                         SEQUENCE LISTING

<110>  Max-Planck-Institut fr biophysikalische Chemie
 
<120>  An orthogonal set of tag-cleaving proteases for purification of 
       stoichiometric protein complexes and proteins

<130>  30A-126 358

<160>  28    

<170>  PatentIn version 3.5

<210>  1
<211>  77
<212>  PRT
<213>  Brachypodium distachyon

<400>  1

His Ile Asn Leu Lys Val Lys Gly Gln Asp Gly Asn Glu Val Phe Phe 
1               5                   10                  15      


Arg Ile Lys Arg Ser Thr Gln Leu Lys Lys Leu Met Asn Ala Tyr Cys 
            20                  25                  30          


Asp Arg Gln Ser Val Asp Met Thr Ala Ile Ala Phe Leu Phe Asp Gly 
        35                  40                  45              


Arg Arg Leu Arg Ala Glu Gln Thr Pro Asp Glu Leu Glu Met Glu Asp 
    50                  55                  60                  


Gly Asp Glu Ile Asp Ala Met Leu His Gln Thr Gly Gly 
65                  70                  75          


<210>  2
<211>  234
<212>  PRT
<213>  Brachypodium distachyon

<400>  2

Pro Phe Val Pro Leu Thr Asp Glu Asp Glu Asp Asn Val Arg His Ala 
1               5                   10                  15      


Leu Gly Gly Arg Lys Arg Ser Glu Thr Leu Ser Val His Glu Ala Ser 
            20                  25                  30          


Asn Ile Val Ile Thr Arg Glu Ile Leu Gln Cys Leu Asn Asp Lys Glu 
        35                  40                  45              


Trp Leu Asn Asp Glu Val Ile Asn Leu Tyr Leu Glu Leu Leu Lys Glu 
    50                  55                  60                  


Arg Glu Leu Arg Glu Pro Asn Lys Phe Leu Lys Cys His Phe Phe Asn 
65                  70                  75                  80  


Thr Phe Phe Tyr Lys Lys Leu Ile Asn Gly Gly Tyr Asp Tyr Lys Ser 
                85                  90                  95      


Val Arg Arg Trp Thr Thr Lys Arg Lys Leu Gly Tyr Asn Leu Ile Asp 
            100                 105                 110         


Cys Asp Lys Ile Phe Val Pro Ile His Lys Asp Val His Trp Cys Leu 
        115                 120                 125             


Ala Val Ile Asn Ile Lys Glu Lys Lys Phe Gln Tyr Leu Asp Ser Leu 
    130                 135                 140                 


Gly Tyr Met Asp Met Lys Ala Leu Arg Ile Leu Ala Lys Tyr Leu Val 
145                 150                 155                 160 


Asp Glu Val Lys Asp Lys Ser Gly Lys Gln Ile Asp Val His Ala Trp 
                165                 170                 175     


Lys Gln Glu Gly Val Gln Asn Leu Pro Leu Gln Glu Asn Gly Trp Asp 
            180                 185                 190         


Cys Gly Met Phe Met Leu Lys Tyr Ile Asp Phe Tyr Ser Arg Asp Met 
        195                 200                 205             


Glu Leu Val Phe Gly Gln Lys His Met Ser Tyr Phe Arg Arg Arg Thr 
    210                 215                 220                 


Ala Lys Glu Ile Leu Asp Leu Lys Ala Gly 
225                 230                 


<210>  3
<211>  76
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  3

His Ile Asn Leu Lys Val Ser Asp Gly Ser Ser Glu Ile Phe Phe Lys 
1               5                   10                  15      


Ile Lys Lys Thr Thr Pro Leu Arg Arg Leu Met Glu Ala Phe Ala Lys 
            20                  25                  30          


Arg Gln Gly Lys Glu Met Asp Ser Leu Arg Phe Leu Tyr Asp Gly Ile 
        35                  40                  45              


Arg Ile Gln Ala Asp Gln Thr Pro Glu Asp Leu Asp Met Glu Asp Asn 
    50                  55                  60                  


Asp Ile Ile Glu Ala His Arg Glu Gln Ile Gly Gly 
65                  70                  75      


<210>  4
<211>  219
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  4

Leu Val Pro Glu Leu Asn Glu Lys Asp Asp Asp Gln Val Gln Lys Ala 
1               5                   10                  15      


Leu Ala Ser Arg Glu Asn Thr Gln Leu Met Asn Arg Asp Asn Ile Glu 
            20                  25                  30          


Ile Thr Val Arg Asp Phe Lys Thr Leu Ala Pro Arg Arg Trp Leu Asn 
        35                  40                  45              


Asp Thr Ile Ile Glu Phe Phe Met Lys Tyr Ile Glu Lys Ser Thr Pro 
    50                  55                  60                  


Asn Thr Val Ala Phe Asn Ser Phe Phe Tyr Thr Asn Leu Ser Glu Arg 
65                  70                  75                  80  


Gly Tyr Gln Gly Val Arg Arg Trp Met Lys Arg Lys Lys Thr Gln Ile 
                85                  90                  95      


Asp Lys Leu Asp Lys Ile Phe Thr Pro Ile Asn Leu Asn Gln Ser His 
            100                 105                 110         


Trp Ala Leu Gly Ile Ile Asp Leu Lys Lys Lys Thr Ile Gly Tyr Val 
        115                 120                 125             


Asp Ser Leu Ser Asn Gly Pro Asn Ala Met Ser Phe Ala Ile Leu Thr 
    130                 135                 140                 


Asp Leu Gln Lys Tyr Val Met Glu Glu Ser Lys His Thr Ile Gly Glu 
145                 150                 155                 160 


Asp Phe Asp Leu Ile His Leu Asp Cys Pro Gln Gln Pro Asn Gly Tyr 
                165                 170                 175     


Asp Cys Gly Ile Tyr Val Cys Met Asn Thr Leu Tyr Gly Ser Ala Asp 
            180                 185                 190         


Ala Pro Leu Asp Phe Asp Tyr Lys Asp Ala Ile Arg Met Arg Arg Phe 
        195                 200                 205             


Ile Ala His Leu Ile Leu Thr Asp Ala Leu Lys 
    210                 215                 


<210>  5
<211>  77
<212>  PRT
<213>  Homo sapiens

<400>  5

Tyr Ile Lys Leu Lys Val Ile Gly Gln Asp Ser Ser Glu Ile His Phe 
1               5                   10                  15      


Lys Val Lys Met Thr Thr His Leu Lys Lys Leu Lys Glu Ser Tyr Cys 
            20                  25                  30          


Gln Arg Gln Gly Val Pro Met Asn Ser Leu Arg Phe Leu Phe Glu Gly 
        35                  40                  45              


Gln Arg Ile Ala Asp Asn His Thr Pro Lys Glu Leu Gly Met Glu Glu 
    50                  55                  60                  


Glu Asp Val Ile Glu Val Tyr Gln Glu Gln Thr Gly Gly 
65                  70                  75          


<210>  6
<211>  77
<212>  PRT
<213>  Homo sapiens

<400>  6

His Ile Asn Leu Lys Val Ala Gly Gln Asp Gly Ser Val Val Gln Phe 
1               5                   10                  15      


