                         SEQUENCE LISTING

<110>  Technical University of Denmark
 
<120>  Multiplex editing system

<130>  P3268PC00

<160>  112   

<170>  PatentIn version 3.5

<210>  1
<211>  117
<212>  DNA
<213>  Unknown

<220>
<223>  Guide RNA scaffold


<220>
<221>  misc_binding
<222>  (1)..(117)
<223>  Guide RNA scaffold

<400>  1
gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt tttctagacc cagctttctt gtacaaagtt ggcatta         117


<210>  2
<211>  4140
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Cas9 sequence, codon-optimised for CHO cell


<220>
<221>  CDS
<222>  (1)..(4140)
<223>  Cas9 sequence, codon-optimised for CHO cell

<400>  2
atggacaaga aatactccat cggccttgat atcgggacca acagcgtggg ttgggccgtg       60

atcactgacg aatacaaagt gccatcgaaa aagtttaagg tgctgggcaa tactgacaga      120

catagcatca agaaaaacct gattggagcc ctcctgttcg actccgggga aaccgccgaa      180

gccactcgcc tgaaacggac cgcacgccgg aggtacactc gccggaagaa tagaatctgc      240

taccttcaag agatctttag caacgagatg gccaaagtgg acgattcgtt cttccaccgg      300

ctggaggagt cattcctggt cgaagaggac aaaaagcatg agagacatcc gatcttcggt      360

aacattgtgg atgaggtcgc ctaccacgag aagtacccca ctatctacca tttgcgcaag      420

aagttggtcg actcgactga taaggccgac ctcagactca tctacctcgc tttggcacac      480

atgatcaagt ttaggggtca cttcctgatt gaaggggatc tgaacccaga caactcggat      540

gtggataaac tgtttatcca gctggtccag acttacaatc agctctttga ggagaacccg      600

atcaatgcct ccggcgtgga tgctaaggca atcctgtccg cacgcttgtc aaagagccgg      660

cggctcgaaa atctgatcgc acagcttccg ggagaaaaga aaaacggact gtttggaaac      720

cttatcgccc tctcgctggg actcactcct aacttcaaat ccaattttga tttggccgag      780

gatgccaagc tgcagctgtc gaaggacacc tacgacgatg acctcgacaa tcttctggcc      840

cagatcgggg accagtacgc cgatctgttc ctcgcggcca aaaacctgtc agacgcaatc      900

ttgctgagcg atatcttgag ggtcaatacc gaaatcacta aggctccatt gtcagcatcg      960

atgatcaaga ggtacgatga acaccatcag gacctgaccc tgctcaaggc actggtgcgg     1020

cagcaactgc cggaaaagta caaggagatc ttcttcgatc aaagcaagaa tgggtacgca     1080

gggtacatcg atggtggagc atcacaagaa gagttctaca aattcatcaa gcccatcctt     1140

gaaaagatgg acgggacgga agaactgctg gtgaagctga atcgggaaga tctgctgcgg     1200

aagcagcgca ccttcgacaa tggttccatc ccacaccaaa tccatctcgg cgaactgcac     1260

gcgatccttc gccggcagga agatttctac ccgttcttga aggataacag agaaaagatc     1320

gagaaaatcc tgacctttag aatcccgtac tacgtgggcc cgttggctcg cggaaactca     1380

agattcgcct ggatgactag aaaatccgaa gaaacgatta ccccgtggaa ctttgaagag     1440

gtcgtcgaca agggagcctc ggcacagtcg ttcatcgaac ggatgaccaa tttcgacaag     1500

aacctcccca acgaaaaggt gctcccgaaa cactcgctgc tgtatgagta cttcacggtc     1560

tacaacgagc tgaccaaggt gaagtacgtg accgaaggaa tgcggaagcc cgcttttctg     1620

tccggtgaac agaaaaaggc catcgtggac ctcctcttca agactaacag aaaggtgacc     1680

gtgaaacagc ttaaagagga ctacttcaag aagatcgagt gttttgactc cgtggaaatc     1740

tcgggagtgg aggataggtt taacgcttcg ctggggacct accatgacct tctcaagatc     1800

atcaaggata aggatttcct ggacaacgaa gaaaacgagg acatccttga ggacatcgtg     1860

ttgaccttga ccctgtttga ggatcgggaa atgatcgagg agagactgaa aacttacgcg     1920

catcttttcg acgacaaagt gatgaagcag ctgaaaagaa gaagatacac tggctgggga     1980

cggctgtcga ggaaactgat taacggaatt agggataaac aaagcggaaa gactatcctc     2040

gattttctca agtcggacgg cttcgccaat cggaacttta tgcagctcat ccacgacgat     2100

agcctgacct tcaaggaaga tatccaaaaa gcccaagtgt ccggccaggg agatagcttg     2160

cacgagcaca ttgctaacct ggccggttca ccagccatta agaagggaat cctccaaacc     2220

gtgaaggtgg tcgacgaact cgtcaaggtg atgggccgcc ataagccaga gaacatcgtg     2280

atcgagatgg ctcgcgaaaa tcagactact cagaagggtc aaaagaactc ccgcgagcgc     2340

atgaagcgca tcgaagaagg aattaaggag ctgggctcac aaatcctcaa agaacatcct     2400

gtcgagaaca cccaacttca gaacgagaaa ctgtacctct actatctcca aaatggccgg     2460

gacatgtacg tcgatcagga attggatatc aaccgcctct cagactacga cgtggatcat     2520

atcgtgccgc agtcgtttct caaagatgat agcatcgaca acaaggtcct gacgaggtcg     2580

gataagaacc gcgggaaatc cgacaatgtg ccttcggaag aggtggtgaa aaagatgaag     2640

aactactgga ggcaattgct taatgccaaa ctgatcaccc agcgcaaatt cgacaacctt     2700

actaaggccg agcgcggagg actgtccgaa ctcgataagg cggggttcat caaaagacaa     2760

ttggtcgaaa ctcggcaaat taccaagcat gtcgctcaga tcctcgactc gcgcatgaac     2820

actaagtatg atgagaacga caagctcatt cgcgaagtga aagtgattac cctcaaatca     2880

aagctggtgt cggacttcag aaaagacttt caattctaca aggtgcggga gattaacaac     2940

taccaccacg cgcacgacgc ctacctcaat gcagtggtgg gaaccgccct catcaagaag     3000

tatccaaagc tcgaaagcga gttcgtgtac ggggactata aagtgtacga tgtgcggaaa     3060

atgatcgcaa agagcgagca agagatcggc aaagcaacgg ccaaatactt cttctacagc     3120

aacattatga actttttcaa gaccgagatt accctggcga acggagaaat ccggaagcgg     3180

cctttgatcg aaacgaatgg ggaaactgga gaaatcgtgt gggacaaggg cagagacttt     3240

gcgaccgtga gaaaggtgct gtccatgcca caagtcaaca tcgtgaagaa aactgaagtg     3300

cagactggag gattttccaa ggagtcaatc cttccgaagc gcaacagcga caagctgatc     3360

gccaggaaga aggattggga ccccaagaag tacggtggtt ttgattcacc tactgtcgct     3420

tacagcgtgc tggtggtggc caaggtggaa aaggggaagt caaagaaatt gaagtcggtg     3480

aaggaattgc tggggattac tatcatggag aggagcagct tcgaaaagaa tcccatcgac     3540

ttcttggagg ccaagggata caaagaggtg aagaaagacc tgatcattaa gctgccgaag     3600

tactccttgt tcgaactgga aaacggcaga aagcggatgc tggcctccgc cggagagctg     3660

cagaagggta acgagctggc tctgcccagc aaatacgtga atttcctcta cctggcctcc     3720

cattacgaga agctcaaggg aagcccggag gataatgagc aaaaacaact gttcgtcgag     3780

cagcacaagc actacctcga cgagatcatc gaacaaatct ccgagttctc gaagcgggtg     3840

attctggccg acgcaaacct tgataaagtc ctcagcgcct acaacaagca ccgcgacaaa     3900

ccgatcagag aacaagctga gaacatcatc cacctgttca cgctgactaa cttgggagcg     3960

cctgccgcct tcaagtactt cgacaccact atcgatcgga aacggtacac ttccactaag     4020

gaagtgctgg acgcaaccct gatccatcag tccatcaccg gactttatga gactcggatc     4080

gatctcagcc agcttggcgg tgattcaaga gctgacccga agaagaagcg caaggtctag     4140


<210>  3
<211>  34
<212>  PRT
<213>  Artificial sequence

<220>
<223>  DNA binding domain of TALENS


<220>
<221>  DNAbinding_domain
<222>  (1)..(34)
<223>  DNA binding domain of TALENS. "Xaa" stands for any amino acid.

<400>  3

Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Xaa Xaa Gly Gly Lys 
1               5                   10                  15      


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala 
            20                  25                  30          


His Gly 
        


<210>  4
<211>  264
<212>  DNA
<213>  Unknown

<220>
<223>  Artificial sequence


<220>
<221>  U6_promoter
<222>  (1)..(264)

<220>
<221>  U6_promoter
<222>  (1)..(264)
<223>  U6 promoter sequence

<220>
<221>  U6_promoter
<222>  (1)..(264)
<223>  U6 promoter sequence

<400>  4
aaggtcgggc aggaagaggg cctatttccc atgattcctt catatttgca tatacgatac       60

aaggctgtta gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa      120

aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt      180

aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat      240

atcttgtgga aaggacgaaa cacc                                             264


<210>  5
<211>  34
<212>  DNA
<213>  Unknown

<220>
<223>  Artificial sequence


<220>
<221>  misc_feature
<222>  (1)..(34)

<220>
<221>  misc_feature
<222>  (1)..(34)
<223>  Scaffold sequence

<400>  5
gttttagagc tagaaatagc aagttaaaat aagg                                   34


<210>  6
<211>  49
<212>  DNA
<213>  Unknown

<220>
<223>  Artificial sequence


<220>
<221>  Terminator
<222>  (1)..(49)

<220>
<221>  Terminator
<222>  (1)..(49)
<223>  Terminator sequence

<400>  6
ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttt                   49


<210>  7
<211>  21
<212>  DNA
<213>  Unknown

<220>
<223>  Artificial sequence


<220>
<221>  misc_binding
<222>  (1)..(21)
<223>  gRNA_target

<400>  7
gaaaagtgtc ctgaacaagg t                                                 21


<210>  8
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(20)
<223>  Primer_1864

<400>  8
aggccctatt gatcagggga                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(20)
<223>  Primer 1865

<400>  9
tggaagccca aatgaagcac                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(20)
<223>  Primer 1868

<400>  10
ggtcgagctc cccattgtag                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(20)
<223>  Primer 1869

<400>  11
gctctgctgc cctaactgaa                                                   20


<210>  12
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(19)
<223>  primer 1878

<400>  12
gcccccatga ctagggata                                                    19


<210>  13
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(21)
<223>  Primer 1879

<400>  13
cccatacaga accacttgtt g                                                 21


<210>  14
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(20)
<223>  Primer 1884

<400>  14
cccagagtcc atgtcagacg                                                   20


<210>  15
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(22)
<223>  Primer 1885

<400>  15
gcaacaagaa ccacaagttc cc                                                22


<210>  16
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(20)
<223>  COSMC_primer_forward

<400>  16
ggatccatcg cagcctttct                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(20)
<223>  COSMC primer reverse

<400>  17
aaccacccga accaggtagt                                                   20


<210>  18
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  misc_feature
<222>  (1)..(23)
<223>  sgRNA_consensus; N = any nucleotide

<220>
<221>  misc_feature
<222>  (2)..(21)
<223>  n is a, c, g, or t

<400>  18
gnnnnnnnnn nnnnnnnnnn ngg                                               23


<210>  19
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  misc_binding
<222>  (1)..(23)
<223>  COSMC_sgRNA1_C

<400>  19
gaaaagtgtc ctgaacaagg tgg                                               23


<210>  20
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  misc_binding
<222>  (1)..(23)
<223>  COSMC_sgRNA2_C


<400>  20
gaatatgtga gtgtggatgg agg                                               23


<210>  21
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  misc_binding
<222>  (1)..(23)
<223>  COSMC_sgRNA3_C

<400>  21
gaaatatgct ggagtatttg cgg                                               23


<210>  22
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  misc_binding
<222>  (1)..(23)
<223>  COSMC_sgRNA4_C

<400>  22
gcagtctgcc tgaaatatgc tgg                                               23


<210>  23
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  misc_binding
<222>  (1)..(23)
<223>  FUT8_sgRNA1_F

<400>  23
gaaaggatca tgaaatctta agg                                               23


<210>  24
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  misc_binding
<222>  (1)..(23)
<223>  FUT8_sgRNA2_F

<400>  24
gatccgtcca caaccttggc tgg                                               23


<210>  25
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  misc_binding
<222>  (1)..(23)
<223>  FUT8_sgRNA3_F

<400>  25
gtcagacgca ctgacaaagt ggg                                               23


<210>  26
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  misc_binding
<222>  (1)..(23)
<223>  FUT8_sgRNA4_F

<400>  26
ggataaaaaa agagtgtatc tgg                                               23


<210>  27
<211>  54
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(54)
<223>  Sense oligo for sgRNA1_C; sgRNA1_C_fw

<400>  27
ggaaaggacg aaacaccgaa aagtgtcctg aacaagggtt ttagagctag aaat             54


<210>  28
<211>  53
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(53)
<223>  Antisense Oligo for sgRNA1_C; sgRNA1_C_rv

<400>  28
ctaaaaccct tgttcaggac acttttcggt gtttcgtcct ttccacaaga tat              53


<210>  29
<211>  54
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(54)
<223>  Sense oligo for sgRNA2_C; sgRNA2_C_fw

<400>  29
ggaaaggacg aaacaccgaa tatgtgagtg tggatgggtt ttagagctag aaat             54


<210>  30
<211>  53
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(53)
<223>  sgRNA2_C_rv

<400>  30
ctaaaaccca tccacactca catattcggt gtttcgtcct ttccacaaga tat              53


<210>  31
<211>  54
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(54)
<223>  Sense oligo for sgRNA3_C; sgRNA3_C_fw

<400>  31
ggaaaggacg aaacaccgaa atatgctgga gtatttggtt ttagagctag aaat             54


<210>  32
<211>  53
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(53)
<223>  Antisense Oligo for sgRNA3_C; sgRNA3_C_rv

<400>  32
ctaaaaccaa atactccagc atatttcggt gtttcgtcct ttccacaaga tat              53


<210>  33
<211>  54
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(54)
<223>  Sense oligo for sgRNA4_C; sgRNA4_C_fw

<400>  33
ggaaaggacg aaacaccgca gtctgcctga aatatgcgtt ttagagctag aaat             54


<210>  34
<211>  53
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(53)
<223>  Antisense Oligo for sgRNA4_C; sgRNA4_C_rv

<400>  34
ctaaaacgca tatttcaggc agactgcggt gtttcgtcct ttccacaaga tat              53


<210>  35
<211>  54
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(54)
<223>  Sense oligo for sgRNA1_F; sgRNA1_F_fw

<400>  35
ggaaaggacg aaacaccgaa aggatcatga aatcttagtt ttagagctag aaat             54


<210>  36
<211>  53
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(53)
<223>  Antisense Oligo for sgRNA1_F; sgRNA1_F_rv

<400>  36
ctaaaactaa gatttcatga tcctttcggt gtttcgtcct ttccacaaga tat              53


<210>  37
<211>  54
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(54)
<223>  Sense oligo for sgRNA2_F; sgRNA2_F_fw

<400>  37
ggaaaggacg aaacaccgat ccgtccacaa ccttggcgtt ttagagctag aaat             54


<210>  38
<211>  53
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(53)
<223>  Antisense Oligo for sgRNA2_F; sgRNA2_F_rv

<400>  38
ctaaaacgcc aaggttgtgg acggatcggt gtttcgtcct ttccacaaga tat              53


<210>  39
<211>  54
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(54)
<223>  Sense oligo for sgRNA3_F; sgRNA3_F_fw

<400>  39
ggaaaggacg aaacaccgtc agacgcactg acaaagtgtt ttagagctag aaat             54


<210>  40
<211>  53
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(53)
<223>  Antisense Oligo for sgRNA3_F; sgRNA3_F_rv

<400>  40
ctaaaacact ttgtcagtgc gtctgacggt gtttcgtcct ttccacaaga tat              53


<210>  41
<211>  54
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(54)
<223>  Sense oligo for sgRNA4_F; sgRNA4_F_fw

<400>  41
ggaaaggacg aaacaccgga taaaaaaaga gtgtatcgtt ttagagctag aaat             54


<210>  42
<211>  53
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(53)
<223>  Antisense Oligo for sgRNA4_F; sgRNA4_F_rv

