﻿               SEQUENCE LISTING

<110> Universitat Autònoma de Barcelona

<120> GENE THERAPY COMPOSITIONS FOR USE IN THE PREVENTION AND/OR TREATMENT OF NON-ALCOHOLIC FATTY LIVER DISEASE.

<130> EP-06637

<160> 55

<170> BiSSAP 1.2

<210> 1
<211> 7908
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..7908
<223> /mol_type="unassigned DNA"
      /note="Artificial plasmid pGG3-HCR/hAAT-OptSirt-1"
      /organism="Artificial Sequence"

<220> 
<221> promoter
<222> 4321..5414

<220> 
<221> intron
<222> 5458..5542

<220> 
<221> gene
<222> 5683..7908

<220> 
<221> polyA_site
<222> 31..197

<220> 
<221> misc_feature
<222> 4116..4256
<223> /standard_name="5´Inverted Terminal Repeat (ITR) AAV2 sequence"

<220> 
<221> misc_feature
<222> 356..507
<223> /standard_name="3´Inverted Terminal Repeat (ITR) AAV2 sequence"

<400> 1
tcgagacgcg tgatatcgga tcccggccgg cggccgcttc cctttagtga gggttaatgc       60

ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt      120

gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa      180

gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg      240

agatgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atccgataag      300

ggactagagc atggctacgt agataagtag catggcgggt taatcattaa ctacaaggaa      360

cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg      420

cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg      480

cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag      540

cctgaatggc gaatggaatt ccagacgatt gagcgtcaaa atgtaggtat ttccatgagc      600

gtttttccgt tgcaatggct ggcggtaata ttgttctgga tattaccagc aaggccgata      660

gtttgagttc ttctactcag gcaagtgatg ttattactaa tcaaagaagt attgcgacaa      720

cggttaattt gcgtgatgga cagactcttt tactcggtgg cctcactgat tataaaaaca      780

cttctcagga ttctggcgta ccgttcctgt ctaaaatccc tttaatcggc ctcctgttta      840

gctcccgctc tgattctaac gaggaaagca cgttatacgt gctcgtcaaa gcaaccatag      900

tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc      960

gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc     1020

acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt     1080

agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg     1140

ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt     1200

ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta     1260

taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt     1320

aacgcgaatt ttaacaaaat attaacgtct acaatttaaa tatttgctta tacaatcttc     1380

ctgtttttgg ggcttttctg attatcaacc ggggtacata tgattgacat gctagtttta     1440

cgattaccgt tcatcgattc tcttgtttgc tccagactct caggcaatga cctgatagcc     1500

tttgtagaga cctctcaaaa atagctaccc tctccggcat gaatttatca gctagaacgg     1560

ttgaatatca tattgatggt gatttgactg tctccggcct ttctcacccg tttgaatctt     1620

tacctacaca ttactcaggc attgcattta aaatatatga gggttctaaa aatttttatc     1680

cttgcgttga aataaaggct tctcccgcaa aagtattaca gggtcataat gtttttggta     1740

caaccgattt agctttatgc tctgaggctt tattgcttaa ttttgctaat tctttgcctt     1800

gcctgtatga tttattggat gttggaatcg cctgatgcgg tattttctcc ttacgcatct     1860

gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata     1920

gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct     1980

cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt     2040

ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata     2100

ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt     2160

gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag     2220

acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca     2280

tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc     2340

agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat     2400

cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc     2460

aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg     2520

gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc     2580

agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat     2640

aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga     2700

gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc     2760

ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc     2820

aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt     2880

aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc     2940

tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc     3000

agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca     3060

ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca     3120

ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt     3180

ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta     3240

acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg     3300

agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc     3360

ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag     3420

cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa     3480

gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc     3540

cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc     3600

gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta     3660

caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag     3720

aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct     3780

tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga     3840

gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc     3900

ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt     3960

atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg     4020

cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg     4080

caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcagctgcg cgctcgctcg     4140

ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg cccggcctca     4200

gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg ttccttgtag     4260

ttaatgatta acccgccatg ctacttatct acgtagccat gctctagaca tggctcgaca     4320

gatctgatat catcgatgaa ttcgagctcg gtacccggcc gcagatttag gtgacactat     4380

agaatatgca tcactagtaa gcttgcgaat tccagtctac agagaggtct ctgacctctg     4440

ccccagctcc aaggtcagca ggcagggagg gctgtgtgtt tgctgtttgc tgcttgcaat     4500

gtttgcccat tttagggaca tgagtaggct gaagtttgtt cagtgtggac ttcagaggca     4560

gcacacaaac agcaagcttg cgaattccag tctacagaga ggtctctgac ctctgcccca     4620

gctccaaggt cagcaggcag ggagggctgt gtgtttgctg tttgctgctt gcaatgtttg     4680

cccattttag ggacatgagt aggctgaagt ttgttcagtg tggacttcag aggcagcaca     4740

caaacagcaa gcttgcgaat tccagtctac agagaggtct ctgacctctg ccccagctcc     4800

aaggtcagca ggcagggagg gctgtgtgtt tgctgtttgc tgcttgcaat gtttgcccat     4860

tttagggaca tgagtaggct gaagtttgtt cagtgtggac ttcagaggca gcacacaaac     4920

agcaagcttt gctctagact ggaattcgtc gacgagctcc ctatagtgag tcgtattaga     4980

ggccgactga cccggtaccc ggggatcttg ctaccagtgg aacagccact aaggattctg     5040

cagtgagagc agagggccag ctaagtggta ctctcccaga gactgtctga ctcacgccac     5100

cccctccacc ttggacacag gacgctgtgg tttctgagcc aggtacaatg actcctttcg     5160

gtaagtgcag tggaagctgt acactgccca ggcaaagcgt ccgggcagcg taggcgggcg     5220

actcagatcc cagccagtgg acttagcccc tgtttgctcc tccgataact ggggtgacct     5280

tggttaatat tcaccagcag cctcccccgt tgcccctctg gatccactgc ttaaatacgg     5340

acgaggacag ggccctgtct cctcagcttc aggcaccacc actgacctgg gacagtgaat     5400

gtccccctga tctgcggccg tgactctctt aaggtagcct tgcagaagtt ggtcgtgagg     5460

cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat agaaactggg     5520

cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc ttactgacat     5580

ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct cttaaggcta     5640

gagtacttaa tacgactcac tataggctag cctcgacctc gaggggccac catggccgat     5700

gaagtggctc tggcactgca ggccgctggc agtccttctg ccgcagctgc catggaagcc     5760

gcctctcagc ctgccgacga gcccctgaga aagaggccca gaagggacgg ccctggcctg     5820

ggaagaagcc ctggcgagcc ttcagctgcc gtggctcctg ccgctgccgg ctgtgaagcc     5880

gcttctgctg ctgcccctgc cgctctgtgg cgagaagctg ctggcgccgc tgcctctgcc     5940

gagagagagg ctcctgctac agccgtggcc ggagacggcg ataacggcag cggcctgaga     6000

agagagccta gagccgccga cgacttcgac gacgacgagg gcgaggaaga ggacgaggcc     6060

gctgctgccg cagcagctgc cgccatcggc tacagagaca acctgctgct gaccgacggc     6120

ctgctgacaa acggcttcca cagctgcgag agcgacgacg atgacagaac cagccacgcc     6180

tctagcagcg actggacccc cagacccaga atcggcccct acaccttcgt gcagcagcac     6240

ctgatgatcg gcaccgaccc cagaaccatc ctgaaggacc tgctgcccga gacaatcccc     6300

ccacccgagc tggacgacat gaccctgtgg cagatcgtga tcaacatcct gagcgagccc     6360

cccaagagaa agaagcgcaa ggacatcaac accatcgagg acgccgtgaa gctgctgcag     6420

gaatgcaaga aaatcatcgt gctgaccggc gctggcgtgt ccgtgtcttg cggcatcccc     6480

gacttcagaa gcagggacgg catctacgcc agactggccg tggacttccc cgacctgccc     6540

gatcctcagg ccatgttcga catcgagtac ttcagaaagg accccaggcc attcttcaag     6600

ttcgccaaag agatctaccc cggccagttc cagcccagcc tgtgccacaa gtttatcgcc     6660

ctgagcgaca aagagggcaa gctgctgaga aactacaccc agaacatcga caccctggaa     6720

caggtggccg gcatccagag aatcctgcag tgtcacggca gcttcgccac cgccagctgc     6780

ctgatctgca agtacaaggt ggactgcgag gccgtgcggg gcgacatctt caaccaggtg     6840

gtgcccagat gccccaggtg ccctgccgat gagcctctgg ccatcatgaa gcccgagatc     6900

gtgttcttcg gcgagaacct gcccgagcag ttccacagag ccatgaagta cgacaaggac     6960

