                         SEQUENCE LISTING

<110>  The University Court of the University of Edinburgh
 
<120>  Proteins with Diagnostic and Terapeutic Uses

<130>  P209986WO

<150>  GB 1318170.6
<151>  2013-10-14

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  104
<212>  PRT
<213>  Streptococcus pneumoniae

<400>  1

Ala Thr Glu Asn Glu Gly Ser Thr Gln Ala Ala Thr Ser Ser Asn Met 
1               5                   10                  15      


Ala Lys Thr Glu His Arg Lys Ala Ala Lys Gln Val Val Asp Glu Tyr 
            20                  25                  30          


Ile Glu Lys Met Leu Arg Glu Ile Gln Leu Asp Arg Arg Lys His Thr 
        35                  40                  45              


Gln Asn Val Ala Leu Asn Ile Lys Leu Ser Ala Ile Lys Thr Lys Tyr 
    50                  55                  60                  


Leu Arg Glu Leu Asn Val Leu Glu Glu Lys Ser Lys Asp Glu Leu Pro 
65                  70                  75                  80  


Ser Glu Ile Lys Ala Lys Leu Asp Ala Ala Phe Glu Lys Phe Lys Lys 
                85                  90                  95      


Asp Thr Leu Lys Pro Gly Glu Lys 
            100                 


<210>  2
<211>  79
<212>  PRT
<213>  Streptococcus pneumoniae

<400>  2

Lys Gln Val Val Asp Glu Tyr Ile Glu Lys Met Leu Arg Glu Ile Gln 
1               5                   10                  15      


Leu Asp Arg Arg Lys His Thr Gln Asn Val Ala Leu Asn Ile Lys Leu 
            20                  25                  30          


Ser Ala Ile Lys Thr Lys Tyr Leu Arg Glu Leu Asn Val Leu Glu Glu 
        35                  40                  45              


Lys Ser Lys Asp Glu Leu Pro Ser Glu Ile Lys Ala Lys Leu Asp Ala 
    50                  55                  60                  


Ala Phe Glu Lys Phe Lys Lys Asp Thr Leu Lys Pro Gly Glu Lys 
65                  70                  75                  


<210>  3
<211>  2725
<212>  DNA
<213>  Streptococcus pneumoniae


<220>
<221>  misc_feature
<222>  (2260)..(2260)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (2723)..(2723)
<223>  n is a, c, g, or t

<400>  3
atgcttgtca ataatcacaa atatgtagat catatcttgt ttaggacagt aaaacatcct       60

