                         SEQUENCE LISTING

<110>  F-star Biotechnology Limited
       F-star Biotechnologische Forschungs- Und Entwicklungsges 
       m.b.H
 
<120>  Cancer Biomarkers and Uses Thereof

<130>  TEK/CP7067424

<150>  GB 1317622.7
<151>  2013-10-04

<160>  19    

<170>  PatentIn version 3.3

<210>  1
<211>  681
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: Nucleotide sequence of antigen-binding Fc 
       fragment H561-4

<400>  1
acctgccccc cttgtcctgc ccccgagctg ctgggaggcc cttccgtgtt tctgttcccc     60

ccaaagccca aggacaccct gatgatctcc cggacccccg aagtgacctg cgtggtggtg    120

gacgtgtccc acgaggaccc tgaagtgaag ttcaattggt acgtggacgg cgtggaagtg    180

cacaacgcca agaccaagcc cagagaggaa cagtacaact ccacctaccg ggtggtgtcc    240

gtgctgaccg tgctgcacca ggactggctg aacggcaaag agtacaagtg caaggtgtcc    300

aacaaggccc tgcctgcccc catcgaaaag accatctcca aggccaaggg ccagccccgc    360

gagccccagg tgtacaccct gccccctagc cgggacgagt tcttcaccta ctgggtgtcc    420

ctgacctgcc tggtcaaggg cttctacccc tccgatatcg ccgtggaatg ggagtccaac    480

ggccagcccg agaacaacta caagaccacc ccccctgtgc tggactccga cggctcattc    540

ttcctgtact ccaagctgac cgtggaccgg cggagatgga ccgccggcaa cgtgttctcc    600

tgcagcgtga tgcacgaggc cctgcacaac cactacaccc agaagtccct gtccctgagc    660

cccggcaagt gatgagaatt c                                              681


<210>  2
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: Nucleotide sequence of the antigen-binding Fc
       fragment H561-4 hinge region

<400>  2
acctgccccc cttgtcct                                                   18


<210>  3
<211>  330
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: Nucleotide sequence of the antigen-binding Fc
       fragment H561-4 CH2 domain

<400>  3
gcccccgagc tgctgggagg cccttccgtg tttctgttcc ccccaaagcc caaggacacc     60

ctgatgatct cccggacccc cgaagtgacc tgcgtggtgg tggacgtgtc ccacgaggac    120

cctgaagtga agttcaattg gtacgtggac ggcgtggaag tgcacaacgc caagaccaag    180

cccagagagg aacagtacaa ctccacctac cgggtggtgt ccgtgctgac cgtgctgcac    240

caggactggc tgaacggcaa agagtacaag tgcaaggtgt ccaacaaggc cctgcctgcc    300

cccatcgaaa agaccatctc caaggccaag                                     330


<210>  4
<211>  345
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: Nucleotide sequence of the antigen-binding Fc
       fragment H561-4 CH3 domain

<400>  4
gaaaagacca tctccaaggc caagggccag ccccgcgagc cccaggtgta caccctgccc     60

cctagccggg acgagttctt cacctactgg gtgtccctga cctgcctggt caagggcttc    120

tacccctccg atatcgccgt ggaatgggag tccaacggcc agcccgagaa caactacaag    180

accacccccc ctgtgctgga ctccgacggc tcattcttcc tgtactccaa gctgaccgtg    240

gaccggcgga gatggaccgc cggcaacgtg ttctcctgca gcgtgatgca cgaggccctg    300

cacaaccact acacccagaa gtccctgtcc ctgagccccg gcaag                    345


<210>  5
<211>  15
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: Nucleotide sequence encoding the part of the 
       H561-4 AB structural loop region believed to be involved in 
       antigen binding

<400>  5
ttcttcacct actgg                                                      15


<210>  6
<211>  15
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: Nucleotide sequence encoding part of the 
       H561-4 CD structural loop region

<400>  6
aacggccagc ccgag                                                      15


<210>  7
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: Nucleotide sequence encoding the part of the 
       H561-4 EF structural loop region believed to be involved in 
       antigen binding

<400>  7
gaccggcgga gatggaccgc c                                               21


<210>  8
<211>  223
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: Amino acid sequence of antigen-binding Fc 
       fragment H561-4

<400>  8

Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val 
1               5                   10                  15      


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
            20                  25                  30          


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
        35                  40                  45              


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
    50                  55                  60                  


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
65                  70                  75                  80  


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
                85                  90                  95      


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
            100                 105                 110         


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
        115                 120                 125             


Pro Ser Arg Asp Glu Phe Phe Thr Tyr Trp Val Ser Leu Thr Cys Leu 
    130                 135                 140                 