Lys Ile Lys Arg His Thr Pro Leu Ser Lys Leu Met Lys Ala Tyr Cys 
            20                  25                  30          


Glu Arg Gln Gly Leu Ser Met Arg Gln Ile Arg Phe Arg Phe Asp Gly 
        35                  40                  45              


Gln Pro Ile Asn Glu Thr Asp Thr Pro Ala Gln Leu Glu Met Glu Asp 
    50                  55                  60                  


Glu Asp Thr Ile Asp Val Phe Gln Gln Gln Thr Gly Gly 
65                  70                  75          


<210>  7
<211>  226
<212>  PRT
<213>  Homo sapiens

<400>  7

Glu Phe Pro Glu Ile Thr Glu Glu Met Glu Lys Glu Ile Lys Asn Val 
1               5                   10                  15      


Phe Arg Asn Gly Asn Gln Asp Glu Val Leu Ser Glu Ala Phe Arg Leu 
            20                  25                  30          


Thr Ile Thr Arg Lys Asp Ile Gln Thr Leu Asn His Leu Asn Trp Leu 
        35                  40                  45              


Asn Asp Glu Ile Ile Asn Phe Tyr Met Asn Met Leu Met Glu Arg Ser 
    50                  55                  60                  


Lys Glu Lys Gly Leu Pro Ser Val His Ala Phe Asn Thr Phe Phe Phe 
65                  70                  75                  80  


Thr Lys Leu Lys Thr Ala Gly Tyr Gln Ala Val Lys Arg Trp Thr Lys 
                85                  90                  95      


Lys Val Asp Val Phe Ser Val Asp Ile Leu Leu Val Pro Ile His Leu 
            100                 105                 110         


Gly Val His Trp Cys Leu Ala Val Val Asp Phe Arg Lys Lys Asn Ile 
        115                 120                 125             


Thr Tyr Tyr Asp Ser Met Gly Gly Ile Asn Asn Glu Ala Cys Arg Ile 
    130                 135                 140                 


Leu Leu Gln Tyr Leu Lys Gln Glu Ser Ile Asp Lys Lys Arg Lys Glu 
145                 150                 155                 160 


Phe Asp Thr Asn Gly Trp Gln Leu Phe Ser Lys Lys Ser Gln Glu Ile 
                165                 170                 175     


Pro Gln Gln Met Asn Gly Ser Asp Cys Gly Met Phe Ala Cys Lys Tyr 
            180                 185                 190         


Ala Asp Cys Ile Thr Lys Asp Arg Pro Ile Asn Phe Thr Gln Gln His 
        195                 200                 205             


Met Pro Tyr Phe Arg Lys Arg Met Val Trp Glu Ile Leu His Arg Lys 
    210                 215                 220                 


Leu Leu 
225     


<210>  8
<211>  76
<212>  PRT
<213>  Salmo salar

<400>  8

Met Leu Ile Lys Val Lys Thr Leu Thr Gly Lys Glu Ile Glu Ile Asp 
1               5                   10                  15      


Ile Glu Pro Thr Asp Lys Val Glu Arg Ile Lys Glu Arg Val Glu Glu 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Gln Gln Gln Arg Leu Ile Tyr Ser Gly Lys 
        35                  40                  45              


Gln Met Asn Asp Glu Lys Thr Ala Ala Asp Tyr Lys Ile Gln Gly Gly 
    50                  55                  60                  


Ser Val Leu His Leu Val Leu Ala Leu Arg Gly Gly 
65                  70                  75      


<210>  9
<211>  75
<212>  PRT
<213>  Brachypodium distachyon

<400>  9

Met Ile Lys Val Lys Thr Leu Thr Gly Lys Glu Ile Glu Ile Asp Ile 
1               5                   10                  15      


Glu Pro Thr Asp Thr Ile Asp Arg Ile Lys Glu Arg Val Glu Glu Lys 
            20                  25                  30          


Glu Gly Ile Pro Pro Val Gln Gln Arg Leu Ile Tyr Ala Gly Lys Gln 
        35                  40                  45              


Leu Ala Asp Asp Lys Thr Ala Lys Asp Tyr Asn Ile Glu Gly Gly Ser 
    50                  55                  60                  


Val Leu His Leu Val Leu Ala Leu Arg Gly Gly 
65                  70                  75  


<210>  10
<211>  76
<212>  PRT
<213>  Homo sapiens

<400>  10

Met Leu Ile Lys Val Lys Thr Leu Thr Gly Lys Glu Ile Glu Ile Asp 
1               5                   10                  15      


Ile Glu Pro Thr Asp Lys Val Glu Arg Ile Lys Glu Arg Val Glu Glu 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Gln Gln Gln Arg Leu Ile Tyr Ser Gly Lys 
        35                  40                  45              


Gln Met Asn Asp Glu Lys Thr Ala Ala Asp Tyr Lys Ile Leu Gly Gly 
    50                  55                  60                  


Ser Val Leu His Leu Val Leu Ala Leu Arg Gly Gly 
65                  70                  75      


<210>  11
<211>  212
<212>  PRT
<213>  Salmo salar

<400>  11

Met Asp Pro Val Val Leu Ser Tyr Gln Asp Ser Leu Leu Arg Arg Ser 
1               5                   10                  15      


Asp Val Ala Leu Leu Glu Gly Pro His Trp Leu Asn Asp Gln Val Ile 
            20                  25                  30          


Gly Phe Ala Phe Glu Tyr Phe Ala Ala Glu Leu Phe Lys Gly Leu Gly 
        35                  40                  45              


Glu Ala Ala Ile Phe Ile Ser Pro Glu Val Thr Gln Phe Ile Lys Cys 
    50                  55                  60                  


Ala Ala Cys Pro Glu Asp Leu Ala Leu Phe Leu Glu Pro Leu Gly Leu 
65                  70                  75                  80  


Ala Ser Arg Arg Trp Val Phe Leu Ala Val Asn Asp Asn Ser Ile Gln 
                85                  90                  95      


Thr Ala Gly Gly Ser His Trp Ser Leu Leu Leu Phe Leu Arg Asp Ser 
            100                 105                 110         


Gly His Phe Ala His Tyr Asp Ser Gln Ser Gly Gly Asn Ser Leu His 
        115                 120                 125             


Ala Arg Arg Ile Ala Thr Lys Leu Glu Pro Phe Leu Gly Ser Gly Arg 
    130                 135                 140                 


Lys Val Pro Phe Val Glu Glu Pro Cys Pro Leu Gln Gln Asn Ser Tyr 
145                 150                 155                 160 


Asp Cys Gly Met Tyr Val Ile Cys Asn Ala Glu Ala Leu Cys Glu Arg 
                165                 170                 175     


Ala Arg Val Glu Gly Ser Pro Arg Leu Pro Val Gln Thr Ile Thr Pro 
            180                 185                 190         


Ala Tyr Ile Thr Gln Lys Arg Leu Glu Trp Cys Arg Leu Ile Gln Arg 
        195                 200                 205             


