                         SEQUENCE LISTING

<110>  Gotovax AB
 
<120>  CHOLERA TOXIN A-LIKE POLYPEPTIDE USEFUL AS ADJUVANT COMPONENT

<130>  NP0107WO

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  240
<212>  PRT
<213>  Vibrio cholerae

<400>  1

Asn Asp Asp Lys Leu Tyr Arg Ala Asp Ser Arg Pro Pro Asp Glu Ile 
1               5                   10                  15      


Lys Gln Ser Gly Gly Leu Met Pro Arg Gly Gln Ser Glu Tyr Phe Asp 
            20                  25                  30          


Arg Gly Thr Gln Met Asn Ile Asn Leu Tyr Asp His Ala Arg Gly Thr 
        35                  40                  45              


Gln Thr Gly Phe Val Arg His Asp Asp Gly Tyr Val Ser Thr Ser Ile 
    50                  55                  60                  


Ser Leu Arg Ser Ala His Leu Val Gly Gln Thr Ile Leu Ser Gly His 
65                  70                  75                  80  


Ser Thr Tyr Tyr Ile Tyr Val Ile Ala Thr Ala Pro Asn Met Phe Asn 
                85                  90                  95      


Val Asn Asp Val Leu Gly Ala Tyr Ser Pro His Pro Asp Glu Gln Glu 
            100                 105                 110         


Val Ser Ala Leu Gly Gly Ile Pro Tyr Ser Gln Ile Tyr Gly Trp Tyr 
        115                 120                 125             


Arg Val His Phe Gly Val Leu Asp Glu Gln Leu His Arg Asn Arg Gly 
    130                 135                 140                 


Tyr Arg Asp Arg Tyr Tyr Ser Asn Leu Asp Ile Ala Pro Ala Ala Asp 
145                 150                 155                 160 


Gly Tyr Gly Leu Ala Gly Phe Pro Pro Glu His Arg Ala Trp Arg Glu 
                165                 170                 175     


Glu Pro Trp Ile His His Ala Pro Pro Gly Cys Gly Asn Ala Pro Arg 
            180                 185                 190         


Ser Ser Met Ser Asn Thr Cys Asp Glu Lys Thr Gln Ser Leu Gly Val 
        195                 200                 205             


Lys Phe Leu Asp Glu Tyr Gln Ser Lys Val Lys Arg Gln Ile Phe Ser 
    210                 215                 220                 


Gly Tyr Gln Ser Asp Ile Asp Thr His Asn Arg Ile Lys Asp Glu Leu 
225                 230                 235                 240 


<210>  2
<211>  103
<212>  PRT
<213>  Vibrio cholerae

<400>  2

Thr Pro Gln Asn Ile Thr Asp Leu Cys Ala Glu Tyr His Asn Thr Gln 
1               5                   10                  15      


Ile His Thr Leu Asn Asp Lys Ile Phe Ser Tyr Thr Glu Ser Leu Arg 
            20                  25                  30          


Gly Lys Arg Glu Met Ala Ile Ile Thr Phe Lys Asn Gly Ala Thr Phe 
        35                  40                  45              


Gln Val Glu Val Pro Gly Ser Gln His Ile Asp Ser Gln Lys Lys Ala 
    50                  55                  60                  


Ile Glu Arg Met Lys Asp Thr Leu Arg Ile Ala Tyr Leu Thr Glu Ala 
65                  70                  75                  80  


Lys Val Glu Lys Leu Cys Val Trp Asn Asn Lys Thr Pro His Ala Ile 
                85                  90                  95      


Ala Ala Ile Ser Met Ala Asn 
            100             


<210>  3
<211>  240
<212>  PRT
<213>  Escherichia coli

<400>  3

Asn Gly Asp Lys Leu Tyr Arg Ala Asp Ser Arg Pro Pro Asp Glu Ile 
1               5                   10                  15      


Lys Arg Ser Gly Gly Leu Met Pro Arg Gly His Asn Glu Tyr Phe Asp 
            20                  25                  30          


Arg Gly Thr Gln Met Asn Ile Asn Leu Tyr Asp His Ala Arg Gly Thr 
        35                  40                  45              


