                         SEQUENCE LISTING

<110>  The University of Sheffield
 
<120>  Biomarkers for Disease Stratification

<130>  4348P/WO

<150>  1316131.0
<151>  2013-09-11

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  miR-140-5p micro RNA

<400>  1
cagugguuuu acccuauggu ag                                                22


<210>  2
<211>  281
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Ala Met Met Glu Val Gln Gly Gly Pro Ser Leu Gly Gln Thr Cys 
1               5                   10                  15      


Val Leu Ile Val Ile Phe Thr Val Leu Leu Gln Ser Leu Cys Val Ala 
            20                  25                  30          


Val Thr Tyr Val Tyr Phe Thr Asn Glu Leu Lys Gln Met Gln Asp Lys 
        35                  40                  45              


Tyr Ser Lys Ser Gly Ile Ala Cys Phe Leu Lys Glu Asp Asp Ser Tyr 
    50                  55                  60                  


Trp Asp Pro Asn Asp Glu Glu Ser Met Asn Ser Pro Cys Trp Gln Val 
65                  70                  75                  80  


Lys Trp Gln Leu Arg Gln Leu Val Arg Lys Met Ile Leu Arg Thr Ser 
                85                  90                  95      


Glu Glu Thr Ile Ser Thr Val Gln Glu Lys Gln Gln Asn Ile Ser Pro 
            100                 105                 110         


Leu Val Arg Glu Arg Gly Pro Gln Arg Val Ala Ala His Ile Thr Gly 
        115                 120                 125             


Thr Arg Gly Arg Ser Asn Thr Leu Ser Ser Pro Asn Ser Lys Asn Glu 
    130                 135                 140                 


Lys Ala Leu Gly Arg Lys Ile Asn Ser Trp Glu Ser Ser Arg Ser Gly 
145                 150                 155                 160 


His Ser Phe Leu Ser Asn Leu His Leu Arg Asn Gly Glu Leu Val Ile 
                165                 170                 175     


His Glu Lys Gly Phe Tyr Tyr Ile Tyr Ser Gln Thr Tyr Phe Arg Phe 
            180                 185                 190         


Gln Glu Glu Ile Lys Glu Asn Thr Lys Asn Asp Lys Gln Met Val Gln 
        195                 200                 205             


Tyr Ile Tyr Lys Tyr Thr Ser Tyr Pro Asp Pro Ile Leu Leu Met Lys 
    210                 215                 220                 


Ser Ala Arg Asn Ser Cys Trp Ser Lys Asp Ala Glu Tyr Gly Leu Tyr 
225                 230                 235                 240 


Ser Ile Tyr Gln Gly Gly Ile Phe Glu Leu Lys Glu Asn Asp Arg Ile 
                245                 250                 255     


Phe Val Ser Val Thr Asn Glu His Leu Ile Asp Met Asp His Glu Ala 
            260                 265                 270         


Ser Phe Phe Gly Ala Phe Leu Val Gly 
        275                 280     


<210>  3
<211>  101
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Ala Met Met Glu Val Gln Gly Gly Pro Ser Leu Gly Gln Thr Cys 
1               5                   10                  15      


Val Leu Ile Val Ile Phe Thr Val Leu Leu Gln Ser Leu Cys Val Ala 
            20                  25                  30          


Val Thr Tyr Val Tyr Phe Thr Asn Glu Leu Lys Gln Met Gln Asp Lys 
        35                  40                  45              


Tyr Ser Lys Ser Gly Ile Ala Cys Phe Leu Lys Glu Asp Asp Ser Tyr 
    50                  55                  60                  


Trp Asp Pro Asn Asp Glu Glu Ser Met Asn Ser Pro Cys Trp Gln Val 
65                  70                  75                  80  


Lys Trp Gln Leu Arg Gln Leu Val Arg Lys Thr Pro Arg Met Lys Arg 
                85                  90                  95      


