                         SEQUENCE LISTING

<110>  DANA-FARBER CANCER INSTITUTE, INC.
 
<120>  Methods For The Identification, Assessment, Prevention, And 
       Treatment Of Neurological Disorders And Diseases Using FNDC5

<130>  DFS-127.25

<140>  PCT/US2014/058649
<141>  2014-10-01

<150>  61/885,177
<151>  2013-10-01

<160>  51    

<170>  PatentIn version 3.5

<210>  1
<211>  630
<212>  DNA
<213>  Mus musculus

<400>  1
atgcccccag ggccgtgcgc ctggccgccc cgcgccgcgc tccgcctgtg gctaggctgc       60

gtctgcttcg cgctggtgca ggcggacagc ccctcagccc ctgtgaacgt gaccgtccgg      120

cacctcaagg ccaactctgc cgtggtcagc tgggatgtcc tggaggatga agtggtcatt      180

ggctttgcca tctctcagca gaagaaggat gtgcggatgc tccggttcat tcaggaggtg      240

aacaccacca cccggtcctg cgctctctgg gacctggagg aggacacaga atatatcgtc      300

catgtgcagg ccatctccat ccagggacag agcccagcca gtgagcctgt gctcttcaag      360

accccacgcg aggctgaaaa gatggcctca aagaacaaag atgaggtgac catgaaggag      420

atggggagga accagcagct gcgaacgggg gaggtgctga tcattgttgt ggtcctcttc      480

atgtgggcag gtgttatagc tctcttctgc cgccagtatg atatcatcaa ggacaacgag      540

cccaataaca acaaggagaa aaccaagagc gcatcagaaa ccagcacacc ggagcatcag      600

ggtgggggtc tcctccgcag caagatatga                                       630


<210>  2
<211>  209
<212>  PRT
<213>  Mus musculus

<400>  2

Met Pro Pro Gly Pro Cys Ala Trp Pro Pro Arg Ala Ala Leu Arg Leu 
1               5                   10                  15      


Trp Leu Gly Cys Val Cys Phe Ala Leu Val Gln Ala Asp Ser Pro Ser 
            20                  25                  30          


Ala Pro Val Asn Val Thr Val Arg His Leu Lys Ala Asn Ser Ala Val 
        35                  40                  45              


Val Ser Trp Asp Val Leu Glu Asp Glu Val Val Ile Gly Phe Ala Ile 
    50                  55                  60                  


Ser Gln Gln Lys Lys Asp Val Arg Met Leu Arg Phe Ile Gln Glu Val 
65                  70                  75                  80  


Asn Thr Thr Thr Arg Ser Cys Ala Leu Trp Asp Leu Glu Glu Asp Thr 
                85                  90                  95      


Glu Tyr Ile Val His Val Gln Ala Ile Ser Ile Gln Gly Gln Ser Pro 
            100                 105                 110         


Ala Ser Glu Pro Val Leu Phe Lys Thr Pro Arg Glu Ala Glu Lys Met 
        115                 120                 125             


Ala Ser Lys Asn Lys Asp Glu Val Thr Met Lys Glu Met Gly Arg Asn 
    130                 135                 140                 


Gln Gln Leu Arg Thr Gly Glu Val Leu Ile Ile Val Val Val Leu Phe 
145                 150                 155                 160 


Met Trp Ala Gly Val Ile Ala Leu Phe Cys Arg Gln Tyr Asp Ile Ile 
                165                 170                 175     


Lys Asp Asn Glu Pro Asn Asn Asn Lys Glu Lys Thr Lys Ser Ala Ser 
            180                 185                 190         


Glu Thr Ser Thr Pro Glu His Gln Gly Gly Gly Leu Leu Arg Ser Lys 
        195                 200                 205             