Leu Asp Arg Asp 
    210         


<210>  12
<211>  242
<212>  PRT
<213>  Brachypodium distachyon

<400>  12

Met Asp Glu Arg Val Leu Ser Tyr Gly Asp Val Val Leu Leu Arg Ser 
1               5                   10                  15      


Asp Leu Ala Ile Leu Arg Gly Pro His Phe Leu Asn Asp Arg Ile Ile 
            20                  25                  30          


Ala Phe Tyr Leu Ala His Leu Ser Ala Ser Phe His Gly Asp Gly Asp 
        35                  40                  45              


Leu Leu Leu Leu Pro Pro Ser Ile Pro Tyr Leu Leu Ser Asn Leu Pro 
    50                  55                  60                  


Asp Pro Glu Ser Val Ala Glu Pro Leu Cys Leu Ala Ser Arg Arg Leu 
65                  70                  75                  80  


Val Leu Leu Pro Val Asn Asp Asn Pro Asp Ala Ser Val Ala Asn Gly 
                85                  90                  95      


Gly Ser His Trp Thr Leu Leu Val Leu Asp Ala Ala Thr Thr Asp Pro 
            100                 105                 110         


Gln Ala Pro Arg Phe Val His His Asp Ser Leu Arg Gly Ser Ala Asn 
        115                 120                 125             


Ala Ala Ala Ala Arg Arg Leu Ala Arg Ala Leu Thr Ala Gly Gly Ala 
    130                 135                 140                 


Pro Leu Arg Phe Val Glu Ala Pro Thr Pro Thr Gln Arg Asn Gly His 
145                 150                 155                 160 


Asp Cys Gly Val Tyr Val Leu Ala Val Ala Arg Ala Ile Cys Gly Trp 
                165                 170                 175     


Trp Arg Ser Ser Arg Arg Arg Glu Asn Gln Gln Gly Gly Gly Gly Asp 
            180                 185                 190         


Trp Phe Ala Thr Met Met Glu Glu Val Asp Ala Glu Ser Val Gly Ala 
        195                 200                 205             


Met Arg Ala Glu Leu Leu Gln Leu Ile His Arg Leu Ile Gln Asp Lys 
    210                 215                 220                 


Glu Gln Glu Glu Glu Lys Lys Ser Lys Ala Gly Val Glu Asp Thr Cys 
225                 230                 235                 240 


Gly Gln 
        


<210>  13
<211>  212
<212>  PRT
<213>  Homo sapiens

<400>  13

Met Asp Pro Val Val Leu Ser Tyr Met Asp Ser Leu Leu Arg Gln Ser 
1               5                   10                  15      


Asp Val Ser Leu Leu Asp Pro Pro Ser Trp Leu Asn Asp His Ile Ile 
            20                  25                  30          


Gly Phe Ala Phe Glu Tyr Phe Ala Asn Ser Gln Phe His Asp Cys Ser 
        35                  40                  45              


Asp His Val Ser Phe Ile Ser Pro Glu Val Thr Gln Phe Ile Lys Cys 
    50                  55                  60                  


Thr Ser Asn Pro Ala Glu Ile Ala Met Phe Leu Glu Pro Leu Asp Leu 
65                  70                  75                  80  


Pro Asn Lys Arg Val Val Phe Leu Ala Ile Asn Asp Asn Ser Asn Gln 
                85                  90                  95      


Ala Ala Gly Gly Thr His Trp Ser Leu Leu Val Tyr Leu Gln Asp Lys 
            100                 105                 110         


Asn Ser Phe Phe His Tyr Asp Ser His Ser Arg Ser Asn Ser Val His 
        115                 120                 125             


Ala Lys Gln Val Ala Glu Lys Leu Glu Ala Phe Leu Gly Arg Lys Gly 
    130                 135                 140                 


Asp Lys Leu Ala Phe Val Glu Glu Lys Ala Pro Ala Gln Gln Asn Ser 
145                 150                 155                 160 


Tyr Asp Cys Gly Met Tyr Val Ile Cys Asn Thr Glu Ala Leu Cys Gln 
                165                 170                 175     


Asn Phe Phe Arg Gln Gln Thr Glu Ser Leu Leu Gln Leu Leu Thr Pro 
            180                 185                 190         


Ala Tyr Ile Thr Lys Lys Arg Gly Glu Trp Lys Asp Leu Ile Thr Thr 
        195                 200                 205             


Leu Ala Lys Lys 
    210         


<210>  14
<211>  116
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  14

Met Lys Ser Thr Phe Lys Ser Glu Tyr Pro Phe Glu Lys Arg Lys Ala 
1               5                   10                  15      


Glu Ser Glu Arg Ile Ala Asp Arg Phe Lys Asn Arg Ile Pro Val Ile 
            20                  25                  30          


Cys Glu Lys Ala Glu Lys Ser Asp Ile Pro Glu Ile Asp Lys Arg Lys 
        35                  40                  45              


Tyr Leu Val Pro Ala Asp Leu Thr Val Gly Gln Phe Val Tyr Val Ile 
    50                  55                  60                  


Arg Lys Arg Ile Met Leu Pro Pro Glu Lys Ala Ile Phe Ile Phe Val 
65                  70                  75                  80  


Asn Asp Thr Leu Pro Pro Thr Ala Ala Leu Met Ser Ala Ile Tyr Gln 
                85                  90                  95      


Glu His Lys Asp Lys Asp Gly Phe Leu Tyr Val Thr Tyr Ser Gly Glu 
            100                 105                 110         


Asn Thr Phe Gly 
        115     


<210>  15
<211>  494
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  15

Met Gln Arg Trp Leu Gln Leu Trp Lys Met Asp Leu Val Gln Lys Val 
1               5                   10                  15      


Ser His Gly Val Phe Glu Gly Ser Ser Glu Glu Pro Ala Ala Leu Met 
            20                  25                  30          


Asn His Asp Tyr Ile Val Leu Gly Glu Val Tyr Pro Glu Arg Asp Glu 
        35                  40                  45              


Glu Ser Gly Ala Glu Gln Cys Glu Gln Asp Cys Arg Tyr Arg Gly Glu 
    50                  55                  60                  


Ala Val Ser Asp Gly Phe Leu Ser Ser Leu Phe Gly Arg Glu Ile Ser 
65                  70                  75                  80  


Ser Tyr Thr Lys Glu Phe Leu Leu Asp Val Gln Ser Arg Val Asn Phe 
                85                  90                  95      


Thr Tyr Arg Thr Arg Phe Val Pro Ile Ala Arg Ala Pro Asp Gly Pro 
            100                 105                 110         


Ser Pro Leu Ser Leu Asn Leu Leu Val Arg Thr Asn Pro Ile Ser Thr 
        115                 120                 125             


Ile Glu Asp Tyr Ile Ala Asn Pro Asp Cys Phe Asn Thr Asp Ile Gly 
    130                 135                 140                 


Trp Gly Cys Met Ile Arg Thr Gly Gln Ser Leu Leu Gly Asn Ala Leu 
145                 150                 155                 160 


