                         SEQUENCE LISTING

<110>  ISIS INNOVATION LIMITED
 
<120>  METHOD

<130>  N400889WO

<150>  UK 1316849.7
<151>  2013-09-23

<150>  US 61/896,933
<151>  2013-10-29

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  882
<212>  DNA
<213>  Staphylococcus aureus

<400>  1
atggcagatt ctgatattaa tattaaaacc ggtactacag atattggaag caatactaca       60

gtaaaaacag gtgatttagt cacttatgat aaagaaaatg gcatgcacaa aaaagtattt      120

tatagtttta tcgatgataa aaatcacaat aaaaaactgc tagttattag aacaaaaggt      180

accattgctg gtcaatatag agtttatagc gaagaaggtg ctaacaaaag tggtttagcc      240

tggccttcag cctttaaggt acagttgcaa ctacctgata atgaagtagc tcaaatatct      300

gattactatc caagaaattc gattgataca aaagagtata tgagtacttt aacttatgga      360

ttcaacggta atgttactgg tgatgataca ggaaaaattg gcggccttat tggtgcaaat      420

gtttcgattg gtcatacact gaaatatgtt caacctgatt tcaaaacaat tttagagagc      480

ccaactgata aaaaagtagg ctggaaagtg atatttaaca atatggtgaa tcaaaattgg      540

ggaccatacg atcgagattc ttggaacccg gtatatggca atcaactttt catgaaaact      600

agaaatggtt ctatgaaagc agcagataac ttccttgatc ctaacaaagc aagttctcta      660

ttatcttcag ggttttcacc agacttcgct acagttatta ctatggatag aaaagcatcc      720

aaacaacaaa caaatataga tgtaatatac gaacgagttc gtgatgatta ccaattgcat      780

tggacttcaa caaattggaa aggtaccaat actaaagata aatggacaga tcgttcttca      840

gaaagatata aaatcgattg ggaaaaagaa gaaatgacaa at                         882


<210>  2
<211>  293
<212>  PRT
<213>  Staphylococcus aureus

<400>  2

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Thr Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn 
    290             


<210>  3
<211>  903
<212>  DNA
<213>  Staphylococcus aureus

<400>  3
atggcggaag gcaaaattac cccggttagc gtgaaaaaag ttgatgacaa agtgaccctg       60

tataaaacga cggcgacggc ggatagcgat aaatttaaaa ttagccagat cctgaccttc      120

aacttcatca aagacaaatc ttatgataaa gacaccctgg ttctgaaagc gacgggcaac      180

atcaatagcg gttttgtcaa accgaacccg aatgattacg acttctcaaa actgtattgg      240

ggcgccaaat acaatgtctc gattagctct cagagtaacg attccgtgaa tgcggttgac      300

tatgccccga aaaaccaaaa cgaagaattc caggttcaaa acaccctggg ttacacgttc      360

ggcggtgata tttcaatctc gaatggcctg agtggcggtc tgaacggtaa taccgcattt      420

tccgaaacga ttaactataa acaggaaagc taccgtaccc tgtctcgcaa cacgaattat      480

aaaaacgtcg gctggggtgt ggaagcgcat aaaatcatga atggctgggg tccgtatggc      540

cgtgattcct ttcacccgac ctacggcaac gaactgttcc tggcaggtcg ccagagttcc      600

gcgtatgccg gtcaaaattt tattgctcag catcaaatgc cgctgctgag ccgttctaac      660

tttaatccgg aattcctgtc agtgctgtcg caccgtcagg atcgcgcgaa aaaatctaaa      720

atcaccgtta cgtaccagcg tgaaatggac ctgtaccaaa tccgctggaa tggcttctat      780

tgggcaggtg ctaactacaa aaattttaaa acccgcacgt tcaaatctac ctatgaaatc      840

gattgggaaa atcacaaagt caaactgctg gacaccaaag aaaccgaaaa caacaaataa      900

taa                                                                    903


<210>  4
<211>  298
<212>  PRT
<213>  Staphylococcus aureus

<400>  4

Ala Glu Gly Lys Ile Thr Pro Val Ser Val Lys Lys Val Asp Asp Lys 
1               5                   10                  15      


Val Thr Leu Tyr Lys Thr Thr Ala Thr Ala Asp Ser Asp Lys Phe Lys 
            20                  25                  30          