Ile 
    


<210>  3
<211>  462
<212>  DNA
<213>  Homo sapiens

<400>  3
atgctgcgct tcatccagga ggtgaacacc accacccgct catgtgccct ctgggacctg       60

gaggaggata cggagtacat agtccacgtg caggccatct ccattcaggg ccagagccca      120

gccagcgagc ctgtgctctt caagaccccg cgtgaggctg agaagatggc ctccaagaac      180

aaagatgagg taaccatgaa agagatgggg aggaaccaac agctgcggac aggcgaggtg      240

ctgatcatcg tcgtggtcct gttcatgtgg gcaggtgtca ttgccctctt ctgccgccag      300

tatgacatca tcaaggacaa tgaacccaat aacaacaagg aaaaaaccaa gagtgcatca      360

gaaaccagca caccagagca ccagggcggg gggcttctcc gcagcaaggt gagggcaaga      420

cctgggcctg ggtgggccac cctgtgcctc atgctctggt aa                         462


<210>  4
<211>  153
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Leu Arg Phe Ile Gln Glu Val Asn Thr Thr Thr Arg Ser Cys Ala 
1               5                   10                  15      


Leu Trp Asp Leu Glu Glu Asp Thr Glu Tyr Ile Val His Val Gln Ala 
            20                  25                  30          


Ile Ser Ile Gln Gly Gln Ser Pro Ala Ser Glu Pro Val Leu Phe Lys 
        35                  40                  45              


Thr Pro Arg Glu Ala Glu Lys Met Ala Ser Lys Asn Lys Asp Glu Val 
    50                  55                  60                  


Thr Met Lys Glu Met Gly Arg Asn Gln Gln Leu Arg Thr Gly Glu Val 
65                  70                  75                  80  


Leu Ile Ile Val Val Val Leu Phe Met Trp Ala Gly Val Ile Ala Leu 
                85                  90                  95      


Phe Cys Arg Gln Tyr Asp Ile Ile Lys Asp Asn Glu Pro Asn Asn Asn 
            100                 105                 110         


Lys Glu Lys Thr Lys Ser Ala Ser Glu Thr Ser Thr Pro Glu His Gln 
        115                 120                 125             


Gly Gly Gly Leu Leu Arg Ser Lys Val Arg Ala Arg Pro Gly Pro Gly 
    130                 135                 140                 


Trp Ala Thr Leu Cys Leu Met Leu Trp 
145                 150             


<210>  5
<211>  414
<212>  DNA
<213>  Homo sapiens

<400>  5
atgctgcgct tcatccagga ggtgaacacc accacccgct catgtgccct ctgggacctg       60

gaggaggata cggagtacat agtccacgtg caggccatct ccattcaggg ccagagccca      120

gccagcgagc ctgtgctctt caagaccccg cgtgaggctg agaagatggc ctccaagaac      180

aaagatgagg taaccatgaa agagatgggg aggaaccaac agctgcggac aggcgaggtg      240

ctgatcatcg tcgtggtcct gttcatgtgg gcaggtgtca ttgccctctt ctgccgccag      300

tatgacatca tcaaggacaa tgaacccaat aacaacaagg aaaaaaccaa gagtgcatca      360

gaaaccagca caccagagca ccagggcggg gggcttctcc gcagcaagat atga            414


<210>  6
<211>  137
<212>  PRT
<213>  Homo sapiens

<400>  6

Met Leu Arg Phe Ile Gln Glu Val Asn Thr Thr Thr Arg Ser Cys Ala 
1               5                   10                  15      


Leu Trp Asp Leu Glu Glu Asp Thr Glu Tyr Ile Val His Val Gln Ala 
            20                  25                  30          


Ile Ser Ile Gln Gly Gln Ser Pro Ala Ser Glu Pro Val Leu Phe Lys 
        35                  40                  45              


Thr Pro Arg Glu Ala Glu Lys Met Ala Ser Lys Asn Lys Asp Glu Val 
    50                  55                  60                  


Thr Met Lys Glu Met Gly Arg Asn Gln Gln Leu Arg Thr Gly Glu Val 
65                  70                  75                  80  


Leu Ile Ile Val Val Val Leu Phe Met Trp Ala Gly Val Ile Ala Leu 
                85                  90                  95      


Phe Cys Arg Gln Tyr Asp Ile Ile Lys Asp Asn Glu Pro Asn Asn Asn 
            100                 105                 110         


Lys Glu Lys Thr Lys Ser Ala Ser Glu Thr Ser Thr Pro Glu His Gln 
        115                 120                 125             