Leu Trp Ala Ala Lys 
            100     


<210>  4
<211>  65
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Ala Met Met Glu Val Gln Gly Gly Pro Ser Leu Gly Gln Thr Cys 
1               5                   10                  15      


Val Leu Ile Val Ile Phe Thr Val Leu Leu Gln Ser Leu Cys Val Ala 
            20                  25                  30          


Val Thr Tyr Val Tyr Phe Thr Asn Glu Leu Lys Gln Phe Ala Glu Asn 
        35                  40                  45              


Asp Cys Gln Arg Leu Met Ser Gly Gln Gln Thr Gly Ser Leu Leu Pro 
    50                  55                  60                  


Ser 
65  


<210>  5
<211>  401
<212>  PRT
<213>  Homo sapiens

<400>  5

Met Asn Asn Leu Leu Cys Cys Ala Leu Val Phe Leu Asp Ile Ser Ile 
1               5                   10                  15      


Lys Trp Thr Thr Gln Glu Thr Phe Pro Pro Lys Tyr Leu His Tyr Asp 
            20                  25                  30          


Glu Glu Thr Ser His Gln Leu Leu Cys Asp Lys Cys Pro Pro Gly Thr 
        35                  40                  45              


Tyr Leu Lys Gln His Cys Thr Ala Lys Trp Lys Thr Val Cys Ala Pro 
    50                  55                  60                  


Cys Pro Asp His Tyr Tyr Thr Asp Ser Trp His Thr Ser Asp Glu Cys 
65                  70                  75                  80  


Leu Tyr Cys Ser Pro Val Cys Lys Glu Leu Gln Tyr Val Lys Gln Glu 
                85                  90                  95      


Cys Asn Arg Thr His Asn Arg Val Cys Glu Cys Lys Glu Gly Arg Tyr 
            100                 105                 110         


Leu Glu Ile Glu Phe Cys Leu Lys His Arg Ser Cys Pro Pro Gly Phe 
        115                 120                 125             


Gly Val Val Gln Ala Gly Thr Pro Glu Arg Asn Thr Val Cys Lys Arg 
    130                 135                 140                 


Cys Pro Asp Gly Phe Phe Ser Asn Glu Thr Ser Ser Lys Ala Pro Cys 
145                 150                 155                 160 


Arg Lys His Thr Asn Cys Ser Val Phe Gly Leu Leu Leu Thr Gln Lys 
                165                 170                 175     


Gly Asn Ala Thr His Asp Asn Ile Cys Ser Gly Asn Ser Glu Ser Thr 
            180                 185                 190         


Gln Lys Cys Gly Ile Asp Val Thr Leu Cys Glu Glu Ala Phe Phe Arg 
        195                 200                 205             


Phe Ala Val Pro Thr Lys Phe Thr Pro Asn Trp Leu Ser Val Leu Val 
    210                 215                 220                 


Asp Asn Leu Pro Gly Thr Lys Val Asn Ala Glu Ser Val Glu Arg Ile 
225                 230                 235                 240 


Lys Arg Gln His Ser Ser Gln Glu Gln Thr Phe Gln Leu Leu Lys Leu 
                245                 250                 255     


Trp Lys His Gln Asn Lys Asp Gln Asp Ile Val Lys Lys Ile Ile Gln 
            260                 265                 270         


Asp Ile Asp Leu Cys Glu Asn Ser Val Gln Arg His Ile Gly His Ala 
        275                 280                 285             


Asn Leu Thr Phe Glu Gln Leu Arg Ser Leu Met Glu Ser Leu Pro Gly 
    290                 295                 300                 


Lys Lys Val Gly Ala Glu Asp Ile Glu Lys Thr Ile Lys Ala Cys Lys 
305                 310                 315                 320 


Pro Ser Asp Gln Ile Leu Lys Leu Leu Ser Leu Trp Arg Ile Lys Asn 
                325                 330                 335     


Gly Asp Gln Asp Thr Leu Lys Gly Leu Met His Ala Leu Lys His Ser 
            340                 345                 350         