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
145                 150                 155                 160 


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
                165                 170                 175     


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Arg Arg Arg 
            180                 185                 190         


Trp Thr Ala Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
        195                 200                 205             


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    210                 215                 220             


<210>  9
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: Amino acid sequence of the H561-4 hinge 
       region

<400>  9

Thr Cys Pro Pro Cys Pro 
1               5       


<210>  10
<211>  110
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: Amino acid sequence of the H561-4 CH2 domain

<400>  10

Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
1               5                   10                  15      


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
            20                  25                  30          


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
        35                  40                  45              


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
    50                  55                  60                  


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
65                  70                  75                  80  


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
                85                  90                  95      


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys 
            100                 105                 110 


<210>  11
<211>  107
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: Amino acid sequence of the H561-4 CH3 domain

<400>  11

Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp 
1               5                   10                  15      


Glu Phe Phe Thr Tyr Trp Val Ser Leu Thr Cys Leu Val Lys Gly Phe 
            20                  25                  30          


Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 
        35                  40                  45              


Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 
    50                  55                  60                  


Phe Leu Tyr Ser Lys Leu Thr Val Asp Arg Arg Arg Trp Thr Ala Gly 
65                  70                  75                  80  


Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr 
                85                  90                  95      


Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
            100                 105         


<210>  12
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: Amino acid sequence of the part of the H561-4
       AB structural loop region believed to be involved in antigen 
       binding

<400>  12

Phe Phe Thr Tyr Trp 
1               5   


<210>  13
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: Amino acid sequence of part of the H561-4 CD 
       structural loop region

<400>  13

Asn Gly Gln Pro Glu 
1               5   


<210>  14
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence: Amino acid sequence of the part of the H561-4
       EF structural loop region believed to be involved in antigen 
       binding

<400>  14

Asp Arg Arg Arg Trp Thr Ala 
1               5           


<210>  15
<211>  15
<212>  PRT
<213>  Homo sapiens

<400>  15

Leu Pro Ala Ser Pro Glu Thr His Leu Asp Met Leu Arg His Leu 
1               5                   10                  15  


<210>  16
<211>  15
<212>  PRT
<213>  Homo sapiens

<400>  16

Cys Gln Val Val Gln Gly Asn Leu Glu Leu Thr Tyr Leu Pro Thr 
1               5                   10                  15  


<210>  17
<211>  52
<212>  PRT
<213>  Homo sapiens

<400>  17

Ser Leu Thr Leu Gln Gly Leu Gly Ile Ser Trp Leu Gly Leu Arg Ser 
1               5                   10                  15      


Leu Arg Glu Leu Gly Ser Gly Leu Ala Leu Ile His His Asn Thr His 
            20                  25                  30          


Leu Cys Phe Val His Thr Val Pro Trp Asp Gln Leu Phe Arg Asn Pro 
        35                  40                  45              


His Gln Ala Leu 
    50          


<210>  18
<211>  630
<212>  PRT
<213>  Homo sapiens

<400>  18

Thr Gln Val Cys Thr Gly Thr Asp Met Lys Leu Arg Leu Pro Ala Ser 
1               5                   10                  15      


Pro Glu Thr His Leu Asp Met Leu Arg His Leu Tyr Gln Gly Cys Gln 
            20                  25                  30          


Val Val Gln Gly Asn Leu Glu Leu Thr Tyr Leu Pro Thr Asn Ala Ser 
        35                  40                  45              


Leu Ser Phe Leu Gln Asp Ile Gln Glu Val Gln Gly Tyr Val Leu Ile 
    50                  55                  60                  


Ala His Asn Gln Val Arg Gln Val Pro Leu Gln Arg Leu Arg Ile Val 
65                  70                  75                  80  


Arg Gly Thr Gln Leu Phe Glu Asp Asn Tyr Ala Leu Ala Val Leu Asp 
                85                  90                  95      


Asn Gly Asp Pro Leu Asn Asn Thr Thr Pro Val Thr Gly Ala Ser Pro 
            100                 105                 110         


Gly Gly Leu Arg Glu Leu Gln Leu Arg Ser Leu Thr Glu Ile Leu Lys 
        115                 120                 125             


Gly Gly Val Leu Ile Gln Arg Asn Pro Gln Leu Cys Tyr Gln Asp Thr 
    130                 135                 140                 


Ile Leu Trp Lys Asp Ile Phe His Lys Asn Asn Gln Leu Ala Leu Thr 
145                 150                 155                 160 


Leu Ile Asp Thr Asn Arg Ser Arg Ala Cys His Pro Cys Ser Pro Met 
                165                 170                 175     