Gly Gly Gly Leu Leu Arg Ser Lys Ile 
    130                 135         


<210>  7
<211>  321
<212>  DNA
<213>  Homo sapiens

<400>  7
atgctgcgct tcatccagga ggtgaacacc accacccgct catgtgccct ctgggacctg       60

gaggaggata cggagtacat agtccacgtg caggccatct ccattcaggg ccagagccca      120

gccagcgagc ctgtgctctt caagaccccg cgtgaggctg agaagatggc ctccaagaac      180

aaagatgagg taaccatgaa agagatgggg aggaaccaac agctgcggac aggcgaggtg      240

ctgatcatcg tcgtggtcct gttcatgtgg gcaggtgtca ttgccctctt ctgccgccag      300

tatgacatca ttgaagcgtg a                                                321


<210>  8
<211>  106
<212>  PRT
<213>  Homo sapiens

<400>  8

Met Leu Arg Phe Ile Gln Glu Val Asn Thr Thr Thr Arg Ser Cys Ala 
1               5                   10                  15      


Leu Trp Asp Leu Glu Glu Asp Thr Glu Tyr Ile Val His Val Gln Ala 
            20                  25                  30          


Ile Ser Ile Gln Gly Gln Ser Pro Ala Ser Glu Pro Val Leu Phe Lys 
        35                  40                  45              


Thr Pro Arg Glu Ala Glu Lys Met Ala Ser Lys Asn Lys Asp Glu Val 
    50                  55                  60                  


Thr Met Lys Glu Met Gly Arg Asn Gln Gln Leu Arg Thr Gly Glu Val 
65                  70                  75                  80  


Leu Ile Ile Val Val Val Leu Phe Met Trp Ala Gly Val Ile Ala Leu 
                85                  90                  95      


Phe Cys Arg Gln Tyr Asp Ile Ile Glu Ala 
            100                 105     


<210>  9
<211>  1203
<212>  DNA
<213>  Gallus gallus

<400>  9
atggagaaga acagggacgg ccgcggcccc cctggtgtcc atctggggat ggagaaggaa       60

gatgatttag agcccggtga cacgccgggg ctgcgcgaag ccctggtggc gagatgtcac      120

cgctgccgcg cacccgccgg gggtctcacc gggacgggcc ccgtttgctc cttccggcga      180

tggggagcgg tccgggccga gggctcccgg tcccgcctgg gggaaactga ggcagacggc      240

ggggccgggc ggggcggggg ccgagccgcc cccgggccgg gggagggacc ggagcggggc      300

tgcccagcgc tgcagcgggc ggagccgggg ctcggcgggg ccgcctcccg gccgagccga      360

gccgaaccga gccgcgctgc cgagggccgc cgagcccgca gccgcccccg gccgaaccgg      420

gcggccccgc cggttccggg ccccggagct ctccgcggtg ctgaacggcg ccgccgcgcc      480

cgcgggacgc cggccccgga gcggctcggc cccggcgcgg cgcggcgggc cgcgggggga      540

tggagccctt cctgggctgc accggcgccg cgctcctgct ctgctttcag ctacgccggt      600

ctgcggccgg tggaggcaga cagcccttcg gctccggtca atgtcacagt caaacacctg      660

aaggccaact cagctgtagt gacttgggac gttctggagg atgaagttgt cattggattt      720

gccatttccc agcagaagaa ggacgtgcgg atgctgcgct tcatccagga ggtgaacacc      780

accacccgct cctgtgccct ctgggaccta gaggaggaca ctgagtacat tgtgcatgtc      840

caggccatca gcatccaagg ccagagccct gccagtgagc cagtcctctt caagaccccc      900

agggaagctg agaaactggc ttctaaaaat aaagatgagg tgacaatgaa ggagatggcg      960

aagaaaaacc aacagctgcg cgcaggggaa atactcatca ttgtggtggt gttgtttatg     1020

tgggcagggg tgatcgccct gttctgcagg cagtacgaca tcatcaaaga caacgagccg     1080

aacaacagca aggagaaagc caagagcgcc tcagagaaca gcacccccga gcaccagggt     1140

ggggggctgc tccgcagcaa gttcccaaaa aacaaaccct cagtgaacat cattgaggca     1200

taa                                                                   1203


<210>  10
<211>  400
<212>  PRT
<213>  Gallus gallus

<400>  10

Met Glu Lys Asn Arg Asp Gly Arg Gly Pro Pro Gly Val His Leu Gly 
1               5                   10                  15      