Lys Thr Tyr His Phe Pro Lys Thr Val Thr Gln Ser Leu Lys Lys Thr 
        355                 360                 365             


Ile Arg Phe Leu His Ser Phe Thr Met Tyr Lys Leu Tyr Gln Lys Leu 
    370                 375                 380                 


Phe Leu Glu Met Ile Gly Asn Gln Val Gln Ser Val Lys Ile Ser Cys 
385                 390                 395                 400 


Leu 
    


<210>  6
<211>  2196
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA

<400>  6
atgtcgaacc ccgggacacg caggaacggc tccagcatca agatccgtct gacagtgtta       60

tgtgccaaga accttgcaaa gaaagacttc ttcaggctcc ctgacccttt tgcaaagatt      120

gtcgtggatg ggtctgggca gtgccactca accgacactg tgaaaaacac attggaccca      180

aagtggaacc agcactatga tctatatgtt gggaaaacgg attcgataac cattagcgtg      240

tggaaccata agaaaattca caagaaacag ggagctggct tcctgggctg tgtgcggctg      300

ctctccaatg ccatcagcag attaaaagat accggatacc agcgtttgga tctatgcaaa      360

ctaaacccct cagatactga tgcagttcgt ggccagatag tggtcagttt acagacacga      420

gacagaatag gaaccggcgg ctcggtggtg gactgcagag gactgttaga aaatgaagga      480

acggtgtatg aagactccgg gcctgggagg ccgctcagct gcttcatgga ggaaccagcc      540

ccttacacag atagcaccgg tgctgctgct ggaggaggga attgcaggtt cgtggagtcc      600

ccaagtcaag atcaaagact tcaggcacag cggcttcgaa accctgatgt gcgaggttca      660

ctacagacgc cccagaaccg accacacggc caccagtccc cggaactgcc cgaaggctac      720

gaacaaagaa caacagtcca gggccaagtt tactttttgc atacacagac tggagttagc      780

acgtggcacg accccaggat accaagagac cttaacagtg tgaactgtga tgaacttgga      840

ccactgccgc caggctggga agtcagaagt acagtttctg ggaggatata ttttgtagat      900

cataataacc gaacaaccca gtttacagac ccaaggttac accacatcat gaatcaccag      960

tgccaactca aggagcccag ccagccgctg ccactgccca gtgagggctc tctggaggac     1020

gaggagcttc ctgcccagag atacgaaaga gatctagtcc agaagctgaa agtcctcaga     1080

cacgaactgt cgcttcagca gccccaagct ggtcattgcc gcatcgaagt gtccagagaa     1140

gaaatctttg aggagtctta ccgccagata atgaagatgc gaccgaaaga cttgaaaaaa     1200

cggctgatgg tgaaattccg tggggaagaa ggtttggatt acggtggtgt ggccagggag     1260

tggctttact tgctgtgcca tgaaatgctg aatccttatt acgggctctt ccagtattct     1320

acggacaata tttacatgtt gcaaataaat ccggattctt caatcaaccc cgaccacttg     1380

tcttatttcc actttgtggg gcggatcatg gggctggctg tgttccatgg acactacatc     1440

aacgggggct tcacagtgcc cttctacaag cagctgctgg ggaagcccat ccagctctca     1500

gatctggaat ctgtggaccc agagctgcat aagagcttgg tgtggatcct agagaacgac     1560

atcacgcctg tactggacca caccttctgc gtggaacaca acgccttcgg gcggatcctg     1620

cagcatgaac tgaaacccaa tggcagaaat gtgccagtca cagaggagaa taagaaagaa     1680

tacgtccggt tgtatgtaaa ctggaggttt atgagaggaa tcgaagccca gttcttagct     1740

ctgcagaagg ggttcaatga gctcatccct caacatctgc tgaagccttt tgaccagaag     1800

gaactggagc tgatcatagg cggcctggat aaaatagact tgaacgactg gaagtcgaac     1860

acgcggctga agcactgtgt ggccgacagc aacatcgtgc ggtggttctg gcaagcggtg     1920

gagacgttcg atgaagaaag gagggccagg ctcctgcagt ttgtgactgg gtccacgcga     1980

gtcccgctcc aaggcttcaa ggctttgcaa ggttctacag gcgcggcagg gccccggctg     2040

ttcaccatcc acctgataga cgcgaacaca gacaaccttc cgaaggccca tacctgcttt     2100