Cys Lys Gly Ser Arg Cys Trp Gly Glu Ser Ser Glu Asp Cys Gln Ser 
            180                 185                 190         


Leu Thr Arg Thr Val Cys Ala Gly Gly Cys Ala Arg Cys Lys Gly Pro 
        195                 200                 205             


Leu Pro Thr Asp Cys Cys His Glu Gln Cys Ala Ala Gly Cys Thr Gly 
    210                 215                 220                 


Pro Lys His Ser Asp Cys Leu Ala Cys Leu His Phe Asn His Ser Gly 
225                 230                 235                 240 


Ile Cys Glu Leu His Cys Pro Ala Leu Val Thr Tyr Asn Thr Asp Thr 
                245                 250                 255     


Phe Glu Ser Met Pro Asn Pro Glu Gly Arg Tyr Thr Phe Gly Ala Ser 
            260                 265                 270         


Cys Val Thr Ala Cys Pro Tyr Asn Tyr Leu Ser Thr Asp Val Gly Ser 
        275                 280                 285             


Cys Thr Leu Val Cys Pro Leu His Asn Gln Glu Val Thr Ala Glu Asp 
    290                 295                 300                 


Gly Thr Gln Arg Cys Glu Lys Cys Ser Lys Pro Cys Ala Arg Val Cys 
305                 310                 315                 320 


Tyr Gly Leu Gly Met Glu His Leu Arg Glu Val Arg Ala Val Thr Ser 
                325                 330                 335     


Ala Asn Ile Gln Glu Phe Ala Gly Cys Lys Lys Ile Phe Gly Ser Leu 
            340                 345                 350         


Ala Phe Leu Pro Glu Ser Phe Asp Gly Asp Pro Ala Ser Asn Thr Ala 
        355                 360                 365             


Pro Leu Gln Pro Glu Gln Leu Gln Val Phe Glu Thr Leu Glu Glu Ile 
    370                 375                 380                 


Thr Gly Tyr Leu Tyr Ile Ser Ala Trp Pro Asp Ser Leu Pro Asp Leu 
385                 390                 395                 400 


Ser Val Phe Gln Asn Leu Gln Val Ile Arg Gly Arg Ile Leu His Asn 
                405                 410                 415     


Gly Ala Tyr Ser Leu Thr Leu Gln Gly Leu Gly Ile Ser Trp Leu Gly 
            420                 425                 430         


Leu Arg Ser Leu Arg Glu Leu Gly Ser Gly Leu Ala Leu Ile His His 
        435                 440                 445             


Asn Thr His Leu Cys Phe Val His Thr Val Pro Trp Asp Gln Leu Phe 
    450                 455                 460                 


Arg Asn Pro His Gln Ala Leu Leu His Thr Ala Asn Arg Pro Glu Asp 
465                 470                 475                 480 


Glu Cys Val Gly Glu Gly Leu Ala Cys His Gln Leu Cys Ala Arg Gly 
                485                 490                 495     


His Cys Trp Gly Pro Gly Pro Thr Gln Cys Val Asn Cys Ser Gln Phe 
            500                 505                 510         


Leu Arg Gly Gln Glu Cys Val Glu Glu Cys Arg Val Leu Gln Gly Leu 
        515                 520                 525             


Pro Arg Glu Tyr Val Asn Ala Arg His Cys Leu Pro Cys His Pro Glu 
    530                 535                 540                 


Cys Gln Pro Gln Asn Gly Ser Val Thr Cys Phe Gly Pro Glu Ala Asp 
545                 550                 555                 560 


Gln Cys Val Ala Cys Ala His Tyr Lys Asp Pro Pro Phe Cys Val Ala 
                565                 570                 575     


Arg Cys Pro Ser Gly Val Lys Pro Asp Leu Ser Tyr Met Pro Ile Trp 
            580                 585                 590         


Lys Phe Pro Asp Glu Glu Gly Ala Cys Gln Pro Cys Pro Ile Asn Cys 
        595                 600                 605             


Thr His Ser Cys Val Asp Leu Asp Asp Lys Gly Cys Pro Ala Glu Gln 
    610                 615                 620                 


Arg Ala Ser Pro Leu Thr 
625                 630 


<210>  19
<211>  223
<212>  PRT
<213>  Homo sapiens

<400>  19

Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val 
1               5                   10                  15      


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
            20                  25                  30          


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
        35                  40                  45              


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
    50                  55                  60                  


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
65                  70                  75                  80  


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
                85                  90                  95      


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
            100                 105                 110         


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
        115                 120                 125             


Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
    130                 135                 140                 


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
145                 150                 155                 160 


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
                165                 170                 175     


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
            180                 185                 190         


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
        195                 200                 205             


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    210                 215                 220             