Met Glu Lys Glu Asp Asp Leu Glu Pro Gly Asp Thr Pro Gly Leu Arg 
            20                  25                  30          


Glu Ala Leu Val Ala Arg Cys His Arg Cys Arg Ala Pro Ala Gly Gly 
        35                  40                  45              


Leu Thr Gly Thr Gly Pro Val Cys Ser Phe Arg Arg Trp Gly Ala Val 
    50                  55                  60                  


Arg Ala Glu Gly Ser Arg Ser Arg Leu Gly Glu Thr Glu Ala Asp Gly 
65                  70                  75                  80  


Gly Ala Gly Arg Gly Gly Gly Arg Ala Ala Pro Gly Pro Gly Glu Gly 
                85                  90                  95      


Pro Glu Arg Gly Cys Pro Ala Leu Gln Arg Ala Glu Pro Gly Leu Gly 
            100                 105                 110         


Gly Ala Ala Ser Arg Pro Ser Arg Ala Glu Pro Ser Arg Ala Ala Glu 
        115                 120                 125             


Gly Arg Arg Ala Arg Ser Arg Pro Arg Pro Asn Arg Ala Ala Pro Pro 
    130                 135                 140                 


Val Pro Gly Pro Gly Ala Leu Arg Gly Ala Glu Arg Arg Arg Arg Ala 
145                 150                 155                 160 


Arg Gly Thr Pro Ala Pro Glu Arg Leu Gly Pro Gly Ala Ala Arg Arg 
                165                 170                 175     


Ala Ala Gly Gly Trp Ser Pro Ser Trp Ala Ala Pro Ala Pro Arg Ser 
            180                 185                 190         


Cys Ser Ala Phe Ser Tyr Ala Gly Leu Arg Pro Val Glu Ala Asp Ser 
        195                 200                 205             


Pro Ser Ala Pro Val Asn Val Thr Val Lys His Leu Lys Ala Asn Ser 
    210                 215                 220                 


Ala Val Val Thr Trp Asp Val Leu Glu Asp Glu Val Val Ile Gly Phe 
225                 230                 235                 240 


Ala Ile Ser Gln Gln Lys Lys Asp Val Arg Met Leu Arg Phe Ile Gln 
                245                 250                 255     


Glu Val Asn Thr Thr Thr Arg Ser Cys Ala Leu Trp Asp Leu Glu Glu 
            260                 265                 270         


Asp Thr Glu Tyr Ile Val His Val Gln Ala Ile Ser Ile Gln Gly Gln 
        275                 280                 285             


Ser Pro Ala Ser Glu Pro Val Leu Phe Lys Thr Pro Arg Glu Ala Glu 
    290                 295                 300                 


Lys Leu Ala Ser Lys Asn Lys Asp Glu Val Thr Met Lys Glu Met Ala 
305                 310                 315                 320 


Lys Lys Asn Gln Gln Leu Arg Ala Gly Glu Ile Leu Ile Ile Val Val 
                325                 330                 335     


Val Leu Phe Met Trp Ala Gly Val Ile Ala Leu Phe Cys Arg Gln Tyr 
            340                 345                 350         


Asp Ile Ile Lys Asp Asn Glu Pro Asn Asn Ser Lys Glu Lys Ala Lys 
        355                 360                 365             


Ser Ala Ser Glu Asn Ser Thr Pro Glu His Gln Gly Gly Gly Leu Leu 
    370                 375                 380                 


Arg Ser Lys Phe Pro Lys Asn Lys Pro Ser Val Asn Ile Ile Glu Ala 
385                 390                 395                 400 