<400>  42
ctaaaacgat acactctttt tttatccggt gtttcgtcct ttccacaaga tat              53


<210>  43
<211>  24
<212>  RNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(24)
<223>  USER PCR primer for sgRNA backbone amplicon; sgRNA Backbone_fw

<400>  43
agcagaaaua gcaagaaaaa aggc                                              24


<210>  44
<211>  24
<212>  RNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(24)
<223>  USER PCR primer for sgRNA backbone amplicon; sgRNA Backbone_rv

<400>  44
acaagaauaa aagccaagaa acga                                              24


<210>  45
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(20)
<223>  COSMC amplicon for TOPO cloning and T7 assay; COSMC-fw

<400>  45
ggatccatcg cagcctttct                                                   20


<210>  46
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(20)
<223>  COSMC amplicon for TOPO cloning and T7 assay; COSMC-rv

<400>  46
actacctggt tcgggtggtt                                                   20


<210>  47
<211>  16
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(16)
<223>  Sequencing primer for pCR4-TOPO vector; M13 forward (-20)

<400>  47
gtaaaacgac ggccag                                                       16


<210>  48
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(23)
<223>  COSMC amplicon for MiSeq analysis; gRNA1_C_fw_D

<400>  48
cgcattttcc gcaaatactc cag                                               23


<210>  49
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(24)
<223>  COSMC amplicon for MiSeq analysis; gRNA1_C_rv_D

<400>  49
tgaatatgtg agtgtggatg gagg                                              24


<210>  50
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(21)
<223>  COSMC amplicon for MiSeq analysis; gRNA2_C_fw_D

<400>  50
tcccaccttg ttcaggacac t                                                 21


<210>  51
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(22)
<223>  COSMC amplicon for MiSeq analysis; gRNA2_C_rv_D

<400>  51
ggatccatcg cagcctttct at                                                22


<210>  52
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(24)
<223>  COSMC amplicon for MiSeq analysis; gRNA3_C_fw_D

<400>  52
atgaaaagcc caacagattt ggta                                              24


<210>  53
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(22)
<223>  COSMC amplicon for MiSeq analysis; gRNA3_C_rv_D

<400>  53
agactcaaca gccttctcag tg                                                22


<210>  54
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(23)
<223>  gRNA4_C_fw_D

<400>  54
tgaaaagccc aacagatttg gta                                               23


<210>  55
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(21)
<223>  COSMC amplicon for MiSeq analysis; gRNA4_C_rv_D

<400>  55
agtgttccgg aaaagtgtcc t                                                 21


<210>  56
<211>  25
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(25)
<223>  FUT8 amplicon for MiSeq analysis; gRNA1_F_fw_D

<400>  56
agtgacctta gcacaaatat tgaaa                                             25


<210>  57
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(21)
<223>  FUT8 amplicon for MiSeq analysis; gRNA1_F_rv_D

<400>  57
tgtctttgga gttcgtttcc t                                                 21


<210>  58
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(20)
<223>  FUT8 amplicon for MiSeq analysis; gRNA2_F_fw_D

<400>  58
agagtccatg gtgatcctgc                                                   20


<210>  59
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(21)
<223>  FUT8 amplicon for MiSeq analysis; gRNA2_F_rv_D

<400>  59
tactgtttaa ggggaggggg a                                                 21


<210>  60
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(20)
<223>  FUT8 amplicon for MiSeq analysis; gRNA3_F_fw_D

<400>  60
tgcccccatg actagggata                                                   20


<210>  61
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(21)
<223>  FUT8 amplicon for MiSeq analysis; gRNA3_F_rv_D

<400>  61
tctgcgttcg agaagctgaa a                                                 21


<210>  62
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(20)
<223>  FUT8 amplicon for MiSeq analysis; gRNA4_F_fw_D

<400>  62
acagaagcag ccttccatcc                                                   20


<210>  63
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(22)
<223>  FUT8 amplicon for MiSeq analysis; gRNA4_F_rv_D

<400>  63
cccatacaga accacttgtt gg                                                22


<210>  64
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  misc_binding
<222>  (1)..(23)
<223>  C1GALT1/COSMC (sgRNA2_C); CRISPy identification number 1964768

<400>  64
gaatatgtga gtgtggatgg agg                                               23


<210>  65
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  misc_binding
<222>  (1)..(23)
<223>  Mgat1_sgRNA1; CRISPy identification number 905598

<400>  65
gctcacaccc ttacggccaa agg                                               23


<210>  66
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  misc_binding
<222>  (1)..(23)
<223>  Mgat1_sgRNA5; CRISPy identification number 904917

<400>  66
gtggagttgg agcggcagcg ggg                                               23


<210>  67
<211>  54
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(54)
<223>  Mgat1_sgRNA1_fwd

<400>  67
ggaaaggacg aaacaccgct cacaccctta cggccaagtt ttagagctag aaat             54


<210>  68
<211>  53
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(53)
<223>  Mgat1_sgRNA1_rev

<400>  68
ctaaaacttg gccgtaaggg tgtgagcggt gtttcgtcct ttccacaaga tat              53


<210>  69
<211>  54
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(54)
<223>  Mgat1_sgRNA5_fwd

<400>  69
ggaaaggacg aaacaccgtg gagttggagc ggcagcggtt ttagagctag aaat             54


<210>  70
<211>  53
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(53)
<223>  Mgat1_sgRNA5_rev

<400>  70
ctaaaaccgc tgccgctcca actccacggt gtttcgtcct ttccacaaga tat              53


<210>  71
<211>  9597
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  misc_feature
<222>  (1)..(9597)
<223>  COSMC-mCherry-HDR-TI donor

<400>  71
agtcggtgtg taatccatgg aggagtttct ataatgttgc agtttctacc taatggtgac       60

caaatgccag tgaaaggatt gtaagagtac ttgtcacata tactactcac ctcatttcaa      120

gaatgtggac ctgcttttaa acattaagag caaatcgtaa ttatataaga aataagcaaa      180

tgaaactatt agactgtttg aaaagtcttt ttctttacag gaaaaatgct ttcagaaagc      240

agttcatttt tgaaaggtgt gatgcttgga agcatcttct atgccttgat cactacgcta      300

ggccacatta ggattgggca cagaaacagg acacaccacc atgagcatca ccacctgcaa      360

gctcctaaca aagaagatat ctcgaaaatc tcagcggctg agcgcatgga gctcagtaag      420

agcttccggg tatactgtat agttcttgta aaacccaaag atgtgagtct ttgggctgca      480

gtgaaggaga cttggaccaa acactgtgac aaagcagagt tcttcagttc tgaaaatgtt      540

aaagtgtttg agtcaattaa cgtggacact gatgacatgt ggttgatgat gaggaaagct      600

tataaatatg cctttgataa atacaaagag cagtacaact ggttcttcct tgcacgcccc      660

agtacttttg ctgtgattga aaatctaaaa tattttttgt taaaaaagga tccatcgcag      720

cctttctatc taggacacac tgtaaaatct ggagacctta agcagcgtgt gaggctccgg      780

tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt      840

cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt      900

gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc      960

cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg     1020

ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac     1080

ctggctccag tacgtgattc ttgatcccga gctggagcca ggggcgggcc ttgcgcttta     1140

ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg     1200

cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa     1260

aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc     1320

caggatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg     1380

tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg     1440

gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc     1500

ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg     1560

cttcccggcc ctgctccagg gggctcaaaa tggaggacgc ggcgctcggg agagcgggcg     1620

ggtgagtcac ccacacaaag gaaaggggcc tttccgtcct cagccgtcgc ttcatgtgac     1680

tccacggagt accgggcgcc gtccaggcac ctcgattagt tctggagctt ttggagtacg     1740

tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg     1800

gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt     1860

gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca     1920

tttcaggtgt cgtgaagacg tcatcgccac catggtgagc aagggcgagg aggataacat     1980

ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca     2040

cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa     2100

gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt     2160

catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct     2220

gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt     2280

gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg     2340

cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc     2400

ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct     2460

gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa     2520

gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa     2580

cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg     2640

catggacgag ctgtacaagt agacacagtc tctgtgcctt ctagttgcca gccatctgtt     2700

gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc     2760

taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt     2820

ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat     2880

gcggtgggct ctatggaggt ctgagtctgt ggaatgtgtg tcagttaggg tgtggaaagt     2940

ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca     3000

ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt     3060

agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt     3120

ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg     3180

cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt     3240

gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag     3300

gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg     3360

agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt     3420

tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc     3480

tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt     3540

gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag     3600

tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg     3660

ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag     3720

cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg     3780

atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc     3840

gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca     3900

tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc     3960

gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg     4020

ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct     4080

atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc     4140

gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg     4200

cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct     4260

ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa     4320

tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc     4380

caaactcatc aatgtatctt atcatgtctg aggtctgagt gattgtctta agcatagagt     4440

caatgaaaag actcaacagc cttctcagtg ttccggaaaa gtgtcctgaa caaggtggga     4500

tgatttggaa gatatctgaa gataagcagc tagcagtctg cctgaaatat gctggagtat     4560

ttgcggaaaa tgcggaagac gctgatagaa aagatgtatt taataccaaa tctgttgggc     4620

ttttcattaa agaggccatg tctaaccacc cgaaccaggt agtagaagga tgctgttcca     4680

atatggctgt cacttttaat ggactaactc ctaatcagat gcatgtgatg atgtatgggg     4740

tgtaccggct tagggccttt ggacatgttt tcaacgatgc gttggttttc ttacctccaa     4800

acggttctga taatgactga caaaaagcaa gagcatgcat ttggtaacca cattaagaca     4860

tgttatgctt tctaatcgat aatgcatcta acacagtagt gtgtttcttt tccttatctg     4920

gtcacattga agtctacttg tacattttca aatggaatgg tatttttttc ccttaaatca     4980

tttgtgagaa attttaatgt gttagaaata aatgttttaa gaatagcaat tttgcaaata     5040

atgtatttat aaatattata tttatgtgat aaagaccaaa ttatagacat taaaatctgt     5100

gatgtatctt tgcctattga ttttaaatgt ttaatgtatc tttttagatt tcaaatatat     5160

gcaaatgagg acgtcgctgt tgacattgat tattgactag ttattaatag taatcaatta     5220

cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg     5280

gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc     5340

ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa     5400

ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca     5460

atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta     5520

cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt     5580

acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg     5640

acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca     5700

actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca     5760

gagctctctg gctaactaga gaacccactg cttactggct tatcgaaata gtgcgatcgc     5820

caccatggcc cagtccaagc acggcctgac caaggagatg accatgaagt accgcatgga     5880

gggctgcgtg gacggccaca agttcgtgat caccggcgag ggcatcggct accccttcaa     5940

gggcaagcag gccatcaacc tgtgcgtggt ggagggcggc cccttgccct tcgccgagga     6000

catcttgtcc gccgccttca tgtacggcaa ccgcgtgttc accgagtacc cccaggacat     6060

cgtcgactac ttcaagaact cctgccccgc cggctacacc tgggaccgct ccttcctgtt     6120

cgaggacggc gccgtgtgca tctgcaacgc cgacatcacc gtgagcgtgg aggagaactg     6180

catgtaccac gagtccaagt tctacggcgt gaacttcccc gccgacggcc ccgtgatgaa     6240

gaagatgacc gacaactggg agccctcctg cgagaagatc atccccgtgc ccaagcaggg     6300

catcttgaag ggcgacgtga gcatgtacct gctgctgaag gacggtggcc gcttgcgctg     6360

ccagttcgac accgtgtaca aggccaagtc cgtgccccgc aagatgcccg actggcactt     6420

catccagcac aagctgaccc gcgaggaccg cagcgacgcc aagaaccaga agtggcacct     6480

gaccgagcac gccatcgcct ccggctccgc cttgcccaag cttccgcgga gccatggctt     6540

cccgccggcg gtggcggcgc aggatgatgg cacgctgccc atgtcttgtg cccaggagag     6600

cgggatggac cgtcaccctg cagcctgtgc ttctgctagg atcaatgtgt agacacagtc     6660

tctgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc     6720

ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt     6780

ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat     6840

tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggactt gcgtagtgag     6900

tcgaataagg gcgacacaaa atttattcta aatgcataat aaatactgat aacatcttat     6960

agtttgtatt atattttgta ttatcgttga catgtataat tttgatatca aaaactgatt     7020

ttccctttat tattttcgag atttattttc ttaattctct ttaacaaact agaaatattg     7080

tatatacaaa aaatcataaa taatagatga atagtttaat tataggtgtt catcaatcga     7140

aaaagcaacg tatcttattt aaagtgcgtt gcttttttct catttataag gttaaataat     7200

tctcatatat caagcaaagt gacaggcgcc cttaaatatt ctgacaaatg ctctttccct     7260

aaactccccc cataaaaaaa cccgccgaag cgggttttta cgttatttgc ggattaacga     7320

ttactcgtta tcagaaccgc ccagggggcc cgagcttaag actggccgtc gttttacaac     7380

acagaaagag tttgtagaaa cgcaaaaagg ccatccgtca ggggccttct gcttagtttg     7440

atgcctggca gttccctact ctcgccttcc gcttcctcgc tcactgactc gctgcgctcg     7500

gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca     7560

gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac     7620

cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac     7680

aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg     7740

tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac     7800

ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat     7860

ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag     7920

cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac     7980

ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt     8040

gctacagagt tcttgaagtg gtgggctaac tacggctaca ctagaagaac agtatttggt     8100

atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc     8160

aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga     8220

aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac     8280

gacgcgcgcg taactcacgt taagggattt tggtcatgag cttgcgccgt cccgtcaagt     8340

cagcgtaatg ctctgctttt accaatgctt aatcagtgag gcacctatct cagcgatctg     8400

tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga     8460

gggcttacca tctggcccca gcgctgcgat gataccgcga gaaccacgct caccggctcc     8520

ggatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac     8580

tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc     8640

agttaatagt ttgcgcaacg ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc     8700

gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc     8760

catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt     8820

ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc     8880

atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg     8940

tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag     9000

cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat     9060

cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc     9120

atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa     9180

aaagggaata agggcgacac ggaaatgttg aatactcata ttcttccttt ttcaatatta     9240

ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa     9300

aaataaacaa ataggggtca gtgttacaac caattaacca attctgaaca ttatcgcgag     9360

cccatttata cctgaatatg gctcataaca ccccttgttt gcctggcggc agtagcgcgg     9420

tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg     9480

tggggactcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag     9540

tcgaaagact gggcctttcg cccgggctaa ttatggggtg tcgcccttat tcgactc        9597


<210>  72
<211>  9577
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  misc_feature
<222>  (1)..(9577)
<223>  Mgat1-mCherry-HDR-TI donor (sgRNA1)

<400>  72
agtcggtgtt tgcagcaaat cagggagcat catgctttgt ggagacagag gtggaaagtg       60

cccaccgtgg cccctccagc ctggccccgt gtgcctgcga ccccctcacc agccgtgatc      120

cccatcctgg tcattgcctg tgaccgcagc actgtccggc gctgcttgga taagttgttg      180

cactatcggc cctcagctga gcatttcccc atcattgtca gccaggactg cgggcacgag      240

gagacagcac aggtcattgc ttcctatggc agtgcagtca cacacatccg gcagccagac      300

ctgagtaaca tcgctgtgcc cccagaccac cgcaagttcc agggttacta caagatcgcc      360

aggcactacc gctgggcact gggccagatc ttcaacaagt tcaagttccc agcagctgtg      420

gtagtggagg acgatctgga ggtggcacca gacttctttg agtacttcca ggccacctac      480

ccactgctga gaacagaccc ctccctttgg tgtgtgtctg cttggaatga caatggcaag      540

gagcagatgg tagactcaag caaacctgag ctgctctatc gaacagactt ttttcctggc      600

cttggctggc tgctgatggc tgagctgtgg acagagctgg agcccaagtg gcccaaggcc      660

ttctgggatg actggatgcg cagacctgag cagcggaagg ggcgggcctg tattcgtcca      720

gaaatttcaa gaacgatgaa agcagcgtgt gaggctccgg tgcccgtcag tgggcagagc      780

gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct      840

agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc      900

ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca      960

acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct     1020

ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctccag tacgtgattc     1080

ttgatcccga gctggagcca ggggcgggcc ttgcgcttta ggagcccctt cgcctcgtgc     1140

ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg     1200

cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc     1260

gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caggatctgc acactggtat     1320

ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc     1380

gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg     1440

gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct     1500

ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctccagg     1560

gggctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag     1620

gaaaggggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc     1680

gtccaggcac ctcgattagt tctggagctt ttggagtacg tcgtctttag gttgggggga     1740

ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc     1800

ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat     1860

tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagacg     1920

tcatcgccac catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca     1980

tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg     2040

agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg     2100

gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct     2160

acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca     2220

agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct     2280

ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg     2340

acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc     2400

ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc     2460

actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg     2520

cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg     2580

aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt     2640

agacacagtc tctgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc     2700

ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc     2760

atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa     2820

gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggaggt     2880

ctgagtctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc     2940

agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc     3000

tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg     3060

cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat     3120

ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc     3180

cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct     3240

tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa     3300

caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac     3360

tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg     3420

cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag     3480

gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt     3540

gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg     3600

tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg     3660

catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga     3720

gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag     3780

gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat     3840

ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt     3900

tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg     3960

gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt     4020

tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc     4080

ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac     4140

gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg     4200

acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca     4260

acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa     4320

ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt     4380

atcatgtctg aggtctgagt catgggcagt tctttgatca gcatcttaag ttcatcaagc     4440

tgaaccagca gttcgtgtct ttcacccagt tggatttgtc atacttgcag cgggaggctt     4500

atgaccggga tttccttgcc cgtgtctata gtgcccccct gctacaggtg gagaaagtga     4560

ggaccaatga tcagaaggag ctgggggagg tgcgggtaca gtacactagc agagacagct     4620

tcaaggcctt tgctaaggcc ctgggtgtca tggatgacct caagtctggt gtccccagag     4680

ctggctaccg gggcgttgtc actttccagt tcaggggtcg acgtgtccac ctggcacccc     4740

cacaaacctg ggaaggctat gatcctagct ggaattagca gcacctgcct ttccctgctg     4800

gatctgcttg tcatatcatg agctgagaca ggcctgcagt ccctgagctg taccatcctg     4860

tccctgtttc cctcttgggt ctatatatct cctatttctg tgcccccctc cctccctgtg     4920

gcattctagt gcataaatca tgatgagggt tatactcctg ttgtccaggg agtcatcagg     4980

agaactattg tgtggtgtag ttgggggtat tgaacaagaa accactgtgt ggtatgggga     5040

ggcttgggct tgttggggcc aaggagtcct gagttctctg gaagggcatc gcagagagct     5100

tggcaactcg agctctcttg accaagcctg ttgaccctaa cctggctcct acgtcgctgt     5160

tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc     5220

ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc     5280

aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg     5340

actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat     5400

caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc     5460

tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta     5520

ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag     5580

cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt     5640

tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa     5700

atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga     5760

gaacccactg cttactggct tatcgaaata gtgcgatcgc caccatggcc cagtccaagc     5820

acggcctgac caaggagatg accatgaagt accgcatgga gggctgcgtg gacggccaca     5880

agttcgtgat caccggcgag ggcatcggct accccttcaa gggcaagcag gccatcaacc     5940

tgtgcgtggt ggagggcggc cccttgccct tcgccgagga catcttgtcc gccgccttca     6000

tgtacggcaa ccgcgtgttc accgagtacc cccaggacat cgtcgactac ttcaagaact     6060

cctgccccgc cggctacacc tgggaccgct ccttcctgtt cgaggacggc gccgtgtgca     6120

tctgcaacgc cgacatcacc gtgagcgtgg aggagaactg catgtaccac gagtccaagt     6180

tctacggcgt gaacttcccc gccgacggcc ccgtgatgaa gaagatgacc gacaactggg     6240

agccctcctg cgagaagatc atccccgtgc ccaagcaggg catcttgaag ggcgacgtga     6300

gcatgtacct gctgctgaag gacggtggcc gcttgcgctg ccagttcgac accgtgtaca     6360

aggccaagtc cgtgccccgc aagatgcccg actggcactt catccagcac aagctgaccc     6420

gcgaggaccg cagcgacgcc aagaaccaga agtggcacct gaccgagcac gccatcgcct     6480

ccggctccgc cttgcccaag cttccgcgga gccatggctt cccgccggcg gtggcggcgc     6540

aggatgatgg cacgctgccc atgtcttgtg cccaggagag cgggatggac cgtcaccctg     6600

cagcctgtgc ttctgctagg atcaatgtgt agacacagtc tctgtgcctt ctagttgcca     6660

gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac     6720

tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat     6780

tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca     6840

tgctggggat gcggtgggct ctatggactt gcgtagtgag tcgaataagg gcgacacaaa     6900

atttattcta aatgcataat aaatactgat aacatcttat agtttgtatt atattttgta     6960

ttatcgttga catgtataat tttgatatca aaaactgatt ttccctttat tattttcgag     7020

atttattttc ttaattctct ttaacaaact agaaatattg tatatacaaa aaatcataaa     7080

taatagatga atagtttaat tataggtgtt catcaatcga aaaagcaacg tatcttattt     7140

aaagtgcgtt gcttttttct catttataag gttaaataat tctcatatat caagcaaagt     7200

gacaggcgcc cttaaatatt ctgacaaatg ctctttccct aaactccccc cataaaaaaa     7260

cccgccgaag cgggttttta cgttatttgc ggattaacga ttactcgtta tcagaaccgc     7320

ccagggggcc cgagcttaag actggccgtc gttttacaac acagaaagag tttgtagaaa     7380

cgcaaaaagg ccatccgtca ggggccttct gcttagtttg atgcctggca gttccctact     7440

ctcgccttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc     7500

ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg     7560

aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct     7620

ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca     7680

gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct     7740

cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc     7800

gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt     7860

tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc     7920

cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc     7980

cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg     8040

gtgggctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc     8100

agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag     8160

cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga     8220

tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gacgcgcgcg taactcacgt     8280

taagggattt tggtcatgag cttgcgccgt cccgtcaagt cagcgtaatg ctctgctttt     8340

accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag     8400

ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca     8460

gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc     8520

agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt     8580

ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg     8640

ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca     8700

gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg     8760

ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca     8820

tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg     8880

tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct     8940

cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca     9000

tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca     9060

gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg     9120

tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac     9180

ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt     9240

attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca     9300

gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg     9360

gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg     9420

ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga     9480

gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg     9540

cccgggctaa ttatggggtg tcgcccttat tcgactc                              9577


<210>  73
<211>  9597
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  misc_feature
<222>  (1)..(9597)
<223>  Mgat1-mCherry-HDR-TI donor (sgRNA5)

<400>  73
agtcggtgtt cactgtgttt ccttactaag tcaggtatga tactggattt tttttttttt       60

ttttaatcag taattctatg gcagcttgac ttgagaaatg ggcgttaaat ggccttacct      120

ctccctcctg aactagatag actctggctg aaagacacga ggtgagatct gtctggtagg      180

taccaggagt tgcagttttt ctttcttttt tttttttttc ccagttctgt tccactagca      240

tctttccttg cctccttgtc atactaacat tgtttttctg aggacccatt agctcatttg      300

aagtgaagag catgctgtcc taaccctgca gtcaactgcc ttacttccct ttttccttag      360

tttctcacct gtgcctatcc tcttccctgt ggcatgtgga attattatac atgcttgaag      420

taacagttgg ttttctctct cctctcttta ggtacatggc ttcttcctag tcccagccca      480

gaagaataaa cccttagact ggggaagtga gccaggcaag ccaaaggcag ccttgagccc      540

tccccttgcc tgccctcccc tgtgggggcc aggatgctga agaagcagtc tgcagggctt      600

gtgctttggg gtgctatcct ctttgtgggc tggaatgccc tgctgctcct cttcttctgg      660

acacgcccag cccctggcag gcccccctca gatagggcta tctctgatgc ccctgccagc      720

ctcacccgtg aggtgttccg cctggctgag gacgctgaga agcagcgtgt gaggctccgg      780

tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt      840

cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt      900

gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc      960

cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg     1020

ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac     1080

ctggctccag tacgtgattc ttgatcccga gctggagcca ggggcgggcc ttgcgcttta     1140

ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg     1200

cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa     1260

aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc     1320

caggatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg     1380

tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg     1440

gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc     1500

ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg     1560

cttcccggcc ctgctccagg gggctcaaaa tggaggacgc ggcgctcggg agagcgggcg     1620

ggtgagtcac ccacacaaag gaaaggggcc tttccgtcct cagccgtcgc ttcatgtgac     1680

tccacggagt accgggcgcc gtccaggcac ctcgattagt tctggagctt ttggagtacg     1740

tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg     1800

gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt     1860

gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca     1920

tttcaggtgt cgtgaagacg tcatcgccac catggtgagc aagggcgagg aggataacat     1980

ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca     2040

cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa     2100

gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt     2160

catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct     2220

gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt     2280

gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg     2340

cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc     2400

ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct     2460

gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa     2520

gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa     2580

cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg     2640

catggacgag ctgtacaagt agacacagtc tctgtgcctt ctagttgcca gccatctgtt     2700

gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc     2760

taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt     2820

ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat     2880

gcggtgggct ctatggaggt ctgagtctgt ggaatgtgtg tcagttaggg tgtggaaagt     2940

ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca     3000

ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt     3060

agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt     3120

ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg     3180

cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt     3240

gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag     3300

gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg     3360

agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt     3420

tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc     3480

tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt     3540

gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag     3600

tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg     3660

ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag     3720

cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg     3780

atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc     3840

gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca     3900

tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc     3960

gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg     4020

ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct     4080

atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc     4140

gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg     4200

cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct     4260

ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa     4320

tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc     4380

caaactcatc aatgtatctt atcatgtctg aggtctgagt gctgttgcag caaatcaggg     4440

agcatcatgc tttgtggaga cagaggtgga aagtgcccac cgtggcccct ccagcctggc     4500

cccgtgtgcc tgcgaccccc tcaccagccg tgatccccat cctggtcatt gcctgtgacc     4560

gcagcactgt ccggcgctgc ttggataagt tgttgcacta tcggccctca gctgagcatt     4620

tccccatcat tgtcagccag gactgcgggc acgaggagac agcacaggtc attgcttcct     4680

atggcagtgc agtcacacac atccggcagc cagacctgag taacatcgct gtgcccccag     4740

accaccgcaa gttccagggt tactacaaga tcgccaggca ctaccgctgg gcactgggcc     4800

agatcttcaa caagttcaag ttcccagcag ctgtggtagt ggaggacgat ctggaggtgg     4860

caccagactt ctttgagtac ttccaggcca cctacccact gctgagaaca gacccctccc     4920

tttggtgtgt gtctgcttgg aatgacaatg gcaaggagca gatggtagac tcaagcaaac     4980

ctgagctgct ctatcgaaca gacttttttc ctggccttgg ctggctgctg atggctgagc     5040

tgtggacaga gctggagccc aagtggccca aggccttctg ggatgactgg atgcgcagac     5100

ctgagcagcg gaaggggcgg gcctgtattc gtccagaaat ttcaagaacg atgacctttg     5160

gccgtaaggg acgtcgctgt tgacattgat tattgactag ttattaatag taatcaatta     5220

cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg     5280

gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc     5340

ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa     5400

ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca     5460

atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta     5520

cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt     5580

acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg     5640

acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca     5700

actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca     5760

gagctctctg gctaactaga gaacccactg cttactggct tatcgaaata gtgcgatcgc     5820

caccatggcc cagtccaagc acggcctgac caaggagatg accatgaagt accgcatgga     5880

gggctgcgtg gacggccaca agttcgtgat caccggcgag ggcatcggct accccttcaa     5940

gggcaagcag gccatcaacc tgtgcgtggt ggagggcggc cccttgccct tcgccgagga     6000

catcttgtcc gccgccttca tgtacggcaa ccgcgtgttc accgagtacc cccaggacat     6060

cgtcgactac ttcaagaact cctgccccgc cggctacacc tgggaccgct ccttcctgtt     6120

cgaggacggc gccgtgtgca tctgcaacgc cgacatcacc gtgagcgtgg aggagaactg     6180

catgtaccac gagtccaagt tctacggcgt gaacttcccc gccgacggcc ccgtgatgaa     6240

gaagatgacc gacaactggg agccctcctg cgagaagatc atccccgtgc ccaagcaggg     6300

catcttgaag ggcgacgtga gcatgtacct gctgctgaag gacggtggcc gcttgcgctg     6360

ccagttcgac accgtgtaca aggccaagtc cgtgccccgc aagatgcccg actggcactt     6420

catccagcac aagctgaccc gcgaggaccg cagcgacgcc aagaaccaga agtggcacct     6480

gaccgagcac gccatcgcct ccggctccgc cttgcccaag cttccgcgga gccatggctt     6540

cccgccggcg gtggcggcgc aggatgatgg cacgctgccc atgtcttgtg cccaggagag     6600

cgggatggac cgtcaccctg cagcctgtgc ttctgctagg atcaatgtgt agacacagtc     6660

tctgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc     6720

ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt     6780

ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat     6840

tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggactt gcgtagtgag     6900

tcgaataagg gcgacacaaa atttattcta aatgcataat aaatactgat aacatcttat     6960

agtttgtatt atattttgta ttatcgttga catgtataat tttgatatca aaaactgatt     7020

ttccctttat tattttcgag atttattttc ttaattctct ttaacaaact agaaatattg     7080

tatatacaaa aaatcataaa taatagatga atagtttaat tataggtgtt catcaatcga     7140

aaaagcaacg tatcttattt aaagtgcgtt gcttttttct catttataag gttaaataat     7200

tctcatatat caagcaaagt gacaggcgcc cttaaatatt ctgacaaatg ctctttccct     7260

aaactccccc cataaaaaaa cccgccgaag cgggttttta cgttatttgc ggattaacga     7320

ttactcgtta tcagaaccgc ccagggggcc cgagcttaag actggccgtc gttttacaac     7380

acagaaagag tttgtagaaa cgcaaaaagg ccatccgtca ggggccttct gcttagtttg     7440

atgcctggca gttccctact ctcgccttcc gcttcctcgc tcactgactc gctgcgctcg     7500

gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca     7560

gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac     7620

cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac     7680

aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg     7740

tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac     7800

ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat     7860

ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag     7920

cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac     7980

ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt     8040

gctacagagt tcttgaagtg gtgggctaac tacggctaca ctagaagaac agtatttggt     8100

atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc     8160

aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga     8220

aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac     8280

gacgcgcgcg taactcacgt taagggattt tggtcatgag cttgcgccgt cccgtcaagt     8340

cagcgtaatg ctctgctttt accaatgctt aatcagtgag gcacctatct cagcgatctg     8400

tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga     8460

gggcttacca tctggcccca gcgctgcgat gataccgcga gaaccacgct caccggctcc     8520

ggatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac     8580

tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc     8640

agttaatagt ttgcgcaacg ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc     8700

gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc     8760

catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt     8820

ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc     8880

atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg     8940

tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag     9000

cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat     9060

cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc     9120

atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa     9180

aaagggaata agggcgacac ggaaatgttg aatactcata ttcttccttt ttcaatatta     9240

ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa     9300

aaataaacaa ataggggtca gtgttacaac caattaacca attctgaaca ttatcgcgag     9360

cccatttata cctgaatatg gctcataaca ccccttgttt gcctggcggc agtagcgcgg     9420

tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg     9480

tggggactcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag     9540

tcgaaagact gggcctttcg cccgggctaa ttatggggtg tcgcccttat tcgactc        9597


<210>  74
<211>  25
<212>  RNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(25)
<223>  6924; BB_Cas9_2A-link_fw

<400>  74
agaagcaugg acaagaaaac ccacg                                             25


<210>  75
<211>  23
<212>  RNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(23)
<223>  6925; BB_Cas9_2A-link_rv

<400>  75
agcagguggc ggcgcagcca gcc                                               23


<210>  76
<211>  27
<212>  RNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(27)
<223>  6926; eGFP_2A_2A-link_fw

<400>  76
accagcaugg agcaagggcg aggagcg                                           27


<210>  77
<211>  27
<212>  RNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(27)
<223>  6927; eGFP_2A_2A-link_rv

<400>  77
agccucgacg gggccaggac cccgacg                                           27


<210>  78
<211>  53
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(53)
<223>  7531; FUT8 gRNA3_F_Nex

<400>  78
tcgtcggcag cgtcagatgt gtataagaga cagtgccccc atgactaggg ata              53


<210>  79
<211>  55
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(55)
<223>  7532; FUT8 gRNA3_R_Nex

<400>  79
gtctcgtggg ctcggagatg tgtataagag acagtctgcg ttcgagaagc tgaaa            55


<210>  80
<211>  53
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(53)
<223>  8738; BAK1_1544257_F_Nex


<400>  80
tcgtcggcag cgtcagatgt gtataagaga cagcaaggtg ggctctccgt gat              53