Gln Thr Gly Phe Val Arg Tyr Asp Asp Gly Tyr Val Ser Thr Ser Leu 
    50                  55                  60                  


Ser Leu Arg Ser Ala His Leu Ala Gly Gln Ser Ile Leu Ser Gly Tyr 
65                  70                  75                  80  


Ser Thr Tyr Tyr Ile Tyr Val Ile Ala Thr Ala Pro Asn Met Phe Asn 
                85                  90                  95      


Val Asn Asp Val Leu Gly Val Tyr Ser Pro His Pro Tyr Glu Gln Glu 
            100                 105                 110         


Val Ser Ala Leu Gly Gly Ile Pro Tyr Ser Gln Ile Tyr Gly Trp Tyr 
        115                 120                 125             


Arg Val Asn Phe Gly Val Ile Asp Glu Arg Leu His Arg Asn Arg Glu 
    130                 135                 140                 


Tyr Arg Asp Arg Tyr Tyr Arg Asn Leu Asn Ile Ala Pro Ala Glu Asp 
145                 150                 155                 160 


Gly Tyr Arg Leu Ala Gly Phe Pro Pro Asp His Gln Ala Trp Arg Glu 
                165                 170                 175     


Glu Pro Trp Ile His His Ala Pro Gln Gly Cys Gly Asp Ser Ser Arg 
            180                 185                 190         


Thr Ile Thr Gly Asp Thr Cys Asn Glu Glu Thr Gln Asn Leu Ser Thr 
        195                 200                 205             


Ile Tyr Leu Arg Lys Tyr Gln Ser Lys Val Lys Arg Gln Ile Phe Ser 
    210                 215                 220                 


Asp Tyr Gln Ser Glu Val Asp Ile Tyr Asn Arg Ile Arg Asn Glu Leu 
225                 230                 235                 240 


<210>  4
<211>  103
<212>  PRT
<213>  Escherichia coli

<400>  4

Ala Pro Gln Ser Ile Thr Glu Leu Cys Ser Glu Tyr Arg Asn Thr Gln 
1               5                   10                  15      


Ile Tyr Thr Ile Asn Asp Lys Ile Leu Ser Tyr Thr Glu Ser Met Ala 
            20                  25                  30          


Gly Lys Arg Glu Met Val Ile Ile Thr Phe Lys Ser Gly Ala Thr Phe 
        35                  40                  45              


Gln Val Glu Val Pro Gly Ser Gln His Ile Asp Ser Gln Lys Lys Ala 
    50                  55                  60                  


Ile Glu Arg Met Lys Asp Thr Leu Arg Ile Thr Tyr Leu Thr Glu Thr 
65                  70                  75                  80  


Lys Ile Asp Lys Leu Cys Val Trp Asn Asn Lys Thr Pro Asn Ser Ile 
                85                  90                  95      


Ala Ala Ile Ser Met Glu Asn 
            100             


<210>  5
<211>  240
<212>  PRT
<213>  Escherichia coli

<400>  5

Asn Gly Asp Lys Leu Tyr Arg Ala Asp Ser Arg Pro Pro Asp Glu Ile 
1               5                   10                  15      


Lys Arg Ser Gly Gly Leu Met Pro Arg Gly His Asn Glu Tyr Phe Asp 
            20                  25                  30          


Arg Gly Thr Gln Met Asn Ile Asn Leu Tyr Asp His Ala Arg Gly Thr 
        35                  40                  45              


Gln Thr Gly Phe Val Arg Tyr Asp Asp Gly Tyr Val Ser Thr Ser Leu 
    50                  55                  60                  


Ser Leu Arg Ser Ala His Leu Ala Gly Gln Ser Ile Leu Ser Gly Tyr 
65                  70                  75                  80  


Ser Thr Tyr Tyr Ile Tyr Val Ile Ala Thr Ala Pro Asn Met Phe Asn 
                85                  90                  95      


Val Asn Asp Val Leu Gly Val Tyr Ser Pro His Pro Tyr Glu Gln Glu 
            100                 105                 110         


Val Ser Ala Leu Gly Gly Ile Pro Tyr Ser Gln Ile Tyr Gly Trp Tyr 
        115                 120                 125             


