                         SEQUENCE LISTING

<110>  INRIA INSTITUT NATIONAL DE RECHERCHE EN INFORMATIQUE ET EN 
       AUTOMATIQUE
       UNIVERSITE JOSEPH FOURIER - GRENOBLE 1
 
<120>  METHOD FOR PRODUCING METABOLITES, PEPTIDES AND RECOMBINANT 
       PROTEINS

<130>  BET 14P2222

<150>  EP13306266.1
<151>  2013-09-16

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  4029
<212>  DNA
<213>  Escherichia coli

<400>  1
atggtttact cctataccga gaaaaaacgt attcgtaagg attttggtaa acgtccacaa       60

gttctggatg taccttatct cctttctatc cagcttgact cgtttcagaa atttatcgag      120

caagatcctg aagggcagta tggtctggaa gctgctttcc gttccgtatt cccgattcag      180

agctacagcg gtaattccga gctgcaatac gtcagctacc gccttggcga accggtgttt      240

gacgtccagg aatgtcaaat ccgtggcgtg acctattccg caccgctgcg cgttaaactg      300

cgtctggtga tctatgagcg cgaagcgccg gaaggcaccg taaaagacat taaagaacaa      360

gaagtctaca tgggcgaaat tccgctcatg acagacaacg gtacctttgt tatcaacggt      420

actgagcgtg ttatcgtttc ccagctgcac cgtagtccgg gcgtcttctt tgactccgac      480

aaaggtaaaa cccactcttc gggtaaagtg ctgtataacg cgcgtatcat cccttaccgt      540

ggttcctggc tggacttcga attcgatccg aaggacaacc tgttcgtacg tatcgaccgt      600

cgccgtaaac tgcctgcgac catcattctg cgcgccctga actacaccac agagcagatc      660

ctcgacctgt tctttgaaaa agttatcttt gaaatccgtg ataacaagct gcagatggaa      720

ctggtgccgg aacgcctgcg tggtgaaacc gcatcttttg acatcgaagc taacggtaaa      780

gtgtacgtag aaaaaggccg ccgtatcact gcgcgccaca ttcgccagct ggaaaaagac      840

gacgtcaaac tgatcgaagt cccggttgag tacatcgcag gtaaagtggt tgctaaagac      900

tatattgatg agtctaccgg cgagctgatc tgcgcagcga acatggagct gagcctggat      960

ctgctggcta agctgagcca gtctggtcac aagcgtatcg aaacgctgtt caccaacgat     1020

ctggatcacg gcccatatat ctctgaaacc ttacgtgtcg acccaactaa cgaccgtctg     1080

agcgcactgg tagaaatcta ccgcatgatg cgccctggcg agccgccgac tcgtgaagca     1140

gctgaaagcc tgttcgagaa cctgttcttc tccgaagacc gttatgactt gtctgcggtt     1200

ggtcgtatga agttcaaccg ttctctgctg cgcgaagaaa tcgaaggttc cggtatcctg     1260

agcaaagacg acatcattga tgttatgaaa aagctcatcg atatccgtaa cggtaaaggc     1320

gaagtcgatg atatcgacca cctcggcaac cgtcgtatcc gttccgttgg cgaaatggcg     1380

gaaaaccagt tccgcgttgg cctggtacgt gtagagcgtg cggtgaaaga gcgtctgtct     1440

ctgggcgatc tggataccct gatgccacag gatatgatca acgccaagcc gatttccgca     1500

gcagtgaaag agttcttcgg ttccagccag ctgtctcagt ttatggacca gaacaacccg     1560

ctgtctgaga ttacgcacaa acgtcgtatc tccgcactcg gcccaggcgg tctgacccgt     1620

gaacgtgcag gcttcgaagt tcgagacgta cacccgactc actacggtcg cgtatgtcca     1680

atcgaaaccc ctgaaggtcc gaacatcggt ctgatcaact ctctgtccgt gtacgcacag     1740

actaacgaat acggcttcct tgagactccg tatcgtaaag tgaccgacgg tgttgtaact     1800

gacgaaattc actacctgtc tgctatcgaa gaaggcaact acgttatcgc ccaggcgaac     1860

tccaacttgg atgaagaagg ccacttcgta gaagacctgg taacttgccg tagcaaaggc     1920