<210>  11
<211>  555
<212>  DNA
<213>  Danio rerio

<400>  11
atgagttctt acagtttggc agctccagtg aatgtgtcca tcagggatct gaagagcagc       60

tcagccgtgg tgacatggga cacgccagac ggagagccag tcatcggctt cgccatcaca      120

caacagaaga aagatgtccg catgctgcgc tttattcaag aagtgaacac caccacgcgg      180

agctgtgcat tgtgggatct ggaagctgat acggattaca ttgtgcacgt tcagtctatc      240

agcatcagcg gggcgagtcc tgttagtgaa gctgtgcact tcaagacccc gacagaagtt      300

gaaacacagg cctccaagaa caaagacgag gtgacgatgg aggaggtcgg gccgaacgct      360

cagctcaggg ccggagagtt catcattatt gtggtggtcc tcatcatgtg ggcaggtgtg      420

atcgcactat tctgccgtca gtatgacatc attaaagaca acgaaccaaa caataacaag      480

gataaagcca agaactcgtc tgaatgcagc actccagagc acacgtcagg tggcctgctg      540

cgcagtaagg tataa                                                       555


<210>  12
<211>  184
<212>  PRT
<213>  Danio rerio

<400>  12

Met Ser Ser Tyr Ser Leu Ala Ala Pro Val Asn Val Ser Ile Arg Asp 
1               5                   10                  15      


Leu Lys Ser Ser Ser Ala Val Val Thr Trp Asp Thr Pro Asp Gly Glu 
            20                  25                  30          


Pro Val Ile Gly Phe Ala Ile Thr Gln Gln Lys Lys Asp Val Arg Met 
        35                  40                  45              


Leu Arg Phe Ile Gln Glu Val Asn Thr Thr Thr Arg Ser Cys Ala Leu 
    50                  55                  60                  


Trp Asp Leu Glu Ala Asp Thr Asp Tyr Ile Val His Val Gln Ser Ile 
65                  70                  75                  80  


Ser Ile Ser Gly Ala Ser Pro Val Ser Glu Ala Val His Phe Lys Thr 
                85                  90                  95      


Pro Thr Glu Val Glu Thr Gln Ala Ser Lys Asn Lys Asp Glu Val Thr 
            100                 105                 110         


Met Glu Glu Val Gly Pro Asn Ala Gln Leu Arg Ala Gly Glu Phe Ile 
        115                 120                 125             


Ile Ile Val Val Val Leu Ile Met Trp Ala Gly Val Ile Ala Leu Phe 
    130                 135                 140                 


Cys Arg Gln Tyr Asp Ile Ile Lys Asp Asn Glu Pro Asn Asn Asn Lys 
145                 150                 155                 160 


Asp Lys Ala Lys Asn Ser Ser Glu Cys Ser Thr Pro Glu His Thr Ser 
                165                 170                 175     


Gly Gly Leu Leu Arg Ser Lys Val 
            180                 


<210>  13
<211>  336
<212>  DNA
<213>  Mus musculus

<400>  13
gacagcccct cagcccctgt gaacgtgacc gtccggcacc tcaaggccaa ctctgccgtg       60

gtcagctggg atgtcctgga ggatgaagtg gtcattggct ttgccatctc tcagcagaag      120

aaggatgtgc ggatgctccg gttcattcag gaggtgaaca ccaccacccg gtcctgcgct      180

ctctgggacc tggaggagga cacagaatat atcgtccatg tgcaggccat ctccatccag      240

ggacagagcc cagccagtga gcctgtgctc ttcaagaccc cacgcgaggc tgaaaagatg      300

gcctcaaaga acaaagatga ggtgaccatg aaggag                                336


<210>  14
<211>  112
<212>  PRT
<213>  Mus musculus

<400>  14

Asp Ser Pro Ser Ala Pro Val Asn Val Thr Val Arg His Leu Lys Ala 
1               5                   10                  15      


Asn Ser Ala Val Val Ser Trp Asp Val Leu Glu Asp Glu Val Val Ile 
            20                  25                  30          


Gly Phe Ala Ile Ser Gln Gln Lys Lys Asp Val Arg Met Leu Arg Phe 
        35                  40                  45              


Ile Gln Glu Val Asn Thr Thr Thr Arg Ser Cys Ala Leu Trp Asp Leu 
    50                  55                  60                  