<210>  81
<211>  56
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(56)
<223>  8739; BAK1_1544257_R_Nex

<400>  81
gtctcgtggg ctcggagatg tgtataagag acagcgatgc aatggtgcag tatgat           56


<210>  82
<211>  54
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(54)
<223>  8740; BAX_1345650_F_Nex

<400>  82
tcgtcggcag cgtcagatgt gtataagaga cagtgtggat actaactccc cacg             54


<210>  83
<211>  54
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(54)
<223>  8741; BAX_1345650_R_Nex

<400>  83
gtctcgtggg ctcggagatg tgtataagag acagtccctg aacctcacta cccc             54


<210>  84
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  misc_binding
<222>  (1)..(23)
<223>  FUT8_681494 (sgRNA3_F); CRISPy identification number 681494

<400>  84
gtcagacgca ctgacaaagt ggg                                               23


<210>  85
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  misc_binding
<222>  (1)..(23)
<223>  BAK-1_1544257; CRISPy identification number 1544257

<400>  85
ggaagccggt caaaccacgt tgg                                               23


<210>  86
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  misc_binding
<222>  (1)..(23)
<223>  BAX_1345650; CRISPy identification number 1345650

<400>  86
gctgatggca acttcaactg ggg                                               23


<210>  87
<211>  54
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(54)
<223>  BAK-1_1544257_fwd

<400>  87
ggaaaggacg aaacaccgga agccggtcaa accacgtgtt ttagagctag aaat             54


<210>  88
<211>  53
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(53)
<223>  BAK-1_1544257_rev

<400>  88
ctaaaacacg tggtttgacc ggcttccggt gtttcgtcct ttccacaaga tat              53


<210>  89
<211>  54
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(54)
<223>  BAX_1345650_fwd

<400>  89
ggaaaggacg aaacaccgct gatggcaact tcaactggtt ttagagctag aaat             54


<210>  90
<211>  54
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(54)
<223>  BAX_1345650_rev

<400>  90
cctaaaacca gttgaagttg ccatcagcgg tgtttcgtcc tttccacaag atat             54


<210>  91
<211>  10063
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  misc_feature
<222>  (1)..(10063)
<223>  GFP_2A_Cas9 vector

<400>  91
ctcatgacca aaatccctta acgtgagtta cgcgcgcgtc gttccactga gcgtcagacc       60

ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct      120

tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa      180

ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag      240

tgtagccgta gttagcccac cacttcaaga actctgtagc accgcctaca tacctcgctc      300

tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg      360

actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca      420

cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat      480

gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg      540

tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc      600

ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc      660

ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc      720

cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg      780

cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga      840

gcgaggaagc ggaaggcgag agtagggaac tgccaggcat caaactaagc agaaggcccc      900

tgacggatgg cctttttgcg tttctacaaa ctctttctgt gttgtaaaac gacggccagt      960

cttaagctcg ggccccctgg gcggttctga taacgagtaa tcgttaatcc gcaaataacg     1020

taaaaacccg cttcggcggg tttttttatg gggggagttt agggaaagag catttgtcag     1080

aatatttaag ggcgcctgtc actttgcttg atatatgaga attatttaac cttataaatg     1140

agaaaaaagc aacgcacttt aaataagata cgttgctttt tcgattgatg aacacctata     1200

attaaactat tcatctatta tttatgattt tttgtatata caatatttct agtttgttaa     1260

agagaattaa gaaaataaat ctcgaaaata ataaagggaa aatcagtttt tgatatcaaa     1320

attatacatg tcaacgataa tacaaaatat aatacaaact ataagatgtt atcagtattt     1380

attatcattt agaataaatt ttgtgtcgcc cttaattgtg agcggataac aattacgagc     1440

ttcatgcaca gtggcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg     1500

ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc     1560

cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca     1620

tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg     1680

cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg     1740

acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt     1800

ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca     1860

tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg     1920

tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact     1980

ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag     2040

ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact     2100

ataggggaag ctggctagcg ccgccaccat gcatgtgagc aagggcgagg agctgttcac     2160

cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt     2220

gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac     2280

caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca     2340

gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc     2400

cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg     2460

cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga     2520

cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa     2580

cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca     2640

caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg     2700

cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa     2760

agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat     2820

cactctcggc atggacgagc tgtacaagcc taggggcagt ggagagggca gaggaagtct     2880

gctaacatgc ggtgacgtcg aggagaatcc tggcccacga tcgagaagca tggacaagaa     2940

atactccatc ggccttgata tcgggaccaa cagcgtgggt tgggccgtga tcactgacga     3000

atacaaagtg ccatcgaaaa agtttaaggt gctgggcaat actgacagac atagcatcaa     3060

gaaaaacctg attggagccc tcctgttcga ctccggggaa accgccgaag ccactcgcct     3120

gaaacggacc gcacgccgga ggtacactcg ccggaagaat agaatctgct accttcaaga     3180

gatctttagc aacgagatgg ccaaagtgga cgattcgttc ttccaccggc tggaggagtc     3240

attcctggtc gaagaggaca aaaagcatga gagacatccg atcttcggta acattgtgga     3300

tgaggtcgcc taccacgaga agtaccccac tatctaccat ttgcgcaaga agttggtcga     3360

ctcgactgat aaggccgacc tcagactcat ctacctcgct ttggcacaca tgatcaagtt     3420

taggggtcac ttcctgattg aaggggatct gaacccagac aactcggatg tggataaact     3480

gtttatccag ctggtccaga cttacaatca gctctttgag gagaacccga tcaatgcctc     3540

cggcgtggat gctaaggcaa tcctgtccgc acgcttgtca aagagccggc ggctcgaaaa     3600

tctgatcgca cagcttccgg gagaaaagaa aaacggactg tttggaaacc ttatcgccct     3660

ctcgctggga ctcactccta acttcaaatc caattttgat ttggccgagg atgccaagct     3720

gcagctgtcg aaggacacct acgacgatga cctcgacaat cttctggccc agatcgggga     3780

ccagtacgcc gatctgttcc tcgcggccaa aaacctgtca gacgcaatct tgctgagcga     3840

tatcttgagg gtcaataccg aaatcactaa ggctccattg tcagcatcga tgatcaagag     3900

gtacgatgaa caccatcagg acctgaccct gctcaaggca ctggtgcggc agcaactgcc     3960

ggaaaagtac aaggagatct tcttcgatca aagcaagaat gggtacgcag ggtacatcga     4020

tggtggagca tcacaagaag agttctacaa attcatcaag cccatccttg aaaagatgga     4080

cgggacggaa gaactgctgg tgaagctgaa tcgggaagat ctgctgcgga agcagcgcac     4140

cttcgacaat ggttccatcc cacaccaaat ccatctcggc gaactgcacg cgatccttcg     4200

ccggcaggaa gatttctacc cgttcttgaa ggataacaga gaaaagatcg agaaaatcct     4260

gacctttaga atcccgtact acgtgggccc gttggctcgc ggaaactcaa gattcgcctg     4320

gatgactaga aaatccgaag aaacgattac cccgtggaac tttgaagagg tcgtcgacaa     4380

gggagcctcg gcacagtcgt tcatcgaacg gatgaccaat ttcgacaaga acctccccaa     4440

cgaaaaggtg ctcccgaaac actcgctgct gtatgagtac ttcacggtct acaacgagct     4500

gaccaaggtg aagtacgtga ccgaaggaat gcggaagccc gcttttctgt ccggtgaaca     4560

gaaaaaggcc atcgtggacc tcctcttcaa gactaacaga aaggtgaccg tgaaacagct     4620

taaagaggac tacttcaaga agatcgagtg ttttgactcc gtggaaatct cgggagtgga     4680

ggataggttt aacgcttcgc tggggaccta ccatgacctt ctcaagatca tcaaggataa     4740

ggatttcctg gacaacgaag aaaacgagga catccttgag gacatcgtgt tgaccttgac     4800

cctgtttgag gatcgggaaa tgatcgagga gagactgaaa acttacgcgc atcttttcga     4860

cgacaaagtg atgaagcagc tgaaaagaag aagatacact ggctggggac ggctgtcgag     4920

gaaactgatt aacggaatta gggataaaca aagcggaaag actatcctcg attttctcaa     4980

gtcggacggc ttcgccaatc ggaactttat gcagctcatc cacgacgata gcctgacctt     5040

caaggaagat atccaaaaag cccaagtgtc cggccaggga gatagcttgc acgagcacat     5100

tgctaacctg gccggttcac cagccattaa gaagggaatc ctccaaaccg tgaaggtggt     5160

cgacgaactc gtcaaggtga tgggccgcca taagccagag aacatcgtga tcgagatggc     5220

tcgcgaaaat cagactactc agaagggtca aaagaactcc cgcgagcgca tgaagcgcat     5280

cgaagaagga attaaggagc tgggctcaca aatcctcaaa gaacatcctg tcgagaacac     5340

ccaacttcag aacgagaaac tgtacctcta ctatctccaa aatggccggg acatgtacgt     5400

cgatcaggaa ttggatatca accgcctctc agactacgac gtggatcata tcgtgccgca     5460

gtcgtttctc aaagatgata gcatcgacaa caaggtcctg acgaggtcgg ataagaaccg     5520

cgggaaatcc gacaatgtgc cttcggaaga ggtggtgaaa aagatgaaga actactggag     5580

gcaattgctt aatgccaaac tgatcaccca gcgcaaattc gacaacctta ctaaggccga     5640

gcgcggagga ctgtccgaac tcgataaggc ggggttcatc aaaagacaat tggtcgaaac     5700

tcggcaaatt accaagcatg tcgctcagat cctcgactcg cgcatgaaca ctaagtatga     5760

tgagaacgac aagctcattc gcgaagtgaa agtgattacc ctcaaatcaa agctggtgtc     5820

ggacttcaga aaagactttc aattctacaa ggtgcgggag attaacaact accaccacgc     5880

gcacgacgcc tacctcaatg cagtggtggg aaccgccctc atcaagaagt atccaaagct     5940

cgaaagcgag ttcgtgtacg gggactataa agtgtacgat gtgcggaaaa tgatcgcaaa     6000

gagcgagcaa gagatcggca aagcaacggc caaatacttc ttctacagca acattatgaa     6060

ctttttcaag accgagatta ccctggcgaa cggagaaatc cggaagcggc ctttgatcga     6120

aacgaatggg gaaactggag aaatcgtgtg ggacaagggc agagactttg cgaccgtgag     6180

aaaggtgctg tccatgccac aagtcaacat cgtgaagaaa actgaagtgc agactggagg     6240

attttccaag gagtcaatcc ttccgaagcg caacagcgac aagctgatcg ccaggaagaa     6300

ggattgggac cccaagaagt acggtggttt tgattcacct actgtcgctt acagcgtgct     6360

ggtggtggcc aaggtggaaa aggggaagtc aaagaaattg aagtcggtga aggaattgct     6420

ggggattact atcatggaga ggagcagctt cgaaaagaat cccatcgact tcttggaggc     6480

caagggatac aaagaggtga agaaagacct gatcattaag ctgccgaagt actccttgtt     6540

cgaactggaa aacggcagaa agcggatgct ggcctccgcc ggagagctgc agaagggtaa     6600

cgagctggct ctgcccagca aatacgtgaa tttcctctac ctggcctccc attacgagaa     6660

gctcaaggga agcccggagg ataatgagca aaaacaactg ttcgtcgagc agcacaagca     6720

ctacctcgac gagatcatcg aacaaatctc cgagttctcg aagcgggtga ttctggccga     6780

cgcaaacctt gataaagtcc tcagcgccta caacaagcac cgcgacaaac cgatcagaga     6840

acaagctgag aacatcatcc acctgttcac gctgactaac ttgggagcgc ctgccgcctt     6900

caagtacttc gacaccacta tcgatcggaa acggtacact tccactaagg aagtgctgga     6960

cgcaaccctg atccatcagt ccatcaccgg actttatgag actcggatcg atctcagcca     7020

gcttggcggt gattcaagag ctgacccgaa gaagaagcgc aaggtctaga aaatcagcct     7080

cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga     7140

ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcacaac     7200

actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta     7260

ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg     7320

tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg     7380

catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt     7440

atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc     7500

ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt     7560

atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc     7620

ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga     7680

tctgatcagc acgtgatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg     7740

atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt     7800

gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat     7860

ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg     7920

gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca     7980

cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc     8040

gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca     8100

ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct     8160

gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg     8220

caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg     8280

cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt     8340

gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg     8400

aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag     8460

cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat     8520

cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca     8580

atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc     8640

gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact     8700

cgtccgaggg caaaggaata gcacgtgcta cgagatttcg attccaccgc cgccttctat     8760

gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg     8820

gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac     8880

aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt     8940

tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc     9000

tagagcttgg cgtaatcatg gtcattacca atgcttaatc agtgaggcac ctatctcagc     9060

gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat     9120

acgggagggc ttaccatctg gccccagcgc tgcgatgata ccgcgagaac cacgctcacc     9180

ggctccggat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc     9240

tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag     9300

ttcgccagtt aatagtttgc gcaacgttgt tgccatcgct acaggcatcg tggtgtcacg     9360

ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg     9420

atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag     9480

taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt     9540

catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga     9600

atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc     9660

acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc     9720

aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc     9780

ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc     9840

cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatattct tcctttttca     9900

atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat     9960

ttagaaaaat aaacaaatag gggtcagtgt tacaaccaat taaccaattc tgaacattat    10020

cgcgagccca tttatacctg aatatggctc ataacacccc ttg                      10063


<210>  92
<211>  30
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(30)
<223>  ro125; COSMC 5' arm_750bp_LA_fwd

<400>  92
agtcggtgug taatccatgg aggagtttct                                        30


<210>  93
<211>  30
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(30)
<223>  pro126; COSMC 5' arm_750bp_LB_rev

<400>  93
acgctgctua aggtctccag attttacagt                                        30


<210>  94
<211>  26
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(26)
<223>  pro121; EF-1a_LB_fwd

<400>  94
aagcagcgug tgaggctccg gtgccc                                            26


<210>  95
<211>  29
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(29)
<223>  pro122; EF-1a_LC_rev

<400>  95
atgacgtcut cacgacacct gaaatggaa                                         29


<210>  96
<211>  29
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(29)
<223>  pro123; kozak_co-EPO_LC_fwd

<400>  96
agacgtcauc gccaccatgg gagtgcacg                                         29


<210>  97
<211>  30
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(30)
<223>  pro038; BGH pA_LD_rev

<400>  97
actcagaccu ccatagagcc caccgcatcc                                        30


<210>  98
<211>  28
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(28)
<223>  pro603; kozak_Enbrel_LC_fwd

<400>  98
agacgtcaua gcaccatggc gcccgtcg                                          28


<210>  99
<211>  29
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(29)
<223>  pro602; kozak_Rituximab HC_LC_fwd

<400>  99
agacgtcaug acaccatggg ctggtcctg                                         29


<210>  100
<211>  28
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(28)
<223>  pro064; BGH pA_O2_rev

<400>  100
atcgcacucc atagagccca ccgcatcc                                          28


<210>  101
<211>  25
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(25)
<223>  pro604; EF-1a_O2_fwd

<400>  101
agtgcgaugt gaggctccgg tgccc                                             25


<210>  102
<211>  31
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(31)
<223>  pro127; COSMC 3' arm_750bp_LD_fwd

<400>  102
aggtctgagu gattgtctta agcatagagt c                                      31


<210>  103
<211>  29
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(29)
<223>  pro128; COSMC 3' arm_750bp_O1_ref

<400>  103
agcgacgucc tcatttgcat atatttgaa                                         29


<210>  104
<211>  34
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(34)
<223>  pro023; pJ204 backbone_O5_fwd

<400>  104
acttgcguag tgagtcgaat aagggcgaca caaa                                   34


<210>  105
<211>  33
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  primer_bind
<222>  (1)..(33)
<223>  pro043; pJ204 backbone_LA_rev

<400>  105
acaccgacug agtcgaataa gggcgacacc cca                                    33