aattactttt taaatattct tcctgagttg attggcttga ccttgttgag tcatgcttat      120

gtgacttttg ttttagtttt tccagtttat gcagttattt tgtatcgacg aatagctgaa      180

gaggaaaagc tattacatga agttataatc ccaaatggaa gcataaagag ataaatacaa      240

aattcgattt atatacagtt catattgaag taatatagta aggttaaaga aaaaatatag      300

aaggaaataa acatgtttgc atcaaaaagc gaaagaaaag tacattattc aattcgtaaa      360

tttagtattg gagtagctag tgtagctgtt gccagtcttg ttatgggaag tgtggttcat      420

gcgacagaga acgagggaag tacccaagca gccacttctt ctaatatggc aaagacagaa      480

cataggaaag ctgctaaaca agtcgtcgat gaatatatag aaaaaatgtt gagggagatt      540

caactagata gaagaaaaca tacccaaaat gtcgccttaa acataaagtt gagcgcaatt      600

aaaacgaagt atttgcgtga attaaatgtt ttagaagaga agtcgaaaga tgagttgccg      660

tcagaaataa aagcaaagtt agacgcagct tttgagaagt ttaaaaaaga tacattgaaa      720

ccaggagaaa aggtagcaga agctaagaag aaggttgaag aagctaagaa aaaagccgag      780

gatcaaaaag aagaagatcg tcgtaactac ccaaccaata cttacaaaac gcttgaactt      840

gaaattgctg agttcgatgt gaaagttaaa gaagcggagc ttgaactagt aaaagaggaa      900

gctaaagaat ctcgaaacga gggcacaatt aagcaagcaa aagagaaagt tgagagtaaa      960

aaagctgagg ctacaaggtt agaaaacatc aagacagatc gtaaaaaagc agaagaagaa     1020

gctaaacgaa aagcagatgg taagttgaag gaagctaatg tagcgacttc agatcaaggt     1080

aaaccaaagg ggcgggcaaa acgaggagtt cctggagagc tagcaacacc tgataaaaaa     1140

gaaaatgatg cgaagtcttc agattctagc gtaggtgaag aaactcttcc aagctcatcc     1200

ctgaaatcag gaaaaaaggt agcagaagct gagaagaagg ttgaagaagc tgagaaaaaa     1260

gccaaggatc aaaaagaaga agatcgccgt aactacccaa ccaatactta caaaacgctt     1320

gaccttgaaa ttgctgagtc cgatgtgaaa gttaaagaag cggagcttga actagtaaaa     1380

gaggaagcta aggaacctcg agacgaggaa aaaattaagc aagcaaaagc gaaagttgag     1440

agtaaaaaag ctgaggctac aaggttagaa aacatcaaga cagatcgtaa aaaagcagaa     1500

gaagaagcta aacgaaaagc agcagaagaa gataaagtta aagaaaaacc agctgaacaa     1560

ccacaaccag cgccggctac tcaaccagaa aaaccagctc caaaaccaga gaagccagct     1620

gaacaaccaa aagcagaaaa aacagatgat caacaagctg aagaagacta tgctcgtaga     1680

tcagaagaag aatataatcg cttgactcaa cagcaaccgc caaaaactga aaaaccagca     1740

caaccatcta ctccaaaaac aggctggaaa caagaaaacg gtatgtggta cttctacaat     1800

actgatggtt caatggcaac aggatggctc caaaacaacg gttcatggta ctatctaaac     1860

gctaatggtg ctatggcgac aggatggctc caaaacaatg gttcatggta ctatctaaac     1920

gctaatggtt caatggcaac aggatggctc caaaacaatg gttcatggta ctacctaaac     1980

gctaatggtg ctatggcgac aggatggctc caatacaatg gttcatggta ctacctaaac     2040

agcaatggcg ctatggcgac aggatggctc caatacaatg gctcatggta ctacctcaac     2100

gctaatggtg atatggcgac aggatggctc caaaacaacg gttcatggta ctacctcaac     2160

gctaatggtg atatggcgac aggatggctc caatacaacg gttcatggta ttacctcaac     2220

gctaatggtg atatggcgac aggttgggtg aaagatggan atacctggta ctatcttaaa     2280

gcatcaggtg ctatgaaagc aagccaatgg ttcaaagtat cagataaatg gtactatgtc     2340

aatggctcag gtgcccttgc agtcaacaca actgtagatg gctatggagt caatgccaat     2400

ggtgaatggg taaactaaac ctaatataac tagttaatac tgacttcctg taagaacttt     2460

ttaaagtatt ccctacaaat accatatcct ttcagtagat aatataccct tgtaggaagt     2520

ttagattaaa aaataactct gtaatctcta gccggattta tagcgctaga gactacggag     2580

tttttttgat gaggaaagaa tggcggcatt caagagactc tttaagagag ttacgggttt     2640

taaactatta agccttctcc aattgcaaga gggcttcaat ctctgctagg gtgctagctt     2700

gcgaaatggc tccacggagt ttngc                                           2725


<210>  4
<211>  701
<212>  PRT
<213>  Streptococcus pneumoniae


<220>
<221>  misc_feature
<222>  (650)..(650)
<223>  Xaa can be any naturally occurring amino acid

<400>  4

Met Phe Ala Ser Lys Ser Glu Arg Lys Val His Tyr Ser Ile Arg Lys 
1               5                   10                  15      


Phe Ser Ile Gly Val Ala Ser Val Ala Val Ala Ser Leu Val Met Gly 
            20                  25                  30          


Ser Val Val His Ala Thr Glu Asn Glu Gly Ser Thr Gln Ala Ala Thr 
        35                  40                  45              


Ser Ser Asn Met Ala Lys Thr Glu His Arg Lys Ala Ala Lys Gln Val 
    50                  55                  60                  


Val Asp Glu Tyr Ile Glu Lys Met Leu Arg Glu Ile Gln Leu Asp Arg 
65                  70                  75                  80  


Arg Lys His Thr Gln Asn Val Ala Leu Asn Ile Lys Leu Ser Ala Ile 
                85                  90                  95      


Lys Thr Lys Tyr Leu Arg Glu Leu Asn Val Leu Glu Glu Lys Ser Lys 
            100                 105                 110         