Ile Ser Gln Ile Leu Thr Phe Asn Phe Ile Lys Asp Lys Ser Tyr Asp 
        35                  40                  45              


Lys Asp Thr Leu Val Leu Lys Ala Thr Gly Asn Ile Asn Ser Gly Phe 
    50                  55                  60                  


Val Lys Pro Asn Pro Asn Asp Tyr Asp Phe Ser Lys Leu Tyr Trp Gly 
65                  70                  75                  80  


Ala Lys Tyr Asn Val Ser Ile Ser Ser Gln Ser Asn Asp Ser Val Asn 
                85                  90                  95      


Ala Val Asp Tyr Ala Pro Lys Asn Gln Asn Glu Glu Phe Gln Val Gln 
            100                 105                 110         


Asn Thr Leu Gly Tyr Thr Phe Gly Gly Asp Ile Ser Ile Ser Asn Gly 
        115                 120                 125             


Leu Ser Gly Gly Leu Asn Gly Asn Thr Ala Phe Ser Glu Thr Ile Asn 
    130                 135                 140                 


Tyr Lys Gln Glu Ser Tyr Arg Thr Leu Ser Arg Asn Thr Asn Tyr Lys 
145                 150                 155                 160 


Asn Val Gly Trp Gly Val Glu Ala His Lys Ile Met Asn Gly Trp Gly 
                165                 170                 175     


Pro Tyr Gly Arg Asp Ser Phe His Pro Thr Tyr Gly Asn Glu Leu Phe 
            180                 185                 190         


Leu Ala Gly Arg Gln Ser Ser Ala Tyr Ala Gly Gln Asn Phe Ile Ala 
        195                 200                 205             


Gln His Gln Met Pro Leu Leu Ser Arg Ser Asn Phe Asn Pro Glu Phe 
    210                 215                 220                 


Leu Ser Val Leu Ser His Arg Gln Asp Arg Ala Lys Lys Ser Lys Ile 
225                 230                 235                 240 


Thr Val Thr Tyr Gln Arg Glu Met Asp Leu Tyr Gln Ile Arg Trp Asn 
                245                 250                 255     


Gly Phe Tyr Trp Ala Gly Ala Asn Tyr Lys Asn Phe Lys Thr Arg Thr 
            260                 265                 270         


Phe Lys Ser Thr Tyr Glu Ile Asp Trp Glu Asn His Lys Val Lys Leu 
        275                 280                 285             


Leu Asp Thr Lys Glu Thr Glu Asn Asn Lys 
    290                 295             


<210>  5
<211>  849
<212>  DNA
<213>  Staphylococcus aureus

<400>  5
atggaaaaca aaatcgaaga catcggtcaa ggcgctgaaa tcatcaaacg cacgcaggac       60

attacctcta aacgtctggc tattacccag aatattcaat tcgatttcgt gaaagacaaa      120

aaatacaaca aagatgcact ggtggttaaa atgcagggct ttatcagctc tcgtaccacg      180

tacagcgatc tgaaaaaata tccgtacatt aaacgcatga tctggccgtt ccagtacaac      240

attagtctga aaaccaaaga ttccaacgtg gacctgatta attacctgcc gaaaaacaaa      300

atcgatagtg cggacgtttc ccagaaactg ggctataaca ttggcggtaa ttttcaatca      360

gccccgtcga tcggcggtag tggttccttc aattactcaa aaaccatctc gtacaaccag      420

aaaaattacg ttacggaagt cgaaagccaa aactctaaag gcgtgaaatg gggtgttaaa      480

gcgaattcat ttgtcacccc gaacggccag gtgtcggcgt atgatcagta cctgtttgca      540

caagacccga cgggtccggc agcacgtgat tatttcgttc cggacaatca gctgccgccg      600

ctgattcaaa gcggctttaa cccgtctttc atcaccacgc tgtcccatga acgtggcaaa      660

ggtgataaaa gcgaatttga aattacctat ggtcgcaaca tggatgcaac ctatgcttac      720

gttacgcgtc atcgcctggc agtcgatcgt aaacacgacg ctttcaaaaa ccgcaatgtc      780

accgtgaaat acgaagtcaa ctggaaaacg cacgaagtca aaatcaaaag tatcacgccg      840

aaataataa                                                              849


<210>  6
<211>  280
<212>  PRT
<213>  Staphylococcus aureus

<400>  6

Glu Asn Lys Ile Glu Asp Ile Gly Gln Gly Ala Glu Ile Ile Lys Arg 
1               5                   10                  15      