Gln Ile Leu His Leu Gly Arg Asp Phe Arg Val Asn Gly Asn Glu Ser 
                165                 170                 175     


Leu Glu Arg Glu Ser Lys Phe Val Asn Trp Phe Asn Asp Thr Pro Glu 
            180                 185                 190         


Ala Pro Phe Ser Leu His Asn Phe Val Ser Ala Gly Thr Glu Leu Ser 
        195                 200                 205             


Asp Lys Arg Pro Gly Glu Trp Phe Gly Pro Ala Ala Thr Ala Arg Ser 
    210                 215                 220                 


Ile Gln Ser Leu Ile Tyr Gly Phe Pro Glu Cys Gly Ile Asp Asp Cys 
225                 230                 235                 240 


Ile Val Ser Val Ser Ser Gly Asp Ile Tyr Glu Asn Glu Val Glu Lys 
                245                 250                 255     


Val Phe Ala Glu Asn Pro Asn Ser Arg Ile Leu Phe Leu Leu Gly Val 
            260                 265                 270         


Lys Leu Gly Ile Asn Ala Val Asn Glu Ser Tyr Arg Glu Ser Ile Cys 
        275                 280                 285             


Gly Ile Leu Ser Ser Thr Gln Ser Val Gly Ile Ala Gly Gly Arg Pro 
    290                 295                 300                 


Ser Ser Ser Leu Tyr Phe Phe Gly Tyr Gln Gly Asn Glu Phe Leu His 
305                 310                 315                 320 


Phe Asp Pro His Ile Pro Gln Pro Ala Val Glu Asp Ser Phe Val Glu 
                325                 330                 335     


Ser Cys His Thr Ser Lys Phe Gly Lys Leu Gln Leu Ser Glu Met Asp 
            340                 345                 350         


Pro Ser Met Leu Ile Gly Ile Leu Ile Lys Gly Glu Lys Asp Trp Gln 
        355                 360                 365             


Gln Trp Lys Leu Glu Val Ala Glu Ser Ala Ile Ile Asn Val Leu Ala 
    370                 375                 380                 


Lys Arg Met Asp Asp Phe Asp Val Ser Cys Ser Met Asp Asp Val Glu 
385                 390                 395                 400 


Ser Val Ser Ser Asn Ser Met Lys Lys Asp Ala Ser Asn Asn Glu Asn 
                405                 410                 415     


Leu Gly Val Leu Glu Gly Asp Tyr Val Asp Ile Gly Ala Ile Phe Pro 
            420                 425                 430         


His Thr Thr Asn Thr Glu Asp Val Asp Glu Tyr Asp Cys Phe Gln Asp 
        435                 440                 445             


Ile His Cys Lys Lys Gln Lys Ile Val Val Met Gly Asn Thr His Thr 
    450                 455                 460                 


Val Asn Ala Asn Leu Thr Asp Tyr Glu Val Glu Gly Val Leu Val Glu 
465                 470                 475                 480 


Lys Glu Thr Val Gly Ile His Ser Pro Ile Asp Glu Lys Cys 
                485                 490                 


<210>  16
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  TEV PRS in Fig. 4A


<220>
<221>  MISC_FEATURE
<222>  (7)..(8)
<223>  TEV protease recognition site

<400>  16

Glu Asn Leu Tyr Phe Gln Gly Thr 
1               5               


<210>  17
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  TEV PRS in Fig. 12A


<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  wherein Xaa = none, Met, Tyr, Arg, Glu or Pro

<220>
<221>  MISC_FEATURE
<222>  (7)..(9)
<223>  TEV protease recognition site

<400>  17

Glu Asn Leu Tyr Phe Gln Xaa Gly Thr 
1               5                   


<210>  18
<211>  242
<212>  PRT
<213>  Tobacco etch virus


<220>
<221>  MISC_FEATURE
<222>  (237)..(242)
<223>  C-terminal autoinhibitory peptide

<400>  18

Gly Glu Ser Leu Phe Lys Gly Pro Arg Asp Tyr Asn Pro Ile Ser Ser 
1               5                   10                  15      


Thr Ile Cys His Leu Thr Asn Glu Ser Asp Gly His Thr Thr Ser Leu 
            20                  25                  30          


Tyr Gly Ile Gly Phe Gly Pro Phe Ile Ile Thr Asn Lys His Leu Phe 
        35                  40                  45              


Arg Arg Asn Asn Gly Thr Leu Leu Val Gln Ser Leu His Gly Val Phe 
    50                  55                  60                  


Lys Val Lys Asn Thr Thr Thr Leu Gln Gln His Leu Ile Asp Gly Arg 
65                  70                  75                  80  


Asp Met Ile Ile Ile Arg Met Pro Lys Asp Phe Pro Pro Phe Pro Gln 
                85                  90                  95      


Lys Leu Lys Phe Arg Glu Pro Gln Arg Glu Glu Arg Ile Cys Leu Val 
            100                 105                 110         


Thr Thr Asn Phe Gln Thr Lys Ser Met Ser Ser Met Val Ser Asp Thr 
        115                 120                 125             


Ser Cys Thr Phe Pro Ser Ser Asp Gly Ile Phe Trp Lys His Trp Ile 
    130                 135                 140                 


Gln Thr Lys Asp Gly Gln Cys Gly Ser Pro Leu Val Ser Thr Arg Asp 
145                 150                 155                 160 


Gly Phe Ile Val Gly Ile His Ser Ala Ser Asn Phe Thr Asn Thr Asn 
                165                 170                 175     


Asn Tyr Phe Thr Ser Val Pro Lys Asn Phe Met Glu Leu Leu Thr Asn 
            180                 185                 190         


Gln Glu Ala Gln Gln Trp Val Ser Gly Trp Arg Leu Asn Ala Asp Ser 
        195                 200                 205             


Val Leu Trp Gly Gly His Lys Val Phe Met Ser Lys Pro Glu Glu Pro 
    210                 215                 220                 


Phe Gln Pro Val Lys Glu Ala Thr Gln Leu Met Asn Glu Leu Val Tyr 
225                 230                 235                 240 


Ser Gln 
        


<210>  19
<211>  235
<212>  PRT
<213>  Artificial

<220>
<223>  TEV(SH) variant

<400>  19

Glu Ser Leu Phe Lys Gly Pro Arg Asp Tyr Asn Pro Ile Ser Ser Ser 
1               5                   10                  15      


Ile Cys His Leu Thr Asn Glu Ser Asp Gly His Thr Thr Ser Leu Tyr 
            20                  25                  30          


Gly Ile Gly Phe Gly Pro Phe Ile Ile Thr Asn Lys His Leu Phe Arg 
        35                  40                  45              


Arg Asn Asn Gly Thr Leu Leu Val Gln Ser Leu His Gly Val Phe Lys 
    50                  55                  60                  


Val Lys Asp Thr Thr Thr Leu Gln Gln His Leu Val Asp Gly Arg Asp 
65                  70                  75                  80  


Met Ile Ile Ile Arg Met Pro Lys Asp Phe Pro Pro Phe Pro Gln Lys 
                85                  90                  95      