<210>  106
<211>  7904
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence

<400>  106
agtcggtgtg taatccatgg aggagtttct ataatgttgc agtttctacc taatggtgac       60

caaatgccag tgaaaggatt gtaagagtac ttgtcacata tactactcac ctcatttcaa      120

gaatgtggac ctgcttttaa acattaagag caaatcgtaa ttatataaga aataagcaaa      180

tgaaactatt agactgtttg aaaagtcttt ttctttacag gaaaaatgct ttcagaaagc      240

agttcatttt tgaaaggtgt gatgcttgga agcatcttct atgccttgat cactacgcta      300

ggccacatta ggattgggca cagaaacagg acacaccacc atgagcatca ccacctgcaa      360

gctcctaaca aagaagatat ctcgaaaatc tcagcggctg agcgcatgga gctcagtaag      420

agcttccggg tatactgtat agttcttgta aaacccaaag atgtgagtct ttgggctgca      480

gtgaaggaga cttggaccaa acactgtgac aaagcagagt tcttcagttc tgaaaatgtt      540

aaagtgtttg agtcaattaa cgtggacact gatgacatgt ggttgatgat gaggaaagct      600

tataaatatg cctttgataa atacaaagag cagtacaact ggttcttcct tgcacgcccc      660

agtacttttg ctgtgattga aaatctaaaa tattttttgt taaaaaagga tccatcgcag      720

cctttctatc taggacacac tgtaaaatct ggagacctta agcagcgtgt gaggctccgg      780

tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt      840

cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt      900

gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc      960

cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg     1020

ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac     1080

ctggctccag tacgtgattc ttgatcccga gctggagcca ggggcgggcc ttgcgcttta     1140

ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg     1200

cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa     1260

aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc     1320

caggatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg     1380

tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg     1440

gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc     1500

ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg     1560

cttcccggcc ctgctccagg gggctcaaaa tggaggacgc ggcgctcggg agagcgggcg     1620

ggtgagtcac ccacacaaag gaaaggggcc tttccgtcct cagccgtcgc ttcatgtgac     1680

tccacggagt accgggcgcc gtccaggcac ctcgattagt tctggagctt ttggagtacg     1740

tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg     1800

gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt     1860

gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca     1920

tttcaggtgt cgtgaagacg tcatcgccac catgggagtg cacgagtgtc ctgcttggct     1980

gtggctgctg ctgtccctgc tgtctctgcc tctgggactg cctgtgctgg gcgctcctcc     2040

tagactgatc tgcgactccc gggtgctgga aagatacctg ctggaagcca aagaggccga     2100

gaacatcacc accggctgcg ccgagcactg ctccctgaac gagaatatca ccgtgcccga     2160

caccaaagtg aacttctacg cctggaagcg gatggaagtg ggccagcagg ctgtggaagt     2220

gtggcaggga ctggctctgc tgagcgaggc tgtgctgaga ggacaggccc tgctcgtgaa     2280

ctcctcccag ccttgggaac ccctgcagct gcacgtggac aaggctgtgt ccggcctgag     2340

atccctgacc accctgctga gagcactggg agcccagaaa gaggccatct ctccacctga     2400

cgccgcctct gctgctcctc tgagaaccat caccgccgac accttcagaa agctgttccg     2460

ggtgtactcc aacttcctgc ggggcaagct gaagctgtac accggcgagg cttgccggac     2520

cggcgataga tgagaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg     2580

gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt     2640

ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta     2700

ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg     2760

ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc     2820

ggtgggctct atggaggtct gagtgattgt cttaagcata gagtcaatga aaagactcaa     2880

cagccttctc agtgttccgg aaaagtgtcc tgaacaaggt gggatgattt ggaagatatc     2940

tgaagataag cagctagcag tctgcctgaa atatgctgga gtatttgcgg aaaatgcgga     3000

agacgctgat agaaaagatg tatttaatac caaatctgtt gggcttttca ttaaagaggc     3060

catgtctaac cacccgaacc aggtagtaga aggatgctgt tccaatatgg ctgtcacttt     3120

taatggacta actcctaatc agatgcatgt gatgatgtat ggggtgtacc ggcttagggc     3180

ctttggacat gttttcaacg atgcgttggt tttcttacct ccaaacggtt ctgataatga     3240

ctgacaaaaa gcaagagcat gcatttggta accacattaa gacatgttat gctttctaat     3300

cgataatgca tctaacacag tagtgtgttt cttttcctta tctggtcaca ttgaagtcta     3360

cttgtacatt ttcaaatgga atggtatttt tttcccttaa atcatttgtg agaaatttta     3420

atgtgttaga aataaatgtt ttaagaatag caattttgca aataatgtat ttataaatat     3480

tatatttatg tgataaagac caaattatag acattaaaat ctgtgatgta tctttgccta     3540

ttgattttaa atgtttaatg tatcttttta gatttcaaat atatgcaaat gaggacgtcg     3600

ctgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca     3660

tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc     3720

gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat     3780

agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt     3840

acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc     3900

cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta     3960

cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg     4020

atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt     4080

gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac     4140

gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac     4200

tagagaaccc actgcttact ggcttatcga aatagtgcga tcgccaccat ggtgagcaag     4260

ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag     4320

ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag     4380

ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac     4440

atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc     4500

cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc     4560

gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc     4620

tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag     4680

accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc     4740

gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc     4800

acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg     4860

gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc     4920

cgccactcca ccggcggcat ggacgagctg tacaagtaga cacagtctct gtgccttcta     4980

gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca     5040

ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc     5100

attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata     5160

gcaggcatgc tggggatgcg gtgggctcta tggacttgcg tagtgagtcg aataagggcg     5220

acacaaaatt tattctaaat gcataataaa tactgataac atcttatagt ttgtattata     5280

ttttgtatta tcgttgacat gtataatttt gatatcaaaa actgattttc cctttattat     5340

tttcgagatt tattttctta attctcttta acaaactaga aatattgtat atacaaaaaa     5400

tcataaataa tagatgaata gtttaattat aggtgttcat caatcgaaaa agcaacgtat     5460

cttatttaaa gtgcgttgct tttttctcat ttataaggtt aaataattct catatatcaa     5520

gcaaagtgac aggcgccctt aaatattctg acaaatgctc tttccctaaa ctccccccat     5580

aaaaaaaccc gccgaagcgg gtttttacgt tatttgcgga ttaacgatta ctcgttatca     5640

gaaccgccca gggggcccga gcttaagact ggccgtcgtt ttacaacaca gaaagagttt     5700

gtagaaacgc aaaaaggcca tccgtcaggg gccttctgct tagtttgatg cctggcagtt     5760

ccctactctc gccttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc     5820

ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata     5880

acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg     5940

cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct     6000

caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa     6060

gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc     6120

tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt     6180

aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg     6240

ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg     6300

cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct     6360

tgaagtggtg ggctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc     6420

tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg     6480

ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc     6540

aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgac gcgcgcgtaa     6600

ctcacgttaa gggattttgg tcatgagctt gcgccgtccc gtcaagtcag cgtaatgctc     6660

tgcttttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca     6720

tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct     6780

ggccccagcg ctgcgatgat accgcgagaa ccacgctcac cggctccgga tttatcagca     6840

ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc     6900

atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg     6960

cgcaacgttg ttgccatcgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct     7020

tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa     7080

aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta     7140

tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc     7200

ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg     7260

agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa     7320

gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg     7380

agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc     7440

accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg     7500

gcgacacgga aatgttgaat actcatattc ttcctttttc aatattattg aagcatttat     7560

cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata     7620

ggggtcagtg ttacaaccaa ttaaccaatt ctgaacatta tcgcgagccc atttatacct     7680

gaatatggct cataacaccc cttgtttgcc tggcggcagt agcgcggtgg tcccacctga     7740

ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggactcccca     7800

tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg     7860

cctttcgccc gggctaatta tggggtgtcg cccttattcg actc                      7904


<210>  107
<211>  8794
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  misc_feature
<222>  (1)..(8794)
<223>  COSMC-coEPO-HDR-TI; donor DNA plasmid with EPO

<400>  107
agtcggtgtg taatccatgg aggagtttct ataatgttgc agtttctacc taatggtgac       60

caaatgccag tgaaaggatt gtaagagtac ttgtcacata tactactcac ctcatttcaa      120

gaatgtggac ctgcttttaa acattaagag caaatcgtaa ttatataaga aataagcaaa      180

tgaaactatt agactgtttg aaaagtcttt ttctttacag gaaaaatgct ttcagaaagc      240

agttcatttt tgaaaggtgt gatgcttgga agcatcttct atgccttgat cactacgcta      300

ggccacatta ggattgggca cagaaacagg acacaccacc atgagcatca ccacctgcaa      360

gctcctaaca aagaagatat ctcgaaaatc tcagcggctg agcgcatgga gctcagtaag      420

agcttccggg tatactgtat agttcttgta aaacccaaag atgtgagtct ttgggctgca      480

gtgaaggaga cttggaccaa acactgtgac aaagcagagt tcttcagttc tgaaaatgtt      540

aaagtgtttg agtcaattaa cgtggacact gatgacatgt ggttgatgat gaggaaagct      600

tataaatatg cctttgataa atacaaagag cagtacaact ggttcttcct tgcacgcccc      660

agtacttttg ctgtgattga aaatctaaaa tattttttgt taaaaaagga tccatcgcag      720

cctttctatc taggacacac tgtaaaatct ggagacctta agcagcgtgt gaggctccgg      780

tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt      840

cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt      900

gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc      960

cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg     1020

ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac     1080

ctggctccag tacgtgattc ttgatcccga gctggagcca ggggcgggcc ttgcgcttta     1140

ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg     1200

cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa     1260

aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc     1320

caggatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg     1380

tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg     1440

gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc     1500

ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg     1560

cttcccggcc ctgctccagg gggctcaaaa tggaggacgc ggcgctcggg agagcgggcg     1620

ggtgagtcac ccacacaaag gaaaggggcc tttccgtcct cagccgtcgc ttcatgtgac     1680

tccacggagt accgggcgcc gtccaggcac ctcgattagt tctggagctt ttggagtacg     1740

tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg     1800

gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt     1860

gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca     1920

tttcaggtgt cgtgaagacg tcatagcacc atggcgcccg tcgccgtctg ggccgcgctg     1980

gccgtcggac tggagctctg ggctgcggcg cacgccttgc ccgcccaggt ggcatttaca     2040

ccctacgccc cggagcccgg gagcacatgc cggctcagag aatactatga ccagacagct     2100

cagatgtgct gcagcaaatg ctcgccgggc caacatgcaa aagtcttctg taccaagacc     2160

tcggacaccg tgtgtgactc ctgtgaggac agcacataca cccagctctg gaactgggtt     2220

cccgagtgct tgagctgtgg ctcccgctgt agctctgacc aggtggaaac tcaagcctgc     2280

actcgggaac agaaccgcat ctgcacctgc aggcccggct ggtactgcgc gctgagcaag     2340

caggaggggt gccggctgtg cgcgccgctg cgcaagtgcc gcccgggctt cggcgtggcc     2400

agaccaggaa ctgaaacatc agacgtggtg tgcaagccct gtgccccggg gacgttctcc     2460

aacacgactt catccacgga tatttgcagg ccccaccaga tctgtaacgt ggtggccatc     2520

cctgggaatg caagcatgga tgcagtctgc acgtccacgt cccccacccg gagtatggcc     2580

ccaggggcag tacacttacc ccagccagtg tccacacgat cccaacacac gcagccaact     2640

ccagaaccca gcactgctcc aagcacctcc ttcctgctcc caatgggccc cagcccccca     2700

gctgaaggga gcactggcga cgagcccaaa tcttgtgaca aaactcacac atgcccaccg     2760

tgcccagcac ctgaactcct ggggggaccg tcagtcttcc tcttcccccc aaaacccaag     2820

gacaccctca tgatctcccg gacccctgag gtcacatgcg tggtggtgga cgtgagccac     2880

gaagaccctg aggtcaagtt caactggtac gtggacggcg tggaggtgca taatgccaag     2940

acaaagccgc gggaggagca gtacaacagc acgtaccgtg tggtcagcgt cctcaccgtc     3000

ctgcaccagg actggctgaa tggcaaggag tacaagtgca aggtctccaa caaagccctc     3060

ccagccccca tcgagaaaac catctccaaa gccaaagggc agccccgaga accacaggtg     3120

tacaccctgc ccccatcccg ggaggagatg accaagaacc aggtcagcct gacctgcctg     3180

gtcaaaggct tctatcccag cgacatcgcc gtggagtggg agagcaatgg gcagccggag     3240

aacaactaca agaccacgcc tcccgtgctg gactccgacg gctccttctt cctctacagc     3300

aagctcaccg tggacaagag caggtggcag caggggaacg tcttctcatg ctccgtgatg     3360

catgaggctc tgcacaacca ctacacacag aagagcctct ccctgtctcc gggtaaatga     3420

taagaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg gcccgtttaa     3480

acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc     3540

cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag     3600

gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag     3660

gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct     3720

atggaggtct gagtgattgt cttaagcata gagtcaatga aaagactcaa cagccttctc     3780

agtgttccgg aaaagtgtcc tgaacaaggt gggatgattt ggaagatatc tgaagataag     3840

cagctagcag tctgcctgaa atatgctgga gtatttgcgg aaaatgcgga agacgctgat     3900

agaaaagatg tatttaatac caaatctgtt gggcttttca ttaaagaggc catgtctaac     3960

cacccgaacc aggtagtaga aggatgctgt tccaatatgg ctgtcacttt taatggacta     4020

actcctaatc agatgcatgt gatgatgtat ggggtgtacc ggcttagggc ctttggacat     4080

gttttcaacg atgcgttggt tttcttacct ccaaacggtt ctgataatga ctgacaaaaa     4140

gcaagagcat gcatttggta accacattaa gacatgttat gctttctaat cgataatgca     4200

tctaacacag tagtgtgttt cttttcctta tctggtcaca ttgaagtcta cttgtacatt     4260

ttcaaatgga atggtatttt tttcccttaa atcatttgtg agaaatttta atgtgttaga     4320

aataaatgtt ttaagaatag caattttgca aataatgtat ttataaatat tatatttatg     4380

tgataaagac caaattatag acattaaaat ctgtgatgta tctttgccta ttgattttaa     4440

atgtttaatg tatcttttta gatttcaaat atatgcaaat gaggacgtcg ctgttgacat     4500

tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat     4560

atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac     4620

ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc     4680

cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg     4740

tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat     4800

tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc     4860

atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt     4920

gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac     4980

caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc     5040

ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc     5100

actgcttact ggcttatcga aatagtgcga tcgccaccat ggtgagcaag ggcgaggagg     5160

ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga     5220

acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga     5280

ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc     5340

ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact     5400

tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg     5460

gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga     5520

agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct     5580

gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc     5640

agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg     5700

ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct     5760

cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca     5820

ccggcggcat ggacgagctg tacaagtaga cacagtctct gtgccttcta gttgccagcc     5880

atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt     5940

cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct     6000

ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc     6060

tggggatgcg gtgggctcta tggacttgcg tagtgagtcg aataagggcg acacaaaatt     6120

tattctaaat gcataataaa tactgataac atcttatagt ttgtattata ttttgtatta     6180

tcgttgacat gtataatttt gatatcaaaa actgattttc cctttattat tttcgagatt     6240

tattttctta attctcttta acaaactaga aatattgtat atacaaaaaa tcataaataa     6300

tagatgaata gtttaattat aggtgttcat caatcgaaaa agcaacgtat cttatttaaa     6360

gtgcgttgct tttttctcat ttataaggtt aaataattct catatatcaa gcaaagtgac     6420

aggcgccctt aaatattctg acaaatgctc tttccctaaa ctccccccat aaaaaaaccc     6480

gccgaagcgg gtttttacgt tatttgcgga ttaacgatta ctcgttatca gaaccgccca     6540

gggggcccga gcttaagact ggccgtcgtt ttacaacaca gaaagagttt gtagaaacgc     6600

aaaaaggcca tccgtcaggg gccttctgct tagtttgatg cctggcagtt ccctactctc     6660

gccttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt     6720

atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa     6780

gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc     6840

gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag     6900

gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt     6960

gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg     7020

aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg     7080

ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg     7140

taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac     7200

tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg     7260

ggctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt     7320

taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg     7380

tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc     7440

tttgatcttt tctacggggt ctgacgctca gtggaacgac gcgcgcgtaa ctcacgttaa     7500

gggattttgg tcatgagctt gcgccgtccc gtcaagtcag cgtaatgctc tgcttttacc     7560

aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg     7620

cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagcg     7680

ctgcgatgat accgcgagaa ccacgctcac cggctccgga tttatcagca ataaaccagc     7740

cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta     7800

ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg     7860

ttgccatcgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct     7920

ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta     7980

gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg     8040

ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga     8100

ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt     8160

gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca     8220

ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt     8280

cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt     8340

ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga     8400

aatgttgaat actcatattc ttcctttttc aatattattg aagcatttat cagggttatt     8460

gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggtcagtg     8520

ttacaaccaa ttaaccaatt ctgaacatta tcgcgagccc atttatacct gaatatggct     8580

cataacaccc cttgtttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg     8640

aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggactcccca tgcgagagta     8700

gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgccc     8760

gggctaatta tggggtgtcg cccttattcg actc                                 8794


<210>  108
<211>  10925
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  misc_feature
<222>  (1)..(10925)
<223>  COSMC-coEnbrel-HDR-TI; Donor DNA plasmid with Enbrel