Thr Gln Asp Ile Thr Ser Lys Arg Leu Ala Ile Thr Gln Asn Ile Gln 
            20                  25                  30          


Phe Asp Phe Val Lys Asp Lys Lys Tyr Asn Lys Asp Ala Leu Val Val 
        35                  40                  45              


Lys Met Gln Gly Phe Ile Ser Ser Arg Thr Thr Tyr Ser Asp Leu Lys 
    50                  55                  60                  


Lys Tyr Pro Tyr Ile Lys Arg Met Ile Trp Pro Phe Gln Tyr Asn Ile 
65                  70                  75                  80  


Ser Leu Lys Thr Lys Asp Ser Asn Val Asp Leu Ile Asn Tyr Leu Pro 
                85                  90                  95      


Lys Asn Lys Ile Asp Ser Ala Asp Val Ser Gln Lys Leu Gly Tyr Asn 
            100                 105                 110         


Ile Gly Gly Asn Phe Gln Ser Ala Pro Ser Ile Gly Gly Ser Gly Ser 
        115                 120                 125             


Phe Asn Tyr Ser Lys Thr Ile Ser Tyr Asn Gln Lys Asn Tyr Val Thr 
    130                 135                 140                 


Glu Val Glu Ser Gln Asn Ser Lys Gly Val Lys Trp Gly Val Lys Ala 
145                 150                 155                 160 


Asn Ser Phe Val Thr Pro Asn Gly Gln Val Ser Ala Tyr Asp Gln Tyr 
                165                 170                 175     


Leu Phe Ala Gln Asp Pro Thr Gly Pro Ala Ala Arg Asp Tyr Phe Val 
            180                 185                 190         


Pro Asp Asn Gln Leu Pro Pro Leu Ile Gln Ser Gly Phe Asn Pro Ser 
        195                 200                 205             


Phe Ile Thr Thr Leu Ser His Glu Arg Gly Lys Gly Asp Lys Ser Glu 
    210                 215                 220                 


Phe Glu Ile Thr Tyr Gly Arg Asn Met Asp Ala Thr Tyr Ala Tyr Val 
225                 230                 235                 240 


Thr Arg His Arg Leu Ala Val Asp Arg Lys His Asp Ala Phe Lys Asn 
                245                 250                 255     


Arg Asn Val Thr Val Lys Tyr Glu Val Asn Trp Lys Thr His Glu Val 
            260                 265                 270         


Lys Ile Lys Ser Ile Thr Pro Lys 
        275                 280 


<210>  7
<211>  558
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MS-B1 mutant MspA monomer

<400>  7
atgggtctgg ataatgaact gagcctggtg gacggtcaag atcgtaccct gacggtgcaa       60

caatgggata cctttctgaa tggcgttttt ccgctggatc gtaatcgcct gacccgtgaa      120

tggtttcatt ccggtcgcgc aaaatatatc gtcgcaggcc cgggtgctga cgaattcgaa      180

ggcacgctgg aactgggtta tcagattggc tttccgtggt cactgggcgt tggtatcaac      240

ttctcgtaca ccacgccgaa tattctgatc aacaatggta acattaccgc accgccgttt      300

ggcctgaaca gcgtgattac gccgaacctg tttccgggtg ttagcatctc tgcccgtctg      360

ggcaatggtc cgggcattca agaagtggca acctttagtg tgcgcgtttc cggcgctaaa      420

ggcggtgtcg cggtgtctaa cgcccacggt accgttacgg gcgcggccgg cggtgtcctg      480

ctgcgtccgt tcgcgcgcct gattgcctct accggcgaca gcgttacgac ctatggcgaa      540

ccgtggaata tgaactaa                                                    558


<210>  8
<211>  184
<212>  PRT
<213>  Artificial sequence

<220>
<223>  MS-B1 mutant MspA monomer

<400>  8

Gly Leu Asp Asn Glu Leu Ser Leu Val Asp Gly Gln Asp Arg Thr Leu 
1               5                   10                  15      


Thr Val Gln Gln Trp Asp Thr Phe Leu Asn Gly Val Phe Pro Leu Asp 
            20                  25                  30          


Arg Asn Arg Leu Thr Arg Glu Trp Phe His Ser Gly Arg Ala Lys Tyr 
        35                  40                  45              