Asp Glu Leu Pro Ser Glu Ile Lys Ala Lys Leu Asp Ala Ala Phe Glu 
        115                 120                 125             


Lys Phe Lys Lys Asp Thr Leu Lys Pro Gly Glu Lys Val Ala Glu Ala 
    130                 135                 140                 


Lys Lys Lys Val Glu Glu Ala Lys Lys Lys Ala Glu Asp Gln Lys Glu 
145                 150                 155                 160 


Glu Asp Arg Arg Asn Tyr Pro Thr Asn Thr Tyr Lys Thr Leu Glu Leu 
                165                 170                 175     


Glu Ile Ala Glu Phe Asp Val Lys Val Lys Glu Ala Glu Leu Glu Leu 
            180                 185                 190         


Val Lys Glu Glu Ala Lys Glu Ser Arg Asn Glu Gly Thr Ile Lys Gln 
        195                 200                 205             


Ala Lys Glu Lys Val Glu Ser Lys Lys Ala Glu Ala Thr Arg Leu Glu 
    210                 215                 220                 


Asn Ile Lys Thr Asp Arg Lys Lys Ala Glu Glu Glu Ala Lys Arg Lys 
225                 230                 235                 240 


Ala Asp Gly Lys Leu Lys Glu Ala Asn Val Ala Thr Ser Asp Gln Gly 
                245                 250                 255     


Lys Pro Lys Gly Arg Ala Lys Arg Gly Val Pro Gly Glu Leu Ala Thr 
            260                 265                 270         


Pro Asp Lys Lys Glu Asn Asp Ala Lys Ser Ser Asp Ser Ser Val Gly 
        275                 280                 285             


Glu Glu Thr Leu Pro Ser Ser Ser Leu Lys Ser Gly Lys Lys Val Ala 
    290                 295                 300                 


Glu Ala Glu Lys Lys Val Glu Glu Ala Glu Lys Lys Ala Lys Asp Gln 
305                 310                 315                 320 


Lys Glu Glu Asp Arg Arg Asn Tyr Pro Thr Asn Thr Tyr Lys Thr Leu 
                325                 330                 335     


Asp Leu Glu Ile Ala Glu Ser Asp Val Lys Val Lys Glu Ala Glu Leu 
            340                 345                 350         


Glu Leu Val Lys Glu Glu Ala Lys Glu Pro Arg Asp Glu Glu Lys Ile 
        355                 360                 365             


Lys Gln Ala Lys Ala Lys Val Glu Ser Lys Lys Ala Glu Ala Thr Arg 
    370                 375                 380                 


Leu Glu Asn Ile Lys Thr Asp Arg Lys Lys Ala Glu Glu Glu Ala Lys 
385                 390                 395                 400 


Arg Lys Ala Ala Glu Glu Asp Lys Val Lys Glu Lys Pro Ala Glu Gln 
                405                 410                 415     


Pro Gln Pro Ala Pro Ala Thr Gln Pro Glu Lys Pro Ala Pro Lys Pro 
            420                 425                 430         


Glu Lys Pro Ala Glu Gln Pro Lys Ala Glu Lys Thr Asp Asp Gln Gln 
        435                 440                 445             


Ala Glu Glu Asp Tyr Ala Arg Arg Ser Glu Glu Glu Tyr Asn Arg Leu 
    450                 455                 460                 


Thr Gln Gln Gln Pro Pro Lys Thr Glu Lys Pro Ala Gln Pro Ser Thr 
465                 470                 475                 480 


Pro Lys Thr Gly Trp Lys Gln Glu Asn Gly Met Trp Tyr Phe Tyr Asn 
                485                 490                 495     


Thr Asp Gly Ser Met Ala Thr Gly Trp Leu Gln Asn Asn Gly Ser Trp 
            500                 505                 510         


Tyr Tyr Leu Asn Ala Asn Gly Ala Met Ala Thr Gly Trp Leu Gln Asn 
        515                 520                 525             


Asn Gly Ser Trp Tyr Tyr Leu Asn Ala Asn Gly Ser Met Ala Thr Gly 
    530                 535                 540                 


Trp Leu Gln Asn Asn Gly Ser Trp Tyr Tyr Leu Asn Ala Asn Gly Ala 
545                 550                 555                 560 


Met Ala Thr Gly Trp Leu Gln Tyr Asn Gly Ser Trp Tyr Tyr Leu Asn 
                565                 570                 575     