gaggtggacc tgctgatcgt gatcggcagc agcctgaaag tgcggcccgt ggccctgatc     7020

cccagctcta tccctcacga ggtgccccag atcctgatca atagagagcc cctgccccat     7080

ctgcacttcg acgtcgagct gctgggcgac tgcgacgtga tcatcaacga gctgtgccac     7140

agactgggcg gcgagtacgc caagctgtgc tgcaaccctg tgaagctgag cgagatcacc     7200

gagaagcccc ccaggcccca gaaagaactg gtgcacctgt ccgagctgcc ccccacccct     7260

ctgcacatca gcgaggacag cagcagcccc gagagaaccg tgccccagga cagctccgtg     7320

atcgccacac tggtggacca ggccaccaac aacaacgtga acgacctgga agtgtccgag     7380

agcagctgcg tggaagagaa gccccaggaa gtgcagacca gcagaaacgt ggaaaacatc     7440

aacgtcgaga accctgactt caaggccgtg ggcagctcca ccgccgacaa gaacgagaga     7500

accagcgtgg ccgagacagt gcggaagtgc tggcccaaca gactggccaa agagcagatc     7560

agcaagagac tggaaggcaa ccagtacctg ttcgtgcccc ccaaccggta catcttccac     7620

ggcgccgagg tgtacagcga cagcgaggac gacgtgctga gcagctccag ctgcggcagc     7680

aactccgaca gcggcacctg tcagagcccc agcctggaag aacctctgga agatgagagc     7740

gagatcgagg aattctacaa cggcctcgag gacgacaccg agaggcctga gtgtgctggc     7800

ggctctggct ttggcgccga cggcggagat caggaagtgg tcaacgaggc tatcgccacc     7860

agacaggaac tgaccgacgt caactacccc agcgacaaga gctgaccc                  7908


<210> 2
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="36b4 Forward primer"
      /organism="Artificial Sequence"

<400> 2
tcccaccttg tctccagtct                                                20


<210> 3
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /mol_type="unassigned DNA"
      /note="36b4 Reverse Primer"
      /organism="Artificial Sequence"

<400> 3
actggtctag gacccgagaa g                                              21


<210> 4
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..19
<223> /mol_type="unassigned DNA"
      /note="Acadl Forward Primer"
      /organism="Artificial Sequence"

<400> 4
cccatggcat tagcctctt                                                 19


<210> 5
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..24
<223> /mol_type="unassigned DNA"
      /note="Acadl Reverse Primer"
      /organism="Artificial Sequence"

<400> 5
agaatagttc tgctgtgtcc tgag                                           24


<210> 6
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..22
<223> /mol_type="unassigned DNA"
      /note="Acadm Forward Primer"
      /organism="Artificial Sequence"

<400> 6
agtaccctgt ggagaagctg at                                             22


<210> 7
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /mol_type="unassigned DNA"
      /note="Acadm Reverse Primer"
      /organism="Artificial Sequence"

<400> 7
tcaatgtgct cacgagctat g                                              21


<210> 8
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..19
<223> /mol_type="unassigned DNA"
      /note="Acadvl Forward Primer"
      /organism="Artificial Sequence"

<400> 8
ggtggtttgg gcctctcta                                                 19


<210> 9
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="Acadvl Reverse Primer"
      /organism="Artificial Sequence"

<400> 9
gggtaacgct aacaccaagg                                                20


<210> 10
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /mol_type="unassigned DNA"
      /note="Adipoq Forward Primer"
      /organism="Artificial Sequence"

<400> 10
tgttcctctt aatcctgccc a                                              21


<210> 11
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /mol_type="unassigned DNA"
      /note="Adipoq Reverse Primer"
      /organism="Artificial Sequence"

