                         SEQUENCE LISTING

<110>  University of Florida Research Foundation, Inc.
 
<120>  METHODS AND ENZYMES FOR PRODUCING HYDROXYMETHYLFURFURAL

<130>  222108-2820

<150>  US 61/882,682
<151>  2013-09-26

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  432
<212>  PRT
<213>  Thermotoga martitima

 

<400>  1

Met Phe Lys Pro Asn Tyr His Phe Phe Pro Ile Thr Gly Trp Met Asn 
1               5                   10                  15      


Asp Pro Asn Gly Leu Ile Phe Trp Lys Gly Lys Tyr His Met Phe Tyr 
            20                  25                  30          


Gln Tyr Asn Pro Arg Lys Pro Glu Trp Gly Asn Ile Cys Trp Gly His 
        35                  40                  45              


Ala Val Ser Asp Asp Leu Val His Trp Arg His Leu Pro Val Ala Leu 
    50                  55                  60                  


Tyr Pro Asp Asp Glu Thr His Gly Val Phe Ser Gly Ser Ala Val Glu 
65                  70                  75                  80  


Lys Asp Gly Lys Met Phe Leu Val Tyr Thr Tyr Tyr Arg Asp Pro Thr 
                85                  90                  95      


His Asn Lys Gly Glu Lys Glu Thr Gln Cys Val Ala Met Ser Glu Asn 
            100                 105                 110         


Gly Leu Asp Phe Val Lys Tyr Asp Gly Asn Pro Val Ile Ser Lys Pro 
        115                 120                 125             


Pro Glu Glu Gly Thr His Ala Phe Arg Asp Pro Lys Val Asn Arg Ser 
    130                 135                 140                 


Asn Gly Glu Trp Arg Met Val Leu Gly Ser Gly Lys Asp Glu Lys Ile 
145                 150                 155                 160 


Gly Arg Val Leu Leu Tyr Thr Ser Asp Asp Leu Phe His Trp Lys Tyr 
                165                 170                 175     


Glu Gly Val Ile Phe Glu Asp Glu Thr Thr Lys Glu Ile Glu Cys Pro 
            180                 185                 190         


Asp Leu Val Arg Ile Gly Glu Lys Asp Ile Leu Ile Tyr Ser Ile Thr 
        195                 200                 205             


Ser Thr Asn Ser Val Leu Phe Ser Met Gly Glu Leu Lys Glu Gly Lys 
    210                 215                 220                 


Leu Asn Val Glu Lys Arg Gly Leu Leu Asp His Gly Thr Asp Phe Tyr 
225                 230                 235                 240 


Ala Ala Gln Thr Phe Phe Gly Thr Asp Arg Val Val Val Ile Gly Trp 
                245                 250                 255     


Leu Gln Ser Trp Leu Arg Thr Gly Leu Tyr Pro Thr Lys Arg Glu Gly 
            260                 265                 270         


Trp Asn Gly Val Met Ser Leu Pro Arg Glu Leu Tyr Val Glu Asn Asn 
        275                 280                 285             


Glu Leu Lys Val Lys Pro Val Asp Glu Leu Leu Ala Leu Arg Lys Arg 
    290                 295                 300                 


Lys Val Phe Glu Thr Ala Lys Ser Gly Thr Phe Leu Leu Asp Val Lys 
305                 310                 315                 320 


Glu Asn Ser Tyr Glu Ile Val Cys Glu Phe Ser Gly Glu Ile Glu Leu 
                325                 330                 335     


Arg Met Gly Asn Glu Ser Glu Glu Val Val Ile Thr Lys Ser Arg Asp 
            340                 345                 350         


Glu Leu Ile Val Asp Thr Thr Arg Ser Gly Val Ser Gly Gly Glu Val 
        355                 360                 365             


Arg Lys Ser Thr Val Glu Asp Glu Ala Thr Asn Arg Ile Arg Ala Phe 
    370                 375                 380                 


Leu Asp Ser Cys Ser Val Glu Phe Phe Phe Asn Asp Ser Ile Ala Phe 
385                 390                 395                 400 


Ser Phe Arg Ile His Pro Glu Asn Val Tyr Asn Ile Leu Ser Val Lys 
                405                 410                 415     


Ser Asn Gln Val Lys Leu Glu Val Phe Glu Leu Glu Asn Ile Trp Leu 
            420                 425                 430         


