
                                SEQUENCE LISTING

<110> Regents of the University of Minnesota

<120> CONFERRING RESISTANCE TO GEMINIVIRUSES
  IN PLANTS USING CRISPR/CAS SYSTEMS
  

<130> 09531-0341WO1

<150> 61/884,236          
<151> 2013-09-30  

<160> 55

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 1379
<212> PRT
<213> Streptococcus pyogenese

<400> 1
Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val
 1               5                  10                  15      
Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser
                85                  90                  95      
Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys
            100                 105                 110         
His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr
        115                 120                 125             
His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp
    130                 135                 140                 
Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro
                165                 170                 175     
Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr
            180                 185                 190         
Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala
        195                 200                 205             
Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn
    210                 215                 220                 
Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe
                245                 250                 255     
Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp
            260                 265                 270         
Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp
    290                 295                 300                 
Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser
305                 310                 315                 320 
Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys
                325                 330                 335     
Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe
            340                 345                 350         
Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser
        355                 360                 365             
Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp
    370                 375                 380                 
Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu
                405                 410                 415     
Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe
            420                 425                 430         
Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp
    450                 455                 460                 
Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu
465                 470                 475                 480 
Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr
                485                 490                 495     
Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser
            500                 505                 510         
Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys
        515                 520                 525             
Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln
    530                 535                 540                 
Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr
545                 550                 555                 560 
Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp
                565                 570                 575     
Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp
        595                 600                 605             
Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala
625                 630                 635                 640 
His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe
    690                 695                 700                 
Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu
705                 710                 715                 720 
His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly
                725                 730                 735     
Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly
            740                 745                 750         
Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln
        755                 760                 765             
Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile
    770                 775                 780                 
Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro
785                 790                 795                 800 
Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu
                805                 810                 815     
Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg
            820                 825                 830         
Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys
        835                 840                 845             
Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys
865                 870                 875                 880 
Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp
    930                 935                 940                 
Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser
945                 950                 955                 960 
Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg
                965                 970                 975     
Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val
            980                 985                 990         
Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu Glu Ser Glu Phe
        995                 1000                1005            
Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys Met Ile Ala Lys
    1010                1015                1020                
Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser
1025                1030                1035               1040 
Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu Ala Asn Gly Glu
                1045                1050                1055    
Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu Thr Gly Glu Ile
            1060                1065                1070        
Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg Lys Val Leu Ser
        1075                1080                1085            
Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser Asp Lys Leu Ile
1105                1110                1115               1120 
Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu Lys Gly
            1140                1145                1150        
Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu Gly Ile Thr Ile
        1155                1160                1165            
Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp Phe Leu Glu Ala
    1170                1175                1180                
Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile Lys Leu Pro Lys
1185                1190                1195               1200 
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg Met Leu Ala Ser
                1205                1210                1215    
Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu Pro Ser Lys Tyr
            1220                1225                1230        
Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys Leu Lys Gly Ser
        1235                1240                1245            
Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu Gln His Lys His
    1250                1255                1260                
Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe Ser Lys Arg Val
1265                1270                1275               1280 
Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser Ala Tyr Asn Lys
                1285                1290                1295    
His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn Ile Ile His Leu
            1300                1305                1310        
Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe Lys Tyr Phe Asp
        1315                1320                1325            
Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu Asp
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile
1345                1350                1355               1360 
Asp Leu Ser Gln Leu Gly Gly Asp Ser Arg Ala Asp Pro Lys Lys Lys
                1365                1370                1375    
Arg Lys Val
            


