                         LISTADO DE SECUENCIAS

<110>  Universidad de Castilla la Mancha
 
<120>  Biosensor para la deteccin de cido fosfatdico en membranas 
       celulares

<130>  PCT1874.26

<150>  ES P201331377

<151>  2013-09-23

<160>  41    

<170>  PatentIn version 3.5

<210>  1
<211>  684
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  ECFP

<400>  1
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtaa acggccacag gttcagcgtg tccggcgagg gcgagggcga tgccacctac      120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      180

ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc gctaccccga ccacatgaag      240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg taccatcttc      300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaactacat cagccacaac gtctatatca ccgccgacaa gcagaagaac      480

ggcatcaagg cccacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tcgtgaccgc cgcc                                             684


<210>  2
<211>  228
<212>  PRT
<213>  Secuencia artificial

<220>
<223>  ECFP

<400>  2

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Arg Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Trp Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Ile Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Ala His Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala 
225             


<210>  3
<211>  123
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  SPo20 fragmento 51-91

<400>  3
atggacaatt gttcaggaag cagaagacgt gataggctac atgtgaagct taaatccttg       60

aggaataaaa tccacaaaca acttcaccca aactgtcggt tcgatgacgc cactaagact      120

agt                                                                    123


<210>  4
<211>  41
<212>  PRT
<213>  Secuencia artificial

<220>
<223>  SPo20 fragmento 51-91

<400>  4

Met Asp Asn Cys Ser Gly Ser Arg Arg Arg Asp Arg Leu His Val Lys 
1               5                   10                  15      


Leu Lys Ser Leu Arg Asn Lys Ile His Lys Gln Leu His Pro Asn Cys 
            20                  25                  30          


Arg Phe Asp Asp Ala Thr Lys Thr Ser 
        35                  40      


<210>  5
<211>  717
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  Venus

<400>  5
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac      120

ggcaagctga ccctgaagct gatctgcacc accggcaagc tgcccgtgcc ctggcccacc      180

ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag      240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc      300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa gcagaagaac      480

ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaag         717


<210>  6
<211>  239
<212>  PRT
<213>  Secuencia artificial

<220>
<223>  Venus

<400>  6

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Leu Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Gly 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 


<210>  7
<211>  735
<212>  DNA
<213>  Secuencia artificial
<220>
<223>  Venus cp49

<400>  7
atgaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcgtg       60

atgtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg      120

cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc      180

cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc      240

gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac      300

aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc      360

cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc      420

ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc      480

aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg      540

atcactctcg gcatggacga gctgtacaag ggtggcagcg gtggcatggt gagcaagggc      600

gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc      660

cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg      720

aagctgatct gcacc                                                       735


<210>  8
<211>  245
<212>  PRT
<213>  Secuencia artificial

<220>
<223>  Venus cp49

<400>  8

Met Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 
1               5                   10                  15      


Gly Tyr Gly Val Met Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 
            20                  25                  30          


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
        35                  40                  45              


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
    50                  55                  60                  


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
65                  70                  75                  80  


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
                85                  90                  95      


Tyr Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly 
            100                 105                 110         


Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Gly Val 
        115                 120                 125             


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
    130                 135                 140                 


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Ala Leu Ser 
145                 150                 155                 160 


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
                165                 170                 175     


Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Gly Gly 
            180                 185                 190         


Ser Gly Gly Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val 
        195                 200                 205             


Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser 
    210                 215                 220                 


Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu 
225                 230                 235                 240 


Lys Leu Ile Cys Thr 
                245 


<210>  9
<211>  735
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  Venus cp157

<400>  9
atgcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc       60

gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg      120

cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc      180

gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag      240

ctgtacaagg gtggcagcgg tggcatggtg agcaagggcg aggagctgtt caccggggtg      300

gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc      360

gagggcgagg gcgatgccac ctacggcaag ctgaccctga agctgatctg caccaccggc      420

aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcct gatgtgcttc      480

gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc      540

tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag      600

gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag      660

gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat      720

atcaccgccg acaag                                                       735


<210>  10
<211>  245
<212>  PRT
<213>  Secuencia artificial

<220>
<223>  Venus cp157

<400>  10

Met Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile 
1               5                   10                  15      


Glu Asp Gly Gly Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro 
            20                  25                  30          


Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr 
        35                  40                  45              


Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val 
    50                  55                  60                  


Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu 
65                  70                  75                  80  


Leu Tyr Lys Gly Gly Ser Gly Gly Met Val Ser Lys Gly Glu Glu Leu 
                85                  90                  95      


Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn 
            100                 105                 110         


Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr 
        115                 120                 125             


Gly Lys Leu Thr Leu Lys Leu Ile Cys Thr Thr Gly Lys Leu Pro Val 
    130                 135                 140                 


Pro Trp Pro Thr Leu Val Thr Thr Leu Gly Tyr Gly Leu Met Cys Phe 
145                 150                 155                 160 


Ala Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala 
                165                 170                 175     


Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp 
            180                 185                 190         


Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu 
        195                 200                 205             


Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn 
    210                 215                 220                 


Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr 
225                 230                 235                 240 


Ile Thr Ala Asp Lys 
                245 


<210>  11
<211>  735
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  Venus cp173

<400>  11
atggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc       60

cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc      120

aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc      180

ggcatggacg agctgtacaa gggtggcagc ggtggcatgg tgagcaaggg cgaggagctg      240

ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc      300

agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagctgatc      360

tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gggctacggc      420

ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc      480

atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag      540

acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc      600

atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc      660

cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc      720

cgccacaaca tcgag                                                       735


<210>  12
<211>  245
<212>  PRT
<213>  Secuencia artificial

<220>
<223>  Venus cp173

<400>  12

Met Asp Gly Gly Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro 
1               5                   10                  15      


Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr 
            20                  25                  30          


Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val 
        35                  40                  45              


Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu 
    50                  55                  60                  


Leu Tyr Lys Gly Gly Ser Gly Gly Met Val Ser Lys Gly Glu Glu Leu 
65                  70                  75                  80  


Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn 
                85                  90                  95      


Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr 
            100                 105                 110         


Gly Lys Leu Thr Leu Lys Leu Ile Cys Thr Thr Gly Lys Leu Pro Val 
        115                 120                 125             


Pro Trp Pro Thr Leu Val Thr Thr Leu Gly Tyr Gly Leu Gln Cys Phe 
    130                 135                 140                 


Ala Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala 
145                 150                 155                 160 


Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp 
                165                 170                 175     


Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu 
            180                 185                 190         


Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn 
        195                 200                 205             


Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr 
    210                 215                 220                 


Ile Thr Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile 
225                 230                 235                 240 


Arg His Asn Ile Glu 
                245 


<210>  13
<211>  735
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  Venus cp195

<400>  13
atgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag       60

aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg      120

gacgagctgt acaagggtgg cagcggtggc atggtgagca agggcgagga gctgttcacc      180

ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg      240

tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagct gatctgcacc      300

accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgggcta cggcctgatg      360

tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc      420

gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc      480

gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac      540

ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac      600

gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac      660

aacatcgagg acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc      720

gacggccccg tgctg                                                       735


<210>  14
<211>  245
<212>  PRT
<213>  Secuencia artificial

<220>
<223>  Venus cp195

<400>  14

Met Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Ala Leu Ser Lys 
1               5                   10                  15      


Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr 
            20                  25                  30          


Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Gly Gly Ser 
        35                  40                  45              


Gly Gly Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro 
    50                  55                  60                  


Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val 
65                  70                  75                  80  


Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys 
                85                  90                  95      


Leu Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val 
            100                 105                 110         


Thr Thr Leu Gly Tyr Gly Leu Met Cys Phe Ala Arg Tyr Pro Asp His 
        115                 120                 125             


Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val 
    130                 135                 140                 


Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg 
145                 150                 155                 160 


Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu 
                165                 170                 175     


Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu 
            180                 185                 190         


Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln 
        195                 200                 205             


Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp 
    210                 215                 220                 


Gly Gly Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly 
225                 230                 235                 240 


Asp Gly Pro Val Leu 
                245 


<210>  15
<211>  735
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  Venus cp229

<400>  15
atgatcactc tcggcatgga cgagctgtac aagggtggca gcggtggcat ggtgagcaag       60

ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac      120

ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc      180

ctgaagctga tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc      240

ctgggctacg gcctgatgtg cttcgcccgc taccccgacc acatgaagca gcacgacttc      300

ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac      360

ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc      420

gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac      480

aactacaaca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc      540

aacttcaaga tccgccacaa catcgaggac ggcggcgtgc agctcgccga ccactaccag      600

cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac      660

cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc      720

gtgaccgccg ccggg                                                       735


<210>  16
<211>  245
<212>  PRT
<213>  Secuencia artificial

<220>
<223>  Venus cp229

<400>  16

Met Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Gly Gly Ser Gly Gly 
1               5                   10                  15      


Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
            20                  25                  30          


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
        35                  40                  45              


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Leu Ile 
    50                  55                  60                  


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
65                  70                  75                  80  


Leu Gly Tyr Gly Leu Met Cys Phe Ala Arg Tyr Pro Asp His Met Lys 
                85                  90                  95      


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
            100                 105                 110         


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
        115                 120                 125             


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
    130                 135                 140                 


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
145                 150                 155                 160 


Asn Tyr Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
                165                 170                 175     


Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Gly 
            180                 185                 190         


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
        195                 200                 205             


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Ala Leu 
    210                 215                 220                 


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
225                 230                 235                 240 


Val Thr Ala Ala Gly 
                245 


<210>  17
<211>  36
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  Lck (1-12)

<400>  17
atgggctgcg ggtgtagttc tcatccggaa gacgac                                 36


<210>  18
<211>  12
<212>  PRT
<213>  Secuencia artificial

<220>
<223>  Lck (1-12)

<400>  18

Met Gly Cys Gly Cys Ser Ser His Pro Glu Asp Asp 
1               5                   10          


<210>  19
<211>  1932
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  pmPAS

<400>  19
atgggctgcg ggtgtagttc tcatccggaa gacgacgcgg atcccgaagc agcagcaaga       60

gaagcagcag caagagaagc agcagcaaga gaagcagcag caagagaagc agcagcaaga      120

gaagcagcag caagagaagc agcagcaaga gcggatcccg tggtgagcaa gggcgaggag      180

ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacagg      240

ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc      300

atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg      360

ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc      420

gccatgcccg aaggctacgt ccaggagcgt accatcttct tcaaggacga cggcaactac      480

aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag      540

ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacatc      600

agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc ccacttcaag      660

atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc      720

cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc      780

ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc      840

gcccgcatgc gggaagcagc agcaagagaa gcagcagcaa gagaagcagc agcaagaggc      900

ggcgaagcag cagcaagaga agcagcagca agagaagcag cagcaagacg tacgggcgga      960

tctatggaca attgttcagg aagcagaaga cgtgataggc tacatgtgaa gcttaaatcc     1020

ttgaggaata aaatccacaa acaacttcac ccaaactgtc ggttcgatga cgccactaag     1080

actagtggag gatctcgtac ggaagcagca gcaagagaag cagcagcaag agaagcagca     1140

gcaagagaag cagcagcaag agaagcagca gcaagagaag cagcagcaag agagctcatg     1200

gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc     1260

gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac     1320

gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc     1380

atggacgagc tgtacaaggg tggcagcggt ggcatggtga gcaagggcga ggagctgttc     1440

accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc     1500

gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc     1560

accaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcctg     1620

cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg     1680

cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc     1740

cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc     1800

gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac     1860

aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc     1920

cacaacatcg ag                                                         1932


<210>  20
<211>  644
<212>  PRT
<213>  Secuencia artificial

<220>
<223>  pmPAS

<400>  20

Met Gly Cys Gly Cys Ser Ser His Pro Glu Asp Asp Ala Asp Pro Glu 
1               5                   10                  15      


Ala Ala Ala Arg Glu Ala Ala Ala Arg Glu Ala Ala Ala Arg Glu Ala 
            20                  25                  30          


Ala Ala Arg Glu Ala Ala Ala Arg Glu Ala Ala Ala Arg Glu Ala Ala 
        35                  40                  45              


Ala Arg Ala Asp Pro Val Val Ser Lys Gly Glu Glu Leu Phe Thr Gly 
    50                  55                  60                  


Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Arg 
65                  70                  75                  80  


Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu 
                85                  90                  95      


Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro 
            100                 105                 110         


Thr Leu Val Thr Thr Leu Thr Trp Gly Val Gln Cys Phe Ser Arg Tyr 
        115                 120                 125             


Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu 
    130                 135                 140                 


Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr 
145                 150                 155                 160 


Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg 
                165                 170                 175     


Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly 
            180                 185                 190         


His Lys Leu Glu Tyr Asn Tyr Ile Ser His Asn Val Tyr Ile Thr Ala 
        195                 200                 205             