aaccggatcg acattccacc atatgagtcc tatgagaagc tctacgagaa gctgctgaca     2160

gccgtggagg agacctgcgg gtttgctgtg gagtga                               2196


<210>  7
<211>  757
<212>  PRT
<213>  Homo sapiens

<400>  7

Met Ser Asn Pro Gly Thr Arg Arg Asn Gly Ser Ser Ile Lys Ile Arg 
1               5                   10                  15      


Leu Thr Val Leu Cys Ala Lys Asn Leu Ala Lys Lys Asp Phe Phe Arg 
            20                  25                  30          


Leu Pro Asp Pro Phe Ala Lys Ile Val Val Asp Gly Ser Gly Gln Cys 
        35                  40                  45              


His Ser Thr Asp Thr Val Lys Asn Thr Leu Asp Pro Lys Trp Asn Gln 
    50                  55                  60                  


His Tyr Asp Leu Tyr Val Gly Lys Thr Asp Ser Ile Thr Ile Ser Val 
65                  70                  75                  80  


Trp Asn His Lys Lys Ile His Lys Lys Gln Gly Ala Gly Phe Leu Gly 
                85                  90                  95      


Cys Val Arg Leu Leu Ser Asn Ala Ile Ser Arg Leu Lys Asp Thr Gly 
            100                 105                 110         


Tyr Gln Arg Leu Asp Leu Cys Lys Leu Asn Pro Ser Asp Thr Asp Ala 
        115                 120                 125             


Val Arg Gly Gln Ile Val Val Ser Leu Gln Thr Arg Asp Arg Ile Gly 
    130                 135                 140                 


Thr Gly Gly Ser Val Val Asp Cys Arg Gly Leu Leu Glu Asn Glu Gly 
145                 150                 155                 160 


Thr Val Tyr Glu Asp Ser Gly Pro Gly Arg Pro Leu Ser Cys Phe Met 
                165                 170                 175     


Glu Glu Pro Ala Pro Tyr Thr Asp Ser Thr Gly Ala Ala Ala Gly Gly 
            180                 185                 190         


Gly Asn Cys Arg Phe Val Glu Ser Pro Ser Gln Asp Gln Arg Leu Gln 
        195                 200                 205             


Ala Gln Arg Leu Arg Asn Pro Asp Val Arg Gly Ser Leu Gln Thr Pro 
    210                 215                 220                 


Gln Asn Arg Pro His Gly His Gln Ser Pro Glu Leu Pro Glu Gly Tyr 
225                 230                 235                 240 


Glu Gln Arg Thr Thr Val Gln Gly Gln Val Tyr Phe Leu His Thr Gln 
                245                 250                 255     


Thr Gly Val Ser Thr Trp His Asp Pro Arg Ile Pro Ser Pro Ser Gly 
            260                 265                 270         


Thr Ile Pro Gly Gly Asp Ala Ala Phe Leu Tyr Glu Phe Leu Leu Gln 
        275                 280                 285             


Gly His Thr Ser Glu Pro Arg Asp Leu Asn Ser Val Asn Cys Asp Glu 
    290                 295                 300                 


Leu Gly Pro Leu Pro Pro Gly Trp Glu Val Arg Ser Thr Val Ser Gly 
305                 310                 315                 320 


Arg Ile Tyr Phe Val Asp His Asn Asn Arg Thr Thr Gln Phe Thr Asp 
                325                 330                 335     


Pro Arg Leu His His Ile Met Asn His Gln Cys Gln Leu Lys Glu Pro 
            340                 345                 350         


