala Cys Cys Val Pro Thr Lys Leu 
65                  70                  75                  80  


Ser Pro Ile Ser Val Leu Tyr Lys Asp Asp Met Gly Val Pro Thr Leu 
                85                  90                  95      


Lys Tyr His Tyr Glu Gly Met Ser Val Ala Glu Cys Gly Cys Arg 
            100                 105                 110     


<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Beta Actin Forward Primer

<400>  3
ctaaggccaa ccgtgaaaag                                                   20


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Beta Actin Reverse Primer

<400>  4
accagaggca tacagggaca                                                   20


<210>  5
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p21 Forward Primer

<400>  5
ccagcctctg gcattagaat ta                                                22


<210>  6
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p21 Reverse Primer

<400>  6
cgggatgagg aggctttaaa ta                                                22


<210>  7
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Survivin Forward Primer

<400>  7
gatgacgacc ccatagagga ac                                                22


<210>  8
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Survivin Reverse Primer

<400>  8
gggttaattc ttcaaactgc ttct                                              24


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBx Forward Primer

<400>  9
acggggcgca cctctcttta                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBx Reverse Primer

<400>  10
tgcctacagc ctcctagtac                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AP2 Forward Primer

<400>  11
catgtgcaga aatgggatgg                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AP2 Reverse Primer

<400>  12
aacttcagtc caggtcaacg                                                   20


<210>  13
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cyclophilin Forward Primer

<400>  13
cgaggaaaac cgtgtactat tag                                               23


<210>  14
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cyclophilin Reverse Primer

<400>  14
tgctgtcttt gggaccttg                                                    19


<210>  15
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C/EBP1-alpha Forward Primer

<400>  15
tggacaagag caacgag                                                      17


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C/EBP1-alpha Reverse Primer

<400>  16
tcattgtcac tggtcagctc                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PPAR gamma Forward Primer

<400>  17
gagcccaagt ttgagtttgc                                                   20


<210>  18
<211>  22
<212>  DNA
<213>  ARtificial Sequence

<220>
<223>  PPAR gamma Reverse Primer

<400>  18
gcaggttgtc ttgaatgtct tc                                                22


<210>  19
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD137 Forward Primer

<400>  19
cctgtgataa ctgtcagcct g                                                 21


<210>  20
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD137 Reverse Primer

<400>  20
tcttgaacct gaaatagcct gc                                                22


<210>  21
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CIDEA Forward Primer

<400>  21
atcacaactg gcctggttac g                                                 21


<210>  22
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CIDEA Reverse Primer

<400>  22
tactacccgg tgtccatttc t                                                 21


<210>  23
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cyclophilin Forward Primer

<400>  23
cagacgccac tgtcgcttt                                                    19


<210>  24
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cyclophilin Reverse Primer

<400>  24
tgtctttgga actttgtctg caa                                               23


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FAS Forward Primer

<400>  25
cccctctgtt aattggctcc                                                   20


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FAS Reverse Primer

<400>  26
ttgtggaagt gcaggttagg                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GLUT4 Forward Primer

<400>  27
gattctgctg cccttctgtc                                                   20


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GLUT4 Reverse Primer

<400>  28
attggacgct ctctctccaa                                                   20


<210>  29
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Leptin Forward Primer

<400>  29
gtgcctatcc agaaagtcca g                                                 21


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Leptin Reverse Primer

<400>  30
tgaagcccag gaatgaagtc                                                   20


<210>  31
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PEPCK Forward Primer

<400>  31
ccatcccaac tcgagattct g                                                 21


<210>  32
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PEPCK Reverse Primer

<400>  32
ctgagggctt catagacaag g                                                 21


<210>  33
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Resistin Forward Primer

<400>  33
tccttttctt ccttgtccct g                                                 21


<210>  34
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Resistin Reverse Primer

<400>  34
gactgtccag caatttaagc c                                                 21


<210>  35
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TMEM26 Forward Primer

<400>  35
gcaccatcac tagagaccaa c                                                 21


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TMEM26 Reverse Primer

<400>  36
acaagaatgc cagagaccag                                                   20