Ser Asn Gly Ala Met Ala Thr Gly Trp Leu Gln Tyr Asn Gly Ser Trp 
            580                 585                 590         


Tyr Tyr Leu Asn Ala Asn Gly Asp Met Ala Thr Gly Trp Leu Gln Asn 
        595                 600                 605             


Asn Gly Ser Trp Tyr Tyr Leu Asn Ala Asn Gly Asp Met Ala Thr Gly 
    610                 615                 620                 


Trp Leu Gln Tyr Asn Gly Ser Trp Tyr Tyr Leu Asn Ala Asn Gly Asp 
625                 630                 635                 640 


Met Ala Thr Gly Trp Val Lys Asp Gly Xaa Thr Trp Tyr Tyr Leu Lys 
                645                 650                 655     


Ala Ser Gly Ala Met Lys Ala Ser Gln Trp Phe Lys Val Ser Asp Lys 
            660                 665                 670         


Trp Tyr Tyr Val Asn Gly Ser Gly Ala Leu Ala Val Asn Thr Thr Val 
        675                 680                 685             


Asp Gly Tyr Gly Val Asn Ala Asn Gly Glu Trp Val Asn 
    690                 695                 700     


<210>  5
<211>  315
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon-optimised DNA sequence used to express PspCN

<400>  5
gcaaccgaaa atgaaggtag cacccaggca gcaaccagca gcaatatggc aaaaaccgaa       60

catcgtaaag cagccaaaca ggttgtggat gagtatatcg aaaaaatgct gcgtgaaatt      120

cagctggatc gtcgtaaaca tacccagaat gttgcactga acattaaact gagcgccatc      180

aaaaccaaat atctgcgtga actgaatgtg ctggaagaga aaagcaaaga tgaactgccg      240

agcgaaatta aagcaaaact ggatgcagcc tttgaaaaat tcaaaaaaga taccctgaaa      300

ccgggtgaga aataa                                                       315


<210>  6
<211>  1231
<212>  PRT
<213>  Homo sapiens

<400>  6

Met Arg Leu Leu Ala Lys Ile Ile Cys Leu Met Leu Trp Ala Ile Cys 
1               5                   10                  15      


Val Ala Glu Asp Cys Asn Glu Leu Pro Pro Arg Arg Asn Thr Glu Ile 
            20                  25                  30          


Leu Thr Gly Ser Trp Ser Asp Gln Thr Tyr Pro Glu Gly Thr Gln Ala 
        35                  40                  45              


Ile Tyr Lys Cys Arg Pro Gly Tyr Arg Ser Leu Gly Asn Val Ile Met 
    50                  55                  60                  


Val Cys Arg Lys Gly Glu Trp Val Ala Leu Asn Pro Leu Arg Lys Cys 
65                  70                  75                  80  


Gln Lys Arg Pro Cys Gly His Pro Gly Asp Thr Pro Phe Gly Thr Phe 
                85                  90                  95      


Thr Leu Thr Gly Gly Asn Val Phe Glu Tyr Gly Val Lys Ala Val Tyr 
            100                 105                 110         


Thr Cys Asn Glu Gly Tyr Gln Leu Leu Gly Glu Ile Asn Tyr Arg Glu 
        115                 120                 125             


Cys Asp Thr Asp Gly Trp Thr Asn Asp Ile Pro Ile Cys Glu Val Val 
    130                 135                 140                 


Lys Cys Leu Pro Val Thr Ala Pro Glu Asn Gly Lys Ile Val Ser Ser 
145                 150                 155                 160 


Ala Met Glu Pro Asp Arg Glu Tyr His Phe Gly Gln Ala Val Arg Phe 
                165                 170                 175     


Val Cys Asn Ser Gly Tyr Lys Ile Glu Gly Asp Glu Glu Met His Cys 
            180                 185                 190         


Ser Asp Asp Gly Phe Trp Ser Lys Glu Lys Pro Lys Cys Val Glu Ile 
        195                 200                 205             


Ser Cys Lys Ser Pro Asp Val Ile Asn Gly Ser Pro Ile Ser Gln Lys 
    210                 215                 220                 


Ile Ile Tyr Lys Glu Asn Glu Arg Phe Gln Tyr Lys Cys Asn Met Gly 
225                 230                 235                 240 


Tyr Glu Tyr Ser Glu Arg Gly Asp Ala Val Cys Thr Glu Ser Gly Trp 
                245                 250                 255     