Leu Lys Phe Arg Glu Pro Gln Arg Glu Glu Arg Ile Cys Leu Val Thr 
            100                 105                 110         


Thr Asn Phe Gln Thr Lys Ser Met Ser Ser Met Val Ser Asp Thr Ser 
        115                 120                 125             


Cys Thr Phe Pro Ser Ser Asp Gly Ile Phe Trp Lys His Trp Ile Gln 
    130                 135                 140                 


Thr Lys Asp Gly Gln Cys Gly Ser Pro Leu Val Ser Thr Arg Asp Gly 
145                 150                 155                 160 


Phe Ile Val Gly Ile His Ser Ala Ser Asn Phe Thr Asn Thr Asn Asn 
                165                 170                 175     


Tyr Phe Thr Ser Val Pro Lys Asn Phe Met Glu Leu Leu Thr Asn Gln 
            180                 185                 190         


Glu Ala Gln Gln Trp Val Ser Gly Trp Arg Leu Asn Ala Asp Ser Val 
        195                 200                 205             


Leu Trp Gly Gly His Lys Val Phe Met Asn Lys Pro Glu Glu Pro Phe 
    210                 215                 220                 


Gln Pro Val Lys Glu Ala Thr Gln Leu Met Asn 
225                 230                 235 


<210>  20
<211>  76
<212>  PRT
<213>  Xenopus laevis

<400>  20

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly 
65                  70                  75      


<210>  21
<211>  383
<212>  PRT
<213>  Xenopus laevis

<400>  21

Met Arg Ser His Thr Leu Arg Ile His Gly Met Gly Ala Gly Arg Glu 
1               5                   10                  15      


His Gln Ile Pro Gly Thr Val Ile Leu Ser Ser Ile Met Asp Phe Ile 
            20                  25                  30          


Leu His Arg Ala Lys Ser Ser Lys His Val Gln Gly Leu Val Gly Leu 
        35                  40                  45              


Arg Asn Leu Gly Asn Thr Cys Phe Met Asn Ser Ile Leu Gln Cys Leu 
    50                  55                  60                  


Ser Asn Thr Lys Asp Leu Arg Asp Tyr Cys Gln Gln Asn Ser Tyr Arg 
65                  70                  75                  80  


Arg Asp Leu Ser Ser Lys Lys Cys Asn Thr Ala Ile Met Glu Glu Phe 
                85                  90                  95      


Ala Arg Leu Leu Gln Ala Ile Trp Thr Ser Ser Ala Asn Glu Val Val 
            100                 105                 110         


Ser Pro Ser Glu Phe Lys Thr Gln Ile Gln Arg Tyr Ala Pro Arg Phe 
        115                 120                 125             


Met Gly Tyr Asn Gln Gln Asp Ala Gln Glu Phe Leu Arg Phe Leu Leu 
    130                 135                 140                 


Asp Gly Leu His Asn Glu Val Asn Arg Val Thr Val Lys Pro Arg Pro 
145                 150                 155                 160 


Ser Ser Gln Asp Leu Asp His Met Pro Asp Ser Glu Lys Gly Lys Lys 
                165                 170                 175     


Met Trp Lys Arg Tyr Leu Glu Arg Glu Asp Ser Arg Ile Val Glu Leu 
            180                 185                 190         


Phe Val Gly Gln Leu Lys Ser Ser Leu Thr Cys Thr Asp Cys Gly Tyr 
        195                 200                 205             


Cys Ser Thr Val Phe Asp Pro Phe Trp Asp Leu Ser Leu Pro Ile Ala 
    210                 215                 220                 


Lys Lys Ser Ala Ser Glu Val Ser Leu Val Asp Cys Met Arg Leu Phe 
225                 230                 235                 240 


Thr Lys Glu Asp Val Leu Asp Gly Asp Glu Lys Pro Thr Cys Cys Arg 
                245                 250                 255     


Cys Lys Ala Arg Arg Arg Cys Thr Lys Lys Phe Thr Ile Gln Arg Phe 
            260                 265                 270         


Pro Lys Ile Leu Val Leu His Leu Lys Arg Phe Ser Glu Gly Arg Ile 
        275                 280                 285             


Arg Ser Gly Lys Leu Ser Thr Phe Val Asn Phe Pro Leu Lys Asp Leu 
    290                 295                 300                 


Asp Leu Arg Glu Phe Ser Ser Glu Ser Asn Pro His Ala Thr Tyr Asn 
305                 310                 315                 320 


Leu Tyr Ala Val Ser Asn His Ser Gly Thr Thr Met Gly Gly His Tyr 
                325                 330                 335     


Thr Ala Tyr Cys Lys Asn Pro Ser Asn Gly Glu Trp Tyr Thr Phe Asn 
            340                 345                 350         


Asp Ser Arg Val Thr Ala Met Ser Ser Ser Gln Val Lys Ser Ser Asp 
        355                 360                 365             


Ala Tyr Val Leu Phe Tyr Glu Leu Ser Gly Pro Ser Ser Arg Met 
    370                 375                 380             


<210>  22
<211>  496
<212>  PRT
<213>  Artificial

<220>
<223>  His14-bdSUMO-MBP

<400>  22

Met Ser Lys His His His His Ser Gly His His His Thr Gly His His 
1               5                   10                  15      


His His Ser Gly Ser His His His Ser Gly Ser Ala Ala Gly Gly Glu 
            20                  25                  30          


Glu Asp Lys Lys Pro Ala Gly Gly Glu Gly Gly Gly Ala His Ile Asn 
        35                  40                  45              


Leu Lys Val Lys Gly Gln Asp Gly Asn Glu Val Phe Phe Arg Ile Lys 
    50                  55                  60                  


Arg Ser Thr Gln Leu Lys Lys Leu Met Asn Ala Tyr Cys Asp Arg Gln 
65                  70                  75                  80  


Ser Val Asp Met Thr Ala Ile Ala Phe Leu Phe Asp Gly Arg Arg Leu 
                85                  90                  95      


Arg Ala Glu Gln Thr Pro Asp Glu Leu Glu Met Glu Asp Gly Asp Glu 
            100                 105                 110         


Ile Asp Ala Met Leu His Gln Thr Gly Gly Ala Gly Thr Lys Thr Glu 
        115                 120                 125             


Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys Gly Tyr Asn Gly 
    130                 135                 140                 


Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr Gly Ile Lys Val 
145                 150                 155                 160 


Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe Pro Gln Val Ala 
                165                 170                 175     


Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala His Asp Arg Phe 
            180                 185                 190         


Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile Thr Pro Asp Lys 
        195                 200                 205             


Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp Ala Val Arg Tyr 
    210                 215                 220                 


Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu Ala Leu Ser Leu 
225                 230                 235                 240 


Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys Thr Trp Glu Glu 
                245                 250                 255     


Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly Lys Ser Ala Leu 
            260                 265                 270         


Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro Leu Ile Ala Ala 
        275                 280                 285             


Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys Tyr Asp Ile Lys 
    290                 295                 300                 


Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly Leu Thr Phe Leu 
305                 310                 315                 320 


Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp Thr Asp Tyr Ser 
                325                 330                 335     


Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala Met Thr Ile Asn 
            340                 345                 350         


Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys Val Asn Tyr Gly 
        355                 360                 365             


Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser Lys Pro Phe Val 
    370                 375                 380                 


Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro Asn Lys Glu Leu 
385                 390                 395                 400 


Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp Glu Gly Leu Glu 
                405                 410                 415     


Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala Leu Lys Ser Tyr 
            420                 425                 430         


Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala Thr Met Glu Asn 
        435                 440                 445             


Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln Met Ser Ala Phe 
    450                 455                 460                 


Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala Ser Gly Arg Gln 
465                 470                 475                 480 


Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn Gly Thr Gly Cys 
                485                 490                 495     


<210>  23
<211>  512
<212>  PRT
<213>  Artificial

<220>
<223>  His10-ZZ-TEV-MBP

<400>  23

Met His His His His His His His His His His Gly Ser Asn Lys Phe 
1               5                   10                  15      


Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn 
            20                  25                  30          


Leu Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys Asp Asp 
        35                  40                  45              


Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn Asp 
    50                  55                  60                  


Ala Gln Ala Pro Lys Val Ala Met Asn Lys Phe Asn Lys Glu Gln Gln 
65                  70                  75                  80  


Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Asn Glu Glu Gln 
                85                  90                  95      


Arg Asn Ala Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala 
            100                 105                 110         


Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys 
        115                 120                 125             


Val Ala Met Ser Gly Glu Asn Leu Tyr Phe Gln Gly Thr Lys Thr Glu 
    130                 135                 140                 


Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys Gly Tyr Asn Gly 
145                 150                 155                 160 


Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr Gly Ile Lys Val 
                165                 170                 175     


Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe Pro Gln Val Ala 
            180                 185                 190         


Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala His Asp Arg Phe 
        195                 200                 205             


Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile Thr Pro Asp Lys 
    210                 215                 220                 


Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp Ala Val Arg Tyr 
225                 230                 235                 240 


Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu Ala Leu Ser Leu 
                245                 250                 255     


Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys Thr Trp Glu Glu 
            260                 265                 270         


Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly Lys Ser Ala Leu 
        275                 280                 285             


Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro Leu Ile Ala Ala 
    290                 295                 300                 


Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys Tyr Asp Ile Lys 
305                 310                 315                 320 


Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly Leu Thr Phe Leu 
                325                 330                 335     


Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp Thr Asp Tyr Ser 
            340                 345                 350         


Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala Met Thr Ile Asn 
        355                 360                 365             


Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys Val Asn Tyr Gly 
    370                 375                 380                 


Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser Lys Pro Phe Val 
385                 390                 395                 400 


Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro Asn Lys Glu Leu 
                405                 410                 415     


Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp Glu Gly Leu Glu 
            420                 425                 430         


Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala Leu Lys Ser Tyr 
        435                 440                 445             


Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala Thr Met Glu Asn 
    450                 455                 460                 


Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln Met Ser Ala Phe 
465                 470                 475                 480 


Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala Ser Gly Arg Gln 
                485                 490                 495     


Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn Gly Thr Gly Cys 
            500                 505                 510         


<210>  24
<211>  476
<212>  PRT
<213>  Artificial

<220>
<223>  His14-bdNEDD8-MBP

<400>  24

Met Ser Lys His His His His Ser Gly His His His Thr Gly His His 
1               5                   10                  15      


His His Ser Gly Ser His His His Ser Gly Thr Met Ile Lys Val Lys 
            20                  25                  30          


Thr Leu Thr Gly Lys Glu Ile Glu Ile Asp Ile Glu Pro Thr Asp Thr 
        35                  40                  45              


Ile Asp Arg Ile Lys Glu Arg Val Glu Glu Lys Glu Gly Ile Pro Pro 
    50                  55                  60                  


Val Gln Gln Arg Leu Ile Tyr Ala Gly Lys Gln Leu Ala Asp Asp Lys 
65                  70                  75                  80  


Thr Ala Lys Asp Tyr Asn Ile Glu Gly Gly Ser Val Leu His Leu Val 
                85                  90                  95      


Leu Ala Leu Arg Gly Gly Ala Gly Thr Lys Thr Glu Glu Gly Lys Leu 
            100                 105                 110         


Val Ile Trp Ile Asn Gly Asp Lys Gly Tyr Asn Gly Leu Ala Glu Val 
        115                 120                 125             


Gly Lys Lys Phe Glu Lys Asp Thr Gly Ile Lys Val Thr Val Glu His 
    130                 135                 140                 


Pro Asp Lys Leu Glu Glu Lys Phe Pro Gln Val Ala Ala Thr Gly Asp 
145                 150                 155                 160 


Gly Pro Asp Ile Ile Phe Trp Ala His Asp Arg Phe Gly Gly Tyr Ala 
                165                 170                 175     


Gln Ser Gly Leu Leu Ala Glu Ile Thr Pro Asp Lys Ala Phe Gln Asp 
            180                 185                 190         


Lys Leu Tyr Pro Phe Thr Trp Asp Ala Val Arg Tyr Asn Gly Lys Leu 
        195                 200                 205             


Ile Ala Tyr Pro Ile Ala Val Glu Ala Leu Ser Leu Ile Tyr Asn Lys 
    210                 215                 220                 


Asp Leu Leu Pro Asn Pro Pro Lys Thr Trp Glu Glu Ile Pro Ala Leu 
225                 230                 235                 240 


Asp Lys Glu Leu Lys Ala Lys Gly Lys Ser Ala Leu Met Phe Asn Leu 
                245                 250                 255     


Gln Glu Pro Tyr Phe Thr Trp Pro Leu Ile Ala Ala Asp Gly Gly Tyr 
            260                 265                 270         


Ala Phe Lys Tyr Glu Asn Gly Lys Tyr Asp Ile Lys Asp Val Gly Val 
        275                 280                 285             


Asp Asn Ala Gly Ala Lys Ala Gly Leu Thr Phe Leu Val Asp Leu Ile 
    290                 295                 300                 


Lys Asn Lys His Met Asn Ala Asp Thr Asp Tyr Ser Ile Ala Glu Ala 
305                 310                 315                 320 


Ala Phe Asn Lys Gly Glu Thr Ala Met Thr Ile Asn Gly Pro Trp Ala 
                325                 330                 335     


Trp Ser Asn Ile Asp Thr Ser Lys Val Asn Tyr Gly Val Thr Val Leu 
            340                 345                 350         


Pro Thr Phe Lys Gly Gln Pro Ser Lys Pro Phe Val Gly Val Leu Ser 
        355                 360                 365             


Ala Gly Ile Asn Ala Ala Ser Pro Asn Lys Glu Leu Ala Lys Glu Phe 
    370                 375                 380                 


Leu Glu Asn Tyr Leu Leu Thr Asp Glu Gly Leu Glu Ala Val Asn Lys 
385                 390                 395                 400 


Asp Lys Pro Leu Gly Ala Val Ala Leu Lys Ser Tyr Glu Glu Glu Leu 
                405                 410                 415     