Ser Gln Pro Leu Pro Leu Pro Ser Glu Gly Ser Leu Glu Asp Glu Glu 
        355                 360                 365             


Leu Pro Ala Gln Arg Tyr Glu Arg Asp Leu Val Gln Lys Leu Lys Val 
    370                 375                 380                 


Leu Arg His Glu Leu Ser Leu Gln Gln Pro Gln Ala Gly His Cys Arg 
385                 390                 395                 400 


Ile Glu Val Ser Arg Glu Glu Ile Phe Glu Glu Ser Tyr Arg Gln Ile 
                405                 410                 415     


Met Lys Met Arg Pro Lys Asp Leu Lys Lys Arg Leu Met Val Lys Phe 
            420                 425                 430         


Arg Gly Glu Glu Gly Leu Asp Tyr Gly Gly Val Ala Arg Glu Trp Leu 
        435                 440                 445             


Tyr Leu Leu Cys His Glu Met Leu Asn Pro Tyr Tyr Gly Leu Phe Gln 
    450                 455                 460                 


Tyr Ser Thr Asp Asn Ile Tyr Met Leu Gln Ile Asn Pro Asp Ser Ser 
465                 470                 475                 480 


Ile Asn Pro Asp His Leu Ser Tyr Phe His Phe Val Gly Arg Ile Met 
                485                 490                 495     


Gly Leu Ala Val Phe His Gly His Tyr Ile Asn Gly Gly Phe Thr Val 
            500                 505                 510         


Pro Phe Tyr Lys Gln Leu Leu Gly Lys Pro Ile Gln Leu Ser Asp Leu 
        515                 520                 525             


Glu Ser Val Asp Pro Glu Leu His Lys Ser Leu Val Trp Ile Leu Glu 
    530                 535                 540                 


Asn Asp Ile Thr Pro Val Leu Asp His Thr Phe Cys Val Glu His Asn 
545                 550                 555                 560 


Ala Phe Gly Arg Ile Leu Gln His Glu Leu Lys Pro Asn Gly Arg Asn 
                565                 570                 575     


Val Pro Val Thr Glu Glu Asn Lys Lys Glu Tyr Val Arg Leu Tyr Val 
            580                 585                 590         


Asn Trp Arg Phe Met Arg Gly Ile Glu Ala Gln Phe Leu Ala Leu Gln 
        595                 600                 605             


Lys Gly Phe Asn Glu Leu Ile Pro Gln His Leu Leu Lys Pro Phe Asp 
    610                 615                 620                 


Gln Lys Glu Leu Glu Leu Ile Ile Gly Gly Leu Asp Lys Ile Asp Leu 
625                 630                 635                 640 


Asn Asp Trp Lys Ser Asn Thr Arg Leu Lys His Cys Val Ala Asp Ser 
                645                 650                 655     


Asn Ile Val Arg Trp Phe Trp Gln Ala Val Glu Thr Phe Asp Glu Glu 
            660                 665                 670         


Arg Arg Ala Arg Leu Leu Gln Phe Val Thr Gly Ser Thr Arg Val Pro 
        675                 680                 685             


Leu Gln Gly Phe Lys Ala Leu Gln Gly Ser Thr Gly Ala Ala Gly Pro 
    690                 695                 700                 


Arg Leu Phe Thr Ile His Leu Ile Asp Ala Asn Thr Asp Asn Leu Pro 
705                 710                 715                 720 


Lys Ala His Thr Cys Phe Asn Arg Ile Asp Ile Pro Pro Tyr Glu Ser 
                725                 730                 735     


Tyr Glu Lys Leu Tyr Glu Lys Leu Leu Thr Ala Val Glu Glu Thr Cys 
            740                 745                 750         


Gly Phe Ala Val Glu 
        755         


<210>  8
<211>  106
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U6 snRNA

<400>  8
gtgctcgctt cggcagcaca tatactaaaa ttggaacgat acagagaaga ttagcatggc       60

ccctgcgcaa ggatgacacg caaattcgtg aagcgttcca tatttt                     106