gaatccagct tgttcagccg cgaccaggtt gactacatgg acgtatccac ccagcaggtg     1980

gtatccgtcg gtgcgtccct gatcccgttc ctggaacacg atgacgccaa ccgtgcattg     2040

atgggtgcga acatgcaacg tcaggccgtt ccgactctgc gcgctgataa gccgctggtt     2100

ggtactggta tggaacgtgc tgttgccgtt gactccggtg taactgcggt agctaaacgt     2160

ggtggtgtcg ttcagtacgt ggatgcttcc cgtatcgtta tcaaagttaa cgaagacgag     2220

atgtatccgg gtgaagcagg tatcgacatc tacaacctga ccaaatacac ccgttctaac     2280

cagaacacct gtatcaacca gatgccgtgt gtgtctctgg gtgaaccggt tgaacgtggc     2340

gacgtgctgg cagacggtcc gtccaccgac ctcggtgaac tggcgcttgg tcagaacatg     2400

cgcgtagcgt tcatgccgtg gaatggttac aacttcgaag actccatcct cgtatccgag     2460

cgtgttgttc aggaagaccg tttcaccacc atccacattc aggaactggc gtgtgtgtcc     2520

cgtgacacca agctgggtcc ggaagagatc accgctgaca tcccgaacgt gggtgaagct     2580

gcgctctcca aactggatga atccggtatc gtttacattg gtgcggaagt gaccggtggc     2640

gacattctgg ttggtaaggt aacgccgaaa ggtgaaactc agctgacccc agaagaaaaa     2700

ctgctgcgtg cgatcttcgg tgagaaagcc tctgacgtta aagactcttc tctgcgcgta     2760

ccaaacggtg tatccggtac ggttatcgac gttcaggtct ttactcgcga tggcgtagaa     2820

aaagacaaac gtgcgctgga aatcgaagaa atgcagctca aacaggcgaa gaaagacctg     2880

tctgaagaac tgcagatcct cgaagcgggt ctgttcagcc gtatccgtgc tgtgctggta     2940

gccggtggcg ttgaagctga gaagctcgac aaactgccgc gcgatcgctg gctggagctg     3000

ggcctgacag acgaagagaa acaaaatcag ctggaacagc tggctgagca gtatgacgaa     3060

ctgaaacacg agttcgagaa gaaactcgaa gcgaaacgcc gcaaaatcac ccagggcgac     3120

gatctggcac cgggcgtgct gaagattgtt aaggtatatc tggcggttaa acgccgtatc     3180

cagcctggtg acaagatggc aggtcgtcac ggtaacaagg gtgtaatttc taagatcaac     3240

ccgatcgaag atatgcctta cgatgaaaac ggtacgccgg tagacatcgt actgaacccg     3300

ctgggcgtac cgtctcgtat gaacatcggt cagatcctcg aaacccacct gggtatggct     3360

gcgaaaggta tcggcgacaa gatcaacgcc atgctgaaac agcagcaaga agtcgcgaaa     3420

ctgcgcgaat tcatccagcg tgcgtacgat ctgggcgctg acgttcgtca gaaagttgac     3480

ctgagtacct tcagcgatga agaagttatg cgtctggctg aaaacctgcg caaaggtatg     3540

ccaatcgcaa cgccggtgtt cgacggtgcg aaagaagcag aaattaaaga gctgctgaaa     3600

cttggcgacc tgccgacttc cggtcagatc cgcctgtacg atggtcgcac tggtgaacag     3660

ttcgagcgtc cggtaaccgt tggttacatg tacatgctga aactgaacca cctggtcgac     3720

gacaagatgc acgcgcgttc caccggttct tacagcctgg ttactcagca gccgctgggt     3780

ggtaaggcac agttcggtgg tcagcgtttc ggggagatgg aagtgtgggc gctggaagca     3840

tacggcgcag catacaccct gcaggaaatg ctcaccgtta agtctgatga cgtgaacggt     3900

cgtaccaaga tgtataaaaa catcgtggac ggcaaccatc agatggagcc gggcatgcca     3960

gaatccttca acgtattgtt gaaagagatt cgttcgctgg gtatcaacat cgaactggaa     4020

gacgagtaa                                                             4029


<210>  2
<211>  4224
<212>  DNA
<213>  Escherichia coli

<400>  2
gtgaaagatt tattaaagtt tctgaaagcg cagactaaaa ccgaagagtt tgatgcgatc       60