Ala Lys Asp Pro Arg Ile Ala Ala Thr Met Glu Asn Ala Gln Lys Gly 
            420                 425                 430         


Glu Ile Met Pro Asn Ile Pro Gln Met Ser Ala Phe Trp Tyr Ala Val 
        435                 440                 445             


Arg Thr Ala Val Ile Asn Ala Ala Ser Gly Arg Gln Thr Val Asp Glu 
    450                 455                 460                 


Ala Leu Lys Asp Ala Gln Thr Asn Gly Thr Gly Cys 
465                 470                 475     


<210>  25
<211>  476
<212>  PRT
<213>  Artificial

<220>
<223>  His14-ssNEDD8-MBP

<400>  25

Met Ser Lys His His His His Ser Gly His His His Thr Gly His His 
1               5                   10                  15      


His His Ser Gly Ser His His His Ser Gly Met Leu Ile Lys Val Lys 
            20                  25                  30          


Thr Leu Thr Gly Lys Glu Ile Glu Ile Asp Ile Glu Pro Thr Asp Lys 
        35                  40                  45              


Val Glu Arg Ile Lys Glu Arg Val Glu Glu Lys Glu Gly Ile Pro Pro 
    50                  55                  60                  


Gln Gln Gln Arg Leu Ile Tyr Ser Gly Lys Gln Met Asn Asp Glu Lys 
65                  70                  75                  80  


Thr Ala Ala Asp Tyr Lys Ile Gln Gly Gly Ser Val Leu His Leu Val 
                85                  90                  95      


Leu Ala Leu Arg Gly Gly Ala Gly Thr Lys Thr Glu Glu Gly Lys Leu 
            100                 105                 110         


Val Ile Trp Ile Asn Gly Asp Lys Gly Tyr Asn Gly Leu Ala Glu Val 
        115                 120                 125             


Gly Lys Lys Phe Glu Lys Asp Thr Gly Ile Lys Val Thr Val Glu His 
    130                 135                 140                 


Pro Asp Lys Leu Glu Glu Lys Phe Pro Gln Val Ala Ala Thr Gly Asp 
145                 150                 155                 160 


Gly Pro Asp Ile Ile Phe Trp Ala His Asp Arg Phe Gly Gly Tyr Ala 
                165                 170                 175     


Gln Ser Gly Leu Leu Ala Glu Ile Thr Pro Asp Lys Ala Phe Gln Asp 
            180                 185                 190         


Lys Leu Tyr Pro Phe Thr Trp Asp Ala Val Arg Tyr Asn Gly Lys Leu 
        195                 200                 205             


Ile Ala Tyr Pro Ile Ala Val Glu Ala Leu Ser Leu Ile Tyr Asn Lys 
    210                 215                 220                 


Asp Leu Leu Pro Asn Pro Pro Lys Thr Trp Glu Glu Ile Pro Ala Leu 
225                 230                 235                 240 


Asp Lys Glu Leu Lys Ala Lys Gly Lys Ser Ala Leu Met Phe Asn Leu 
                245                 250                 255     


Gln Glu Pro Tyr Phe Thr Trp Pro Leu Ile Ala Ala Asp Gly Gly Tyr 
            260                 265                 270         


Ala Phe Lys Tyr Glu Asn Gly Lys Tyr Asp Ile Lys Asp Val Gly Val 
        275                 280                 285             


Asp Asn Ala Gly Ala Lys Ala Gly Leu Thr Phe Leu Val Asp Leu Ile 
    290                 295                 300                 


Lys Asn Lys His Met Asn Ala Asp Thr Asp Tyr Ser Ile Ala Glu Ala 
305                 310                 315                 320 


Ala Phe Asn Lys Gly Glu Thr Ala Met Thr Ile Asn Gly Pro Trp Ala 
                325                 330                 335     


Trp Ser Asn Ile Asp Thr Ser Lys Val Asn Tyr Gly Val Thr Val Leu 
            340                 345                 350         


Pro Thr Phe Lys Gly Gln Pro Ser Lys Pro Phe Val Gly Val Leu Ser 
        355                 360                 365             


Ala Gly Ile Asn Ala Ala Ser Pro Asn Lys Glu Leu Ala Lys Glu Phe 
    370                 375                 380                 


Leu Glu Asn Tyr Leu Leu Thr Asp Glu Gly Leu Glu Ala Val Asn Lys 
385                 390                 395                 400 


Asp Lys Pro Leu Gly Ala Val Ala Leu Lys Ser Tyr Glu Glu Glu Leu 
                405                 410                 415     


Ala Lys Asp Pro Arg Ile Ala Ala Thr Met Glu Asn Ala Gln Lys Gly 
            420                 425                 430         


Glu Ile Met Pro Asn Ile Pro Gln Met Ser Ala Phe Trp Tyr Ala Val 
        435                 440                 445             


Arg Thr Ala Val Ile Asn Ala Ala Ser Gly Arg Gln Thr Val Asp Glu 
    450                 455                 460                 


Ala Leu Lys Asp Ala Gln Thr Asn Gly Thr Gly Cys 
465                 470                 475     


<210>  26
<211>  523
<212>  PRT
<213>  Artificial

<220>
<223>  His14-scAtg8-MBP

<400>  26

Met Ser Lys His His His His Ser Gly His His His Thr Gly His His 
1               5                   10                  15      


His His Ser Gly Ser His His His Thr Gly Gly Ser Ser Gly Ser Glu 
            20                  25                  30          


Ser Ser Lys Ser Thr Phe Lys Ser Glu Tyr Pro Phe Glu Lys Arg Lys 
        35                  40                  45              


Ala Glu Ser Glu Arg Ile Ala Asp Arg Phe Lys Asn Arg Ile Pro Val 
    50                  55                  60                  


Ile Cys Glu Lys Ala Glu Lys Ser Asp Ile Pro Glu Ile Asp Lys Arg 
65                  70                  75                  80  


Lys Tyr Leu Val Pro Ala Asp Leu Thr Val Gly Gln Phe Val Tyr Val 
                85                  90                  95      


Ile Arg Lys Arg Ile Met Leu Pro Pro Glu Lys Ala Ile Phe Ile Phe 
            100                 105                 110         


Val Asn Asp Thr Leu Pro Pro Thr Ala Ala Leu Met Ser Ala Ile Tyr 
        115                 120                 125             


Gln Glu His Lys Asp Lys Asp Gly Phe Leu Tyr Val Thr Tyr Ser Gly 
    130                 135                 140                 


Glu Asn Thr Phe Gly Ala Gly Thr Lys Thr Glu Glu Gly Lys Leu Val 
145                 150                 155                 160 


Ile Trp Ile Asn Gly Asp Lys Gly Tyr Asn Gly Leu Ala Glu Val Gly 
                165                 170                 175     


Lys Lys Phe Glu Lys Asp Thr Gly Ile Lys Val Thr Val Glu His Pro 
            180                 185                 190         


Asp Lys Leu Glu Glu Lys Phe Pro Gln Val Ala Ala Thr Gly Asp Gly 
        195                 200                 205             


