               SEQUENCE LISTING

<110> DSM IP Assets B.V.

<120> Recovery of oil using PEP

<130> 29996-WO-PCT

<160> 2

<170> BiSSAP 1.2

<210> 1
<211> 526
<212> PRT
<213> proline-specific endoprotease


<400> 1
Met Arg Ala Phe Ser Ala Val Ala Ala Ala Ala Leu Ala Leu Ser Trp 
1               5                   10                  15      
Ala Ser Leu Ala Gln Ala Ala Arg Pro Arg Leu Val Pro Lys Pro Val 
            20                  25                  30          
Ser Arg Pro Ala Ser Ser Lys Ser Ala Ala Thr Thr Gly Glu Ala Tyr 
        35                  40                  45              
Phe Glu Gln Leu Leu Asp His His Asn Pro Glu Lys Gly Thr Phe Ser 
    50                  55                  60                  
Gln Arg Tyr Trp Trp Ser Thr Glu Tyr Trp Gly Gly Pro Gly Ser Pro 
65                  70                  75                  80  
Val Val Leu Phe Thr Pro Gly Glu Val Ser Ala Asp Gly Tyr Glu Gly 
                85                  90                  95      
Tyr Leu Thr Asn Glu Thr Leu Thr Gly Val Tyr Ala Gln Glu Ile Gln 
            100                 105                 110         
Gly Ala Val Ile Leu Ile Glu His Arg Tyr Trp Gly Asp Ser Ser Pro 
        115                 120                 125             
Tyr Glu Val Leu Asn Ala Glu Thr Leu Gln Tyr Leu Thr Leu Asp Gln 
    130                 135                 140                 
Ala Ile Leu Asp Met Thr Tyr Phe Ala Glu Thr Val Lys Leu Gln Phe 
145                 150                 155                 160 
Asp Asn Ser Thr Arg Ser Asn Ala Gln Asn Ala Pro Trp Val Met Val 
                165                 170                 175     
Gly Gly Ser Tyr Ser Gly Ala Leu Thr Ala Trp Thr Glu Ser Val Ala 
            180                 185                 190         
Pro Gly Thr Phe Trp Ala Tyr His Ala Thr Ser Ala Pro Val Glu Ala 
        195                 200                 205             
Ile Tyr Asp Tyr Trp Gln Tyr Phe Tyr Pro Ile Gln Gln Gly Met Ala 
    210                 215                 220                 
Gln Asn Cys Ser Lys Asp Val Ser Leu Val Ala Glu Tyr Val Asp Lys 
225                 230                 235                 240 
Ile Gly Lys Asn Gly Thr Ala Lys Glu Gln Gln Ala Leu Lys Glu Leu 
                245                 250                 255     
Phe Gly Leu Gly Ala Val Glu His Phe Asp Asp Phe Ala Ala Val Leu 
            260                 265                 270         
Pro Asn Gly Pro Tyr Leu Trp Gln Asp Asn Asp Phe Ala Thr Gly Tyr 
        275                 280                 285             
Ser Ser Phe Phe Gln Phe Cys Asp Ala Val Glu Gly Val Glu Ala Gly 
    290                 295                 300                 
Ala Ala Val Thr Pro Gly Pro Glu Gly Val Gly Leu Glu Lys Ala Leu 
305                 310                 315                 320 
Ala Asn Tyr Ala Asn Trp Phe Asn Ser Thr Ile Leu Pro Asp Tyr Cys 
                325                 330                 335     
Ala Ser Tyr Gly Tyr Trp Thr Asp Glu Trp Ser Val Ala Cys Phe Asp 
            340                 345                 350         
Ser Tyr Asn Ala Ser Ser Pro Ile Tyr Thr Asp Thr Ser Val Gly Asn 
        355                 360                 365             
Ala Val Asp Arg Gln Trp Glu Trp Phe Leu Cys Asn Glu Pro Phe Phe 
    370                 375                 380                 
Tyr Trp Gln Asp Gly Ala Pro Glu Gly Thr Ser Thr Ile Val Pro Arg 
385                 390                 395                 400 
Leu Val Ser Ala Ser Tyr Trp Gln Arg Gln Cys Pro Leu Tyr Phe Pro 
                405                 410                 415     
Glu Thr Asn Gly Tyr Thr Tyr Gly Ser Ala Lys Gly Lys Asn Ala Ala 
            420                 425                 430         
Thr Val Asn Ser Trp Thr Gly Gly Trp Asp Met Thr Arg Asn Thr Thr 
        435                 440                 445             
Arg Leu Ile Trp Thr Asn Gly Gln Tyr Asp Pro Trp Arg Asp Ser Gly 
    450                 455                 460                 
Val Ser Ser Thr Phe Arg Pro Gly Gly Pro Leu Ala Ser Thr Ala Asn 
465                 470                 475                 480 
Glu Pro Val Gln Ile Ile Pro Gly Gly Phe His Cys Ser Asp Leu Tyr 
                485                 490                 495     
Met Ala Asp Tyr Tyr Ala Asn Glu Gly Val Lys Lys Val Val Asp Asn 
            500                 505                 510         
Glu Val Lys Gln Ile Lys Glu Trp Val Glu Glu Tyr Tyr Ala 
        515                 520                 525     