Asp Lys Gln Lys Asn Gly Ile Lys Ala His Phe Lys Ile Arg His Asn 
    210                 215                 220                 


Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr 
225                 230                 235                 240 


Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser 
                245                 250                 255     


Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met 
            260                 265                 270         


Val Leu Leu Glu Phe Val Thr Ala Ala Arg Met Arg Glu Ala Ala Ala 
        275                 280                 285             


Arg Glu Ala Ala Ala Arg Glu Ala Ala Ala Arg Gly Gly Glu Ala Ala 
    290                 295                 300                 


Ala Arg Glu Ala Ala Ala Arg Glu Ala Ala Ala Arg Arg Thr Gly Gly 
305                 310                 315                 320 


Ser Met Asp Asn Cys Ser Gly Ser Arg Arg Arg Asp Arg Leu His Val 
                325                 330                 335     


Lys Leu Lys Ser Leu Arg Asn Lys Ile His Lys Gln Leu His Pro Asn 
            340                 345                 350         


Cys Arg Phe Asp Asp Ala Thr Lys Thr Ser Gly Gly Ser Arg Thr Glu 
        355                 360                 365             


Ala Ala Ala Arg Glu Ala Ala Ala Arg Glu Ala Ala Ala Arg Glu Ala 
    370                 375                 380                 


Ala Ala Arg Glu Ala Ala Ala Arg Glu Ala Ala Ala Arg Glu Leu Met 
385                 390                 395                 400 


Asp Gly Gly Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile 
                405                 410                 415     


Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln 
            420                 425                 430         


Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu 
        435                 440                 445             


Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu 
    450                 455                 460                 


Tyr Lys Gly Gly Ser Gly Gly Met Val Ser Lys Gly Glu Glu Leu Phe 
465                 470                 475                 480 


Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly 
                485                 490                 495     


His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly 
            500                 505                 510         


Lys Leu Thr Leu Lys Leu Ile Cys Thr Thr Gly Lys Leu Pro Val Pro 
        515                 520                 525             


Trp Pro Thr Leu Val Thr Thr Leu Gly Tyr Gly Leu Gln Cys Phe Ala 
    530                 535                 540                 


Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met 
545                 550                 555                 560 


Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly 
                565                 570                 575     


Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val 
            580                 585                 590         


Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile 
        595                 600                 605             


Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile 
    610                 615                 620                 


Thr Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg 
625                 630                 635                 640 


His Asn Ile Glu 
                


<210>  21
<211>  99
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  Tom20 (1-33)

<400>  21
atggtgggcc ggaacagcgc catcgccgcg ggcgtgtgcg gtgccctctt catagggtac       60

tgcatctact ttgaccgcaa aaggcggagt gaccccaac                              99


<210>  22
<211>  33
<212>  PRT
<213>  Secuencia artificial

<220>
<223>  Tom20 (1-33)

<400>  22

Met Val Gly Arg Asn Ser Ala Ile Ala Ala Gly Val Cys Gly Ala Leu 
1               5                   10                  15      


Phe Ile Gly Tyr Cys Ile Tyr Phe Asp Arg Lys Arg Arg Ser Asp Pro 
            20                  25                  30          


Asn 
    


<210>  23
<211>  1995
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  mitPAS

<400>  23
atggtgggcc ggaacagcgc catcgccgcg ggcgtgtgcg gtgccctctt catagggtac       60