Arg Val Asn Phe Gly Val Ile Asp Glu Arg Leu His Arg Asn Arg Glu 
    130                 135                 140                 


Tyr Arg Asp Arg Tyr Tyr Arg Asn Leu Asn Ile Ala Pro Ala Glu Asp 
145                 150                 155                 160 


Gly Tyr Arg Leu Ala Gly Phe Pro Pro Asp His Gln Ala Trp Arg Glu 
                165                 170                 175     


Glu Pro Trp Ile His His Ala Pro Gln Gly Cys Gly Asp Ser Ser Gly 
            180                 185                 190         


Thr Ile Thr Gly Asp Thr Cys Asn Glu Glu Thr Gln Asn Leu Ser Thr 
        195                 200                 205             


Ile Tyr Ala Arg Lys Tyr Gln Ser Lys Val Lys Arg Gln Ile Phe Ser 
    210                 215                 220                 


Asp Tyr Gln Ser Glu Val Asp Ile Tyr Asn Arg Ile Arg Asn Glu Leu 
225                 230                 235                 240 


<210>  6
<211>  240
<212>  PRT
<213>  Vibrio cholerae

<400>  6

Asn Asp Asp Lys Leu Tyr Arg Ala Asp Ser Arg Pro Pro Asp Glu Ile 
1               5                   10                  15      


Lys Gln Ser Gly Gly Leu Met Pro Arg Gly Gln Ser Glu Tyr Phe Asp 
            20                  25                  30          


Arg Gly Thr Gln Met Asn Ile Asn Leu Tyr Asp His Ala Arg Gly Thr 
        35                  40                  45              


Gln Thr Gly Phe Val Arg His Asp Asp Gly Tyr Val Ser Thr Ser Ile 
    50                  55                  60                  


Ser Leu Arg Ser Ala His Leu Val Gly Gln Thr Ile Leu Ser Gly His 
65                  70                  75                  80  


Ser Thr Tyr Tyr Ile Tyr Val Ile Ala Thr Ala Pro Asn Met Phe Asn 
                85                  90                  95      


Val Asn Asp Val Leu Gly Ala Tyr Ser Pro His Pro Asp Glu Gln Glu 
            100                 105                 110         


Val Ser Ala Leu Gly Gly Ile Pro Tyr Ser Gln Ile Tyr Gly Trp Tyr 
        115                 120                 125             


Arg Val His Phe Gly Val Leu Asp Glu Gln Leu His Arg Asn Arg Gly 
    130                 135                 140                 


Tyr Arg Asp Arg Tyr Tyr Ser Asn Leu Asp Ile Ala Pro Ala Ala Asp 
145                 150                 155                 160 


Gly Tyr Gly Leu Ala Gly Phe Pro Pro Glu His Arg Ala Trp Arg Glu 
                165                 170                 175     


Glu Pro Trp Ile His His Ala Pro Pro Gly Cys Gly Asn Ala Pro Gly 
            180                 185                 190         


Ser Ser Met Ser Asn Thr Cys Asp Glu Lys Thr Gln Ser Leu Gly Val 
        195                 200                 205             


Lys Phe Ala Asp Glu Tyr Gln Ser Lys Val Lys Arg Gln Ile Phe Ser 
    210                 215                 220                 


Gly Tyr Gln Ser Asp Ile Asp Thr His Asn Arg Ile Lys Asp Glu Leu 
225                 230                 235                 240 


<210>  7
<211>  240
<212>  PRT
<213>  Vibrio cholerae

<400>  7

Asn Asp Asp Lys Leu Tyr Arg Ala Asp Ser Arg Pro Pro Asp Glu Ile 
1               5                   10                  15      


Lys Gln Ser Gly Gly Leu Met Pro Arg Gly Gln Ser Glu Tyr Phe Asp 
            20                  25                  30          


Arg Gly Thr Gln Met Asn Ile Asn Leu Tyr Asp His Ala Arg Gly Thr 
        35                  40                  45              


Gln Thr Gly Phe Val Arg His Asp Asp Gly Tyr Val Ser Thr Ser Ile 
    50                  55                  60                  


Ser Leu Arg Ser Ala His Leu Val Gly Gln Thr Ile Leu Ser Gly His 
65                  70                  75                  80  