<210> 2
<211> 13
<212> DNA
<213> tomato golden mosaic virus

<400> 2
ggtagtaagg tag                                                    13

<210> 3
<211> 16
<212> DNA
<213> mild strain of bean yellow dwarf virus

<400> 3
tggaggcatg gaggca                                                 16

<210> 4
<211> 12
<212> DNA
<213> mild strain of bean yellow dwarf virus

<400> 4
ttccttacct at                                                     12

<210> 5
<211> 21
<212> DNA
<213> mild strain of bean yellow dwarf virus

<400> 5
cactatcatg ctcttctcca g                                           21

<210> 6
<211> 21
<212> DNA
<213> mild strain of bean yellow dwarf virus

<400> 6
gtccttgatt acatatcaaa g                                           21

<210> 7
<211> 15
<212> DNA
<213> mild strain of bean yellow dwarf virus

<400> 7
cttcgctgcc acgaa                                                  15

<210> 8
<211> 23
<212> DNA
<213> mild strain of bean yellow dwarf virus

<400> 8
gcgacaaggg ggggcccacg ccg                                         23

<210> 9
<211> 26
<212> DNA
<213> cabbage leaf curl virus

<400> 9
atgtatccta caaagtttag gcgtgg                                      26

<210> 10
<211> 24
<212> DNA
<213> cabbage leaf curl virus

<400> 10
ctatgtaatt aaacgcattt ggag                                        24

<210> 11
<211> 12
<212> DNA
<213> mild strain of bean yellow dwarf virus

<400> 11
ataggtaagg aa                                                     12

<210> 12
<211> 21
<212> DNA
<213> mild strain of bean yellow dwarf virus

<400> 12
ctggagaaga gcatgatagt g                                           21

<210> 13
<211> 21
<212> DNA
<213> mild strain of bean yellow dwarf virus

<400> 13
ctttgatatg taatcaagga c                                           21

<210> 14
<211> 15
<212> DNA
<213> mild strain of bean yellow dwarf virus

<400> 14
ttcgtggcag cgaag                                                  15

<210> 15
<211> 20
<212> DNA
<213> cabbage leaf curl virus

<400> 15
ctatgtaatt aaacgcattt                                             20

<210> 16
<211> 13
<212> DNA
<213> tomato golden mosaic virus

<400> 16
ctaccttact acc                                                    13

<210> 17
<211> 16
<212> DNA
<213> mild strain of bean yellow dwarf virus

<400> 17
tgcctccatg cctcca                                                 16

<210> 18
<211> 23
<212> DNA
<213> mild strain of bean yellow dwarf virus

<400> 18
cggcgtgggc ccccccttgt cgc                                         23

<210> 19
<211> 26
<212> DNA
<213> cabbage leaf curl virus

<400> 19
ccacgcctaa actttgtagg atacat                                      26

<210> 20
<211> 20
<212> DNA
<213> cabbage leaf curl virus

<400> 20
aaatgcgttt aattacatag                                             20

<210> 21
<211> 21
<212> DNA
<213> tomato golden mosaic virus

<400> 21
tttcttacat atcctcagtg c                                           21

<210> 22
<211> 21
<212> DNA
<213> tomato golden mosaic virus

<400> 22
cacctccacg tgcttattca g                                           21

<210> 23
<211> 21
<212> DNA
<213> tomato golden mosaic virus

<400> 23
gtcaagacgt acatcgacaa a                                           21

<210> 24
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic oligonucleotide

<400> 24
ctccgaattc gcccttcacc atggattaca aggatgatga tg                    42

<210> 25
<211> 44
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic oligonucleotide

<400> 25
aaatgtttga acgatcggac gtctcacttc ttcttcttag cctg                  44

<210> 26
<211> 104
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic oligonucleotide

<220> 
<221> misc_feature   
<222> 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18,
19, 20, 21
<223> n = A,T,C or G

<400> 26
gnnnnnnnnn nnnnnnnnnn ngttttagag ctagaaatag caagttaaaa taaggctagt 60
ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttt                  104

<210> 27
<211> 37
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic oligonucleotide

<400> 27
tcgtctcgtc gaccggccgc gatgttgttg ttaccag                          37

<210> 28
<211> 31
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic oligonucleotide