Pro Asp Ile Ile Phe Trp Ala His Asp Arg Phe Gly Gly Tyr Ala Gln 
    210                 215                 220                 


Ser Gly Leu Leu Ala Glu Ile Thr Pro Asp Lys Ala Phe Gln Asp Lys 
225                 230                 235                 240 


Leu Tyr Pro Phe Thr Trp Asp Ala Val Arg Tyr Asn Gly Lys Leu Ile 
                245                 250                 255     


Ala Tyr Pro Ile Ala Val Glu Ala Leu Ser Leu Ile Tyr Asn Lys Asp 
            260                 265                 270         


Leu Leu Pro Asn Pro Pro Lys Thr Trp Glu Glu Ile Pro Ala Leu Asp 
        275                 280                 285             


Lys Glu Leu Lys Ala Lys Gly Lys Ser Ala Leu Met Phe Asn Leu Gln 
    290                 295                 300                 


Glu Pro Tyr Phe Thr Trp Pro Leu Ile Ala Ala Asp Gly Gly Tyr Ala 
305                 310                 315                 320 


Phe Lys Tyr Glu Asn Gly Lys Tyr Asp Ile Lys Asp Val Gly Val Asp 
                325                 330                 335     


Asn Ala Gly Ala Lys Ala Gly Leu Thr Phe Leu Val Asp Leu Ile Lys 
            340                 345                 350         


Asn Lys His Met Asn Ala Asp Thr Asp Tyr Ser Ile Ala Glu Ala Ala 
        355                 360                 365             


Phe Asn Lys Gly Glu Thr Ala Met Thr Ile Asn Gly Pro Trp Ala Trp 
    370                 375                 380                 


Ser Asn Ile Asp Thr Ser Lys Val Asn Tyr Gly Val Thr Val Leu Pro 
385                 390                 395                 400 


Thr Phe Lys Gly Gln Pro Ser Lys Pro Phe Val Gly Val Leu Ser Ala 
                405                 410                 415     


Gly Ile Asn Ala Ala Ser Pro Asn Lys Glu Leu Ala Lys Glu Phe Leu 
            420                 425                 430         


Glu Asn Tyr Leu Leu Thr Asp Glu Gly Leu Glu Ala Val Asn Lys Asp 
        435                 440                 445             


Lys Pro Leu Gly Ala Val Ala Leu Lys Ser Tyr Glu Glu Glu Leu Ala 
    450                 455                 460                 


Lys Asp Pro Arg Ile Ala Ala Thr Met Glu Asn Ala Gln Lys Gly Glu 
465                 470                 475                 480 


Ile Met Pro Asn Ile Pro Gln Met Ser Ala Phe Trp Tyr Ala Val Arg 
                485                 490                 495     


Thr Ala Val Ile Asn Ala Ala Ser Gly Arg Gln Thr Val Asp Glu Ala 
            500                 505                 510         


Leu Lys Asp Ala Gln Thr Asn Gly Thr Gly Cys 
        515                 520             


<210>  27
<211>  484
<212>  PRT
<213>  Artificial

<220>
<223>  His14-xlUb-MBP

<400>  27

Met Ser Lys His His His His Ser Gly His His His Thr Gly His His 
1               5                   10                  15      


His His Ser Gly Ser His His His Thr Gly Gly Ser Ser Gly Ser Glu 
            20                  25                  30          


Ser Ser Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr 
        35                  40                  45              


Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile 
    50                  55                  60                  


Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala 
65                  70                  75                  80  


Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln 
                85                  90                  95      


Lys Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly Ala Gly 
            100                 105                 110         


Thr Lys Thr Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys 
        115                 120                 125             


Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr 
    130                 135                 140                 


Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe 
145                 150                 155                 160 


Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala 
                165                 170                 175     


His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile 
            180                 185                 190         


Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp 
        195                 200                 205             


Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu 
    210                 215                 220                 


Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys 
225                 230                 235                 240 


Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly 
                245                 250                 255     


Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 
            260                 265                 270         


Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys 
        275                 280                 285             


Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly 
    290                 295                 300                 


Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp 
305                 310                 315                 320 


Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala 
                325                 330                 335     


Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 
            340                 345                 350         


Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser 
        355                 360                 365             


Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro 
    370                 375                 380                 


Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp 
385                 390                 395                 400 


Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala 
                405                 410                 415     


Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala 
            420                 425                 430         


Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln 
        435                 440                 445             


Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala 
    450                 455                 460                 


Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn 
465                 470                 475                 480 


Gly Thr Gly Cys 
                


<210>  28
<211>  497
<212>  PRT
<213>  Artificial

<220>
<223>  His14-scSUMO-MBP

<400>  28

Met Ser Lys His His His His Ser Gly His His His Thr Gly His His 
1               5                   10                  15      


His His Ser Gly Ser His His His Thr Gly Ser Asp Ser Glu Val Asn 
            20                  25                  30          


Gln Glu Ala Lys Pro Glu Val Lys Pro Glu Val Lys Pro Glu Thr His 
        35                  40                  45              


Ile Asn Leu Lys Val Ser Asp Gly Ser Ser Glu Ile Phe Phe Lys Ile 
    50                  55                  60                  


Lys Lys Thr Thr Pro Leu Arg Arg Leu Met Glu Ala Phe Ala Lys Arg 
65                  70                  75                  80  


Gln Gly Lys Glu Met Asp Ser Leu Arg Phe Leu Tyr Asp Gly Ile Arg 
                85                  90                  95      


Ile Gln Ala Asp Gln Thr Pro Glu Asp Leu Asp Met Glu Asp Asn Asp 
            100                 105                 110         


Ile Ile Glu Ala His Arg Glu Gln Ile Gly Gly Ala Gly Thr Lys Thr 
        115                 120                 125             


Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys Gly Tyr Asn 
    130                 135                 140                 


Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr Gly Ile Lys 
145                 150                 155                 160 


Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe Pro Gln Val 
                165                 170                 175     


Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala His Asp Arg 
            180                 185                 190         


Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile Thr Pro Asp 
        195                 200                 205             


Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp Ala Val Arg 
    210                 215                 220                 


Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu Ala Leu Ser 
225                 230                 235                 240 


Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys Thr Trp Glu 
                245                 250                 255     


Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly Lys Ser Ala 
            260                 265                 270         


Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro Leu Ile Ala 
        275                 280                 285             


Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys Tyr Asp Ile 
    290                 295                 300                 


Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly Leu Thr Phe 
305                 310                 315                 320 


Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp Thr Asp Tyr 
                325                 330                 335     


Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala Met Thr Ile 
            340                 345                 350         


Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys Val Asn Tyr 
        355                 360                 365             


Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser Lys Pro Phe 
    370                 375                 380                 


Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro Asn Lys Glu 
385                 390                 395                 400 


Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp Glu Gly Leu 
                405                 410                 415     


Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala Leu Lys Ser 
            420                 425                 430         


Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala Thr Met Glu 
        435                 440                 445             


Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln Met Ser Ala 
    450                 455                 460                 


Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala Ser Gly Arg 
465                 470                 475                 480 


Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn Gly Thr Gly 
                485                 490                 495     


Cys 
    