<400> 11
ccaacctgca caagttccct t                                              21


<210> 12
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="Ccl2 Forward Primer"
      /organism="Artificial Sequence"

<400> 12
cccaatgagt aggctggaga                                                20


<210> 13
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="Ccl2 Reverse Primer"
      /organism="Artificial Sequence"

<400> 13
tctggaccca ttccttcttg                                                20


<210> 14
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..22
<223> /mol_type="unassigned DNA"
      /note="Ccl5 Forward Primer"
      /organism="Artificial Sequence"

<400> 14
cctcaccatc atcctcactg ca                                             22


<210> 15
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..22
<223> /mol_type="unassigned DNA"
      /note="Ccl5 Reverse Primer"
      /organism="Artificial Sequence"

<400> 15
tcttctctgg gttggcacac ac                                             22


<210> 16
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="Cd68 Forward Primer"
      /organism="Artificial Sequence"

<400> 16
ggggctcttg ggaactacac                                                20


<210> 17
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="Cd68 Reverse Primer"
      /organism="Artificial Sequence"

<400> 17
caagccctct ttaagcccca                                                20


<210> 18
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..18
<223> /mol_type="unassigned DNA"
      /note="Cpt2 Forward Primer"
      /organism="Artificial Sequence"

<400> 18
ccaaagaagc agcgatgg                                                  18


<210> 19
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..19
<223> /mol_type="unassigned DNA"
      /note="Cpt2 Reverse Primer"
      /organism="Artificial Sequence"

<400> 19
tagagctcag gcagggtga                                                 19


<210> 20
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..22
<223> /mol_type="unassigned DNA"
      /note="F4/80 Forward Primer"
      /organism="Artificial Sequence"

<400> 20
ctttggctat gggcttccag tc                                             22


<210> 21
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /mol_type="unassigned DNA"
      /note="F4/80 Reverse Primer"
      /organism="Artificial Sequence"

<400> 21
gcaaggagga cagagtttat c                                              21


<210> 22
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..24
<223> /mol_type="unassigned DNA"
      /note="Hif1a Forward Primer"
      /organism="Artificial Sequence"

<400> 22
gcactagaca aagttcacct gaga                                           24


<210> 23
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="Hif1a-Reverse Primer"
      /organism="Artificial Sequence"

<400> 23
cgctatccac atcaaagcaa                                                20


<210> 24
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /mol_type="unassigned DNA"
      /note="Il1b Forward Primer"
      /organism="Artificial Sequence"

<400> 24
tgtaatgaaa gacggcacac c                                              21


<210> 25
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /mol_type="unassigned DNA"
      /note="Il1b Reverse Primer"
      /organism="Artificial Sequence"

<400> 25
tcttctttgg gtattgcttg g                                              21


<210> 26
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /mol_type="unassigned DNA"
      /note="Il10 Forward Primer"
      /organism="Artificial Sequence"

<400> 26
ctatgctgcc tgctcttact g                                              21


<210> 27
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..22
<223> /mol_type="unassigned DNA"
      /note="Il10 Reverse Primer"
      /organism="Artificial Sequence"

<400> 27
aacccaagta acccttaaag tc                                             22


<210> 28
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..19
<223> /mol_type="unassigned DNA"
      /note="Lep Forward Primer"
      /organism="Artificial Sequence"

<400> 28
gagacccctg tgtcggttc                                                 19


<210> 29
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..22
<223> /mol_type="unassigned DNA"
      /note="Lep Reverse Primer"
      /organism="Artificial Sequence"

<400> 29
ctgcgtgtgt gaaatgtcat tg                                             22


<210> 30
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="mCpt1 Forward Primer"
      /organism="Artificial Sequence"

<400> 30
gcacaccagg cagtagcttt                                                20


<210> 31
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..23
<223> /mol_type="unassigned DNA"
      /note="mCpt1 Reverse Primer"
      /organism="Artificial Sequence"

<400> 31
caggagttga ttccagacag gta                                            23


<210> 32
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..18
<223> /mol_type="unassigned DNA"
      /note="Nos2 Forward Primer"
      /organism="Artificial Sequence"

<400> 32
ctttgccacg gacgagac                                                  18


<210> 33
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..22
<223> /mol_type="unassigned DNA"
      /note="Nos2 Reverse Primer"
      /organism="Artificial Sequence"