<210>  2
<211>  1299
<212>  DNA
<213>  Thermotoga martitima


<400>  2

atgttcaagc cgaattatca ctttttcccg ataacaggct ggatgaacga tccgaacggt       60

ttgatcttct ggaagggaaa atatcatatg ttctatcagt ataatcccag aaaacctgag      120

tggggaaaca tctgctgggg ccacgcggtg agcgacgatc tcgttcactg gagacacctt      180

cccgttgctc tatatcccga cgatgaaaca cacggagtgt tctctggaag cgctgtcgag      240

aaagatggga aaatgtttct cgtgtacacc tactaccgcg atccgacaca caacaaagga      300

gaaaaagaaa cccagtgtgt ggctatgagt gaaaacggat tggatttcgt aaagtacgat      360

ggaaacccgg tcatatctaa acccccagag gaagggacgc acgccttcag agacccgaag      420

gtgaacagaa gcaacggtga gtggcgaatg gtactgggat ctggtaaaga tgagaagatt      480

ggaagagtgc ttctctatac ctcagatgac ctttttcact ggaagtacga gggtgtgatc      540

ttcgaagatg aaaccacaaa agaaatagag tgtcccgatc ttgtgagaat tggagagaaa      600

gatatcctca tatactcgat aacgagtaca aacagcgttc tgttttccat gggagagtta      660

aaggaaggaa aactgaatgt cgaaaagcgg gggcttctcg atcacggaac ggatttctac      720

gctgctcaaa ctttctttgg aacagacaga gttgtagtta tcggatggct tcaaagctgg      780

ttgagaacag ggctttaccc gacaaaacga gaaggatgga acggtgtcat gagtcttcct      840

agggagctgt atgtagaaaa caacgagttg aaggtgaaac cggtggatga actcttggct      900

ctcagaaaga gaaaggtttt cgaaactgca aagtccggaa catttctgct ggatgtcaag      960

gaaaacagtt atgaaattgt gtgtgaattc agcggagaaa tcgaacttcg aatgggaaat     1020

gaatctgaag aagtggtgat aacgaagagt cgagacgaat taatcgtgga tacaacgaga     1080

tctggtgttt caggtggaga agttagaaag tcgacagtcg aagatgaagc tacaaataga     1140

atacgagctt tcttggattc gtgttctgta gaatttttct tcaacgactc catagctttt     1200

tcctttagga tccatccaga gaacgtttac aacattcttt ctgtcaaatc gaaccaagtg     1260

aaactcgaag tctttgaact cgagaacata tggttgtga                            1299


<210>  3
<211>  1299
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically synthesized codon optimized nucleotide
       sequence of Beta-fructofuranosidase

<400>  3

atgttcaagc cgaattatca ctttttcccg ataaccggtt ggatgaacga tccgaacggt       60

ttgatcttct ggaagggaaa atatcacatg ttctatcagt ataatcccag aaaacctgag      120

tggggaaaca tctgctgggg ccacgcggtg agcgacgatc tcgttcactg gagacacctt      180

cccgttgctc tatatcccga cgatgaaaca cacggagtgt tctctggaag cgctgtcgag      240

aaagatggga aaatgtttct cgtgtacacc tactaccgcg atccgacaca caacaaagga      300

gaaaaagaaa cccagtgtgt ggctatgagt gaaaacggat tggatttcgt aaagtacgat      360

ggaaacccgg tcatatctaa acccccagag gaagggacgc acgccttcag agacccgaag      420

gtgaacagaa gcaacggtga gtggcgaatg gtactgggat ctggtaaaga tgagaagatt      480

ggaagagtgc ttctctatac ctcagatgac ctttttcact ggaagtacga gggtgtgatc      540

ttcgaagatg aaaccacaaa agaaatagag tgtcccgatc ttgtgagaat tggagagaaa      600

gatatcctca tatactcgat aacgagtaca aacagcgttc tgttttccat gggagagtta      660

aaggaaggaa aactgaatgt cgaaaagcgg gggcttctcg atcacggaac ggatttctac      720

gctgctcaaa ctttctttgg taccgacaga gttgtagtta tcggatggct tcaaagctgg      780

ttgagaacag ggctttaccc gacaaaacga gaaggatgga acggtgtcat gagtcttcct      840

agggagctgt atgtagaaaa caacgagttg aaggtgaaac ctgtggatga actcttggct      900

ctcagaaaga gaaaggtttt cgaaactgca aagtccggaa catttctgct ggatgtcaag      960

gaaaacagtt atgaaattgt gtgtgaattc agcggagaaa tcgaacttcg aatgggaaat     1020

gaatctgaag aagtggtgat aacgaagagt cgagacgaat taatcgtgga tacaacgaga     1080

tctggtgttt caggtggaga agttagaaag tcgacagtcg aagatgaagc tacaaataga     1140

atacgagctt tcttggattc gtgttctgta gaatttttct tcaacgactc catagctttt     1200

tcctttagga tccatccaga gaacgtttac aacattcttt ctgtcaaatc gaaccaagtg     1260

aaactcgaag tctttgaact cgagaatatt tggttgtga                            1299