Glu Glu Asp Thr Glu Tyr Ile Val His Val Gln Ala Ile Ser Ile Gln 
65                  70                  75                  80  


Gly Gln Ser Pro Ala Ser Glu Pro Val Leu Phe Lys Thr Pro Arg Glu 
                85                  90                  95      


Ala Glu Lys Met Ala Ser Lys Asn Lys Asp Glu Val Thr Met Lys Glu 
            100                 105                 110         


<210>  15
<211>  336
<212>  DNA
<213>  Homo sapiens

<400>  15
gacagtccct cagccccagt gaacgtcacc gtcaggcacc tcaaggccaa ctctgcagtg       60

gtgagctggg atgttctgga ggatgaggtt gtcatcggat ttgccatctc ccagcagaag      120

aaggatgtgc ggatgctgcg cttcatccag gaggtgaaca ccaccacccg ctcatgtgcc      180

ctctgggacc tggaggagga tacggagtac atagtccacg tgcaggccat ctccattcag      240

ggccagagcc cagccagcga gcctgtgctc ttcaagaccc cgcgtgaggc tgagaagatg      300

gcctccaaga acaaagatga ggtaaccatg aaagag                                336


<210>  16
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: Synthetic primer"

<400>  16
catgcagaac ccacgacagt a                                                 21


<210>  17
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: Synthetic primer"

<400>  17
cctcacgcag cttgttgtct a                                                 21


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: Synthetic primer"

<400>  18
atgaaggaga tggggaggaa                                                   20


<210>  19
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: Synthetic primer"

<400>  19
gcggcagaag agagctataa ca                                                22


<210>  20
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: Synthetic primer"

<400>  20
tgatgtgaat gacttggata cagaca                                            26


<210>  21
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: Synthetic primer"

<400>  21
gctcattgtt gtactggttg gatatg                                            26


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: Synthetic primer"

<400>  22
cactacggtg tggcatcctg                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: Synthetic primer"

<400>  23
acagctgtac tcgatgctcc                                                   20


<210>  24
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: Synthetic primer"

<400>  24
aaccgaatgt cgtccgaaga c                                                 21


<210>  25
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: Synthetic primer"

<400>  25
gtggctgagg gcatcaatg                                                    19


<210>  26
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: Synthetic primer"

<400>  26
atggattcgg tagaactttg cc                                                22


<210>  27
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: Synthetic primer"

<400>  27
cttcttcgta gtgcagggaa aa                                                22


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: Synthetic primer"

<400>  28
tggccctgcg gaggctaagt                                                   20


<210>  29
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: Synthetic primer"

<400>  29
agggtgcttc cgagccttcc t                                                 21


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: Synthetic primer"

<400>  30
tggatgctct tcagttcgtg                                                   20


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: Synthetic primer"

<400>  31
gtcttgggca tgtcagtgtg                                                   20


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: Synthetic primer"

<400>  32
ctgcatctac actcgcaagg                                                   20


<210>  33
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: Synthetic primer"

<400>  33
gccacaatgt cttcaagctc t                                                 21


<210>  34
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: Synthetic primer"

<400>  34
atgggctctc ctgtcaacac ac                                                22


<210>  35
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: Synthetic primer"

<400>  35
atggctgtca ccgtggggat aaag                                              24


<210>  36
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: Synthetic primer"

<400>  36
taccgttagc ccctatgcca tc                                                22


<210>  37
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: Synthetic primer"

<400>  37
tgatattgct gagcctcaac tg                                                22


<210>  38
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: Synthetic primer"

<400>  38
tatgagcacc tgaccacaga gtcc                                              24


<210>  39
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: Synthetic primer"

<400>  39
cgagtcgttt ggctgggata ac                                                22


<210>  40
<211>  344
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: IrisinFc polypeptide"

<400>  40

Asp Ser Pro Ser Ala Pro Val Asn Val Thr Val Arg His Leu Lys Ala 
1               5                   10                  15      


Asn Ser Ala Val Val Ser Trp Asp Val Leu Glu Asp Glu Val Val Ile 
            20                  25                  30          