Ile Val Ala Gly Pro Gly Ala Asp Glu Phe Glu Gly Thr Leu Glu Leu 
    50                  55                  60                  


Gly Tyr Gln Ile Gly Phe Pro Trp Ser Leu Gly Val Gly Ile Asn Phe 
65                  70                  75                  80  


Ser Tyr Thr Thr Pro Asn Ile Leu Ile Asn Asn Gly Asn Ile Thr Ala 
                85                  90                  95      


Pro Pro Phe Gly Leu Asn Ser Val Ile Thr Pro Asn Leu Phe Pro Gly 
            100                 105                 110         


Val Ser Ile Ser Ala Arg Leu Gly Asn Gly Pro Gly Ile Gln Glu Val 
        115                 120                 125             


Ala Thr Phe Ser Val Arg Val Ser Gly Ala Lys Gly Gly Val Ala Val 
    130                 135                 140                 


Ser Asn Ala His Gly Thr Val Thr Gly Ala Ala Gly Gly Val Leu Leu 
145                 150                 155                 160 


Arg Pro Phe Ala Arg Leu Ile Ala Ser Thr Gly Asp Ser Val Thr Thr 
                165                 170                 175     


Tyr Gly Glu Pro Trp Asn Met Asn 
            180                 


<210>  9
<211>  184
<212>  PRT
<213>  Mycobacterium smegmatis

<400>  9

Gly Leu Asp Asn Glu Leu Ser Leu Val Asp Gly Gln Asp Arg Thr Leu 
1               5                   10                  15      


Thr Val Gln Gln Trp Asp Thr Phe Leu Asn Gly Val Phe Pro Leu Asp 
            20                  25                  30          


Arg Asn Arg Leu Thr Arg Glu Trp Phe His Ser Gly Arg Ala Lys Tyr 
        35                  40                  45              


Ile Val Ala Gly Pro Gly Ala Asp Glu Phe Glu Gly Thr Leu Glu Leu 
    50                  55                  60                  


Gly Tyr Gln Ile Gly Phe Pro Trp Ser Leu Gly Val Gly Ile Asn Phe 
65                  70                  75                  80  


Ser Tyr Thr Thr Pro Asn Ile Leu Ile Asp Asp Gly Asp Ile Thr Ala 
                85                  90                  95      


Pro Pro Phe Gly Leu Asn Ser Val Ile Thr Pro Asn Leu Phe Pro Gly 
            100                 105                 110         


Val Ser Ile Ser Ala Asp Leu Gly Asn Gly Pro Gly Ile Gln Glu Val 
        115                 120                 125             


Ala Thr Phe Ser Val Asp Val Ser Gly Pro Ala Gly Gly Val Ala Val 
    130                 135                 140                 


Ser Asn Ala His Gly Thr Val Thr Gly Ala Ala Gly Gly Val Leu Leu 
145                 150                 155                 160 


Arg Pro Phe Ala Arg Leu Ile Ala Ser Thr Gly Asp Ser Val Thr Thr 
                165                 170                 175     


Tyr Gly Glu Pro Trp Asn Met Asn 
            180                 


<210>  10
<211>  184
<212>  PRT
<213>  Mycobacterium smegmatis

<400>  10

Gly Leu Asp Asn Glu Leu Ser Leu Val Asp Gly Gln Asp Arg Thr Leu 
1               5                   10                  15      


Thr Val Gln Gln Trp Asp Thr Phe Leu Asn Gly Val Phe Pro Leu Asp 
            20                  25                  30          


Arg Asn Arg Leu Thr Arg Glu Trp Phe His Ser Gly Arg Ala Lys Tyr 
        35                  40                  45              


Ile Val Ala Gly Pro Gly Ala Asp Glu Phe Glu Gly Thr Leu Glu Leu 
    50                  55                  60                  


Gly Tyr Gln Ile Gly Phe Pro Trp Ser Leu Gly Val Gly Ile Asn Phe 
65                  70                  75                  80  


Ser Tyr Thr Thr Pro Asn Ile Leu Ile Asp Asp Gly Asp Ile Thr Gly 
                85                  90                  95      


Pro Pro Phe Gly Leu Glu Ser Val Ile Thr Pro Asn Leu Phe Pro Gly 
            100                 105                 110         