Arg Pro Leu Pro Ser Cys Glu Glu Lys Ser Cys Asp Asn Pro Tyr Ile 
            260                 265                 270         


Pro Asn Gly Asp Tyr Ser Pro Leu Arg Ile Lys His Arg Thr Gly Asp 
        275                 280                 285             


Glu Ile Thr Tyr Gln Cys Arg Asn Gly Phe Tyr Pro Ala Thr Arg Gly 
    290                 295                 300                 


Asn Thr Ala Lys Cys Thr Ser Thr Gly Trp Ile Pro Ala Pro Arg Cys 
305                 310                 315                 320 


Thr Leu Lys Pro Cys Asp Tyr Pro Asp Ile Lys His Gly Gly Leu Tyr 
                325                 330                 335     


His Glu Asn Met Arg Arg Pro Tyr Phe Pro Val Ala Val Gly Lys Tyr 
            340                 345                 350         


Tyr Ser Tyr Tyr Cys Asp Glu His Phe Glu Thr Pro Ser Gly Ser Tyr 
        355                 360                 365             


Trp Asp His Ile His Cys Thr Gln Asp Gly Trp Ser Pro Ala Val Pro 
    370                 375                 380                 


Cys Leu Arg Lys Cys Tyr Phe Pro Tyr Leu Glu Asn Gly Tyr Asn Gln 
385                 390                 395                 400 


Asn Tyr Gly Arg Lys Phe Val Gln Gly Lys Ser Ile Asp Val Ala Cys 
                405                 410                 415     


His Pro Gly Tyr Ala Leu Pro Lys Ala Gln Thr Thr Val Thr Cys Met 
            420                 425                 430         


Glu Asn Gly Trp Ser Pro Thr Pro Arg Cys Ile Arg Val Lys Thr Cys 
        435                 440                 445             


Ser Lys Ser Ser Ile Asp Ile Glu Asn Gly Phe Ile Ser Glu Ser Gln 
    450                 455                 460                 


Tyr Thr Tyr Ala Leu Lys Glu Lys Ala Lys Tyr Gln Cys Lys Leu Gly 
465                 470                 475                 480 


Tyr Val Thr Ala Asp Gly Glu Thr Ser Gly Ser Ile Thr Cys Gly Lys 
                485                 490                 495     


Asp Gly Trp Ser Ala Gln Pro Thr Cys Ile Lys Ser Cys Asp Ile Pro 
            500                 505                 510         


Val Phe Met Asn Ala Arg Thr Lys Asn Asp Phe Thr Trp Phe Lys Leu 
        515                 520                 525             


Asn Asp Thr Leu Asp Tyr Glu Cys His Asp Gly Tyr Glu Ser Asn Thr 
    530                 535                 540                 


Gly Ser Thr Thr Gly Ser Ile Val Cys Gly Tyr Asn Gly Trp Ser Asp 
545                 550                 555                 560 


Leu Pro Ile Cys Tyr Glu Arg Glu Cys Glu Leu Pro Lys Ile Asp Val 
                565                 570                 575     


His Leu Val Pro Asp Arg Lys Lys Asp Gln Tyr Lys Val Gly Glu Val 
            580                 585                 590         


Leu Lys Phe Ser Cys Lys Pro Gly Phe Thr Ile Val Gly Pro Asn Ser 
        595                 600                 605             


Val Gln Cys Tyr His Phe Gly Leu Ser Pro Asp Leu Pro Ile Cys Lys 
    610                 615                 620                 


Glu Gln Val Gln Ser Cys Gly Pro Pro Pro Glu Leu Leu Asn Gly Asn 
625                 630                 635                 640 


Val Lys Glu Lys Thr Lys Glu Glu Tyr Gly His Ser Glu Val Val Glu 
                645                 650                 655     


Tyr Tyr Cys Asn Pro Arg Phe Leu Met Lys Gly Pro Asn Lys Ile Gln 
            660                 665                 670         


Cys Val Asp Gly Glu Trp Thr Thr Leu Pro Val Cys Ile Val Glu Glu 
        675                 680                 685             


Ser Thr Cys Gly Asp Ile Pro Glu Leu Glu His Gly Trp Ala Gln Leu 
    690                 695                 700                 


Ser Ser Pro Pro Tyr Tyr Tyr Gly Asp Ser Val Glu Phe Asn Cys Ser 
705                 710                 715                 720 