aaaattgctc tggcttcgcc agacatgatc cgttcatggt ctttcggtga agttaaaaag      120

ccggaaacca tcaactaccg tacgttcaaa ccagaacgtg acggcctttt ctgcgcccgt      180

atctttgggc cggtaaaaga ttacgagtgc ctgtgcggta agtacaagcg cctgaaacac      240

cgtggcgtca tctgtgagaa gtgcggcgtt gaagtgaccc agactaaagt acgccgtgag      300

cgtatgggcc acatcgaact ggcttccccg actgcgcaca tctggttcct gaaatcgctg      360

ccgtcccgta tcggtctgct gctcgatatg ccgctgcgcg atatcgaacg cgtactgtac      420

tttgaatcct atgtggttat cgaaggcggt atgaccaacc tggaacgtca gcagatcctg      480

actgaagagc agtatctgga cgcgctggaa gagttcggtg acgaattcga cgcgaagatg      540

ggggcggaag caatccaggc tctgctgaag agcatggatc tggagcaaga gtgcgaacag      600

ctgcgtgaag agctgaacga aaccaactcc gaaaccaagc gtaaaaagct gaccaagcgt      660

atcaaactgc tggaagcgtt cgttcagtct ggtaacaaac cagagtggat gatcctgacc      720

gttctgccgg tactgccgcc agatctgcgt ccgctggttc cgctggatgg tggtcgtttc      780

gcgacttctg acctgaacga tctgtatcgt cgcgtcatta accgtaacaa ccgtctgaaa      840

cgtctgctgg atctggctgc gccggacatc atcgtacgta acgaaaaacg tatgctgcag      900

gaagcggtag acgccctgct ggataacggt cgtcgcggtc gtgcgatcac cggttctaac      960

aagcgtcctc tgaaatcttt ggccgacatg atcaaaggta aacagggtcg tttccgtcag     1020

aacctgctcg gtaagcgtgt tgactactcc ggtcgttctg taatcaccgt aggtccatac     1080

ctgcgtctgc atcagtgcgg tctgccgaag aaaatggcac tggagctgtt caaaccgttc     1140

atctacggca agctggaact gcgtggtctt gctaccacca ttaaagctgc gaagaaaatg     1200

gttgagcgcg aagaagctgt cgtttgggat atcctggacg aagttatccg cgaacacccg     1260

gtactgctga accgtgcacc gactctgcac cgtctgggta tccaggcatt tgaaccggta     1320

ctgatcgaag gtaaagctat ccagctgcac ccgctggttt gtgcggcata taacgccgac     1380

ttcgatggtg accagatggc tgttcacgta ccgctgacgc tggaagccca gctggaagcg     1440

cgtgcgctga tgatgtctac caacaacatc ctgtccccgg cgaacggcga accaatcatc     1500

gttccgtctc aggacgttgt actgggtctg tactacatga cccgtgactg tgttaacgcc     1560

aaaggcgaag gcatggtgct gactggcccg aaagaagcag aacgtctgta tcgctctggt     1620

ctggcttctc tgcatgcgcg cgttaaagtg cgtatcaccg agtatgaaaa agatgctaac     1680

ggtgaattag tagcgaaaac cagcctgaaa gacacgactg ttggccgtgc cattctgtgg     1740

atgattgtac cgaaaggtct gccttactcc atcgtcaacc aggcgctggg taaaaaagca     1800

atctccaaaa tgctgaacac ctgctaccgc attctcggtc tgaaaccgac cgttattttt     1860

gcggaccaga tcatgtacac cggcttcgcc tatgcagcgc gttctggtgc atctgttggt     1920

atcgatgaca tggtcatccc ggagaagaaa cacgaaatca tctccgaggc agaagcagaa     1980

gttgctgaaa ttcaggagca gttccagtct ggtctggtaa ctgcgggcga acgctacaac     2040

aaagttatcg atatctgggc tgcggcgaac gatcgtgtat ccaaagcgat gatggataac     2100

ctgcaaactg aaaccgtgat taaccgtgac ggtcaggaag agaagcaggt ttccttcaac     2160

agcatctaca tgatggccga ctccggtgcg cgtggttctg cggcacagat tcgtcagctt     2220

gctggtatgc gtggtctgat ggcgaagccg gatggctcca tcatcgaaac gccaatcacc     2280

gcgaacttcc gtgaaggtct gaacgtactc cagtacttca tctccaccca cggtgctcgt     2340

aaaggtctgg cggataccgc actgaaaact gcgaactccg gttacctgac tcgtcgtctg     2400

gttgacgtgg cgcaggacct ggtggttacc gaagacgatt gtggtaccca tgaaggtatc     2460

atgatgactc cggttatcga gggtggtgac gttaaagagc cgctgcgcga tcgcgtactg     2520

ggtcgtgtaa ctgctgaaga cgttctgaag ccgggtactg ctgatatcct cgttccgcgc     2580