Gly Phe Ala Ile Ser Gln Gln Lys Lys Asp Val Arg Met Leu Arg Phe 
        35                  40                  45              


Ile Gln Glu Val Asn Thr Thr Thr Arg Ser Cys Ala Leu Trp Asp Leu 
    50                  55                  60                  


Glu Glu Asp Thr Glu Tyr Ile Val His Val Gln Ala Ile Ser Ile Gln 
65                  70                  75                  80  


Gly Gln Ser Pro Ala Ser Glu Pro Val Leu Phe Lys Thr Pro Arg Glu 
                85                  90                  95      


Ala Glu Lys Met Ala Ser Lys Asn Lys Asp Glu Val Thr Met Lys Glu 
            100                 105                 110         


Gly Gly Gly Gly Ala Gly Gly Gly Gly Val Glu Cys Pro Pro Cys Pro 
        115                 120                 125             


Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
    130                 135                 140                 


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
145                 150                 155                 160 


Val Asp Val Ser His Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val 
                165                 170                 175     


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
            180                 185                 190         


Phe Asn Ser Thr Phe Arg Val Val Ser Val Leu Thr Val Val His Gln 
        195                 200                 205             


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly 
    210                 215                 220                 


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro 
225                 230                 235                 240 


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr 
                245                 250                 255     


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
            260                 265                 270         


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
        275                 280                 285             


Lys Thr Thr Pro Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
    290                 295                 300                 


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
305                 310                 315                 320 


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
                325                 330                 335     


Ser Leu Ser Leu Ser Pro Gly Lys 
            340                 


<210>  41
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: Control Fc 
       polypeptide"

<400>  41

Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser Val 
1               5                   10                  15      


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
            20                  25                  30          


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
        35                  40                  45              


Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
    50                  55                  60                  


Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val Ser 
65                  70                  75                  80  


Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
                85                  90                  95      


Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr Ile 
            100                 105                 110         


Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
        115                 120                 125             


Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
    130                 135                 140                 


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
145                 150                 155                 160 


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp Ser 
                165                 170                 175     


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
            180                 185                 190         


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
        195                 200                 205             


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    210                 215                 220             


<210>  42
<211>  752
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: GFP nucleic acid"

<400>  42
aagcttggga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc       60

gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat      120

gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc      180

tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac      240

cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc      300

accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc      360

gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc      420

ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag      480

cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg      540

cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc      600

gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat      660

cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg      720

tacaagtaac gcggatccac tagttctaga gc                                    752


<210>  43
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: shFndc5-1 target 
       sequence"

<400>  43
ccctctgtga acatcatcaa a                                                 21


<210>  44
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: shFndc5-1 shRNA 
       oligonucleotide"

<400>  44
ccggccctct gtgaacatca tcaaactcga gtttgatgat gttcacagag ggtttttg         58


<210>  45
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: shFndc5-1 shRNA 
       oligonucleotide"

<400>  45
aattcaaaaa ccctctgtga acatcatcaa actcgagttt gatgatgttc acagaggg         58


<210>  46
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: shFndc5-2 target 
       sequence"

<400>  46
gtgcggatgc tccggttcat t                                                 21


<210>  47
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: shFndc5-2 shRNA 
       oligonucleotide"

<400>  47
ccgggtgcgg atgctccggt tcattctcga gaatgaaccg gagcatccgc actttttg         58


<210>  48
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: shFndc5-2 shRNA 
       oligonucleotide"

<400>  48
aattcaaaaa gtgcggatgc tccggttcat tctcgagaat gaaccggagc atccgcac         58


<210>  49
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: shFndc5-3 target 
       sequence"

<400>  49
cgagcccaat aacaacaagg a                                                 21


<210>  50
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: shFndc5-3 shRNA 
       oligonucleotide"

<400>  50
ccgggtgcgg atgctccggt tcattctcga gaatgaaccg gagcatccgc actttttg         58


<210>  51
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  /note="Description of Artificial Sequence: shFndc5-3 shRNA 
       oligonucleotide"

<400>  51
aattcaaaaa cgagcccaat aacaacaagg actcgagtcc ttgttgttat tgggctcg         58