Glu Ser Phe Thr Met Ile Gly His Arg Ser Ile Thr Cys Ile His Gly 
                725                 730                 735     


Val Trp Thr Gln Leu Pro Gln Cys Val Ala Ile Asp Lys Leu Lys Lys 
            740                 745                 750         


Cys Lys Ser Ser Asn Leu Ile Ile Leu Glu Glu His Leu Lys Asn Lys 
        755                 760                 765             


Lys Glu Phe Asp His Asn Ser Asn Ile Arg Tyr Arg Cys Arg Gly Lys 
    770                 775                 780                 


Glu Gly Trp Ile His Thr Val Cys Ile Asn Gly Arg Trp Asp Pro Glu 
785                 790                 795                 800 


Val Asn Cys Ser Met Ala Gln Ile Gln Leu Cys Pro Pro Pro Pro Gln 
                805                 810                 815     


Ile Pro Asn Ser His Asn Met Thr Thr Thr Leu Asn Tyr Arg Asp Gly 
            820                 825                 830         


Glu Lys Val Ser Val Leu Cys Gln Glu Asn Tyr Leu Ile Gln Glu Gly 
        835                 840                 845             


Glu Glu Ile Thr Cys Lys Asp Gly Arg Trp Gln Ser Ile Pro Leu Cys 
    850                 855                 860                 


Val Glu Lys Ile Pro Cys Ser Gln Pro Pro Gln Ile Glu His Gly Thr 
865                 870                 875                 880 


Ile Asn Ser Ser Arg Ser Ser Gln Glu Ser Tyr Ala His Gly Thr Lys 
                885                 890                 895     


Leu Ser Tyr Thr Cys Glu Gly Gly Phe Arg Ile Ser Glu Glu Asn Glu 
            900                 905                 910         


Thr Thr Cys Tyr Met Gly Lys Trp Ser Ser Pro Pro Gln Cys Glu Gly 
        915                 920                 925             


Leu Pro Cys Lys Ser Pro Pro Glu Ile Ser His Gly Val Val Ala His 
    930                 935                 940                 


Met Ser Asp Ser Tyr Gln Tyr Gly Glu Glu Val Thr Tyr Lys Cys Phe 
945                 950                 955                 960 


Glu Gly Phe Gly Ile Asp Gly Pro Ala Ile Ala Lys Cys Leu Gly Glu 
                965                 970                 975     


Lys Trp Ser His Pro Pro Ser Cys Ile Lys Thr Asp Cys Leu Ser Leu 
            980                 985                 990         


Pro Ser Phe Glu Asn Ala Ile Pro  Met Gly Glu Lys Lys  Asp Val Tyr 
        995                 1000                 1005             


Lys Ala  Gly Glu Gln Val Thr  Tyr Thr Cys Ala Thr  Tyr Tyr Lys 
    1010                 1015                 1020             


Met Asp  Gly Ala Ser Asn Val  Thr Cys Ile Asn Ser  Arg Trp Thr 
    1025                 1030                 1035             


Gly Arg  Pro Thr Cys Arg Asp  Thr Ser Cys Val Asn  Pro Pro Thr 
    1040                 1045                 1050             


Val Gln  Asn Ala Tyr Ile Val  Ser Arg Gln Met Ser  Lys Tyr Pro 
    1055                 1060                 1065             


Ser Gly  Glu Arg Val Arg Tyr  Gln Cys Arg Ser Pro  Tyr Glu Met 
    1070                 1075                 1080             


Phe Gly  Asp Glu Glu Val Met  Cys Leu Asn Gly Asn  Trp Thr Glu 
    1085                 1090                 1095             


Pro Pro  Gln Cys Lys Asp Ser  Thr Gly Lys Cys Gly  Pro Pro Pro 
    1100                 1105                 1110             


Pro Ile  Asp Asn Gly Asp Ile  Thr Ser Phe Pro Leu  Ser Val Tyr 
    1115                 1120                 1125             


Ala Pro  Ala Ser Ser Val Glu  Tyr Gln Cys Gln Asn  Leu Tyr Gln 
    1130                 1135                 1140             


Leu Glu  Gly Asn Lys Arg Ile  Thr Cys Arg Asn Gly  Gln Trp Ser 
    1145                 1150                 1155             


Glu Pro  Pro Lys Cys Leu His  Pro Cys Val Ile Ser  Arg Glu Ile 
    1160                 1165                 1170             