<400> 33
tcattgtact ctgagggctg ac                                             22


<210> 34
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="Nrf1 Forward Primer"
      /organism="Artificial Sequence"

<400> 34
ctgcaggtcc tgtgggaatg                                                20


<210> 35
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="Nrf1 Reverse Primer"
      /organism="Artificial Sequence"

<400> 35
actcgcgtcg tgtactcatc                                                20


<210> 36
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="Pdk4 Forward Primer"
      /organism="Artificial Sequence"

<400> 36
ccttcaccac atgctcttcg                                                20


<210> 37
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="Pdk4 Reverse Primer"
      /organism="Artificial Sequence"

<400> 37
cggttttctt gatgctcgac                                                20


<210> 38
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..22
<223> /mol_type="unassigned DNA"
      /note="Ppargc1a Forward Primer"
      /organism="Artificial Sequence"

<400> 38
ataccgcaaa gagcacgaga ag                                             22


<210> 39
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..25
<223> /mol_type="unassigned DNA"
      /note="Ppargc1a Reverse Primer"
      /organism="Artificial Sequence"

<400> 39
ctcaagagca gcgaaagcgt cacag                                          25


<210> 40
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..19
<223> /mol_type="unassigned DNA"
      /note="Ppara Forward Primer"
      /organism="Artificial Sequence"

<400> 40
tcggcgaact attcggctg                                                 19


<210> 41
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="Ppara Reverse Primer"
      /organism="Artificial Sequence"

<400> 41
gcacttgtga aaacggcagt                                                20


<210> 42
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /mol_type="unassigned DNA"
      /note="Ppard Forward Primer"
      /organism="Artificial Sequence"

<400> 42
tccatcgtca acaaagacgg g                                              21


<210> 43
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /mol_type="unassigned DNA"
      /note="Ppard Reverse Primer"
      /organism="Artificial Sequence"

<400> 43
acttgggctc aatgatgtca c                                              21


<210> 44
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..18
<223> /mol_type="unassigned DNA"
      /note="Sirt3 Forward Primer"
      /organism="Artificial Sequence"

<400> 44
tcctctgaaa ccggatgg                                                  18


<210> 45
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="Sirt3 Reverse Primer"
      /organism="Artificial Sequence"

<400> 45
tcccacacag agggatatgg                                                20


<210> 46
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..19
<223> /mol_type="unassigned DNA"
      /note="Sirt4 Forward Primer"
      /organism="Artificial Sequence"

<400> 46
tgatgtccaa aggctggaa                                                 19


<210> 47
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..18
<223> /mol_type="unassigned DNA"
      /note="Sirt4 Reverse Primer"
      /organism="Artificial Sequence"

<400> 47
agagttggag cggcattg                                                  18


<210> 48
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..19
<223> /mol_type="unassigned DNA"
      /note="Sirt6 Forward Primer"
      /organism="Artificial Sequence"

<400> 48
gacctgatgc tcgctgatg                                                 19


<210> 49
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="Sirt6 Reverse Primer"
      /organism="Artificial Sequence"

<400> 49
ggtacccagg gtgacagaca                                                20


<210> 50
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..25
<223> /mol_type="unassigned DNA"
      /note="Tnfa Forward Primer"
      /organism="Artificial Sequence"

<400> 50
catcttctca aaattcgagt gacaa                                          25


<210> 51
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..23
<223> /mol_type="unassigned DNA"
      /note="Tnfa Reverse Primer"
      /organism="Artificial Sequence"

<400> 51
tgggagtaga caaggtacaa ccc                                            23


<210> 52
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="Ucp2 Forward Primer"
      /organism="Artificial Sequence"

<400> 52
actttccctc tggataccgc                                                20


<210> 53
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="Ucp2 Reverse Primer"
      /organism="Artificial Sequence"

<400> 53
acggaggcaa agctcatctg                                                20


<210> 54
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="Ucp3 Forward Primer"
      /organism="Artificial Sequence"

<400> 54
ctgcaccgcc agatgagttt                                                20


<210> 55
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /mol_type="unassigned DNA"
      /note="Ucp3 Reverse Primer"
      /organism="Artificial Sequence"

<400> 55
atcatggctt gaaatcggac c                                              21