aacacgctgc tgcacgaaca gtggtgtgac ctgctggaag agaactctgt cgacgcggtt     2640

aaagtacgtt ctgttgtatc ttgtgacacc gactttggtg tatgtgcgca ctgctacggt     2700

cgtgacctgg cgcgtggcca catcatcaac aagggtgaag caatcggtgt tatcgcggca     2760

cagtccatcg gtgaaccggg tacacagctg accatgcgta cgttccacat cggtggtgcg     2820

gcatctcgtg cggctgctga atccagcatc caagtgaaaa acaaaggtag catcaagctc     2880

agcaacgtga agtcggttgt gaactccagc ggtaaactgg ttatcacttc ccgtaatact     2940

gaactgaaac tgatcgacga attcggtcgt actaaagaaa gctacaaagt accttacggt     3000

gcggtactgg cgaaaggcga tggcgaacag gttgctggcg gcgaaaccgt tgcaaactgg     3060

gacccgcaca ccatgccggt tatcaccgaa gtaagcggtt ttgtacgctt tactgacatg     3120

atcgacggcc agaccattac gcgtcagacc gacgaactga ccggtctgtc ttcgctggtg     3180

gttctggatt ccgcagaacg taccgcaggt ggtaaagatc tgcgtccggc actgaaaatc     3240

gttgatgctc agggtaacga cgttctgatc ccaggtaccg atatgccagc gcagtacttc     3300

ctgccgggta aagcgattgt tcagctggaa gatggcgtac agatcagctc tggtgacacc     3360

ctggcgcgta ttccgcagga atccggcggt accaaggaca tcaccggtgg tctgccgcgc     3420

gttgcggacc tgttcgaagc acgtcgtccg aaagagccgg caatcctggc tgaaatcagc     3480

ggtatcgttt ccttcggtaa agaaaccaaa ggtaaacgtc gtctggttat caccccggta     3540

gacggtagcg atccgtacga agagatgatt ccgaaatggc gtcagctcaa cgtgttcgaa     3600

ggtgaacgtg tagaacgtgg tgacgtaatt tccgacggtc cggaagcgcc gcacgacatt     3660

ctgcgtctgc gtggtgttca tgctgttact cgttacatcg ttaacgaagt acaggacgta     3720

taccgtctgc agggcgttaa gattaacgat aaacacatcg aagttatcgt tcgtcagatg     3780

ctgcgtaaag ctaccatcgt taacgcgggt agctccgact tcctggaagg cgaacaggtt     3840

gaatactctc gcgtcaagat cgcaaaccgc gaactggaag cgaacggcaa agtgggtgca     3900

acttactccc gcgatctgct gggtatcacc aaagcgtctc tggcaaccga gtccttcatc     3960

tccgcggcat cgttccagga gaccactcgc gtgctgaccg aagcagccgt tgcgggcaaa     4020

cgcgacgaac tgcgcggcct gaaagagaac gttatcgtgg gtcgtctgat cccggcaggt     4080

accggttacg cgtaccacca ggatcgtatg cgtcgccgtg ctgcgggtga agctccggct     4140

gcaccgcagg tgactgcaga agacgcatct gccagcctgg cagaactgct gaacgcaggt     4200

ctgggcggtt ctgataacga gtaa                                            4224


<210>  3
<211>  1083
<212>  DNA
<213>  Escherichia coli

<400>  3
gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt       60

tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg      120

gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag      180

tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc      240

gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa      300

cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt      360

gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc      420

actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt      480

ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag      540

caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc      600

tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg      660

agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact      720

gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc      780

gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca      840

tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc      900

gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc      960

gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc     1020

gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag     1080

tga                                                                   1083


<210>  4
<211>  1284
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cloning cassette


<220>
<221>  misc_feature
<222>  (1)..(50)
<223>  chromosome homology

<220>
<221>  terminator
<222>  (61)..(104)
<223>  rrnBt1

<220>
<221>  misc_feature
<222>  (117)..(910)
<223>  spcR selection cassette

<220>
<221>  misc_feature
<222>  (298)..(298)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (313)..(313)
<223>  n is a, c, g, or t

<220>
<221>  -35_signal
<222>  (1135)..(1140)

<220>
<221>  misc_feature
<222>  (1140)..(1157)
<223>  binding site - lacO1

<220>
<221>  -10_signal
<222>  (1158)..(1163)

<220>
<221>  misc_feature
<222>  (1170)..(1197)
<223>  binding site - lacO1

<220>
<221>  RBS
<222>  (1207)..(1217)

<220>
<221>  misc_feature
<222>  (1225)..(1284)
<223>  rpoB homology

<220>
<221>  misc_feature
<222>  (1269)..(1269)
<223>  n is a, c, g, or t

<400>  4
cgctgtaagg cgccagtagc gtttcacact gtttgactac tgctgtgcct acgtctcgag       60

ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttataattgt tagacattat      120

ttgccggcta ccttggtgat ctcgcccttc acgtagtgga caaattcttc caactgatct      180

gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag cctgtctagc ttcaagtatg      240

acgggctgat gctgggccgg caggcgctcc attgcccagt cggcagcgac atccttcngg      300

cgcgattttg ccnggttact gcgctgtacc aaatgcggga caacgtaagc actacatttc      360

gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt taaggtttca tttagcgcct      420

caaatagatc ctgttcagga accggatcaa agagttcctc cgccgctgga cctaccaagg      480

caacgctatg ttctcttgct tttgtcagca agatagccag atcaatgtcg atcgtggctg      540

gctcgaagat acctgcaaga atgtcattgc gctgccattc tccaaattgc agttcgcgct      600

tagctggata acgccacggg atgatgtcgt cgtgcacaac aatggtgact tctacagcgc      660

ggagaatctc gctctctcca ggggaagccg aagtttccaa aaggtcgttg atcaaagctc      720

gccgcgttgt ttcgtcaagc cttacggtca ccgtaaccag caaatcaata tcactgtgtg      780

gcttcaggcc gccatccact gcggagccgt acaaatgtac ggccagcaac gtcggttcga      840

gatggcgctc gatgacgcca actacctctg atagttgagt cgatacttcg gcgatcaccg      900

cttccctcat gatgtttaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc      960

tgctccataa catcaaacat cgacccaggg cgtaacgcgc ttgctgcttg gatgcccgag     1020

gcatagactg taccccaaaa aaacagtcat agcaagccat gaaaaccgcc actgcgccgt     1080

taccaccgct gcgttcggcc aaggttctgg ctcgagaaat cataaaaaat ttatttgctt     1140

tgtgagcgga taacaattac aatagattca attgtaagcg gataacaatt tcacacagaa     1200

ttcattaaag aggagaaatt aactatggtt tactcctata ccgagaaaaa acgtattcgt     1260

aaggatttng gtaaacgtcc acaa                                            1284


<210>  5
<211>  1277
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LacI sequence inserted at galK locus


<220>
<221>  misc_feature
<222>  (1)..(50)
<223>  homology start galk

<220>
<221>  -35_signal
<222>  (71)..(76)

<220>
<221>  -10_signal
<222>  (96)..(101)