<210> 2
<211> 1578
<212> DNA
<213> Aspergillus niger

<220> 
<221> source
<222> 1..1578


<400> 2
atgcgtgcct tctccgctgt cgctgctgcg gccctggcgc tctcttgggc gtctctggct      60

caggctgctc gcccccgtct tgtgcccaag cctgtctctc ggccagcctc gagtaaatcg     120

gctgcgacca cgggcgaggc ttactttgag cagctgctgg accatcataa tccggagaag     180

ggcacctttt cccagaggta ctggtggagt actgaatact ggggtggtcc tgggtcaccg     240

gtcgtcctct ttactcctgg agaggtctct gccgatggct atgaggggta tctcaccaat     300

gagactctca ctggtgtcta tgcgcaggag atccagggtg ccgtcattct cattgagcac     360

cgctactggg gtgattcttc tccttatgag gtgctcaatg ccgaaactct tcagtacctc     420

acactggacc aagccattct ggacatgacc tacttcgccg agacggtgaa gctgcaattc     480

gataacagca cccgcagcaa tgcgcagaat gctccctggg tcatggtcgg tggatcatac     540

agtggtgcct tgacggcttg gaccgaatct gtcgcgcctg gaacgttctg ggcttaccat     600

gccactagtg ctcctgtgga ggctatctac gactattggc aatactttta ccccatccag     660

caaggtatgg cacagaactg cagcaaggac gtgtctctgg tagccgagta tgtcgacaag     720

attggaaaga acggaactgc caaggagcag caggcactca aggaattgtt tggtctggga     780

gctgttgagc attttgatga ctttgccgct gtcctcccca acggaccgta cctctggcaa     840

gacaacgact ttgccacggg atactcttcc ttcttccagt tctgtgacgc cgtcgagggt     900

gtcgaagccg gcgcggcagt aacccccggc cccgagggtg tcggcctcga aaaggccctg     960

gccaactacg caaactggtt caattcaacc attctccctg attactgcgc aagctacggc    1020

tactggaccg acgaatggag cgtcgcctgc ttcgacagct acaacgcctc gagccccatc    1080

tacaccgata cctccgtagg caatgccgtc gaccgccaat gggaatggtt cctctgcaac    1140

gagcctttct tctactggca ggacggtgct cccgagggta cctccaccat tgtgccccga    1200

ctcgtcagcg cctcctactg gcaacggcaa tgtccgctct acttccccga aacgaacggc    1260

tacacgtacg gcagcgcgaa gggtaagaac gccgccacgg tgaacagctg gaccggtgga    1320

tgggatatga cccgcaacac gacgcggttg atctggacga acgggcaata tgacccctgg    1380

cgggactccg gtgtgtcgag cactttccgg cccggtggac cgctggcgag cacggcgaat    1440

gaacccgtgc agattatccc gggcggattc cattgctcgg atttgtatat ggcggattat    1500

tatgcgaatg agggggttaa aaaggtggtg gataatgagg tgaagcagat caaggagtgg    1560

gtggaggagt attatgcc                                                  1578