tgcatctact ttgaccgcaa aaggcggagt gaccccaacg cggatcccga agcagcagca      120

agagaagcag cagcaagaga agcagcagca agagaagcag cagcaagaga agcagcagca      180

agagaagcag cagcaagaga agcagcagca agagcggtac ccgtggtgag caagggcgag      240

gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac      300

aggttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag      360

ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc      420

tggggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag      480

tccgccatgc ccgaaggcta cgtccaggag cgtaccatct tcttcaagga cgacggcaac      540

tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg      600

aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac      660

atcagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggcccacttc      720

aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac      780

acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc      840

gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc      900

gccgcccgca tgcgggaagc agcagcaaga gaagcagcag caagagaagc agcagcaaga      960

ggcggcgaag cagcagcaag agaagcagca gcaagagaag cagcagcaag acgtacgggc     1020

ggatctatgg acaattgttc aggaagcaga agacgtgata ggctacatgt gaagctgaaa     1080

tccttgagga ataaaatcca caaacaactt cacccaaact gtcggttcga tgacgccact     1140

aagactagtg gaggatctcg tacggaagca gcagcaagag aagcagcagc aagagaagca     1200

gcagcaagag aagcagcagc aagagaagca gcagcaagag aagcagcagc aagagagctc     1260

atggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc     1320

cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc     1380

aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc     1440

ggcatggacg agctgtacaa gggtggcagc ggtggcatgg tgagcaaggg cgaggagctg     1500

ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc     1560

agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagctgatc     1620

tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gggctacggc     1680

ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc     1740

atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag     1800

acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc     1860

atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc     1920

cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc     1980

cgccacaaca tcgag                                                      1995


<210>  24
<211>  665
<212>  PRT
<213>  Secuencia artificial

<220>
<223>  mitPAS

<400>  24

Met Val Gly Arg Asn Ser Ala Ile Ala Ala Gly Val Cys Gly Ala Leu 
1               5                   10                  15      


Phe Ile Gly Tyr Cys Ile Tyr Phe Asp Arg Lys Arg Arg Ser Asp Pro 
            20                  25                  30          


Asn Ala Asp Pro Glu Ala Ala Ala Arg Glu Ala Ala Ala Arg Glu Ala 
        35                  40                  45              


Ala Ala Arg Glu Ala Ala Ala Arg Glu Ala Ala Ala Arg Glu Ala Ala 
    50                  55                  60                  


Ala Arg Glu Ala Ala Ala Arg Ala Val Pro Val Val Ser Lys Gly Glu 
65                  70                  75                  80  


Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp 
                85                  90                  95      


Val Asn Gly His Arg Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala 
            100                 105                 110         


Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu 
        115                 120                 125             


Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Trp Gly Val Gln 
    130                 135                 140                 


Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys 
145                 150                 155                 160 


Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys 
                165                 170                 175     


Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp 
            180                 185                 190         


Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp 
        195                 200                 205             


Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Ile Ser His Asn 
    210                 215                 220                 


Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala His Phe 
225                 230                 235                 240 


Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His 
                245                 250                 255     


Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp 
            260                 265                 270         


Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu 
        275                 280                 285             


Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Arg Met 
    290                 295                 300                 


Arg Glu Ala Ala Ala Arg Glu Ala Ala Ala Arg Glu Ala Ala Ala Arg 
305                 310                 315                 320 


Gly Gly Glu Ala Ala Ala Arg Glu Ala Ala Ala Arg Glu Ala Ala Ala 
                325                 330                 335     


Arg Arg Thr Gly Gly Ser Met Asp Asn Cys Ser Gly Ser Arg Arg Arg 
            340                 345                 350         


Asp Arg Leu His Val Lys Leu Lys Ser Leu Arg Asn Lys Ile His Lys 
        355                 360                 365             


Gln Leu His Pro Asn Cys Arg Phe Asp Asp Ala Thr Lys Thr Ser Gly 
    370                 375                 380                 


Gly Ser Arg Thr Glu Ala Ala Ala Arg Glu Ala Ala Ala Arg Glu Ala 
385                 390                 395                 400 


Ala Ala Arg Glu Ala Ala Ala Arg Glu Ala Ala Ala Arg Glu Ala Ala 
                405                 410                 415     


Ala Arg Glu Leu Met Asp Gly Gly Val Gln Leu Ala Asp His Tyr Gln 
            420                 425                 430         


Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His 
        435                 440                 445             


Tyr Leu Ser Tyr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg 
    450                 455                 460                 


Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu 
465                 470                 475                 480 


Gly Met Asp Glu Leu Tyr Lys Gly Gly Ser Gly Gly Met Val Ser Lys 
                485                 490                 495     


Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp 
            500                 505                 510         


Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly 
        515                 520                 525             


Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Leu Ile Cys Thr Thr Gly 
    530                 535                 540                 


Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Gly Tyr Gly 
545                 550                 555                 560 


Leu Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln His Asp Phe 
                565                 570                 575     


Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe 
            580                 585                 590         


Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu 
        595                 600                 605             


Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys 
    610                 615                 620                 


Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser 
625                 630                 635                 640 


His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala 
                645                 650                 655     


Asn Phe Lys Ile Arg His Asn Ile Glu 
            660                 665 


<210>  25
<211>  105
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  oligonucletido SphI_Ln2_SphI_FW