<400>  108
agtcggtgtg taatccatgg aggagtttct ataatgttgc agtttctacc taatggtgac       60

caaatgccag tgaaaggatt gtaagagtac ttgtcacata tactactcac ctcatttcaa      120

gaatgtggac ctgcttttaa acattaagag caaatcgtaa ttatataaga aataagcaaa      180

tgaaactatt agactgtttg aaaagtcttt ttctttacag gaaaaatgct ttcagaaagc      240

agttcatttt tgaaaggtgt gatgcttgga agcatcttct atgccttgat cactacgcta      300

ggccacatta ggattgggca cagaaacagg acacaccacc atgagcatca ccacctgcaa      360

gctcctaaca aagaagatat ctcgaaaatc tcagcggctg agcgcatgga gctcagtaag      420

agcttccggg tatactgtat agttcttgta aaacccaaag atgtgagtct ttgggctgca      480

gtgaaggaga cttggaccaa acactgtgac aaagcagagt tcttcagttc tgaaaatgtt      540

aaagtgtttg agtcaattaa cgtggacact gatgacatgt ggttgatgat gaggaaagct      600

tataaatatg cctttgataa atacaaagag cagtacaact ggttcttcct tgcacgcccc      660

agtacttttg ctgtgattga aaatctaaaa tattttttgt taaaaaagga tccatcgcag      720

cctttctatc taggacacac tgtaaaatct ggagacctta agcagcgtgt gaggctccgg      780

tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt      840

cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt      900

gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc      960

cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg     1020

ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac     1080

ctggctccag tacgtgattc ttgatcccga gctggagcca ggggcgggcc ttgcgcttta     1140

ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg     1200

cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa     1260

aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc     1320

caggatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg     1380

tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg     1440

gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc     1500

ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg     1560

cttcccggcc ctgctccagg gggctcaaaa tggaggacgc ggcgctcggg agagcgggcg     1620

ggtgagtcac ccacacaaag gaaaggggcc tttccgtcct cagccgtcgc ttcatgtgac     1680

tccacggagt accgggcgcc gtccaggcac ctcgattagt tctggagctt ttggagtacg     1740

tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg     1800

gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt     1860

gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca     1920

tttcaggtgt cgtgaagacg tcatgacacc atgggctggt cctgcatcat cctgtttctg     1980

gtggctaccg ccaccggcgt gcactctcag gtgcagctgc agcagcctgg cgccgagctc     2040

gtgaaacctg gcgcctccgt gaagatgtcc tgcaaggcct ccggctacac cttcaccagc     2100

tacaacatgc actgggtcaa gcagaccccc ggcagaggcc tggaatggat cggcgctatc     2160

taccccggca acggcgacac ctcctacaac cagaagttca agggcaaggc caccctgacc     2220

gccgacaagt cctcttccac cgcctacatg cagctgtcct ccctgacctc cgaggactcc     2280

gccgtgtact actgcgcccg gtctacctac tacggcggcg actggtactt caacgtgtgg     2340

ggcgctggca ccaccgtgac cgtgtctgct gcttctacca agggcccctc cgtgttccct     2400

ctggcccctt ccagcaagtc tacctctggc ggcacagccg ctctgggctg cctcgtgaag     2460

gactacttcc ccgagcccgt gacagtgtcc tggaactctg gcgctctgac cagcggagtg     2520

cacaccttcc ctgctgtgct gcagtcctcc ggcctgtact ccctgtccag cgtcgtgact     2580

gtgccctcca gctctctggg cacccagacc tacatctgca acgtgaacca caagccctcc     2640

aacaccaagg tggacaagaa ggtggaaccc aagtcctgcg acaagaccca cacctgtccc     2700

ccttgtcctg cccctgaact gctgggcgga cccagcgtgt tcctgttccc cccaaagccc     2760

aaggataccc tgatgatctc ccggaccccc gaagtgacct gcgtggtggt ggatgtgtcc     2820

cacgaggacc ctgaagtgaa gttcaattgg tacgtggacg gcgtggaagt gcacaacgcc     2880

aagaccaagc ctagagagga acagtacaac tccacctacc gggtggtgtc cgtgctgacc     2940

gtgctgcacc aggattggct gaacggcaaa gagtacaagt gcaaggtgtc caacaaggcc     3000

ctgcctgccc ccatcgaaaa gaccatctcc aaggccaagg gccagccccg ggaaccccag     3060

gtgtacacac tgccccctag cagggacgag ctgaccaaga accaggtgtc cctgacatgc     3120

ctcgtgaaag gcttctaccc ctccgatatc gccgtggaat gggagtccaa cggccagcct     3180

gagaacaact acaagaccac cccccctgtg ctggactccg acggctcatt cttcctgtac     3240

agcaagctga cagtggacaa gtcccggtgg cagcagggca acgtgttctc ctgctccgtg     3300

atgcacgagg ccctgcacaa ccactatacc cagaagtccc tgtccctgag ccccggcaag     3360

tgaggatccg acacagtctc tgtgccttct agttgccagc catctgttgt ttgcccctcc     3420

cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag     3480

gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag     3540

gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct     3600

atggagtgcg atgtgaggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt     3660

ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg     3720

ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga     3780

accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag     3840

aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc     3900

ttgcgtgcct tgaattactt ccacctggct ccagtacgtg attcttgatc ccgagctgga     3960

gccaggggcg ggccttgcgc tttaggagcc ccttcgcctc gtgcttgagt tgaggcctgg     4020

cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct     4080

ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg     4140

caagatagtc ttgtaaatgc gggccaggat ctgcacactg gtatttcggt ttttggggcc     4200

gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga     4260

gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct     4320

ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca     4380

gttgcgtgag cggaaagatg gccgcttccc ggccctgctc cagggggctc aaaatggagg     4440

acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaagg ggcctttccg     4500

tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat     4560

tagttctgga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg     4620

gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa     4680

ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca     4740

gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa cacgtggtcg cggccgcacc     4800

atgggctggt cctgcatcat cctgtttctg gtggctaccg ccaccggcgt gcactcccag     4860

attgtgctgt ctcagtcccc cgccatcctg tctgctagcc ctggcgagaa agtgacaatg     4920

acctgccggg cctcctcctc cgtgtcctac atccactggt tccagcagaa gcccggctcc     4980

agccccaagc cttggatcta cgccacctcc aacctggcct ctggcgtgcc agtgcggttt     5040

tccggctctg gctctggcac ctcctactcc ctgaccatct ctcgggtgga agccgaggat     5100

gccgccacct actactgcca gcagtggacc agcaaccccc ccacatttgg cggaggcacc     5160

aagctggaaa tcaagcggac cgtggccgct ccctccgtgt tcatcttccc accttccgac     5220

gagcagctga agtccggcac cgcttctgtc gtgtgcctgc tgaacaactt ctacccccgc     5280

gaggccaagg tgcagtggaa ggtggacaac gccctgcagt ccggcaactc ccaggaatcc     5340

gtgaccgagc aggactccaa ggacagcacc tacagcctgt cctccaccct gaccctgtcc     5400

aaggccgact acgagaagca caaggtgtac gcctgcgaag tgacccacca gggcctgtct     5460

agccccgtga ccaagtcttt caaccggggc gagtgctgac tcgagagatc tggccggctg     5520

ggcccgtttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac     5580

cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga ctgtgccttc     5640

tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc     5700

cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg     5760

tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa     5820

tagcaggcat gctggggatg cggtgggctc tatggaggtc tgagtgattg tcttaagcat     5880

agagtcaatg aaaagactca acagccttct cagtgttccg gaaaagtgtc ctgaacaagg     5940

tgggatgatt tggaagatat ctgaagataa gcagctagca gtctgcctga aatatgctgg     6000

agtatttgcg gaaaatgcgg aagacgctga tagaaaagat gtatttaata ccaaatctgt     6060

tgggcttttc attaaagagg ccatgtctaa ccacccgaac caggtagtag aaggatgctg     6120

ttccaatatg gctgtcactt ttaatggact aactcctaat cagatgcatg tgatgatgta     6180

tggggtgtac cggcttaggg cctttggaca tgttttcaac gatgcgttgg ttttcttacc     6240

tccaaacggt tctgataatg actgacaaaa agcaagagca tgcatttggt aaccacatta     6300

agacatgtta tgctttctaa tcgataatgc atctaacaca gtagtgtgtt tcttttcctt     6360

atctggtcac attgaagtct acttgtacat tttcaaatgg aatggtattt ttttccctta     6420

aatcatttgt gagaaatttt aatgtgttag aaataaatgt tttaagaata gcaattttgc     6480

aaataatgta tttataaata ttatatttat gtgataaaga ccaaattata gacattaaaa     6540

tctgtgatgt atctttgcct attgatttta aatgtttaat gtatcttttt agatttcaaa     6600

tatatgcaaa tgaggacgtc gctgttgaca ttgattattg actagttatt aatagtaatc     6660

aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt     6720

aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta     6780

tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg     6840

gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga     6900

cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt     6960

tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg     7020

gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc     7080

cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg     7140

taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat     7200

aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaatagtgcg     7260

atcgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg     7320

cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag     7380

ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc     7440

cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac     7500

gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag     7560

tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc     7620

ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac     7680

ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc     7740

gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac     7800

tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc     7860

tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa     7920

cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtag     7980

acacagtctc tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt     8040

ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat     8100

cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg     8160

gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggacttgc     8220

gtagtgagtc gaataagggc gacacaaaat ttattctaaa tgcataataa atactgataa     8280

catcttatag tttgtattat attttgtatt atcgttgaca tgtataattt tgatatcaaa     8340

aactgatttt ccctttatta ttttcgagat ttattttctt aattctcttt aacaaactag     8400

aaatattgta tatacaaaaa atcataaata atagatgaat agtttaatta taggtgttca     8460

tcaatcgaaa aagcaacgta tcttatttaa agtgcgttgc ttttttctca tttataaggt     8520

taaataattc tcatatatca agcaaagtga caggcgccct taaatattct gacaaatgct     8580

ctttccctaa actcccccca taaaaaaacc cgccgaagcg ggtttttacg ttatttgcgg     8640

attaacgatt actcgttatc agaaccgccc agggggcccg agcttaagac tggccgtcgt     8700

tttacaacac agaaagagtt tgtagaaacg caaaaaggcc atccgtcagg ggccttctgc     8760

ttagtttgat gcctggcagt tccctactct cgccttccgc ttcctcgctc actgactcgc     8820

tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt     8880

tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg     8940

ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg     9000

agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat     9060

accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta     9120

ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct     9180

gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc     9240

ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa     9300

gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg     9360

taggcggtgc tacagagttc ttgaagtggt gggctaacta cggctacact agaagaacag     9420

tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt     9480

gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta     9540

cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc     9600

agtggaacga cgcgcgcgta actcacgtta agggattttg gtcatgagct tgcgccgtcc     9660

cgtcaagtca gcgtaatgct ctgcttttac caatgcttaa tcagtgaggc acctatctca     9720

gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg     9780

atacgggagg gcttaccatc tggccccagc gctgcgatga taccgcgaga accacgctca     9840

ccggctccgg atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt     9900

cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt     9960

agttcgccag ttaatagttt gcgcaacgtt gttgccatcg ctacaggcat cgtggtgtca    10020

cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca    10080

tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga    10140

agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact    10200

gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga    10260

gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg    10320

ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc    10380

tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga    10440

tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat    10500

gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatatt cttccttttt    10560

caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt    10620

atttagaaaa ataaacaaat aggggtcagt gttacaacca attaaccaat tctgaacatt    10680

atcgcgagcc catttatacc tgaatatggc tcataacacc ccttgtttgc ctggcggcag    10740

tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga    10800

tggtagtgtg gggactcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa    10860

aggctcagtc gaaagactgg gcctttcgcc cgggctaatt atggggtgtc gcccttattc    10920

gactc                                                                10925


<210>  109
<211>  10925
<212>  DNA
<213>  artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  misc_feature
<222>  (1)..(10925)
<223>  COSMC-Rituximab-HDR-TI; Donor DNA plasmid with Rituximab

<400>  109
agtcggtgtg taatccatgg aggagtttct ataatgttgc agtttctacc taatggtgac       60

caaatgccag tgaaaggatt gtaagagtac ttgtcacata tactactcac ctcatttcaa      120

gaatgtggac ctgcttttaa acattaagag caaatcgtaa ttatataaga aataagcaaa      180

tgaaactatt agactgtttg aaaagtcttt ttctttacag gaaaaatgct ttcagaaagc      240

agttcatttt tgaaaggtgt gatgcttgga agcatcttct atgccttgat cactacgcta      300

ggccacatta ggattgggca cagaaacagg acacaccacc atgagcatca ccacctgcaa      360

gctcctaaca aagaagatat ctcgaaaatc tcagcggctg agcgcatgga gctcagtaag      420

agcttccggg tatactgtat agttcttgta aaacccaaag atgtgagtct ttgggctgca      480

gtgaaggaga cttggaccaa acactgtgac aaagcagagt tcttcagttc tgaaaatgtt      540

aaagtgtttg agtcaattaa cgtggacact gatgacatgt ggttgatgat gaggaaagct      600

tataaatatg cctttgataa atacaaagag cagtacaact ggttcttcct tgcacgcccc      660

agtacttttg ctgtgattga aaatctaaaa tattttttgt taaaaaagga tccatcgcag      720

cctttctatc taggacacac tgtaaaatct ggagacctta agcagcgtgt gaggctccgg      780

tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt      840

cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt      900

gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc      960

cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg     1020

ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac     1080

ctggctccag tacgtgattc ttgatcccga gctggagcca ggggcgggcc ttgcgcttta     1140

ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg     1200

cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa     1260

aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc     1320

caggatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg     1380

tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg     1440

gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc     1500

ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg     1560

cttcccggcc ctgctccagg gggctcaaaa tggaggacgc ggcgctcggg agagcgggcg     1620

ggtgagtcac ccacacaaag gaaaggggcc tttccgtcct cagccgtcgc ttcatgtgac     1680

tccacggagt accgggcgcc gtccaggcac ctcgattagt tctggagctt ttggagtacg     1740

tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg     1800

gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt     1860

gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca     1920

tttcaggtgt cgtgaagacg tcatgacacc atgggctggt cctgcatcat cctgtttctg     1980

gtggctaccg ccaccggcgt gcactctcag gtgcagctgc agcagcctgg cgccgagctc     2040

gtgaaacctg gcgcctccgt gaagatgtcc tgcaaggcct ccggctacac cttcaccagc     2100

tacaacatgc actgggtcaa gcagaccccc ggcagaggcc tggaatggat cggcgctatc     2160

taccccggca acggcgacac ctcctacaac cagaagttca agggcaaggc caccctgacc     2220

gccgacaagt cctcttccac cgcctacatg cagctgtcct ccctgacctc cgaggactcc     2280

gccgtgtact actgcgcccg gtctacctac tacggcggcg actggtactt caacgtgtgg     2340

ggcgctggca ccaccgtgac cgtgtctgct gcttctacca agggcccctc cgtgttccct     2400

ctggcccctt ccagcaagtc tacctctggc ggcacagccg ctctgggctg cctcgtgaag     2460

gactacttcc ccgagcccgt gacagtgtcc tggaactctg gcgctctgac cagcggagtg     2520

cacaccttcc ctgctgtgct gcagtcctcc ggcctgtact ccctgtccag cgtcgtgact     2580

gtgccctcca gctctctggg cacccagacc tacatctgca acgtgaacca caagccctcc     2640

aacaccaagg tggacaagaa ggtggaaccc aagtcctgcg acaagaccca cacctgtccc     2700

ccttgtcctg cccctgaact gctgggcgga cccagcgtgt tcctgttccc cccaaagccc     2760

aaggataccc tgatgatctc ccggaccccc gaagtgacct gcgtggtggt ggatgtgtcc     2820

cacgaggacc ctgaagtgaa gttcaattgg tacgtggacg gcgtggaagt gcacaacgcc     2880

aagaccaagc ctagagagga acagtacaac tccacctacc gggtggtgtc cgtgctgacc     2940

gtgctgcacc aggattggct gaacggcaaa gagtacaagt gcaaggtgtc caacaaggcc     3000

ctgcctgccc ccatcgaaaa gaccatctcc aaggccaagg gccagccccg ggaaccccag     3060

gtgtacacac tgccccctag cagggacgag ctgaccaaga accaggtgtc cctgacatgc     3120

ctcgtgaaag gcttctaccc ctccgatatc gccgtggaat gggagtccaa cggccagcct     3180

gagaacaact acaagaccac cccccctgtg ctggactccg acggctcatt cttcctgtac     3240

agcaagctga cagtggacaa gtcccggtgg cagcagggca acgtgttctc ctgctccgtg     3300

atgcacgagg ccctgcacaa ccactatacc cagaagtccc tgtccctgag ccccggcaag     3360

tgaggatccg acacagtctc tgtgccttct agttgccagc catctgttgt ttgcccctcc     3420

cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag     3480

gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag     3540

gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct     3600

atggagtgcg atgtgaggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt     3660

ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg     3720

ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga     3780

accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag     3840

aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc     3900

ttgcgtgcct tgaattactt ccacctggct ccagtacgtg attcttgatc ccgagctgga     3960

gccaggggcg ggccttgcgc tttaggagcc ccttcgcctc gtgcttgagt tgaggcctgg     4020

cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct     4080

ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg     4140

caagatagtc ttgtaaatgc gggccaggat ctgcacactg gtatttcggt ttttggggcc     4200

gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga     4260

gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct     4320

ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca     4380

gttgcgtgag cggaaagatg gccgcttccc ggccctgctc cagggggctc aaaatggagg     4440

acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaagg ggcctttccg     4500

tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat     4560

tagttctgga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg     4620

gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa     4680

ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca     4740

gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa cacgtggtcg cggccgcacc     4800

atgggctggt cctgcatcat cctgtttctg gtggctaccg ccaccggcgt gcactcccag     4860

attgtgctgt ctcagtcccc cgccatcctg tctgctagcc ctggcgagaa agtgacaatg     4920

acctgccggg cctcctcctc cgtgtcctac atccactggt tccagcagaa gcccggctcc     4980

agccccaagc cttggatcta cgccacctcc aacctggcct ctggcgtgcc agtgcggttt     5040

tccggctctg gctctggcac ctcctactcc ctgaccatct ctcgggtgga agccgaggat     5100

gccgccacct actactgcca gcagtggacc agcaaccccc ccacatttgg cggaggcacc     5160

aagctggaaa tcaagcggac cgtggccgct ccctccgtgt tcatcttccc accttccgac     5220

gagcagctga agtccggcac cgcttctgtc gtgtgcctgc tgaacaactt ctacccccgc     5280

gaggccaagg tgcagtggaa ggtggacaac gccctgcagt ccggcaactc ccaggaatcc     5340

gtgaccgagc aggactccaa ggacagcacc tacagcctgt cctccaccct gaccctgtcc     5400

aaggccgact acgagaagca caaggtgtac gcctgcgaag tgacccacca gggcctgtct     5460

agccccgtga ccaagtcttt caaccggggc gagtgctgac tcgagagatc tggccggctg     5520

ggcccgtttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac     5580

cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga ctgtgccttc     5640

tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc     5700

cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg     5760

tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa     5820

tagcaggcat gctggggatg cggtgggctc tatggaggtc tgagtgattg tcttaagcat     5880

agagtcaatg aaaagactca acagccttct cagtgttccg gaaaagtgtc ctgaacaagg     5940

tgggatgatt tggaagatat ctgaagataa gcagctagca gtctgcctga aatatgctgg     6000

agtatttgcg gaaaatgcgg aagacgctga tagaaaagat gtatttaata ccaaatctgt     6060

tgggcttttc attaaagagg ccatgtctaa ccacccgaac caggtagtag aaggatgctg     6120

ttccaatatg gctgtcactt ttaatggact aactcctaat cagatgcatg tgatgatgta     6180

tggggtgtac cggcttaggg cctttggaca tgttttcaac gatgcgttgg ttttcttacc     6240

tccaaacggt tctgataatg actgacaaaa agcaagagca tgcatttggt aaccacatta     6300

agacatgtta tgctttctaa tcgataatgc atctaacaca gtagtgtgtt tcttttcctt     6360

atctggtcac attgaagtct acttgtacat tttcaaatgg aatggtattt ttttccctta     6420

aatcatttgt gagaaatttt aatgtgttag aaataaatgt tttaagaata gcaattttgc     6480

aaataatgta tttataaata ttatatttat gtgataaaga ccaaattata gacattaaaa     6540

tctgtgatgt atctttgcct attgatttta aatgtttaat gtatcttttt agatttcaaa     6600

tatatgcaaa tgaggacgtc gctgttgaca ttgattattg actagttatt aatagtaatc     6660

aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt     6720

aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta     6780

tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg     6840

gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga     6900

cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt     6960

tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg     7020

gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc     7080

cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg     7140

taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat     7200

aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaatagtgcg     7260

atcgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg     7320

cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag     7380

ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc     7440

cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac     7500

gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag     7560

tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc     7620

ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac     7680

ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc     7740

gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac     7800

tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc     7860

tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa     7920

cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtag     7980

acacagtctc tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt     8040

ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat     8100

cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg     8160

gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggacttgc     8220

gtagtgagtc gaataagggc gacacaaaat ttattctaaa tgcataataa atactgataa     8280

catcttatag tttgtattat attttgtatt atcgttgaca tgtataattt tgatatcaaa     8340

aactgatttt ccctttatta ttttcgagat ttattttctt aattctcttt aacaaactag     8400

aaatattgta tatacaaaaa atcataaata atagatgaat agtttaatta taggtgttca     8460

tcaatcgaaa aagcaacgta tcttatttaa agtgcgttgc ttttttctca tttataaggt     8520

taaataattc tcatatatca agcaaagtga caggcgccct taaatattct gacaaatgct     8580

ctttccctaa actcccccca taaaaaaacc cgccgaagcg ggtttttacg ttatttgcgg     8640

attaacgatt actcgttatc agaaccgccc agggggcccg agcttaagac tggccgtcgt     8700

tttacaacac agaaagagtt tgtagaaacg caaaaaggcc atccgtcagg ggccttctgc     8760

ttagtttgat gcctggcagt tccctactct cgccttccgc ttcctcgctc actgactcgc     8820

tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt     8880

tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg     8940

ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg     9000

agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat     9060

accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta     9120

ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct     9180

gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc     9240

ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa     9300

gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg     9360

taggcggtgc tacagagttc ttgaagtggt gggctaacta cggctacact agaagaacag     9420

tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt     9480

gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta     9540

cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc     9600

agtggaacga cgcgcgcgta actcacgtta agggattttg gtcatgagct tgcgccgtcc     9660

cgtcaagtca gcgtaatgct ctgcttttac caatgcttaa tcagtgaggc acctatctca     9720

gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg     9780

atacgggagg gcttaccatc tggccccagc gctgcgatga taccgcgaga accacgctca     9840

ccggctccgg atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt     9900

cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt     9960

agttcgccag ttaatagttt gcgcaacgtt gttgccatcg ctacaggcat cgtggtgtca    10020

cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca    10080

tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga    10140

agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact    10200

gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga    10260

gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg    10320

ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc    10380

tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga    10440

tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat    10500

gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatatt cttccttttt    10560

caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt    10620

atttagaaaa ataaacaaat aggggtcagt gttacaacca attaaccaat tctgaacatt    10680

atcgcgagcc catttatacc tgaatatggc tcataacacc ccttgtttgc ctggcggcag    10740

tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga    10800

tggtagtgtg gggactcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa    10860

aggctcagtc gaaagactgg gcctttcgcc cgggctaatt atggggtgtc gcccttattc    10920

gactc                                                                10925


<210>  110
<211>  9944
<212>  DNA
<213>  artificial sequence

<220>
<223>  artificial sequence


<220>
<221>  misc_feature
<222>  (1)..(9944)
<223>  pJTIR4::2AGFP_Cas9

<400>  110
tcacgacacc tgaaatggaa gaaaaaaact ttgaaccact gtctgaggct tgagaatgaa       60

ccaagatcca aactcaaaaa gggcaaattc caaggagaat tacatcaagt gccaagctgg      120

cctaacttca gtctccaccc actcagtgtg gggaaactcc atcgcataaa acccctcccc      180

ccaacctaaa gacgacgtac tccaaaagct cgagaactaa tcgaggtgcc tggacggcgc      240

ccggtactcc gtggagtcac atgaagcgac ggctgaggac ggaaaggccc ttttcctttg      300

tgtgggtgac tcacccgccc gctctcccga gcgccgcgtc ctccattttg agctccctgc      360

agcaggaccg ggaagcggcc atctttccgc tcacgcaact ggtgccgacc gggccagcct      420

tgccgcccgg ggcggggcga tacacggcgg cgcgaggaca ggcaccagag caggccggcc      480

agcttgagac tacccccgtc cgattctcgg tggtcgcgct cgcaggcccc gcctcgccga      540

acatgtgcgc tgggacgcac gggccccgtc gccgcccgcg gccccaaaaa ccgaaatacc      600

agtgtgcaga tcttggcccg catttacaag actatcttgc cagaaaaaaa gcgtcgcagc      660

aggtcatcaa aaattttaaa tggctagaga cttatcgaaa gcagcgagac aggcgcgaag      720

gtgccaccag attcgcacgc ggcggcccca gcgcccaggc caggcctcaa ctcaagcacg      780

aggcgaaggg gctccttaag cgcaaggcct cgaactctcc cacccacttc caacccgaag      840

ctcgggatca agaatcacgt actgcagcca ggtggaagta attcaaggca cgcaagggcc      900

ataacccgta aagaggccag gcccgcggga accacacacg gcacttacct gtgttctggc      960

ggcaaacccg ttgcgaaaaa gaacgttcac ggcgactact gcacttatat acggttctcc     1020

cccaccctcg ggaaaaaggc ggagccagta cacggcatca ctttcccagt ttaccccgcg     1080

ccaccttctc taggcaccgg ttcaattgcc gacccctccc cccaacttct cggggactgt     1140

gggcgatgtg cgctctgccc actgacgggc accggagcca agccgaattc cagcacactg     1200

gcggccgtta ctagtggatc cgagctcggt accgatgaag aatctgctta gggttaggcg     1260

ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag     1320

ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt     1380

tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac     1440

gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg     1500

ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag     1560

tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat     1620

gaccttatgg gactttccta cttggcagta catctagtat tagtcatcgc tattaccatg     1680

gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt     1740

ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac     1800

tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg     1860

tgggaggtct atataagcag agctctccct atcagtgata gagatctccc tatcagtgat     1920

agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc     1980

tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct agaggatccc     2040

taccggtgat atcctcgagc ccatcagagc caccatgcat gtgagcaagg gcgaggagct     2100

gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt     2160

cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat     2220

ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg     2280

cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc     2340

catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa     2400

gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg     2460

catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag     2520

ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat     2580

ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc     2640

catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct     2700

gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc     2760

cgggatcact ctcggcatgg acgagctgta caagcctagg ggcagtggag agggcagagg     2820

aagtctgcta acatgcggtg acgtcgagga gaatcctggc ccacgatcga gaagcatgga     2880

caagaaatac tccatcggcc ttgatatcgg gaccaacagc gtgggttggg ccgtgatcac     2940

tgacgaatac aaagtgccat cgaaaaagtt taaggtgctg ggcaatactg acagacatag     3000

catcaagaaa aacctgattg gagccctcct gttcgactcc ggggaaaccg ccgaagccac     3060

tcgcctgaaa cggaccgcac gccggaggta cactcgccgg aagaatagaa tctgctacct     3120

tcaagagatc tttagcaacg agatggccaa agtggacgat tcgttcttcc accggctgga     3180

ggagtcattc ctggtcgaag aggacaaaaa gcatgagaga catccgatct tcggtaacat     3240

tgtggatgag gtcgcctacc acgagaagta ccccactatc taccatttgc gcaagaagtt     3300

ggtcgactcg actgataagg ccgacctcag actcatctac ctcgctttgg cacacatgat     3360

caagtttagg ggtcacttcc tgattgaagg ggatctgaac ccagacaact cggatgtgga     3420

taaactgttt atccagctgg tccagactta caatcagctc tttgaggaga acccgatcaa     3480

tgcctccggc gtggatgcta aggcaatcct gtccgcacgc ttgtcaaaga gccggcggct     3540

cgaaaatctg atcgcacagc ttccgggaga aaagaaaaac ggactgtttg gaaaccttat     3600

cgccctctcg ctgggactca ctcctaactt caaatccaat tttgatttgg ccgaggatgc     3660

caagctgcag ctgtcgaagg acacctacga cgatgacctc gacaatcttc tggcccagat     3720

cggggaccag tacgccgatc tgttcctcgc ggccaaaaac ctgtcagacg caatcttgct     3780

gagcgatatc ttgagggtca ataccgaaat cactaaggct ccattgtcag catcgatgat     3840

caagaggtac gatgaacacc atcaggacct gaccctgctc aaggcactgg tgcggcagca     3900

actgccggaa aagtacaagg agatcttctt cgatcaaagc aagaatgggt acgcagggta     3960

catcgatggt ggagcatcac aagaagagtt ctacaaattc atcaagccca tccttgaaaa     4020

gatggacggg acggaagaac tgctggtgaa gctgaatcgg gaagatctgc tgcggaagca     4080

gcgcaccttc gacaatggtt ccatcccaca ccaaatccat ctcggcgaac tgcacgcgat     4140

ccttcgccgg caggaagatt tctacccgtt cttgaaggat aacagagaaa agatcgagaa     4200

aatcctgacc tttagaatcc cgtactacgt gggcccgttg gctcgcggaa actcaagatt     4260

cgcctggatg actagaaaat ccgaagaaac gattaccccg tggaactttg aagaggtcgt     4320

cgacaaggga gcctcggcac agtcgttcat cgaacggatg accaatttcg acaagaacct     4380

ccccaacgaa aaggtgctcc cgaaacactc gctgctgtat gagtacttca cggtctacaa     4440

cgagctgacc aaggtgaagt acgtgaccga aggaatgcgg aagcccgctt ttctgtccgg     4500

tgaacagaaa aaggccatcg tggacctcct cttcaagact aacagaaagg tgaccgtgaa     4560

acagcttaaa gaggactact tcaagaagat cgagtgtttt gactccgtgg aaatctcggg     4620

agtggaggat aggtttaacg cttcgctggg gacctaccat gaccttctca agatcatcaa     4680

ggataaggat ttcctggaca acgaagaaaa cgaggacatc cttgaggaca tcgtgttgac     4740

cttgaccctg tttgaggatc gggaaatgat cgaggagaga ctgaaaactt acgcgcatct     4800

tttcgacgac aaagtgatga agcagctgaa aagaagaaga tacactggct ggggacggct     4860

gtcgaggaaa ctgattaacg gaattaggga taaacaaagc ggaaagacta tcctcgattt     4920

tctcaagtcg gacggcttcg ccaatcggaa ctttatgcag ctcatccacg acgatagcct     4980

gaccttcaag gaagatatcc aaaaagccca agtgtccggc cagggagata gcttgcacga     5040

gcacattgct aacctggccg gttcaccagc cattaagaag ggaatcctcc aaaccgtgaa     5100

ggtggtcgac gaactcgtca aggtgatggg ccgccataag ccagagaaca tcgtgatcga     5160

gatggctcgc gaaaatcaga ctactcagaa gggtcaaaag aactcccgcg agcgcatgaa     5220

gcgcatcgaa gaaggaatta aggagctggg ctcacaaatc ctcaaagaac atcctgtcga     5280

gaacacccaa cttcagaacg agaaactgta cctctactat ctccaaaatg gccgggacat     5340

gtacgtcgat caggaattgg atatcaaccg cctctcagac tacgacgtgg atcatatcgt     5400

gccgcagtcg tttctcaaag atgatagcat cgacaacaag gtcctgacga ggtcggataa     5460

gaaccgcggg aaatccgaca atgtgccttc ggaagaggtg gtgaaaaaga tgaagaacta     5520

ctggaggcaa ttgcttaatg ccaaactgat cacccagcgc aaattcgaca accttactaa     5580

ggccgagcgc ggaggactgt ccgaactcga taaggcgggg ttcatcaaaa gacaattggt     5640

cgaaactcgg caaattacca agcatgtcgc tcagatcctc gactcgcgca tgaacactaa     5700

gtatgatgag aacgacaagc tcattcgcga agtgaaagtg attaccctca aatcaaagct     5760

ggtgtcggac ttcagaaaag actttcaatt ctacaaggtg cgggagatta acaactacca     5820

ccacgcgcac gacgcctacc tcaatgcagt ggtgggaacc gccctcatca agaagtatcc     5880

aaagctcgaa agcgagttcg tgtacgggga ctataaagtg tacgatgtgc ggaaaatgat     5940

cgcaaagagc gagcaagaga tcggcaaagc aacggccaaa tacttcttct acagcaacat     6000

tatgaacttt ttcaagaccg agattaccct ggcgaacgga gaaatccgga agcggccttt     6060

gatcgaaacg aatggggaaa ctggagaaat cgtgtgggac aagggcagag actttgcgac     6120

cgtgagaaag gtgctgtcca tgccacaagt caacatcgtg aagaaaactg aagtgcagac     6180

tggaggattt tccaaggagt caatccttcc gaagcgcaac agcgacaagc tgatcgccag     6240

gaagaaggat tgggacccca agaagtacgg tggttttgat tcacctactg tcgcttacag     6300

cgtgctggtg gtggccaagg tggaaaaggg gaagtcaaag aaattgaagt cggtgaagga     6360

attgctgggg attactatca tggagaggag cagcttcgaa aagaatccca tcgacttctt     6420

ggaggccaag ggatacaaag aggtgaagaa agacctgatc attaagctgc cgaagtactc     6480

cttgttcgaa ctggaaaacg gcagaaagcg gatgctggcc tccgccggag agctgcagaa     6540

gggtaacgag ctggctctgc ccagcaaata cgtgaatttc ctctacctgg cctcccatta     6600

cgagaagctc aagggaagcc cggaggataa tgagcaaaaa caactgttcg tcgagcagca     6660

caagcactac ctcgacgaga tcatcgaaca aatctccgag ttctcgaagc gggtgattct     6720

ggccgacgca aaccttgata aagtcctcag cgcctacaac aagcaccgcg acaaaccgat     6780

cagagaacaa gctgagaaca tcatccacct gttcacgctg actaacttgg gagcgcctgc     6840

cgccttcaag tacttcgaca ccactatcga tcggaaacgg tacacttcca ctaaggaagt     6900

gctggacgca accctgatcc atcagtccat caccggactt tatgagactc ggatcgatct     6960

cagccagctt ggcggtgatt caagagctga cccgaagaag aagcgcaagg tctagagctt     7020

gattgataac gggaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg     7080

ttagtaatga gtttaaacgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag     7140

gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt     7200

gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc     7260

cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt     7320

gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc agatctgcgc     7380

agctggggaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg     7440

ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa     7500

tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac     7560

ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt     7620

gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga     7680

gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca     7740

ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg     7800

ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt     7860

cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc     7920

ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct     7980

tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc     8040

gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta     8100

tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca     8160

gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag     8220

tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag     8280

ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt     8340

agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa     8400

gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg     8460

attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga     8520

agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta     8580

atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc     8640

cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg     8700

ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga     8760

agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt     8820

tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt     8880

gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc     8940

caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc     9000

ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca     9060

gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag     9120

tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg     9180

tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa     9240

cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa     9300

cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga     9360

gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga     9420

atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg     9480

agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt     9540

ccccgaaaag tgcatctgca gaattcggct tcccggtcaa catccagtac acctccggca     9600

ccaccggctt ccccaagggc gccacgctct cccaccacaa catcctcaac aacggctact     9660

gggtgggccg cacggtcggc tacacggagc aggaccgggt gtgtctgccg gtgcccttct     9720

accactgctt cggcatggtc atgggcaact tgggcgccac ctcccacgcc gcctgcatcg     9780

tcatcccggc cccgtccttc gagccggcgg cgacgctgga ggcggtgcag cgggagaggt     9840

gcacgtccct gtacggcgtc cccacgaagc cgaattccac cacactggac tagatgcatg     9900

ctcgagcggc cgccagtgtg atggatatct gcagaattcg gctt                      9944


<210>  111
<211>  9970
<212>  DNA
<213>  artificial sequence

<220>
<223>  artificial sequence


<220>
<221>  misc_feature
<222>  (1)..(9970)
<223>  pcDNA4/TO::2AGFP_Cas9

<400>  111
gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt      240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      420

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt      480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggaacc      720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc      840

tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga      900

gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga      960

ctctagcgtt taaacttaag cttggtaccg ccaccatgca tgtgagcaag ggcgaggagc     1020

tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt     1080

tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca     1140

tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg     1200

gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg     1260

ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca     1320

agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg     1380

gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca     1440

gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga     1500

tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc     1560

ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccaagc     1620

tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg     1680

ccgggatcac tctcggcatg gacgagctgt acaagcctag gggcagtgga gagggcagag     1740

gaagtctgct aacatgcggt gacgtcgagg agaatcctgg cccacgatcg agaagcatgg     1800

acaagaaata ctccatcggc cttgatatcg ggaccaacag cgtgggttgg gccgtgatca     1860

ctgacgaata caaagtgcca tcgaaaaagt ttaaggtgct gggcaatact gacagacata     1920

gcatcaagaa aaacctgatt ggagccctcc tgttcgactc cggggaaacc gccgaagcca     1980

ctcgcctgaa acggaccgca cgccggaggt acactcgccg gaagaataga atctgctacc     2040

ttcaagagat ctttagcaac gagatggcca aagtggacga ttcgttcttc caccggctgg     2100

aggagtcatt cctggtcgaa gaggacaaaa agcatgagag acatccgatc ttcggtaaca     2160

ttgtggatga ggtcgcctac cacgagaagt accccactat ctaccatttg cgcaagaagt     2220

tggtcgactc gactgataag gccgacctca gactcatcta cctcgctttg gcacacatga     2280

tcaagtttag gggtcacttc ctgattgaag gggatctgaa cccagacaac tcggatgtgg     2340

ataaactgtt tatccagctg gtccagactt acaatcagct ctttgaggag aacccgatca     2400

atgcctccgg cgtggatgct aaggcaatcc tgtccgcacg cttgtcaaag agccggcggc     2460

tcgaaaatct gatcgcacag cttccgggag aaaagaaaaa cggactgttt ggaaacctta     2520

tcgccctctc gctgggactc actcctaact tcaaatccaa ttttgatttg gccgaggatg     2580

ccaagctgca gctgtcgaag gacacctacg acgatgacct cgacaatctt ctggcccaga     2640

tcggggacca gtacgccgat ctgttcctcg cggccaaaaa cctgtcagac gcaatcttgc     2700

tgagcgatat cttgagggtc aataccgaaa tcactaaggc tccattgtca gcatcgatga     2760

tcaagaggta cgatgaacac catcaggacc tgaccctgct caaggcactg gtgcggcagc     2820

aactgccgga aaagtacaag gagatcttct tcgatcaaag caagaatggg tacgcagggt     2880

acatcgatgg tggagcatca caagaagagt tctacaaatt catcaagccc atccttgaaa     2940

agatggacgg gacggaagaa ctgctggtga agctgaatcg ggaagatctg ctgcggaagc     3000

agcgcacctt cgacaatggt tccatcccac accaaatcca tctcggcgaa ctgcacgcga     3060

tccttcgccg gcaggaagat ttctacccgt tcttgaagga taacagagaa aagatcgaga     3120

aaatcctgac ctttagaatc ccgtactacg tgggcccgtt ggctcgcgga aactcaagat     3180

tcgcctggat gactagaaaa tccgaagaaa cgattacccc gtggaacttt gaagaggtcg     3240

tcgacaaggg agcctcggca cagtcgttca tcgaacggat gaccaatttc gacaagaacc     3300

tccccaacga aaaggtgctc ccgaaacact cgctgctgta tgagtacttc acggtctaca     3360

acgagctgac caaggtgaag tacgtgaccg aaggaatgcg gaagcccgct tttctgtccg     3420

gtgaacagaa aaaggccatc gtggacctcc tcttcaagac taacagaaag gtgaccgtga     3480

aacagcttaa agaggactac ttcaagaaga tcgagtgttt tgactccgtg gaaatctcgg     3540

gagtggagga taggtttaac gcttcgctgg ggacctacca tgaccttctc aagatcatca     3600

aggataagga tttcctggac aacgaagaaa acgaggacat ccttgaggac atcgtgttga     3660

ccttgaccct gtttgaggat cgggaaatga tcgaggagag actgaaaact tacgcgcatc     3720

ttttcgacga caaagtgatg aagcagctga aaagaagaag atacactggc tggggacggc     3780

tgtcgaggaa actgattaac ggaattaggg ataaacaaag cggaaagact atcctcgatt     3840

ttctcaagtc ggacggcttc gccaatcgga actttatgca gctcatccac gacgatagcc     3900

tgaccttcaa ggaagatatc caaaaagccc aagtgtccgg ccagggagat agcttgcacg     3960

agcacattgc taacctggcc ggttcaccag ccattaagaa gggaatcctc caaaccgtga     4020

aggtggtcga cgaactcgtc aaggtgatgg gccgccataa gccagagaac atcgtgatcg     4080

agatggctcg cgaaaatcag actactcaga agggtcaaaa gaactcccgc gagcgcatga     4140

agcgcatcga agaaggaatt aaggagctgg gctcacaaat cctcaaagaa catcctgtcg     4200

agaacaccca acttcagaac gagaaactgt acctctacta tctccaaaat ggccgggaca     4260

tgtacgtcga tcaggaattg gatatcaacc gcctctcaga ctacgacgtg gatcatatcg     4320

tgccgcagtc gtttctcaaa gatgatagca tcgacaacaa ggtcctgacg aggtcggata     4380

agaaccgcgg gaaatccgac aatgtgcctt cggaagaggt ggtgaaaaag atgaagaact     4440

actggaggca attgcttaat gccaaactga tcacccagcg caaattcgac aaccttacta     4500

aggccgagcg cggaggactg tccgaactcg ataaggcggg gttcatcaaa agacaattgg     4560

tcgaaactcg gcaaattacc aagcatgtcg ctcagatcct cgactcgcgc atgaacacta     4620

agtatgatga gaacgacaag ctcattcgcg aagtgaaagt gattaccctc aaatcaaagc     4680

tggtgtcgga cttcagaaaa gactttcaat tctacaaggt gcgggagatt aacaactacc     4740

accacgcgca cgacgcctac ctcaatgcag tggtgggaac cgccctcatc aagaagtatc     4800

caaagctcga aagcgagttc gtgtacgggg actataaagt gtacgatgtg cggaaaatga     4860

tcgcaaagag cgagcaagag atcggcaaag caacggccaa atacttcttc tacagcaaca     4920

ttatgaactt tttcaagacc gagattaccc tggcgaacgg agaaatccgg aagcggcctt     4980

tgatcgaaac gaatggggaa actggagaaa tcgtgtggga caagggcaga gactttgcga     5040

ccgtgagaaa ggtgctgtcc atgccacaag tcaacatcgt gaagaaaact gaagtgcaga     5100

ctggaggatt ttccaaggag tcaatccttc cgaagcgcaa cagcgacaag ctgatcgcca     5160

ggaagaagga ttgggacccc aagaagtacg gtggttttga ttcacctact gtcgcttaca     5220

gcgtgctggt ggtggccaag gtggaaaagg ggaagtcaaa gaaattgaag tcggtgaagg     5280

aattgctggg gattactatc atggagagga gcagcttcga aaagaatccc atcgacttct     5340

tggaggccaa gggatacaaa gaggtgaaga aagacctgat cattaagctg ccgaagtact     5400

ccttgttcga actggaaaac ggcagaaagc ggatgctggc ctccgccgga gagctgcaga     5460

agggtaacga gctggctctg cccagcaaat acgtgaattt cctctacctg gcctcccatt     5520

acgagaagct caagggaagc ccggaggata atgagcaaaa acaactgttc gtcgagcagc     5580

acaagcacta cctcgacgag atcatcgaac aaatctccga gttctcgaag cgggtgattc     5640

tggccgacgc aaaccttgat aaagtcctca gcgcctacaa caagcaccgc gacaaaccga     5700

tcagagaaca agctgagaac atcatccacc tgttcacgct gactaacttg ggagcgcctg     5760

ccgccttcaa gtacttcgac accactatcg atcggaaacg gtacacttcc actaaggaag     5820

tgctggacgc aaccctgatc catcagtcca tcaccggact ttatgagact cggatcgatc     5880

tcagccagct tggcggtgat tcaagagctg acccgaagaa gaagcgcaag gtctaggcgg     5940

ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt     6000

tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact     6060

cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat     6120

tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc     6180

aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc     6240

tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt     6300

acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc     6360

ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct     6420

ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat     6480

ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc     6540

acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc     6600

tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg     6660

atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa     6720

agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa     6780

ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca     6840

attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca     6900

gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg     6960

ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct     7020

tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga     7080

caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac     7140

catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt     7200

cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg     7260

tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga     7320

caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga     7380

ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca     7440

gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc     7500

cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag     7560

gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct     7620

catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata     7680

aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg     7740

tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag     7800

cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc     7860

acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta     7920

actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca     7980

gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc     8040

cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc     8100

tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat     8160

gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt     8220

ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg     8280

aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc     8340

tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt     8400

ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa     8460

gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta     8520

tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa     8580

caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa     8640

ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt     8700

cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt     8760

tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt     8820

ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag     8880

attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat     8940

ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc     9000

tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat     9060

aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc     9120

acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag     9180

aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag     9240

agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt     9300

ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg     9360

agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt     9420

tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc     9480

tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc     9540

attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa     9600

taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg     9660

aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc     9720

caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag     9780

gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt     9840

cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt     9900

tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc     9960

acctgacgtc                                                            9970


<210>  112
<211>  6610
<212>  DNA
<213>  artificial sequence

<220>
<223>  artificial sequence


<220>
<221>  misc_feature
<222>  (1)..(6610)
<223>  pcDNA6/TR _ZEO

<400>  112
gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt      240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      420

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt      480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca      840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc      900

gtttaaactt aagcttggta cccggggatc ctctagggcc tctgagctat tccagaagta      960

gtgaagaggc ttttttggag gcctaggctt ttgcaaaaag ctccggatcg atcctgagaa     1020

cttcagggtg agtttgggga cccttgattg ttctttcttt ttcgctattg taaaattcat     1080

gttatatgga gggggcaaag ttttcagggt gttgtttaga atgggaagat gtcccttgta     1140

tcaccatgga ccctcatgat aattttgttt ctttcacttt ctactctgtt gacaaccatt     1200

gtctcctctt attttctttt cattttctgt aactttttcg ttaaacttta gcttgcattt     1260

gtaacgaatt tttaaattca cttttgttta tttgtcagat tgtaagtact ttctctaatc     1320

actttttttt caaggcaatc agggtatatt atattgtact tcagcacagt tttagagaac     1380

aattgttata attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg     1440

aaatattctt attggtagaa acaactacat cctggtcatc atcctgcctt tctctttatg     1500

gttacaatga tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc     1560

ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt     1620

tattgtgctg tctcatcatt ttggcaaaga attgtaatac gactcactat agggcgaatt     1680

gatatgtcta gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag     1740

gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct     1800

acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg     1860

ttagataggc accatactca cttttgccct ttagaagggg aaagctggca agatttttta     1920

cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta     1980

catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt     2040

ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat     2100

tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa     2160

acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac     2220

caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa     2280

caacttaaat gtgaaagtgg gtccgcgtac agcggatccc gggaattcag atcttattaa     2340

agcagaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt     2400

tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg     2460

tatcttatca tgtctggtcg aatccatcac actggcggcc gctcgagtct agagggcccg     2520

cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt     2580

catcatcacc atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctagt     2640

tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact     2700

cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat     2760

tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc     2820

aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc     2880

tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt     2940

acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc     3000

ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct     3060

ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat     3120

ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc     3180

acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc     3240

tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg     3300

atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa     3360

agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa     3420

ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca     3480

attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca     3540

gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg     3600

ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct     3660

tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga     3720

caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac     3780

catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt     3840

cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg     3900

tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga     3960

caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga     4020

ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca     4080

gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc     4140

cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag     4200

gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct     4260

catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata     4320

aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg     4380

tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag     4440

cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc     4500

acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta     4560

actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca     4620

gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc     4680

cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc     4740

tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat     4800

gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt     4860

ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg     4920

aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc     4980

tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt     5040

ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa     5100

gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta     5160

tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa     5220

caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa     5280

ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt     5340

cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt     5400

tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt     5460

ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag     5520

attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat     5580

ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc     5640

tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat     5700

aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc     5760

acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag     5820

aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag     5880

agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt     5940

ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg     6000

agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt     6060

tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc     6120

tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc     6180

attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa     6240

taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg     6300

aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc     6360

caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag     6420

gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt     6480

cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt     6540

tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc     6600

acctgacgtc                                                            6610