Ser Thr Tyr Tyr Ile Tyr Val Ile Ala Thr Ala Pro Asn Met Phe Asn 
                85                  90                  95      


Val Asn Asp Val Leu Gly Ala Tyr Ser Pro His Pro Asp Glu Gln Glu 
            100                 105                 110         


Val Ser Ala Leu Gly Gly Ile Pro Tyr Ser Gln Ile Tyr Gly Trp Tyr 
        115                 120                 125             


Arg Val His Phe Gly Val Leu Asp Glu Gln Leu His Arg Asn Arg Gly 
    130                 135                 140                 


Tyr Arg Asp Arg Tyr Tyr Ser Asn Leu Asp Ile Ala Pro Ala Ala Asp 
145                 150                 155                 160 


Gly Tyr Gly Leu Ala Gly Phe Pro Pro Glu His Arg Ala Trp Arg Glu 
                165                 170                 175     


Glu Pro Trp Ile His His Ala Pro Pro Gly Cys Gly Asp Ser Ser Gly 
            180                 185                 190         


Thr Ile Thr Gly Asp Thr Cys Asp Glu Lys Thr Gln Ser Leu Gly Val 
        195                 200                 205             


Lys Phe Ala Asp Glu Tyr Gln Ser Lys Val Lys Arg Gln Ile Phe Ser 
    210                 215                 220                 


Gly Tyr Gln Ser Asp Ile Asp Thr His Asn Arg Ile Lys Asp Glu Leu 
225                 230                 235                 240 


<210>  8
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  dm CTA replaced portion

<400>  8

Asn Ala Pro Gly Ser Ser Met Ser Asn 
1               5                   


<210>  9
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  Mutated section

<400>  9

Asp Ser Ser Gly Thr Ile Thr Gly Asp 
1               5                   


<210>  10
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  10
gggggtctct ctagaatgct gcgggagcaa ggcggctg                               38


<210>  11
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  11
ggggggaagc ttataattca tccttaattc tattatgtgt atcaatatca gattg            55


<210>  12
<211>  609
<212>  PRT
<213>  Vibrio cholerae

<400>  12

Met Lys Met Ile Gln Arg Pro Leu Asn Trp Leu Val Leu Ala Gly Ala 
1               5                   10                  15      


Ala Thr Gly Phe Pro Leu Tyr Ala Ala Gln Met Val Met Ile Asp Asp 
            20                  25                  30          


Ala Ser Met Val Glu Gln Ala Leu Ala Gln Gln Gln Tyr Ser Met Met 
        35                  40                  45              


Pro Ala Ala Ser Gly Phe Lys Ala Val Asn Thr Val Gln Leu Pro Asn 
    50                  55                  60                  


Gly Lys Val Lys Val Arg Tyr Gln Gln Met Tyr Asn Gly Val Pro Val 
65                  70                  75                  80  


Tyr Gly Thr Ala Val Val Ala Thr Glu Ser Ser Lys Gly Ile Ser Gln 
                85                  90                  95      


Val Tyr Gly Gln Met Ala Gln Gln Leu Glu Ala Asp Leu Ser Thr Val 
            100                 105                 110         


Thr Pro Asp Ile Glu Ser Gln Gln Ala Ile Ala Leu Ala Val Ser His 
        115                 120                 125             


Phe Gly Glu Gln His Ala Gly Glu Ser Leu Pro Val Glu Asn Glu Ser 
    130                 135                 140                 


Val Gln Leu Met Val Arg Leu Asp Asp Asn Gln Gln Ala Gln Leu Val 
145                 150                 155                 160 


Tyr Leu Val Asp Phe Phe Val Ala Ser Glu Thr Pro Ser Arg Pro Phe 
                165                 170                 175     


Tyr Phe Ile Ser Ala Ala Thr Gly Glu Val Leu Asp Gln Trp Asp Gly 
            180                 185                 190         


Ile Asn His Ala Gln Ala Thr Gly Thr Gly Pro Gly Gly Asn Gln Lys 
        195                 200                 205             


Thr Gly Arg Tyr Glu Tyr Gly Ser Asn Gly Leu Pro Gly Phe Thr Ile 
    210                 215                 220                 