<400>  25
cgggaagcag cagcaagaga agcagcagca agagaagcag cagcaagagg cggcgaagca       60

gcagcaagag aagcagcagc aagagaagca gcagcaagac gcatg                      105


<210>  26
<211>  105
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  oligonucletido SphI_Ln2_SphI_REV

<400>  26
cgtcttgctg ctgcttctct tgctgctgct tctcttgctg ctgcttcgcc gcctcttgct       60

gctgcttctc ttgctgctgc ttctcttgct gctgcttccc gcatg                      105


<210>  27
<211>  96
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  (oligonucletido SacI_Ln3_SacI_FW

<400>  27
cgaagcagca gcaagagaag cagcagcaag agaagcagca gcaagagaag cagcagcaag       60

agaagcagca gcaagagaag cagcagcaag agagct                                 96


<210>  28
<211>  96
<212>  DNA
<213>  Secuencia artificial
<220>
<223>  oligonucletido SacI_Ln3_SacI_REV

<400>  28
ctcttgctgc tgcttctctt gctgctgctt ctcttgctgc tgcttctctt gctgctgctt       60

ctcttgctgc tgcttctctt gctgctgctt cgagct                                 96


<210>  29
<211>  47
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  oligonucletido HIndIII_Lck(1-12)_BamHI_FW

<400>  29
agcttaccat gggctgcggg tgtagttctc atccggaaga cgacgcg                     47


<210>  30
<211>  47
<212>  DNA
<213> Secuencia artificial

<220>
<223>  oligonucletido HIndIII_Lck(1-12)_BamHI_REV

<400>  30
gatccgcgtc gtcttccgga tgagaactac acccgcagcc catggta                     47


<210>  31
<211>  114
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  oligonucletido BamHI_Ln1_BamHI_FW

<400>  31
gatcccgaag cagcagcaag agaagcagca gcaagagaag cagcagcaag agaagcagca       60

gcaagagaag cagcagcaag agaagcagca gcaagagaag cagcagcaag agcg            114


<210>  32
<211>  114
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  oligonucletido BamHI_Ln1_BamHI_REV

<400>  32
gatccgctct tgctgctgct tctcttgctg ctgcttctct tgctgctgct tctcttgctg       60

ctgcttctct tgctgctgct tctcttgctg ctgcttctct tgctgctgct tcgg            114


<210>  33
<211>  28
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  oligonucletido mutLn2_SphI>BsiWI

<400>  33
cagcagcaag acgtacgcgg gactgctg                                          28


<210>  34
<211>  35
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  oligonucletido mutSacI>BsiWI_Ln3

<400>  34
cccagcggag atgcgtacgg aagcagcagc aagag                                  35


<210>  35
<211>  37
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  oligonucletido BsiWI_Spo20(51-91)_BsiWI_FW

<400>  35
acattcgtac gggcggatct atggacaatt gttcagg                                37


<210>  36
<211>  36
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  oligonucletido BsiWI_Spo20(51-91)_BsiWI_REV

<400>  36
ctattcgtac gagatcctcc actagtctta gtggcg                                 36


<210>  37
<211>  27
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  oligonucletido mutECFP_M1V

<400>  37
caagagcgga tcccgtggtg agcaagg                                           27


<210>  38
<211>  29
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  oligonucletido mutSpo20_noHindIII

<400>  38
ggctacatgt gaagctgaaa tccttgagg                                         29


<210>  39
<211>  27
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  oligonucletido mutLn1end_BamHItoKpnI

<400>  39
gcagcaagag cggtacccgt ggtgagc                                           27


<210>  40
<211>  110
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  oligonucletido HindIII_Tom20(1-33)_BamHI_FW

<400>  40
agcttaccat ggtgggccgg aacagcgcca tcgccgcggg cgtgtgcggt gccctcttca       60

tagggtactg catctacttt gaccgcaaaa ggcggagtga ccccaacgcg                 110


<210>  41
<211>  110
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  oligonucletido HindIII_Tom20(1-33)_BamHI_REV

<400>  41
gatccgcgtt ggggtcactc cgccttttgc ggtcaaagta gatgcagtac cctatgaaga       60

gggcaccgca cacgcccgcg gcgatggcgc tgttccggcc caccatggta                 110


