                         SEQUENCE LISTING

<110>  The Board of Trustees of the University of Illinois
       Kierny, Michael
       Kay, Brian
 
<120>  Method and Kit for Generating High Affinity Binding Agents

<130>  UIC0042WO

<150>  US 61/883,547
<151>  2013-09-27

<150>  US 61/988,559
<151>  2014-05-05

<150>  US 61/991,121
<151>  2014-05-09

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  94
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<400>  1

Val Ser Asp Val Pro Arg Asp Leu Glu Val Val Ala Ala Thr Pro Thr 
1               5                   10                  15      


Ser Leu Leu Ile Ser Trp Asp Ala Pro Ala Val Thr Val Arg Tyr Tyr 
            20                  25                  30          


Arg Ile Thr Tyr Gly Glu Thr Gly Gly Asn Ser Pro Val Gln Glu Phe 
        35                  40                  45              


Thr Val Pro Gly Ser Lys Ser Thr Ala Thr Ile Ser Gly Leu Lys Pro 
    50                  55                  60                  


Gly Val Asp Tyr Thr Ile Thr Val Tyr Ala Val Thr Gly Arg Gly Asp 
65                  70                  75                  80  


Ser Pro Ala Ser Ser Lys Pro Ile Ser Ile Asn Tyr Arg Thr 
                85                  90                  


<210>  2
<211>  94
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<400>  2

Met Ala Val Ser Asp Val Pro Arg Lys Leu Glu Val Val Ala Ala Thr 
1               5                   10                  15      


Pro Thr Ser Leu Leu Ile Ser Trp Asp Ala Pro Cys Arg Lys Cys Leu 
            20                  25                  30          


Tyr Tyr Arg Ile Thr Tyr Gly Glu Thr Gly Gly Asn Ser Pro Val Gln 
        35                  40                  45              


Glu Phe Thr Val Pro Gly Ser Lys Ser Thr Ala Thr Ile Ser Gly Leu 
    50                  55                  60                  


Lys Pro Gly Val Asp Tyr Thr Ile Thr Val Tyr Ala Val Thr Arg Leu 
65                  70                  75                  80  


Glu Phe Ile Ser Lys Pro Ile Ile Ser Ile Asn Tyr Arg Ile 
                85                  90                  


<210>  3
<211>  41
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  3
atacgaaatt aatacgactc actataggga gaccacaacg g                           41


<210>  4
<211>  45
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  4
ccgcacacca gtaaggtgtg cggtttcagt tgccgctttc tttct                       45


<210>  5
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  5

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  6
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  6
gccgctggta cggtagttaa tcgag                                             25


<210>  7
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide

<400>  7
atggccgttt ctgatgttcc gcgta                                             25


<210>  8
<211>  90
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<400>  8

Met Ala Val Ser Asp Val Pro Arg Lys Leu Glu Val Val Ala Ala Thr 
1               5                   10                  15      


Pro Thr Ser Leu Leu Ile Ser Trp Asp Gln Asp Gln Val Tyr Tyr Tyr 
            20                  25                  30          


Arg Ile Arg Tyr Gly Glu Thr Gly Gly Asn Ser Pro Val Gln Glu Phe 
        35                  40                  45              


Thr Val Pro Gly Ser Lys Ser Thr Ala Thr Ile Ser Gly Leu Lys Pro 
    50                  55                  60                  


Gly Val Asp Tyr Thr Ile Thr Val Tyr Ala Val Thr Gly Thr Ser Tyr 
65                  70                  75                  80  


Ala Tyr Ile Ile Ser Ile Asn Tyr Arg Ile 
                85                  90  


<210>  9
<211>  96
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<400>  9

Met Ala Val Ser Asp Val Pro Arg Lys Leu Glu Val Val Ala Ala Thr 
1               5                   10                  15      


Pro Thr Ser Leu Leu Ile Ser Trp Asp Pro Tyr Trp Phe Ser Gly Leu 
            20                  25                  30          


Tyr Tyr Arg Ile Thr Tyr Gly Glu Thr Gly Gly Asn Ser Pro Val Gln 
        35                  40                  45              


Glu Phe Thr Val Pro Gly Ser Lys Ser Thr Ala Thr Ile Ser Gly Leu 
    50                  55                  60                  


Lys Pro Gly Val Asp Tyr Thr Ile Thr Val Tyr Ala Val Thr Tyr Tyr 
65                  70                  75                  80  


Ser Trp Gly Tyr Leu Val Gly Glu Ile Ile Ser Ile Asn Tyr Arg Ile 
                85                  90                  95      


<210>  10
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  10

Lys Lys Phe Glu Leu Leu Pro Thr Pro Pro Leu Ser Pro Ser Tyr 
1               5                   10                  15  


<210>  11
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  PHOSPHORYLATION

<400>  11

Lys Lys Phe Glu Leu Leu Pro Thr Pro Pro Leu Ser Pro Ser Tyr 
1               5                   10                  15  


<210>  12
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  PHOSPHORYLATION

<220>
<221>  MOD_RES
<222>  (12)..(12)
<223>  PHOSPHORYLATION

<400>  12

Lys Lys Phe Glu Leu Leu Pro Thr Pro Pro Leu Ser Pro Ser Tyr 
1               5                   10                  15  


<210>  13
<211>  13
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MOD_RES
<222>  (6)..(6)
<223>  PHOSPHORYLATION

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  PHOSPHORYLATION

<400>  13

His Thr Gly Phe Leu Thr Glu Tyr Val Ala Thr Arg Trp 
1               5                   10              


<210>  14
<211>  17
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  PHOSPHORYLATION

<220>
<221>  MOD_RES
<222>  (12)..(12)
<223>  PHOSPHORYLATION

<400>  14

Glu Ala Arg Ser Arg Asp Ala Thr Pro Pro Val Ser Pro Ile Asn Tyr 
1               5                   10                  15      


Lys 
    


<210>  15
<211>  75
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (29)..(43)
<223>  n encodes a BC loop residue

<220>
<221>  misc_feature
<222>  (44)..(52)
<223>  n is present or absent and when present encode a BC loop residue

<400>  15
cggtttcacc gtacgtgata cggtaatann nnnnnnnnnn nnnnnnnnnn nnatcccagc       60

tgatcagcag gctag                                                        75


<210>  16
<211>  95
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (26)..(46)
<223>  n encodes an FG loop residue

<220>
<221>  misc_feature
<222>  (47)..(64)
<223>  n is present or absent and when present encodes an FG loop 
       residue

<400>  16
cgctggtacg gtagttaatc gagatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       60

nnnnagtaac agcgtataca gtgatggtat agtca                                  95


<210>  17
<211>  68
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (19)..(27)
<223>  n encodes a DE loop residue

<220>
<221>  misc_feature
<222>  (28)..(42)
<223>  n is present or absent and when present encodes a DE loop residue

<400>  17
gccgctgatg gtagcagtnn nnnnnnnnnn nnnnnnnnnn nnatcaggta cagtgaactc       60

ctgaaccg                                                                68