Met Glu  Asn Tyr Asn Ile Ala  Leu Arg Trp Thr Ala  Lys Gln Lys 
    1175                 1180                 1185             


Leu Tyr  Ser Arg Thr Gly Glu  Ser Val Glu Phe Val  Cys Lys Arg 
    1190                 1195                 1200             


Gly Tyr  Arg Leu Ser Ser Arg  Ser His Thr Leu Arg  Thr Thr Cys 
    1205                 1210                 1215             


Trp Asp  Gly Lys Leu Glu Tyr  Pro Thr Cys Ala Lys  Arg 
    1220                 1225                 1230     


<210>  7
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminally Cys modified PspCN

<400>  7

Cys Gly Ser Gly Ser Gly Ser Gly Ser Gly Gly Ala Thr Glu Asn Glu 
1               5                   10                  15      


Gly Ser Thr Gln Ala Ala Thr Ser Ser Asn Met Ala Lys Thr Glu His 
            20                  25                  30          


Arg Lys Ala Ala Lys Gln Val Val Asp Glu Tyr Ile Glu Lys Met Leu 
        35                  40                  45              


Arg Glu Ile Gln Leu Asp Arg Arg Lys His Thr Gln Asn Val Ala Leu 
    50                  55                  60                  


Asn Ile Lys Leu Ser Ala Ile Lys Thr Lys Tyr Leu Arg Glu Leu Asn 
65                  70                  75                  80  


Val Leu Glu Glu Lys Ser Lys Asp Glu Leu Pro Ser Glu Ile Lys Ala 
                85                  90                  95      


Lys Leu Asp Ala Ala Phe Glu Lys Phe Lys Lys Asp Thr Leu Lys Pro 
            100                 105                 110         


Gly Glu Lys 
        115 


<210>  8
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C-terminally Cys modified PspCN

<400>  8

Ala Thr Glu Asn Glu Gly Ser Thr Gln Ala Ala Thr Ser Ser Asn Met 
1               5                   10                  15      


Ala Lys Thr Glu His Arg Lys Ala Ala Lys Gln Val Val Asp Glu Tyr 
            20                  25                  30          


Ile Glu Lys Met Leu Arg Glu Ile Gln Leu Asp Arg Arg Lys His Thr 
        35                  40                  45              


Gln Asn Val Ala Leu Asn Ile Lys Leu Ser Ala Ile Lys Thr Lys Tyr 
    50                  55                  60                  


Leu Arg Glu Leu Asn Val Leu Glu Glu Lys Ser Lys Asp Glu Leu Pro 
65                  70                  75                  80  


Ser Glu Ile Lys Ala Lys Leu Asp Ala Ala Phe Glu Lys Phe Lys Lys 
                85                  90                  95      


Asp Thr Leu Lys Pro Gly Glu Lys Gly Ser Gly Ser Gly Ser Gly Ser 
            100                 105                 110         


Gly Gly Cys 
        115 


<210>  9
<211>  143
<212>  PRT
<213>  Streptococcus pneumoniae

<400>  9

Ala Thr Glu Asn Glu Gly Ala Thr Gln Val Pro Thr Ser Ser Asn Arg 
1               5                   10                  15      


Ala Asn Glu Ser Gln Ala Glu Gln Gly Glu Gln Pro Lys Lys Leu Asp 
            20                  25                  30          


Ser Glu Arg Asp Lys Ala Arg Lys Glu Val Glu Glu Tyr Val Lys Lys 
        35                  40                  45              


Ile Val Gly Glu Ser Tyr Ala Lys Ser Thr Lys Lys Arg His Thr Ile 
    50                  55                  60                  


Thr Val Ala Leu Val Asn Glu Leu Asn Asn Ile Lys Asn Glu Tyr Leu 
65                  70                  75                  80  


Asn Lys Ile Val Glu Ser Thr Ser Glu Ser Gln Leu Gln Ile Leu Met 
                85                  90                  95      


Met Glu Ser Arg Ser Lys Val Asp Glu Ala Val Ser Lys Phe Glu Lys 
            100                 105                 110         


Asp Ser Ser Ser Ser Ser Ser Ser Asp Ser Ser Thr Lys Pro Glu Ala 
        115                 120                 125             


Ser Asp Thr Ala Lys Pro Asn Lys Pro Thr Glu Pro Gly Glu Lys 
    130                 135                 140             