<220>
<221>  gene
<222>  (125)..(1207)
<223>  lacI

<220>
<221>  misc_feature
<222>  (1230)..(1277)
<223>  homology end galK

<400>  5
gtttgcgcgc agtcagcgat atccattttc gcgaatccgg agtgtaagaa taagctagcg       60

tgacgatgcg ttgacatatc actgtgattc acatataata tgcgaaatca gaagagtatt      120

gctaatgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac      180

cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga      240

agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa      300

acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat      360

tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt      420

agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt      480

cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc      540

ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat      600

tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca      660

ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc      720

tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga      780

ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc      840

cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga      900

gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag      960

ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac     1020

cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt     1080

gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc     1140

ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg     1200

gcagtgacta aggtgatcag ctagttacgg aagagctggt gcctgccgta cagcaagctg     1260

tcgctgaaca atatgaa                                                    1277


<210>  6
<211>  1277
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LacI sequence inserted at intS locus


<220>
<221>  misc_feature
<222>  (1)..(49)
<223>  homology start of intS

<220>
<221>  -35_signal
<222>  (71)..(76)

<220>
<221>  -10_signal
<222>  (94)..(99)

<220>
<221>  gene
<222>  (125)..(1207)
<223>  lacI

<220>
<221>  misc_feature
<222>  (1228)..(1277)
<223>  homology end of intS

<400>  6
ccgtagattt acagttcgtc atggttcgct tcagatcgtt gacagccgca taagctagcg       60

tgacgatgcg ttgacatatc actgtgattc acatataata tgcgaaatca gaagagtatt      120

gctaatgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac      180

cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga      240

agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa      300

acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat      360

tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt      420

agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt      480

cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc      540

ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat      600

tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca      660

ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc      720

tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga      780

ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc      840

cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga      900

gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag      960

ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac     1020

cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt     1080

gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc     1140

ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg     1200

gcagtgacta aggtgatcag ctagttatgg gcggactggc ttgatgagaa ggtggagtga     1260

gcgaccttaa caactat                                                    1277


<210>  7
<211>  1282
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cloning cassette


<220>
<221>  misc_feature
<222>  (1)..(50)
<223>  Chromosome homology

<220>
<221>  terminator
<222>  (61)..(104)
<223>  rrnBt1

<220>
<221>  misc_feature
<222>  (117)..(908)
<223>  spcR selection cassette

<220>
<221>  -35_signal
<222>  (1133)..(1138)

<220>
<221>  misc_feature
<222>  (1138)..(1155)
<223>  binding site - lacO1

<220>
<221>  -10_signal
<222>  (1156)..(1161)

<220>
<221>  misc_feature
<222>  (1168)..(1195)
<223>  binding site - lacO1

<220>
<221>  RBS
<222>  (1205)..(1215)

<220>
<221>  misc_feature
<222>  (1223)..(1282)
<223>  rpoB homology

<400>  7
cgctgtaagg cgccagtagc gtttcacact gtttgactac tgctgtgcct acgtctcgag       60

ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttataattgt tagacattat      120

ttgccggcta ccttggtgat ctcgcccttc acgtagtgga caaattcttc caactgatct      180

gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag cctgtctagc ttcaagtatg      240

acgggctgat gctgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc      300

gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc      360

tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca      420

aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca      480

acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc      540

tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta      600

gctggataac gccacgggat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg      660

agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc      720

cgcgttgttt cgtcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc      780

ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga      840

tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct      900

tccctcatga tgtttaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg      960

ctccataaca tcaaacatcg acccagggcg taacgcgctt gctgcttgga tgcccgaggc     1020

atagactgta ccccaaaaaa acagtcatag caagccatga aaaccgccac tgcgccgtta     1080

ccaccgctgc gttcggccaa ggttctggct cgagaaatca taaaaaattt atttgctttg     1140

tgagcggata acaattacaa tagattcaat tgtaagcgga taacaatttc acacagaatt     1200

cattaaagag gagaaattaa ctatggttta ctcctatacc gagaaaaaac gtattcgtaa     1260

ggattttggt aaacgtccac aa                                              1282