Val Ser Ile Ser Ala Asp Leu Gly Asn Gly Pro Gly Ile Gln Glu Val 
        115                 120                 125             


Ala Thr Phe Ser Val Asp Val Ser Gly Pro Ala Gly Gly Val Ala Val 
    130                 135                 140                 


Ser Asn Ala His Gly Thr Val Thr Gly Ala Ala Gly Gly Val Leu Leu 
145                 150                 155                 160 


Arg Pro Phe Ala Arg Leu Ile Ala Ser Thr Gly Asp Ser Val Thr Thr 
                165                 170                 175     


Tyr Gly Glu Pro Trp Asn Met Asn 
            180                 


<210>  11
<211>  183
<212>  PRT
<213>  Mycobacterium smegmatis

<400>  11

Val Asp Asn Gln Leu Ser Val Val Asp Gly Gln Gly Arg Thr Leu Thr 
1               5                   10                  15      


Val Gln Gln Ala Glu Thr Phe Leu Asn Gly Val Phe Pro Leu Asp Arg 
            20                  25                  30          


Asn Arg Leu Thr Arg Glu Trp Phe His Ser Gly Arg Ala Thr Tyr His 
        35                  40                  45              


Val Ala Gly Pro Gly Ala Asp Glu Phe Glu Gly Thr Leu Glu Leu Gly 
    50                  55                  60                  


Tyr Gln Val Gly Phe Pro Trp Ser Leu Gly Val Gly Ile Asn Phe Ser 
65                  70                  75                  80  


Tyr Thr Thr Pro Asn Ile Leu Ile Asp Gly Gly Asp Ile Thr Gln Pro 
                85                  90                  95      


Pro Phe Gly Leu Asp Thr Ile Ile Thr Pro Asn Leu Phe Pro Gly Val 
            100                 105                 110         


Ser Ile Ser Ala Asp Leu Gly Asn Gly Pro Gly Ile Gln Glu Val Ala 
        115                 120                 125             


Thr Phe Ser Val Asp Val Lys Gly Ala Lys Gly Ala Val Ala Val Ser 
    130                 135                 140                 


Asn Ala His Gly Thr Val Thr Gly Ala Ala Gly Gly Val Leu Leu Arg 
145                 150                 155                 160 


Pro Phe Ala Arg Leu Ile Ala Ser Thr Gly Asp Ser Val Thr Thr Tyr 
                165                 170                 175     


Gly Glu Pro Trp Asn Met Asn 
            180             


<210>  12
<211>  23
<212>  PRT
<213>  Artificial sequence

<220>
<223>  C terminus Trx mutant

<400>  12

Val Gly Ala Leu Ser Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn 
1               5                   10                  15      


Leu Ala Arg Arg Ala Ser Cys 
            20              


<210>  13
<211>  19
<212>  PRT
<213>  Artificial sequence

<220>
<223>  C terminus Trx mutant

<400>  13

Val Gly Ala Leu Ser Lys Gly Gln Leu Lys Glu Phe Leu Arg Arg Asn 
1               5                   10                  15      


Ser Ala Cys 
            


<210>  14
<211>  19
<212>  PRT
<213>  Artificial sequence

<220>
<223>  C terminus Trx mutant

<400>  14

Val Arg Arg Leu Ser Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn 
1               5                   10                  15      


Leu Ala Cys 
            


<210>  15
<211>  23
<212>  PRT
<213>  Artificial sequence

<220>
<223>  C terminus Trx mutant

<400>  15

Val Gly Ala Leu Ser Lys Gly Gln Leu Lys Glu Phe Leu Arg Arg Asn 
1               5                   10                  15      


Ser Ala Arg Arg Ala Ser Cys 
            20              


<210>  16
<211>  23
<212>  PRT
<213>  Artificial sequence

<220>
<223>  C terminus Trx mutant

<400>  16

Val Gly Ala Leu Ser Lys Gly Gln Leu Lys Glu Phe Leu Arg Arg Asn 
1               5                   10                  15      


Ala Ala Arg Arg Ala Ser Cys 
            20              


<210>  17
<211>  23
<212>  PRT
<213>  Artificial sequence

<220>
<223>  C terminus Trx mutant

<400>  17

Val Gly Ala Leu Ser Lys Gly Gln Leu Lys Glu Phe Leu Arg Arg Asn 
1               5                   10                  15      


Ser Ala Arg Arg Ala Ala Cys 
            20              