Asp Lys Thr Gly Thr Thr Cys Thr Met Asn Asn Ser Ala Val Lys Thr 
225                 230                 235                 240 


Val Asn Leu Asn Gly Gly Thr Ser Gly Ser Thr Ala Phe Ser Tyr Ala 
                245                 250                 255     


Cys Asn Asn Ser Thr Asn Tyr Asn Ser Val Lys Thr Val Asn Gly Ala 
            260                 265                 270         


Tyr Ser Pro Leu Asn Asp Ala His Phe Phe Gly Lys Val Val Phe Asp 
        275                 280                 285             


Met Tyr Gln Gln Trp Leu Asn Thr Ser Pro Leu Thr Phe Gln Leu Thr 
    290                 295                 300                 


Met Arg Val His Tyr Gly Asn Asn Tyr Glu Asn Ala Phe Trp Asp Gly 
305                 310                 315                 320 


Arg Ala Met Thr Phe Gly Asp Gly Tyr Thr Arg Phe Tyr Pro Leu Val 
                325                 330                 335     


Asp Ile Asn Val Ser Ala His Glu Val Ser His Gly Phe Thr Glu Gln 
            340                 345                 350         


Asn Ser Gly Leu Val Tyr Arg Asp Met Ser Gly Gly Ile Asn Glu Ala 
        355                 360                 365             


Phe Ser Asp Ile Ala Gly Glu Ala Ala Glu Tyr Phe Met Arg Gly Asn 
    370                 375                 380                 


Val Asp Trp Ile Val Gly Ala Asp Ile Phe Lys Ser Ser Gly Gly Leu 
385                 390                 395                 400 


Arg Tyr Phe Asp Gln Pro Ser Arg Asp Gly Arg Ser Ile Asp His Ala 
                405                 410                 415     


Ser Gln Tyr Tyr Ser Gly Ile Asp Val His His Ser Ser Gly Val Phe 
            420                 425                 430         


Asn Arg Ala Phe Tyr Leu Leu Ala Asn Lys Ser Gly Trp Asn Val Arg 
        435                 440                 445             


Lys Gly Phe Glu Val Phe Ala Val Ala Asn Gln Leu Tyr Trp Thr Pro 
    450                 455                 460                 


Asn Ser Thr Phe Asp Gln Gly Gly Cys Gly Val Val Lys Ala Ala Gln 
465                 470                 475                 480 


Asp Leu Asn Tyr Asn Thr Ala Asp Val Val Ala Ala Phe Asn Thr Val 
                485                 490                 495     


Gly Val Asn Ala Ser Cys Gly Thr Thr Pro Pro Pro Val Gly Lys Val 
            500                 505                 510         


Leu Glu Lys Gly Lys Pro Phe Thr Gly Leu Ser Gly Ser Arg Gly Gly 
        515                 520                 525             


Glu Asp Phe Tyr Thr Phe Thr Val Thr Asn Ser Gly Ser Val Val Val 
    530                 535                 540                 


Ser Ile Ser Gly Gly Thr Gly Asp Ala Asp Leu Tyr Val Lys Ala Gly 
545                 550                 555                 560 


Ser Lys Pro Thr Thr Ser Ser Trp Asp Cys Arg Pro Tyr Arg Ser Gly 
                565                 570                 575     


Asn Ala Glu Gln Cys Ser Ile Ser Ala Val Val Gly Thr Thr Tyr His 
            580                 585                 590         


Val Met Leu Arg Gly Tyr Ser Asn Tyr Ser Gly Val Thr Leu Arg Leu 
        595                 600                 605             


Asp 
    


<210>  13
<211>  62
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  13
gcaagtatca cctgtaattg ttcctgatga atccccacaa cccggcggtg catgatgaat       60

cc                                                                      62


<210>  14
<211>  70
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  14
gattcatcag gaacaattac aggtgatact tgcgatgaaa aaacccaaag tctaggtgta       60

aaattcgctg                                                              70


<210>  15
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic

<400>  15
ggcgcccatg gtgaaaacgg gggcgaag                                          28


<210>  16
<211>  34
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  16
ggcgcccatg ggcaaatatt atacgcaagg cgac                                   34