<400> 28
gactcactat aggggatatc agctggatgg c                                31

<210> 29
<211> 20
<212> DNA
<213> bean yellow dwarf virus

<400> 29
gcgtggaggc atggaggcag                                             20

<210> 30
<211> 20
<212> DNA
<213> bean yellow dwarf virus

<400> 30
ctgcctccat gcctccacgc                                             20

<210> 31
<211> 20
<212> DNA
<213> bean yellow dwarf virus

<400> 31
cacgccgaat ttaatattac                                             20

<210> 32
<211> 20
<212> DNA
<213> bean yellow dwarf virus

<400> 32
ggtaatatta aattcggcgt                                             20

<210> 33
<211> 20
<212> DNA
<213> bean yellow dwarf virus

<400> 33
gaagtctttg cgacaagggg                                             20

<210> 34
<211> 20
<212> DNA
<213> bean yellow dwarf virus

<400> 34
aaagcactcg cgataagggg                                             20

<210> 35
<211> 20
<212> DNA
<213> bean yellow dwarf virus

<400> 35
cgcgagtgct ttagcacgag                                             20

<210> 36
<211> 20
<212> DNA
<213> bean yellow dwarf virus

<400> 36
atgagcactt gggataggta                                             20

<210> 37
<211> 20
<212> DNA
<213> bean yellow dwarf virus

<400> 37
gctggagaag agcatgatag                                             20

<210> 38
<211> 20
<212> DNA
<213> bean yellow dwarf virus

<400> 38
cgtcctttga tatgtaatca                                             20

<210> 39
<211> 20
<212> DNA
<213> bean yellow dwarf virus

<400> 39
cttgattaca tatcaaagga                                             20

<210> 40
<211> 20
<212> DNA
<213> tomato golden mosaic virus

<400> 40
agttatatga attggtagta                                             20

<210> 41
<211> 20
<212> DNA
<213> tomato golden mosaic virus

<400> 41
ctaccaattc atataacttt                                             20

<210> 42
<211> 20
<212> DNA
<213> tomato golden mosaic virus

<400> 42
ggccatccgt ttaatattac                                             20

<210> 43
<211> 20
<212> DNA
<213> tomato golden mosaic virus

<400> 43
ggccatccgg taatattaaa                                             20

<210> 44
<211> 20
<212> DNA
<213> tomato golden mosaic virus

<400> 44
atccgtttaa tattaccgga                                             20

<210> 45
<211> 20
<212> DNA
<213> tomato golden mosaic virus

<400> 45
atccggtaat attaaacgga                                             20

<210> 46
<211> 20
<212> DNA
<213> tomato golden mosaic virus

<400> 46
tctttggaca aggagcactg                                             20

<210> 47
<211> 20
<212> DNA
<213> tomato golden mosaic virus

<400> 47
cgaactgaat aagcacgtgg                                             20

<210> 48
<211> 20
<212> DNA
<213> tomato golden mosaic virus

<400> 48
ccacgtgctt attcagttcg                                             20

<210> 49
<211> 20
<212> DNA
<213> tomato golden mosaic virus

<400> 49
gtcgatgtac gtcttgacgt                                             20

<210> 50
<211> 20
<212> DNA
<213> tomato golden mosaic virus

<400> 50
aagacgtaca tcgacaaaga                                             20

<210> 51
<211> 20
<212> DNA
<213> tomato yellow leaf curl virus

<400> 51
ccgattgcca tagagctttg                                             20

<210> 52
<211> 20
<212> DNA
<213> tomato yellow leaf curl virus

<400> 52
caattgttct ctctctaaag                                             20

<210> 53
<211> 20
<212> DNA
<213> tomato yellow leaf curl virus

<400> 53
catgtgctta tccaattcga                                             20

<210> 54
<211> 20
<212> DNA
<213> tomato yellow leaf curl virus

<400> 54
aacagatgtc aagacctacg                                             20

<210> 55
<211> 1366
<212> DNA
<213> cotton leaf curl virus

<400> 55
accgtgggcg agcggtgtct ttggcgttcc atgtgggtcc cacaatatcc aaaagaagaa 60
taatggactg ggtcaatgca attgggcctt aaatgaaatg ggcttggacc agtagattcg 120
agactgggcc aatagaataa aacaacaaat ggactcataa tcaaaacaaa gtgtttattc 180
atgtcaaata cattacacac tcacacacac acagtcgtac acacatcata ttcatcccct 240
atacgtatat caactaatgg ggcctcatgc atcatcatta tatcaatagc ctctaccatg 300
tcctcctggc gaaagtcccg aacatgacaa tccctataca tgatctttaa tatattatgt 360
atcccttcct ccaaattgtt gaagtcgaaa ggcggtatga tcccatcatg gccgtatggg 420
atcatgaagg tcttctttgc cagggcaggt gatcttgttg agcacaattc aatccgcaca 480
aggattgaat tgtcctcgtg gatcttcacg tcgacggtaa ataccatccc cttctcgtta 540
gtatacttga ttgtcatttg catttaatta tgaacaacac atgagatgaa tcttcttaaa 600
tagcgtccat atatttggat atatggacat aatgcatata cgtggtgcaa taattatcat 660
atgaatatga gtggagacat atatgattga tatctacagg actatcaatc atcgtcaaga 720
aaaaagggat aaaagaaacc ttattcatga aaggattagg tagggaaaaa taaaagaagg 780
aaattaagga aaagaaaacc cacaatgaaa taattaagaa aaaaaaaaga aaaaaagaac 840
acaagaaagg aaaacagaaa catgagcaca aacagaaacc tcttgagaaa atattggagc 900
gcagcgggaa aaccaagaaa aacaaaccaa ggaagacact tgagcaaaaa gggaaaacac 960
aaactaaact aggagggtcc aacatgaaat tatgaaaagt ccgtacacag taattaatca 1020
ttaattactg cgcagtaaat gtgaataaaa ttaacccgaa gggttaattt tcgagacccc 1080
gataggtaat tgagtcccca atatatcggg gactcaatcg gggtcatgag agagaaataa 1140
atcccggata ccgaaactac cctcaaagct gtgtctggaa ggcgcgtggg agtgcgctga 1200
aaaaggtgac cttctctctc caaaaactca ccggaacggc caaactggct gattccggca 1260
tctaatcacg acacgcgcgg cggtgtgtac ccctgggagg gtagaccact acgctacgca 1320
gcagccttag ctacgccgga gcttagctcg cccacgttct aatatt                1366

